Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008383.01.T01 | XP_013463516.1 | 100 | 114 | 0 | 0 | 21 | 134 | 1 | 114 | 4.50E-76 | 231 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008383.01.T01 | Q570U6 | 70.536 | 112 | 33 | 0 | 21 | 132 | 1 | 112 | 3.49E-50 | 158 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008383.01.T01 | A0A2Z6M7T0 | 100.000 | 114 | 0 | 0 | 21 | 134 | 1 | 114 | 2.15e-76 | 231 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048660.01 | MsG0280008383.01 | 0.807409 | 5.127834e-50 | 2.854015e-47 |
| MsG0180000129.01 | MsG0280008383.01 | 0.804353 | 2.249596e-49 | 1.157396e-46 |
| MsG0180000218.01 | MsG0280008383.01 | -0.835893 | 1.287409e-56 | 1.595893e-53 |
| MsG0180000241.01 | MsG0280008383.01 | 0.826450 | 2.694889e-54 | 2.528217e-51 |
| MsG0180000599.01 | MsG0280008383.01 | 0.804686 | 1.917899e-49 | 9.952150e-47 |
| MsG0180001610.01 | MsG0280008383.01 | 0.806815 | 6.847002e-50 | 3.752578e-47 |
| MsG0180002399.01 | MsG0280008383.01 | 0.802017 | 6.848967e-49 | 3.319140e-46 |
| MsG0180003353.01 | MsG0280008383.01 | 0.804149 | 2.480784e-49 | 1.269662e-46 |
| MsG0180003885.01 | MsG0280008383.01 | 0.834098 | 3.647838e-56 | 4.284984e-53 |
| MsG0180004390.01 | MsG0280008383.01 | 0.813225 | 2.850994e-51 | 1.850936e-48 |
| MsG0180004493.01 | MsG0280008383.01 | 0.822551 | 2.229679e-53 | 1.872390e-50 |
| MsG0180004500.01 | MsG0280008383.01 | 0.823874 | 1.094714e-53 | 9.543303e-51 |
| MsG0180004765.01 | MsG0280008383.01 | 0.820614 | 6.248564e-53 | 4.968137e-50 |
| MsG0180005153.01 | MsG0280008383.01 | 0.822943 | 1.806888e-53 | 1.534251e-50 |
| MsG0180005524.01 | MsG0280008383.01 | 0.806979 | 6.322388e-50 | 3.479706e-47 |
| MsG0180006017.01 | MsG0280008383.01 | 0.825791 | 3.865032e-54 | 3.558748e-51 |
| MsG0180006031.01 | MsG0280008383.01 | 0.808760 | 2.643476e-50 | 1.524198e-47 |
| MsG0180006035.01 | MsG0280008383.01 | 0.812331 | 4.474801e-51 | 2.837093e-48 |
| MsG0180006054.01 | MsG0280008383.01 | 0.817716 | 2.854446e-52 | 2.094653e-49 |
| MsG0280006570.01 | MsG0280008383.01 | 0.804106 | 2.532512e-49 | 1.294702e-46 |
| MsG0280006601.01 | MsG0280008383.01 | 0.826895 | 2.110811e-54 | 2.005699e-51 |
| MsG0280006711.01 | MsG0280008383.01 | -0.810982 | 8.792627e-51 | 5.378203e-48 |
| MsG0280007118.01 | MsG0280008383.01 | 0.833769 | 4.408543e-56 | 5.127762e-53 |
| MsG0280007307.01 | MsG0280008383.01 | 0.820607 | 6.274548e-53 | 4.987601e-50 |
| MsG0280007547.01 | MsG0280008383.01 | 0.801127 | 1.042609e-48 | 4.940391e-46 |
| MsG0280008112.01 | MsG0280008383.01 | 0.814630 | 1.396784e-51 | 9.421283e-49 |
| MsG0280008165.01 | MsG0280008383.01 | 0.802022 | 6.832730e-49 | 3.311675e-46 |
| MsG0280008171.01 | MsG0280008383.01 | 0.812580 | 3.947137e-51 | 2.519052e-48 |
