AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72174

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72174.t1 MTR_8g097290 91.905 803 27 3 1 773 70 864 0.0 1458
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72174.t1 AT1G12800 43.766 786 323 18 1 773 64 743 0.0 546

Find 153 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTTTAGCTCCCACATTTC+AGG 0.170273 4.2:+6840670 MS.gene72174:CDS
GATTTCAATCCTTGGGGTTT+GGG 0.193475 4.2:-6833895 None:intergenic
TGTTGATGTGTTAGATATTC+AGG 0.207545 4.2:+6835156 MS.gene72174:CDS
TTCCAAAGTAAGTTATCTTT+TGG 0.224723 4.2:-6836135 None:intergenic
GTTAGTTCTTCCTTCTCTTT+TGG 0.246221 4.2:-6835948 None:intergenic
GACCATTTACAGGTTTCTTT+TGG 0.271510 4.2:+6835456 MS.gene72174:intron
GGATTTCAATCCTTGGGGTT+TGG 0.279989 4.2:-6833896 None:intergenic
TTTGAGGTTTGTTTCTCATA+TGG 0.280618 4.2:-6835064 None:intergenic
AAGTTCTTATCATCATTTAT+TGG 0.288189 4.2:+6835741 MS.gene72174:CDS
AGATCTTCCAATTTCATATC+TGG 0.288740 4.2:-6835690 None:intergenic
GTTATCACTATCATCAGATT+TGG 0.302013 4.2:-6833917 None:intergenic
CTCAAATTCGGTCGTTTACT+TGG 0.305905 4.2:+6833483 MS.gene72174:CDS
TCAATCTTAAAATAATTAGA+TGG 0.307787 4.2:-6836434 None:intergenic
AATTTCACCAGATATGAAAT+TGG 0.313854 4.2:+6835683 MS.gene72174:CDS
AGATTTGGATTTCAATCCTT+GGG 0.334723 4.2:-6833902 None:intergenic
TGCTGATTCACCTTCACATA+TGG 0.336855 4.2:+6835635 MS.gene72174:CDS
TCTTTGTCACTTGCACTTTG+TGG 0.341871 4.2:-6834603 None:intergenic
AGATTAGCACCTACATCAAT+TGG 0.345883 4.2:-6834747 None:intergenic
TCACTGTTTCGACGACTTCC+AGG 0.345895 4.2:-6835118 None:intergenic
AAAGTAGAAGAAGAGAAGTT+TGG 0.349842 4.2:+6834705 MS.gene72174:CDS
GGTTTCCGCAGGGCTTCATT+AGG 0.353507 4.2:-6833964 None:intergenic
CTTGTACTGATTCTCAAATA+TGG 0.356783 4.2:-6841208 None:intergenic
ACCTTGGCAAGGGTGAGCTT+AGG 0.357739 4.2:-6833513 None:intergenic
AGTCTCAAATTTGACCTTGT+TGG 0.365483 4.2:+6834575 MS.gene72174:CDS
GCCCAAGAGCAGAGTTTATC+TGG 0.369434 4.2:-6837633 None:intergenic
TCTTTGCTACTTGCTCTTTG+TGG 0.371891 4.2:-6834522 None:intergenic
CAGATTTGGATTTCAATCCT+TGG 0.375039 4.2:-6833903 None:intergenic
CAATACAAGGAAAGCCAAAA+AGG 0.377381 4.2:+6834952 MS.gene72174:CDS
GTCTCCAACTACAGACTCTA+AGG 0.385731 4.2:-6837795 None:intergenic
CAAGGTCTTTCAACTTCAAA+TGG 0.390599 4.2:-6833856 None:intergenic
AGGAAGCCTGGATCGGTAAT+TGG 0.394951 4.2:+6834168 MS.gene72174:CDS
CACCAGATAAACTCTGCTCT+TGG 0.395984 4.2:+6837631 MS.gene72174:CDS
TCCACTTTGACAGAAGATTT+CGG 0.401680 4.2:-6835503 None:intergenic
ATCTCCCATCAAAGGGTTCA+TGG 0.406851 4.2:-6837817 None:intergenic
GGAGACCATGAACCCTTTGA+TGG 0.413785 4.2:+6837812 MS.gene72174:CDS
ACTTTGTCGCCTGCTCTTTG+TGG 0.414821 4.2:-6834687 None:intergenic
AGCAAAGAAGAAGAGCAGTT+TGG 0.415432 4.2:+6834537 MS.gene72174:CDS
