Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0563830 | KEH20621.1 | 97.561 | 82 | 2 | 0 | 31 | 112 | 1 | 82 | 1.65e-48 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0563830 | sp|A0A1P8B760|AMI4G_ARATH | 69.444 | 72 | 21 | 1 | 30 | 100 | 436 | 507 | 3.91e-24 | 98.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0563830 | A0A072TTV0 | 97.561 | 82 | 2 | 0 | 31 | 112 | 1 | 82 | 7.89e-49 | 159 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0014710 | Msa0563830 | 0.813400 | 2.609610e-51 | -8.615850e-47 |
| Msa0017510 | Msa0563830 | 0.825075 | 5.713179e-54 | -8.615850e-47 |
| Msa0036140 | Msa0563830 | 0.811520 | 6.721203e-51 | -8.615850e-47 |
| Msa0045790 | Msa0563830 | 0.806943 | 6.433620e-50 | -8.615850e-47 |
| Msa0046160 | Msa0563830 | 0.806380 | 8.459765e-50 | -8.615850e-47 |
| Msa0068680 | Msa0563830 | 0.810005 | 1.428945e-50 | -8.615850e-47 |
| Msa0079400 | Msa0563830 | 0.811457 | 6.934901e-51 | -8.615850e-47 |
| Msa0095560 | Msa0563830 | 0.815998 | 6.936207e-52 | -8.615850e-47 |
| Msa0112750 | Msa0563830 | 0.805024 | 1.629956e-49 | -8.615850e-47 |
| Msa0122590 | Msa0563830 | 0.808635 | 2.810580e-50 | -8.615850e-47 |
| Msa0123300 | Msa0563830 | 0.817768 | 2.778841e-52 | -8.615850e-47 |
| Msa0157290 | Msa0563830 | 0.825308 | 5.031512e-54 | -8.615850e-47 |
| Msa0159770 | Msa0563830 | 0.806190 | 9.278259e-50 | -8.615850e-47 |
| Msa0166870 | Msa0563830 | 0.825187 | 5.373078e-54 | -8.615850e-47 |
| Msa0169860 | Msa0563830 | 0.801851 | 7.407104e-49 | -8.615850e-47 |
| Msa0170140 | Msa0563830 | 0.803816 | 2.909707e-49 | -8.615850e-47 |
| Msa0188830 | Msa0563830 | 0.813239 | 2.829938e-51 | -8.615850e-47 |
| Msa0210450 | Msa0563830 | 0.803291 | 3.738323e-49 | -8.615850e-47 |
| Msa0220580 | Msa0563830 | 0.822262 | 2.602515e-53 | -8.615850e-47 |
| Msa0223410 | Msa0563830 | -0.802107 | 6.563282e-49 | -8.615850e-47 |
| Msa0226890 | Msa0563830 | 0.814428 | 1.548259e-51 | -8.615850e-47 |
| Msa0237310 | Msa0563830 | 0.802994 | 4.308393e-49 | -8.615850e-47 |
| Msa0373450 | Msa0563830 | 0.806376 | 8.476181e-50 | -8.615850e-47 |
| Msa0373930 | Msa0563830 | 0.818662 | 1.743496e-52 | -8.615850e-47 |
| Msa0376350 | Msa0563830 | 0.820450 | 6.815050e-53 | -8.615850e-47 |
| Msa0376590 | Msa0563830 | 0.805610 | 1.228508e-49 | -8.615850e-47 |
| Msa0379640 | Msa0563830 | 0.827072 | 1.914615e-54 | -8.615850e-47 |
| Msa0388960 | Msa0563830 | 0.806862 | 6.693085e-50 | -8.615850e-47 |
| Msa0389340 | Msa0563830 | 0.817129 | 3.870126e-52 | -8.615850e-47 |
| Msa0402290 | Msa0563830 | 0.811747 | 5.998894e-51 | -8.615850e-47 |
