AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0236620


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0236620 MtrunA17_Chr2g0285861 84.416 77 3 2 1 75 1 70 3.76e-38 121
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0236620 AT2G34585.1 76.190 42 10 0 1 42 1 42 9.84e-19 73.2

Find 19 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGTTAGGGGCGGGGTTGAG+TGG 0.375759 2_2:+9560321 None:intergenic
TCGTCGTCGGGATCGTAATC+AGG 0.386527 2_2:+9560363 None:intergenic
TTGACGACCTTGTTCGAGGC+AGG 0.394231 2_2:+9560407 None:intergenic
GCAGTGGTAGCAGTGATTCT+AGG 0.395537 2_2:-9560459 Msa0236620:CDS
GCAGCGGTGGTGGAGGCGAC+GGG 0.399580 2_2:+9560297 None:intergenic
AGCAGCGGTGGTGGAGGCGA+CGG 0.425781 2_2:+9560296 None:intergenic
AGGATTGAGATTGCGATCAA+TGG 0.439079 2_2:+9560383 None:intergenic
ACTGCGTTGTCTTCGTCGTC+GGG 0.450375 2_2:+9560351 None:intergenic
AACTGCGTTGTCTTCGTCGT+CGG 0.458028 2_2:+9560350 None:intergenic
AGTGAATACCACTTATGAAA+CGG 0.507725 2_2:+9560269 None:intergenic
CAGCGGTGGTGGAGGCGACG+GGG 0.518735 2_2:+9560298 None:intergenic
TGGCTTGACGACCTTGTTCG+AGG 0.522648 2_2:+9560403 None:intergenic
GGGATCATGGGAGCAATCAC+CGG 0.545095 2_2:-9560492 None:intergenic
TGACGACCTTGTTCGAGGCA+GGG 0.551454 2_2:+9560408 None:intergenic
TGTAAACCCTGCCTCGAACA+AGG 0.577165 2_2:-9560414 Msa0236620:CDS
TACCACTGCTATCACTGCAC+CGG 0.586478 2_2:+9560473 None:intergenic
AGCGGTGGTGGAGGCGACGG+GGG 0.608456 2_2:+9560299 None:intergenic
TGGTAGCAGTGATTCTAGGT+TGG 0.618694 2_2:-9560455 Msa0236620:CDS
CACCGGTGCAGTGATAGCAG+TGG 0.664884 2_2:-9560475 Msa0236620:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATGAAACGGTTTTAGCAG+CGG + chr2_2:9560489-9560508 None:intergenic 35.0%
AGGATTGAGATTGCGATCAA+TGG + chr2_2:9560387-9560406 None:intergenic 40.0%
TGGTAGCAGTGATTCTAGGT+TGG - chr2_2:9560312-9560331 Msa0236620:CDS 45.0%
TACCACTGCTATCACTGCAC+CGG + chr2_2:9560297-9560316 None:intergenic 50.0%
GCAGTGGTAGCAGTGATTCT+AGG - chr2_2:9560308-9560327 Msa0236620:CDS 50.0%
TGTAAACCCTGCCTCGAACA+AGG - chr2_2:9560353-9560372 Msa0236620:CDS 50.0%
AACTGCGTTGTCTTCGTCGT+CGG + chr2_2:9560420-9560439 None:intergenic 50.0%
!! TGAAACGGTTTTAGCAGCGG+TGG + chr2_2:9560486-9560505 None:intergenic 50.0%
TGACGACCTTGTTCGAGGCA+GGG + chr2_2:9560362-9560381 None:intergenic 55.0%
TTGACGACCTTGTTCGAGGC+AGG + chr2_2:9560363-9560382 None:intergenic 55.0%
TGGCTTGACGACCTTGTTCG+AGG + chr2_2:9560367-9560386 None:intergenic 55.0%
TCGTCGTCGGGATCGTAATC+AGG + chr2_2:9560407-9560426 None:intergenic 55.0%
ACTGCGTTGTCTTCGTCGTC+GGG + chr2_2:9560419-9560438 None:intergenic 55.0%
!! AACGGTTTTAGCAGCGGTGG+TGG + chr2_2:9560483-9560502 None:intergenic 55.0%
CACCGGTGCAGTGATAGCAG+TGG - chr2_2:9560292-9560311 Msa0236620:CDS 60.0%
!! GGTTTTAGCAGCGGTGGTGG+AGG + chr2_2:9560480-9560499 None:intergenic 60.0%
GCGTTAGGGGCGGGGTTGAG+TGG + chr2_2:9560449-9560468 None:intergenic 70.0%
!! TGGAGGCGACGGGGGCGTTA+GGG + chr2_2:9560463-9560482 None:intergenic 70.0%
!! AGCAGCGGTGGTGGAGGCGA+CGG + chr2_2:9560474-9560493 None:intergenic 70.0%
!! GGAGGCGACGGGGGCGTTAG+GGG + chr2_2:9560462-9560481 None:intergenic 75.0%
!! GTGGAGGCGACGGGGGCGTT+AGG + chr2_2:9560464-9560483 None:intergenic 75.0%
!! AGCGGTGGTGGAGGCGACGG+GGG + chr2_2:9560471-9560490 None:intergenic 75.0%
!! CAGCGGTGGTGGAGGCGACG+GGG + chr2_2:9560472-9560491 None:intergenic 75.0%
!! GCAGCGGTGGTGGAGGCGAC+GGG + chr2_2:9560473-9560492 None:intergenic 75.0%
!!! CGACGGGGGCGTTAGGGGCG+GGG + chr2_2:9560457-9560476 None:intergenic 80.0%
!!! GCGACGGGGGCGTTAGGGGC+GGG + chr2_2:9560458-9560477 None:intergenic 80.0%
!!! GGCGACGGGGGCGTTAGGGG+CGG + chr2_2:9560459-9560478 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 9560281 9560508 9560281 ID=Msa0236620;Name=Msa0236620
chr2_2 mRNA 9560281 9560508 9560281 ID=Msa0236620-mRNA-1;Parent=Msa0236620;Name=Msa0236620-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|75
chr2_2 exon 9560281 9560508 9560281 ID=Msa0236620-mRNA-1:exon:4155;Parent=Msa0236620-mRNA-1
chr2_2 CDS 9560281 9560508 9560281 ID=Msa0236620-mRNA-1:cds;Parent=Msa0236620-mRNA-1
Gene Sequence

>Msa0236620

ATGGGAGCAATCACCGGTGCAGTGATAGCAGTGGTAGCAGTGATTCTAGGTTGGATCACAATAGAGATCGCGTGTAAACCCTGCCTCGAACAAGGTCGTCAAGCCATTGATCGCAATCTCAATCCTGATTACGATCCCGACGACGAAGACAACGCAGTTCGTGCTCCACTCAACCCCGCCCCTAACGCCCCCGTCGCCTCCACCACCGCTGCTAAAACCGTTTCATAA

Protein sequence

>Msa0236620

MGAITGAVIAVVAVILGWITIEIACKPCLEQGRQAIDRNLNPDYDPDDEDNAVRAPLNPAPNAPVASTTAAKTVS*