AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0517050


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0517050 MtrunA17_Chr3g0128201 88.889 135 5 1 1 125 59 193 7.90e-81 235
Msa0517050 MtrunA17_Chr5g0428861 76.119 134 22 1 1 124 59 192 8.73e-72 213
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0517050 AT1G33140.1 77.037 135 21 1 1 125 60 194 2.22e-71 212
Msa0517050 AT1G33120.1 77.037 135 21 1 1 125 60 194 2.22e-71 212
Msa0517050 AT4G10450.1 74.815 135 24 1 1 125 60 194 8.43e-70 208
Msa0517050 AT4G10450.2 72.897 107 19 1 1 97 60 166 9.13e-52 161

Find 36 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGATGCTTGGTTTGGTTCC+AGG 0.300629 3_4:-68572129 Msa0517050:CDS
GGTGAAGGATGAGGTTGTTT+TGG 0.319250 3_4:-68571512 Msa0517050:CDS
CTCAAGATCGATGCTTGGTT+TGG 0.321969 3_4:-68572136 None:intergenic
ATGACATTGAGCTTGTTTCT+AGG 0.326351 3_4:-68571483 Msa0517050:CDS
CTCAATCGACTTGTTGTCAT+TGG 0.358658 3_4:+68571982 None:intergenic
GGTATTTATGTTAGTGAGAA+GGG 0.433044 3_4:-68571301 Msa0517050:CDS
ATTGGTGATGCTAGCGTTGA+TGG 0.438976 3_4:+68572000 None:intergenic
CATGTTAAGAAGAAGGATAT+CGG 0.445187 3_4:-68571337 Msa0517050:CDS
CGATATCCTTCTTCTTAACA+TGG 0.447635 3_4:+68571338 None:intergenic
AGGAAAGTAGATTTGCTTGA+TGG 0.454716 3_4:-68571559 Msa0517050:CDS
AGCAGTTCTGATGGCGGCGG+AGG 0.457127 3_4:+68572102 None:intergenic
TTCTTCTCAAGATCGATGCT+TGG 0.472069 3_4:-68572141 None:intergenic
GATATCGGGAAGTTTCTTGA+TGG 0.479105 3_4:-68571322 Msa0517050:CDS
GAAATGCCATGTTAAGAAGA+AGG 0.499506 3_4:-68571344 Msa0517050:CDS
ATTTACTAGTTTGAGATATG+TGG 0.506984 3_4:-68571611 Msa0517050:intron
TTCAATTGTTCGATCTGAGA+AGG 0.526562 3_4:-68571533 Msa0517050:CDS
ATGTTAAGAAGAAGGATATC+GGG 0.529482 3_4:-68571336 Msa0517050:CDS
TGGTATTTATGTTAGTGAGA+AGG 0.531892 3_4:-68571302 Msa0517050:CDS
TTGGTGATGCTAGCGTTGAT+GGG 0.543746 3_4:+68572001 None:intergenic
TTATGTTAGTGAGAAGGGAT+CGG 0.552761 3_4:-68571296 Msa0517050:CDS
CACGTTGAGACTCTCATCAC+TGG 0.557442 3_4:-68572073 Msa0517050:CDS
CATCTTGTAGCGGAAACCCT+TGG 0.558729 3_4:+68572045 None:intergenic
AGGGTTTCCGCTACAAGATG+AGG 0.564650 3_4:-68572042 Msa0517050:CDS
ATCTGAGAAGGTGAAGGATG+AGG 0.567425 3_4:-68571521 Msa0517050:CDS
GAGAGCAGTTCTGATGGCGG+CGG 0.574544 3_4:+68572099 None:intergenic
CTCATCACTGGTGTTACCAA+GGG 0.576805 3_4:-68572061 Msa0517050:CDS
GTGGCTGAGAGCAGTTCTGA+TGG 0.581831 3_4:+68572093 None:intergenic
TCTCATCACTGGTGTTACCA+AGG 0.605297 3_4:-68572062 Msa0517050:CDS
GTCATGTGCACTTATTAACC+AGG 0.625933 3_4:-68571461 Msa0517050:intron
TGTTCGATCTGAGAAGGTGA+AGG 0.628133 3_4:-68571527 Msa0517050:CDS
