AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780035995.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780035995.01.T02 MTR_7g006630 80.556 36 7 0 2 37 138 173 9.37e-16 65.9
MsG0780035995.01.T02 MTR_7g006690 77.778 36 8 0 2 37 143 178 2.83e-15 64.7
MsG0780035995.01.T02 MTR_6g033040 76.471 34 8 0 2 35 142 175 1.56e-12 57.8
MsG0780035995.01.T02 MTR_7g084970 72.727 33 9 0 1 33 141 173 1.15e-11 55.5
MsG0780035995.01.T01 MTR_7g006690 86.585 82 11 0 1 82 70 151 1.46e-50 157
MsG0780035995.01.T01 MTR_7g006630 84.146 82 13 0 1 82 65 146 5.77e-49 153
MsG0780035995.01.T01 MTR_6g033040 62.195 82 31 0 1 82 69 150 3.10e-35 119
MsG0780035995.01.T01 MTR_7g084970 57.647 85 36 0 1 85 69 153 4.85e-33 113
MsG0780035995.01.T01 MTR_0020s0120 59.259 81 33 0 1 81 68 148 1.97e-32 111
MsG0780035995.01.T01 MTR_7g085020 59.756 82 33 0 1 82 70 151 2.23e-32 111
MsG0780035995.01.T01 MTR_1g060190 58.228 79 31 1 1 77 69 147 1.15e-29 104
MsG0780035995.01.T01 MTR_7g085040 52.941 85 40 0 1 85 66 150 7.25e-29 102
MsG0780035995.01.T01 MTR_7g104460 56.250 80 34 1 1 79 69 148 1.29e-27 99.8
MsG0780035995.01.T01 MTR_0020s0120 55.556 81 29 1 1 81 68 141 3.59e-27 98.2
MsG0780035995.01.T01 MTR_2g086270 48.780 82 41 1 1 81 68 149 7.78e-25 92.4
MsG0780035995.01.T01 MTR_8g106840 47.436 78 40 1 1 77 68 145 4.43e-18 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780035995.01.T02 AT1G65480 73.529 34 9 0 2 35 186 219 7.96e-14 61.6
MsG0780035995.01.T02 AT1G65480 73.529 34 9 0 2 35 142 175 9.70e-14 60.8
MsG0780035995.01.T02 AT4G20370 73.529 34 9 0 1 34 141 174 2.50e-13 59.7
MsG0780035995.01.T01 AT1G65480 65.432 81 28 0 1 81 69 149 1.42e-35 119
MsG0780035995.01.T01 AT1G65480 65.432 81 28 0 1 81 113 193 4.02e-35 120
MsG0780035995.01.T01 AT4G20370 58.025 81 34 0 1 81 69 149 2.91e-31 108
MsG0780035995.01.T01 AT5G62040 54.217 83 37 1 1 82 69 151 5.19e-28 100
MsG0780035995.01.T01 AT5G03840 52.041 98 40 4 1 96 72 164 1.43e-26 97.1
MsG0780035995.01.T01 AT2G27550 50.000 80 39 1 1 79 70 149 7.05e-25 92.8
MsG0780035995.01.T01 AT1G18100 51.899 79 36 1 1 77 67 145 3.03e-22 85.9