| MsG0280008383.01 | MsG0280008391.01 | 0.802334 | 5.894967e-49 | 2.880070e-46 |
| MsG0280008383.01 | MsG0280009256.01 | 0.816582 | 5.134013e-52 | 3.652170e-49 |
| MsG0280008383.01 | MsG0280009756.01 | 0.823273 | 1.514086e-53 | 1.297574e-50 |
| MsG0280008383.01 | MsG0280010148.01 | 0.823643 | 1.240252e-53 | 1.074158e-50 |
| MsG0280008383.01 | MsG0280010349.01 | 0.802798 | 4.729131e-49 | 2.337966e-46 |
| MsG0280008383.01 | MsG0280010350.01 | 0.806764 | 7.021138e-50 | 3.842776e-47 |
| MsG0280008383.01 | MsG0280010517.01 | 0.814192 | 1.746485e-51 | 1.164062e-48 |
| MsG0280008383.01 | MsG0280010722.01 | 0.807463 | 4.993754e-50 | 2.783318e-47 |
| MsG0280008383.01 | MsG0280010916.01 | 0.842439 | 2.583907e-58 | 3.918409e-55 |
| MsG0280008383.01 | MsG0280011255.01 | 0.804663 | 1.938621e-49 | 1.005399e-46 |
| MsG0280008383.01 | MsG0280011272.01 | -0.859826 | 3.161166e-63 | 8.461983e-60 |
| MsG0280008383.01 | MsG0380012011.01 | 0.813556 | 2.410712e-51 | 1.579384e-48 |
| MsG0280008383.01 | MsG0380012567.01 | 0.818382 | 2.017930e-52 | 1.508310e-49 |
| MsG0280008383.01 | MsG0380013295.01 | -0.809525 | 1.812939e-50 | 1.066881e-47 |
| MsG0280008383.01 | MsG0380014103.01 | 0.801108 | 1.052092e-48 | 4.982974e-46 |
| MsG0280008383.01 | MsG0380014386.01 | 0.808137 | 3.589635e-50 | 2.036324e-47 |
| MsG0280008383.01 | MsG0380014706.01 | 0.814102 | 1.827377e-51 | 1.215010e-48 |
| MsG0280008383.01 | MsG0380014948.01 | 0.805538 | 1.271742e-49 | 6.745329e-47 |
| MsG0280008383.01 | MsG0380015131.01 | 0.810283 | 1.245004e-50 | 7.473730e-48 |
| MsG0280008383.01 | MsG0380015533.01 | 0.800207 | 1.605986e-48 | 7.434128e-46 |
| MsG0280008383.01 | MsG0380015784.01 | 0.810344 | 1.207516e-50 | 7.260603e-48 |
| MsG0280008383.01 | MsG0380015891.01 | 0.805870 | 1.083282e-49 | 5.795345e-47 |
| MsG0280008383.01 | MsG0380015896.01 | 0.814556 | 1.451063e-51 | 9.767763e-49 |
| MsG0280008383.01 | MsG0380016285.01 | 0.814610 | 1.411206e-51 | 9.513226e-49 |
| MsG0280008383.01 | MsG0380016347.01 | -0.818848 | 1.581949e-52 | 1.197717e-49 |
| MsG0280008383.01 | MsG0380016740.01 | 0.801955 | 7.052088e-49 | 3.412108e-46 |
| MsG0280008383.01 | MsG0380017094.01 | -0.800290 | 1.544637e-48 | 7.165475e-46 |
| MsG0280008383.01 | MsG0380017202.01 | 0.823690 | 1.209483e-53 | 1.048987e-50 |
| MsG0280008383.01 | MsG0380017293.01 | 0.812925 | 3.316960e-51 | 2.136113e-48 |
| MsG0280008383.01 | MsG0380017931.01 | 0.824595 | 7.414580e-54 | 6.598019e-51 |
| MsG0280008383.01 | MsG0380018075.01 | 0.818702 | 1.707528e-52 | 1.287448e-49 |
| MsG0280008383.01 | MsG0480018584.01 | 0.833321 | 5.704351e-56 | 6.546610e-53 |
| MsG0280008383.01 | MsG0480018653.01 | 0.806188 | 9.288804e-50 | 5.009817e-47 |
| MsG0280008383.01 | MsG0480018911.01 | 0.815766 | 7.813605e-52 | 5.436136e-49 |
