AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039741.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039741.01.T01 MTR_7g078700 91.456 316 26 1 1 315 1 316 0.0 588
MsG0780039741.01.T01 MTR_8g028710 66.877 317 98 4 1 310 1 317 7.73e-150 425
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039741.01.T01 AT2G46080 59.223 309 125 1 1 308 1 309 2.13e-131 378
MsG0780039741.01.T01 AT1G01550 54.259 317 136 3 1 315 1 310 7.92e-118 343
MsG0780039741.01.T01 AT1G01550 54.259 317 136 3 1 315 1 310 7.92e-118 343
MsG0780039741.01.T01 AT4G01360 40.728 302 157 7 16 303 10 303 5.85e-63 203
MsG0780039741.01.T01 AT3G61500 36.413 184 104 5 1 181 2 175 2.10e-25 103
MsG0780039741.01.T01 AT3G61500 37.500 176 97 5 9 181 10 175 1.52e-24 101

Find 67 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTACAACACCTTGGATAAT+AGG 0.211183 7:+68494990 None:intergenic
TGAAAATTGTGGCAGCATTT+TGG 0.214121 7:-68495318 MsG0780039741.01.T01:CDS
TCCCATTTGGAAATCCTTTC+CGG 0.219881 7:-68495772 MsG0780039741.01.T01:CDS
ATCTTGTAAATGAGGAAATT+AGG 0.221961 7:-68495088 MsG0780039741.01.T01:CDS
CGGGCATGTTCTTCACTTGA+TGG 0.229319 7:-68495377 MsG0780039741.01.T01:CDS
TACAAAGAACTTGATGGATA+TGG 0.310252 7:-68495159 MsG0780039741.01.T01:CDS
TCTTGTAAATGAGGAAATTA+GGG 0.343932 7:-68495087 MsG0780039741.01.T01:CDS
GCTAACAACTTCCCTTGCTT+TGG 0.344238 7:+68495652 None:intergenic
CTAACAACTTCCCTTGCTTT+GGG 0.367324 7:+68495653 None:intergenic
GGAGATAACTTAGTGCCTTT+AGG 0.370904 7:+68495740 None:intergenic
GAAGCTCAAGGTCTGTCATC+AGG 0.377856 7:+68495570 None:intergenic
TTCTGTAGACCTTTAAAAGT+TGG 0.385917 7:+68495110 None:intergenic
CAGTGCACGTTGCACAATTT+AGG 0.392698 7:-68495425 MsG0780039741.01.T01:CDS
TTGAGCTTCCCGTGACTGAT+TGG 0.404922 7:-68495556 MsG0780039741.01.T01:CDS
CGCATGTGATTCTGTTTCAA+TGG 0.407433 7:+68494965 None:intergenic
CATGTTCTTCACTTGATGGT+TGG 0.420616 7:-68495373 MsG0780039741.01.T01:CDS
CTACAACACCTTGGATAATA+GGG 0.420907 7:+68494991 None:intergenic
CTTCCCTTGCTTTGGGAGTG+AGG 0.431412 7:+68495660 None:intergenic
AGGCTCTACAAAGAACTTGA+TGG 0.436345 7:-68495165 MsG0780039741.01.T01:CDS
TGAGCTTCCCGTGACTGATT+GGG 0.443038 7:-68495555 MsG0780039741.01.T01:CDS
GGGAAGTTGTTAGCTTGTCT+TGG 0.444407 7:-68495643 MsG0780039741.01.T01:CDS
ATCCGGAAAGGATTTCCAAA+TGG 0.445961 7:+68495770 None:intergenic
GTTCTTCACTTGATGGTTGG+AGG 0.446966 7:-68495370 MsG0780039741.01.T01:CDS