TGTTAGTGAGAAGGGATCGG+TGG 0.651106 3_4:-68571293 Msa0517050:CDS
GGTGGTAACTGAAGAATAGA+CGG 0.652710 3_4:-68571275 Msa0517050:exon
TGGTGATGCTAGCGTTGATG+GGG 0.676063 3_4:+68572002 None:intergenic
GCTGAGAGCAGTTCTGATGG+CGG 0.677632 3_4:+68572096 None:intergenic
ACACAAACCTCATCTTGTAG+CGG 0.691692 3_4:+68572035 None:intergenic
CAGTGATGAGAGTCTCAACG+TGG 0.770839 3_4:+68572074 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTATTTCTTCAATTTT+AGG - chr3_4:68571500-68571519 Msa0517050:CDS 10.0%
!! TATAAATTAGATTAAAACTA+GGG + chr3_4:68571567-68571586 None:intergenic 10.0%
!! TTATAAATTAGATTAAAACT+AGG + chr3_4:68571568-68571587 None:intergenic 10.0%
!!! ATGAGTTAATTTTTTTATTT+TGG - chr3_4:68571710-68571729 Msa0517050:intron 10.0%
!!! ATTTTTATTTCTTCAATTTT+AGG - chr3_4:68571500-68571519 Msa0517050:CDS 10.0%
!! TATAAATTAGATTAAAACTA+GGG + chr3_4:68571567-68571586 None:intergenic 10.0%
!! TTATAAATTAGATTAAAACT+AGG + chr3_4:68571568-68571587 None:intergenic 10.0%
!!! ATGAGTTAATTTTTTTATTT+TGG - chr3_4:68571710-68571729 Msa0517050:intron 10.0%
!! TAAAATTGCATAAAACAACA+AGG + chr3_4:68571534-68571553 None:intergenic 20.0%
!! TAAAATTGCATAAAACAACA+AGG + chr3_4:68571534-68571553 None:intergenic 20.0%
!! ATTGAGATCTGAAACTTTAT+TGG - chr3_4:68571435-68571454 Msa0517050:intron 25.0%
! GAGATAATGTCTGATTTATT+TGG - chr3_4:68571744-68571763 Msa0517050:intron 25.0%
!! ATTTACTAGTTTGAGATATG+TGG - chr3_4:68571789-68571808 Msa0517050:intron 25.0%
! GAATCGAATAATGTTGTATT+AGG - chr3_4:68571816-68571835 Msa0517050:intron 25.0%
! ACAAAAATAAAGCATGTTCA+TGG + chr3_4:68571983-68572002 None:intergenic 25.0%
!! ATTGAGATCTGAAACTTTAT+TGG - chr3_4:68571435-68571454 Msa0517050:intron 25.0%
! GAGATAATGTCTGATTTATT+TGG - chr3_4:68571744-68571763 Msa0517050:intron 25.0%
!! ATTTACTAGTTTGAGATATG+TGG - chr3_4:68571789-68571808 Msa0517050:intron 25.0%
! GAATCGAATAATGTTGTATT+AGG - chr3_4:68571816-68571835 Msa0517050:intron 25.0%
! ACAAAAATAAAGCATGTTCA+TGG + chr3_4:68571983-68572002 None:intergenic 25.0%
AAACTTTATTGGAGAGAAGA+AGG - chr3_4:68571446-68571465 Msa0517050:intron 30.0%
GTCTGATTTATTTGGTTATC+TGG - chr3_4:68571752-68571771 Msa0517050:intron 30.0%
GAATAATGTTGTATTAGGTG+AGG - chr3_4:68571821-68571840 Msa0517050:intron 30.0%
!! GAATGTTGTTGTTGTGTTTT+TGG - chr3_4:68572029-68572048 Msa0517050:CDS 30.0%
CATGTTAAGAAGAAGGATAT+CGG - chr3_4:68572063-68572082 Msa0517050:CDS 30.0%
ATGTTAAGAAGAAGGATATC+GGG - chr3_4:68572064-68572083 Msa0517050:CDS 30.0%
TGGTATTTATGTTAGTGAGA+AGG - chr3_4:68572098-68572117 Msa0517050:CDS 30.0%
GGTATTTATGTTAGTGAGAA+GGG - chr3_4:68572099-68572118 Msa0517050:CDS 30.0%
