AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0059950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0059950 MtrunA17_Chr1g0159481 99.535 215 1 0 1 215 1 215 1.23e-160 442
Msa0059950 MtrunA17_Chr8g0384311 85.990 207 28 1 7 213 3 208 2.77e-133 372
Msa0059950 MtrunA17_Chr3g0130271 83.092 207 33 2 8 213 3 208 4.74e-126 354
Msa0059950 MtrunA17_Chr7g0265001 67.327 202 65 1 14 214 13 214 9.48e-104 298
Msa0059950 MtrunA17_Chr1g0180291 66.500 200 67 0 14 213 11 210 3.10e-102 294
Msa0059950 MtrunA17_Chr1g0197331 61.215 214 79 2 3 214 1 212 1.56e-91 267
Msa0059950 MtrunA17_Chr7g0250531 57.647 170 69 3 13 181 4 171 1.34e-58 183
Msa0059950 MtrunA17_Chr8g0335471 49.754 203 97 4 13 214 4 202 1.12e-57 181
Msa0059950 MtrunA17_Chr2g0276931 49.020 204 97 5 13 214 5 203 2.72e-57 180
Msa0059950 MtrunA17_Chr4g0055651 55.357 168 72 3 13 179 4 169 4.55e-57 179
Msa0059950 MtrunA17_Chr1g0182321 44.545 220 109 7 3 214 1 215 9.84e-55 174
Msa0059950 MtrunA17_Chr1g0182301 44.091 220 110 7 3 214 1 215 3.25e-54 172
Msa0059950 MtrunA17_Chr1g0212521 45.714 210 101 7 13 214 11 215 3.09e-53 170
Msa0059950 MtrunA17_Chr4g0030911 45.673 208 103 7 13 214 11 214 1.53e-52 168
Msa0059950 MtrunA17_Chr7g0263071 49.438 178 82 5 13 184 11 186 6.43e-52 166
Msa0059950 MtrunA17_Chr2g0327911 43.137 204 113 3 13 214 11 213 2.10e-51 165
Msa0059950 MtrunA17_Chr6g0458081 49.405 168 82 3 14 180 3 168 9.73e-51 163
Msa0059950 MtrunA17_Chr2g0290891 49.375 160 78 3 14 172 3 160 9.78e-49 158
Msa0059950 MtrunA17_Chr6g0482721 49.708 171 83 3 15 184 10 178 1.77e-48 158
Msa0059950 MtrunA17_Chr8g0349681 42.157 204 111 4 16 214 383 584 7.42e-48 165
Msa0059950 MtrunA17_Chr8g0349681 44.134 179 91 4 1 172 153 329 2.68e-42 150
Msa0059950 MtrunA17_Chr8g0349681 44.099 161 87 3 16 175 12 170 1.18e-40 145
Msa0059950 MtrunA17_Chr2g0325091 44.976 209 106 6 13 214 7 213 1.27e-47 155
Msa0059950 MtrunA17_Chr8g0349711 47.468 158 80 3 16 172 5 160 1.12e-46 153
Msa0059950 MtrunA17_Chr2g0283131 40.092 217 106 6 13 214 9 216 1.36e-46 153
Msa0059950 MtrunA17_Chr4g0046261 42.932 191 95 4 13 191 9 197 1.74e-46 153
Msa0059950 MtrunA17_Chr2g0319951 48.214 168 84 3 13 179 9 174 3.66e-46 152
Msa0059950 MtrunA17_Chr8g0346421 46.429 168 87 3 13 179 22 187 5.93e-46 152
Msa0059950 MtrunA17_Chr2g0276941 40.000 210 115 5 13 213 11 218 1.27e-45 150
Msa0059950 MtrunA17_Chr4g0040781 42.932 191 95 4 13 191 9 197 1.63e-45 150
Msa0059950 MtrunA17_Chr7g0275991 42.051 195 110 3 13 206 16 208 2.63e-45 150
Msa0059950 MtrunA17_Chr3g0112681 42.077 183 100 4 13 191 8 188 2.76e-45 149
Msa0059950 MtrunA17_Chr2g0315551 45.833 168 88 3 13 179 9 174 4.67e-45 149
Msa0059950 MtrunA17_Chr8g0381841 38.679 212 116 5 14 213 14 223 5.43e-45 149
Msa0059950 MtrunA17_Chr7g0234201 42.202 218 93 7 16 214 12 215 1.15e-44 148
Msa0059950 MtrunA17_Chr4g0032201 40.761 184 103 4 13 192 8 189 1.24e-44 148
Msa0059950 MtrunA17_Chr3g0123821 37.264 212 120 5 13 213 12 221 2.15e-44 147
Msa0059950 MtrunA17_Chr8g0351861 41.204 216 109 6 15 214 14 227 1.24e-43 146
Msa0059950 MtrunA17_Chr1g0189361 41.026 195 103 4 15 208 12 195 1.60e-43 145
Msa0059950 MtrunA17_Chr8g0392931 47.059 170 87 3 13 181 10 177 2.00e-42 142
Msa0059950 MtrunA17_Chr7g0231131 42.262 168 94 3 13 179 11 176 5.47e-42 141
Msa0059950 MtrunA17_Chr1g0174281 37.037 216 119 7 13 214 11 223 7.49e-41 138
Msa0059950 MtrunA17_Chr7g0214241 42.353 170 95 3 16 184 14 181 1.44e-38 132
Msa0059950 MtrunA17_Chr5g0433561 37.056 197 115 6 19 214 12 200 3.49e-36 125
Msa0059950 MtrunA17_Chr1g0199331 40.237 169 96 5 19 185 33 198 2.83e-35 123
Msa0059950 MtrunA17_Chr3g0109721 35.533 197 118 6 19 214 12 200 4.67e-35 123
Msa0059950 MtrunA17_Chr8g0390561 38.272 162 97 3 19 179 11 170 6.72e-34 120
Msa0059950 MtrunA17_Chr4g0055081 38.994 159 94 3 18 175 10 166 5.37e-33 118
Msa0059950 MtrunA17_Chr8g0382221 37.736 159 96 3 18 175 10 166 2.97e-32 116
Msa0059950 MtrunA17_Chr5g0400131 38.365 159 95 3 18 175 10 166 3.84e-32 115
Msa0059950 MtrunA17_Chr3g0123971 37.278 169 103 3 18 185 10 176 4.21e-32 115
Msa0059950 MtrunA17_Chr4g0034871 36.842 171 100 5 17 180 8 177 6.05e-29 107
Msa0059950 MtrunA17_Chr8g0383261 39.444 180 94 7 1 172 1 173 7.80e-29 107
Msa0059950 MtrunA17_Chr2g0331691 34.653 202 106 6 17 211 10 192 7.00e-28 104
Msa0059950 MtrunA17_Chr4g0004661 33.663 202 108 6 17 211 64 246 7.95e-27 103
Msa0059950 MtrunA17_Chr1g0201121 37.423 163 94 5 17 172 8 169 1.54e-26 101
Msa0059950 MtrunA17_Chr6g0466301 38.650 163 92 6 17 172 8 169 2.11e-26 100
Msa0059950 MtrunA17_Chr6g0465491 38.650 163 92 6 17 172 8 169 2.11e-26 100
Msa0059950 MtrunA17_Chr3g0138121 36.813 182 106 7 7 185 2 177 1.57e-25 99.0
Msa0059950 MtrunA17_Chr3g0138101 36.813 182 106 7 7 185 2 177 1.79e-25 99.0
Msa0059950 MtrunA17_Chr3g0138111 36.813 182 106 7 7 185 2 177 2.03e-25 99.0
Msa0059950 MtrunA17_Chr2g0325101 31.250 176 106 5 17 180 6 178 1.79e-23 93.2
Msa0059950 MtrunA17_Chr3g0117931 29.064 203 126 6 8 198 2 198 5.02e-23 92.4
Msa0059950 MtrunA17_Chr6g0486041 30.726 179 109 5 17 183 6 181 5.94e-23 92.0
Msa0059950 MtrunA17_Chr4g0037021 29.609 179 111 5 17 183 6 181 2.74e-22 90.1
Msa0059950 MtrunA17_Chr8g0371501 31.250 176 106 5 17 180 6 178 3.19e-21 87.0
Msa0059950 MtrunA17_Chr5g0405791 30.682 176 107 5 17 180 6 178 7.95e-21 86.3