TGACTGATTGGGATGAGAAA+TGG 0.450338 7:-68495544 MsG0780039741.01.T01:CDS
ATGTGGCTGATGTGTACTCT+TGG 0.450899 7:-68495136 MsG0780039741.01.T01:CDS
GATGGTTGGAGGCAGCATAT+TGG 0.451764 7:-68495359 MsG0780039741.01.T01:CDS
TGCCTTTAGGTGCTATCATC+CGG 0.456937 7:+68495753 None:intergenic
TGAGTTATCACGTCTGAAGC+AGG 0.464721 7:-68495462 MsG0780039741.01.T01:CDS
TCCGGAAAGGATTTCCAAAT+GGG 0.470277 7:+68495771 None:intergenic
GATAATACTGCCAGCAGTTG+TGG 0.482994 7:+68495719 None:intergenic
TTGGATGACTTCGGCTATGC+AGG 0.483092 7:-68495624 MsG0780039741.01.T01:CDS
TCTACAGAATCTTGTAAATG+AGG 0.499811 7:-68495096 MsG0780039741.01.T01:CDS
TAAGTTATCTCCACAACTGC+TGG 0.506800 7:-68495729 MsG0780039741.01.T01:CDS
TGTGGCTGATGTGTACTCTT+GGG 0.507413 7:-68495135 MsG0780039741.01.T01:CDS
TAGCTTGTCTTGGATGACTT+CGG 0.520698 7:-68495633 MsG0780039741.01.T01:CDS
TTGAGGCAAATTTGGAAGAG+AGG 0.538254 7:-68495691 MsG0780039741.01.T01:CDS
CACGACCAGACAAGACCACT+TGG 0.539337 7:+68494861 None:intergenic
TAGATCTTCTACAAAGGAGG+TGG 0.548875 7:-68494893 MsG0780039741.01.T01:CDS
GCGAAGACTGTTGAGAAGCT+AGG 0.551535 7:-68494945 MsG0780039741.01.T01:CDS
ATTCACTGTATTGAACGAGT+TGG 0.558633 7:-68495045 MsG0780039741.01.T01:CDS
TTCTCATCCCAATCAGTCAC+GGG 0.564019 7:+68495548 None:intergenic
AAAACCTCACTCCCAAAGCA+AGG 0.570200 7:-68495664 MsG0780039741.01.T01:CDS
CATCAAGTGAAGAACATGCC+CGG 0.575410 7:+68495378 None:intergenic
CATCTAAATGAGTCGTCCAC+AGG 0.578682 7:-68495813 None:intergenic
AAGCTCAAGGTCTGTCATCA+GGG 0.582026 7:+68495571 None:intergenic
GAACTTGATGGATATGGATG+TGG 0.582073 7:-68495153 MsG0780039741.01.T01:CDS
AGCTGTTGATTTGTCTGCGA+GGG 0.583334 7:-68495021 MsG0780039741.01.T01:CDS
GAAAAGCTGATCTGATGAGA+AGG 0.586785 7:+68495400 None:intergenic
TTCCGGATGATAGCACCTAA+AGG 0.589047 7:-68495755 MsG0780039741.01.T01:CDS
TTTCTCATCCCAATCAGTCA+CGG 0.595413 7:+68495547 None:intergenic
ACGTGCACTGAAGTAAAAGA+TGG 0.598474 7:+68495438 None:intergenic
CAATGGTATCTACAACACCT+TGG 0.598786 7:+68494982 None:intergenic
GAAATGGATAGATGTGTACT+TGG 0.601770 7:-68495528 MsG0780039741.01.T01:CDS
AAGGACTAGATCTTCTACAA+AGG 0.602091 7:-68494899 MsG0780039741.01.T01:CDS
TTAGGTGCTATCATCCGGAA+AGG 0.602901 7:+68495758 None:intergenic
GAATTATACCCTATTATCCA+AGG 0.604461 7:-68494999 MsG0780039741.01.T01:CDS
GCAACAGAGAATTTCTCTCA+AGG 0.606168 7:-68494918 MsG0780039741.01.T01:CDS
AATCAGTCACGGGAAGCTCA+AGG 0.612150 7:+68495558 None:intergenic
