AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90221

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90221.t1 MTR_4g093620 94.472 398 17 2 1 393 1 398 0.0 752
MS.gene90221.t1 MTR_7g066120 41.404 413 205 12 1 380 1 409 8.82e-92 282
MS.gene90221.t1 MTR_2g008030 42.122 311 155 7 82 378 32 331 1.25e-74 236
MS.gene90221.t1 MTR_6g035305 40.909 308 165 6 82 379 32 332 5.12e-73 231
MS.gene90221.t1 MTR_0776s0010 37.742 310 174 6 80 375 307 611 1.65e-56 200
MS.gene90221.t1 MTR_3g070100 26.148 283 170 12 119 386 120 378 1.41e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90221.t1 AT5G64380 76.389 360 74 3 40 393 50 404 0.0 561
MS.gene90221.t1 AT3G54050 46.417 321 152 6 79 380 96 415 4.80e-91 280
MS.gene90221.t1 AT3G54050 46.417 321 152 6 79 380 96 415 4.80e-91 280
MS.gene90221.t1 AT1G43670 41.956 317 167 7 82 388 32 341 1.39e-78 246
MS.gene90221.t1 AT3G55800 27.273 286 169 14 119 389 127 388 1.75e-14 74.7

Find 90 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTGCATTACCTTTGGCTC+TGG 0.272844 4.2:+19873920 MS.gene90221:CDS
TCCCTATCAGAACCACTATT+TGG 0.303466 4.2:-19872371 None:intergenic
AGTCTTTGGTGCAGTTTAAC+TGG 0.327999 4.2:-19874896 None:intergenic
ATTCACTCCAAAGTGGAAAT+AGG 0.331263 4.2:+19873855 MS.gene90221:CDS
CTCCATCGAACTTTGTTGTA+TGG 0.346740 4.2:+19874749 MS.gene90221:CDS
TACCTTGCAGAGTACTTCTT+AGG 0.348324 4.2:-19874704 None:intergenic
ATATATTTCCTTAAGCCTTC+AGG 0.350818 4.2:-19874650 None:intergenic
GTAAAACCACTATCAGTCTT+AGG 0.354058 4.2:+19872161 MS.gene90221:CDS
GTAGGTAAAGGAGGTGGAAT+TGG 0.362077 4.2:+19872248 MS.gene90221:CDS
GGTTGGAAAGTTTGAAGCTT+TGG 0.362731 4.2:-19872089 None:intergenic
TTGATTGGCCTGAAGGCTTA+AGG 0.371460 4.2:+19874642 MS.gene90221:CDS
CTTGTTACTGAAGCCATTGT+TGG 0.385235 4.2:-19872137 None:intergenic
AATTGGTGTGAATGATGATT+TGG 0.387215 4.2:+19872265 MS.gene90221:CDS
TTCACTCTCGACCGTTCAAC+AGG 0.404800 4.2:+19873953 MS.gene90221:CDS
ATGGAAGAGTTGGAAAGTTA+TGG 0.406247 4.2:+19874947 MS.gene90221:CDS
ACCATTGATATGCCAAATAG+TGG 0.413324 4.2:+19872359 MS.gene90221:CDS
CTATCAGCCTATTTCCACTT+TGG 0.418294 4.2:-19873862 None:intergenic
AAAAGTAAATCCTGGTTATG+AGG 0.419122 4.2:+19874982 MS.gene90221:CDS
GGATCTGTCACGACCACATA+TGG 0.420264 4.2:-19873719 None:intergenic
GAAGGTGATGAGTTGATAGA+AGG 0.422120 4.2:-19872063 None:intergenic
GATATAATATCAAGAGGCTT+TGG 0.431354 4.2:-19872398 None:intergenic
GGAAATAGGCTGATAGCTTC+TGG 0.433737 4.2:+19873869 MS.gene90221:CDS
TCCATCGAACTTTGTTGTAT+GGG 0.439545 4.2:+19874750 MS.gene90221:CDS
TGCCAAATAGTGGTTCTGAT+AGG 0.439936 4.2:+19872369 MS.gene90221:CDS
AAAGCCTCTTGATATTATAT+CGG 0.440217 4.2:+19872400 MS.gene90221:CDS
ACTATACTCTGCATTACCTT+TGG 0.441406 4.2:+19873914 MS.gene90221:CDS
TTTGAAAGACATGGAAGAGT+TGG 0.444010 4.2:+19874937 MS.gene90221:CDS
CCATTGTTGGAGTGAAGAAA+AGG 0.457793 4.2:-19872124 None:intergenic
ATCTGGAAAAGTTGCTGTCA+TGG 0.460073 4.2:+19873652 MS.gene90221:CDS
TCAACAGTAGCAAGTGATGA+TGG 0.462555 4.2:+19872203 MS.gene90221:CDS
CCATCGAACTTTGTTGTATG+GGG 0.479077 4.2:+19874751 MS.gene90221:CDS
ATTGATGCATCCATACCAAC+TGG 0.487257 4.2:+19873761 MS.gene90221:CDS
CTTCATAAACAAGACGAAGA+TGG 0.490062 4.2:-19874796 None:intergenic
TCTGCATTACCTTTGGCTCT+GGG 0.492545 4.2:+19873921 MS.gene90221:CDS
GAAAATTGTACCAGTTGGTA+TGG 0.498000 4.2:-19873771 None:intergenic
GCCAAATAGTGGTTCTGATA+GGG 0.510565 4.2:+19872370 MS.gene90221:CDS
GTGGAGCAAGCTGGTGGGAG+AGG 0.519722 4.2:+19874839 MS.gene90221:CDS