Msa0059950 MtrunA17_Chr4g0046211 32.258 124 80 3 17 139 6 126 1.97e-17 75.9
Msa0059950 MtrunA17_Chr6g0466431 36.000 100 53 3 17 115 8 97 2.13e-14 66.6
Msa0059950 MtrunA17_Chr6g0465611 36.000 100 53 3 17 115 8 97 2.13e-14 66.6
Msa0059950 MtrunA17_Chr6g0466161 36.641 131 76 5 48 172 22 151 3.93e-14 68.6
Msa0059950 MtrunA17_Chr6g0465471 36.641 131 76 5 48 172 22 151 4.31e-14 68.2
Msa0059950 MtrunA17_Chr5g0434561 31.034 174 97 9 14 176 16 177 1.75e-12 63.5
Msa0059950 MtrunA17_Chr8g0383221 30.675 163 83 6 19 153 23 183 3.62e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0059950 AT1G43890.3 85.354 198 29 0 7 204 3 200 4.17e-126 355
Msa0059950 AT1G43890.1 85.354 198 29 0 7 204 3 200 4.17e-126 355
Msa0059950 AT1G43890.2 85.354 198 29 0 7 204 3 200 4.17e-126 355
Msa0059950 AT5G03530.2 66.827 208 69 0 7 214 3 210 1.93e-109 313
Msa0059950 AT5G03530.1 66.827 208 69 0 7 214 3 210 1.93e-109 313
Msa0059950 AT3G09910.3 63.462 208 70 2 7 214 3 204 2.66e-89 261
Msa0059950 AT3G09910.1 63.462 208 70 2 7 214 3 204 2.66e-89 261
Msa0059950 AT3G09910.2 63.462 208 70 2 7 214 3 204 2.66e-89 261
Msa0059950 AT3G09910.5 64.865 185 62 1 7 188 3 187 4.76e-81 240
Msa0059950 AT3G09910.4 64.865 185 62 1 7 188 3 187 4.76e-81 240
Msa0059950 AT1G02130.1 48.768 203 99 4 13 214 4 202 5.44e-57 179
Msa0059950 AT5G47200.1 48.276 203 99 5 13 214 4 201 2.67e-56 177
Msa0059950 AT4G17530.1 48.276 203 99 5 13 214 4 201 4.45e-56 177
Msa0059950 AT4G35860.1 48.925 186 88 4 14 194 3 186 5.64e-54 172
Msa0059950 AT3G53610.2 46.860 207 100 7 13 213 11 213 1.48e-53 171
Msa0059950 AT3G53610.3 46.860 207 100 7 13 213 11 213 1.48e-53 171
Msa0059950 AT3G53610.1 46.860 207 100 7 13 213 11 213 1.48e-53 171
Msa0059950 AT5G59840.1 46.377 207 101 7 13 213 11 213 3.10e-53 170
Msa0059950 AT3G09900.1 47.170 212 100 7 12 215 10 217 1.79e-52 168
Msa0059950 AT3G11730.1 45.320 203 108 3 13 214 4 204 2.06e-52 168
Msa0059950 AT5G03520.1 45.161 217 107 6 6 215 4 215 2.17e-52 168
Msa0059950 AT3G46060.2 45.894 207 102 7 13 213 11 213 5.82e-52 167
Msa0059950 AT3G46060.3 45.894 207 102 7 13 213 11 213 5.82e-52 167
Msa0059950 AT3G46060.1 45.894 207 102 7 13 213 11 213 5.82e-52 167
Msa0059950 AT4G17160.1 42.439 205 112 4 14 214 3 205 1.49e-50 163
Msa0059950 AT4G39990.1 42.105 209 109 5 15 213 15 221 5.29e-50 162
Msa0059950 AT4G17170.1 49.375 160 78 3 14 172 3 160 2.74e-49 160
Msa0059950 AT2G33870.1 41.706 211 112 5 13 214 9 217 1.37e-47 156
Msa0059950 AT5G60860.1 43.455 191 94 4 13 191 9 197 3.05e-46 152
Msa0059950 AT4G18430.1 41.429 210 113 4 13 214 9 216 3.95e-46 152
Msa0059950 AT1G18200.1 48.214 168 84 3 13 179 9 174 4.24e-46 152
Msa0059950 AT1G09630.1 40.000 210 116 4 13 214 8 215 4.45e-46 152
Msa0059950 AT1G73640.1 48.214 168 84 3 13 179 9 174 5.24e-46 152
Msa0059950 AT1G28550.1 47.024 168 86 3 13 179 9 174 5.68e-46 152
Msa0059950 AT1G16920.1 47.619 168 85 3 13 179 9 174 7.11e-46 151
Msa0059950 AT5G45750.1 47.024 168 86 3 13 179 9 174 8.45e-46 151
Msa0059950 AT3G07410.1 41.627 209 111 4 15 214 10 216 1.22e-45 151
Msa0059950 AT1G06400.1 47.619 168 85 3 13 179 9 174 1.92e-45 150
Msa0059950 AT3G15060.1 45.087 173 92 3 13 184 9 179 9.20e-45 149
Msa0059950 AT4G18800.1 41.546 207 114 4 13 214 9 213 1.11e-44 148
Msa0059950 AT2G43130.1 40.777 206 114 5 15 214 10 213 1.58e-44 148
Msa0059950 AT1G05810.1 45.029 171 91 3 15 184 10 178 1.93e-44 148
Msa0059950 AT5G47520.1 44.444 171 92 3 15 184 12 180 3.94e-44 147
Msa0059950 AT1G05810.2 45.029 171 91 3 15 184 56 224 5.43e-44 148
Msa0059950 AT5G65270.1 41.176 170 97 3 13 181 13 180 6.55e-44 147
Msa0059950 AT1G07410.1 40.217 184 104 4 13 192 8 189 1.25e-43 145
Msa0059950 AT3G46830.1 42.197 173 97 3 13 184 8 178 6.95e-43 144
Msa0059950 AT3G46830.2 42.197 173 97 3 13 184 8 178 6.95e-43 144
Msa0059950 AT3G12160.1 38.208 212 119 6 13 214 11 220 7.82e-43 144
Msa0059950 AT2G31680.1 45.783 166 87 3 15 179 10 173 9.22e-43 144
Msa0059950 AT1G01200.1 44.706 170 90 4 13 180 24 191 1.42e-42 144
Msa0059950 AT5G47960.1 36.620 213 122 6 13 214 11 221 3.53e-42 142
Msa0059950 AT5G59150.1 41.040 173 99 3 13 184 8 178 7.24e-42 141
Msa0059950 AT5G03520.2 42.373 177 91 5 45 215 34 205 1.23e-37 130
Msa0059950 AT4G39890.1 38.251 183 109 3 18 197 10 191 1.26e-37 130
Msa0059950 AT4G35860.2 50.407 123 59 2 58 180 2 122 1.79e-36 126
Msa0059950 AT3G54840.1 39.429 175 99 5 6 175 19 191 2.64e-34 121
Msa0059950 AT4G19640.1 36.041 197 117 6 19 214 12 200 2.69e-34 121
Msa0059950 AT5G45130.1 36.041 197 117 6 19 214 12 200 2.78e-34 121
Msa0059950 AT3G54840.3 40.506 158 90 4 20 175 2 157 9.97e-34 119
Msa0059950 AT5G10260.1 39.623 159 93 3 18 175 10 166 1.85e-33 119
Msa0059950 AT3G54840.2 40.764 157 89 4 19 173 35 189 4.88e-33 118
Msa0059950 AT5G45130.2 35.385 195 117 6 21 214 22 208 1.20e-32 117
Msa0059950 AT2G44610.1 38.994 159 94 3 18 175 10 166 1.49e-32 117
Msa0059950 AT2G22290.1 39.623 159 93 3 18 175 10 166 3.10e-32 116
Msa0059950 AT3G18820.1 39.024 164 92 5 17 173 8 170 3.63e-29 108
Msa0059950 AT5G64990.1 37.821 156 94 3 18 172 8 161 7.66e-29 107
Msa0059950 AT1G52280.1 35.149 202 105 6 17 211 8 190 9.59e-29 107
Msa0059950 AT4G09720.1 39.264 163 91 5 17 172 8 169 2.00e-28 106
Msa0059950 AT1G22740.1 38.037 163 93 5 17 172 8 169 3.56e-28 105
Msa0059950 AT3G16100.1 35.149 202 105 6 17 211 8 190 8.71e-28 104
Msa0059950 AT4G09720.4 38.690 168 90 6 17 172 8 174 9.82e-28 104