| Msa0424790 | Msa0563830 | 0.806957 | 6.389972e-50 | -8.615850e-47 |
| Msa0431690 | Msa0563830 | 0.825910 | 3.622903e-54 | -8.615850e-47 |
| Msa0433410 | Msa0563830 | -0.804944 | 1.693941e-49 | -8.615850e-47 |
| Msa0439970 | Msa0563830 | 0.805858 | 1.089429e-49 | -8.615850e-47 |
| Msa0440750 | Msa0563830 | 0.802103 | 6.574487e-49 | -8.615850e-47 |
| Msa0455760 | Msa0563830 | 0.802619 | 5.148679e-49 | -8.615850e-47 |
| Msa0243100 | Msa0563830 | 0.818153 | 2.274209e-52 | -8.615850e-47 |
| Msa0243710 | Msa0563830 | 0.824540 | 7.635726e-54 | -8.615850e-47 |
| Msa0245940 | Msa0563830 | 0.811796 | 5.852931e-51 | -8.615850e-47 |
| Msa0270100 | Msa0563830 | 0.811346 | 7.331241e-51 | -8.615850e-47 |
| Msa0272730 | Msa0563830 | 0.808959 | 2.396892e-50 | -8.615850e-47 |
| Msa0274730 | Msa0563830 | 0.803276 | 3.766424e-49 | -8.615850e-47 |
| Msa0281640 | Msa0563830 | 0.803377 | 3.589015e-49 | -8.615850e-47 |
| Msa0284520 | Msa0563830 | 0.813651 | 2.298021e-51 | -8.615850e-47 |
| Msa0287410 | Msa0563830 | 0.807825 | 4.183395e-50 | -8.615850e-47 |
| Msa0287650 | Msa0563830 | 0.818614 | 1.787891e-52 | -8.615850e-47 |
| Msa0296560 | Msa0563830 | 0.800053 | 1.725793e-48 | -8.615850e-47 |
| Msa0315530 | Msa0563830 | 0.803617 | 3.200079e-49 | -8.615850e-47 |
| Msa0322690 | Msa0563830 | 0.817728 | 2.836884e-52 | -8.615850e-47 |
| Msa0326110 | Msa0563830 | 0.802660 | 5.048734e-49 | -8.615850e-47 |
| Msa0331000 | Msa0563830 | 0.800399 | 1.467926e-48 | -8.615850e-47 |
| Msa0340450 | Msa0563830 | 0.801655 | 8.127976e-49 | -8.615850e-47 |
| Msa0344910 | Msa0563830 | 0.802594 | 5.209950e-49 | -8.615850e-47 |
| Msa0351380 | Msa0563830 | 0.810860 | 9.343294e-51 | -8.615850e-47 |
| Msa0355220 | Msa0563830 | 0.803677 | 3.109555e-49 | -8.615850e-47 |
| Msa0484780 | Msa0563830 | 0.834934 | 2.248733e-56 | -8.615850e-47 |
| Msa0502890 | Msa0563830 | 0.806822 | 6.825676e-50 | -8.615850e-47 |
| Msa0512900 | Msa0563830 | 0.842367 | 2.699660e-58 | -8.615850e-47 |
| Msa0516170 | Msa0563830 | 0.825154 | 5.471289e-54 | -8.615850e-47 |
| Msa0516440 | Msa0563830 | 0.802446 | 5.589744e-49 | -8.615850e-47 |
| Msa0532350 | Msa0563830 | 0.814822 | 1.266804e-51 | -8.615850e-47 |
| Msa0536220 | Msa0563830 | 0.812583 | 3.941656e-51 | -8.615850e-47 |
| Msa0563830 | Msa0563840 | 0.821471 | 3.966723e-53 | -8.615850e-47 |
| Msa0563830 | Msa0577500 | 0.810978 | 8.807740e-51 | -8.615850e-47 |
| Msa0563830 | Msa0577510 | 0.883684 | 3.642914e-71 | -8.615850e-47 |
| Msa0563830 | Msa0579130 | 0.815609 | 8.469674e-52 | -8.615850e-47 |
| Msa0563830 | Msa0579200 | 0.806844 | 6.751890e-50 | -8.615850e-47 |