AGTTTGAAGCTTTGGTCGGA+AGG 0.522108 4.2:-19872081 None:intergenic
TGATGGGTCTGTTACATTGA+TGG 0.523388 4.2:+19872220 MS.gene90221:CDS
GAAGTACCTAAGACTGATAG+TGG 0.526082 4.2:-19872167 None:intergenic
ACCACTATTTGGCATATCAA+TGG 0.526712 4.2:-19872360 None:intergenic
TAATCTGCCCACAGAGGAGA+AGG 0.532639 4.2:+19873823 MS.gene90221:CDS
TGCTCCACGATGAAACTAAG+AGG 0.534585 4.2:-19874824 None:intergenic
TTGATGGAGTATGTAGGTAA+AGG 0.537527 4.2:+19872236 MS.gene90221:CDS
ATGGAGTATGTAGGTAAAGG+AGG 0.539783 4.2:+19872239 MS.gene90221:CDS
AGCAAAGCCTTCTCCTCTGT+GGG 0.541176 4.2:-19873830 None:intergenic
GAGTATGTAGGTAAAGGAGG+TGG 0.541348 4.2:+19872242 MS.gene90221:CDS
CCAACTTGGATAAGTGATGA+TGG 0.545731 4.2:+19873695 MS.gene90221:CDS
CATTGTTGGAGTGAAGAAAA+GGG 0.551735 4.2:-19872123 None:intergenic
GTTGGAGTGAAGAAAAGGGT+TGG 0.561218 4.2:-19872119 None:intergenic
CGGTATTTATAAACGCCTTG+AGG 0.565322 4.2:+19873793 MS.gene90221:CDS
AGTTTCATCGTGGAGCAAGC+TGG 0.566038 4.2:+19874830 MS.gene90221:CDS
CCATCATCACTTATCCAAGT+TGG 0.566146 4.2:-19873695 None:intergenic
AAGAAAATGATACACCAACT+TGG 0.571987 4.2:+19873681 MS.gene90221:CDS
GGGGTGTGGCGATGAATCCG+AGG 0.573022 4.2:+19874771 MS.gene90221:CDS
CAACAGTAGCAAGTGATGAT+GGG 0.579069 4.2:+19872204 MS.gene90221:CDS
GATACTGTGAGACAAGGAAA+AGG 0.580838 4.2:+19874674 MS.gene90221:CDS
TTGCTGAATTCACTCCAAAG+TGG 0.581831 4.2:+19873848 MS.gene90221:CDS
TTTAGGGAGTTTGAAAGACA+TGG 0.583051 4.2:+19874928 MS.gene90221:CDS
GTTACATTGATGGAGTATGT+AGG 0.584657 4.2:+19872230 MS.gene90221:CDS
CAGCAAAGCCTTCTCCTCTG+TGG 0.586371 4.2:-19873831 None:intergenic
TGCATGTGTCCCAGAGCCAA+AGG 0.587943 4.2:-19873930 None:intergenic
CCCCATACAACAAAGTTCGA+TGG 0.588449 4.2:-19874751 None:intergenic
ATATCAATGGTATAATTGAA+AGG 0.591706 4.2:-19872347 None:intergenic
TTCATCGTGGAGCAAGCTGG+TGG 0.592470 4.2:+19874833 MS.gene90221:CDS
GGAAAGTTTGAAGCTTTGGT+CGG 0.593641 4.2:-19872085 None:intergenic
AAGGTGATGAGTTGATAGAA+GGG 0.593820 4.2:-19872062 None:intergenic
ATCCTAAGAAGTACTCTGCA+AGG 0.594963 4.2:+19874702 MS.gene90221:CDS
GTCGTGACAGATCCCCTCGA+TGG 0.599505 4.2:+19873728 MS.gene90221:CDS
ATACCGAAAATTGTACCAGT+TGG 0.603945 4.2:-19873776 None:intergenic
CATCGAACTTTGTTGTATGG+GGG 0.606647 4.2:+19874752 MS.gene90221:CDS
TGCTACTGTTGATGAAGATG+AGG 0.607320 4.2:-19872192 None:intergenic
CACACCGATATAATATCAAG+AGG 0.613129 4.2:-19872404 None:intergenic
GGGTGTGGCGATGAATCCGA+GGG 0.615240 4.2:+19874772 MS.gene90221:CDS
TAGCATCAAACCTCATAACC+AGG 0.619050 4.2:-19874992 None:intergenic
GCAGATTATCTAGCTCCTCA+AGG 0.619596 4.2:-19873808 None:intergenic
AATATTTCGAGAACCATCGA+GGG 0.619869 4.2:-19873741 None:intergenic
ACAAGACGAAGATGGTCCCT+CGG 0.621507 4.2:-19874788 None:intergenic
GCTCCACGATGAAACTAAGA+GGG 0.623232 4.2:-19874823 None:intergenic
GAATGAAGTCTCCTGTTGAA+CGG 0.631390 4.2:-19873964 None:intergenic
GCTGGTGGGAGAGGATCCGA+TGG 0.631525 4.2:+19874848 MS.gene90221:CDS
AAGTGATGATGGTCCATATG+TGG 0.632479 4.2:+19873706 MS.gene90221:CDS
TCATCGTGGAGCAAGCTGGT+GGG 0.634727 4.2:+19874834 MS.gene90221:CDS
CAATATTTCGAGAACCATCG+AGG 0.656931 4.2:-19873742 None:intergenic
AGCATTAAAATTCCTCCCCG+TGG 0.680772 4.2:+19873998 MS.gene90221:CDS
TATATTGATACTGTGAGACA+AGG 0.681648 4.2:+19874668 MS.gene90221:CDS
GCTAGATAATCTGCCCACAG+AGG 0.689544 4.2:+19873817 MS.gene90221:CDS
GTGCATATGTTACCACGGGG+AGG 0.701876 4.2:-19874010 None:intergenic
ATATTTCGAGAACCATCGAG+GGG 0.721395 4.2:-19873740 None:intergenic