Find 30 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGCAGAAGTTTACAATCT+TGG 0.279344 7:-1070248 MsG0780035995.01.T01:CDS
TGAATCCCTCTTGTTGGTCT+TGG 0.316500 7:+1070355 None:intergenic
CCCTGTAGTCGCTGGAATAT+TGG 0.340527 7:+1070725 None:intergenic
TCAGTAGGGCTTGGTGCATC+AGG 0.356176 7:+1072298 None:intergenic
TACAGGTATTCCCTCATATT+AGG 0.366238 7:+1072274 None:intergenic
TTGTGGTTATAATTATTAGA+TGG 0.404057 7:+1070160 None:intergenic
TGTTGTTTAAGCAACCTTGT+AGG 0.415361 7:-1070318 MsG0780035995.01.T01:CDS
GCCCTACTGATCCTAATATG+AGG 0.445100 7:-1072285 MsG0780035995.01.T01:CDS
TGTCAACGAGAAAGTGGTTC+CGG 0.448740 7:-1070197 MsG0780035995.01.T01:CDS
ACCAATATTCCAGCGACTAC+AGG 0.450659 7:-1070726 MsG0780035995.01.T01:CDS
ATATGAGGGAATACCTGTAT+TGG 0.453295 7:-1072270 MsG0780035995.01.T01:intron
ACACGATGAATCCCTCTTGT+TGG 0.458184 7:+1070349 None:intergenic
TTCAATTGTCAACGAGAAAG+TGG 0.476637 7:-1070203 MsG0780035995.01.T01:CDS
TTGAAATAGAGAGCAGCAAC+AGG 0.483630 7:+1070221 None:intergenic
AAATTCAAACTTACCAATAC+AGG 0.502537 7:+1072257 None:intergenic
GCGACTACAGGGACAACATT+CGG 0.503478 7:-1070714 MsG0780035995.01.T01:intron
AATGTTGTCCCTGTAGTCGC+TGG 0.506880 7:+1070717 None:intergenic
ATATTAGGATCAGTAGGGCT+TGG 0.509781 7:+1072289 None:intergenic
GCTAATATAGTGTGCCTACA+AGG 0.535198 7:+1070304 None:intergenic
GATGGTTAGCCTCCTTCCAC+CGG 0.542124 7:+1070178 None:intergenic
GAGAAAGTGGTTCCGGTGGA+AGG 0.542228 7:-1070190 MsG0780035995.01.T01:CDS
CAGTAGGGCTTGGTGCATCA+GGG 0.543914 7:+1072299 None:intergenic
CCCTCATATTAGGATCAGTA+GGG 0.548872 7:+1072284 None:intergenic
CCCTACTGATCCTAATATGA+GGG 0.564661 7:-1072284 MsG0780035995.01.T01:CDS
GAAAATCCAAGACCAACAAG+AGG 0.566107 7:-1070361 MsG0780035995.01.T01:CDS
TCCCTCATATTAGGATCAGT+AGG 0.580960 7:+1072283 None:intergenic
CCAATATTCCAGCGACTACA+GGG 0.601358 7:-1070725 MsG0780035995.01.T01:CDS
AAAATCCAAGACCAACAAGA+GGG 0.616950 7:-1070360 MsG0780035995.01.T01:CDS
CAACGAGAAAGTGGTTCCGG+TGG 0.628002 7:-1070194 MsG0780035995.01.T01:CDS
AAAGTGGTTCCGGTGGAAGG+AGG 0.632372 7:-1070187 MsG0780035995.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCCAAAAATATTATAAAT+TGG + Chr7:1070800-1070819 None:intergenic 10.0%
!! TGTATAAATATTCAAAAAAA+TGG + Chr7:1070840-1070859 None:intergenic 10.0%
!! TTATTTATTAAAGATCTATT+TGG + Chr7:1070636-1070655 None:intergenic 10.0%
!!! ATACATTTTTATATATAACA+AGG - Chr7:1071886-1071905 MsG0780035995.01.T01:intron 10.0%
!!! ATTTTTTTAATAATTTAGCA+TGG - Chr7:1070996-1071015 MsG0780035995.01.T01:intron 10.0%
!!! TAATATTTTTGGAATTTATA+AGG - Chr7:1070805-1070824 MsG0780035995.01.T01:intron 10.0%
!!! TACATTTTTATATATAACAA+GGG - Chr7:1071887-1071906 MsG0780035995.01.T01:intron 10.0%
!!! TCATATTTTTTATTTTGTTT+CGG - Chr7:1070291-1070310 MsG0780035995.01.T01:CDS 10.0%
!! CAAAATAAAAAATATGATTG+TGG + Chr7:1070288-1070307 None:intergenic 15.0%
!! TGATAAATATTATTATGTCT+TGG - Chr7:1070323-1070342 MsG0780035995.01.T01:CDS 15.0%
!!! AAAAAAAAAGTGTTGTATTT+AGG - Chr7:1071458-1071477 MsG0780035995.01.T01:intron 15.0%
!!! CAACCAATTTATAATATTTT+TGG - Chr7:1070794-1070813 MsG0780035995.01.T01:intron 15.0%
!!! TATATTTTTCATGAATTCAA+AGG + Chr7:1071150-1071169 None:intergenic 15.0%
!! ATCAAAATAAAGATAAACAG+TGG + Chr7:1071301-1071320 None:intergenic 20.0%
!! GAAAAATATAACTTCAATTG+TGG - Chr7:1071160-1071179 MsG0780035995.01.T01:intron 20.0%
!! TAGTGTAAGAACAAAAATTT+GGG + Chr7:1070888-1070907 None:intergenic 20.0%
!! TATTCAAAAAAATGGTAAGT+TGG + Chr7:1070832-1070851 None:intergenic 20.0%
!! TATTTAGGTCAGAAAATAAT+TGG - Chr7:1071473-1071492 MsG0780035995.01.T01:intron 20.0%
!! TCAAAATAAAGATAAACAGT+GGG + Chr7:1071300-1071319 None:intergenic 20.0%
