AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031866.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031866.01.T01 MTR_6g034060 96.624 237 8 0 39 275 1 237 1.52e-169 468
MsG0680031866.01.T01 MTR_6g034055 77.872 235 33 3 39 273 1 216 2.93e-116 333
MsG0680031866.01.T01 MTR_6g034050 61.277 235 61 4 39 273 1 205 5.44e-84 251
MsG0680031866.01.T01 MTR_1g099440 63.983 236 65 9 39 273 1 217 3.94e-81 244
MsG0680031866.01.T01 MTR_5g039840 29.958 237 130 8 39 274 1 202 1.90e-13 68.2
MsG0680031866.01.T01 MTR_7g039720 29.752 242 122 8 39 273 1 201 1.77e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031866.01.T01 AT5G52900 40.400 250 118 12 36 273 3 233 1.47e-33 122

Find 44 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACTAGTGATGATGGTTTA+AGG 0.126697 6:+28488027 None:intergenic
GCTTCTTCTATCTTACTCTT+TGG 0.194146 6:+28488107 None:intergenic
CTTCTAGAAGATGGCATTCT+TGG 0.216433 6:+28487930 None:intergenic
TTTATTTGTTACATGTGATA+TGG 0.284843 6:+28487704 None:intergenic
AGCTTCTTCCTTCATTGAAA+TGG 0.311354 6:-28487955 MsG0680031866.01.T01:CDS
TTCAATGATTCAACAAATAA+TGG 0.323139 6:+28487798 None:intergenic
AAGAATATTAGAGGAAATTA+TGG 0.323873 6:-28487513 MsG0680031866.01.T01:CDS
AGTGAGAGGGGATTGTTGTA+AGG 0.366132 6:+28487866 None:intergenic
GAATATGCAACACTAATTCT+TGG 0.384978 6:+28487480 None:intergenic
ATTCAACAAATAATGGCATT+AGG 0.397396 6:+28487805 None:intergenic
TTTGATGAATCATATTCATA+TGG 0.399205 6:+28487768 None:intergenic
CAATCAGTGAAGAATATTAG+AGG 0.410239 6:-28487522 MsG0680031866.01.T01:CDS
TCACTAGTAGAAAGCCTTGA+TGG 0.413442 6:-28488014 MsG0680031866.01.T01:CDS
TTGCATATTCCGCGGATGAT+TGG 0.414207 6:-28487467 MsG0680031866.01.T01:CDS
AGAGGGGATTGTTGTAAGGT+TGG 0.430996 6:+28487870 None:intergenic
CAAGAAGCCCTTCATTGAAA+AGG 0.435742 6:-28487662 MsG0680031866.01.T01:CDS
ATGAATCTTTGAAGATAACT+TGG 0.435778 6:+28488130 None:intergenic
GCCTTTGTGTAGAAGATTGA+GGG 0.450787 6:-28487589 MsG0680031866.01.T01:CDS
AGGCTTTCTACTAGTGATGA+TGG 0.454755 6:+28488020 None:intergenic
TGGAAAGTGTGAGGGAAAGA+AGG 0.460669 6:+28488150 None:intergenic
TCTTGGATCCATTTCAATGA+AGG 0.466142 6:+28487947 None:intergenic
CATATTCCGCGGATGATTGG+CGG 0.479193 6:-28487464 MsG0680031866.01.T01:CDS
CTTCATTGCAAAAGATCCAT+TGG 0.491601 6:-28487399 MsG0680031866.01.T01:CDS
GGCCTTTGTGTAGAAGATTG+AGG 0.502821 6:-28487590 MsG0680031866.01.T01:CDS