AAACCCTCTTAGTTTCATCG+TGG 0.730924 4.2:+19874820 MS.gene90221:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAAATATCTATCAAATT+AGG - chr4.2:19874084-19874103 None:intergenic 10.0%
!!! CTATACAATATTTTTATATT+TGG - chr4.2:19872931-19872950 None:intergenic 10.0%
!! ACATTAATGCTTATAATTTA+AGG + chr4.2:19874202-19874221 MS.gene90221:intron 15.0%
!! TATAAAAATAAACTGAAAAC+AGG + chr4.2:19874400-19874419 MS.gene90221:intron 15.0%
!! TTTCTATTAGGTTTAAATAT+CGG - chr4.2:19873028-19873047 None:intergenic 15.0%
!!! ATAAAAAACACTTTAAAAGT+CGG + chr4.2:19872739-19872758 MS.gene90221:intron 15.0%
!! AACTCAATATAGCATAAATT+AGG + chr4.2:19872715-19872734 MS.gene90221:intron 20.0%
!! ATATCAATGGTATAATTGAA+AGG - chr4.2:19872350-19872369 None:intergenic 20.0%
!! GTTCTATTAATGTTGTTAAA+TGG + chr4.2:19873379-19873398 MS.gene90221:intron 20.0%
!! TAACAAAACCCTTTATTAAA+AGG + chr4.2:19873552-19873571 MS.gene90221:intron 20.0%
!! TATTAGAAAATAAGTCATCT+AGG + chr4.2:19872881-19872900 MS.gene90221:intron 20.0%
!! TCTGTTATATTATCAAATGT+TGG + chr4.2:19874114-19874133 MS.gene90221:intron 20.0%
!! TTAAGCTATTTATCAAGAAA+GGG - chr4.2:19874228-19874247 None:intergenic 20.0%
!! TTCTATTAGGTTTAAATATC+GGG - chr4.2:19873027-19873046 None:intergenic 20.0%
!!! CTCTTTTTCCTTTTAATAAA+GGG - chr4.2:19873563-19873582 None:intergenic 20.0%
!!! TCTCTTTTTCCTTTTAATAA+AGG - chr4.2:19873564-19873583 None:intergenic 20.0%
!!! TTCAAAATTTTGAGAATTGA+GGG + chr4.2:19873099-19873118 MS.gene90221:intron 20.0%
!!! TTGATAATATAACAGAAGAT+AGG - chr4.2:19874110-19874129 None:intergenic 20.0%
!!! TTTATTTTTATAAGCTCTTC+AGG - chr4.2:19874392-19874411 None:intergenic 20.0%
! AAAAAAATCATCCAAACTCA+TGG - chr4.2:19872450-19872469 None:intergenic 25.0%
! AAAGCCTCTTGATATTATAT+CGG + chr4.2:19872400-19872419 MS.gene90221:CDS 25.0%
! AATAGAACAGTTTGTCATAA+TGG - chr4.2:19873367-19873386 None:intergenic 25.0%
! GTAAGTTATATGAAAACACA+AGG - chr4.2:19873060-19873079 None:intergenic 25.0%
! GTTAAGCTATTTATCAAGAA+AGG - chr4.2:19874229-19874248 None:intergenic 25.0%
! GTTCTACAACTTAGATATAA+GGG - chr4.2:19873197-19873216 None:intergenic 25.0%
! TGTTCTACAACTTAGATATA+AGG - chr4.2:19873198-19873217 None:intergenic 25.0%
! TTCTACAACTTAGATATAAG+GGG - chr4.2:19873196-19873215 None:intergenic 25.0%
!! GTTTGTCATAATGGAAATAT+AGG - chr4.2:19873358-19873377 None:intergenic 25.0%
!! TTACATTAATTTTCGATGAG+TGG - chr4.2:19873169-19873188 None:intergenic 25.0%
!! TTTGTGTGTTGTTGTTAATT+GGG + chr4.2:19872519-19872538 MS.gene90221:intron 25.0%
!! TTTTGATCTTATGTCAGAAT+TGG + chr4.2:19873418-19873437 MS.gene90221:intron 25.0%
!!! AATGTTGTTAAATGGATTGT+TGG + chr4.2:19873387-19873406 MS.gene90221:intron 25.0%
!!! ATGATGCAAGATATTTTGAT+TGG + chr4.2:19874627-19874646 MS.gene90221:CDS 25.0%
!!! ATGTTGTTAAATGGATTGTT+GGG + chr4.2:19873388-19873407 MS.gene90221:intron 25.0%
!!! CTTCAAAATTTTGAGAATTG+AGG + chr4.2:19873098-19873117 MS.gene90221:intron 25.0%
!!! TCAGTAATGGGTTTAAAAAT+AGG - chr4.2:19873445-19873464 None:intergenic 25.0%
AAAAGTAAATCCTGGTTATG+AGG + chr4.2:19874982-19875001 MS.gene90221:CDS 30.0%
AAACTGTTTGGTTGAGTTTA+TGG - chr4.2:19874487-19874506 None:intergenic 30.0%
AAATCGGTGGTTGAAAATAT+TGG + chr4.2:19872552-19872571 MS.gene90221:intron 30.0%
AAATTACCTTTGTAACAAGC+TGG + chr4.2:19872823-19872842 MS.gene90221:intron 30.0%
AAGAAAATGATACACCAACT+TGG + chr4.2:19873681-19873700 MS.gene90221:CDS 30.0%
AATAAACTGAAAACAGGCTA+TGG + chr4.2:19874406-19874425 MS.gene90221:intron 30.0%
