AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1241190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1241190 MtrunA17_Chr8g0353881 98.913 92 1 0 1 92 29 120 4.41e-67 197
Msa1241190 MtrunA17_Chr3g0081741 91.304 92 8 0 1 92 29 120 8.17e-64 189
Msa1241190 MtrunA17_Chr3g0081771 78.571 56 12 0 30 85 8 63 1.61e-28 98.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1241190 AT3G02080.1 82.609 92 16 0 1 92 29 120 1.47e-58 176
Msa1241190 AT5G15520.1 81.522 92 17 0 1 92 29 120 2.00e-57 173
Msa1241190 AT5G61170.1 79.348 92 19 0 1 92 29 120 2.60e-57 173

Find 28 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTCATTCCAAAGGATTTA+TGG 0.171916 8_2:-24558625 Msa1241190:CDS
AACTGCAAAATTCAAAGAAT+TGG 0.319374 8_2:-24558850 Msa1241190:CDS
ATAATACCAATCAGGATCAT+AGG 0.347366 8_2:+24558824 None:intergenic
GGCTCCCACCATAAATCCTT+TGG 0.389483 8_2:+24558617 None:intergenic
ATCTACATAAGAGGCGGTCT+TGG 0.403127 8_2:-24558652 Msa1241190:CDS
GTACTCAATTTCAGCTTCCA+TGG 0.471471 8_2:-24558683 Msa1241190:intron
TGGCTCCCTATGATCCTGAT+TGG 0.474063 8_2:-24558830 Msa1241190:CDS
GCTCTAACATAATACCAATC+AGG 0.489642 8_2:+24558816 None:intergenic
TTGGTGTTGGTTCATTCCAA+AGG 0.500013 8_2:-24558633 Msa1241190:CDS
TATGTAGATCTTCCTTGCCA+TGG 0.510578 8_2:+24558666 None:intergenic
GAGGGCGACTTCCATTCCTC+TGG 0.516528 8_2:+24558596 None:intergenic
ATAAGAGGCGGTCTTGGTGT+TGG 0.519137 8_2:-24558646 Msa1241190:CDS
TAATACCAATCAGGATCATA+GGG 0.537599 8_2:+24558825 None:intergenic
CAATTTCAGCTTCCATGGCA+AGG 0.544710 8_2:-24558678 Msa1241190:intron
GCAAGGAAGATCTACATAAG+AGG 0.545628 8_2:-24558661 Msa1241190:CDS
TCGTCACATTCTTCAACAGT+TGG 0.571334 8_2:-24558542 Msa1241190:CDS
CATTCCAAAGGATTTATGGT+GGG 0.572274 8_2:-24558621 Msa1241190:CDS
GGATTTATGGTGGGAGCCAG+AGG 0.572742 8_2:-24558612 Msa1241190:CDS
GATTGGTATTATGTTAGAGC+TGG 0.573765 8_2:-24558813 Msa1241190:intron
GAACATGAACCTCATCGAGA+TGG 0.583253 8_2:-24558518 Msa1241190:CDS
TCATCGAGATGGATACCAAA+GGG 0.584221 8_2:-24558507 Msa1241190:CDS
TATGGTGGGAGCCAGAGGAA+TGG 0.616028 8_2:-24558607 Msa1241190:CDS
TCATTCCAAAGGATTTATGG+TGG 0.619819 8_2:-24558622 Msa1241190:CDS
TTGACAAGATCAGTCCACTC+AGG 0.623178 8_2:+24558873 None:intergenic
AATAGTTGGAGCTGCCTGAG+TGG 0.627148 8_2:-24558887 None:intergenic
CTCATCGAGATGGATACCAA+AGG 0.653842 8_2:-24558508 Msa1241190:CDS
CTTTGGTATCCATCTCGATG+AGG 0.659041 8_2:+24558509 None:intergenic
AGGAAGATCTACATAAGAGG+CGG 0.678529 8_2:-24558658 Msa1241190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AACTGCAAAATTCAAAGAAT+TGG - chr8_2:24558536-24558555 Msa1241190:CDS 25.0%
! AACATGATACTTCTAATTCT+TGG - chr8_2:24558639-24558658 Msa1241190:CDS 25.0%
TAATACCAATCAGGATCATA+GGG + chr8_2:24558564-24558583 None:intergenic 30.0%
ATAATACCAATCAGGATCAT+AGG + chr8_2:24558565-24558584 None:intergenic 30.0%
GCTCTAACATAATACCAATC+AGG + chr8_2:24558573-24558592 None:intergenic 35.0%
GATTGGTATTATGTTAGAGC+TGG - chr8_2:24558573-24558592 Msa1241190:CDS 35.0%
! TCTTGGTATTTTGTTGCTGT+TGG - chr8_2:24558656-24558675 Msa1241190:CDS 35.0%