GAGTAAGATAGAAGAAGCCA+TGG 0.511798 6:-28488102 MsG0680031866.01.T01:CDS
TAATGGCATTAGGTAACCAT+TGG 0.512546 6:+28487815 None:intergenic
ATCACATGTAACAAATAAAG+TGG 0.516247 6:-28487700 MsG0680031866.01.T01:CDS
TTCATCAGAAGAATCAAGAT+AGG 0.516867 6:+28487977 None:intergenic
GGAAAGTGTGAGGGAAAGAA+GGG 0.527768 6:+28488151 None:intergenic
ATGTGATATGGAAGAAGCTA+AGG 0.537039 6:+28487716 None:intergenic
GAGAGGATGAGATGTTTCCA+TGG 0.551109 6:+28488085 None:intergenic
AAGATAACTTGGAAAGTGTG+AGG 0.569745 6:+28488141 None:intergenic
TTCTAAAAGAAGAACCATCA+AGG 0.577711 6:+28488000 None:intergenic
AAAGGTGTAGAACATTATCA+AGG 0.580857 6:-28487644 MsG0680031866.01.T01:CDS
CACGCGCCGCCAATCATCCG+CGG 0.598671 6:+28487458 None:intergenic
AGATAACTTGGAAAGTGTGA+GGG 0.605881 6:+28488142 None:intergenic
CATCAACAAGAGTAGTGAGA+GGG 0.614657 6:+28487853 None:intergenic
GGGCTTCTTGAATCTTCTCG+AGG 0.622817 6:+28487675 None:intergenic
AATTAGTGTTGCATATTCCG+CGG 0.626302 6:-28487475 MsG0680031866.01.T01:CDS
AAATGAGTTGAAGATAGCAA+AGG 0.638354 6:+28488179 None:intergenic
GCATCAACAAGAGTAGTGAG+AGG 0.665674 6:+28487852 None:intergenic
CCCCTCAATCTTCTACACAA+AGG 0.666242 6:+28487588 None:intergenic
CCTTTGTGTAGAAGATTGAG+GGG 0.676588 6:-28487588 MsG0680031866.01.T01:CDS
ATCAACAAGAGTAGTGAGAG+GGG 0.692218 6:+28487854 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGTATTTGAAATTTTTA+AGG - Chr6:28487978-28487997 MsG0680031866.01.T01:CDS 10.0%
!! AAGAATATTAGAGGAAATTA+TGG - Chr6:28488076-28488095 MsG0680031866.01.T01:CDS 20.0%
!! TTCAATGATTCAACAAATAA+TGG + Chr6:28487794-28487813 None:intergenic 20.0%
!! TTTGATGAATCATATTCATA+TGG + Chr6:28487824-28487843 None:intergenic 20.0%
!!! ATAAAAAATCTTCTAGAAGA+TGG + Chr6:28487671-28487690 None:intergenic 20.0%
!!! TAGAAAGTTTTTCATATAGT+TGG - Chr6:28487531-28487550 MsG0680031866.01.T01:CDS 20.0%
!!! TTTATTTGTTACATGTGATA+TGG + Chr6:28487888-28487907 None:intergenic 20.0%
! ATCACATGTAACAAATAAAG+TGG - Chr6:28487889-28487908 MsG0680031866.01.T01:CDS 25.0%
! ATTCAACAAATAATGGCATT+AGG + Chr6:28487787-28487806 None:intergenic 25.0%
!! ATGAATCTTTGAAGATAACT+TGG + Chr6:28487462-28487481 None:intergenic 25.0%
CAATCAGTGAAGAATATTAG+AGG - Chr6:28488067-28488086 MsG0680031866.01.T01:CDS 30.0%
GAAAAACTTTCTATTGAGAG+AGG + Chr6:28487524-28487543 None:intergenic 30.0%
GAATATGCAACACTAATTCT+TGG + Chr6:28488112-28488131 None:intergenic 30.0%