AAACTTTATTGGAGAGAAGA+AGG - chr3_4:68571446-68571465 Msa0517050:intron 30.0%
GTCTGATTTATTTGGTTATC+TGG - chr3_4:68571752-68571771 Msa0517050:intron 30.0%
GAATAATGTTGTATTAGGTG+AGG - chr3_4:68571821-68571840 Msa0517050:intron 30.0%
!! GAATGTTGTTGTTGTGTTTT+TGG - chr3_4:68572029-68572048 Msa0517050:CDS 30.0%
CATGTTAAGAAGAAGGATAT+CGG - chr3_4:68572063-68572082 Msa0517050:CDS 30.0%
ATGTTAAGAAGAAGGATATC+GGG - chr3_4:68572064-68572083 Msa0517050:CDS 30.0%
TGGTATTTATGTTAGTGAGA+AGG - chr3_4:68572098-68572117 Msa0517050:CDS 30.0%
GGTATTTATGTTAGTGAGAA+GGG - chr3_4:68572099-68572118 Msa0517050:CDS 30.0%
! AGGAAAGTAGATTTGCTTGA+TGG - chr3_4:68571841-68571860 Msa0517050:intron 35.0%
TTCAATTGTTCGATCTGAGA+AGG - chr3_4:68571867-68571886 Msa0517050:intron 35.0%
! ATGACATTGAGCTTGTTTCT+AGG - chr3_4:68571917-68571936 Msa0517050:intron 35.0%
ATGTTCATGGACCATGTTAA+GGG + chr3_4:68571970-68571989 None:intergenic 35.0%
GAAATGCCATGTTAAGAAGA+AGG - chr3_4:68572056-68572075 Msa0517050:CDS 35.0%
CGATATCCTTCTTCTTAACA+TGG + chr3_4:68572065-68572084 None:intergenic 35.0%
TTATGTTAGTGAGAAGGGAT+CGG - chr3_4:68572104-68572123 Msa0517050:CDS 35.0%
! AGGAAAGTAGATTTGCTTGA+TGG - chr3_4:68571841-68571860 Msa0517050:intron 35.0%
TTCAATTGTTCGATCTGAGA+AGG - chr3_4:68571867-68571886 Msa0517050:intron 35.0%
! ATGACATTGAGCTTGTTTCT+AGG - chr3_4:68571917-68571936 Msa0517050:intron 35.0%
ATGTTCATGGACCATGTTAA+GGG + chr3_4:68571970-68571989 None:intergenic 35.0%
GAAATGCCATGTTAAGAAGA+AGG - chr3_4:68572056-68572075 Msa0517050:CDS 35.0%
CGATATCCTTCTTCTTAACA+TGG + chr3_4:68572065-68572084 None:intergenic 35.0%
TTATGTTAGTGAGAAGGGAT+CGG - chr3_4:68572104-68572123 Msa0517050:CDS 35.0%
ACACAAACCTCATCTTGTAG+CGG + chr3_4:68571368-68571387 None:intergenic 40.0%
!! CTCAATCGACTTGTTGTCAT+TGG + chr3_4:68571421-68571440 None:intergenic 40.0%
!!! AAGGATGAGGTTGTTTTGGA+TGG - chr3_4:68571892-68571911 Msa0517050:intron 40.0%
GTCATGTGCACTTATTAACC+AGG - chr3_4:68571939-68571958 Msa0517050:intron 40.0%
TGTTCATGGACCATGTTAAG+GGG + chr3_4:68571969-68571988 None:intergenic 40.0%
CATGTTCATGGACCATGTTA+AGG + chr3_4:68571971-68571990 None:intergenic 40.0%
GATATCGGGAAGTTTCTTGA+TGG - chr3_4:68572078-68572097 Msa0517050:CDS 40.0%
GGTGGTAACTGAAGAATAGA+CGG - chr3_4:68572125-68572144 Msa0517050:CDS 40.0%
ACACAAACCTCATCTTGTAG+CGG + chr3_4:68571368-68571387 None:intergenic 40.0%
!! CTCAATCGACTTGTTGTCAT+TGG + chr3_4:68571421-68571440 None:intergenic 40.0%
!!! AAGGATGAGGTTGTTTTGGA+TGG - chr3_4:68571892-68571911 Msa0517050:intron 40.0%
GTCATGTGCACTTATTAACC+AGG - chr3_4:68571939-68571958 Msa0517050:intron 40.0%