Msa0059950 AT1G49300.2 38.037 163 93 5 17 172 8 169 1.33e-27 104
Msa0059950 AT1G49300.1 38.037 163 93 5 17 172 8 169 1.33e-27 104
Msa0059950 AT5G39620.1 37.647 170 98 5 18 180 6 174 2.07e-27 103
Msa0059950 AT2G21880.1 37.805 164 92 6 17 172 9 170 7.83e-27 102
Msa0059950 AT4G09720.3 36.782 174 91 6 17 172 8 180 9.57e-27 102
Msa0059950 AT5G55080.1 39.024 164 92 6 18 179 14 171 2.82e-26 101
Msa0059950 AT5G64990.2 36.196 163 94 4 18 172 8 168 4.02e-26 100
Msa0059950 AT5G59150.2 38.211 123 74 2 57 179 2 122 2.36e-25 97.4
Msa0059950 AT4G19640.2 39.844 128 74 3 19 145 12 137 1.07e-24 95.1
Msa0059950 AT4G19640.3 39.098 133 78 3 19 150 12 142 1.61e-24 95.9
Msa0059950 AT2G44690.1 31.667 180 108 5 17 184 18 194 1.70e-24 96.3
Msa0059950 AT5G20010.1 35.789 190 105 8 7 185 2 185 3.64e-24 95.9
Msa0059950 AT5G55190.1 37.356 174 100 7 9 179 4 171 5.00e-24 95.5
Msa0059950 AT5G20020.1 36.932 176 102 7 7 179 2 171 7.08e-24 95.1
Msa0059950 AT4G35020.1 30.168 179 110 5 17 183 6 181 1.01e-22 91.7
Msa0059950 AT4G35020.2 30.168 179 110 5 17 183 6 181 1.01e-22 91.7
Msa0059950 AT4G35020.3 30.168 179 110 5 17 183 6 181 1.01e-22 91.7
Msa0059950 AT3G51300.1 31.034 174 105 5 19 180 8 178 1.30e-22 91.3
Msa0059950 AT3G48040.1 30.526 190 113 7 8 185 2 184 7.80e-22 89.4
Msa0059950 AT4G28950.1 29.379 177 112 5 17 183 6 179 1.00e-21 89.0
Msa0059950 AT2G17800.2 30.114 176 108 5 17 180 6 178 2.04e-21 88.2
Msa0059950 AT2G17800.1 30.114 176 108 5 17 180 6 178 2.04e-21 88.2
Msa0059950 AT4G09720.6 40.458 131 71 4 48 172 6 135 2.22e-21 87.4
Msa0059950 AT4G09720.5 40.458 131 71 4 48 172 6 135 2.22e-21 87.4
Msa0059950 AT4G09720.2 40.458 131 71 4 48 172 6 135 2.22e-21 87.4
Msa0059950 AT1G20090.1 29.545 176 109 5 17 180 5 177 2.46e-21 87.8
Msa0059950 AT1G75840.1 29.545 176 109 5 17 180 6 178 2.82e-21 87.8
Msa0059950 AT4G35950.1 29.545 176 109 5 17 180 6 178 1.09e-20 86.3
Msa0059950 AT5G62880.1 29.189 185 113 6 8 180 2 180 4.00e-20 85.1
Msa0059950 AT5G45970.1 28.488 172 108 5 17 176 6 174 6.67e-20 84.0
Msa0059950 AT3G48040.2 30.939 181 106 7 8 175 2 176 9.31e-20 83.2
Msa0059950 AT2G21880.4 38.636 132 72 5 48 172 16 145 1.46e-19 82.8
Msa0059950 AT2G21880.3 38.636 132 72 5 48 172 16 145 1.46e-19 82.8
Msa0059950 AT2G21880.2 32.927 164 92 7 17 172 9 162 1.49e-17 77.8
Msa0059950 AT5G62880.2 30.000 170 101 6 8 165 2 165 1.97e-17 77.4
Msa0059950 AT3G51290.2 29.605 152 93 4 40 180 631 779 2.31e-15 74.7
Msa0059950 AT4G35020.4 34.483 116 72 3 19 133 8 120 3.12e-15 70.5
Msa0059950 AT4G35020.5 34.483 116 72 3 19 133 8 120 3.12e-15 70.5
Msa0059950 AT5G46025.1 35.577 104 66 1 78 181 6 108 3.71e-15 69.3
Msa0059950 AT5G37680.1 29.545 176 97 9 14 176 16 177 1.22e-12 64.3
Msa0059950 AT3G49870.2 32.934 167 95 9 18 176 1 158 2.39e-12 63.2
Msa0059950 AT3G49870.1 32.749 171 98 9 14 176 16 177 3.97e-12 62.8
Msa0059950 AT5G67560.1 32.749 171 98 9 14 176 16 177 4.40e-12 62.8
Msa0059950 AT5G64813.1 28.221 163 87 6 19 153 24 184 7.61e-11 60.8
Msa0059950 AT5G64813.3 28.221 163 87 6 19 153 24 184 7.61e-11 60.8
Msa0059950 AT5G64813.2 28.221 163 87 6 19 153 24 184 7.61e-11 60.8
Msa0059950 AT3G22950.1 28.244 131 87 3 18 148 18 141 9.47e-11 59.3
Msa0059950 AT3G22950.2 28.244 131 87 3 18 148 18 141 9.47e-11 59.3

Find 60 sgRNAs with CRISPR-Local

Find 282 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTATGATTGGTGATTC+TGG 0.217855 1_2:-17475560 Msa0059950:CDS
ACTGCAGAAGATCCTGAATT+TGG 0.226153 1_2:-17471609 Msa0059950:three_prime_UTR
TTACAAATCTATCTGATATA+TGG 0.238643 1_2:-17472632 Msa0059950:CDS
AGAAGTTAAAACTTGCTATA+TGG 0.282325 1_2:-17474147 Msa0059950:CDS
GAAAGGTTTCGAACACTTAC+TGG 0.285773 1_2:-17474005 Msa0059950:CDS
GTGGACTTTGCAAGGCAATA+TGG 0.296394 1_2:-17472386 Msa0059950:CDS
CTCCTGGCTGAGGGCTCATC+TGG 0.330734 1_2:-17471947 Msa0059950:CDS
AGGTGCACAAGGAATAATTA+TGG 0.331547 1_2:-17473970 Msa0059950:CDS
TGAAAGGGCTGTTACCAAAA+AGG 0.345224 1_2:-17472414 Msa0059950:CDS
AATGGATGTTTCTTCTTCTT+CGG 0.349573 1_2:-17475618 Msa0059950:CDS
GAAGTTAAAACTTGCTATAT+GGG 0.357838 1_2:-17474146 Msa0059950:CDS
CGCGAAAGAAAGCCTCCTTT+TGG 0.376354 1_2:+17471516 None:intergenic
TTGTTCAAGCTTCTTATGAT+TGG 0.383844 1_2:-17475569 Msa0059950:CDS
GATTGCATCAAGATGCTTGT+TGG 0.386118 1_2:-17472576 Msa0059950:CDS
TACAAATCTATCTGATATAT+GGG 0.407172 1_2:-17472631 Msa0059950:CDS
CAGCAGAAGAAACCAAATTC+AGG 0.409745 1_2:+17471597 None:intergenic
TTCTTCTCGTGCAGCTGGAC+AGG 0.417456 1_2:-17474027 Msa0059950:intron
CTTGTGTTGAATGAATTATA+TGG 0.432819 1_2:+17475702 None:intergenic
GTTAAATATGTTACACTTGG+TGG 0.437372 1_2:-17474172 Msa0059950:CDS
TAATGAAACAGAAAGCAAAA+TGG 0.438205 1_2:+17471548 None:intergenic
TTTGGTTTCTTCTGCTGTTG+TGG 0.440540 1_2:-17471591 Msa0059950:three_prime_UTR
AATCGCGGTTTATTCTGAAA+TGG 0.447740 1_2:-17475636 Msa0059950:exon
TACACCAAGCCTCCTGGCTG+AGG 0.454373 1_2:-17471957 Msa0059950:CDS
ATGATTGGTGATTCTGGTGT+TGG 0.454677 1_2:-17475554 Msa0059950:CDS
TCTGCAGTGCAGAGGGGAAT+GGG 0.455809 1_2:+17471624 None:intergenic
GAAGATCTTTCTCCCACCAT+TGG 0.467139 1_2:-17475491 Msa0059950:intron
TGGACTTTGCAAGGCAATAT+GGG 0.468552 1_2:-17472385 Msa0059950:CDS
GAGCCCTCAGCCAGGAGGCT+TGG 0.469370 1_2:+17471953 None:intergenic
GGTGCACAAGGAATAATTAT+GGG 0.473429 1_2:-17473969 Msa0059950:intron
CTTACTGGTTCATATTATAG+AGG 0.473869 1_2:-17473990 Msa0059950:CDS
