AlfalfaGEDB | Msa0315300

M. sativa cultivar zhongmu-4 / Msa0315300

KEGG: -
GO: -
PFAM: PF00190.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0315300	XP_003593910.1	98.643	221	3	0	1	221	1	221	2.24e-156	442

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0315300	sp\|Q9SFF9\|GL17_ARATH	65.778	225	69	4	4	221	5	228	4.94e-96	282

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006700	Msa0315300	-0.821663	3.582317e-53	-8.615850e-47
Msa0008850	Msa0315300	-0.818019	2.437912e-52	-8.615850e-47
Msa0012780	Msa0315300	0.812478	4.155270e-51	-8.615850e-47
Msa0012820	Msa0315300	0.864161	1.483453e-64	-8.615850e-47
Msa0017120	Msa0315300	-0.800659	1.299302e-48	-8.615850e-47
Msa0020630	Msa0315300	-0.825781	3.886796e-54	-8.615850e-47
Msa0036230	Msa0315300	0.826063	3.332392e-54	-8.615850e-47
Msa0036580	Msa0315300	0.864523	1.143960e-64	-8.615850e-47
Msa0043940	Msa0315300	-0.800587	1.344105e-48	-8.615850e-47
Msa0047470	Msa0315300	-0.815640	8.336560e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0315300	MtrunA17_Chr2g0285871	98.643	221	3	0	1	221	1	221	2.06e-160	442
Msa0315300	MtrunA17_Chr4g0007031	98.190	221	4	0	1	221	1	221	2.49e-160	442
Msa0315300	MtrunA17_Chr5g0419971	98.190	221	4	0	1	221	1	221	2.49e-160	442
Msa0315300	MtrunA17_Chr5g0419981	98.190	221	4	0	1	221	1	221	2.49e-160	442
Msa0315300	MtrunA17_Chr4g0007051	97.738	221	5	0	1	221	1	221	9.91e-160	440
Msa0315300	MtrunA17_Chr4g0007041	96.833	221	7	0	1	221	1	221	4.77e-158	436
Msa0315300	MtrunA17_Chr1g0189841	79.279	222	45	1	1	221	1	222	2.13e-129	363
Msa0315300	MtrunA17_Chr6g0449771	77.523	218	47	2	4	221	5	220	1.88e-118	336
Msa0315300	MtrunA17_Chr6g0449751	77.027	222	48	3	1	221	1	220	2.92e-118	335
Msa0315300	MtrunA17_Chr6g0449731	77.419	217	47	2	4	220	5	219	6.63e-118	334

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0315300	AT3G05950.1	65.778	225	69	4	4	221	5	228	5.02e-97	282
Msa0315300	AT5G39150.1	63.303	218	77	2	1	215	1	218	6.17e-94	274
Msa0315300	AT5G39180.1	63.303	218	77	2	1	215	1	218	1.26e-93	273
Msa0315300	AT5G39120.1	64.455	211	72	2	7	215	9	218	2.12e-93	273
Msa0315300	AT5G39110.1	63.981	211	74	1	7	215	9	219	3.00e-92	270
Msa0315300	AT5G39160.1	63.927	219	74	3	1	215	1	218	3.81e-92	270
Msa0315300	AT5G39130.1	65.072	209	70	2	9	215	11	218	5.18e-92	269
Msa0315300	AT5G39190.1	63.927	219	74	3	1	215	1	218	5.47e-92	269
Msa0315300	AT4G14630.1	61.187	219	81	2	1	215	3	221	5.82e-88	259
Msa0315300	AT5G39160.3	61.644	219	75	3	1	215	1	214	2.28e-87	257