TTCATCAGAAGAATCAAGAT+AGG + Chr6:28487615-28487634 None:intergenic 30.0%
! AAAGGTGTAGAACATTATCA+AGG - Chr6:28487945-28487964 MsG0680031866.01.T01:CDS 30.0%
! AAATGAGTTGAAGATAGCAA+AGG + Chr6:28487413-28487432 None:intergenic 30.0%
! TTCTAAAAGAAGAACCATCA+AGG + Chr6:28487592-28487611 None:intergenic 30.0%
! TTCTACACCTTTTCAATGAA+GGG + Chr6:28487937-28487956 None:intergenic 30.0%
AATTAGTGTTGCATATTCCG+CGG - Chr6:28488114-28488133 MsG0680031866.01.T01:CDS 35.0%
AGCTTCTTCCTTCATTGAAA+TGG - Chr6:28487634-28487653 MsG0680031866.01.T01:CDS 35.0%
ATGTGATATGGAAGAAGCTA+AGG + Chr6:28487876-28487895 None:intergenic 35.0%
CTTCATTGCAAAAGATCCAT+TGG - Chr6:28488190-28488209 MsG0680031866.01.T01:CDS 35.0%
GCTTCTTCTATCTTACTCTT+TGG + Chr6:28487485-28487504 None:intergenic 35.0%
TAATGGCATTAGGTAACCAT+TGG + Chr6:28487777-28487796 None:intergenic 35.0%
TCTTGGATCCATTTCAATGA+AGG + Chr6:28487645-28487664 None:intergenic 35.0%
! AAGATAACTTGGAAAGTGTG+AGG + Chr6:28487451-28487470 None:intergenic 35.0%
! AGATAACTTGGAAAGTGTGA+GGG + Chr6:28487450-28487469 None:intergenic 35.0%
! GTTCTACACCTTTTCAATGA+AGG + Chr6:28487938-28487957 None:intergenic 35.0%
!! CTACTAGTGATGATGGTTTA+AGG + Chr6:28487565-28487584 None:intergenic 35.0%
AGATTGAGGGGTCAAAAATC+AGG - Chr6:28488013-28488032 MsG0680031866.01.T01:CDS 40.0%
AGGCTTTCTACTAGTGATGA+TGG + Chr6:28487572-28487591 None:intergenic 40.0%
ATCAACAAGAGTAGTGAGAG+GGG + Chr6:28487738-28487757 None:intergenic 40.0%
CAAGAAGCCCTTCATTGAAA+AGG - Chr6:28487927-28487946 MsG0680031866.01.T01:CDS 40.0%
CATCAACAAGAGTAGTGAGA+GGG + Chr6:28487739-28487758 None:intergenic 40.0%
CCTTTGTGTAGAAGATTGAG+GGG - Chr6:28488001-28488020 MsG0680031866.01.T01:CDS 40.0%
GAGTAAGATAGAAGAAGCCA+TGG - Chr6:28487487-28487506 MsG0680031866.01.T01:CDS 40.0%
GCCTTTGTGTAGAAGATTGA+GGG - Chr6:28488000-28488019 MsG0680031866.01.T01:CDS 40.0%
TCACTAGTAGAAAGCCTTGA+TGG - Chr6:28487575-28487594 MsG0680031866.01.T01:CDS 40.0%
! CTTCTAGAAGATGGCATTCT+TGG + Chr6:28487662-28487681 None:intergenic 40.0%
! GCAGATCAACTCTTTTCCAA+TGG - Chr6:28487758-28487777 MsG0680031866.01.T01:CDS 40.0%
AGAGGGGATTGTTGTAAGGT+TGG + Chr6:28487722-28487741 None:intergenic 45.0%
AGTGAGAGGGGATTGTTGTA+AGG + Chr6:28487726-28487745 None:intergenic 45.0%
CCCCTCAATCTTCTACACAA+AGG + Chr6:28488004-28488023 None:intergenic 45.0%
