AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0095280


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0095280 MtrunA17_Chr8g0355631 100.000 37 0 0 1 37 35 71 2.22e-20 74.7
Msa0095280 MtrunA17_Chr4g0015761 100.000 37 0 0 1 37 35 71 2.56e-20 74.7
Msa0095280 MtrunA17_CPg0492811 100.000 37 0 0 1 37 35 71 2.56e-20 74.7
Msa0095280 MtrunA17_Chr4g0016861 100.000 35 0 0 1 35 1 35 9.96e-19 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0095280 ATCG00600.1 97.222 36 1 0 1 36 1 36 2.23e-18 69.3

Find 14 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGATTGAAGTTTCTCTATT+TGG 0.115333 1_2:-79089777 Msa0095280:CDS
TCATGTCAATTGCCAATTCT+TGG 0.245809 1_2:-79089881 Msa0095280:five_prime_UTR
AATAATCCAGCCAAAGTAAT+AGG 0.315680 1_2:+79089735 None:intergenic
AAACTTCAATCATTTGAGTT+TGG 0.328054 1_2:+79089787 None:intergenic
CTAATTCCTATTACTTTGGC+TGG 0.352028 1_2:-79089741 Msa0095280:CDS
TCTCTATTTGGAATCGTGTT+AGG 0.358426 1_2:-79089765 Msa0095280:CDS
AGGTCTAATTCCTATTACTT+TGG 0.388074 1_2:-79089745 Msa0095280:CDS
ATCATTTGAGTTTGGTTGAA+AGG 0.406278 1_2:+79089795 None:intergenic
GAATCTCAATGACCAAGAAT+TGG 0.414372 1_2:+79089869 None:intergenic
AATTGGCAATTGACATGATA+AGG 0.448909 1_2:+79089886 None:intergenic
TTCTTGGTCATTGAGATTCA+CGG 0.471290 1_2:-79089865 Msa0095280:intron
ATATCGACGTGGTGATCAGT+TGG 0.572503 1_2:-79089694 Msa0095280:CDS
TTTGGCTGGATTATTTGTAA+CGG 0.592623 1_2:-79089727 Msa0095280:CDS
GCATATTTACAATATCGACG+TGG 0.789819 1_2:-79089705 Msa0095280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTTCAATCATTTGAGTT+TGG + chr1_2:79089793-79089812 None:intergenic 25.0%
! ATGATTGAAGTTTCTCTATT+TGG - chr1_2:79089800-79089819 Msa0095280:five_prime_UTR 25.0%
AATTGGCAATTGACATGATA+AGG + chr1_2:79089694-79089713 None:intergenic 30.0%
TCAATACAGATTCCTATTGA+AGG - chr1_2:79089735-79089754 Msa0095280:CDS 30.0%
GGTAATATCTATCCTTCAAT+AGG + chr1_2:79089750-79089769 None:intergenic 30.0%
TGGTTGAAAGGAAAAAAAGT+GGG + chr1_2:79089773-79089792 None:intergenic 30.0%
TTGGTTGAAAGGAAAAAAAG+TGG + chr1_2:79089774-79089793 None:intergenic 30.0%
! ATCATTTGAGTTTGGTTGAA+AGG + chr1_2:79089785-79089804 None:intergenic 30.0%
AGGTCTAATTCCTATTACTT+TGG - chr1_2:79089832-79089851 Msa0095280:intron 30.0%
AATAATCCAGCCAAAGTAAT+AGG + chr1_2:79089845-79089864 None:intergenic 30.0%
TTTGGCTGGATTATTTGTAA+CGG - chr1_2:79089850-79089869 Msa0095280:intron 30.0%
TCATGTCAATTGCCAATTCT+TGG - chr1_2:79089696-79089715 Msa0095280:CDS 35.0%
GAATCTCAATGACCAAGAAT+TGG + chr1_2:79089711-79089730 None:intergenic 35.0%
TTCTTGGTCATTGAGATTCA+CGG - chr1_2:79089712-79089731 Msa0095280:CDS 35.0%
GTTGAAAGGAAAAAAAGTGG+GGG + chr1_2:79089771-79089790 None:intergenic 35.0%
GGTTGAAAGGAAAAAAAGTG+GGG + chr1_2:79089772-79089791 None:intergenic 35.0%
! TCTCTATTTGGAATCGTGTT+AGG - chr1_2:79089812-79089831 Msa0095280:five_prime_UTR 35.0%
CTAATTCCTATTACTTTGGC+TGG - chr1_2:79089836-79089855 Msa0095280:intron 35.0%
GCATATTTACAATATCGACG+TGG - chr1_2:79089872-79089891 Msa0095280:five_prime_UTR 35.0%
ATATCGACGTGGTGATCAGT+TGG - chr1_2:79089883-79089902 Msa0095280:five_prime_UTR 45.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 79089689 79089910 79089689 ID=Msa0095280;Name=Msa0095280
chr1_2 mRNA 79089689 79089910 79089689 ID=Msa0095280-mRNA-1;Parent=Msa0095280;Name=Msa0095280-mRNA-1;_AED=0.41;_eAED=0.66;_QI=83|0|0|1|0|0|2|0|37
chr1_2 exon 79089866 79089910 79089866 ID=Msa0095280-mRNA-1:exon:28106;Parent=Msa0095280-mRNA-1
chr1_2 exon 79089689 79089837 79089689 ID=Msa0095280-mRNA-1:exon:28105;Parent=Msa0095280-mRNA-1
chr1_2 five_prime_UTR 79089866 79089910 79089866 ID=Msa0095280-mRNA-1:five_prime_utr;Parent=Msa0095280-mRNA-1
chr1_2 five_prime_UTR 79089800 79089837 79089800 ID=Msa0095280-mRNA-1:five_prime_utr;Parent=Msa0095280-mRNA-1
chr1_2 CDS 79089689 79089799 79089689 ID=Msa0095280-mRNA-1:cds;Parent=Msa0095280-mRNA-1
Gene Sequence

>Msa0095280

ATGATTGAAGTTTCTCTATTTGGAATCGTGTTAGGTCTAATTCCTATTACTTTGGCTGGATTATTTGTAACGGCATATTTACAATATCGACGTGGTGATCAGTTGGATATT

Protein sequence

>Msa0095280

MIEVSLFGIVLGLIPITLAGLFVTAYLQYRRGDQLDI