AATCGGTGGTTGAAAATATT+GGG + chr4.2:19872553-19872572 MS.gene90221:intron 30.0%
AATTGGTGTGAATGATGATT+TGG + chr4.2:19872265-19872284 MS.gene90221:CDS 30.0%
ACTAACCAAGACAATCAAAA+AGG - chr4.2:19872477-19872496 None:intergenic 30.0%
AGAAAATAAGTCATCTAGGT+CGG + chr4.2:19872885-19872904 MS.gene90221:intron 30.0%
ATATATTTCCTTAAGCCTTC+AGG - chr4.2:19874653-19874672 None:intergenic 30.0%
ATTGGGAATCATGACAAAAT+CGG + chr4.2:19872536-19872555 MS.gene90221:intron 30.0%
CAGTCAACAACATAAACTTA+GGG - chr4.2:19874568-19874587 None:intergenic 30.0%
CTAACCAAGACAATCAAAAA+GGG - chr4.2:19872476-19872495 None:intergenic 30.0%
CTAGCCATTATGTATGTAAA+AGG + chr4.2:19873511-19873530 MS.gene90221:intron 30.0%
CTCTAACATAAGAGTTTCTA+AGG - chr4.2:19873271-19873290 None:intergenic 30.0%
CTCTTAAGAAGAAGAATAAG+TGG + chr4.2:19872623-19872642 MS.gene90221:intron 30.0%
GATATAATATCAAGAGGCTT+TGG - chr4.2:19872401-19872420 None:intergenic 30.0%
GTACAACAAAAAGTAAATCC+TGG + chr4.2:19874974-19874993 MS.gene90221:CDS 30.0%
TAGTATGGTACATCAGTAAT+GGG - chr4.2:19873457-19873476 None:intergenic 30.0%
TATATTGATACTGTGAGACA+AGG + chr4.2:19874668-19874687 MS.gene90221:CDS 30.0%
TCTATTGAATTCCATGAGTT+TGG + chr4.2:19872436-19872455 MS.gene90221:intron 30.0%
TTAAAGTGCATATGTTACCA+CGG - chr4.2:19874018-19874037 None:intergenic 30.0%
TTGTCATCACTTCAAAAATC+TGG + chr4.2:19873635-19873654 MS.gene90221:CDS 30.0%
TTGTTACAAAGGTAATTTGG+TGG - chr4.2:19872821-19872840 None:intergenic 30.0%
TTTCATTCTGCAATAATGCA+TGG - chr4.2:19873610-19873629 None:intergenic 30.0%
TTTCCACTGAAAACAACTTA+TGG + chr4.2:19874443-19874462 MS.gene90221:intron 30.0%
! AAAGACTTCCTCTCTTTTTA+GGG + chr4.2:19874912-19874931 MS.gene90221:CDS 30.0%
! AAGAAAAGGGTTGGAATTTT+TGG - chr4.2:19872113-19872132 None:intergenic 30.0%
! ACAGCCTTTTACATACATAA+TGG - chr4.2:19873518-19873537 None:intergenic 30.0%
! AGGATGACTCATTTTCTATT+AGG - chr4.2:19873040-19873059 None:intergenic 30.0%
! ATACCAACTGGTACAATTTT+CGG + chr4.2:19873773-19873792 MS.gene90221:CDS 30.0%
! CAAGGCTAATTTTCTTTCAT+TGG + chr4.2:19874149-19874168 MS.gene90221:intron 30.0%
! GTTTGTGTGTTGTTGTTAAT+TGG + chr4.2:19872518-19872537 MS.gene90221:intron 30.0%
!! AGCTTGTTACAAAGGTAATT+TGG - chr4.2:19872824-19872843 None:intergenic 30.0%
!! TCAAAATTTTGAAGACCCTT+GGG - chr4.2:19873092-19873111 None:intergenic 30.0%
!! TTTTCCCTTTTTGATTGTCT+TGG + chr4.2:19872469-19872488 MS.gene90221:intron 30.0%
!!! AAGAATCCTACTTTTTCCAT+CGG - chr4.2:19874867-19874886 None:intergenic 30.0%
AATATTTCGAGAACCATCGA+GGG - chr4.2:19873744-19873763 None:intergenic 35.0%
ACCACTATTTGGCATATCAA+TGG - chr4.2:19872363-19872382 None:intergenic 35.0%
ACCATTGATATGCCAAATAG+TGG + chr4.2:19872359-19872378 MS.gene90221:CDS 35.0%
ACTATACTCTGCATTACCTT+TGG + chr4.2:19873914-19873933 MS.gene90221:CDS 35.0%
ACTGATGCACAGATAGAAAT+AGG - chr4.2:19873142-19873161 None:intergenic 35.0%
ATACCGAAAATTGTACCAGT+TGG - chr4.2:19873779-19873798 None:intergenic 35.0%
ATGGAAGAGTTGGAAAGTTA+TGG + chr4.2:19874947-19874966 MS.gene90221:CDS 35.0%
ATTCACTCCAAAGTGGAAAT+AGG + chr4.2:19873855-19873874 MS.gene90221:CDS 35.0%
ATTCTGCAATAATGCATGGT+GGG - chr4.2:19873606-19873625 None:intergenic 35.0%
CACACCGATATAATATCAAG+AGG - chr4.2:19872407-19872426 None:intergenic 35.0%
CATAAACTTAGGGACTGTAT+AGG - chr4.2:19874558-19874577 None:intergenic 35.0%
CATTGTTGGAGTGAAGAAAA+GGG - chr4.2:19872126-19872145 None:intergenic 35.0%
CTAAAAAGAGAGGAAGTCTT+TGG - chr4.2:19874913-19874932 None:intergenic 35.0%
