AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1437910


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1437910 MtrunA17_Chr7g0266361 93.939 132 3 1 1 127 1 132 6.73e-85 243
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1437910 AT3G10120.1 28.177 181 66 8 1 126 1 172 1.99e-12 61.2

Find 23 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGTACATCTTGAACTTC+TGG 0.229795 tig0028400:+27189 None:intergenic
CTCACCTTCTTGCGCTCTTT+TGG 0.237188 tig0028400:+27222 None:intergenic
TCTCTTGTTCATGGTGAAAA+TGG 0.241059 tig0028400:-27075 Msa1437910:CDS
TCAGGTATGCTTTGCAATAC+TGG 0.263195 tig0028400:+26997 None:intergenic
AATAAACATGGGAAATTGTT+TGG 0.325749 tig0028400:-27355 None:intergenic
ATCTATTGCTAGATTACTTC+AGG 0.354532 tig0028400:+26979 None:intergenic
TTGCAAGAGATGCTGGATAA+TGG 0.374576 tig0028400:-27123 Msa1437910:CDS
AAAACCTCTTCAGCTTTGAT+AGG 0.399855 tig0028400:+27261 None:intergenic
GTGAAGATCATGAAAACAAA+TGG 0.479078 tig0028400:-27312 Msa1437910:CDS
AAGATGCTATCTCTTGTTCA+TGG 0.483231 tig0028400:-27084 Msa1437910:CDS
AAAAGTGATGATGCCTGTGC+AGG 0.529059 tig0028400:-27027 Msa1437910:CDS
TACAGAAAAGTAGTGCAGTT+AGG 0.532146 tig0028400:-27173 Msa1437910:CDS
TACTGGTTTCCACCCTGCAC+AGG 0.563255 tig0028400:+27014 None:intergenic
TGTTCATGGTGAAAATGGAA+TGG 0.589839 tig0028400:-27070 Msa1437910:CDS
AACACCTATCAAAGCTGAAG+AGG 0.600750 tig0028400:-27265 Msa1437910:CDS
CAAGAGATGCTGGATAATGG+AGG 0.604501 tig0028400:-27120 Msa1437910:CDS
CAAAAGAGCGCAAGAAGGTG+AGG 0.614183 tig0028400:-27221 Msa1437910:CDS
CATGGTGAAAATGGAATGGA+TGG 0.619170 tig0028400:-27066 Msa1437910:CDS
ATCACCAAAAGAGCGCAAGA+AGG 0.623997 tig0028400:-27226 Msa1437910:CDS
AAGAGATGCTGGATAATGGA+GGG 0.645439 tig0028400:-27119 Msa1437910:CDS
AAAGTGATGATGCCTGTGCA+GGG 0.662931 tig0028400:-27026 Msa1437910:CDS
GGTGCTGCAACAAATTAATG+TGG 0.691279 tig0028400:-27334 Msa1437910:CDS
GTGATGATGCCTGTGCAGGG+TGG 0.757120 tig0028400:-27023 Msa1437910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GTGAAGATCATGAAAACAAA+TGG - tig0028400:27023-27042 Msa1437910:CDS 30.0%
! AAAACCTCTTCAGCTTTGAT+AGG + tig0028400:27077-27096 None:intergenic 35.0%
TTCTGTACATCTTGAACTTC+TGG + tig0028400:27149-27168 None:intergenic 35.0%
TACAGAAAAGTAGTGCAGTT+AGG - tig0028400:27162-27181 Msa1437910:CDS 35.0%
AAGATGCTATCTCTTGTTCA+TGG - tig0028400:27251-27270 Msa1437910:CDS 35.0%