| MsG0280008383.01 | MsG0480019206.01 | 0.803891 | 2.807730e-49 | 1.427554e-46 |
| MsG0280008383.01 | MsG0480019711.01 | 0.825240 | 5.222027e-54 | 4.733128e-51 |
| MsG0280008383.01 | MsG0480020024.01 | 0.815535 | 8.797336e-52 | 6.081545e-49 |
| MsG0280008383.01 | MsG0480020765.01 | 0.833171 | 6.216913e-56 | 7.102799e-53 |
| MsG0280008383.01 | MsG0480020788.01 | 0.822921 | 1.828711e-53 | 1.551820e-50 |
| MsG0280008383.01 | MsG0480020832.01 | 0.810692 | 1.016058e-50 | 6.166376e-48 |
| MsG0280008383.01 | MsG0480020887.01 | 0.813926 | 1.998338e-51 | 1.322348e-48 |
| MsG0280008383.01 | MsG0480020935.01 | -0.854749 | 1.000284e-61 | 2.256986e-58 |
| MsG0280008383.01 | MsG0480021060.01 | 0.817298 | 3.545083e-52 | 2.571795e-49 |
| MsG0280008383.01 | MsG0480021869.01 | 0.801130 | 1.040952e-48 | 4.932976e-46 |
| MsG0280008383.01 | MsG0480022491.01 | -0.833058 | 6.633639e-56 | 7.553380e-53 |
| MsG0280008383.01 | MsG0480022589.01 | 0.812556 | 3.994981e-51 | 2.548024e-48 |
| MsG0280008383.01 | MsG0480023073.01 | 0.815993 | 6.953575e-52 | 4.867609e-49 |
| MsG0280008383.01 | MsG0480023150.01 | 0.807155 | 5.803684e-50 | 3.208830e-47 |
| MsG0280008383.01 | MsG0480023251.01 | 0.848905 | 4.548611e-60 | 8.475667e-57 |
| MsG0280008383.01 | MsG0480023488.01 | 0.809881 | 1.519726e-50 | 9.028009e-48 |
| MsG0280008383.01 | MsG0480023489.01 | 0.821533 | 3.838457e-53 | 3.131034e-50 |
| MsG0280008383.01 | MsG0480023601.01 | 0.801279 | 9.706688e-49 | 4.617035e-46 |
| MsG0280008383.01 | MsG0580024790.01 | -0.867586 | 1.227419e-65 | 4.308065e-62 |
| MsG0280008383.01 | MsG0580025357.01 | -0.808736 | 2.674794e-50 | 1.541229e-47 |
| MsG0280008383.01 | MsG0580025407.01 | -0.853991 | 1.656808e-61 | 3.646439e-58 |
| MsG0280008383.01 | MsG0580025816.01 | 0.819804 | 9.582697e-53 | 7.450208e-50 |
| MsG0280008383.01 | MsG0580025987.01 | 0.838463 | 2.832810e-57 | 3.797268e-54 |
| MsG0280008383.01 | MsG0580027394.01 | 0.810717 | 1.003011e-50 | 6.091547e-48 |
| MsG0280008383.01 | MsG0580028428.01 | 0.829606 | 4.686403e-55 | 4.820026e-52 |
| MsG0280008383.01 | MsG0580029236.01 | 0.848110 | 7.552052e-60 | 1.371113e-56 |
| MsG0280008383.01 | MsG0580029376.01 | 0.802415 | 5.671949e-49 | 2.776893e-46 |
| MsG0280008383.01 | MsG0580029529.01 | 0.801658 | 8.115681e-49 | 3.897245e-46 |
| MsG0280008383.01 | MsG0580029611.01 | 0.829321 | 5.496546e-55 | 5.607238e-52 |
| MsG0280008383.01 | MsG0580029919.01 | 0.804992 | 1.655314e-49 | 8.657661e-47 |
| MsG0280008383.01 | MsG0580030000.01 | 0.807987 | 3.863507e-50 | 2.182991e-47 |
| MsG0280008383.01 | MsG0680030356.01 | -0.812477 | 4.157758e-51 | 2.646407e-48 |
| MsG0280008383.01 | MsG0680030880.01 | 0.810958 | 8.897187e-51 | 5.438607e-48 |
| MsG0280008383.01 | MsG0680031146.01 | 0.808155 | 3.558178e-50 | 2.019475e-47 |