CTTATCATCATTTATTGGAC+AGG 0.419098 4.2:+6835746 MS.gene72174:CDS
TGAACCCTTTGATGGGAGAT+TGG 0.423539 4.2:+6837820 MS.gene72174:CDS
GGCTTTCTCAAAATAACATT+AGG 0.429930 4.2:-6834012 None:intergenic
TTCTTACCAATTACCGATCC+AGG 0.430760 4.2:-6834174 None:intergenic
AGTCGCAAAATTAACCTTGT+TGG 0.432749 4.2:+6834494 MS.gene72174:CDS
TAACATTTCCGGGTTTCCGC+AGG 0.436095 4.2:-6833975 None:intergenic
GCAGAAGATTTGATTAAGAC+TGG 0.436189 4.2:+6835313 MS.gene72174:CDS
ATATCAAGATAAGATGCATC+AGG 0.438714 4.2:-6833778 None:intergenic
CCTTGGCAAGGGTGAGCTTA+GGG 0.444332 4.2:-6833512 None:intergenic
CATTTGAAGTTGAAAGACCT+TGG 0.447563 4.2:+6833857 MS.gene72174:CDS
ATCCAAAAGATAACTTACTT+TGG 0.452303 4.2:+6836133 MS.gene72174:CDS
TCCTTGTATTGCAGCTTCTA+CGG 0.457600 4.2:-6834940 None:intergenic
TCTAGTTCTTTATTAGAACT+TGG 0.460495 4.2:-6834447 None:intergenic
GACCTCATGGAACTCAAATT+CGG 0.460663 4.2:+6833471 None:intergenic
CTTAATCAAATCTTCTGCCT+TGG 0.461307 4.2:-6835308 None:intergenic
ACCAGATAAACTCTGCTCTT+GGG 0.462112 4.2:+6837632 MS.gene72174:CDS
CGTCGAAACAGTGATGAATC+AGG 0.465924 4.2:+6835127 MS.gene72174:CDS
AGTTAGTAACTTGACCTTGT+TGG 0.472182 4.2:+6834242 MS.gene72174:CDS
ATTTGAAGTTGAAAGACCTT+GGG 0.477834 4.2:+6833858 MS.gene72174:CDS
AGTCGAGAAGTTGACCTTGT+TGG 0.484401 4.2:+6834410 MS.gene72174:CDS
GAAACAATCAGAGGTCCATC+AGG 0.484594 4.2:+6834857 MS.gene72174:CDS
GGGAAAAGTTGTGGAAATTG+AGG 0.484696 4.2:+6833828 MS.gene72174:CDS
TAGTGATAACAATGTTGAAA+TGG 0.489700 4.2:+6833930 MS.gene72174:CDS
GTTCTTTATTGCAACTTGGC+CGG 0.493823 4.2:-6834359 None:intergenic
GACTCTAAGGAGTTCATCAA+TGG 0.495098 4.2:-6837782 None:intergenic
ATGACATTGTTGAGGAAGCC+TGG 0.497227 4.2:+6834156 MS.gene72174:CDS
AGGAAGGCGAAGATGCTGAT+TGG 0.497752 4.2:+6835284 MS.gene72174:intron
TTCGACGACTTCCAGGATCA+AGG 0.498563 4.2:-6835111 None:intergenic
TCTACTTCTTTATTGCAACC+TGG 0.499754 4.2:-6834279 None:intergenic
TGAAATGTGGGAGCTAAACC+AGG 0.501204 4.2:-6840668 None:intergenic
AGTACTTACAACAAAACCCT+TGG 0.502520 4.2:-6835371 None:intergenic
TGCTTCCAATCTCCCATCAA+AGG 0.503013 4.2:-6837825 None:intergenic
GGCATCCAGTCAGTTTCAAA+AGG 0.506934 4.2:+6840629 MS.gene72174:CDS
TGAGTCATGGTTAAGACAGA+AGG 0.510692 4.2:+6835539 MS.gene72174:CDS
GAGACCGCTTCCCTTGATCC+TGG 0.511659 4.2:+6835100 MS.gene72174:CDS
AACTCCTTAGAGTCTGTAGT+TGG 0.511792 4.2:+6837791 MS.gene72174:CDS
ATGGTTAAGACAGAAGGGCT+TGG 0.512552 4.2:+6835545 MS.gene72174:CDS
ATCAATGTACAAGCAAAACT+TGG 0.513561 4.2:+6835572 MS.gene72174:CDS
AATTTACTAACTTCATCACT+TGG 0.516167 4.2:-6834315 None:intergenic
CCGTCTGATTTAAATTCAGT+TGG 0.522605 4.2:+6834900 MS.gene72174:CDS
TTCAATCCTTGGGGTTTGGG+AGG 0.523152 4.2:-6833892 None:intergenic
ACCGAAATCTTCTGTCAAAG+TGG 0.532098 4.2:+6835502 MS.gene72174:CDS