| Msa0563830 | Msa0599890 | 0.808770 | 2.630626e-50 | -8.615850e-47 |
| Msa0563830 | Msa0610430 | 0.801048 | 1.082295e-48 | -8.615850e-47 |
| Msa0563830 | Msa0629450 | 0.817311 | 3.522374e-52 | -8.615850e-47 |
| Msa0563830 | Msa0648880 | 0.807090 | 5.990608e-50 | -8.615850e-47 |
| Msa0563830 | Msa0651270 | 0.811877 | 5.620327e-51 | -8.615850e-47 |
| Msa0563830 | Msa0657750 | 0.806473 | 8.085534e-50 | -8.615850e-47 |
| Msa0563830 | Msa0657970 | 0.840268 | 9.630520e-58 | -8.615850e-47 |
| Msa0563830 | Msa0663240 | 0.805685 | 1.184547e-49 | -8.615850e-47 |
| Msa0563830 | Msa0667050 | 0.802446 | 5.587759e-49 | -8.615850e-47 |
| Msa0563830 | Msa0669440 | 0.805060 | 1.601991e-49 | -8.615850e-47 |
| Msa0563830 | Msa0673460 | 0.800944 | 1.136217e-48 | -8.615850e-47 |
| Msa0563830 | Msa0682550 | 0.815789 | 7.720900e-52 | -8.615850e-47 |
| Msa0563830 | Msa0685750 | 0.831157 | 1.957176e-55 | -8.615850e-47 |
| Msa0563830 | Msa0688070 | 0.833886 | 4.122115e-56 | -8.615850e-47 |
| Msa0563830 | Msa0693380 | 0.825446 | 4.666274e-54 | -8.615850e-47 |
| Msa0563830 | Msa0702350 | 0.806643 | 7.445449e-50 | -8.615850e-47 |
| Msa0563830 | Msa0702680 | 0.802164 | 6.389742e-49 | -8.615850e-47 |
| Msa0563830 | Msa0703050 | 0.805874 | 1.081040e-49 | -8.615850e-47 |
| Msa0563830 | Msa0711790 | 0.804874 | 1.751706e-49 | -8.615850e-47 |
| Msa0563830 | Msa0717890 | 0.800034 | 1.741378e-48 | -8.615850e-47 |
| Msa0563830 | Msa0723990 | 0.809005 | 2.342477e-50 | -8.615850e-47 |
| Msa0563830 | Msa0724210 | 0.801513 | 8.689112e-49 | -8.615850e-47 |
| Msa0563830 | Msa0731940 | 0.810359 | 1.198743e-50 | -8.615850e-47 |
| Msa0563830 | Msa0740510 | 0.809869 | 1.528513e-50 | -8.615850e-47 |
| Msa0563830 | Msa0760130 | 0.815402 | 9.418373e-52 | -8.615850e-47 |
| Msa0563830 | Msa0761910 | 0.815055 | 1.124593e-51 | -8.615850e-47 |
| Msa0563830 | Msa0764570 | 0.814793 | 1.285572e-51 | -8.615850e-47 |
| Msa0563830 | Msa0766630 | 0.808177 | 3.520223e-50 | -8.615850e-47 |
| Msa0563830 | Msa0767760 | 0.815578 | 8.606586e-52 | -8.615850e-47 |
| Msa0563830 | Msa0790510 | 0.806774 | 6.985468e-50 | -8.615850e-47 |
| Msa0563830 | Msa0810220 | 0.800344 | 1.506269e-48 | -8.615850e-47 |
| Msa0563830 | Msa0820770 | 0.816800 | 4.588243e-52 | -8.615850e-47 |
| Msa0563830 | Msa0830530 | 0.806911 | 6.535820e-50 | -8.615850e-47 |
| Msa0563830 | Msa0832340 | 0.806800 | 6.897662e-50 | -8.615850e-47 |
| Msa0563830 | Msa0861090 | 0.812871 | 3.408181e-51 | -8.615850e-47 |
| Msa0563830 | Msa0880140 | 0.822169 | 2.734421e-53 | -8.615850e-47 |
| Msa0563830 | Msa0924310 | 0.803827 | 2.894925e-49 | -8.615850e-47 |