| MsG0280008383.01 | MsG0680031702.01 | 0.802650 | 5.072570e-49 | 2.498189e-46 |
| MsG0280008383.01 | MsG0680032460.01 | -0.809003 | 2.345182e-50 | 1.360962e-47 |
| MsG0280008383.01 | MsG0680032771.01 | 0.800849 | 1.188504e-48 | 5.592098e-46 |
| MsG0280008383.01 | MsG0680033465.01 | 0.839694 | 1.359557e-57 | 1.892211e-54 |
| MsG0280008383.01 | MsG0680034230.01 | 0.824436 | 8.080439e-54 | 7.156658e-51 |
| MsG0280008383.01 | MsG0680034480.01 | 0.821997 | 2.997378e-53 | 2.477441e-50 |
| MsG0280008383.01 | MsG0680034601.01 | 0.874331 | 7.342874e-68 | 3.291955e-64 |
| MsG0280008383.01 | MsG0680034739.01 | 0.819626 | 1.052449e-52 | 8.141066e-50 |
| MsG0280008383.01 | MsG0680034985.01 | 0.813146 | 2.966478e-51 | 1.921795e-48 |
| MsG0280008383.01 | MsG0680035580.01 | 0.817470 | 3.242469e-52 | 2.363595e-49 |
| MsG0280008383.01 | MsG0680035707.01 | 0.808208 | 3.466625e-50 | 1.970295e-47 |
| MsG0280008383.01 | MsG0780036517.01 | 0.801002 | 1.105845e-48 | 5.223690e-46 |
| MsG0280008383.01 | MsG0780036593.01 | 0.847894 | 8.660573e-60 | 1.561889e-56 |
| MsG0280008383.01 | MsG0780036595.01 | 0.819404 | 1.182128e-52 | 9.088189e-50 |
| MsG0280008383.01 | MsG0780036596.01 | 0.829864 | 4.055197e-55 | 4.202978e-52 |
| MsG0280008383.01 | MsG0780036602.01 | 0.821737 | 3.443958e-53 | 2.825473e-50 |
| MsG0280008383.01 | MsG0780036757.01 | 0.806433 | 8.244485e-50 | 4.474283e-47 |
| MsG0280008383.01 | MsG0780037395.01 | 0.830528 | 2.790930e-55 | 2.948096e-52 |
| MsG0280008383.01 | MsG0780037808.01 | 0.813126 | 2.996246e-51 | 1.940017e-48 |
| MsG0280008383.01 | MsG0780038377.01 | 0.820197 | 7.790132e-53 | 6.123203e-50 |
| MsG0280008383.01 | MsG0780038744.01 | 0.801364 | 9.322672e-49 | 4.444021e-46 |
| MsG0280008383.01 | MsG0780038907.01 | 0.819352 | 1.215249e-52 | 9.328362e-50 |
| MsG0280008383.01 | MsG0780039139.01 | 0.811688 | 6.176700e-51 | 3.848546e-48 |
| MsG0280008383.01 | MsG0780039438.01 | 0.803275 | 3.768526e-49 | 1.885812e-46 |
| MsG0280008383.01 | MsG0780039867.01 | -0.812443 | 4.229511e-51 | 2.689730e-48 |
| MsG0280008383.01 | MsG0780040888.01 | 0.801975 | 6.985166e-49 | 3.381396e-46 |
| MsG0280008383.01 | MsG0780041074.01 | 0.823791 | 1.145419e-53 | 9.962024e-51 |
| MsG0280008383.01 | MsG0780041262.01 | 0.814601 | 1.417528e-51 | 9.553659e-49 |
| MsG0280008383.01 | MsG0780041391.01 | 0.808720 | 2.696433e-50 | 1.553034e-47 |
| MsG0280008383.01 | MsG0780041440.01 | 0.806827 | 6.808029e-50 | 3.732335e-47 |
| MsG0280008383.01 | MsG0780041468.01 | 0.832269 | 1.040861e-55 | 1.157403e-52 |
| MsG0280008383.01 | MsG0880041854.01 | 0.806766 | 7.013894e-50 | 3.839053e-47 |
| MsG0280008383.01 | MsG0880041861.01 | 0.835648 | 1.484653e-56 | 1.826914e-53 |
| MsG0280008383.01 | MsG0880041862.01 | 0.813409 | 2.597679e-51 | 1.695100e-48 |