TTGCACTGCATTCAGTATAG+AGG 0.489926 1_2:+17472360 None:intergenic
TTCTGCAGTGCAGAGGGGAA+TGG 0.490445 1_2:+17471623 None:intergenic
TTTGGATACACCAAGCCTCC+TGG 0.503438 1_2:-17471963 Msa0059950:CDS
ATTCACATCATACTGAAAAG+TGG 0.524648 1_2:+17471822 None:intergenic
TATGAAATCTGTCTCTACAT+AGG 0.529703 1_2:-17471687 Msa0059950:three_prime_UTR
CACCTAGCAGCTGTTGCGCA+TGG 0.530235 1_2:-17471877 Msa0059950:CDS
TCATTATTATGTGAACCAAA+AGG 0.531815 1_2:-17471531 Msa0059950:three_prime_UTR
AGGATCTTCTGCAGTGCAGA+GGG 0.539344 1_2:+17471617 None:intergenic
CACCAGATGAGCCCTCAGCC+AGG 0.548724 1_2:+17471945 None:intergenic
AGGAAGCGGTGGACTTTGCA+AGG 0.556741 1_2:-17472394 Msa0059950:CDS
AAAGTTAAATATGTTACACT+TGG 0.573517 1_2:-17474175 Msa0059950:CDS
CACCATGCGCAACAGCTGCT+AGG 0.581361 1_2:+17471875 None:intergenic
GGTGATGCATCAGACTCTTG+CGG 0.604993 1_2:+17471896 None:intergenic
CAGGATCTTCTGCAGTGCAG+AGG 0.614315 1_2:+17471616 None:intergenic
ACACCAAGCCTCCTGGCTGA+GGG 0.615958 1_2:-17471956 Msa0059950:CDS
CTCGTGCAGCTGGACAGGAA+AGG 0.620766 1_2:-17474022 Msa0059950:intron
TTTGTGAACAAACCAATGGT+GGG 0.622071 1_2:+17475479 None:intergenic
AGCAAACCACACTGTAGACA+AGG 0.624336 1_2:+17471412 None:intergenic
AAACTTGCTATATGGGACAC+GGG 0.627855 1_2:-17474139 Msa0059950:intron
CACATCATACTGAAAAGTGG+AGG 0.630350 1_2:+17471825 None:intergenic
TTATTATGTGAACCAAAAGG+AGG 0.632285 1_2:-17471528 Msa0059950:three_prime_UTR
AAAACTTGCTATATGGGACA+CGG 0.637080 1_2:-17474140 Msa0059950:CDS
ACATCATACTGAAAAGTGGA+GGG 0.649542 1_2:+17471826 None:intergenic
TCATATTATAGAGGTGCACA+AGG 0.659834 1_2:-17473981 Msa0059950:CDS
TGTTGGAAACAAAGTTGACA+AGG 0.666842 1_2:-17472559 Msa0059950:intron
CAGATGAGCCCTCAGCCAGG+AGG 0.684281 1_2:+17471948 None:intergenic
TCGAAGCTTAAACTGAATCG+CGG 0.689908 1_2:-17475651 Msa0059950:five_prime_UTR
AAGATGCCTTGTCTACAGTG+TGG 0.720385 1_2:-17471418 None:intergenic
GAACAAGTAATCGAATTCAG+CGG 0.723999 1_2:+17475586 None:intergenic
GGATCTTCTGCAGTGCAGAG+GGG 0.729498 1_2:+17471618 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACGATAATAAAATAAAAA+AGG - chr1_2:17475040-17475059 Msa0059950:intron 10.0%
! GGGGGGGGGGGGGGGGGGGG+GGG + chr1_2:17475070-17475089 None:intergenic 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG + chr1_2:17475070-17475089 None:intergenic 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG + chr1_2:17475070-17475089 None:intergenic 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG + chr1_2:17475070-17475089 None:intergenic 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG + chr1_2:17475070-17475089 None:intergenic 100.0%
!! TAAACAAATTTATATTGCAT+TGG - chr1_2:17472798-17472817 Msa0059950:intron 15.0%
!! AAACAAATTTATATTGCATT+GGG - chr1_2:17472799-17472818 Msa0059950:intron 15.0%
!!! CATATCATTATTATTTTAGA+AGG + chr1_2:17473521-17473540 None:intergenic 15.0%
!!! AGTATTTCTTTTTATAAGAT+AGG - chr1_2:17473950-17473969 Msa0059950:intron 15.0%
!!! GTATTTCTTTTTATAAGATA+GGG - chr1_2:17473951-17473970 Msa0059950:intron 15.0%
!!! TTATTATTTTCATGTTTTTG+AGG + chr1_2:17474900-17474919 None:intergenic 15.0%
!!! GATTTTTGTCAATTTATGTT+TGG - chr1_2:17471943-17471962 Msa0059950:CDS 20.0%
!! AATAGTAAACAAGCAAAAAA+AGG + chr1_2:17472622-17472641 None:intergenic 20.0%
!! TACGTCTATATTAACATTAT+AGG + chr1_2:17472780-17472799 None:intergenic 20.0%
!! AACAAATTTATATTGCATTG+GGG - chr1_2:17472800-17472819 Msa0059950:intron 20.0%
!! AAAGTTAAATATGTTACACT+TGG - chr1_2:17472988-17473007 Msa0059950:intron 20.0%
!!! TTGAAATTCTGTTATTTTCT+TGG - chr1_2:17473082-17473101 Msa0059950:intron 20.0%
!!! TGAAATTCTGTTATTTTCTT+GGG - chr1_2:17473083-17473102 Msa0059950:intron 20.0%
!! AAAATTGTACATCATATTCA+TGG + chr1_2:17473277-17473296 None:intergenic 20.0%
!!! TAAAAGCATTCAAACAAATT+AGG + chr1_2:17473302-17473321 None:intergenic 20.0%
!!! AACTATGAAAGTATAATAAC+AGG + chr1_2:17473426-17473445 None:intergenic 20.0%
!!! AGTGTATTACTTTTTTCAAA+AGG - chr1_2:17473600-17473619 Msa0059950:intron 20.0%
!! TCAATAACATTGGTATTATA+GGG + chr1_2:17473763-17473782 None:intergenic 20.0%
!! ATCAATAACATTGGTATTAT+AGG + chr1_2:17473764-17473783 None:intergenic 20.0%
!! TATAATACCAATGTTATTGA+TGG - chr1_2:17473763-17473782 Msa0059950:intron 20.0%
!! CATATATGGATATAAAGATT+GGG - chr1_2:17473991-17474010 Msa0059950:CDS 20.0%
!!! TTATTGTATCTTTTGCTTTT+TGG - chr1_2:17474286-17474305 Msa0059950:intron 20.0%
!! TTACAAATCTATCTGATATA+TGG - chr1_2:17474531-17474550 Msa0059950:intron 20.0%
!! TACAAATCTATCTGATATAT+GGG - chr1_2:17474532-17474551 Msa0059950:intron 20.0%
!!! GACTAACGAAATTAATTTTT+TGG + chr1_2:17475397-17475416 None:intergenic 20.0%
! ATATGGAAAGTTGAAAAAGA+AGG + chr1_2:17471447-17471466 None:intergenic 25.0%
! CTTGTGTTGAATGAATTATA+TGG + chr1_2:17471464-17471483 None:intergenic 25.0%
! TCCAAAATGCATGAATAAAA+GGG + chr1_2:17471733-17471752 None:intergenic 25.0%
! ATCCAAAATGCATGAATAAA+AGG + chr1_2:17471734-17471753 None:intergenic 25.0%
!!! CGTACTATTAACATTGATTT+TGG - chr1_2:17471815-17471834 Msa0059950:three_prime_UTR 25.0%
!!! TAACATTGATTTTGGTTCTA+CGG - chr1_2:17471823-17471842 Msa0059950:three_prime_UTR 25.0%
! CAGAGTCAAAATCAAATTTA+GGG + chr1_2:17472128-17472147 None:intergenic 25.0%
! TCAGAGTCAAAATCAAATTT+AGG + chr1_2:17472129-17472148 None:intergenic 25.0%
!! TTATTAGCTTCTTGAAATCA+CGG + chr1_2:17472290-17472309 None:intergenic 25.0%