CTAGTATGGTACATCAGTAA+TGG - chr4.2:19873458-19873477 None:intergenic 35.0%
CTCACAAATGTTCATGTCTA+CGG + chr4.2:19874512-19874531 MS.gene90221:intron 35.0%
CTTATGTTAGAGTGTGTTGA+AGG + chr4.2:19873279-19873298 MS.gene90221:intron 35.0%
CTTCATAAACAAGACGAAGA+TGG - chr4.2:19874799-19874818 None:intergenic 35.0%
GCAGTCAACAACATAAACTT+AGG - chr4.2:19874569-19874588 None:intergenic 35.0%
GTAAAACCACTATCAGTCTT+AGG + chr4.2:19872161-19872180 MS.gene90221:CDS 35.0%
GTCAACATTCAAGACTAGTA+TGG - chr4.2:19873472-19873491 None:intergenic 35.0%
GTTACATTGATGGAGTATGT+AGG + chr4.2:19872230-19872249 MS.gene90221:CDS 35.0%
TAAAGTGCATATGTTACCAC+GGG - chr4.2:19874017-19874036 None:intergenic 35.0%
TGGCCTAATTCAACTATACA+AGG - chr4.2:19872911-19872930 None:intergenic 35.0%
TGGGTAGGAACATATATACA+GGG - chr4.2:19872671-19872690 None:intergenic 35.0%
TTCAAACTCCCTAAAAAGAG+AGG - chr4.2:19874923-19874942 None:intergenic 35.0%
TTGATGGAGTATGTAGGTAA+AGG + chr4.2:19872236-19872255 MS.gene90221:CDS 35.0%
TTGTGAGTTGTGAAACTGTT+TGG - chr4.2:19874499-19874518 None:intergenic 35.0%
TTTAGGGAGTTTGAAAGACA+TGG + chr4.2:19874928-19874947 MS.gene90221:CDS 35.0%
TTTGAAAGACATGGAAGAGT+TGG + chr4.2:19874937-19874956 MS.gene90221:CDS 35.0%
! CAAAGACTTCCTCTCTTTTT+AGG + chr4.2:19874911-19874930 MS.gene90221:CDS 35.0%
! GAAAATTGTACCAGTTGGTA+TGG - chr4.2:19873774-19873793 None:intergenic 35.0%
! TCCATCGAACTTTGTTGTAT+GGG + chr4.2:19874750-19874769 MS.gene90221:CDS 35.0%
! TTTTTGTCAAGTAGCCACAA+TGG + chr4.2:19872591-19872610 MS.gene90221:intron 35.0%
!! AAGAGAATTTCTAGCCATTG+TGG - chr4.2:19872608-19872627 None:intergenic 35.0%
!! AAGGTGATGAGTTGATAGAA+GGG - chr4.2:19872065-19872084 None:intergenic 35.0%
!! ACAAAACCAGCTTGTTACAA+AGG - chr4.2:19872832-19872851 None:intergenic 35.0%
!! AGTCCATAAGTTGTTTTCAG+TGG - chr4.2:19874449-19874468 None:intergenic 35.0%
!! CAAAATTTTGAAGACCCTTG+GGG - chr4.2:19873091-19873110 None:intergenic 35.0%
!! CTCAAAATTTTGAAGACCCT+TGG - chr4.2:19873093-19873112 None:intergenic 35.0%
!!! AAAGGGTTGGAATTTTTGGT+TGG - chr4.2:19872109-19872128 None:intergenic 35.0%
!!! AGATATTTTGATTGGCCTGA+AGG + chr4.2:19874635-19874654 MS.gene90221:CDS 35.0%
AAACCCTCTTAGTTTCATCG+TGG + chr4.2:19874820-19874839 MS.gene90221:CDS 40.0%
AAAGTGCATATGTTACCACG+GGG - chr4.2:19874016-19874035 None:intergenic 40.0%
AACTTACCAATTAAGCCCCA+AGG + chr4.2:19873073-19873092 MS.gene90221:intron 40.0%
AACTTAGGGACTGTATAGGA+TGG - chr4.2:19874554-19874573 None:intergenic 40.0%
ACTTACCAATTAAGCCCCAA+GGG + chr4.2:19873074-19873093 MS.gene90221:intron 40.0%
ACTTTGTTGGATGACTCGAA+TGG - chr4.2:19872965-19872984 None:intergenic 40.0%
AGAAGAATAAGTGGTGTGTC+AGG + chr4.2:19872632-19872651 MS.gene90221:intron 40.0%
AGTGACGAGTATCACTTTGT+TGG - chr4.2:19872978-19872997 None:intergenic 40.0%
ATACTCGTCACTCTACGAAT+TGG + chr4.2:19872986-19873005 MS.gene90221:intron 40.0%
ATATTTCGAGAACCATCGAG+GGG - chr4.2:19873743-19873762 None:intergenic 40.0%
ATCTGGAAAAGTTGCTGTCA+TGG + chr4.2:19873652-19873671 MS.gene90221:CDS 40.0%
ATGGAGTATGTAGGTAAAGG+AGG + chr4.2:19872239-19872258 MS.gene90221:CDS 40.0%
ATTGATGCATCCATACCAAC+TGG + chr4.2:19873761-19873780 MS.gene90221:CDS 40.0%
CAACAGTAGCAAGTGATGAT+GGG + chr4.2:19872204-19872223 MS.gene90221:CDS 40.0%
CAATATTTCGAGAACCATCG+AGG - chr4.2:19873745-19873764 None:intergenic 40.0%
CATTCTGCAATAATGCATGG+TGG - chr4.2:19873607-19873626 None:intergenic 40.0%
CCAACTTGGATAAGTGATGA+TGG + chr4.2:19873695-19873714 MS.gene90221:CDS 40.0%