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Find 69 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTCTTGGAGAGGGCTG+GGG	0.564168	2_4:+11521113	None:intergenic
AAGTCCGAATAGTTGGTCTA+CGG	0.617492	2_4:+11520720	None:intergenic
AATAGTTGGTCTACGGTGAC+TGG	0.448660	2_4:+11520727	None:intergenic
AATTTGCAGTATTTGTGAAT+GGG	0.515890	2_4:-11520846	Msa0315300:intron
ACAAAAGTCTTGGAGAGGGC+TGG	0.468953	2_4:+11521111	None:intergenic
ACAATAATAATAAGTAGAGA+GGG	0.558687	2_4:+11520229	None:intergenic
ACCCACCTCACATTCACCCT+AGG	0.594952	2_4:-11520645	Msa0315300:CDS
AGAACCTCGTCGGAAATAAG+CGG	0.593140	2_4:+11520385	None:intergenic
AGCTTTGGTAAGAACCTCGT+CGG	0.612864	2_4:+11520375	None:intergenic
AGGGCTGGGGTCATAAGCAA+AGG	0.629845	2_4:+11521126	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATATACATACCACCATCTTT+GGG	+	chr2_4:11520245-11520264	None:intergenic	30.0%
TATATACATACCACCATCTT+TGG	+	chr2_4:11520246-11520265	None:intergenic	30.0%
TTTCAATTGAATGTGGGATA+TGG	-	chr2_4:11520828-11520847	Msa0315300:CDS	30.0%
ATTATCTTCAAAAGCAGTCT+TGG	-	chr2_4:11520988-11521007	Msa0315300:intron	30.0%
ATATACATACCACCATCTTT+GGG	+	chr2_4:11520245-11520264	None:intergenic	30.0%
TATATACATACCACCATCTT+TGG	+	chr2_4:11520246-11520265	None:intergenic	30.0%
TTTCAATTGAATGTGGGATA+TGG	-	chr2_4:11520828-11520847	Msa0315300:CDS	30.0%
ATTATCTTCAAAAGCAGTCT+TGG	-	chr2_4:11520988-11521007	Msa0315300:intron	30.0%
ATACTTCCTTGTTAGCATCT+TGG	-	chr2_4:11520148-11520167	Msa0315300:three_prime_UTR	35.0%
TGATTGCAACACAAAAGTCT+TGG	+	chr2_4:11520217-11520236	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	11520111	11521226	11520111	ID=Msa0315300;Name=Msa0315300
chr2_4	mRNA	11520111	11521226	11520111	ID=Msa0315300-mRNA-1;Parent=Msa0315300;Name=Msa0315300-mRNA-1;_AED=0.00;_eAED=0.00;_QI=26\|1\|1\|1\|1\|1\|2\|201\|221
chr2_4	exon	11520111	11520859	11520111	ID=Msa0315300-mRNA-1:exon:4435;Parent=Msa0315300-mRNA-1
chr2_4	exon	11521083	11521226	11521083	ID=Msa0315300-mRNA-1:exon:4434;Parent=Msa0315300-mRNA-1
chr2_4	five_prime_UTR	11521201	11521226	11521201	ID=Msa0315300-mRNA-1:five_prime_utr;Parent=Msa0315300-mRNA-1
chr2_4	CDS	11521083	11521200	11521083	ID=Msa0315300-mRNA-1:cds;Parent=Msa0315300-mRNA-1
chr2_4	CDS	11520312	11520859	11520312	ID=Msa0315300-mRNA-1:cds;Parent=Msa0315300-mRNA-1
chr2_4	three_prime_UTR	11520111	11520311	11520111	ID=Msa0315300-mRNA-1:three_prime_utr;Parent=Msa0315300-mRNA-1

Gene Sequence

>Msa0315300

ATGAAGATTCTATACTTCCTTGTTAGCATCTTGGCTTTGGCATCCTCTCTTGCCTTTGCTTATGACCCCAGCCCTCTCCAAGACTTTTGTGTTGCAATCAAAGACCCCAAAGATGGTGTATTTGTGAATGGGAAATTCTGTAAAGACCCCGAGCTTGTTAAAGCTGAAGATTTTTTCAAACATGTTGAAGCTGGGAATGCCTCTAACGCCTTGGGCTCTCAAGTGACTCCAGTCACCGTAGACCAACTATTCGGACTTAACACACTCGGTATATCTTTGGCTCGCATTGATTTTGTGCCTAAGGGTTTAAACCCACCTCACATTCACCCTAGGGGAACAGAGATCCTTATAGTACTCGAAGGCACTCTTTATGTTGGATTTGTCACGTCCAATCAAGACAACAATCGTCTCTTCACCAAAGTGCTAAACAAGGGTGATGTGTTTGTGTTCCCAATTGGTCTTATTCATTTTCAATTGAATGTGGGATATGGCAACGCTGTTGCTATTGCTGGACTTAGCAGTCAAAACCCAGGAGTTATCACTGTTGCAAATGCTTTGTTTAAATCTGACCCGCTTATTTCCGACGAGGTTCTTACCAAAGCTTTTCAAGTGGATAAGAGCATAATTGATTATCTTCAAAAGCAGTCTTGGTATGACAACAACTAG

Protein sequence

>Msa0315300

MKILYFLVSILALASSLAFAYDPSPLQDFCVAIKDPKDGVFVNGKFCKDPELVKAEDFFKHVEAGNASNALGSQVTPVTVDQLFGLNTLGISLARIDFVPKGLNPPHIHPRGTEILIVLEGTLYVGFVTSNQDNNRLFTKVLNKGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVITVANALFKSDPLISDEVLTKAFQVDKSIIDYLQKQSWYDNN*