!! TTAGTGTAAGAACAAAAATT+TGG + Chr7:1070889-1070908 None:intergenic 20.0%
!!! GTTTATCTTTATTTTGATGA+TGG - Chr7:1071303-1071322 MsG0780035995.01.T01:intron 20.0%
! AAAAATAAGCCAAACAAAAG+AGG + Chr7:1070445-1070464 None:intergenic 25.0%
! AAATTCAAACTTACCAATAC+AGG + Chr7:1070228-1070247 None:intergenic 25.0%
! AATACACAACTTATGATTTG+CGG - Chr7:1070961-1070980 MsG0780035995.01.T01:intron 25.0%
! ATAGTTCATTCTTTGCAATT+TGG - Chr7:1071250-1071269 MsG0780035995.01.T01:intron 25.0%
! ATGCATTTGAACAATGTTTA+TGG - Chr7:1071353-1071372 MsG0780035995.01.T01:intron 25.0%
!! TTTTTATATATAACAAGGGC+CGG - Chr7:1071891-1071910 MsG0780035995.01.T01:intron 25.0%
!! TTTTTGATAAGTCAATTCAC+TGG + Chr7:1070940-1070959 None:intergenic 25.0%
!!! CACGTTGTTTTATTAATTGT+AGG - Chr7:1072079-1072098 MsG0780035995.01.T01:intron 25.0%
!!! TAATTTTAAGAGTGCGTTTT+AGG - Chr7:1070419-1070438 MsG0780035995.01.T01:intron 25.0%
ACCATTTCCCTCTTTATTAT+TGG - Chr7:1071714-1071733 MsG0780035995.01.T01:intron 30.0%
ATAGCGATCCAATAATAAAG+AGG + Chr7:1071725-1071744 None:intergenic 30.0%
CTATAGGACTTAAATTTGGT+TGG + Chr7:1070913-1070932 None:intergenic 30.0%
TAGCGATCCAATAATAAAGA+GGG + Chr7:1071724-1071743 None:intergenic 30.0%
TCCAATAATAAAGAGGGAAA+TGG + Chr7:1071718-1071737 None:intergenic 30.0%
TTATTATTGGATCGCTATAC+AGG - Chr7:1071727-1071746 MsG0780035995.01.T01:intron 30.0%
TTTGCAGAAGTTTACAATCT+TGG - Chr7:1072234-1072253 MsG0780035995.01.T01:intron 30.0%
! ATAAAATTTTGTCTCGATCG+AGG + Chr7:1072205-1072224 None:intergenic 30.0%
! ATACACAACTTATGATTTGC+GGG - Chr7:1070962-1070981 MsG0780035995.01.T01:intron 30.0%
! TGGCTAGAATTCACATTTTA+AGG + Chr7:1071946-1071965 None:intergenic 30.0%
!!! TTTTTTGTCAAGTAGTCTAG+TGG + Chr7:1071966-1071985 None:intergenic 30.0%
AAAATCCAAGACCAACAAGA+GGG - Chr7:1072122-1072141 MsG0780035995.01.T01:intron 35.0%
ATATGAGGGAATACCTGTAT+TGG - Chr7:1070212-1070231 MsG0780035995.01.T01:CDS 35.0%
CTTTATTCTATTCGCTCACA+AGG + Chr7:1071400-1071419 None:intergenic 35.0%
GAGGGAAATGGTAATTAGAA+TGG + Chr7:1071706-1071725 None:intergenic 35.0%
GGATAAACAATAGAACCAAG+TGG - Chr7:1071181-1071200 MsG0780035995.01.T01:intron 35.0%
GTGATGTGATAGACAAAACA+AGG - Chr7:1071572-1071591 MsG0780035995.01.T01:intron 35.0%
TACAGGTATTCCCTCATATT+AGG + Chr7:1070211-1070230 None:intergenic 35.0%
TTCAATTGTCAACGAGAAAG+TGG - Chr7:1072279-1072298 MsG0780035995.01.T01:CDS 35.0%
TTGATAAGTCAATTCACTGG+CGG + Chr7:1070937-1070956 None:intergenic 35.0%
! TCACTCATGTTTGTTGAACT+TGG + Chr7:1071102-1071121 None:intergenic 35.0%
! TGTTGTTTAAGCAACCTTGT+AGG - Chr7:1072164-1072183 MsG0780035995.01.T01:intron 35.0%
!! CTTGGTATTGTTTTGTTCAG+TGG - Chr7:1070341-1070360 MsG0780035995.01.T01:CDS 35.0%
!!! CACATTTTAAGGTGAGTAAG+TGG + Chr7:1071935-1071954 None:intergenic 35.0%
ATATTAGGATCAGTAGGGCT+TGG + Chr7:1070196-1070215 None:intergenic 40.0%
CCCTACTGATCCTAATATGA+GGG - Chr7:1070198-1070217 MsG0780035995.01.T01:CDS 40.0%
CCCTCATATTAGGATCAGTA+GGG + Chr7:1070201-1070220 None:intergenic 40.0%
CGGGCTATAGGACTTAAATT+TGG + Chr7:1070917-1070936 None:intergenic 40.0%
GAAAATCCAAGACCAACAAG+AGG - Chr7:1072121-1072140 MsG0780035995.01.T01:intron 40.0%
GCTAATATAGTGTGCCTACA+AGG + Chr7:1072181-1072200 None:intergenic 40.0%
GTCTCTGAGTATAGATCAGT+TGG - Chr7:1071855-1071874 MsG0780035995.01.T01:intron 40.0%
TATTGGATCGCTATACAGGA+TGG - Chr7:1071731-1071750 MsG0780035995.01.T01:intron 40.0%
TCCCTCATATTAGGATCAGT+AGG + Chr7:1070202-1070221 None:intergenic 40.0%
TGATAAGTCAATTCACTGGC+GGG + Chr7:1070936-1070955 None:intergenic 40.0%
TTGAAATAGAGAGCAGCAAC+AGG + Chr7:1072264-1072283 None:intergenic 40.0%
!! CGTTTTAGGCCTCTTTTGTT+TGG - Chr7:1070433-1070452 MsG0780035995.01.T01:intron 40.0%
