AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0105470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0105470 MtrunA17_Chr1g0160431 87.255 204 25 1 77 279 33 236 6.67e-111 320
Msa0105470 MtrunA17_Chr8g0372791 35.938 192 106 4 91 279 48 225 1.29e-17 79.7
Msa0105470 MtrunA17_Chr6g0453501 30.350 257 137 8 28 279 36 255 3.03e-13 68.2
Msa0105470 MtrunA17_Chr1g0160441 85.294 34 5 0 77 110 33 66 1.23e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0105470 AT2G43630.1 36.316 190 104 3 100 279 80 262 1.16e-30 116

Find 97 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCGCTTGTTCAATGTTTC+TGG 0.075211 1_3:+13986024 None:intergenic
AAATGCAATGGACAAATTTA+AGG 0.142467 1_3:-13984550 Msa0105470:CDS
TAAGCGTATCATGGTAAAAT+TGG 0.154842 1_3:-13983667 Msa0105470:CDS
ATCTGGTGCCTTACTCTTTC+TGG 0.178984 1_3:-13984346 Msa0105470:intron
GAAACTCCGGCGGCGGTTTC+CGG 0.199718 1_3:+13986056 None:intergenic
ATAAGAGATAAACCGTTATT+TGG 0.220474 1_3:-13985700 Msa0105470:CDS
ATCCACTTTGGTGCCATTAA+TGG 0.233740 1_3:+13986285 None:intergenic
CGCAAAACAGACTGGATAAC+TGG 0.329265 1_3:+13986259 None:intergenic
CCAGGAGGAGGAGGAGAAGG+TGG 0.330061 1_3:-13984447 Msa0105470:CDS
GGTGCCATTGGTGCCATTAA+TGG 0.332681 1_3:-13986298 Msa0105470:CDS
AAGGACAGTTGTAACTTCTA+AGG 0.333710 1_3:-13983349 Msa0105470:three_prime_UTR
CCATGTTGGGGTATTCAAAA+TGG 0.340619 1_3:+13983584 None:intergenic
AATCACTGATGAGCACTTCT+TGG 0.357933 1_3:-13983613 Msa0105470:CDS
CAGGATCATACCACCATGTT+GGG 0.367552 1_3:+13983571 None:intergenic
CACCAGATGCGAAAATTGTT+TGG 0.372513 1_3:+13984361 None:intergenic
AAACATTGAACAAGCGCTTC+AGG 0.381975 1_3:-13986020 Msa0105470:intron
AATGCAATGGACAAATTTAA+GGG 0.386519 1_3:-13984549 Msa0105470:CDS
CCACCTTCTCCTCCTCCTCC+TGG 0.389125 1_3:+13984447 None:intergenic
TTACGGAAGCAAATTCAAAA+AGG 0.403502 1_3:-13984507 Msa0105470:CDS
TTAAGGTTTATAGTTGGCTT+AGG 0.406725 1_3:-13983705 Msa0105470:CDS
AGGAGGAGGAGGAGAAGGTG+GGG 0.423587 1_3:-13984445 Msa0105470:CDS
CCTCTTCGTGATGGTGGAAA+AGG 0.427247 1_3:-13985661 Msa0105470:CDS
TATTGTGAATTTGTCCCTAC+TGG 0.427513 1_3:-13983249 Msa0105470:three_prime_UTR
GGTGGTGGTGGCAGTCCCAA+TGG 0.428342 1_3:-13984423 Msa0105470:CDS
AAAGATTTCAATCCAAATAA+CGG 0.429165 1_3:+13985688 None:intergenic
GGTGGCAGTCCCAATGGCCC+TGG 0.434107 1_3:-13984417 Msa0105470:CDS
TTCAATGTTTCTGGCGCCGA+CGG 0.444111 1_3:+13986033 None:intergenic