CCATCATCACTTATCCAAGT+TGG - chr4.2:19873698-19873717 None:intergenic 40.0%
CCATTGTTGGAGTGAAGAAA+AGG - chr4.2:19872127-19872146 None:intergenic 40.0%
CGGTATTTATAAACGCCTTG+AGG + chr4.2:19873793-19873812 MS.gene90221:CDS 40.0%
CTATCAGCCTATTTCCACTT+TGG - chr4.2:19873865-19873884 None:intergenic 40.0%
CTTGTTACTGAAGCCATTGT+TGG - chr4.2:19872140-19872159 None:intergenic 40.0%
GAAGAATAAGTGGTGTGTCA+GGG + chr4.2:19872633-19872652 MS.gene90221:intron 40.0%
GAATGAAGTCTCCTGTTGAA+CGG - chr4.2:19873967-19873986 None:intergenic 40.0%
GATACTGTGAGACAAGGAAA+AGG + chr4.2:19874674-19874693 MS.gene90221:CDS 40.0%
GGGTAGGAACATATATACAG+GGG - chr4.2:19872670-19872689 None:intergenic 40.0%
GTGGGTAGGAACATATATAC+AGG - chr4.2:19872672-19872691 None:intergenic 40.0%
TCAACAGTAGCAAGTGATGA+TGG + chr4.2:19872203-19872222 MS.gene90221:CDS 40.0%
TCCCTATCAGAACCACTATT+TGG - chr4.2:19872374-19872393 None:intergenic 40.0%
TTCTGCAATAATGCATGGTG+GGG - chr4.2:19873605-19873624 None:intergenic 40.0%
TTGCTGAATTCACTCCAAAG+TGG + chr4.2:19873848-19873867 MS.gene90221:CDS 40.0%
! AGTCTTTGGTGCAGTTTAAC+TGG - chr4.2:19874899-19874918 None:intergenic 40.0%
! CATCGAACTTTGTTGTATGG+GGG + chr4.2:19874752-19874771 MS.gene90221:CDS 40.0%
! CCATCGAACTTTGTTGTATG+GGG + chr4.2:19874751-19874770 MS.gene90221:CDS 40.0%
! CCTTTTCTTCACTCCAACAA+TGG + chr4.2:19872124-19872143 MS.gene90221:CDS 40.0%
! CGGCCTTGTATAGTTGAATT+AGG + chr4.2:19872905-19872924 MS.gene90221:intron 40.0%
! CTCCATCGAACTTTGTTGTA+TGG + chr4.2:19874749-19874768 MS.gene90221:CDS 40.0%
! GAAGTACCTAAGACTGATAG+TGG - chr4.2:19872170-19872189 None:intergenic 40.0%
! GGAAAGTTTGAAGCTTTGGT+CGG - chr4.2:19872088-19872107 None:intergenic 40.0%
! GGTTGGAAAGTTTGAAGCTT+TGG - chr4.2:19872092-19872111 None:intergenic 40.0%
! TACCTTGCAGAGTACTTCTT+AGG - chr4.2:19874707-19874726 None:intergenic 40.0%
! TGATGGGTCTGTTACATTGA+TGG + chr4.2:19872220-19872239 MS.gene90221:CDS 40.0%
! TGCTACTGTTGATGAAGATG+AGG - chr4.2:19872195-19872214 None:intergenic 40.0%
!! AAGTGATGATGGTCCATATG+TGG + chr4.2:19873706-19873725 MS.gene90221:CDS 40.0%
!! ATCCTAAGAAGTACTCTGCA+AGG + chr4.2:19874702-19874721 MS.gene90221:CDS 40.0%
!! GAAGGTGATGAGTTGATAGA+AGG - chr4.2:19872066-19872085 None:intergenic 40.0%
!! GCCAAATAGTGGTTCTGATA+GGG + chr4.2:19872370-19872389 MS.gene90221:CDS 40.0%
!! TGCCAAATAGTGGTTCTGAT+AGG + chr4.2:19872369-19872388 MS.gene90221:CDS 40.0%
!!! ACAAGCTGGTTTTGTAGAGA+TGG + chr4.2:19872837-19872856 MS.gene90221:intron 40.0%
!!! CAAGCTGGTTTTGTAGAGAT+GGG + chr4.2:19872838-19872857 MS.gene90221:intron 40.0%
!!! CTCTCTTGTTTTGTCACTCA+GGG + chr4.2:19874588-19874607 MS.gene90221:intron 40.0%
AGCATTAAAATTCCTCCCCG+TGG + chr4.2:19873998-19874017 MS.gene90221:CDS 45.0%
CCCCATACAACAAAGTTCGA+TGG - chr4.2:19874754-19874773 None:intergenic 45.0%
CGTCACTCTACGAATTGGTT+TGG + chr4.2:19872991-19873010 MS.gene90221:intron 45.0%
GAGGATCCGATGGAAAAAGT+AGG + chr4.2:19874858-19874877 MS.gene90221:CDS 45.0%
GAGTATGTAGGTAAAGGAGG+TGG + chr4.2:19872242-19872261 MS.gene90221:CDS 45.0%
GCTCCACGATGAAACTAAGA+GGG - chr4.2:19874826-19874845 None:intergenic 45.0%
GGAAATAGGCTGATAGCTTC+TGG + chr4.2:19873869-19873888 MS.gene90221:CDS 45.0%
GGGAATCATGACAAAATCGG+TGG + chr4.2:19872539-19872558 MS.gene90221:intron 45.0%
GTAGGTAAAGGAGGTGGAAT+TGG + chr4.2:19872248-19872267 MS.gene90221:CDS 45.0%
GTTGGAGTGAAGAAAAGGGT+TGG - chr4.2:19872122-19872141 None:intergenic 45.0%