AAGGTGAGTAAGTGGAGTGT+CGG + Chr7:1071927-1071946 None:intergenic 45.0%
ACACGATGAATCCCTCTTGT+TGG + Chr7:1072136-1072155 None:intergenic 45.0%
ACCAATATTCCAGCGACTAC+AGG - Chr7:1071756-1071775 MsG0780035995.01.T01:intron 45.0%
ACTTGGTCACATCTTGTGCA+TGG + Chr7:1071085-1071104 None:intergenic 45.0%
CACAGACACAAGAAACCACT+TGG + Chr7:1071199-1071218 None:intergenic 45.0%
CCAATATTCCAGCGACTACA+GGG - Chr7:1071757-1071776 MsG0780035995.01.T01:intron 45.0%
GCCCTACTGATCCTAATATG+AGG - Chr7:1070197-1070216 MsG0780035995.01.T01:CDS 45.0%
TGTCAACGAGAAAGTGGTTC+CGG - Chr7:1072285-1072304 MsG0780035995.01.T01:CDS 45.0%
! ATTCGCTCACAAGGTGTCTA+CGG + Chr7:1071391-1071410 None:intergenic 45.0%
! TGAATCCCTCTTGTTGGTCT+TGG + Chr7:1072130-1072149 None:intergenic 45.0%
!! TTTCTATATAATTAAAAAAA+TGG - Chr7:1070711-1070730 MsG0780035995.01.T01:intron 5.0%
AATGTTGTCCCTGTAGTCGC+TGG + Chr7:1071768-1071787 None:intergenic 50.0%
CCCTGTAGTCGCTGGAATAT+TGG + Chr7:1071760-1071779 None:intergenic 50.0%
GCGACTACAGGGACAACATT+CGG - Chr7:1071768-1071787 MsG0780035995.01.T01:intron 50.0%
! AGGTGAGTAAGTGGAGTGTC+GGG + Chr7:1071926-1071945 None:intergenic 50.0%
! TTCGCTCACAAGGTGTCTAC+GGG + Chr7:1071390-1071409 None:intergenic 50.0%
!! TCAATTCACTGGCGGGCTAT+AGG + Chr7:1070929-1070948 None:intergenic 50.0%
AAAGTGGTTCCGGTGGAAGG+AGG - Chr7:1072295-1072314 MsG0780035995.01.T01:CDS 55.0%
CAACGAGAAAGTGGTTCCGG+TGG - Chr7:1072288-1072307 MsG0780035995.01.T01:CDS 55.0%
GAGAAAGTGGTTCCGGTGGA+AGG - Chr7:1072292-1072311 MsG0780035995.01.T01:CDS 55.0%
! CAAGGTGTCTACGGGATGGA+TGG + Chr7:1071382-1071401 None:intergenic 55.0%
! CTCACAAGGTGTCTACGGGA+TGG + Chr7:1071386-1071405 None:intergenic 55.0%
!! CAGTAGGGCTTGGTGCATCA+GGG + Chr7:1070186-1070205 None:intergenic 55.0%
!! GATGGTTAGCCTCCTTCCAC+CGG + Chr7:1072307-1072326 None:intergenic 55.0%
!! GGTGAGTAAGTGGAGTGTCG+GGG + Chr7:1071925-1071944 None:intergenic 55.0%
!! TCAGTAGGGCTTGGTGCATC+AGG + Chr7:1070187-1070206 None:intergenic 55.0%
!! GAGTGTCGGGGTTCGAACAC+CGG + Chr7:1071913-1071932 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 1070175 1072329 1070175 ID=MsG0780035995.01;Name=MsG0780035995.01
Chr7 mRNA 1070183 1072329 1070183 ID=MsG0780035995.01.T01;Parent=MsG0780035995.01;Name=MsG0780035995.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|0|0|3|0|106
Chr7 exon 1070183 1070403 1070183 ID=MsG0780035995.01.T01:exon:2233;Parent=MsG0780035995.01.T01
Chr7 exon 1070715 1070755 1070715 ID=MsG0780035995.01.T01:exon:2232;Parent=MsG0780035995.01.T01
Chr7 exon 1072271 1072329 1072271 ID=MsG0780035995.01.T01:exon:2231;Parent=MsG0780035995.01.T01
Chr7 CDS 1072271 1072329 1072271 ID=MsG0780035995.01.T01:cds;Parent=MsG0780035995.01.T01
Chr7 CDS 1070715 1070755 1070715 ID=MsG0780035995.01.T01:cds;Parent=MsG0780035995.01.T01
Chr7 CDS 1070183 1070403 1070183 ID=MsG0780035995.01.T01:cds;Parent=MsG0780035995.01.T01
Chr7 mRNA 1070175 1070288 1070175 ID=MsG0780035995.01.T02;Parent=MsG0780035995.01;Name=MsG0780035995.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|37
Chr7 exon 1070175 1070288 1070175 ID=MsG0780035995.01.T02:exon:2234;Parent=MsG0780035995.01.T02
Chr7 CDS 1070175 1070288 1070175 ID=MsG0780035995.01.T02:cds;Parent=MsG0780035995.01.T02
Chr7 mRNA 1070347 1070385 1070347 ID=MsG0780035995.01.T03;Parent=MsG0780035995.01;Name=MsG0780035995.01.T03;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|12
Chr7 exon 1070347 1070385 1070347 ID=MsG0780035995.01.T03:exon:2235;Parent=MsG0780035995.01.T03
Chr7 CDS 1070347 1070385 1070347 ID=MsG0780035995.01.T03:cds;Parent=MsG0780035995.01.T03
Gene Sequence