AAGCGTATCATGGTAAAATT+GGG 0.456666 1_3:-13983666 Msa0105470:CDS
GTGTGTGGTGGAGATGGAAT+TGG 0.457029 1_3:-13986323 None:intergenic
GGAGGAGGAGGAGAAGGTGG+GGG 0.460530 1_3:-13984444 Msa0105470:CDS
TATTTAGACAATGGTGGAAG+TGG 0.463016 1_3:-13984480 Msa0105470:CDS
CCACCAGGAGGAGGAGGAGA+AGG 0.467905 1_3:-13984450 Msa0105470:CDS
AATCAATAGAAGAAGTATTA+CGG 0.472780 1_3:-13984524 Msa0105470:CDS
GCCTCAAGTGGTTTGTCTAG+TGG 0.478776 1_3:-13983296 Msa0105470:three_prime_UTR
TCAGATCCACCAGGGCCATT+GGG 0.486936 1_3:+13984408 None:intergenic
ATCAGATCCACCAGGGCCAT+TGG 0.489944 1_3:+13984407 None:intergenic
AGAGTGTACCAGAAAGAGTA+AGG 0.496063 1_3:+13984338 None:intergenic
GATGGTGGAAAAGGAATGTC+AGG 0.498530 1_3:-13985652 Msa0105470:CDS
GTAAAGCCACCAGGAGGAGG+AGG 0.507231 1_3:-13984456 Msa0105470:CDS
CATTTGTAATAGATTGCCAT+GGG 0.513734 1_3:+13984568 None:intergenic
CAGGAGGAGGAGGAGAAGGT+GGG 0.514543 1_3:-13984446 Msa0105470:CDS
TTGAAGCCTCTTCGTGATGG+TGG 0.523422 1_3:-13985667 Msa0105470:CDS
GGAGTAAAGCCACCAGGAGG+AGG 0.524624 1_3:-13984459 Msa0105470:CDS
CCGACGGGAAACTCCGGCGG+CGG 0.525013 1_3:+13986049 None:intergenic
ATGGAATTGGATGGTGCCAT+TGG 0.532731 1_3:-13986310 Msa0105470:CDS
TCAATGTTTCTGGCGCCGAC+GGG 0.533034 1_3:+13986034 None:intergenic
TCAGGATCATACCACCATGT+TGG 0.534231 1_3:+13983570 None:intergenic
GCATTTGTAATAGATTGCCA+TGG 0.534840 1_3:+13984567 None:intergenic
GAGTTATTCGTCTGCATCTG+AGG 0.535731 1_3:+13983515 None:intergenic
GGCAGTCCCAATGGCCCTGG+TGG 0.545700 1_3:-13984414 Msa0105470:CDS
GCAATCTATTACAAATGCAA+TGG 0.555704 1_3:-13984562 Msa0105470:CDS
TAGTCGAATTAAGCGTATCA+TGG 0.558198 1_3:-13983676 Msa0105470:CDS
TAAAATTGGGAAAATTGTAC+CGG 0.563003 1_3:-13983653 Msa0105470:CDS
GGTGCCATTAATGGCACCAA+TGG 0.566409 1_3:+13986294 None:intergenic
CTGGCGCCGACGGGAAACTC+CGG 0.567046 1_3:+13986043 None:intergenic
CCTACTGGTTTCTTTGAGCG+AGG 0.568067 1_3:-13983234 Msa0105470:three_prime_UTR
GGTGAGTATTTAGACAATGG+TGG 0.570123 1_3:-13984486 Msa0105470:CDS
ATCTCACCGGAAACCGCCGC+CGG 0.570989 1_3:-13986062 Msa0105470:CDS
GGCACAACAAATGCAAGGAA+AGG 0.572946 1_3:+13983390 None:intergenic
TGATGAGCACTTCTTGGAGA+AGG 0.574676 1_3:-13983607 Msa0105470:CDS
TCTTTGAAGCCTCTTCGTGA+TGG 0.576819 1_3:-13985670 Msa0105470:CDS
CCTTCTCCTCCTCCTCCTGG+TGG 0.583094 1_3:+13984450 None:intergenic
GATAACTGGACTATCCACTT+TGG 0.584308 1_3:+13986273 None:intergenic