| Msa0563830 | Msa0954550 | 0.801325 | 9.497576e-49 | -8.615850e-47 |
| Msa0563830 | Msa0969450 | 0.809827 | 1.560990e-50 | -8.615850e-47 |
| Msa0563830 | Msa0969520 | 0.845253 | 4.558535e-59 | -8.615850e-47 |
| Msa0563830 | Msa1014540 | 0.816469 | 5.443681e-52 | -8.615850e-47 |
| Msa0563830 | Msa1030970 | 0.815093 | 1.102894e-51 | -8.615850e-47 |
| Msa0563830 | Msa1075220 | -0.823124 | 1.639909e-53 | -8.615850e-47 |
| Msa0563830 | Msa1075540 | 0.809967 | 1.456039e-50 | -8.615850e-47 |
| Msa0563830 | Msa1081330 | 0.801706 | 7.935473e-49 | -8.615850e-47 |
| Msa0563830 | Msa1081590 | 0.806133 | 9.536627e-50 | -8.615850e-47 |
| Msa0563830 | Msa1082500 | 0.846504 | 2.084070e-59 | -8.615850e-47 |
| Msa0563830 | Msa1085200 | 0.804413 | 2.185898e-49 | -8.615850e-47 |
| Msa0563830 | Msa1090260 | 0.807802 | 4.229995e-50 | -8.615850e-47 |
| Msa0563830 | Msa1108160 | 0.806046 | 9.947833e-50 | -8.615850e-47 |
| Msa0563830 | Msa1116130 | -0.800284 | 1.548781e-48 | -8.615850e-47 |
| Msa0563830 | Msa1131220 | 0.811171 | 8.000411e-51 | -8.615850e-47 |
| Msa0563830 | Msa1162550 | -0.813837 | 2.091503e-51 | -8.615850e-47 |
| Msa0563830 | Msa1171910 | 0.804138 | 2.494379e-49 | -8.615850e-47 |
| Msa0563830 | Msa1224480 | 0.833701 | 4.585457e-56 | -8.615850e-47 |
| Msa0563830 | Msa1233890 | 0.808342 | 3.246675e-50 | -8.615850e-47 |
| Msa0563830 | Msa1239720 | 0.810248 | 1.266656e-50 | -8.615850e-47 |
| Msa0563830 | Msa1267610 | 0.805920 | 1.057613e-49 | -8.615850e-47 |
| Msa0563830 | Msa1278840 | 0.815035 | 1.136346e-51 | -8.615850e-47 |
| Msa0563830 | Msa1284680 | 0.830587 | 2.699919e-55 | -8.615850e-47 |
| Msa0563830 | Msa1297290 | 0.804174 | 2.452084e-49 | -8.615850e-47 |
| Msa0563830 | Msa1305340 | 0.800720 | 1.262789e-48 | -8.615850e-47 |
| Msa0563830 | Msa1306840 | 0.811920 | 5.500654e-51 | -8.615850e-47 |
| Msa0563830 | Msa1306850 | 0.913337 | 6.917826e-84 | -8.615850e-47 |
| Msa0563830 | Msa1314340 | 0.824946 | 6.125818e-54 | -8.615850e-47 |
| Msa0563830 | Msa1318990 | 0.819157 | 1.345938e-52 | -8.615850e-47 |
| Msa0563830 | Msa1322890 | 0.809030 | 2.314384e-50 | -8.615850e-47 |
| Msa0563830 | Msa1331000 | 0.814514 | 1.482012e-51 | -8.615850e-47 |
| Msa0563830 | Msa1333730 | 0.819788 | 9.663131e-53 | -8.615850e-47 |
| Msa0563830 | Msa1343760 | 0.808081 | 3.689715e-50 | -8.615850e-47 |
| Msa0563830 | Msa1357530 | 0.804682 | 1.921067e-49 | -8.615850e-47 |
| Msa0563830 | Msa1359040 | 0.808747 | 2.661107e-50 | -8.615850e-47 |
| Msa0563830 | Msa1360060 | 0.814018 | 1.907208e-51 | -8.615850e-47 |
| Msa0563830 | Msa1364830 | 0.815466 | 9.112921e-52 | -8.615850e-47 |