! TGATTTCAAGAAGCTAATAA+AGG - chr1_2:17472290-17472309 Msa0059950:intron 25.0%
!!! AGACTTTTTGTACTTTTTCA+CGG - chr1_2:17472392-17472411 Msa0059950:CDS 25.0%
! TGATGTGAAATTCTATCAAA+TGG - chr1_2:17472550-17472569 Msa0059950:intron 25.0%
! TTTAAGAATTGAGTGTAAGA+TGG + chr1_2:17472757-17472776 None:intergenic 25.0%
! TACTTTAACCTATAATTGAG+TGG + chr1_2:17472911-17472930 None:intergenic 25.0%
!!! AGAAGTTAAAACTTGCTATA+TGG - chr1_2:17473016-17473035 Msa0059950:intron 25.0%
!!! GAAGTTAAAACTTGCTATAT+GGG - chr1_2:17473017-17473036 Msa0059950:intron 25.0%
! TTTACCATAAAAACACACAA+GGG + chr1_2:17473385-17473404 None:intergenic 25.0%
!! TTTTACCATAAAAACACACA+AGG + chr1_2:17473386-17473405 None:intergenic 25.0%
!!! GAAATAAAATCGATTAGCTT+AGG + chr1_2:17473460-17473479 None:intergenic 25.0%
!!! TCGATTTTATTTCGAAATTC+AGG - chr1_2:17473467-17473486 Msa0059950:intron 25.0%
! TGTTTCATCCAAAATATTGA+CGG + chr1_2:17473633-17473652 None:intergenic 25.0%
!!! TCAATATTTTGGATGAAACA+TGG - chr1_2:17473633-17473652 Msa0059950:intron 25.0%
!! TGTTACTAAGTTTCTCATTT+TGG - chr1_2:17473704-17473723 Msa0059950:intron 25.0%
!!! TTTTGGCATTTGATAATCAT+TGG - chr1_2:17473721-17473740 Msa0059950:intron 25.0%
! TATACAGTGTTGTCAAATAT+CGG + chr1_2:17473906-17473925 None:intergenic 25.0%
! TACTAAAGTCATTGCATATA+TGG - chr1_2:17473977-17473996 Msa0059950:CDS 25.0%
! GCATATATGGATATAAAGAT+TGG - chr1_2:17473990-17474009 Msa0059950:CDS 25.0%
! TGACTAATAACTAATTCTTC+AGG - chr1_2:17474144-17474163 Msa0059950:CDS 25.0%
! GACTAATAACTAATTCTTCA+GGG - chr1_2:17474145-17474164 Msa0059950:CDS 25.0%
! ATTACATTTAGAAGCAAAAC+AGG + chr1_2:17474367-17474386 None:intergenic 25.0%
! AAACTGACTGATAGATAAAT+GGG + chr1_2:17474444-17474463 None:intergenic 25.0%
! TAAACTGACTGATAGATAAA+TGG + chr1_2:17474445-17474464 None:intergenic 25.0%
! TATAAACTTATCAGTGAATC+TGG - chr1_2:17474468-17474487 Msa0059950:intron 25.0%
! ATAAACTTATCAGTGAATCT+GGG - chr1_2:17474469-17474488 Msa0059950:intron 25.0%
! TGCAAATGCAAATTCATAAA+TGG + chr1_2:17474634-17474653 None:intergenic 25.0%
! TCAAAACTTAACACAAAGTT+TGG + chr1_2:17474670-17474689 None:intergenic 25.0%
! ACTTTCCTAAAATATGAGAT+AGG + chr1_2:17474730-17474749 None:intergenic 25.0%
!!! TCATATTTTAGGAAAGTGAA+AGG - chr1_2:17474733-17474752 Msa0059950:intron 25.0%
!!! CATATTTTAGGAAAGTGAAA+GGG - chr1_2:17474734-17474753 Msa0059950:intron 25.0%
! TTCACAAGAAAAAATTGTCA+AGG + chr1_2:17474970-17474989 None:intergenic 25.0%
! TGATAAGTTTAACTACTTGA+AGG + chr1_2:17475013-17475032 None:intergenic 25.0%
!! AAATCAGTATTTTCAGAAAG+TGG - chr1_2:17475134-17475153 Msa0059950:intron 25.0%
!! TGGAAGTAATGTTTCAATTT+TGG - chr1_2:17475154-17475173 Msa0059950:intron 25.0%
!! GGAAGTAATGTTTCAATTTT+GGG - chr1_2:17475155-17475174 Msa0059950:intron 25.0%
!!! TGGTGATTATGATGTTTTTA+AGG - chr1_2:17475306-17475325 Msa0059950:intron 25.0%
! CATCAATGCATTAAAAAAGT+AGG + chr1_2:17475449-17475468 None:intergenic 25.0%
! TAATGAAACAGAAAGCAAAA+TGG + chr1_2:17475618-17475637 None:intergenic 25.0%
! TCATTATTATGTGAACCAAA+AGG - chr1_2:17475632-17475651 Msa0059950:exon 25.0%
AATGGATGTTTCTTCTTCTT+CGG - chr1_2:17471545-17471564 Msa0059950:three_prime_UTR 30.0%
TTGTTCAAGCTTCTTATGAT+TGG - chr1_2:17471594-17471613 Msa0059950:three_prime_UTR 30.0%
GGGGAAAAACATTGAAATAA+GGG + chr1_2:17471711-17471730 None:intergenic 30.0%
AAAATGCATGAATAAAAGGG+GGG + chr1_2:17471730-17471749 None:intergenic 30.0%
CAAAATGCATGAATAAAAGG+GGG + chr1_2:17471731-17471750 None:intergenic 30.0%
CCAAAATGCATGAATAAAAG+GGG + chr1_2:17471732-17471751 None:intergenic 30.0%
!!! TCTGTGTTTTTTCAATGTAG+CGG - chr1_2:17471760-17471779 Msa0059950:three_prime_UTR 30.0%
AGAGTGACATAAAGTGATTT+AGG - chr1_2:17471896-17471915 Msa0059950:CDS 30.0%
CCATACCAAAATCAAACTTA+CGG + chr1_2:17472041-17472060 None:intergenic 30.0%
!! AAGTTTGATTTTGGTATGGT+CGG - chr1_2:17472042-17472061 Msa0059950:intron 30.0%
CCTCAAATGTGATTAATGTT+TGG + chr1_2:17472187-17472206 None:intergenic 30.0%
CCAAACATTAATCACATTTG+AGG - chr1_2:17472184-17472203 Msa0059950:intron 30.0%
CCATATTCTTCTTGAAATCA+TGG + chr1_2:17472343-17472362 None:intergenic 30.0%
CCATGATTTCAAGAAGAATA+TGG - chr1_2:17472340-17472359 Msa0059950:CDS 30.0%
CAAGATATTGCATGAATGTA+GGG - chr1_2:17472509-17472528 Msa0059950:intron 30.0%
AAGATATTGCATGAATGTAG+GGG - chr1_2:17472510-17472529 Msa0059950:intron 30.0%
ATTCTATCAAATGGCATTAG+TGG - chr1_2:17472559-17472578 Msa0059950:intron 30.0%
AATTTAAGGAATCAGAATCG+TGG - chr1_2:17472719-17472738 Msa0059950:intron 30.0%
AATTTATATTGCATTGGGGA+AGG - chr1_2:17472804-17472823 Msa0059950:intron 30.0%
CATATGGCTTCTTAAATGAA+AGG + chr1_2:17472841-17472860 None:intergenic 30.0%
CACAAATCGTAACATTCATA+TGG + chr1_2:17472857-17472876 None:intergenic 30.0%
TAACCTATAATTGAGTGGTA+GGG + chr1_2:17472906-17472925 None:intergenic 30.0%
TTAACCTATAATTGAGTGGT+AGG + chr1_2:17472907-17472926 None:intergenic 30.0%
GTTAAATATGTTACACTTGG+TGG - chr1_2:17472991-17473010 Msa0059950:intron 30.0%
!!! CATGCTGATTCAGTTTATTT+TGG - chr1_2:17473055-17473074 Msa0059950:intron 30.0%
!! CTTACTGGTTCATATTATAG+AGG - chr1_2:17473173-17473192 Msa0059950:intron 30.0%
!! TTATCCCTTGTGTGTTTTTA+TGG - chr1_2:17473378-17473397 Msa0059950:intron 30.0%
TTTAGAAGGAAATGTACAAG+AGG + chr1_2:17473507-17473526 None:intergenic 30.0%
!! TGATATGTTCATTTTCAAGC+TGG - chr1_2:17473534-17473553 Msa0059950:intron 30.0%