| MsG0280008383.01 | MsG0880041863.01 | 0.806174 | 9.350737e-50 | 5.041412e-47 |
| MsG0280008383.01 | MsG0880041864.01 | 0.828372 | 9.327516e-55 | 9.253489e-52 |
| MsG0280008383.01 | MsG0880042276.01 | 0.843683 | 1.204811e-58 | 1.899732e-55 |
| MsG0280008383.01 | MsG0880042434.01 | 0.804490 | 2.107093e-49 | 1.087835e-46 |
| MsG0280008383.01 | MsG0880042656.01 | 0.813673 | 2.271903e-51 | 1.493161e-48 |
| MsG0280008383.01 | MsG0880043157.01 | 0.834385 | 3.090501e-56 | 3.661753e-53 |
| MsG0280008383.01 | MsG0880044043.01 | 0.825684 | 4.097781e-54 | 3.761493e-51 |
| MsG0280008383.01 | MsG0880044209.01 | 0.848994 | 4.296441e-60 | 8.029024e-57 |
| MsG0280008383.01 | MsG0880044487.01 | 0.815931 | 7.178541e-52 | 5.016395e-49 |
| MsG0280008383.01 | MsG0880044611.01 | -0.803035 | 4.225139e-49 | 2.101297e-46 |
| MsG0280008383.01 | MsG0880044902.01 | 0.810520 | 1.106555e-50 | 6.684651e-48 |
| MsG0280008383.01 | MsG0880045668.01 | 0.830518 | 2.807540e-55 | 2.964776e-52 |
| MsG0280008383.01 | MsG0880045722.01 | 0.824382 | 8.322210e-54 | 7.359290e-51 |
| MsG0280008383.01 | MsG0880045765.01 | 0.817686 | 2.899238e-52 | 2.125785e-49 |
| MsG0280008383.01 | MsG0880045992.01 | 0.810601 | 1.063133e-50 | 6.436285e-48 |
| MsG0280008383.01 | MsG0880046200.01 | 0.802526 | 5.380326e-49 | 2.641388e-46 |
| MsG0280008383.01 | MsG0880046457.01 | 0.818053 | 2.395844e-52 | 1.774384e-49 |
| MsG0280008383.01 | MsG0880047055.01 | -0.842661 | 2.256582e-58 | 3.445079e-55 |
| MsG0280008383.01 | MsG0880047394.01 | 0.828928 | 6.845637e-55 | 6.901426e-52 |
| MsG0280008383.01 | MsG0880047538.01 | 0.813152 | 2.958277e-51 | 1.916767e-48 |
| MsG0280008383.01 | MsG0880047754.01 | 0.805374 | 1.376841e-49 | 7.272249e-47 |
| MsG0280008383.01 | MsG0880047770.01 | 0.809040 | 2.303334e-50 | 1.338018e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008383.01.T01 | MTR_2g041310 | 100.000 | 114 | 0 | 0 | 21 | 134 | 1 | 114 | 5.45e-80 | 231 |
| MsG0280008383.01.T01 | MTR_4g125590 | 82.456 | 114 | 19 | 1 | 21 | 133 | 1 | 114 | 7.90e-64 | 190 |
| MsG0280008383.01.T01 | MTR_8g020200 | 70.175 | 114 | 34 | 0 | 21 | 134 | 1 | 114 | 1.24e-53 | 164 |
| MsG0280008383.01.T01 | MTR_3g070490 | 47.500 | 80 | 42 | 0 | 26 | 105 | 36 | 115 | 2.28e-22 | 85.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008383.01.T01 | AT1G17455 | 70.536 | 112 | 33 | 0 | 21 | 132 | 1 | 112 | 3.56e-51 | 158 |
| MsG0280008383.01.T01 | AT1G17455 | 70.536 | 112 | 33 | 0 | 21 | 132 | 1 | 112 | 3.56e-51 | 158 |
| MsG0280008383.01.T01 | AT1G72630 | 66.379 | 116 | 38 | 1 | 19 | 133 | 3 | 118 | 5.80e-49 | 153 |
| MsG0280008383.01.T01 | AT2G06255 | 68.696 | 115 | 29 | 3 | 21 | 134 | 1 | 109 | 3.19e-48 | 150 |
| MsG0280008383.01.T01 | AT2G29950 | 51.351 | 74 | 36 | 0 | 36 | 109 | 34 | 107 | 2.82e-20 | 80.5 |