| Msa0563830 | Msa1372760 | 0.831502 | 1.610222e-55 | -8.615850e-47 |
| Msa0563830 | Msa1384350 | 0.804593 | 2.005634e-49 | -8.615850e-47 |
| Msa0563830 | Msa1385000 | 0.803233 | 3.844909e-49 | -8.615850e-47 |
| Msa0563830 | Msa1390050 | 0.822284 | 2.572577e-53 | -8.615850e-47 |
| Msa0563830 | Msa1392260 | 0.832796 | 7.704066e-56 | -8.615850e-47 |
| Msa0563830 | Msa1393040 | 0.814842 | 1.253658e-51 | -8.615850e-47 |
| Msa0563830 | Msa1397860 | 0.803140 | 4.017933e-49 | -8.615850e-47 |
| Msa0563830 | Msa1411020 | 0.820013 | 8.583669e-53 | -8.615850e-47 |
| Msa0563830 | Msa1424290 | 0.809721 | 1.645015e-50 | -8.615850e-47 |
| Msa0563830 | Msa1424680 | 0.812459 | 4.195992e-51 | -8.615850e-47 |
| Msa0563830 | Msa1429900 | 0.817298 | 3.544960e-52 | -8.615850e-47 |
| Msa0563830 | Msa1434350 | 0.810664 | 1.029987e-50 | -8.615850e-47 |
| Msa0563830 | Msa1436480 | 0.808700 | 2.722835e-50 | -8.615850e-47 |
| Msa0563830 | Msa1439320 | 0.814734 | 1.324571e-51 | -8.615850e-47 |
| Msa0563830 | Msa1440810 | 0.801915 | 7.186619e-49 | -8.615850e-47 |
| Msa0563830 | Msa1445030 | 0.813914 | 2.010458e-51 | -8.615850e-47 |
| Msa0563830 | Msa1451620 | -0.809434 | 1.895801e-50 | -8.615850e-47 |
| Msa0563830 | Msa1453560 | 0.828295 | 9.732249e-55 | -8.615850e-47 |
| Msa0563830 | Msa1464940 | 0.812921 | 3.324380e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0563830 | MtrunA17_Chr8g0377661 | 97.561 | 82 | 2 | 0 | 31 | 112 | 1 | 82 | 1.52e-52 | 159 |
| Msa0563830 | MtrunA17_Chr8g0377651 | 87.654 | 81 | 10 | 0 | 30 | 110 | 437 | 517 | 4.00e-42 | 144 |
| Msa0563830 | MtrunA17_Chr8g0377681 | 86.076 | 79 | 11 | 0 | 30 | 108 | 435 | 513 | 2.52e-41 | 142 |
| Msa0563830 | MtrunA17_Chr8g0377691 | 87.342 | 79 | 10 | 0 | 30 | 108 | 437 | 515 | 2.75e-41 | 142 |
| Msa0563830 | MtrunA17_Chr8g0377641 | 88.095 | 84 | 9 | 1 | 30 | 112 | 434 | 517 | 4.97e-41 | 141 |
| Msa0563830 | MtrunA17_Chr8g0377701 | 85.714 | 77 | 11 | 0 | 30 | 106 | 438 | 514 | 4.15e-37 | 130 |
| Msa0563830 | MtrunA17_Chr4g0037521 | 69.863 | 73 | 21 | 1 | 29 | 100 | 434 | 506 | 8.68e-26 | 100 |
| Msa0563830 | MtrunA17_Chr4g0037491 | 63.077 | 65 | 23 | 1 | 30 | 93 | 238 | 302 | 1.29e-20 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0563830 | AT4G34880.1 | 69.444 | 72 | 21 | 1 | 30 | 100 | 390 | 461 | 3.47e-25 | 98.6 |
| Msa0563830 | AT4G34880.2 | 69.444 | 72 | 21 | 1 | 30 | 100 | 436 | 507 | 3.97e-25 | 98.6 |
Find 28 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTCTGACGGTGTGGATTCT+TGG | 0.256519 | 4_1:-64293406 | Msa0563830:CDS |
