AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0341030


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 25 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCTATGAAATCTAGTAAA+AGG 0.216994 2_4:-62441127 Msa0341030:CDS
CAAATGGTTCTGCTATTCTT+TGG 0.337879 2_4:-62440919 Msa0341030:CDS
TGCTATGAAATCTAGTAAAA+GGG 0.426684 2_4:-62441126 Msa0341030:CDS
ATATGGAAAACTAGCTGAAA+AGG 0.450116 2_4:-62441045 Msa0341030:intron
TTGCTGTCAAATAACTTGAC+AGG 0.465743 2_4:+62441020 None:intergenic
AAATCTAGTAAAAGGGGTAG+TGG 0.485823 2_4:-62441119 Msa0341030:CDS
TCAACTCCACCGTCCTTGAC+AGG 0.488859 2_4:-62441094 Msa0341030:CDS
GAAGACGAGTTGGTTCTGCC+TGG 0.494135 2_4:-62440978 Msa0341030:CDS
ATCGAAGACTTGCAGACCCC+AGG 0.499552 2_4:+62440960 None:intergenic
AAGACGAGTTGGTTCTGCCT+GGG 0.519388 2_4:-62440977 Msa0341030:CDS
TGTGGAGGCATGCGTGCAAA+TGG 0.541660 2_4:-62440935 Msa0341030:CDS
TTACAGCCATGGCATGGAAG+GGG 0.555613 2_4:-62441152 Msa0341030:intron
TTTACAGCCATGGCATGGAA+GGG 0.559234 2_4:-62441153 Msa0341030:intron
TGACAGCAAGAACTGAGCTA+AGG 0.577227 2_4:-62441006 Msa0341030:CDS
GCATATCCCCTTCCATGCCA+TGG 0.578330 2_4:+62441146 None:intergenic
TAAGGGCAGAGAAGACGAGT+TGG 0.578709 2_4:-62440988 Msa0341030:CDS
AGACGAGTTGGTTCTGCCTG+GGG 0.579184 2_4:-62440976 Msa0341030:CDS
GACAGCAAGAACTGAGCTAA+GGG 0.580972 2_4:-62441005 Msa0341030:CDS
TCTGCAAGTCTTCGATGCTG+TGG 0.581284 2_4:-62440953 Msa0341030:CDS
GCTATGAAATCTAGTAAAAG+GGG 0.597009 2_4:-62441125 Msa0341030:CDS
GCAAGTCTTCGATGCTGTGG+AGG 0.611353 2_4:-62440950 Msa0341030:CDS
CTCCACCGTCCTTGACAGGA+TGG 0.613835 2_4:-62441090 Msa0341030:intron
CATGACCATCCTGTCAAGGA+CGG 0.626465 2_4:+62441085 None:intergenic
CATGCATGACCATCCTGTCA+AGG 0.634099 2_4:+62441081 None:intergenic
GACCATCCTGTCAAGGACGG+TGG 0.653998 2_4:+62441088 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGATATAACATTATTTAA+GGG + chr2_4:62441232-62441251 None:intergenic 10.0%
!! TTATGATATAACATTATTTA+AGG + chr2_4:62441233-62441252 None:intergenic 10.0%
!! AAATTATGATATAACATTAA+GGG + chr2_4:62441296-62441315 None:intergenic 10.0%
!! AAAATTATGATATAACATTA+AGG + chr2_4:62441297-62441316 None:intergenic 10.0%
!! TATGATATAACATTATTTAA+GGG + chr2_4:62441232-62441251 None:intergenic 10.0%
!! TTATGATATAACATTATTTA+AGG + chr2_4:62441233-62441252 None:intergenic 10.0%
!! AAATTATGATATAACATTAA+GGG + chr2_4:62441296-62441315 None:intergenic 10.0%