| MsG0280008383.01.T01 | AT2G40080 | 48.571 | 70 | 36 | 0 | 36 | 105 | 22 | 91 | 5.48e-20 | 79.3 |
Find 35 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATTGTAACCTTGTAATTT+TGG | 0.117176 | 2:+30394488 | MsG0280008383.01.T01:three_prime_UTR |
| GCTGAGATTATCAGGAATTT+TGG | 0.137257 | 2:-30394084 | None:intergenic |
| CACCCTTCTGTTATTCAATA+TGG | 0.183221 | 2:+30394551 | MsG0280008383.01.T01:three_prime_UTR |
| TATATATTTAAAACATAATA+AGG | 0.206843 | 2:-30394431 | None:intergenic |
| TGTACAAGTGCAGAACATTT+TGG | 0.228918 | 2:+30394016 | MsG0280008383.01.T01:CDS |
| AAACTCTCTTCTTGTATTAC+AGG | 0.269823 | 2:+30394384 | MsG0280008383.01.T01:three_prime_UTR |
| ACATTTCTGCTGAGATTATC+AGG | 0.272078 | 2:-30394092 | None:intergenic |
| CAAATTGATACTAAGATATT+AGG | 0.308401 | 2:+30393975 | MsG0280008383.01.T01:CDS |
| AAGGTTACAATGAGAGGAAT+AGG | 0.337347 | 2:-30394478 | None:intergenic |
| TTGAGGTGTAGAACATGTTT+AGG | 0.366549 | 2:+30394343 | MsG0280008383.01.T01:three_prime_UTR |
| AGGTTACAATGAGAGGAATA+GGG | 0.383902 | 2:-30394477 | None:intergenic |
| GATGATACATTCTCTGGTAT+TGG | 0.392337 | 2:+30393942 | MsG0280008383.01.T01:CDS |
| ACATTCTCTGGTATTGGAAA+TGG | 0.427951 | 2:+30393948 | MsG0280008383.01.T01:CDS |
| TGACTCTAAAATTACAAGAA+TGG | 0.471081 | 2:+30393917 | MsG0280008383.01.T01:CDS |
| ATGGAGGATGATACATTCTC+TGG | 0.503053 | 2:+30393936 | MsG0280008383.01.T01:CDS |
| TCCATGGATGTTACTTCAGA+AGG | 0.520708 | 2:+30394194 | MsG0280008383.01.T01:CDS |
| TCCTTCTGAAGTAACATCCA+TGG | 0.529648 | 2:-30394195 | None:intergenic |
| TTTCCATATTGAATAACAGA+AGG | 0.535930 | 2:-30394554 | None:intergenic |
| TTAACTTCACAATCTTGTTG+AGG | 0.544380 | 2:+30394326 | MsG0280008383.01.T01:three_prime_UTR |
| GATAATCTCAGCAGAAATGT+TGG | 0.548630 | 2:+30394095 | MsG0280008383.01.T01:CDS |
| AACAAACCCTCTTTCTATGT+AGG | 0.557195 | 2:-30394272 | None:intergenic |
| TTATTAGATCCAAAATTACA+AGG | 0.559147 | 2:-30394497 | None:intergenic |
| ACACAGGCCTACATAGAAAG+AGG | 0.565404 | 2:+30394265 | MsG0280008383.01.T01:exon |
| TTCCATATTGAATAACAGAA+GGG | 0.568032 | 2:-30394553 | None:intergenic |
| AATTACAAGGTTACAATGAG+AGG | 0.582029 | 2:-30394484 | None:intergenic |
| AGTGGTGCTGTGAAATCAGA+TGG | 0.588608 | 2:+30394224 | MsG0280008383.01.T01:CDS |
| TACAATGAGAGGAATAGGGG+TGG | 0.602095 | 2:-30394473 | None:intergenic |
| CAACAACAATATTAGAAGAG+TGG | 0.606391 | 2:+30394133 | MsG0280008383.01.T01:CDS |
| CTCTAAAATTACAAGAATGG+AGG | 0.614344 | 2:+30393920 | MsG0280008383.01.T01:CDS |
| GTGAAATCAGATGGAAAAGG+TGG | 0.635941 | 2:+30394233 | MsG0280008383.01.T01:CDS |
| GGTTACAATGAGAGGAATAG+GGG | 0.635946 | 2:-30394476 | None:intergenic |
| CACAGGCCTACATAGAAAGA+GGG | 0.657963 | 2:+30394266 | MsG0280008383.01.T01:exon |
| ACTTCAGAAGGAGATTCAAG+TGG | 0.659737 | 2:+30394206 | MsG0280008383.01.T01:CDS |