| TTTAGTAGCATACTTGCTAT+TGG | 0.285041 | 4_1:-64293287 | Msa0563830:CDS |
| CTTGCTATTGGAGGTTATCT+TGG | 0.307365 | 4_1:-64293275 | Msa0563830:CDS |
| TTGGTCTTGGAAGCTTAGTT+TGG | 0.311340 | 4_1:-64293387 | Msa0563830:CDS |
| ATGCTACTAAAGAATGAAAA+AGG | 0.327148 | 4_1:+64293299 | None:intergenic |
| CAGCCATTTGGAATTTGCTT+TGG | 0.337732 | 4_1:-64293221 | Msa0563830:CDS |
| CCATTTGGAATTTGCTTTGG+AGG | 0.347630 | 4_1:-64293218 | Msa0563830:CDS |
| ACGGTGTGGATTCTTGGTCT+TGG | 0.417239 | 4_1:-64293400 | Msa0563830:CDS |
| GTTCCAGCAGGATATGAAAA+AGG | 0.427483 | 4_1:-64293245 | Msa0563830:CDS |
| GTCTTGGAAGCTTAGTTTGG+AGG | 0.439009 | 4_1:-64293384 | Msa0563830:CDS |
| TTGTAACTTCCAACTTCCTA+AGG | 0.444018 | 4_1:+64293117 | None:intergenic |
| TAAGCAATCTCAATCAACTT+TGG | 0.456653 | 4_1:+64293179 | None:intergenic |
| AATCCACACCGTCAGACAAC+GGG | 0.474967 | 4_1:+64293411 | None:intergenic |
| GCCATCACCCCTTAGGAAGT+TGG | 0.476560 | 4_1:-64293126 | Msa0563830:CDS |
| ATTTGCTTTGGAGGTTTGAA+AGG | 0.485064 | 4_1:-64293209 | Msa0563830:CDS |
| AGTAGCATACTTGCTATTGG+AGG | 0.497454 | 4_1:-64293284 | Msa0563830:CDS |
| TCAGAAAGCCATCACCCCTT+AGG | 0.498171 | 4_1:-64293133 | Msa0563830:CDS |
| TCTTGGAAGCTTAGTTTGGA+GGG | 0.501942 | 4_1:-64293383 | Msa0563830:CDS |
| CTTGGAGTGATTGTTCCAGC+AGG | 0.513725 | 4_1:-64293257 | Msa0563830:CDS |
| TCCAACTTCCTAAGGGGTGA+TGG | 0.529966 | 4_1:+64293125 | None:intergenic |
| CCTCCAAAGCAAATTCCAAA+TGG | 0.542753 | 4_1:+64293218 | None:intergenic |
| TGTAACTTCCAACTTCCTAA+GGG | 0.549765 | 4_1:+64293118 | None:intergenic |
| GGCGAGTGCCCGTTGTCTGA+CGG | 0.556589 | 4_1:-64293419 | Msa0563830:CDS |
| TTTGCTTTGGAGGTTTGAAA+GGG | 0.567329 | 4_1:-64293208 | Msa0563830:CDS |
| AATAAATAAACTTGATGCTG+TGG | 0.588874 | 4_1:-64293327 | Msa0563830:CDS |
| GAATCCACACCGTCAGACAA+CGG | 0.634170 | 4_1:+64293410 | None:intergenic |
| GTGCCCGTTGTCTGACGGTG+TGG | 0.634476 | 4_1:-64293414 | Msa0563830:CDS |
| GTAACTTCCAACTTCCTAAG+GGG | 0.639313 | 4_1:+64293119 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATAAATAAACTTGATGCTG+TGG | - | chr4_1:64293201-64293220 | Msa0563830:CDS | 25.0% |
| ! | ATGCTACTAAAGAATGAAAA+AGG | + | chr4_1:64293232-64293251 | None:intergenic | 25.0% |
| !! | TTTAGTAGCATACTTGCTAT+TGG | - | chr4_1:64293241-64293260 | Msa0563830:CDS | 30.0% |
| TAAGCAATCTCAATCAACTT+TGG | + | chr4_1:64293352-64293371 | None:intergenic | 30.0% | |
| TATGAAAAAGGTCAGCCATT+TGG | - | chr4_1:64293295-64293314 | Msa0563830:CDS | 35.0% | |
| !! | ATTTGCTTTGGAGGTTTGAA+AGG | - | chr4_1:64293319-64293338 | Msa0563830:CDS | 35.0% |
| !! | TTTGCTTTGGAGGTTTGAAA+GGG | - | chr4_1:64293320-64293339 | Msa0563830:CDS | 35.0% |