!! AAAATTATGATATAACATTA+AGG + chr2_4:62441297-62441316 None:intergenic 10.0%
!!! GTTTGAAATTTAACATTCAA+TGG - chr2_4:62441166-62441185 Msa0341030:intron 20.0%
!!! GTTATATCATAAGTTTTTAG+TGG - chr2_4:62441241-62441260 Msa0341030:intron 20.0%
!!! GTTTGAAATTTAACATTCAA+TGG - chr2_4:62441166-62441185 Msa0341030:intron 20.0%
!!! GTTATATCATAAGTTTTTAG+TGG - chr2_4:62441241-62441260 Msa0341030:intron 20.0%
! AGTTGCATACATATTAGTAA+TGG + chr2_4:62441340-62441359 None:intergenic 25.0%
! ATGCTATGAAATCTAGTAAA+AGG - chr2_4:62441413-62441432 Msa0341030:intron 25.0%
! TGCTATGAAATCTAGTAAAA+GGG - chr2_4:62441414-62441433 Msa0341030:intron 25.0%
! AGTTGCATACATATTAGTAA+TGG + chr2_4:62441340-62441359 None:intergenic 25.0%
! ATGCTATGAAATCTAGTAAA+AGG - chr2_4:62441413-62441432 Msa0341030:intron 25.0%
! TGCTATGAAATCTAGTAAAA+GGG - chr2_4:62441414-62441433 Msa0341030:intron 25.0%
CTTCCATATTTGTGACAAAT+GGG + chr2_4:62441203-62441222 None:intergenic 30.0%
TGGTATGTTATCATGTTGAA+GGG + chr2_4:62441320-62441339 None:intergenic 30.0%
ATGGTATGTTATCATGTTGA+AGG + chr2_4:62441321-62441340 None:intergenic 30.0%
GCTATGAAATCTAGTAAAAG+GGG - chr2_4:62441415-62441434 Msa0341030:intron 30.0%
ATATGGAAAACTAGCTGAAA+AGG - chr2_4:62441495-62441514 Msa0341030:intron 30.0%
CTTCCATATTTGTGACAAAT+GGG + chr2_4:62441203-62441222 None:intergenic 30.0%
TGGTATGTTATCATGTTGAA+GGG + chr2_4:62441320-62441339 None:intergenic 30.0%
ATGGTATGTTATCATGTTGA+AGG + chr2_4:62441321-62441340 None:intergenic 30.0%
GCTATGAAATCTAGTAAAAG+GGG - chr2_4:62441415-62441434 Msa0341030:intron 30.0%
ATATGGAAAACTAGCTGAAA+AGG - chr2_4:62441495-62441514 Msa0341030:intron 30.0%
CAGTTGCATCACATATATAC+TGG - chr2_4:62441070-62441089 Msa0341030:intron 35.0%
TTTCCTCCAACTGAAACATT+AGG - chr2_4:62441115-62441134 Msa0341030:CDS 35.0%
TTCCTCCAACTGAAACATTA+GGG - chr2_4:62441116-62441135 Msa0341030:CDS 35.0%
CATATTTGTGACAAATGGGA+AGG + chr2_4:62441199-62441218 None:intergenic 35.0%
CTTCCCATTTGTCACAAATA+TGG - chr2_4:62441197-62441216 Msa0341030:intron 35.0%
CCTTCCATATTTGTGACAAA+TGG + chr2_4:62441204-62441223 None:intergenic 35.0%
CCATTTGTCACAAATATGGA+AGG - chr2_4:62441201-62441220 Msa0341030:intron 35.0%
!!! ATAAGTTTTTAGTGGCTCAC+TGG - chr2_4:62441249-62441268 Msa0341030:intron 35.0%
! CATCAACTATTTTACAGCCA+TGG - chr2_4:62441377-62441396 Msa0341030:intron 35.0%