>MsG0780035995.01.T03

ATGAAAATCCAAGACCAACAAGAGGGATTCATCGTGTGA

>MsG0780035995.01.T02

AAATTTTATTACAAGAGATTTGCAGAAGTTTACAATCTTGGATTACCTGTTGCTGCTCTCTATTTCAATTGTCAACGAGAAAGTGGTTCCGGTGGAAGGAGGCTAACCATCTAA

>MsG0780035995.01.T01

ATGGTGAACCCTGATGCACCAAGCCCTACTGATCCTAATATGAGGGAATACCTGTATTGGATGGTTACCAATATTCCAGCGACTACAGGGACAACATTCGGACAAGAGATAGTGAGCTATGAAAATCCAAGACCAACAAGAGGGATTCATCGTGTGATATTTGTGTTGTTTAAGCAACCTTGTAGGCACACTATATTAGCTCCTCGATCGAGACAAAATTTTATTACAAGAGATTTGCAGAAGTTTACAATCTTGGATTACCTGTTGCTGCTCTCTATTTCAATTGTCAACGAGAAAGTGGTTCCGGTGGAAGGAGGCTAA

Protein sequence

>MsG0780035995.01.T03

MKIQDQQEGFIV*

>MsG0780035995.01.T02

KFYYKRFAEVYNLGLPVAALYFNCQRESGSGGRRLTI*

>MsG0780035995.01.T01

MVNPDAPSPTDPNMREYLYWMVTNIPATTGTTFGQEIVSYENPRPTRGIHRVIFVLFKQPCRHTILAPRSRQNFITRDLQKFTILDYLLLLSISIVNEKVVPVEGG*