| TGTAACTTCCAACTTCCTAA+GGG | + | chr4_1:64293413-64293432 | None:intergenic | 35.0% | |
| TTGTAACTTCCAACTTCCTA+AGG | + | chr4_1:64293414-64293433 | None:intergenic | 35.0% | |
| !! | TTGGTCTTGGAAGCTTAGTT+TGG | - | chr4_1:64293141-64293160 | Msa0563830:CDS | 40.0% |
| ! | TCTTGGAAGCTTAGTTTGGA+GGG | - | chr4_1:64293145-64293164 | Msa0563830:CDS | 40.0% |
| !! | AGTAGCATACTTGCTATTGG+AGG | - | chr4_1:64293244-64293263 | Msa0563830:CDS | 40.0% |
| !! | CTTGCTATTGGAGGTTATCT+TGG | - | chr4_1:64293253-64293272 | Msa0563830:CDS | 40.0% |
| GTTCCAGCAGGATATGAAAA+AGG | - | chr4_1:64293283-64293302 | Msa0563830:CDS | 40.0% | |
| !! | TGACCTTTTTCATATCCTGC+TGG | + | chr4_1:64293289-64293308 | None:intergenic | 40.0% |
| ! | CAGCCATTTGGAATTTGCTT+TGG | - | chr4_1:64293307-64293326 | Msa0563830:CDS | 40.0% |
| CCTCCAAAGCAAATTCCAAA+TGG | + | chr4_1:64293313-64293332 | None:intergenic | 40.0% | |
| ! | CCATTTGGAATTTGCTTTGG+AGG | - | chr4_1:64293310-64293329 | Msa0563830:CDS | 40.0% |
| GTAACTTCCAACTTCCTAAG+GGG | + | chr4_1:64293412-64293431 | None:intergenic | 40.0% | |
| ! | GTCTTGGAAGCTTAGTTTGG+AGG | - | chr4_1:64293144-64293163 | Msa0563830:CDS | 45.0% |
| AATCCACACCGTCAGACAAC+GGG | + | chr4_1:64293120-64293139 | None:intergenic | 50.0% | |
| GAATCCACACCGTCAGACAA+CGG | + | chr4_1:64293121-64293140 | None:intergenic | 50.0% | |
| TGTCTGACGGTGTGGATTCT+TGG | - | chr4_1:64293122-64293141 | Msa0563830:CDS | 50.0% | |
| ACGGTGTGGATTCTTGGTCT+TGG | - | chr4_1:64293128-64293147 | Msa0563830:CDS | 50.0% | |
| CTTGGAGTGATTGTTCCAGC+AGG | - | chr4_1:64293271-64293290 | Msa0563830:CDS | 50.0% | |
| TCAGAAAGCCATCACCCCTT+AGG | - | chr4_1:64293395-64293414 | Msa0563830:CDS | 50.0% | |
| TCCAACTTCCTAAGGGGTGA+TGG | + | chr4_1:64293406-64293425 | None:intergenic | 50.0% | |
| GCCATCACCCCTTAGGAAGT+TGG | - | chr4_1:64293402-64293421 | Msa0563830:CDS | 55.0% | |
| GGCGAGTGCCCGTTGTCTGA+CGG | - | chr4_1:64293109-64293128 | Msa0563830:CDS | 65.0% | |
| GTGCCCGTTGTCTGACGGTG+TGG | - | chr4_1:64293114-64293133 | Msa0563830:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 64293106 | 64293444 | 64293106 | ID=Msa0563830;Name=Msa0563830 |
| chr4_1 | mRNA | 64293106 | 64293444 | 64293106 | ID=Msa0563830-mRNA-1;Parent=Msa0563830;Name=Msa0563830-mRNA-1;_AED=0.59;_eAED=0.59;_QI=0|-1|0|1|-1|1|1|0|112 |
| chr4_1 | exon | 64293106 | 64293444 | 64293106 | ID=Msa0563830-mRNA-1:exon:20588;Parent=Msa0563830-mRNA-1 |
| chr4_1 | CDS | 64293106 | 64293444 | 64293106 | ID=Msa0563830-mRNA-1:cds;Parent=Msa0563830-mRNA-1 |
| Gene Sequence |
| Protein sequence |