AAATCTAGTAAAAGGGGTAG+TGG - chr2_4:62441421-62441440 Msa0341030:intron 35.0%
TTGCTGTCAAATAACTTGAC+AGG + chr2_4:62441523-62441542 None:intergenic 35.0%
! CAAATGGTTCTGCTATTCTT+TGG - chr2_4:62441621-62441640 Msa0341030:intron 35.0%
CAGTTGCATCACATATATAC+TGG - chr2_4:62441070-62441089 Msa0341030:intron 35.0%
TTTCCTCCAACTGAAACATT+AGG - chr2_4:62441115-62441134 Msa0341030:CDS 35.0%
TTCCTCCAACTGAAACATTA+GGG - chr2_4:62441116-62441135 Msa0341030:CDS 35.0%
CATATTTGTGACAAATGGGA+AGG + chr2_4:62441199-62441218 None:intergenic 35.0%
CTTCCCATTTGTCACAAATA+TGG - chr2_4:62441197-62441216 Msa0341030:intron 35.0%
CCTTCCATATTTGTGACAAA+TGG + chr2_4:62441204-62441223 None:intergenic 35.0%
CCATTTGTCACAAATATGGA+AGG - chr2_4:62441201-62441220 Msa0341030:intron 35.0%
!!! ATAAGTTTTTAGTGGCTCAC+TGG - chr2_4:62441249-62441268 Msa0341030:intron 35.0%
! CATCAACTATTTTACAGCCA+TGG - chr2_4:62441377-62441396 Msa0341030:intron 35.0%
AAATCTAGTAAAAGGGGTAG+TGG - chr2_4:62441421-62441440 Msa0341030:intron 35.0%
TTGCTGTCAAATAACTTGAC+AGG + chr2_4:62441523-62441542 None:intergenic 35.0%
! CAAATGGTTCTGCTATTCTT+TGG - chr2_4:62441621-62441640 Msa0341030:intron 35.0%
! CTGCTAAGCATTTTGTGAAG+AGG - chr2_4:62440912-62440931 Msa0341030:CDS 40.0%
TAACTCAATGCTTCCAAGCA+CGG - chr2_4:62440995-62441014 Msa0341030:CDS 40.0%
TCCTCCAACTGAAACATTAG+GGG - chr2_4:62441117-62441136 Msa0341030:CDS 40.0%
! CTCACTGGTTAAACAATGGA+AGG - chr2_4:62441264-62441283 Msa0341030:intron 40.0%
! ACTATTTTACAGCCATGGCA+TGG - chr2_4:62441382-62441401 Msa0341030:intron 40.0%
CATGGATTCTCATGCTGATA+TGG - chr2_4:62441478-62441497 Msa0341030:intron 40.0%
! CTGCTAAGCATTTTGTGAAG+AGG - chr2_4:62440912-62440931 Msa0341030:CDS 40.0%
TAACTCAATGCTTCCAAGCA+CGG - chr2_4:62440995-62441014 Msa0341030:CDS 40.0%
TCCTCCAACTGAAACATTAG+GGG - chr2_4:62441117-62441136 Msa0341030:CDS 40.0%
! CTCACTGGTTAAACAATGGA+AGG - chr2_4:62441264-62441283 Msa0341030:intron 40.0%
! ACTATTTTACAGCCATGGCA+TGG - chr2_4:62441382-62441401 Msa0341030:intron 40.0%
CATGGATTCTCATGCTGATA+TGG - chr2_4:62441478-62441497 Msa0341030:intron 40.0%
ATGCTTCCAAGCACGGAAAA+AGG - chr2_4:62441002-62441021 Msa0341030:CDS 45.0%
! CAAGTACCTTTTTCCGTGCT+TGG + chr2_4:62441011-62441030 None:intergenic 45.0%
TCCCCTAATGTTTCAGTTGG+AGG + chr2_4:62441121-62441140 None:intergenic 45.0%
GCATCCCCTAATGTTTCAGT+TGG + chr2_4:62441124-62441143 None:intergenic 45.0%