| GCTGTGAAATCAGATGGAAA+AGG | 0.691564 | 2:+30394230 | MsG0280008383.01.T01:CDS |
| AAGGTGGACACAAAAGACAC+AGG | 0.696481 | 2:+30394249 | MsG0280008383.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAATTAATCATGTTTC+AGG | + | Chr2:30394517-30394536 | MsG0280008383.01.T01:three_prime_UTR | 15.0% |
| !!! | TTGTTTTTCTTAATTCTTTT+TGG | + | Chr2:30394290-30394309 | MsG0280008383.01.T01:three_prime_UTR | 15.0% |
| !! | CAAATTGATACTAAGATATT+AGG | + | Chr2:30393975-30393994 | MsG0280008383.01.T01:CDS | 20.0% |
| !! | TTATTAGATCCAAAATTACA+AGG | - | Chr2:30394500-30394519 | None:intergenic | 20.0% |
| ! | TTCCATATTGAATAACAGAA+GGG | - | Chr2:30394556-30394575 | None:intergenic | 25.0% |
| ! | TTGTTATATGTTATGATGCA+GGG | + | Chr2:30393863-30393882 | MsG0280008383.01.T01:intron | 25.0% |
| ! | TTTCCATATTGAATAACAGA+AGG | - | Chr2:30394557-30394576 | None:intergenic | 25.0% |
| !! | AAGTTCTTTTACTAAATCCA+TGG | + | Chr2:30394178-30394197 | MsG0280008383.01.T01:CDS | 25.0% |
| !! | GAATTTTGGATTCATGATTT+TGG | - | Chr2:30394073-30394092 | None:intergenic | 25.0% |
| !! | TCATTGTAACCTTGTAATTT+TGG | + | Chr2:30394488-30394507 | MsG0280008383.01.T01:three_prime_UTR | 25.0% |
| !! | TTTGTTATATGTTATGATGC+AGG | + | Chr2:30393862-30393881 | MsG0280008383.01.T01:intron | 25.0% |
| !!! | TCTTAATTCTTTTTGGTTTG+TGG | + | Chr2:30394297-30394316 | MsG0280008383.01.T01:three_prime_UTR | 25.0% |
| !!! | TGACTCTAAAATTACAAGAA+TGG | + | Chr2:30393917-30393936 | MsG0280008383.01.T01:CDS | 25.0% |
| AAACTCTCTTCTTGTATTAC+AGG | + | Chr2:30394384-30394403 | MsG0280008383.01.T01:three_prime_UTR | 30.0% | |
| AATTACAAGGTTACAATGAG+AGG | - | Chr2:30394487-30394506 | None:intergenic | 30.0% | |
| CAACAACAATATTAGAAGAG+TGG | + | Chr2:30394133-30394152 | MsG0280008383.01.T01:CDS | 30.0% | |
| TGTTTGTGTAAGTGCTATAT+AGG | + | Chr2:30393795-30393814 | MsG0280008383.01.T01:intron | 30.0% | |
| TTAACTTCACAATCTTGTTG+AGG | + | Chr2:30394326-30394345 | MsG0280008383.01.T01:three_prime_UTR | 30.0% | |
| !! | CTCTAAAATTACAAGAATGG+AGG | + | Chr2:30393920-30393939 | MsG0280008383.01.T01:CDS | 30.0% |
| !!! | TTTTAGAGTCAGAACTCAAA+AGG | - | Chr2:30393908-30393927 | None:intergenic | 30.0% |
| AACAAACCCTCTTTCTATGT+AGG | - | Chr2:30394275-30394294 | None:intergenic | 35.0% | |
| AAGGTTACAATGAGAGGAAT+AGG | - | Chr2:30394481-30394500 | None:intergenic | 35.0% | |
| ACATTTCTGCTGAGATTATC+AGG | - | Chr2:30394095-30394114 | None:intergenic | 35.0% | |
| AGGTTACAATGAGAGGAATA+GGG | - | Chr2:30394480-30394499 | None:intergenic | 35.0% | |
| CACCCTTCTGTTATTCAATA+TGG | + | Chr2:30394551-30394570 | MsG0280008383.01.T01:three_prime_UTR | 35.0% | |
| GATAATCTCAGCAGAAATGT+TGG | + | Chr2:30394095-30394114 | MsG0280008383.01.T01:CDS | 35.0% | |