!!! ATTCTTTTTCGAGATTGAAG+TGG - chr1_2:17473566-17473585 Msa0059950:intron 30.0%
! GAAATATGCCGTCAATATTT+TGG - chr1_2:17473622-17473641 Msa0059950:intron 30.0%
CAAATCTTCTGTATTTGCAT+AGG + chr1_2:17473686-17473705 None:intergenic 30.0%
AATACCAATGTTATTGATGG+CGG - chr1_2:17473766-17473785 Msa0059950:intron 30.0%
AATGACAAATTTGTGAAGGA+TGG + chr1_2:17473837-17473856 None:intergenic 30.0%
! AAATTTGTCATTGCGGTTTT+CGG - chr1_2:17473845-17473864 Msa0059950:intron 30.0%
GTACTTAAAACCTCAAATCT+AGG - chr1_2:17474034-17474053 Msa0059950:intron 30.0%
! CAAAGTATTTGATCATGACA+TGG + chr1_2:17474071-17474090 None:intergenic 30.0%
! ACTTTGAAAGCTTAAACTGT+CGG - chr1_2:17474085-17474104 Msa0059950:intron 30.0%
CATATTGTACATACATCTTG+AGG + chr1_2:17474200-17474219 None:intergenic 30.0%
AAAGCTGAATAGTGAATTCA+GGG - chr1_2:17474245-17474264 Msa0059950:intron 30.0%
! GTTTGCCTATCTCATATTTT+AGG - chr1_2:17474722-17474741 Msa0059950:intron 30.0%
CAAGAAAAAATTGTCAAGGA+AGG + chr1_2:17474966-17474985 None:intergenic 30.0%
CAAAATCTGGAAACAACAAA+AGG + chr1_2:17475184-17475203 None:intergenic 30.0%
!!! CTTTTGTTGTTTCCAGATTT+TGG - chr1_2:17475182-17475201 Msa0059950:intron 30.0%
ATTCACATCATACTGAAAAG+TGG + chr1_2:17475344-17475363 None:intergenic 30.0%
TATGAAATCTGTCTCTACAT+AGG - chr1_2:17475476-17475495 Msa0059950:intron 30.0%
TTATTATGTGAACCAAAAGG+AGG - chr1_2:17475635-17475654 Msa0059950:exon 30.0%
! AATCGCGGTTTATTCTGAAA+TGG - chr1_2:17471527-17471546 Msa0059950:three_prime_UTR 35.0%
GAACAAGTAATCGAATTCAG+CGG + chr1_2:17471580-17471599 None:intergenic 35.0%
! CTTCTTATGATTGGTGATTC+TGG - chr1_2:17471603-17471622 Msa0059950:three_prime_UTR 35.0%
TTTGTGAACAAACCAATGGT+GGG + chr1_2:17471687-17471706 None:intergenic 35.0%
! TTTTGTGAACAAACCAATGG+TGG + chr1_2:17471688-17471707 None:intergenic 35.0%
GGGGGAAAAACATTGAAATA+AGG + chr1_2:17471712-17471731 None:intergenic 35.0%
! CCCCTTTTATTCATGCATTT+TGG - chr1_2:17471729-17471748 Msa0059950:three_prime_UTR 35.0%
!! GCTCTAGAATTATTCGTTTG+AGG - chr1_2:17471784-17471803 Msa0059950:three_prime_UTR 35.0%
ATTCAAACATGCACTCAAAG+TGG + chr1_2:17472011-17472030 None:intergenic 35.0%
!! TGAAGCCGTAAGTTTGATTT+TGG - chr1_2:17472033-17472052 Msa0059950:intron 35.0%
!! CCGTAAGTTTGATTTTGGTA+TGG - chr1_2:17472038-17472057 Msa0059950:intron 35.0%
AAAAAGTCTTCGCATCTGTT+TGG + chr1_2:17472381-17472400 None:intergenic 35.0%
GCAAGATATTGCATGAATGT+AGG - chr1_2:17472508-17472527 Msa0059950:intron 35.0%
AGATATTGCATGAATGTAGG+GGG - chr1_2:17472511-17472530 Msa0059950:intron 35.0%
AAAAAGCTTCTCTCTCATAG+TGG - chr1_2:17472685-17472704 Msa0059950:intron 35.0%
AAAAGCTTCTCTCTCATAGT+GGG - chr1_2:17472686-17472705 Msa0059950:intron 35.0%
TGAATGTTACGATTTGTGTG+TGG - chr1_2:17472859-17472878 Msa0059950:intron 35.0%
GACTCTTATGAACATAATGC+AGG - chr1_2:17472955-17472974 Msa0059950:intron 35.0%
!! AAAACTTGCTATATGGGACA+CGG - chr1_2:17473023-17473042 Msa0059950:intron 35.0%
! TCATATTATAGAGGTGCACA+AGG - chr1_2:17473182-17473201 Msa0059950:intron 35.0%
AGGTGCACAAGGAATAATTA+TGG - chr1_2:17473193-17473212 Msa0059950:intron 35.0%
GGTGCACAAGGAATAATTAT+GGG - chr1_2:17473194-17473213 Msa0059950:intron 35.0%
CTACGAAATGGAGAATATCA+AGG + chr1_2:17473233-17473252 None:intergenic 35.0%
ATATTCATGGTATAGCAAGC+AGG + chr1_2:17473264-17473283 None:intergenic 35.0%
ATCTTCTGTATTTGCATAGG+AGG + chr1_2:17473683-17473702 None:intergenic 35.0%
CCTTCACAAATTTGTCATTG+CGG - chr1_2:17473838-17473857 Msa0059950:intron 35.0%
CTTAAAACCTCAAATCTAGG+AGG - chr1_2:17474037-17474056 Msa0059950:intron 35.0%
! CTTTGAAAGCTTAAACTGTC+GGG - chr1_2:17474086-17474105 Msa0059950:intron 35.0%
TAACTAATTCTTCAGGGTCA+AGG - chr1_2:17474151-17474170 Msa0059950:CDS 35.0%
GAAAGCTGAATAGTGAATTC+AGG - chr1_2:17474244-17474263 Msa0059950:intron 35.0%
AAGCTGAATAGTGAATTCAG+GGG - chr1_2:17474246-17474265 Msa0059950:intron 35.0%
TGTTGGAAACAAAGTTGACA+AGG - chr1_2:17474604-17474623 Msa0059950:intron 35.0%
! AAATGTTGAGCAGTGTTTTG+AGG - chr1_2:17474836-17474855 Msa0059950:intron 35.0%
!! TTTTGAGGAGCTTGTAATGA+AGG - chr1_2:17474851-17474870 Msa0059950:intron 35.0%
!! TTCATGTTTTTGAGGACATG+AGG + chr1_2:17474892-17474911 None:intergenic 35.0%
ACATCATACTGAAAAGTGGA+GGG + chr1_2:17475340-17475359 None:intergenic 35.0%
TCGAAGCTTAAACTGAATCG+CGG - chr1_2:17471512-17471531 Msa0059950:three_prime_UTR 40.0%
! ATGATTGGTGATTCTGGTGT+TGG - chr1_2:17471609-17471628 Msa0059950:three_prime_UTR 40.0%
!! GGGTTTTGTGAACAAACCAA+TGG + chr1_2:17471691-17471710 None:intergenic 40.0%
! ATCACATTTGAGGCTAGACT+TGG - chr1_2:17472194-17472213 Msa0059950:intron 40.0%
CTAGACTTGGAACCAAATGT+GGG - chr1_2:17472207-17472226 Msa0059950:intron 40.0%
! GTTCACCTAACGTTAGTTTG+CGG - chr1_2:17472259-17472278 Msa0059950:intron 40.0%
AGGAAGCTTCTGTAGTAATG+CGG - chr1_2:17472310-17472329 Msa0059950:intron 40.0%
GCATCTGTTTGGATATGTGT+TGG + chr1_2:17472370-17472389 None:intergenic 40.0%
!! TAGGAGGTTTGGTTTGGTTT+GGG - chr1_2:17472450-17472469 Msa0059950:intron 40.0%
AAAGGACATGTGAGACAACA+AGG + chr1_2:17472604-17472623 None:intergenic 40.0%
!! TGGGTGTTGGGAGAAATTTA+AGG - chr1_2:17472705-17472724 Msa0059950:intron 40.0%
GGGATGATAAAACAGCATCA+TGG + chr1_2:17472886-17472905 None:intergenic 40.0%
CATCCCTACCACTCAATTAT+AGG - chr1_2:17472900-17472919 Msa0059950:intron 40.0%
!! AAACTTGCTATATGGGACAC+GGG - chr1_2:17473024-17473043 Msa0059950:intron 40.0%