! GTGGCTCACTGGTTAAACAA+TGG - chr2_4:62441260-62441279 Msa0341030:intron 45.0%
! TTTTACAGCCATGGCATGGA+AGG - chr2_4:62441386-62441405 Msa0341030:intron 45.0%
TTTACAGCCATGGCATGGAA+GGG - chr2_4:62441387-62441406 Msa0341030:intron 45.0%
TGACAGCAAGAACTGAGCTA+AGG - chr2_4:62441534-62441553 Msa0341030:intron 45.0%
GACAGCAAGAACTGAGCTAA+GGG - chr2_4:62441535-62441554 Msa0341030:intron 45.0%
ATGCTTCCAAGCACGGAAAA+AGG - chr2_4:62441002-62441021 Msa0341030:CDS 45.0%
! CAAGTACCTTTTTCCGTGCT+TGG + chr2_4:62441011-62441030 None:intergenic 45.0%
TCCCCTAATGTTTCAGTTGG+AGG + chr2_4:62441121-62441140 None:intergenic 45.0%
GCATCCCCTAATGTTTCAGT+TGG + chr2_4:62441124-62441143 None:intergenic 45.0%
! GTGGCTCACTGGTTAAACAA+TGG - chr2_4:62441260-62441279 Msa0341030:intron 45.0%
! TTTTACAGCCATGGCATGGA+AGG - chr2_4:62441386-62441405 Msa0341030:intron 45.0%
TTTACAGCCATGGCATGGAA+GGG - chr2_4:62441387-62441406 Msa0341030:intron 45.0%
TGACAGCAAGAACTGAGCTA+AGG - chr2_4:62441534-62441553 Msa0341030:intron 45.0%
GACAGCAAGAACTGAGCTAA+GGG - chr2_4:62441535-62441554 Msa0341030:intron 45.0%
TTACAGCCATGGCATGGAAG+GGG - chr2_4:62441388-62441407 Msa0341030:intron 50.0%
CATGACCATCCTGTCAAGGA+CGG + chr2_4:62441458-62441477 None:intergenic 50.0%
CATGCATGACCATCCTGTCA+AGG + chr2_4:62441462-62441481 None:intergenic 50.0%
CTTGACAGGATGGTCATGCA+TGG - chr2_4:62441460-62441479 Msa0341030:intron 50.0%
TAAGGGCAGAGAAGACGAGT+TGG - chr2_4:62441552-62441571 Msa0341030:intron 50.0%
!! AAGACGAGTTGGTTCTGCCT+GGG - chr2_4:62441563-62441582 Msa0341030:intron 50.0%
TCTGCAAGTCTTCGATGCTG+TGG - chr2_4:62441587-62441606 Msa0341030:intron 50.0%
TTACAGCCATGGCATGGAAG+GGG - chr2_4:62441388-62441407 Msa0341030:intron 50.0%
CATGACCATCCTGTCAAGGA+CGG + chr2_4:62441458-62441477 None:intergenic 50.0%
CATGCATGACCATCCTGTCA+AGG + chr2_4:62441462-62441481 None:intergenic 50.0%
CTTGACAGGATGGTCATGCA+TGG - chr2_4:62441460-62441479 Msa0341030:intron 50.0%
TAAGGGCAGAGAAGACGAGT+TGG - chr2_4:62441552-62441571 Msa0341030:intron 50.0%
!! AAGACGAGTTGGTTCTGCCT+GGG - chr2_4:62441563-62441582 Msa0341030:intron 50.0%
TCTGCAAGTCTTCGATGCTG+TGG - chr2_4:62441587-62441606 Msa0341030:intron 50.0%
GCATATCCCCTTCCATGCCA+TGG + chr2_4:62441397-62441416 None:intergenic 55.0%
TCAACTCCACCGTCCTTGAC+AGG - chr2_4:62441446-62441465 Msa0341030:intron 55.0%
!! GAAGACGAGTTGGTTCTGCC+TGG - chr2_4:62441562-62441581 Msa0341030:intron 55.0%