GGTTCATTCCAAAGGATTTA+TGG - chr8_2:24558761-24558780 Msa1241190:intron 35.0%
TCATTCCAAAGGATTTATGG+TGG - chr8_2:24558764-24558783 Msa1241190:intron 35.0%
CATTCCAAAGGATTTATGGT+GGG - chr8_2:24558765-24558784 Msa1241190:intron 35.0%
GTACTCAATTTCAGCTTCCA+TGG - chr8_2:24558703-24558722 Msa1241190:intron 40.0%
TATGTAGATCTTCCTTGCCA+TGG + chr8_2:24558723-24558742 None:intergenic 40.0%
GCAAGGAAGATCTACATAAG+AGG - chr8_2:24558725-24558744 Msa1241190:intron 40.0%
AGGAAGATCTACATAAGAGG+CGG - chr8_2:24558728-24558747 Msa1241190:intron 40.0%
!! TTGGTGTTGGTTCATTCCAA+AGG - chr8_2:24558753-24558772 Msa1241190:intron 40.0%
TGCTCTTGCAAAAATGTGGA+GGG + chr8_2:24558811-24558830 None:intergenic 40.0%
TCGTCACATTCTTCAACAGT+TGG - chr8_2:24558844-24558863 Msa1241190:CDS 40.0%
TCATCGAGATGGATACCAAA+GGG - chr8_2:24558879-24558898 Msa1241190:CDS 40.0%
TTGACAAGATCAGTCCACTC+AGG + chr8_2:24558516-24558535 None:intergenic 45.0%
CAATTTCAGCTTCCATGGCA+AGG - chr8_2:24558708-24558727 Msa1241190:intron 45.0%
ATCTACATAAGAGGCGGTCT+TGG - chr8_2:24558734-24558753 Msa1241190:intron 45.0%
CTGCTCTTGCAAAAATGTGG+AGG + chr8_2:24558812-24558831 None:intergenic 45.0%
CCACTGCTCTTGCAAAAATG+TGG + chr8_2:24558815-24558834 None:intergenic 45.0%
! CCACATTTTTGCAAGAGCAG+TGG - chr8_2:24558812-24558831 Msa1241190:intron 45.0%
GAACATGAACCTCATCGAGA+TGG - chr8_2:24558868-24558887 Msa1241190:CDS 45.0%
CTTTGGTATCCATCTCGATG+AGG + chr8_2:24558880-24558899 None:intergenic 45.0%
CTCATCGAGATGGATACCAA+AGG - chr8_2:24558878-24558897 Msa1241190:CDS 45.0%
TGGCTCCCTATGATCCTGAT+TGG - chr8_2:24558556-24558575 Msa1241190:CDS 50.0%
! ATAAGAGGCGGTCTTGGTGT+TGG - chr8_2:24558740-24558759 Msa1241190:intron 50.0%
GGCTCCCACCATAAATCCTT+TGG + chr8_2:24558772-24558791 None:intergenic 50.0%
! GGATTTATGGTGGGAGCCAG+AGG - chr8_2:24558774-24558793 Msa1241190:intron 55.0%
! TATGGTGGGAGCCAGAGGAA+TGG - chr8_2:24558779-24558798 Msa1241190:intron 55.0%
GAGGGCGACTTCCATTCCTC+TGG + chr8_2:24558793-24558812 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 24558504 24558904 24558504 ID=Msa1241190;Name=Msa1241190
chr8_2 mRNA 24558504 24558904 24558504 ID=Msa1241190-mRNA-1;Parent=Msa1241190;Name=Msa1241190-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|1|0|1|1|1|2|0|92
chr8_2 exon 24558814 24558904 24558814 ID=Msa1241190-mRNA-1:exon:8687;Parent=Msa1241190-mRNA-1
chr8_2 exon 24558504 24558691 24558504 ID=Msa1241190-mRNA-1:exon:8686;Parent=Msa1241190-mRNA-1
chr8_2 CDS 24558814 24558904 24558814 ID=Msa1241190-mRNA-1:cds;Parent=Msa1241190-mRNA-1
chr8_2 CDS 24558504 24558691 24558504 ID=Msa1241190-mRNA-1:cds;Parent=Msa1241190-mRNA-1
Gene Sequence

>Msa1241190

TTGGAGCTGCCTGAGTGGACTGATCTTGTCAAAACTGCAAAATTCAAAGAATTGGCTCCCTATGATCCTGATTGGTATTATGTTAGAGCTGCTTCCATGGCAAGGAAGATCTACATAAGAGGCGGTCTTGGTGTTGGTTCATTCCAAAGGATTTATGGTGGGAGCCAGAGGAATGGAAGTCGCCCTCCACATTTTTGCAAGAGCAGTGGTGCTATTGCTCGTCACATTCTTCAACAGTTGGAGAACATGAACCTCATCGAGATGGATACCAAAGGGTAG

Protein sequence

>Msa1241190

LELPEWTDLVKTAKFKELAPYDPDWYYVRAASMARKIYIRGGLGVGSFQRIYGGSQRNGSRPPHFCKSSGAIARHILQQLENMNLIEMDTKG*