| GATGATACATTCTCTGGTAT+TGG | + | Chr2:30393942-30393961 | MsG0280008383.01.T01:CDS | 35.0% | |
| TATATGTTATGATGCAGGGT+TGG | + | Chr2:30393867-30393886 | MsG0280008383.01.T01:intron | 35.0% | |
| TTGAGGTGTAGAACATGTTT+AGG | + | Chr2:30394343-30394362 | MsG0280008383.01.T01:three_prime_UTR | 35.0% | |
| ! | ACATTCTCTGGTATTGGAAA+TGG | + | Chr2:30393948-30393967 | MsG0280008383.01.T01:CDS | 35.0% |
| ! | AGAACATTTTGGATCAGAAC+AGG | + | Chr2:30394027-30394046 | MsG0280008383.01.T01:CDS | 35.0% |
| ! | GCTGAGATTATCAGGAATTT+TGG | - | Chr2:30394087-30394106 | None:intergenic | 35.0% |
| ! | TGTACAAGTGCAGAACATTT+TGG | + | Chr2:30394016-30394035 | MsG0280008383.01.T01:CDS | 35.0% |
| ACTTCAGAAGGAGATTCAAG+TGG | + | Chr2:30394206-30394225 | MsG0280008383.01.T01:CDS | 40.0% | |
| ATGGAGGATGATACATTCTC+TGG | + | Chr2:30393936-30393955 | MsG0280008383.01.T01:CDS | 40.0% | |
| GCTGTGAAATCAGATGGAAA+AGG | + | Chr2:30394230-30394249 | MsG0280008383.01.T01:CDS | 40.0% | |
| GGTTACAATGAGAGGAATAG+GGG | - | Chr2:30394479-30394498 | None:intergenic | 40.0% | |
| GTGAAATCAGATGGAAAAGG+TGG | + | Chr2:30394233-30394252 | MsG0280008383.01.T01:CDS | 40.0% | |
| ! | TCCATGGATGTTACTTCAGA+AGG | + | Chr2:30394194-30394213 | MsG0280008383.01.T01:CDS | 40.0% |
| !! | TCCTTCTGAAGTAACATCCA+TGG | - | Chr2:30394198-30394217 | None:intergenic | 40.0% |
| AAGGTGGACACAAAAGACAC+AGG | + | Chr2:30394249-30394268 | MsG0280008383.01.T01:CDS | 45.0% | |
| ACACAGGCCTACATAGAAAG+AGG | + | Chr2:30394265-30394284 | MsG0280008383.01.T01:exon | 45.0% | |
| CACAGGCCTACATAGAAAGA+GGG | + | Chr2:30394266-30394285 | MsG0280008383.01.T01:exon | 45.0% | |
| TACAATGAGAGGAATAGGGG+TGG | - | Chr2:30394476-30394495 | None:intergenic | 45.0% | |
| !! | AGTGGTGCTGTGAAATCAGA+TGG | + | Chr2:30394224-30394243 | MsG0280008383.01.T01:CDS | 45.0% |
| !!! | TATATATTTAAAACATAATA+AGG | - | Chr2:30394434-30394453 | None:intergenic | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 30393794 | 30394599 | 30393794 | ID=MsG0280008383.01;Name=MsG0280008383.01 |
| Chr2 | mRNA | 30393794 | 30394599 | 30393794 | ID=MsG0280008383.01.T01;Parent=MsG0280008383.01;Name=MsG0280008383.01.T01;_AED=0.41;_eAED=0.41;_QI=0|0|0.5|1|0|0.5|2|319|134 |
| Chr2 | exon | 30393794 | 30393801 | 30393794 | ID=MsG0280008383.01.T01:exon:5770;Parent=MsG0280008383.01.T01 |
| Chr2 | exon | 30393884 | 30394599 | 30393884 | ID=MsG0280008383.01.T01:exon:5771;Parent=MsG0280008383.01.T01 |
| Chr2 | CDS | 30393794 | 30393801 | 30393794 | ID=MsG0280008383.01.T01:cds;Parent=MsG0280008383.01.T01 |
| Chr2 | CDS | 30393884 | 30394280 | 30393884 | ID=MsG0280008383.01.T01:cds;Parent=MsG0280008383.01.T01 |
| Chr2 | three_prime_UTR | 30394281 | 30394599 | 30394281 | ID=MsG0280008383.01.T01:three_prime_utr;Parent=MsG0280008383.01.T01 |
| Gene Sequence |
| Protein sequence |