TTGTCCTCATTATACAAAGA+CGG 0.536396 4.2:-6834033 None:intergenic
AATGAAGCCCTGCGGAAACC+CGG 0.536701 4.2:+6833967 MS.gene72174:CDS
TTAAATCAAATAGTTCCTGA+TGG 0.542304 4.2:-6834872 None:intergenic
GATTAGCACCTACATCAATT+GGG 0.542482 4.2:-6834746 None:intergenic
TCAAGTTCTTTATTGCAACT+TGG 0.544444 4.2:-6834363 None:intergenic
GTTTATTATCACCTTGGCAA+GGG 0.544529 4.2:-6833523 None:intergenic
TGAGGACAAAGACGAGGATG+AGG 0.544633 4.2:+6834047 MS.gene72174:CDS
CGTAGTAAGCTGCAATGCCA+AGG 0.547738 4.2:+6835354 MS.gene72174:CDS
GCTTCCAATCTCCCATCAAA+GGG 0.548225 4.2:-6837824 None:intergenic
AACATTTCCGGGTTTCCGCA+GGG 0.548649 4.2:-6833974 None:intergenic
CAATCTTAAAATAATTAGAT+GGG 0.548668 4.2:-6836433 None:intergenic
TTGCAACAGCCACAAAGAGC+AGG 0.549085 4.2:+6834678 MS.gene72174:CDS
TGTTTATTATCACCTTGGCA+AGG 0.550091 4.2:-6833524 None:intergenic
AAATTTGAGACTTCATCACT+TGG 0.552757 4.2:-6834564 None:intergenic
TTCATCAATCAGAAATATCG+TGG 0.553101 4.2:+6836396 MS.gene72174:CDS
CCTTCACATATGGAGAATGA+TGG 0.553944 4.2:+6835645 MS.gene72174:CDS
AAGATAAGATGCATCAGGGT+TGG 0.554730 4.2:-6833773 None:intergenic
GACTGGAGATAGAGTTGATG+TGG 0.556420 4.2:+6835330 MS.gene72174:CDS
TCCGTAGAAGCTGCAATACA+AGG 0.561934 4.2:+6834939 MS.gene72174:CDS
AAACAAACCTCAAAAGCAGT+TGG 0.562077 4.2:+6835073 MS.gene72174:CDS
TTCTACGGACAACTCAGTCT+TGG 0.562646 4.2:-6834925 None:intergenic
AATGCAGAGTGGACAAGTGA+AGG 0.565174 4.2:+6834119 MS.gene72174:CDS
CAAAGACGAGGATGAGGACG+TGG 0.565545 4.2:+6834053 MS.gene72174:CDS
CCGGTGTGGCTGTTCTAACA+AGG 0.566614 4.2:-6834340 None:intergenic
CTGTTTGGTACAGGTTGTAG+AGG 0.567539 4.2:+6837600 MS.gene72174:intron
TCTTTGTGGCTGTTCCAACA+AGG 0.567751 4.2:-6834508 None:intergenic
GAGTCATGGTTAAGACAGAA+GGG 0.568767 4.2:+6835540 MS.gene72174:CDS
CCAACTGAATTTAAATCAGA+CGG 0.568819 4.2:-6834900 None:intergenic
GAGACCATGAACCCTTTGAT+GGG 0.569999 4.2:+6837813 MS.gene72174:CDS
TGAAAAGAAGAGAAATCTCA+TGG 0.574447 4.2:+6835971 MS.gene72174:CDS
TCGACGACTTCCAGGATCAA+GGG 0.574801 4.2:-6835110 None:intergenic
CCATCATTCTCCATATGTGA+AGG 0.575054 4.2:-6835645 None:intergenic
CCCTAAGCTCACCCTTGCCA+AGG 0.575869 4.2:+6833512 MS.gene72174:CDS
AAGTTTGCGACTTCATCACT+TGG 0.578380 4.2:-6834648 None:intergenic
CATACGTGTTACTATCAGAC+AGG 0.579563 4.2:+6835895 MS.gene72174:CDS
TTATTAGAACTTGGTCTGTG+TGG 0.580255 4.2:-6834438 None:intergenic
TTCACTAAGACCAAAAGAGA+AGG 0.583737 4.2:+6835938 MS.gene72174:CDS
AACTTCTCGACTTCATCACT+CGG 0.585095 4.2:-6834399 None:intergenic
TTGGAACAACCACACAGACC+AGG 0.585459 4.2:+6834261 MS.gene72174:CDS
TTTATCATTAAAGGAGGTCA+CGG 0.589002 4.2:+6837663 MS.gene72174:CDS
ATCAGAAAACAAAATACAAG+AGG 0.589419 4.2:+6841242 MS.gene72174:CDS
CTGTTGTTAATGCAGGTTGA+AGG 0.600793 4.2:+6836359 MS.gene72174:intron