GAAAGGTTTCGAACACTTAC+TGG - chr1_2:17473158-17473177 Msa0059950:intron 40.0%
GAACTGTAAATCACGCAACA+TGG - chr1_2:17473329-17473348 Msa0059950:intron 40.0%
!! CGATTAGCTTAGGCTATACA+TGG + chr1_2:17473450-17473469 None:intergenic 40.0%
CTTTCCGCCATCAATAACAT+TGG + chr1_2:17473773-17473792 None:intergenic 40.0%
GACAAATTTGTGAAGGATGG+CGG + chr1_2:17473834-17473853 None:intergenic 40.0%
CCGCAATGACAAATTTGTGA+AGG + chr1_2:17473841-17473860 None:intergenic 40.0%
ATCATAGCCTCCTAGATTTG+AGG + chr1_2:17474047-17474066 None:intergenic 40.0%
AATAGTGAATTCAGGGGTCA+AGG - chr1_2:17474252-17474271 Msa0059950:intron 40.0%
GATTGCATCAAGATGCTTGT+TGG - chr1_2:17474587-17474606 Msa0059950:intron 40.0%
TGAAAGGGCTGTTACCAAAA+AGG - chr1_2:17474749-17474768 Msa0059950:intron 40.0%
TGGACTTTGCAAGGCAATAT+GGG - chr1_2:17474778-17474797 Msa0059950:intron 40.0%
TTGCACTGCATTCAGTATAG+AGG + chr1_2:17474806-17474825 None:intergenic 40.0%
!! AACTCAAGCTTGCTGAAAGT+TGG - chr1_2:17474923-17474942 Msa0059950:intron 40.0%
GCAACTAACTTGAAGCAAGT+AGG + chr1_2:17475105-17475124 None:intergenic 40.0%
CACATCATACTGAAAAGTGG+AGG + chr1_2:17475341-17475360 None:intergenic 40.0%
ACTGCAGAAGATCCTGAATT+TGG - chr1_2:17475554-17475573 Msa0059950:CDS 40.0%
CAGCAGAAGAAACCAAATTC+AGG + chr1_2:17475569-17475588 None:intergenic 40.0%
! TTTGGTTTCTTCTGCTGTTG+TGG - chr1_2:17475572-17475591 Msa0059950:CDS 40.0%
GAAGATCTTTCTCCCACCAT+TGG - chr1_2:17471672-17471691 Msa0059950:three_prime_UTR 45.0%
!! GATTTTGGTATGGTCGGACT+CGG - chr1_2:17472048-17472067 Msa0059950:intron 45.0%
GCTAGACTTGGAACCAAATG+TGG - chr1_2:17472206-17472225 Msa0059950:intron 45.0%
ACAGACGATTCACCCACATT+TGG + chr1_2:17472222-17472241 None:intergenic 45.0%
ATGTGGGTGAATCGTCTGTT+TGG - chr1_2:17472223-17472242 Msa0059950:intron 45.0%
CGTCTGTTTGGATCTGTGTT+TGG - chr1_2:17472235-17472254 Msa0059950:intron 45.0%
TCGTTCCGCAAACTAACGTT+AGG + chr1_2:17472267-17472286 None:intergenic 45.0%
CTCGAAAGTTCCCATCTCAA+GGG + chr1_2:17472423-17472442 None:intergenic 45.0%
CTTGAGATGGGAACTTTCGA+GGG - chr1_2:17472422-17472441 Msa0059950:CDS 45.0%
! GTTCACTAGGAGGTTTGGTT+TGG - chr1_2:17472444-17472463 Msa0059950:intron 45.0%
!! CTAGGAGGTTTGGTTTGGTT+TGG - chr1_2:17472449-17472468 Msa0059950:intron 45.0%
!! AGGAGGTTTGGTTTGGTTTG+GGG - chr1_2:17472451-17472470 Msa0059950:intron 45.0%
!! TTCTCTCTCATAGTGGGTGT+TGG - chr1_2:17472692-17472711 Msa0059950:intron 45.0%
!! TCTCTCTCATAGTGGGTGTT+GGG - chr1_2:17472693-17472712 Msa0059950:intron 45.0%
! GTATTTTCTTCTCGTGCAGC+TGG - chr1_2:17473131-17473150 Msa0059950:intron 45.0%
CAGGAAAGGTAGCTACGAAA+TGG + chr1_2:17473245-17473264 None:intergenic 45.0%
CATGGTATAGCAAGCAGGAA+AGG + chr1_2:17473259-17473278 None:intergenic 45.0%
CAAAAATCCGACACATCCGA+CGG - chr1_2:17473794-17473813 Msa0059950:intron 45.0%
TTGTCAAATATCGGCCATGG+TGG + chr1_2:17473897-17473916 None:intergenic 45.0%
GTGTTGTCAAATATCGGCCA+TGG + chr1_2:17473900-17473919 None:intergenic 45.0%
AAGCTTAAACTGTCGGGTGA+AGG - chr1_2:17474092-17474111 Msa0059950:intron 45.0%
GGCTGTTACCAAAAAGGAAG+CGG - chr1_2:17474755-17474774 Msa0059950:intron 45.0%
TGTTACCAAAAAGGAAGCGG+TGG - chr1_2:17474758-17474777 Msa0059950:intron 45.0%
! AAAGTCCACCGCTTCCTTTT+TGG + chr1_2:17474766-17474785 None:intergenic 45.0%
GTGGACTTTGCAAGGCAATA+TGG - chr1_2:17474777-17474796 Msa0059950:intron 45.0%
!! GTTTTTGAGGACATGAGGAG+AGG + chr1_2:17474887-17474906 None:intergenic 45.0%
TTGAAGCAAGTAGGTGCTTG+GGG + chr1_2:17475096-17475115 None:intergenic 45.0%
CTTGAAGCAAGTAGGTGCTT+GGG + chr1_2:17475097-17475116 None:intergenic 45.0%
ACTTGAAGCAAGTAGGTGCT+TGG + chr1_2:17475098-17475117 None:intergenic 45.0%
!! GGCTTGGTGTATCCAAAATC+TGG + chr1_2:17475197-17475216 None:intergenic 45.0%
!!! ATAATATTTTAAATAGATTA+TGG + chr1_2:17474336-17474355 None:intergenic 5.0%
TCACGGAAAGACCCTTGAGA+TGG - chr1_2:17472409-17472428 Msa0059950:CDS 50.0%
CACGGAAAGACCCTTGAGAT+GGG - chr1_2:17472410-17472429 Msa0059950:CDS 50.0%
CCTCGAAAGTTCCCATCTCA+AGG + chr1_2:17472424-17472443 None:intergenic 50.0%
CCTTGAGATGGGAACTTTCG+AGG - chr1_2:17472421-17472440 Msa0059950:CDS 50.0%
GGAACTTTCGAGGGTTCACT+AGG - chr1_2:17472431-17472450 Msa0059950:intron 50.0%
ACTTTCGAGGGTTCACTAGG+AGG - chr1_2:17472434-17472453 Msa0059950:intron 50.0%
GAATGTAGGGGGAAAGTGAC+AGG - chr1_2:17472522-17472541 Msa0059950:intron 50.0%
! CGTCGGATGTGTCGGATTTT+TGG + chr1_2:17473796-17473815 None:intergenic 50.0%
!! CATGGTGGCATAGCGGATAT+CGG + chr1_2:17473882-17473901 None:intergenic 50.0%
! TCGGGTGAAGGCACAAAAGA+TGG - chr1_2:17474104-17474123 Msa0059950:intron 50.0%
TGAAGCAAGTAGGTGCTTGG+GGG + chr1_2:17475095-17475114 None:intergenic 50.0%
TTTGGATACACCAAGCCTCC+TGG - chr1_2:17475200-17475219 Msa0059950:intron 50.0%
GGTGATGCATCAGACTCTTG+CGG + chr1_2:17475270-17475289 None:intergenic 50.0%
AGGATCTTCTGCAGTGCAGA+GGG + chr1_2:17475549-17475568 None:intergenic 50.0%
! CGCGAAAGAAAGCCTCCTTT+TGG + chr1_2:17475650-17475669 None:intergenic 50.0%
GGTCGGACTCGGAACTGATT+TGG - chr1_2:17472059-17472078 Msa0059950:intron 55.0%
CGAGGGTTCACTAGGAGGTT+TGG - chr1_2:17472439-17472458 Msa0059950:intron 55.0%
TTCTTCTCGTGCAGCTGGAC+AGG - chr1_2:17473136-17473155 Msa0059950:intron 55.0%
AATCCGACACATCCGACGGA+TGG - chr1_2:17473798-17473817 Msa0059950:intron 55.0%
CGATATCCGCTATGCCACCA+TGG - chr1_2:17473880-17473899 Msa0059950:intron 55.0%
TATCGGCCATGGTGGCATAG+CGG + chr1_2:17473889-17473908 None:intergenic 55.0%