TGCTCCACGATGAAACTAAG+AGG - chr4.2:19874827-19874846 None:intergenic 45.0%
! AACTTTGTTGTATGGGGGTG+TGG + chr4.2:19874757-19874776 MS.gene90221:CDS 45.0%
! AGTTTGAAGCTTTGGTCGGA+AGG - chr4.2:19872084-19872103 None:intergenic 45.0%
! GCAGATTATCTAGCTCCTCA+AGG - chr4.2:19873811-19873830 None:intergenic 45.0%
!! CGGGGAGGAATTTTAATGCT+TGG - chr4.2:19873998-19874017 None:intergenic 45.0%
!! TCTGCATTACCTTTGGCTCT+GGG + chr4.2:19873921-19873940 MS.gene90221:CDS 45.0%
!! TGTTGGAAGCATCTCACACA+AGG + chr4.2:19874131-19874150 MS.gene90221:intron 45.0%
!! TTGATTGGCCTGAAGGCTTA+AGG + chr4.2:19874642-19874661 MS.gene90221:CDS 45.0%
!!! GCTCTCTTGTTTTGTCACTC+AGG + chr4.2:19874587-19874606 MS.gene90221:intron 45.0%
ACAAGACGAAGATGGTCCCT+CGG - chr4.2:19874791-19874810 None:intergenic 50.0%
AGCAAAGCCTTCTCCTCTGT+GGG - chr4.2:19873833-19873852 None:intergenic 50.0%
AGTTTCATCGTGGAGCAAGC+TGG + chr4.2:19874830-19874849 MS.gene90221:CDS 50.0%
GAAGACCCTTGGGGCTTAAT+TGG - chr4.2:19873082-19873101 None:intergenic 50.0%
GCTAGATAATCTGCCCACAG+AGG + chr4.2:19873817-19873836 MS.gene90221:CDS 50.0%
GGATCTGTCACGACCACATA+TGG - chr4.2:19873722-19873741 None:intergenic 50.0%
TAATCTGCCCACAGAGGAGA+AGG + chr4.2:19873823-19873842 MS.gene90221:CDS 50.0%
TACCCACTGAGCTAAGCTCA+CGG + chr4.2:19872685-19872704 MS.gene90221:intron 50.0%
TTCACTCTCGACCGTTCAAC+AGG + chr4.2:19873953-19873972 MS.gene90221:CDS 50.0%
! CTCTGCATTACCTTTGGCTC+TGG + chr4.2:19873920-19873939 MS.gene90221:CDS 50.0%
ACCCACTGAGCTAAGCTCAC+GGG + chr4.2:19872686-19872705 MS.gene90221:intron 55.0%
CAGCAAAGCCTTCTCCTCTG+TGG - chr4.2:19873834-19873853 None:intergenic 55.0%
GGTGTGTCAGGGTTCGAACT+CGG + chr4.2:19872644-19872663 MS.gene90221:intron 55.0%
GTGAGCTTAGCTCAGTGGGT+AGG - chr4.2:19872686-19872705 None:intergenic 55.0%
GTGCATATGTTACCACGGGG+AGG - chr4.2:19874013-19874032 None:intergenic 55.0%
TCATCGTGGAGCAAGCTGGT+GGG + chr4.2:19874834-19874853 MS.gene90221:CDS 55.0%
TGCATGTGTCCCAGAGCCAA+AGG - chr4.2:19873933-19873952 None:intergenic 55.0%
TTCATCGTGGAGCAAGCTGG+TGG + chr4.2:19874833-19874852 MS.gene90221:CDS 55.0%
CCCACTGAGCTAAGCTCACG+GGG + chr4.2:19872687-19872706 MS.gene90221:intron 60.0%
CCCCGTGAGCTTAGCTCAGT+GGG - chr4.2:19872690-19872709 None:intergenic 60.0%
GGGTGTGGCGATGAATCCGA+GGG + chr4.2:19874772-19874791 MS.gene90221:CDS 60.0%
GTCGTGACAGATCCCCTCGA+TGG + chr4.2:19873728-19873747 MS.gene90221:CDS 60.0%
TCCCCGTGAGCTTAGCTCAG+TGG - chr4.2:19872691-19872710 None:intergenic 60.0%
GCTGGTGGGAGAGGATCCGA+TGG + chr4.2:19874848-19874867 MS.gene90221:CDS 65.0%
GGGGTGTGGCGATGAATCCG+AGG + chr4.2:19874771-19874790 MS.gene90221:CDS 65.0%
GTGGAGCAAGCTGGTGGGAG+AGG + chr4.2:19874839-19874858 MS.gene90221:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 19872038 19875009 19872038 ID=MS.gene90221
chr4.2 mRNA 19872038 19875009 19872038 ID=MS.gene90221.t1;Parent=MS.gene90221
chr4.2 exon 19872038 19872421 19872038 ID=MS.gene90221.t1.exon1;Parent=MS.gene90221.t1
chr4.2 CDS 19872038 19872421 19872038 ID=cds.MS.gene90221.t1;Parent=MS.gene90221.t1
chr4.2 exon 19873623 19874019 19873623 ID=MS.gene90221.t1.exon2;Parent=MS.gene90221.t1
chr4.2 CDS 19873623 19874019 19873623 ID=cds.MS.gene90221.t1;Parent=MS.gene90221.t1
chr4.2 exon 19874609 19875009 19874609 ID=MS.gene90221.t1.exon3;Parent=MS.gene90221.t1
chr4.2 CDS 19874609 19875009 19874609 ID=cds.MS.gene90221.t1;Parent=MS.gene90221.t1
Gene Sequence

>MS.gene90221

ATGCAGTCAGCAGCAACAACAACACCCTTCTATCAACTCATCACCTTCCGACCAAAGCTTCAAACTTTCCAACCAAAAATTCCAACCCTTTTCTTCACTCCAACAATGGCTTCAGTAACAAGAGTAAAACCACTATCAGTCTTAGGTACTTCTTCCTCATCTTCATCAACAGTAGCAAGTGATGATGGGTCTGTTACATTGATGGAGTATGTAGGTAAAGGAGGTGGAATTGGTGTGAATGATGATTTGGTGATTTTGATTGATCATATACAGTATGCTTGTAAGAGAATTGCAGCTCTTATAGCTTCTCCTTTCAATTATACCATTGATATGCCAAATAGTGGTTCTGATAGGGATGCTCCAAAGCCTCTTGATATTATATCGGTGTGTTTTTCTTCTCTATTGAATTCCATGAGTTTGGATGATTTTTTTTTTCCCTTTTTGATTGTCTTGGTTAGTTTTGTCATGTTTTTTCGTTGTGTTTGTGTGTTGTTGTTAATTGGGAATCATGACAAAATCGGTGGTTGAAAATATTGGGAAAAACTTTTTTTTTTTTTTGTCAAGTAGCCACAATGGCTAGAAATTCTCTTAAGAAGAAGAATAAGTGGTGTGTCAGGGTTCGAACTCGGCCCCTGTATATATGTTCCTACCCACTGAGCTAAGCTCACGGGGACATTAACTCAATATAGCATAAATTAGGTATAAAAAACACTTTAAAAGTCGGTTTTGTAAGCATGAATTAGATCAACTATAGCATTAGAGTCTATCTATTCATGTCATCCACCAAATTACCTTTGTAACAAGCTGGTTTTGTAGAGATGGGTTAAATCTGATACTAAAACTTATTAGAAAATAAGTCATCTAGGTCGGCCTTGTATAGTTGAATTAGGCCAAATATAAAAATATTGTATAGCGTTAGTCTATCCATTCGAGTCATCCAACAAAGTGATACTCGTCACTCTACGAATTGGTTTGGATTAGTTAGACCCGATATTTAAACCTAATAGAAAATGAGTCATCCTTGTGTTTTCATATAACTTACCAATTAAGCCCCAAGGGTCTTCAAAATTTTGAGAATTGAGGGTTTCATATCATTTCATGCCTATTTCTATCTGTGCATCAGTTGAACCACTCATCGAAAATTAATGTAAGCTACCCCTTATATCTAAGTTGTAGAACATCATCATGAGAATTTCTTGACTGCTGATAGTGATATATCTACTTATAAATCCTTAGAAACTCTTATGTTAGAGTGTGTTGAAGGTTATATATTCTTGACTCAAGTCTATGTATGTAAGTCTTGCATGAGATATATAACCTATATTTCCATTATGACAAACTGTTCTATTAATGTTGTTAAATGGATTGTTGGGCTAATAATTTTGATCTTATGTCAGAATTGGTCCTATTTTTAAACCCATTACTGATGTACCATACTAGTCTTGAATGTTGACTGTCATATCTAAATTCTTTCTAGCCATTATGTATGTAAAAGGCTGTGTAATTTACTTTCTTAACAAAACCCTTTATTAAAAGGAAAAAGAGAATCTGAGTCATCAATTATCCCCACCATGCATTATTGCAGAATGAAATTATCTTGTCATCACTTCAAAAATCTGGAAAAGTTGCTGTCATGGCTTCTGAAGAAAATGATACACCAACTTGGATAAGTGATGATGGTCCATATGTGGTCGTGACAGATCCCCTCGATGGTTCTCGAAATATTGATGCATCCATACCAACTGGTACAATTTTCGGTATTTATAAACGCCTTGAGGAGCTAGATAATCTGCCCACAGAGGAGAAGGCTTTGCTGAATTCACTCCAAAGTGGAAATAGGCTGATAGCTTCTGGTTATGTTCTTTATTCATCTGCAACTATACTCTGCATTACCTTTGGCTCTGGGACACATGCATTCACTCTCGACCGTTCAACAGGAGACTTCATTCTGACAAATCCAAGCATTAAAATTCCTCCCCGTGGTAACATATGCACTTTAATTATGACATATACACAAATATTTACAGATCTAATATCAACTTTCCTAATTTGATAGATATTTTATAACTCCTATCTTCTGTTATATTATCAAATGTTGGAAGCATCTCACACAAGGCTAATTTTCTTTCATTGGTTATTTTCATTGTTTTCTTACTTCATGTAGACATTAATGCTTATAATTTAAGGCCCTTTCTTGATAAATAGCTTAACTTAGCACTTTAGCACAAGCGCTTATCATATAAATGCTTATGTTTAAGCTATGTCTATAGCAAAAGATAAAATAAAGTTAAACTATTTTCATATAAACTATAAATTGTTTTCATAAACTATAAATTGTTTTCATAAACTATCCTGAAGAGCTTATAAAAATAAACTGAAAACAGGCTATGGACATGTCAAAGTTGTTTCCACTGAAAACAACTTATGGACTTGTCAAAATTTGTTTCCATAAACTCAACCAAACAGTTTCACAACTCACAAATGTTCATGTCTACGGATAAGTTCAAATAAGTCCATCCTATACAGTCCCTAAGTTTATGTTGTTGACTGCTCTCTTGTTTTGTCACTCAGGGCAAATATATTCTGTGAATGATGCAAGATATTTTGATTGGCCTGAAGGCTTAAGGAAATATATTGATACTGTGAGACAAGGAAAAGGTAGATATCCTAAGAAGTACTCTGCAAGGTATATATGCTCTCTAGTAGCTGATCTCCATCGAACTTTGTTGTATGGGGGTGTGGCGATGAATCCGAGGGACCATCTTCGTCTTGTTTATGAAGCAAACCCTCTTAGTTTCATCGTGGAGCAAGCTGGTGGGAGAGGATCCGATGGAAAAAGTAGGATTCTTTCTCTTCAACCAGTTAAACTGCACCAAAGACTTCCTCTCTTTTTAGGGAGTTTGAAAGACATGGAAGAGTTGGAAAGTTATGGAGACGTACAACAAAAAGTAAATCCTGGTTATGAGGTTTGA
Protein sequence

>MS.gene90221.t1

MQSAATTTPFYQLITFRPKLQTFQPKIPTLFFTPTMASVTRVKPLSVLGTSSSSSSTVASDDGSVTLMEYVGKGGGIGVNDDLVILIDHIQYACKRIAALIASPFNYTIDMPNSGSDRDAPKPLDIISNEIILSSLQKSGKVAVMASEENDTPTWISDDGPYVVVTDPLDGSRNIDASIPTGTIFGIYKRLEELDNLPTEEKALLNSLQSGNRLIASGYVLYSSATILCITFGSGTHAFTLDRSTGDFILTNPSIKIPPRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGRYPKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYEANPLSFIVEQAGGRGSDGKSRILSLQPVKLHQRLPLFLGSLKDMEELESYGDVQQKVNPGYEV