GACTAGATCTTCTACAAAGG+AGG 0.614280 7:-68494896 MsG0780039741.01.T01:CDS
AAACCTCACTCCCAAAGCAA+GGG 0.622458 7:-68495663 MsG0780039741.01.T01:CDS
GAGTTATCACGTCTGAAGCA+GGG 0.623647 7:-68495461 MsG0780039741.01.T01:CDS
TGGGTCATCTGATCTTCCAA+AGG 0.624962 7:-68495285 MsG0780039741.01.T01:CDS
TCTTCTACAAAGGAGGTGGA+TGG 0.629826 7:-68494889 MsG0780039741.01.T01:CDS
AACCTGAGAATTGAAAATTG+TGG 0.634817 7:-68495329 MsG0780039741.01.T01:CDS
GCAAGCTATCTATGGAGTGA+AGG 0.656346 7:-68495231 MsG0780039741.01.T01:CDS
AATGATCTAGGTGGATCCTG+TGG 0.674397 7:+68495797 None:intergenic
AAGCTGTTGATTTGTCTGCG+AGG 0.756834 7:-68495022 MsG0780039741.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATCTTGTAAATGAGGAAATT+AGG - Chr7:68495578-68495597 MsG0780039741.01.T01:CDS 25.0%
! TCTTGTAAATGAGGAAATTA+GGG - Chr7:68495579-68495598 MsG0780039741.01.T01:CDS 25.0%
AACCTGAGAATTGAAAATTG+TGG - Chr7:68495337-68495356 MsG0780039741.01.T01:CDS 30.0%
AAGGTTAAAAAGTCAGCAAA+AGG - Chr7:68495400-68495419 MsG0780039741.01.T01:CDS 30.0%
AGGTTAAAAAGTCAGCAAAA+GGG - Chr7:68495401-68495420 MsG0780039741.01.T01:CDS 30.0%
GAATTATACCCTATTATCCA+AGG - Chr7:68495667-68495686 MsG0780039741.01.T01:CDS 30.0%
TCTACAGAATCTTGTAAATG+AGG - Chr7:68495570-68495589 MsG0780039741.01.T01:CDS 30.0%
! TACAAAGAACTTGATGGATA+TGG - Chr7:68495507-68495526 MsG0780039741.01.T01:CDS 30.0%
! TTCTGTAGACCTTTAAAAGT+TGG + Chr7:68495559-68495578 None:intergenic 30.0%
!! ATTAGGGTTAGATTTTCAAG+TGG - Chr7:68495595-68495614 MsG0780039741.01.T01:CDS 30.0%
!!! TTAGGGTTAGATTTTCAAGT+GGG - Chr7:68495596-68495615 MsG0780039741.01.T01:CDS 30.0%
!!! TTTGCTGACTTTTTAACCTT+TGG + Chr7:68495400-68495419 None:intergenic 30.0%
AAGGACTAGATCTTCTACAA+AGG - Chr7:68495767-68495786 MsG0780039741.01.T01:CDS 35.0%
AATGGGAAAAATGATCTAGG+TGG + Chr7:68494881-68494900 None:intergenic 35.0%
ATTCACTGTATTGAACGAGT+TGG - Chr7:68495621-68495640 MsG0780039741.01.T01:CDS 35.0%
CCAAATGGGAAAAATGATCT+AGG + Chr7:68494884-68494903 None:intergenic 35.0%
CTACAACACCTTGGATAATA+GGG + Chr7:68495678-68495697 None:intergenic 35.0%
GAAATGGATAGATGTGTACT+TGG - Chr7:68495138-68495157 MsG0780039741.01.T01:CDS 35.0%
TAAAAAGTCAGCAAAAGGGA+AGG - Chr7:68495405-68495424 MsG0780039741.01.T01:CDS 35.0%
TCTACAACACCTTGGATAAT+AGG + Chr7:68495679-68495698 None:intergenic 35.0%
! TGAAAATTGTGGCAGCATTT+TGG - Chr7:68495348-68495367 MsG0780039741.01.T01:CDS 35.0%
! TGCCACAATTTTCAATTCTC+AGG + Chr7:68495342-68495361 None:intergenic 35.0%