!! AGACGAGTTGGTTCTGCCTG+GGG - chr2_4:62441564-62441583 Msa0341030:intron 55.0%
ATCGAAGACTTGCAGACCCC+AGG + chr2_4:62441583-62441602 None:intergenic 55.0%
GCAAGTCTTCGATGCTGTGG+AGG - chr2_4:62441590-62441609 Msa0341030:intron 55.0%
! TGTGGAGGCATGCGTGCAAA+TGG - chr2_4:62441605-62441624 Msa0341030:intron 55.0%
GCATATCCCCTTCCATGCCA+TGG + chr2_4:62441397-62441416 None:intergenic 55.0%
TCAACTCCACCGTCCTTGAC+AGG - chr2_4:62441446-62441465 Msa0341030:intron 55.0%
!! GAAGACGAGTTGGTTCTGCC+TGG - chr2_4:62441562-62441581 Msa0341030:intron 55.0%
!! AGACGAGTTGGTTCTGCCTG+GGG - chr2_4:62441564-62441583 Msa0341030:intron 55.0%
ATCGAAGACTTGCAGACCCC+AGG + chr2_4:62441583-62441602 None:intergenic 55.0%
GCAAGTCTTCGATGCTGTGG+AGG - chr2_4:62441590-62441609 Msa0341030:intron 55.0%
! TGTGGAGGCATGCGTGCAAA+TGG - chr2_4:62441605-62441624 Msa0341030:intron 55.0%
CTCCACCGTCCTTGACAGGA+TGG - chr2_4:62441450-62441469 Msa0341030:intron 60.0%
GACCATCCTGTCAAGGACGG+TGG + chr2_4:62441455-62441474 None:intergenic 60.0%
CTCCACCGTCCTTGACAGGA+TGG - chr2_4:62441450-62441469 Msa0341030:intron 60.0%
GACCATCCTGTCAAGGACGG+TGG + chr2_4:62441455-62441474 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 62440898 62441664 62440898 ID=Msa0341030;Name=Msa0341030
chr2_4 mRNA 62440898 62441664 62440898 ID=Msa0341030-mRNA-1;Parent=Msa0341030;Name=Msa0341030-mRNA-1;_AED=0.46;_eAED=0.47;_QI=0|0|0|1|1|1|3|0|79
chr2_4 exon 62441659 62441664 62441659 ID=Msa0341030-mRNA-1:exon:19895;Parent=Msa0341030-mRNA-1
chr2_4 exon 62441091 62441168 62441091 ID=Msa0341030-mRNA-1:exon:19894;Parent=Msa0341030-mRNA-1
chr2_4 exon 62440898 62441053 62440898 ID=Msa0341030-mRNA-1:exon:19893;Parent=Msa0341030-mRNA-1
chr2_4 CDS 62441659 62441664 62441659 ID=Msa0341030-mRNA-1:cds;Parent=Msa0341030-mRNA-1
chr2_4 CDS 62441091 62441168 62441091 ID=Msa0341030-mRNA-1:cds;Parent=Msa0341030-mRNA-1
chr2_4 CDS 62440898 62441053 62440898 ID=Msa0341030-mRNA-1:cds;Parent=Msa0341030-mRNA-1
Gene Sequence

>Msa0341030

ATGCAACCATGGCATGGAAGGGGATATGCTATGAAATCTAGTAAAAGGGGTAGTGGATTCAACTCCACCGTCCTTGACAGGATGCTGAAAAGGAACCTGTCAAGTTATTTGACAGCAAGAACTGAGCTAAGGGCAGAGAAGACGAGTTGGTTCTGCCTGGGGTCTGCAAGTCTTCGATGCTGTGGAGGCATGCGTGCAAATGGTTCTGCTATTCTTTGGTTAGTAGAGATTAGCTCTTGA

Protein sequence

>Msa0341030

MQPWHGRGYAMKSSKRGSGFNSTVLDRMLKRNLSSYLTARTELRAEKTSWFCLGSASLRCCGGMRANGSAILWLVEISS*