Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1437910 | XP_003625829.2 | 93.939 | 132 | 3 | 1 | 1 | 127 | 1 | 132 | 7.32e-81 | 243 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004840 | Msa1437910 | 0.816908 | 4.339052e-52 | -8.615850e-47 |
| Msa0006040 | Msa1437910 | 0.832094 | 1.150183e-55 | -8.615850e-47 |
| Msa0007440 | Msa1437910 | 0.808404 | 3.149061e-50 | -8.615850e-47 |
| Msa0015090 | Msa1437910 | 0.808598 | 2.862318e-50 | -8.615850e-47 |
| Msa0028660 | Msa1437910 | 0.806510 | 7.941386e-50 | -8.615850e-47 |
| Msa0029850 | Msa1437910 | 0.821876 | 3.197580e-53 | -8.615850e-47 |
| Msa0032470 | Msa1437910 | 0.812274 | 4.603552e-51 | -8.615850e-47 |
| Msa0036140 | Msa1437910 | 0.806907 | 6.549181e-50 | -8.615850e-47 |
| Msa0037880 | Msa1437910 | 0.824773 | 6.730179e-54 | -8.615850e-47 |
| Msa0049750 | Msa1437910 | 0.802576 | 5.253627e-49 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1437910 | MtrunA17_Chr7g0266361 | 93.939 | 132 | 3 | 1 | 1 | 127 | 1 | 132 | 6.73e-85 | 243 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1437910 | AT3G10120.1 | 28.177 | 181 | 66 | 8 | 1 | 126 | 1 | 172 | 1.99e-12 | 61.2 |
Find 23 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCTCTTCAGCTTTGAT+AGG | 0.399855 | tig0028400:+27261 | None:intergenic |
| AAAAGTGATGATGCCTGTGC+AGG | 0.529059 | tig0028400:-27027 | Msa1437910:CDS |
| AAAGTGATGATGCCTGTGCA+GGG | 0.662931 | tig0028400:-27026 | Msa1437910:CDS |
| AACACCTATCAAAGCTGAAG+AGG | 0.600750 | tig0028400:-27265 | Msa1437910:CDS |
| AAGAGATGCTGGATAATGGA+GGG | 0.645439 | tig0028400:-27119 | Msa1437910:CDS |
| AAGATGCTATCTCTTGTTCA+TGG | 0.483231 | tig0028400:-27084 | Msa1437910:CDS |
| AATAAACATGGGAAATTGTT+TGG | 0.325749 | tig0028400:-27355 | None:intergenic |
| ATCACCAAAAGAGCGCAAGA+AGG | 0.623997 | tig0028400:-27226 | Msa1437910:CDS |
| ATCTATTGCTAGATTACTTC+AGG | 0.354532 | tig0028400:+26979 | None:intergenic |
| CAAAAGAGCGCAAGAAGGTG+AGG | 0.614183 | tig0028400:-27221 | Msa1437910:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTGAAGATCATGAAAACAAA+TGG | - | tig0028400:27023-27042 | Msa1437910:CDS | 30.0% | |
| TTCTGTACATCTTGAACTTC+TGG | + | tig0028400:27149-27168 | None:intergenic | 35.0% | |
| TACAGAAAAGTAGTGCAGTT+AGG | - | tig0028400:27162-27181 | Msa1437910:CDS | 35.0% | |
| AAGATGCTATCTCTTGTTCA+TGG | - | tig0028400:27251-27270 | Msa1437910:CDS | 35.0% | |
| TCTCTTGTTCATGGTGAAAA+TGG | - | tig0028400:27260-27279 | Msa1437910:CDS | 35.0% | |
| TGTTCATGGTGAAAATGGAA+TGG | - | tig0028400:27265-27284 | Msa1437910:CDS | 35.0% | |
| AACACCTATCAAAGCTGAAG+AGG | - | tig0028400:27070-27089 | Msa1437910:CDS | 40.0% | |
| GCAAAAATTGCAAGAGATGC+TGG | - | tig0028400:27205-27224 | Msa1437910:CDS | 40.0% | |
| TTGCAAGAGATGCTGGATAA+TGG | - | tig0028400:27212-27231 | Msa1437910:CDS | 40.0% | |
| CATGGTGAAAATGGAATGGA+TGG | - | tig0028400:27269-27288 | Msa1437910:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0028400 | gene | 26987 | 27370 | 26987 | ID=Msa1437910;Name=Msa1437910 |
| tig0028400 | mRNA | 26987 | 27370 | 26987 | ID=Msa1437910-mRNA-1;Parent=Msa1437910;Name=Msa1437910-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|127 |
| tig0028400 | exon | 26987 | 27370 | 26987 | ID=Msa1437910-mRNA-1:exon:11029;Parent=Msa1437910-mRNA-1 |
| tig0028400 | CDS | 26987 | 27370 | 26987 | ID=Msa1437910-mRNA-1:cds;Parent=Msa1437910-mRNA-1 |
| Gene Sequence |
>Msa1437910 ATGGGAAATTGTTTGGTGCTGCAACAAATTAATGTGGTGAAGATCATGAAAACAAATGGAAAAATTCTTGAATACAAAACACAAACACCTATCAAAGCTGAAGAGGTTTTGCCTCTTCCATCATCACCAAAAGAGCGCAAGAAGGTGAGGTTTGCAGATCCAGAAGTTCAAGATGTACAGAAAAGTAGTGCAGTTAGGATTAAGCTTGTTATTAGTAAGCAAAAATTGCAAGAGATGCTGGATAATGGAGGGATTTCAGTTGAAAAGATGCTATCTCTTGTTCATGGTGAAAATGGAATGGATGGTGAAGATTTGTGTAAAAAAAGTGATGATGCCTGTGCAGGGTGGAAACCAGTATTGCAAAGCATACCTGAAGTAATCTAG |
| Protein sequence |
>Msa1437910 MGNCLVLQQINVVKIMKTNGKILEYKTQTPIKAEEVLPLPSSPKERKKVRFADPEVQDVQKSSAVRIKLVISKQKLQEMLDNGGISVEKMLSLVHGENGMDGEDLCKKSDDACAGWKPVLQSIPEVI* |