TGTTCATGGACCATGTTAAG+GGG + chr3_4:68571969-68571988 None:intergenic 40.0%
CATGTTCATGGACCATGTTA+AGG + chr3_4:68571971-68571990 None:intergenic 40.0%
GATATCGGGAAGTTTCTTGA+TGG - chr3_4:68572078-68572097 Msa0517050:CDS 40.0%
GGTGGTAACTGAAGAATAGA+CGG - chr3_4:68572125-68572144 Msa0517050:CDS 40.0%
!! TCTCATCACTGGTGTTACCA+AGG - chr3_4:68571338-68571357 Msa0517050:CDS 45.0%
!! CTCATCACTGGTGTTACCAA+GGG - chr3_4:68571339-68571358 Msa0517050:CDS 45.0%
!! TTGGTGATGCTAGCGTTGAT+GGG + chr3_4:68571402-68571421 None:intergenic 45.0%
!! ATTGGTGATGCTAGCGTTGA+TGG + chr3_4:68571403-68571422 None:intergenic 45.0%
TGTTCGATCTGAGAAGGTGA+AGG - chr3_4:68571873-68571892 Msa0517050:intron 45.0%
ATCTGAGAAGGTGAAGGATG+AGG - chr3_4:68571879-68571898 Msa0517050:intron 45.0%
! GGTGAAGGATGAGGTTGTTT+TGG - chr3_4:68571888-68571907 Msa0517050:intron 45.0%
!! TCTCATCACTGGTGTTACCA+AGG - chr3_4:68571338-68571357 Msa0517050:CDS 45.0%
!! CTCATCACTGGTGTTACCAA+GGG - chr3_4:68571339-68571358 Msa0517050:CDS 45.0%
!! TTGGTGATGCTAGCGTTGAT+GGG + chr3_4:68571402-68571421 None:intergenic 45.0%
!! ATTGGTGATGCTAGCGTTGA+TGG + chr3_4:68571403-68571422 None:intergenic 45.0%
TGTTCGATCTGAGAAGGTGA+AGG - chr3_4:68571873-68571892 Msa0517050:intron 45.0%
ATCTGAGAAGGTGAAGGATG+AGG - chr3_4:68571879-68571898 Msa0517050:intron 45.0%
! GGTGAAGGATGAGGTTGTTT+TGG - chr3_4:68571888-68571907 Msa0517050:intron 45.0%
!! TCGATGCTTGGTTTGGTTCC+AGG - chr3_4:68571271-68571290 Msa0517050:exon 50.0%
CAGTGATGAGAGTCTCAACG+TGG + chr3_4:68571329-68571348 None:intergenic 50.0%
CACGTTGAGACTCTCATCAC+TGG - chr3_4:68571327-68571346 Msa0517050:CDS 50.0%
! CATCTTGTAGCGGAAACCCT+TGG + chr3_4:68571358-68571377 None:intergenic 50.0%
AGGGTTTCCGCTACAAGATG+AGG - chr3_4:68571358-68571377 Msa0517050:intron 50.0%
!! TGGTGATGCTAGCGTTGATG+GGG + chr3_4:68571401-68571420 None:intergenic 50.0%
ACCAGGTACACCCCTTAACA+TGG - chr3_4:68571956-68571975 Msa0517050:intron 50.0%
! ACCATGTTAAGGGGTGTACC+TGG + chr3_4:68571960-68571979 None:intergenic 50.0%
TGTTAGTGAGAAGGGATCGG+TGG - chr3_4:68572107-68572126 Msa0517050:CDS 50.0%
!! TCGATGCTTGGTTTGGTTCC+AGG - chr3_4:68571271-68571290 Msa0517050:exon 50.0%
CAGTGATGAGAGTCTCAACG+TGG + chr3_4:68571329-68571348 None:intergenic 50.0%
CACGTTGAGACTCTCATCAC+TGG - chr3_4:68571327-68571346 Msa0517050:CDS 50.0%
! CATCTTGTAGCGGAAACCCT+TGG + chr3_4:68571358-68571377 None:intergenic 50.0%
AGGGTTTCCGCTACAAGATG+AGG - chr3_4:68571358-68571377 Msa0517050:intron 50.0%
!! TGGTGATGCTAGCGTTGATG+GGG + chr3_4:68571401-68571420 None:intergenic 50.0%
ACCAGGTACACCCCTTAACA+TGG - chr3_4:68571956-68571975 Msa0517050:intron 50.0%