TAAGTATTCAACGAGTTGCA+AGG 0.585579 1_3:-13983193 Msa0105470:three_prime_UTR
AGTATCATTCTAGCCTCAAG+TGG 0.587594 1_3:-13983308 Msa0105470:three_prime_UTR
TCTTCATCATCAGATCCACC+AGG 0.590752 1_3:+13984399 None:intergenic
AAGGTAAGAAAACAAATGAG+AGG 0.591414 1_3:+13983462 None:intergenic
CCTTGCTATATGACAATCTG+AGG 0.595953 1_3:+13983368 None:intergenic
AAAGGTGAGTATTTAGACAA+TGG 0.597947 1_3:-13984489 Msa0105470:CDS
AAAGAGCATCATTAAACTCA+AGG 0.598768 1_3:+13983443 None:intergenic
AAATGCAATCGCTGCCGGTG+TGG 0.605334 1_3:-13986122 Msa0105470:CDS
ACTGCCGTACGAAAATCCTC+AGG 0.605585 1_3:+13983552 None:intergenic
CCTCGCTCAAAGAAACCAGT+AGG 0.607545 1_3:+13983234 None:intergenic
TATCTATCTCATCATGAGCA+AGG 0.607713 1_3:-13983754 Msa0105470:CDS
AGGTAAGAAAACAAATGAGA+GGG 0.608436 1_3:+13983463 None:intergenic
TGCCATTAATGGCACCAAAG+TGG 0.621150 1_3:-13986287 Msa0105470:CDS
AACATGGTGGTATGATCCTG+AGG 0.623546 1_3:-13983568 Msa0105470:CDS
CCTCAGATTGTCATATAGCA+AGG 0.623611 1_3:-13983368 Msa0105470:three_prime_UTR
TCCACTAGACAAACCACTTG+AGG 0.624133 1_3:+13983295 None:intergenic
TCGCACTGAACTCATCTCAC+CGG 0.624534 1_3:-13986075 Msa0105470:CDS
CTGAGGGCACAACAAATGCA+AGG 0.631030 1_3:+13983385 None:intergenic
GTGGTGGAGATGGAATTGGA+TGG 0.632280 1_3:-13986319 None:intergenic
GCGCCGACGGGAAACTCCGG+CGG 0.633580 1_3:+13986046 None:intergenic
CCGCCGCCGGAGTTTCCCGT+CGG 0.634582 1_3:-13986049 Msa0105470:CDS
GGAAGTGGAGTAAAGCCACC+AGG 0.635749 1_3:-13984465 Msa0105470:CDS
TAATGTGTACTCGAATCCAA+AGG 0.645425 1_3:+13983165 None:intergenic
AATGTCAGGAAAGAGTGATG+AGG 0.648815 1_3:-13985638 Msa0105470:intron
AGGATCATACCACCATGTTG+GGG 0.658146 1_3:+13983572 None:intergenic
TGTAAGCTGTTTGTTATACG+AGG 0.661610 1_3:+13986099 None:intergenic
CTTGCTATATGACAATCTGA+GGG 0.672192 1_3:+13983369 None:intergenic
ATAATAATCAGAATTCCCCA+TGG 0.676568 1_3:-13984584 Msa0105470:intron
CTCGCTCAAAGAAACCAGTA+GGG 0.676907 1_3:+13983235 None:intergenic
TTTGTTATACGAGGCCACAC+CGG 0.679217 1_3:+13986108 None:intergenic
TGTACCGGAGTACGAAACAG+AGG 0.680091 1_3:-13983638 Msa0105470:CDS
CTTCATCATCAGATCCACCA+GGG 0.689052 1_3:+13984400 None:intergenic
AGTGGAGTAAAGCCACCAGG+AGG 0.725114 1_3:-13984462 Msa0105470:CDS
ATTTGTAATAGATTGCCATG+GGG 0.741669 1_3:+13984569 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCTTTTTTTCTTTTTAAA+TGG - chr1_3:13984444-13984463 Msa0105470:CDS 10.0%