! AGGAAGCGGTGGACTTTGCA+AGG - chr1_2:17474769-17474788 Msa0059950:intron 55.0%
AAGCAAGTAGGTGCTTGGGG+GGG + chr1_2:17475093-17475112 None:intergenic 55.0%
GAAGCAAGTAGGTGCTTGGG+GGG + chr1_2:17475094-17475113 None:intergenic 55.0%
TCTGCAGTGCAGAGGGGAAT+GGG + chr1_2:17475542-17475561 None:intergenic 55.0%
TTCTGCAGTGCAGAGGGGAA+TGG + chr1_2:17475543-17475562 None:intergenic 55.0%
GGATCTTCTGCAGTGCAGAG+GGG + chr1_2:17475548-17475567 None:intergenic 55.0%
CAGGATCTTCTGCAGTGCAG+AGG + chr1_2:17475550-17475569 None:intergenic 55.0%
CTCGTGCAGCTGGACAGGAA+AGG - chr1_2:17473141-17473160 Msa0059950:intron 60.0%
GCACCATCCGTCGGATGTGT+CGG + chr1_2:17473804-17473823 None:intergenic 60.0%
! ACATCCGACGGATGGTGCTG+TGG - chr1_2:17473806-17473825 Msa0059950:intron 60.0%
!! GGCATAGCGGATATCGGTGG+CGG + chr1_2:17473876-17473895 None:intergenic 60.0%
!! GGTGGCATAGCGGATATCGG+TGG + chr1_2:17473879-17473898 None:intergenic 60.0%
AGCAAGTAGGTGCTTGGGGG+GGG + chr1_2:17475092-17475111 None:intergenic 60.0%
TACACCAAGCCTCCTGGCTG+AGG - chr1_2:17475206-17475225 Msa0059950:intron 60.0%
ACACCAAGCCTCCTGGCTGA+GGG - chr1_2:17475207-17475226 Msa0059950:intron 60.0%
CACCTAGCAGCTGTTGCGCA+TGG - chr1_2:17475286-17475305 Msa0059950:intron 60.0%
CACCATGCGCAACAGCTGCT+AGG + chr1_2:17475291-17475310 None:intergenic 60.0%
GGCACCACAGCACCATCCGT+CGG + chr1_2:17473813-17473832 None:intergenic 65.0%
AAGTAGGTGCTTGGGGGGGG+GGG + chr1_2:17475089-17475108 None:intergenic 65.0%
CAAGTAGGTGCTTGGGGGGG+GGG + chr1_2:17475090-17475109 None:intergenic 65.0%
GCAAGTAGGTGCTTGGGGGG+GGG + chr1_2:17475091-17475110 None:intergenic 65.0%
CAGATGAGCCCTCAGCCAGG+AGG + chr1_2:17475218-17475237 None:intergenic 65.0%
CTCCTGGCTGAGGGCTCATC+TGG - chr1_2:17475216-17475235 Msa0059950:intron 65.0%
CACCAGATGAGCCCTCAGCC+AGG + chr1_2:17475221-17475240 None:intergenic 65.0%
AGTAGGTGCTTGGGGGGGGG+GGG + chr1_2:17475088-17475107 None:intergenic 70.0%
GAGCCCTCAGCCAGGAGGCT+TGG + chr1_2:17475213-17475232 None:intergenic 70.0%
TAGGTGCTTGGGGGGGGGGG+GGG + chr1_2:17475086-17475105 None:intergenic 75.0%
GTAGGTGCTTGGGGGGGGGG+GGG + chr1_2:17475087-17475106 None:intergenic 75.0%
! AGGTGCTTGGGGGGGGGGGG+GGG + chr1_2:17475085-17475104 None:intergenic 80.0%
! TGCTTGGGGGGGGGGGGGGG+GGG + chr1_2:17475082-17475101 None:intergenic 85.0%
! GTGCTTGGGGGGGGGGGGGG+GGG + chr1_2:17475083-17475102 None:intergenic 85.0%
! GGTGCTTGGGGGGGGGGGGG+GGG + chr1_2:17475084-17475103 None:intergenic 85.0%
! TTGGGGGGGGGGGGGGGGGG+GGG + chr1_2:17475079-17475098 None:intergenic 90.0%
! CTTGGGGGGGGGGGGGGGGG+GGG + chr1_2:17475080-17475099 None:intergenic 90.0%
! GCTTGGGGGGGGGGGGGGGG+GGG + chr1_2:17475081-17475100 None:intergenic 90.0%
! TGGGGGGGGGGGGGGGGGGG+GGG + chr1_2:17475078-17475097 None:intergenic 95.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 17471421 17475764 17471421 ID=Msa0059950;Name=Msa0059950
chr1_2 mRNA 17471421 17475764 17471421 ID=Msa0059950-mRNA-1;Parent=Msa0059950;Name=Msa0059950-mRNA-1;_AED=0.05;_eAED=0.05;_QI=125|1|1|1|1|1|6|453|215
chr1_2 exon 17471421 17471987 17471421 ID=Msa0059950-mRNA-1:exon:6742;Parent=Msa0059950-mRNA-1
chr1_2 exon 17472313 17472441 17472313 ID=Msa0059950-mRNA-1:exon:6741;Parent=Msa0059950-mRNA-1
chr1_2 exon 17472560 17472681 17472560 ID=Msa0059950-mRNA-1:exon:6740;Parent=Msa0059950-mRNA-1
chr1_2 exon 17473970 17474035 17473970 ID=Msa0059950-mRNA-1:exon:6739;Parent=Msa0059950-mRNA-1
chr1_2 exon 17474140 17474208 17474140 ID=Msa0059950-mRNA-1:exon:6738;Parent=Msa0059950-mRNA-1
chr1_2 exon 17475492 17475764 17475492 ID=Msa0059950-mRNA-1:exon:6737;Parent=Msa0059950-mRNA-1
chr1_2 five_prime_UTR 17475640 17475764 17475640 ID=Msa0059950-mRNA-1:five_prime_utr;Parent=Msa0059950-mRNA-1
chr1_2 CDS 17475492 17475639 17475492 ID=Msa0059950-mRNA-1:cds;Parent=Msa0059950-mRNA-1
chr1_2 CDS 17474140 17474208 17474140 ID=Msa0059950-mRNA-1:cds;Parent=Msa0059950-mRNA-1
chr1_2 CDS 17473970 17474035 17473970 ID=Msa0059950-mRNA-1:cds;Parent=Msa0059950-mRNA-1
chr1_2 CDS 17472560 17472681 17472560 ID=Msa0059950-mRNA-1:cds;Parent=Msa0059950-mRNA-1
chr1_2 CDS 17472313 17472441 17472313 ID=Msa0059950-mRNA-1:cds;Parent=Msa0059950-mRNA-1
chr1_2 CDS 17471874 17471987 17471874 ID=Msa0059950-mRNA-1:cds;Parent=Msa0059950-mRNA-1
chr1_2 three_prime_UTR 17471421 17471873 17471421 ID=Msa0059950-mRNA-1:three_prime_utr;Parent=Msa0059950-mRNA-1
Gene Sequence

>Msa0059950

ATGGATGTTTCTTCTTCTTCGGCGAGTAACGCCGCTGAATTCGATTACTTGTTCAAGCTTCTTATGATTGGTGATTCTGGTGTTGGCAAAAGCACGTTGCTTCTTCGATTCACTTCTGATAACTTCGAAGATCTTTCTCCCACCATTGGTGTAGACTTCAAAGTTAAATATGTTACACTTGGTGGAAAGAAGTTAAAACTTGCTATATGGGACACGGCTGGACAGGAAAGGTTTCGAACACTTACTGGTTCATATTATAGAGGTGCACAAGGAATAATTATGGTATACGATGTAACGCGACGTGAAACATTTACAAATCTATCTGATATATGGGCTAAAGAAATTGACTTATACTCAACAAATCAAGATTGCATCAAGATGCTTGTTGGAAACAAAGTTGACAAGGAAAGTGAAAGGGCTGTTACCAAAAAGGAAGCGGTGGACTTTGCAAGGCAATATGGGTGCCTCTATACTGAATGCAGTGCAAAAACTCGAGTAAATGTTGAGCAGTGTTTTGAGGAGCTTGTAATGAAGATTTTGGATACACCAAGCCTCCTGGCTGAGGGCTCATCTGGTGTGAAAAAGAACATCTTCAAGCAGAAACCGCAAGAGTCTGATGCATCACCTAGCAGCTGTTGCGCATGGTGA
Protein sequence

>Msa0059950

MDVSSSSASNAAEFDYLFKLLMIGDSGVGKSTLLLRFTSDNFEDLSPTIGVDFKVKYVTLGGKKLKLAIWDTAGQERFRTLTGSYYRGAQGIIMVYDVTRRETFTNLSDIWAKEIDLYSTNQDCIKMLVGNKVDKESERAVTKKEAVDFARQYGCLYTECSAKTRVNVEQCFEELVMKILDTPSLLAEGSSGVKKNIFKQKPQESDASPSSCCAW*