!! CCTAGATCATTTTTCCCATT+TGG - Chr7:68494881-68494900 MsG0780039741.01.T01:CDS 35.0%
!! GTTTTGATGCAAGCTATCTA+TGG - Chr7:68495427-68495446 MsG0780039741.01.T01:CDS 35.0%
!!! ATCTGCTTTTGAGGCAAATT+TGG - Chr7:68494967-68494986 MsG0780039741.01.T01:CDS 35.0%
ACGTGCACTGAAGTAAAAGA+TGG + Chr7:68495231-68495250 None:intergenic 40.0%
AGGCTCTACAAAGAACTTGA+TGG - Chr7:68495501-68495520 MsG0780039741.01.T01:CDS 40.0%
ATCCGGAAAGGATTTCCAAA+TGG + Chr7:68494899-68494918 None:intergenic 40.0%
CAATGGTATCTACAACACCT+TGG + Chr7:68495687-68495706 None:intergenic 40.0%
CGCATGTGATTCTGTTTCAA+TGG + Chr7:68495704-68495723 None:intergenic 40.0%
CTAACAACTTCCCTTGCTTT+GGG + Chr7:68495016-68495035 None:intergenic 40.0%
GAAAAGCTGATCTGATGAGA+AGG + Chr7:68495269-68495288 None:intergenic 40.0%
GACTAGATCTTCTACAAAGG+AGG - Chr7:68495770-68495789 MsG0780039741.01.T01:CDS 40.0%
GCAACAGAGAATTTCTCTCA+AGG - Chr7:68495748-68495767 MsG0780039741.01.T01:CDS 40.0%
GGAGATAACTTAGTGCCTTT+AGG + Chr7:68494929-68494948 None:intergenic 40.0%
TAAGTTATCTCCACAACTGC+TGG - Chr7:68494937-68494956 MsG0780039741.01.T01:CDS 40.0%
TAGATCTTCTACAAAGGAGG+TGG - Chr7:68495773-68495792 MsG0780039741.01.T01:CDS 40.0%
TCCCATTTGGAAATCCTTTC+CGG - Chr7:68494894-68494913 MsG0780039741.01.T01:CDS 40.0%
TCCGGAAAGGATTTCCAAAT+GGG + Chr7:68494898-68494917 None:intergenic 40.0%
TGACTGATTGGGATGAGAAA+TGG - Chr7:68495122-68495141 MsG0780039741.01.T01:CDS 40.0%
TTGAGGCAAATTTGGAAGAG+AGG - Chr7:68494975-68494994 MsG0780039741.01.T01:CDS 40.0%
TTTCTCATCCCAATCAGTCA+CGG + Chr7:68495122-68495141 None:intergenic 40.0%
! AGCATTTTGGATAATCTGGC+TGG - Chr7:68495361-68495380 MsG0780039741.01.T01:CDS 40.0%
! CATGTTCTTCACTTGATGGT+TGG - Chr7:68495293-68495312 MsG0780039741.01.T01:CDS 40.0%
! GAACTTGATGGATATGGATG+TGG - Chr7:68495513-68495532 MsG0780039741.01.T01:CDS 40.0%
! GCATTTTGGATAATCTGGCT+GGG - Chr7:68495362-68495381 MsG0780039741.01.T01:CDS 40.0%
! TAGCTTGTCTTGGATGACTT+CGG - Chr7:68495033-68495052 MsG0780039741.01.T01:CDS 40.0%
! TCTCATCAGATCAGCTTTTC+CGG - Chr7:68495269-68495288 MsG0780039741.01.T01:CDS 40.0%
! TCTTGGGCTCCAACTTTTAA+AGG - Chr7:68495547-68495566 MsG0780039741.01.T01:CDS 40.0%
! TGGCAGCATTTTGGATAATC+TGG - Chr7:68495357-68495376 MsG0780039741.01.T01:CDS 40.0%
!! GGCAGTATTATCTGCTTTTG+AGG - Chr7:68494958-68494977 MsG0780039741.01.T01:CDS 40.0%