!! AAATTATAACTAAAAAGAAG+TGG + chr1_3:13983874-13983893 None:intergenic 15.0%
!!! TTTTTCTTTTTAAATGGAAA+TGG - chr1_3:13984450-13984469 Msa0105470:CDS 15.0%
!!! TAGTTAATGTTTTTGATTAA+TGG - chr1_3:13985304-13985323 Msa0105470:intron 15.0%
!!! AAAAATTTTGATGTCAAATT+TGG - chr1_3:13985536-13985555 Msa0105470:intron 15.0%
!! AAAGATTTCAATCCAAATAA+CGG + chr1_3:13983796-13983815 None:intergenic 20.0%
!!! TTTTATCTAAAGATGCATTT+AGG - chr1_3:13984138-13984157 Msa0105470:intron 20.0%
!!! TTAAGTTGTTTATACAAACA+AGG - chr1_3:13984409-13984428 Msa0105470:CDS 20.0%
!!! ATTTTTATAATAGACAACCT+AGG - chr1_3:13984630-13984649 Msa0105470:intron 20.0%
!!! TTTTTATAATAGACAACCTA+GGG - chr1_3:13984631-13984650 Msa0105470:intron 20.0%
!!! AATCAATAGAAGAAGTATTA+CGG - chr1_3:13984957-13984976 Msa0105470:intron 20.0%
!!! ATTAAGAGTTGATAGAATAT+AGG - chr1_3:13985475-13985494 Msa0105470:intron 20.0%
!!! TTTTCAAAAGGAAATGGATT+AGG + chr1_3:13983333-13983352 None:intergenic 25.0%
! ATAAGAGATAAACCGTTATT+TGG - chr1_3:13983781-13983800 Msa0105470:intron 25.0%
!! AATTTTGCCTGTTATTATTG+AGG - chr1_3:13983889-13983908 Msa0105470:intron 25.0%
!! ATTTTGCCTGTTATTATTGA+GGG - chr1_3:13983890-13983909 Msa0105470:intron 25.0%
!! TATTTTCCCTCAATAATAAC+AGG + chr1_3:13983899-13983918 None:intergenic 25.0%
!!! GAATCATGTTATTTCTCAAT+CGG - chr1_3:13984002-13984021 Msa0105470:intron 25.0%
!!! GGTTATTTTAGTTTATCTAC+TGG - chr1_3:13984175-13984194 Msa0105470:intron 25.0%
!!! TATACAAACAAGGTCTTATT+AGG - chr1_3:13984419-13984438 Msa0105470:CDS 25.0%
! TCTGTAGTACAAATATTGAA+AGG - chr1_3:13984602-13984621 Msa0105470:intron 25.0%
! CTGTAGTACAAATATTGAAA+GGG - chr1_3:13984603-13984622 Msa0105470:intron 25.0%
!!! TTGTTTGGTTTTTAAAACGT+TGG - chr1_3:13984818-13984837 Msa0105470:intron 25.0%
! AAATGCAATGGACAAATTTA+AGG - chr1_3:13984931-13984950 Msa0105470:intron 25.0%
! AATGCAATGGACAAATTTAA+GGG - chr1_3:13984932-13984951 Msa0105470:intron 25.0%
!!! TCTGAATTTTGAAAACTCAA+AGG - chr1_3:13985243-13985262 Msa0105470:intron 25.0%
! ATAATTTCTCTTCAGAAATG+AGG + chr1_3:13985506-13985525 None:intergenic 25.0%
!!! GACTTTTTAAGGTTTATAGT+TGG - chr1_3:13985770-13985789 Msa0105470:intron 25.0%
! TAAAATTGGGAAAATTGTAC+CGG - chr1_3:13985828-13985847 Msa0105470:intron 25.0%
GTGGGAATAAAAGATTTGAA+CGG - chr1_3:13983257-13983276 Msa0105470:three_prime_UTR 30.0%