! ACCATGTTAAGGGGTGTACC+TGG + chr3_4:68571960-68571979 None:intergenic 50.0%
TGTTAGTGAGAAGGGATCGG+TGG - chr3_4:68572107-68572126 Msa0517050:CDS 50.0%
! GCTGAGAGCAGTTCTGATGG+CGG + chr3_4:68571307-68571326 None:intergenic 55.0%
! GTGGCTGAGAGCAGTTCTGA+TGG + chr3_4:68571310-68571329 None:intergenic 55.0%
! GCTGAGAGCAGTTCTGATGG+CGG + chr3_4:68571307-68571326 None:intergenic 55.0%
! GTGGCTGAGAGCAGTTCTGA+TGG + chr3_4:68571310-68571329 None:intergenic 55.0%
!! GAGAGCAGTTCTGATGGCGG+CGG + chr3_4:68571304-68571323 None:intergenic 60.0%
!! GAGAGCAGTTCTGATGGCGG+CGG + chr3_4:68571304-68571323 None:intergenic 60.0%
!! GATGGCGGCGGAGGTTTTCC+TGG + chr3_4:68571292-68571311 None:intergenic 65.0%
!! AGCAGTTCTGATGGCGGCGG+AGG + chr3_4:68571301-68571320 None:intergenic 65.0%
!! GATGGCGGCGGAGGTTTTCC+TGG + chr3_4:68571292-68571311 None:intergenic 65.0%
!! AGCAGTTCTGATGGCGGCGG+AGG + chr3_4:68571301-68571320 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 68571270 68572152 68571270 ID=Msa0517050;Name=Msa0517050
chr3_4 mRNA 68571270 68572152 68571270 ID=Msa0517050-mRNA-1;Parent=Msa0517050;Name=Msa0517050-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|0.33|0.5|1|0.33|0.5|4|9|125
chr3_4 exon 68571270 68571365 68571270 ID=Msa0517050-mRNA-1:exon:18126;Parent=Msa0517050-mRNA-1
chr3_4 exon 68571462 68571584 68571462 ID=Msa0517050-mRNA-1:exon:18125;Parent=Msa0517050-mRNA-1
chr3_4 exon 68571612 68571618 68571612 ID=Msa0517050-mRNA-1:exon:18124;Parent=Msa0517050-mRNA-1
chr3_4 exon 68571992 68572152 68571992 ID=Msa0517050-mRNA-1:exon:18123;Parent=Msa0517050-mRNA-1
chr3_4 CDS 68571992 68572152 68571992 ID=Msa0517050-mRNA-1:cds;Parent=Msa0517050-mRNA-1
chr3_4 CDS 68571612 68571618 68571612 ID=Msa0517050-mRNA-1:cds;Parent=Msa0517050-mRNA-1
chr3_4 CDS 68571462 68571584 68571462 ID=Msa0517050-mRNA-1:cds;Parent=Msa0517050-mRNA-1
chr3_4 CDS 68571279 68571365 68571279 ID=Msa0517050-mRNA-1:cds;Parent=Msa0517050-mRNA-1
chr3_4 three_prime_UTR 68571270 68571278 68571270 ID=Msa0517050-mRNA-1:three_prime_utr;Parent=Msa0517050-mRNA-1
Gene Sequence

>Msa0517050

ATCGATGCTTGGTTTGGTTCCAGGAAAACCTCCGCCGCCATCAGAACTGCTCTCAGCCACGTTGAGACTCTCATCACTGGTGTTACCAAGGGTTTCCGCTACAAGATGAGGTTTGTGTATGCTCATTTCCCCATCAACGCTAGCATCACCAATGACAACAAATATGTGGTGAGGAAAGTAGATTTGCTTGATGGAGTTTCAATTGTTCGATCTGAGAAGGTGAAGGATGAGGTTGTTTTGGATGGAAATGACATTGAGCTTGTTTCTAGGTCATGTGCACTTATTAACCAGAAATGCCATGTTAAGAAGAAGGATATCGGGAAGTTTCTTGATGGTATTTATGTTAGTGAGAAGGGATCGGTGGTAACTGAAGAATAG

Protein sequence

>Msa0517050

IDAWFGSRKTSAAIRTALSHVETLITGVTKGFRYKMRFVYAHFPINASITNDNKYVVRKVDLLDGVSIVRSEKVKDEVVLDGNDIELVSRSCALINQKCHVKKKDIGKFLDGIYVSEKGSVVTEE*