GAGAGGATGAGATGTTTCCA+TGG + Chr6:28487507-28487526 None:intergenic 45.0%
GCATCAACAAGAGTAGTGAG+AGG + Chr6:28487740-28487759 None:intergenic 45.0%
GGCCTTTGTGTAGAAGATTG+AGG - Chr6:28487999-28488018 MsG0680031866.01.T01:CDS 45.0%
TTGCATATTCCGCGGATGAT+TGG - Chr6:28488122-28488141 MsG0680031866.01.T01:CDS 45.0%
! GGAAAGTGTGAGGGAAAGAA+GGG + Chr6:28487441-28487460 None:intergenic 45.0%
! TGGAAAGTGTGAGGGAAAGA+AGG + Chr6:28487442-28487461 None:intergenic 45.0%
CATATTCCGCGGATGATTGG+CGG - Chr6:28488125-28488144 MsG0680031866.01.T01:CDS 50.0%
GGGCTTCTTGAATCTTCTCG+AGG + Chr6:28487917-28487936 None:intergenic 50.0%
CACGCGCCGCCAATCATCCG+CGG + Chr6:28488134-28488153 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr6 gene 28487392 28488219 28487392 ID=MsG0680031866.01;Name=MsG0680031866.01
Chr6 mRNA 28487392 28488219 28487392 ID=MsG0680031866.01.T01;Parent=MsG0680031866.01;Name=MsG0680031866.01.T01;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|275
Chr6 exon 28487392 28488219 28487392 ID=MsG0680031866.01.T01:exon:14579;Parent=MsG0680031866.01.T01
Chr6 CDS 28487392 28488219 28487392 ID=MsG0680031866.01.T01:cds;Parent=MsG0680031866.01.T01
Gene Sequence

>MsG0680031866.01.T01

ATGTGCAGCATAGTATATCCTTTGCTATCTTCAACTCATTTTGTATCCCTTCTTTCCCTCACACTTTCCAAGTTATCTTCAAAGATTCATCCAAAGAGTAAGATAGAAGAAGCCATGGAAACATCTCATCCTCTCTCAATAGAAAGTTTTTCATATAGTTGGTTAGTAAACCTTAAACCATCATCACTAGTAGAAAGCCTTGATGGTTCTTCTTTTAGAACCTATCTTGATTCTTCTGATGAAGCTTCTTCCTTCATTGAAATGGATCCAAGAATGCCATCTTCTAGAAGATTTTTTATAAACTCACAAGATTTAAAATTTGACTTTCCAACCTTACAACAATCCCCTCTCACTACTCTTGTTGATGCAGATCAACTCTTTTCCAATGGTTACCTAATGCCATTATTTGTTGAATCATTGAATATAGAACCATATGAATATGATTCATCAAATTCTAATTCAAATTCAGATTCAAATTCAACCTTAGCTTCTTCCATATCACATGTAACAAATAAAGTGGTTCCTCGAGAAGATTCAAGAAGCCCTTCATTGAAAAGGTGTAGAACATTATCAAGGAGCATGTTTCAAAAGTATTTGAAATTTTTAAGGCCTTTGTGTAGAAGATTGAGGGGTCAAAAATCAGGATCAAAACATGAAAATGTTGTGAAAAGAACTCAATCAGTGAAGAATATTAGAGGAAATTATGGTGATTCTTCTCCAAGAATTAGTGTTGCATATTCCGCGGATGATTGGCGGCGCGTGTCATGTGATTCTGATTGTTCAATTTATGAAGCTGTACTTCATTGCAAAAGATCCATTGGTATGTAA

Protein sequence

>MsG0680031866.01.T01

MCSIVYPLLSSTHFVSLLSLTLSKLSSKIHPKSKIEEAMETSHPLSIESFSYSWLVNLKPSSLVESLDGSSFRTYLDSSDEASSFIEMDPRMPSSRRFFINSQDLKFDFPTLQQSPLTTLVDADQLFSNGYLMPLFVESLNIEPYEYDSSNSNSNSDSNSTLASSISHVTNKVVPREDSRSPSLKRCRTLSRSMFQKYLKFLRPLCRRLRGQKSGSKHENVVKRTQSVKNIRGNYGDSSPRISVAYSADDWRRVSCDSDCSIYEAVLHCKRSIGM*