AGCAGCTCAAACAGATGTAG+AGG 0.601853 4.2:+6837844 MS.gene72174:CDS
AAGTTACTAACTTCATAACT+CGG 0.605666 4.2:-6834231 None:intergenic
TTATTGCAACTTGGCCGGTG+TGG 0.606693 4.2:-6834354 None:intergenic
ACTTCCAGGATCAAGGGAAG+CGG 0.612886 4.2:-6835104 None:intergenic
GATTTGGATTTCAATCCTTG+GGG 0.619602 4.2:-6833901 None:intergenic
TTTGACAGAAGATTTCGGTA+TGG 0.621714 4.2:-6835498 None:intergenic
AAAGCCGTCTTTGTATAATG+AGG 0.622243 4.2:+6834029 MS.gene72174:CDS
ATTGTTGAGGAAGCCTGGAT+CGG 0.623282 4.2:+6834161 MS.gene72174:CDS
TTATTGCAACCTGGTCTGTG+TGG 0.626358 4.2:-6834270 None:intergenic
TGGCAGGCTAGACTTCAAGT+TGG 0.627339 4.2:+6836091 MS.gene72174:intron
AGGACGTGGACATGTCTTCG+AGG 0.631742 4.2:+6834067 MS.gene72174:CDS
TCTGTGTGGTTGTTCCAACA+AGG 0.634295 4.2:-6834256 None:intergenic
TCATCAATCAGAAATATCGT+GGG 0.638027 4.2:+6836397 MS.gene72174:CDS
CCTTGTTAGAACAGCCACAC+CGG 0.640177 4.2:+6834340 MS.gene72174:CDS
AAATTACCTCCCAAACCCCA+AGG 0.648859 4.2:+6833886 MS.gene72174:CDS
TATCAAGATAAGATGCATCA+GGG 0.650570 4.2:-6833777 None:intergenic
GTAGTAAGCTGCAATGCCAA+GGG 0.651430 4.2:+6835355 MS.gene72174:CDS
TGTGTCCATACCTGAAATGT+GGG 0.654296 4.2:-6840680 None:intergenic
ATGTGTCCATACCTGAAATG+TGG 0.655457 4.2:-6840681 None:intergenic
TTGTCTCTTAAAATGCAGAG+TGG 0.657453 4.2:+6834108 MS.gene72174:CDS
TCTGTGTGGCTGTTCCAACA+AGG 0.658864 4.2:-6834424 None:intergenic
ACAGAAGCAGAAACAATCAG+AGG 0.662006 4.2:+6834848 MS.gene72174:CDS
CGAAGATGCTGATTGGACCA+AGG 0.664461 4.2:+6835291 MS.gene72174:CDS
GTTTGGAGATGTGAGAGTCG+TGG 0.672097 4.2:+6834722 MS.gene72174:CDS
TTAGTGACATGACATTGTTG+AGG 0.681631 4.2:+6834148 MS.gene72174:CDS
AATATCGTGGGATTCCACGT+TGG 0.686793 4.2:+6836409 MS.gene72174:CDS
TTGAAAGTGAATTGAAGACG+CGG 0.705451 4.2:+6834199 MS.gene72174:CDS
GAAATGTGGGAGCTAAACCA+GGG 0.713750 4.2:-6840667 None:intergenic
ATAATTAGATGGGTCCAACG+TGG 0.724986 4.2:-6836423 None:intergenic
GTATGGCAGAAATCCTACCA+AGG 0.737333 4.2:-6835481 None:intergenic
GTATAATGAGGACAAAGACG+AGG 0.738789 4.2:+6834041 MS.gene72174:CDS
AGAAACCTAATGAAGCCCTG+CGG 0.790697 4.2:+6833959 MS.gene72174:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr4.2 gene 6833477 6841439 6833477 ID=MS.gene72174
chr4.2 mRNA 6833477 6841439 6833477 ID=MS.gene72174.t1;Parent=MS.gene72174
chr4.2 exon 6833477 6833533 6833477 ID=MS.gene72174.t1.exon1;Parent=MS.gene72174.t1
chr4.2 CDS 6833477 6833533 6833477 ID=cds.MS.gene72174.t1;Parent=MS.gene72174.t1
chr4.2 exon 6833757 6834755 6833757 ID=MS.gene72174.t1.exon2;Parent=MS.gene72174.t1
chr4.2 CDS 6833757 6834755 6833757 ID=cds.MS.gene72174.t1;Parent=MS.gene72174.t1
chr4.2 exon 6834837 6834973 6834837 ID=MS.gene72174.t1.exon3;Parent=MS.gene72174.t1