!!! GTGTTTGCAACTGCTTTTTC+AGG - Chr7:68495481-68495500 MsG0780039741.01.T01:CDS 40.0%
AAAACCTCACTCCCAAAGCA+AGG - Chr7:68495002-68495021 MsG0780039741.01.T01:CDS 45.0%
AAACCTCACTCCCAAAGCAA+GGG - Chr7:68495003-68495022 MsG0780039741.01.T01:CDS 45.0%
AAGCTCAAGGTCTGTCATCA+GGG + Chr7:68495098-68495117 None:intergenic 45.0%
AATGATCTAGGTGGATCCTG+TGG + Chr7:68494872-68494891 None:intergenic 45.0%
ATGTGGCTGATGTGTACTCT+TGG - Chr7:68495530-68495549 MsG0780039741.01.T01:CDS 45.0%
CAGTGCACGTTGCACAATTT+AGG - Chr7:68495241-68495260 MsG0780039741.01.T01:CDS 45.0%
CATCAAGTGAAGAACATGCC+CGG + Chr7:68495291-68495310 None:intergenic 45.0%
GATAATACTGCCAGCAGTTG+TGG + Chr7:68494950-68494969 None:intergenic 45.0%
GCAAGCTATCTATGGAGTGA+AGG - Chr7:68495435-68495454 MsG0780039741.01.T01:CDS 45.0%
GCTAACAACTTCCCTTGCTT+TGG + Chr7:68495017-68495036 None:intergenic 45.0%
TCTTCTACAAAGGAGGTGGA+TGG - Chr7:68495777-68495796 MsG0780039741.01.T01:CDS 45.0%
TGGGTCATCTGATCTTCCAA+AGG - Chr7:68495381-68495400 MsG0780039741.01.T01:CDS 45.0%
TGTGGCTGATGTGTACTCTT+GGG - Chr7:68495531-68495550 MsG0780039741.01.T01:CDS 45.0%
TTCCGGATGATAGCACCTAA+AGG - Chr7:68494911-68494930 MsG0780039741.01.T01:CDS 45.0%
TTCTCATCCCAATCAGTCAC+GGG + Chr7:68495121-68495140 None:intergenic 45.0%
! CTCATCAGATCAGCTTTTCC+GGG - Chr7:68495270-68495289 MsG0780039741.01.T01:CDS 45.0%
! GAGTTATCACGTCTGAAGCA+GGG - Chr7:68495205-68495224 MsG0780039741.01.T01:CDS 45.0%
! GTTCTTCACTTGATGGTTGG+AGG - Chr7:68495296-68495315 MsG0780039741.01.T01:CDS 45.0%
! TGAGTTATCACGTCTGAAGC+AGG - Chr7:68495204-68495223 MsG0780039741.01.T01:CDS 45.0%
! TGCCTTTAGGTGCTATCATC+CGG + Chr7:68494916-68494935 None:intergenic 45.0%
! TTAGGTGCTATCATCCGGAA+AGG + Chr7:68494911-68494930 None:intergenic 45.0%
!! AAGCTGTTGATTTGTCTGCG+AGG - Chr7:68495644-68495663 MsG0780039741.01.T01:CDS 45.0%
!! AGCTGTTGATTTGTCTGCGA+GGG - Chr7:68495645-68495664 MsG0780039741.01.T01:CDS 45.0%
!! GGGAAGTTGTTAGCTTGTCT+TGG - Chr7:68495023-68495042 MsG0780039741.01.T01:CDS 45.0%
!!! CTTTTCCAAGTGGTCTTGTC+TGG - Chr7:68495800-68495819 MsG0780039741.01.T01:CDS 45.0%
AATCAGTCACGGGAAGCTCA+AGG + Chr7:68495111-68495130 None:intergenic 50.0%
CGGGCATGTTCTTCACTTGA+TGG - Chr7:68495289-68495308 MsG0780039741.01.T01:CDS 50.0%
GAAGCTCAAGGTCTGTCATC+AGG + Chr7:68495099-68495118 None:intergenic 50.0%
GATGGTTGGAGGCAGCATAT+TGG - Chr7:68495307-68495326 MsG0780039741.01.T01:CDS 50.0%