TCTCTTGTTCATGGTGAAAA+TGG - tig0028400:27260-27279 Msa1437910:CDS 35.0%
TGTTCATGGTGAAAATGGAA+TGG - tig0028400:27265-27284 Msa1437910:CDS 35.0%
! GGTGCTGCAACAAATTAATG+TGG - tig0028400:27001-27020 Msa1437910:CDS 40.0%
AACACCTATCAAAGCTGAAG+AGG - tig0028400:27070-27089 Msa1437910:CDS 40.0%
!!! TCTTTTGGTGATGATGGAAG+AGG + tig0028400:27101-27120 None:intergenic 40.0%
GCAAAAATTGCAAGAGATGC+TGG - tig0028400:27205-27224 Msa1437910:CDS 40.0%
TTGCAAGAGATGCTGGATAA+TGG - tig0028400:27212-27231 Msa1437910:CDS 40.0%
!! AAGAGATGCTGGATAATGGA+GGG - tig0028400:27216-27235 Msa1437910:CDS 40.0%
CATGGTGAAAATGGAATGGA+TGG - tig0028400:27269-27288 Msa1437910:CDS 40.0%
TCAGGTATGCTTTGCAATAC+TGG + tig0028400:27341-27360 None:intergenic 40.0%
!!! TTGCGCTCTTTTGGTGATGA+TGG + tig0028400:27107-27126 None:intergenic 45.0%
! ATCACCAAAAGAGCGCAAGA+AGG - tig0028400:27109-27128 Msa1437910:CDS 45.0%
! CAAGAGATGCTGGATAATGG+AGG - tig0028400:27215-27234 Msa1437910:CDS 45.0%
! AAAAGTGATGATGCCTGTGC+AGG - tig0028400:27308-27327 Msa1437910:CDS 45.0%
! AAAGTGATGATGCCTGTGCA+GGG - tig0028400:27309-27328 Msa1437910:CDS 45.0%
! CTCACCTTCTTGCGCTCTTT+TGG + tig0028400:27116-27135 None:intergenic 50.0%
! CAAAAGAGCGCAAGAAGGTG+AGG - tig0028400:27114-27133 Msa1437910:CDS 50.0%
!! TACTGGTTTCCACCCTGCAC+AGG + tig0028400:27324-27343 None:intergenic 55.0%
! GTGATGATGCCTGTGCAGGG+TGG - tig0028400:27312-27331 Msa1437910:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0028400 gene 26987 27370 26987 ID=Msa1437910;Name=Msa1437910
tig0028400 mRNA 26987 27370 26987 ID=Msa1437910-mRNA-1;Parent=Msa1437910;Name=Msa1437910-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|127
tig0028400 exon 26987 27370 26987 ID=Msa1437910-mRNA-1:exon:11029;Parent=Msa1437910-mRNA-1
tig0028400 CDS 26987 27370 26987 ID=Msa1437910-mRNA-1:cds;Parent=Msa1437910-mRNA-1
Gene Sequence

>Msa1437910

ATGGGAAATTGTTTGGTGCTGCAACAAATTAATGTGGTGAAGATCATGAAAACAAATGGAAAAATTCTTGAATACAAAACACAAACACCTATCAAAGCTGAAGAGGTTTTGCCTCTTCCATCATCACCAAAAGAGCGCAAGAAGGTGAGGTTTGCAGATCCAGAAGTTCAAGATGTACAGAAAAGTAGTGCAGTTAGGATTAAGCTTGTTATTAGTAAGCAAAAATTGCAAGAGATGCTGGATAATGGAGGGATTTCAGTTGAAAAGATGCTATCTCTTGTTCATGGTGAAAATGGAATGGATGGTGAAGATTTGTGTAAAAAAAGTGATGATGCCTGTGCAGGGTGGAAACCAGTATTGCAAAGCATACCTGAAGTAATCTAG

Protein sequence

>Msa1437910

MGNCLVLQQINVVKIMKTNGKILEYKTQTPIKAEEVLPLPSSPKERKKVRFADPEVQDVQKSSAVRIKLVISKQKLQEMLDNGGISVEKMLSLVHGENGMDGEDLCKKSDDACAGWKPVLQSIPEVI*