chr4.2 CDS 6834837 6834973 6834837 ID=cds.MS.gene72174.t1;Parent=MS.gene72174.t1
chr4.2 exon 6835057 6835177 6835057 ID=MS.gene72174.t1.exon4;Parent=MS.gene72174.t1
chr4.2 CDS 6835057 6835177 6835057 ID=cds.MS.gene72174.t1;Parent=MS.gene72174.t1
chr4.2 exon 6835286 6835384 6835286 ID=MS.gene72174.t1.exon5;Parent=MS.gene72174.t1
chr4.2 CDS 6835286 6835384 6835286 ID=cds.MS.gene72174.t1;Parent=MS.gene72174.t1
chr4.2 exon 6835468 6835767 6835468 ID=MS.gene72174.t1.exon6;Parent=MS.gene72174.t1
chr4.2 CDS 6835468 6835767 6835468 ID=cds.MS.gene72174.t1;Parent=MS.gene72174.t1
chr4.2 exon 6835885 6835992 6835885 ID=MS.gene72174.t1.exon7;Parent=MS.gene72174.t1
chr4.2 CDS 6835885 6835992 6835885 ID=cds.MS.gene72174.t1;Parent=MS.gene72174.t1
chr4.2 exon 6836097 6836168 6836097 ID=MS.gene72174.t1.exon8;Parent=MS.gene72174.t1
chr4.2 CDS 6836097 6836168 6836097 ID=cds.MS.gene72174.t1;Parent=MS.gene72174.t1
chr4.2 exon 6836374 6836460 6836374 ID=MS.gene72174.t1.exon9;Parent=MS.gene72174.t1
chr4.2 CDS 6836374 6836460 6836374 ID=cds.MS.gene72174.t1;Parent=MS.gene72174.t1
chr4.2 exon 6837613 6837684 6837613 ID=MS.gene72174.t1.exon10;Parent=MS.gene72174.t1
chr4.2 CDS 6837613 6837684 6837613 ID=cds.MS.gene72174.t1;Parent=MS.gene72174.t1
chr4.2 exon 6837776 6837865 6837776 ID=MS.gene72174.t1.exon11;Parent=MS.gene72174.t1
chr4.2 CDS 6837776 6837865 6837776 ID=cds.MS.gene72174.t1;Parent=MS.gene72174.t1
chr4.2 exon 6840584 6840691 6840584 ID=MS.gene72174.t1.exon12;Parent=MS.gene72174.t1
chr4.2 CDS 6840584 6840691 6840584 ID=cds.MS.gene72174.t1;Parent=MS.gene72174.t1
chr4.2 exon 6841195 6841263 6841195 ID=MS.gene72174.t1.exon13;Parent=MS.gene72174.t1
chr4.2 CDS 6841195 6841263 6841195 ID=cds.MS.gene72174.t1;Parent=MS.gene72174.t1
chr4.2 exon 6841431 6841439 6841431 ID=MS.gene72174.t1.exon14;Parent=MS.gene72174.t1
chr4.2 CDS 6841431 6841439 6841431 ID=cds.MS.gene72174.t1;Parent=MS.gene72174.t1
Gene Sequence

>MS.gene72174

ATGGAACTCAAATTCGGTCGTTTACTTGGTGAAGACCCTAAGCTCACCCTTGCCAAGGTGATAATAAACATATTATTGTATTTTTCAATTTTGTTCTGTTTTTTTTTTTTTTTTGTCTGACAAACACCACCTGAAAATTGTGTTGTCCCCGTTTAAACCCGACCGGGTTTAGCAATATCGACAAATCATCATTGCCAGTTGAGCTCGGCCTTATAGACCAAATTTGCAAGTTTCATTTATTTATTGGAATGATTATGTTTCAATATAATTGTTGTTTTAGGTATTGGGAAGAAAGGCCAACCCTGATGCATCTTATCTTGATATTGAGAAATCATTTTATAAAAGAAAAAAGGGAAAAGTTGTGGAAATTGAGGAACTTCCATTTGAAGTTGAAAGACCTTGGGCAAAAAAATTACCTCCCAAACCCCAAGGATTGAAATCCAAATCTGATGATAGTGATAACAATGTTGAAATGGAGATTAAGAAACCTAATGAAGCCCTGCGGAAACCCGGAAATGTTAGAAAAAGTAATGTTCCTAATGTTATTTTGAGAAAGCCGTCTTTGTATAATGAGGACAAAGACGAGGATGAGGACGTGGACATGTCTTCGAGGTTGAGAATGAAACCAAATTTGTCTCTTAAAATGCAGAGTGGACAAGTGAAGGAGAAGTTTAGTGACATGACATTGTTGAGGAAGCCTGGATCGGTAATTGGTAAGAATGTTGAAAGTGAATTGAAGACGCGGAAAGAAGAGCCGAGTTATGAAGTTAGTAACTTGACCTTGTTGGAACAACCACACAGACCAGGTTGCAATAAAGAAGTAGAGAAGTCTGCAAAACCAAGTGATGAAGTTAGTAAATTGACCTTGTTAGAACAGCCACACCGGCCAAGTTGCAATAAAGAACTTGAGAAGTTTGCAGAGCCGAGTGATGAAGTCGAGAAGTTGACCTTGTTGGAACAGCCACACAGACCAAGTTCTAATAAAGAACTAGAAAAGTTTGCTGAGCCAAGTGATGAAGTCGCAAAATTAACCTTGTTGGAACAGCCACAAAGAGCAAGTAGCAAAGAAGAAGAGCAGTTTGGAGAGCCAAGTGATGAAGTCTCAAATTTGACCTTGTTGGAAAAGCCACAAAGTGCAAGTGACAAAGAAGAAGAAGAGCAGTTTGAAGAGCCAAGTGATGAAGTCGCAAACTTGACATTGTTGCAACAGCCACAAAGAGCAGGCGACAAAGTAGAAGAAGAGAAGTTTGGAGATGTGAGAGTCGTGGTCCCAATTGATGTAGGTGCTAATCTTTGTTGCAATTATGGAATTTGATAACGGAAACATAGTTTTAAAATTATTTCATGCTTGATTAATTAGGGATCAGAGCAACAGAAGCAGAAACAATCAGAGGTCCATCAGGAACTATTTGATTTAAATCAACCGTCTGATTTAAATTCAGTTGGTTCCAAGACTGAGTTGTCCGTAGAAGCTGCAATACAAGGAAAGCCAAAAAGGTATATGATAATAATTTCACAGTTGTATATTTCAGATTATGGTTGCAAATATGTTTCATTACCTAAATGTAATTTTGTTATAGATTAGATCCATATGAGAAACAAACCTCAAAAGCAGTTGGAGAAGAGACCGCTTCCCTTGATCCTGGAAGTCGTCGAAACAGTGATGAATCAGGAAATCTTGTTGATGTGTTAGATATTCAGGTCAGATTTTGTGGATTACCCTAGTTACTTACTTATCTATATGAGACATTAGCAATGCATCTTCCTAAATTCCATGTTTATTTACCTTTTTGTTTTTTGATATTGTAGGAAGGCGAAGATGCTGATTGGACCAAGGCAGAAGATTTGATTAAGACTGGAGATAGAGTTGATGTGGACGTAGTAAGCTGCAATGCCAAGGGTTTTGTTGTAAGTACTTAAAAAACAGTAAAAGGGACTTTCATATATAGATAAAAATTAGTCTGTCTCATTTATCTTCTGACCATTTACAGGTTTCTTTTGGTACCTTGGTAGGATTTCTGCCATACCGAAATCTTCTGTCAAAGTGGAAATTTTTAGCTTTTGAGTCATGGTTAAGACAGAAGGGCTTGGATCCATCAATGTACAAGCAAAACTTGGTCACTGTGACAAATTATGATGCTGAAAATAGTAATTTTTCTGCTGATTCACCTTCACATATGGAGAATGATGGTAAACTTGAAGATAAAATTTCACCAGATATGAAATTGGAAGATCTGTTAAAGATTTATGACCAAGAGAAAATCAAGTTCTTATCATCATTTATTGGACAGGTTTGGTATTCAGAACACAAGTACACAACCCAAATAAATTTATCTATTTTTGCTGCAAAAGGTGAAATTTTAATATTATCATTCTTAATATTGAGATGCCAATACTTTGTTTTGCAGCAAATCAAAGCATACGTGTTACTATCAGACAGGAAATTGAAAAAGCTCATATTTTCACTAAGACCAAAAGAGAAGGAAGAACTAACTGAAAAGAAGAGAAATCTCATGGTAAATGCTTTAGTGACATAACTATTCTTGAATTAGCCCGGGGCAGGTGTTTTAGATTGAATGCAACAAAGTGGTATTCAGATTCGTTGTGTTTCTTATGGCAGGCTAGACTTCAAGTTGGTGATATTGTGAAATGCCGCATCCAAAAGATAACTTACTTTGGAATTTTTGTTGAGGTATATAAAACATTTGATTTCAGTATTGTGCAGTTCACTTTTACAAACATGTTTAGCTTTGCATCATTAAGCCAGCAGAAGTTCCTTGGTTAGCCTATTTTTGTTCCACAGTCTCTGTATTTAGCTAGTGCCTGGATATTCGTTCATGGAATCAAGTTCGGAGAGGTGGCAGAAAATTCTAAATATTGACCTGTTGTTAATGCAGGTTGAAGGAGTTTCTGCACTAATTCATCAATCAGAAATATCGTGGGATTCCACGTTGGACCCATCTAATTATTTTAAGATTGATCAGGTATTTTACTTCTTGACGGGGTGACAAATTAAAGTTTGCTTGTTTAAACTTCAAAAAATGTTATTCTACAATACAATTATTTTAAGATATTTTATATTTATTTTGGAGAAGTTAATATGTTTACTTTTTTGCTAATGAGAAAAGAATTGAAACTGTTTTTTCTTTACACATTTTTGCTTTTAAAGAACTTACATAGTTTTTTCGTAAACACGTCTTTTTAAAAAACACATAAGATAATGGTTATCAAATTGAGATTCATATCGTGAATCATAAGAACTGTTTTCTGAATCATGAAACATAAGAATTGTTTTCCGAATCGTGAATCCAGTCTTATTATGATTTGCAAGATACATTACTAATAAAAATTTGATATTGTTAAGAGAAAACAAGTGACATAGCTTAACTGTGATAATCCGTTTGGTCGTAAATATAAGTATAAAGCTACATGTATTTGGTCCAATTTAGATCAAATACATGTACGTTTTTGCTTTTATTTTGGACAAGAGGGAGTATATCATATTCAAACCTCATCATAATTTAAGATTCAAGTCATAAATCAAATGACAAAAGAAAAGTGGATGATTATACAAAGTTGACAAAAAGAAGTGGCTGACTACACTAAGTTTAAGTAGCAACTCAATAGAATGAATCAAGATTCTTTATTGTGAACCGGGATTTAATATGAGGTTCATGTCCAAAATAGATACACACTAGAAATTTGTATCGATTGGACTGAGATACGATACACTTTAAGATACTTTAAAATAATACTGAGATATTATAAGACATTTTCATATCTTCTAGTTCTAGCAATTTACCCAGTTATAATTCTCTTGAACACATTTTAAAAATATTTTTGCTTAAAAAAAAGAACAAACAAATTGGACTTTTTTTTTTGCCGGTGTCCGGGGTTTGAACCCACGACCTTTCCATATATTATGCATTGTCCCTACCAACTGAGCTAAGCTCACGAGGACAAACAAATTTGACTTTAATGAAGTGCAAAAAAATATATTTGGTTTGCTTCCATTATTTTGCAATCTATTACTGGCTTGGCGCTTTCAATATGGATGGAATCAATTGAGTACCCATGGAATCTGATGTTTTTATATATTACTTCTTTTGTGTTTATCTTTCTACTGTTTGGTACAGGTTGTAGAGGCAAAAGTTCACCAGATAAACTCTGCTCTTGGGCGCATATTTTTATCATTAAAGGAGGTCACGGTAGTTATCTATATAGTTATTTTGGCAGAATCTATGTAAACTATGCAGTATTTGACTCTTGATTCAATGAATCATTGCCTTCCTATTGCAGTGTGATCCATTGATGAACTCCTTAGAGTCTGTAGTTGGAGACCATGAACCCTTTGATGGGAGATTGGAAGCAGCTCAAACAGATGTAGAGGTAATCTTTACATGAAAATAGAAGTAACTTTAAATTCAAAAAAAGAAAAATCGACGTAGACCTATTGAATTTATTAAATAACTGTGTTGTCTATATTACAAAATATATATATATATATATATATATATAGAGGCTGCTAAGTTACACCCACCTAAAAACTCTAAGGTGTAAGTTAGCAAGCTACACCCACCTAAAATTATTAAGGTGCAAGTTAGCAACCTACACCCACTTTATTATTTACACTCATTTTTATAAGTATTATTAAGGTGCAAGTTAGCAACCTTCACTAATTTACACCCACTTTCATCATTCCAAATATTGTTTCCTTAAAAGTTTGTCTTTATTACATGTTTTGTACCTTAAGCATGTCATTGTCTTTTTTGTTTCTTTTTTTAAGGATCTTTTTTAAGCTAGTTACAAAATTGCCCTTCTATTTAAAATTAATTAGAATATAAACAAAAGGGTGTTTTTGTAAATTATAAGTGGGTGCAGCTTGCTAAGTTAGCAGCCCCCTATATATATATATATATATATATATATATATATATATATATATACATACACGTGTTTTATTTGGAAGGATGGAGCTGACCCCTGCAAAATAAAAAAAATTATTGAATGATACCCAAGATAGATAGAGAAATATGTATTATTGAGTTAAAAGGAAAACAGCCTTACAGCCAATTCGTCAATACACATTGCTAATTCACAGATACCGTTTATGGTTACCCTACTCTCTATCTAGTGTCCATTCTATTATTCCCTTTTCTACCCCACGTGTATGTTTGCTCGTCGAATTAATAATAGATCTATTATTAATAATAATGGGCCTATCAGTCAATAACAAGCTGCTAGAGTGAATTTGGTCATTTCTGCTGATGGTAGTGATAAATCTACGGGTCTTTGTGTGGGAATAACTATAAGAATTGAATAAAGTTCAAAATACAACTAATAGGTGGTGGCTTAAATGGTAAAGTTCACTCCCATGAAGTTTGGTGATAATAAATTGGGTTTGATTATGTGAAGTGTGAAAATGGGGCAAGGCAACTTATACTCCATTGCCTTTGGTTAAGTGAGTTTGGATTATGGTAAGACCCACCTTGGTGGTAGGCATGGCAACAGAACCCGTACCCATGGGTATCCACCCCAACTTTGACGGGAAAACTTGAATTGACTGGGTTTAAGTTTTCCCCGATTTAAAAAGACGGGGGCGGGTAATGTGGACACTAATGCTCACCCCAAACCCGCCCCGCTTGTATCAAAATTACATTTTTACCTCTATTAAATTAGTAACCTTGAAACAAACCCTTAAATTACATTCATATATTTATTTTTATTTTAAAGATGTGTCTTAACTAAGTCGTTTTCTCTCTCTTTTTTTTTTTTTTGGTTTACAAAAATCAGATTGTTGGAATGGCGTAAGTTTGCACTTTCGGAATTCTTTTACAAAATGCGGTCAAAATTCATGTGGGGGTGGGGTGGGGATACCCGAACCTGTCGGGTATGGGGCTGGGGTTCTATTTTCACCCCTGTTGGGAATTGGTATTGGTACAGGGATACCAGAACTCCATTGGGTTTGGGGACAGGGATACCTAAGTGTACAAGTTTTTCTGAATTTTGTAGTTTGTGACCACATTTTGTAAAAAACTGAGGGTAAAAGTAAGTAAAGTAGTGGTTCCGATGTGTTGTTGGATGTTGAAATTTGGGGTCTAACTTATCCTTACAAAACCGGCTTGTAAGATGAGGAGTGTCTCCTCTTTATAACTCTTCTCAAGAGTCCTATCTATCCAATGTGGGACTAAATACACCCCTCAAAGCCAACACAACGAAGGTGTCGGAGGCTGCAATGAAGGCAAACCAAAGGCCGCAATTTAGGCGACGAGGAGTGGACCGCAATAAAAGCGGAATTTCCAACACACCCTCACGCTTCAGCCCGGCCCAATGGGTCGGAGGCTGCAATGAAGGCAAACCAAAGGCCGCAATTTAGGCGACGAGGAGTGGACCGCAATAAAAGCGGAATTTCCAACACACCCTCACGCTTCAGCCCGGCCCAATGGGTCTGAAGCGTGGACAATGCGGGAAGCCCAACGATAGATCTAGGATAGGCTCTGATACCATGTTGAAATTTGGGGCTTAACTCATCCTTACAAAACCGGCTTGTAAGTTGTGGAGTGCCTCCTCTTTATAAACTCTTCTCAAGAGTCTCATCTATCTGATGTGGGACTAAGTCCACCCCCCTCAAAGCCAACACAATGAAGGTGTTGGAGGCTGCAATGAAGGCAAACCTAAGGCCGCAATTTAGGTGACTAGGGGCGGACCGCAATAAATGCGGAATTTCCAGCATTTGATAAATCAAGTTTTTTATTTATTTTTTAAACCGGTTCAGTGTCTGAAGGTACATAATTTGCAGTACAGTATGAAAATATAATTTTGAAATAATGAATGTTTTCCGCATTTTGGTTCCGCTATTCAAGTTTTTTTGTTTTTTTTTAACAAGATATTCAAGTTCTGACTTTTGGCCCTTTGGTTATGCCTGCATTCTGTGCATAGTATTTTCATTTCAATCAATCATTTCGTTCAGACGCAGTTTGTGATGTAGTAGATAAATAATTATTCTTGCCCTACAATGAATCAAAGATAAGTATGCTACATTTATTTTACTGTCTCGTTGATTTGTGGCTTCGAATGATTAATCATGTTATGCTCGTTTTTGGACAGTGGTCTGAAGTCGAATCTCTTATAAAAGAATTGCAAAAAATTGAGGGCATCCAGTCAGTTTCAAAAGGCCGTTTTTTCAGGAGCCCTGGTTTAGCTCCCACATTTCAGGTATGGACACATTCAAACCTCTGCATAATATAATGAATCATATTTGGATTTGCATTTTATTATTTACTTGAAAGAATATTAGTTCATTTTTTATTTTGTTGATTGAGCTGGCATTATGAATCATGGATTCCCCGTATGGTTTATGAAATGTTAAAATGAATCAACTGTCAGATATAATATGGTTGTCTTATAAACTTTACTATTCAACTAGAAAACCATTTCTGTCAAATTATTCTTTTTCCATTTTCCCTTTGAAGATTTAATTAATTGTTCTTGTTTTGAGCTGTCACTACCAGACTATTGAAAAACACGTATAAATTATTGTAATTCTAAAAAAAACTTGGCTAATGCGTAGACTAACTCTTTTTAGTTTTTCTGAAACCTTCCATGTTCATAAATAACGTATGGGAAGCTATTTAATGTTTGGTTCTTGAAACAGTTTAATATTTGTTAGCAAACTCAAGTTCTTGATTTATGTGCATGCAAACCAAATTTTGTTCCAGGTTTATATGGCATCCATATTTGAGAATCAGTACAAGTTGCTTGCCCGATCAGAAAACAAAATACAAGAGGTAATGTTGGTCTTAGTCTGTTTTAGCTTCTTGTTAAACATATTTTGCTGTATCATTCAAGATTGTCTCCCCTCTCATTTGTATTCCTTTTTCTCTTTATATTAATGACGAATTTTGAGTTATATATACTTAATGATATTGCTTTTTTAAATGACTTTTCATGTTAGAAATACTAG
Protein sequence

>MS.gene72174.t1

MELKFGRLLGEDPKLTLAKVLGRKANPDASYLDIEKSFYKRKKGKVVEIEELPFEVERPWAKKLPPKPQGLKSKSDDSDNNVEMEIKKPNEALRKPGNVRKSNVPNVILRKPSLYNEDKDEDEDVDMSSRLRMKPNLSLKMQSGQVKEKFSDMTLLRKPGSVIGKNVESELKTRKEEPSYEVSNLTLLEQPHRPGCNKEVEKSAKPSDEVSKLTLLEQPHRPSCNKELEKFAEPSDEVEKLTLLEQPHRPSSNKELEKFAEPSDEVAKLTLLEQPQRASSKEEEQFGEPSDEVSNLTLLEKPQSASDKEEEEQFEEPSDEVANLTLLQQPQRAGDKVEEEKFGDVRVVVPIDGSEQQKQKQSEVHQELFDLNQPSDLNSVGSKTELSVEAAIQGKPKRLDPYEKQTSKAVGEETASLDPGSRRNSDESGNLVDVLDIQEGEDADWTKAEDLIKTGDRVDVDVVSCNAKGFVVSFGTLVGFLPYRNLLSKWKFLAFESWLRQKGLDPSMYKQNLVTVTNYDAENSNFSADSPSHMENDGKLEDKISPDMKLEDLLKIYDQEKIKFLSSFIGQQIKAYVLLSDRKLKKLIFSLRPKEKEELTEKKRNLMARLQVGDIVKCRIQKITYFGIFVEVEGVSALIHQSEISWDSTLDPSNYFKIDQVVEAKVHQINSALGRIFLSLKEVTCDPLMNSLESVVGDHEPFDGRLEAAQTDVEWSEVESLIKELQKIEGIQSVSKGRFFRSPGLAPTFQVYMASIFENQYKLLARSENKIQEKY