GCGAAGACTGTTGAGAAGCT+AGG - Chr7:68495721-68495740 MsG0780039741.01.T01:CDS 50.0%
TGAGCTTCCCGTGACTGATT+GGG - Chr7:68495111-68495130 MsG0780039741.01.T01:CDS 50.0%
TTGAGCTTCCCGTGACTGAT+TGG - Chr7:68495110-68495129 MsG0780039741.01.T01:CDS 50.0%
TTGGATGACTTCGGCTATGC+AGG - Chr7:68495042-68495061 MsG0780039741.01.T01:CDS 50.0%
! AGGTGGATGGCTTTTCCAAG+TGG - Chr7:68495790-68495809 MsG0780039741.01.T01:CDS 50.0%
CACGACCAGACAAGACCACT+TGG + Chr7:68495808-68495827 None:intergenic 55.0%
!! CTTCCCTTGCTTTGGGAGTG+AGG + Chr7:68495009-68495028 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 68494860 68495828 68494860 ID=MsG0780039741.01;Name=MsG0780039741.01
Chr7 mRNA 68494860 68495828 68494860 ID=MsG0780039741.01.T01;Parent=MsG0780039741.01;Name=MsG0780039741.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|322
Chr7 exon 68494860 68495828 68494860 ID=MsG0780039741.01.T01:exon:11981;Parent=MsG0780039741.01.T01
Chr7 CDS 68494860 68495828 68494860 ID=MsG0780039741.01.T01:cds;Parent=MsG0780039741.01.T01
Gene Sequence

>MsG0780039741.01.T01

ATGAGTCGTCCACAGGATCCACCTAGATCATTTTTCCCATTTGGAAATCCTTTCCGGATGATAGCACCTAAAGGCACTAAGTTATCTCCACAACTGCTGGCAGTATTATCTGCTTTTGAGGCAAATTTGGAAGAGAGGCTGAAAAACCTCACTCCCAAAGCAAGGGAAGTTGTTAGCTTGTCTTGGATGACTTCGGCTATGCAGGCACTTTGTGAAAGTCACAATGACATTAAAACCCTGATGACAGACCTTGAGCTTCCCGTGACTGATTGGGATGAGAAATGGATAGATGTGTACTTGGACATCAGTGTGAAGTTGCTAGACATATGCATTGCATTTAGCTCTGAGTTATCACGTCTGAAGCAGGGCCATCTTTTACTTCAGTGCACGTTGCACAATTTAGGCTCCTTCTCATCAGATCAGCTTTTCCGGGCATGTTCTTCACTTGATGGTTGGAGGCAGCATATTGGTTCTAAAAACCTGAGAATTGAAAATTGTGGCAGCATTTTGGATAATCTGGCTGGGTCATCTGATCTTCCAAAGGTTAAAAAGTCAGCAAAAGGGAAGGTTTTGATGCAAGCTATCTATGGAGTGAAGGTGCTGACAGTTTTTGTGTGCAGTGTGTTTGCAACTGCTTTTTCAGGCTCTACAAAGAACTTGATGGATATGGATGTGGCTGATGTGTACTCTTGGGCTCCAACTTTTAAAGGTCTACAGAATCTTGTAAATGAGGAAATTAGGGTTAGATTTTCAAGTGGGAGATTCACTGTATTGAACGAGTTGGAAGCTGTTGATTTGTCTGCGAGGGAATTATACCCTATTATCCAAGGTGTTGTAGATACCATTGAAACAGAATCACATGCGAAGACTGTTGAGAAGCTAGGCAGAGCAACAGAGAATTTCTCTCAAGGACTAGATCTTCTACAAAGGAGGTGGATGGCTTTTCCAAGTGGTCTTGTCTGGTCGTGA

Protein sequence

>MsG0780039741.01.T01

MSRPQDPPRSFFPFGNPFRMIAPKGTKLSPQLLAVLSAFEANLEERLKNLTPKAREVVSLSWMTSAMQALCESHNDIKTLMTDLELPVTDWDEKWIDVYLDISVKLLDICIAFSSELSRLKQGHLLLQCTLHNLGSFSSDQLFRACSSLDGWRQHIGSKNLRIENCGSILDNLAGSSDLPKVKKSAKGKVLMQAIYGVKVLTVFVCSVFATAFSGSTKNLMDMDVADVYSWAPTFKGLQNLVNEEIRVRFSSGRFTVLNELEAVDLSARELYPIIQGVVDTIETESHAKTVEKLGRATENFSQGLDLLQRRWMAFPSGLVWS*