AAAATGTTCCCTTTCAATCT+GGG + chr1_3:13983283-13983302 None:intergenic 30.0%
!!! TTTGGCTTTTCAAAAGGAAA+TGG + chr1_3:13983339-13983358 None:intergenic 30.0%
!!! GCATTTTTTGGCTTTTCAAA+AGG + chr1_3:13983345-13983364 None:intergenic 30.0%
ATGATAGGGGAAGAAAAATT+CGG + chr1_3:13983517-13983536 None:intergenic 30.0%
!! ATTTAGGACTGGTTTAGATT+GGG - chr1_3:13984154-13984173 Msa0105470:intron 30.0%
AGAATTTCCTAATAAGACCT+GGG + chr1_3:13984429-13984448 None:intergenic 30.0%
! GTACTACTGTTCAGTTATTT+TGG + chr1_3:13984780-13984799 None:intergenic 30.0%
ACAAAATCAGACAGTTCAAT+TGG + chr1_3:13984802-13984821 None:intergenic 30.0%
! TTGAACTGTCTGATTTTGTT+TGG - chr1_3:13984803-13984822 Msa0105470:intron 30.0%
ATAATAATCAGAATTCCCCA+TGG - chr1_3:13984897-13984916 Msa0105470:intron 30.0%
ATTTGTAATAGATTGCCATG+GGG + chr1_3:13984915-13984934 None:intergenic 30.0%
CATTTGTAATAGATTGCCAT+GGG + chr1_3:13984916-13984935 None:intergenic 30.0%
GCAATCTATTACAAATGCAA+TGG - chr1_3:13984919-13984938 Msa0105470:intron 30.0%
TTACGGAAGCAAATTCAAAA+AGG - chr1_3:13984974-13984993 Msa0105470:intron 30.0%
AAAGGTGAGTATTTAGACAA+TGG - chr1_3:13984992-13985011 Msa0105470:intron 30.0%
ACTCAAAGGAGAAAGTTAAA+TGG - chr1_3:13985257-13985276 Msa0105470:intron 30.0%
TCAAAAGTCTTCTCATATAC+CGG + chr1_3:13985404-13985423 None:intergenic 30.0%
TCAACTCTTAATCATGTCAA+GGG + chr1_3:13985467-13985486 None:intergenic 30.0%
ATCAACTCTTAATCATGTCA+AGG + chr1_3:13985468-13985487 None:intergenic 30.0%
TCATGATGAGATAGATATAC+TGG + chr1_3:13985724-13985743 None:intergenic 30.0%
! GCACAATTTTCGACTTTTTA+AGG - chr1_3:13985759-13985778 Msa0105470:intron 30.0%
! TTAAGGTTTATAGTTGGCTT+AGG - chr1_3:13985776-13985795 Msa0105470:intron 30.0%
TAAGCGTATCATGGTAAAAT+TGG - chr1_3:13985814-13985833 Msa0105470:intron 30.0%
AAGCGTATCATGGTAAAATT+GGG - chr1_3:13985815-13985834 Msa0105470:intron 30.0%
AGGTAAGAAAACAAATGAGA+GGG + chr1_3:13986021-13986040 None:intergenic 30.0%
AAGGTAAGAAAACAAATGAG+AGG + chr1_3:13986022-13986041 None:intergenic 30.0%
!! AAAGAGCATCATTAAACTCA+AGG + chr1_3:13986041-13986060 None:intergenic 30.0%
GAAAATGTTCCCTTTCAATC+TGG + chr1_3:13983284-13983303 None:intergenic 35.0%
! TTGAAAGGGAACATTTTCAC+TGG - chr1_3:13983286-13983305 Msa0105470:three_prime_UTR 35.0%
!! TGTAAGCTGTTTGTTATACG+AGG + chr1_3:13983385-13983404 None:intergenic 35.0%
CAATAATCGCCACTGAAAAA+AGG - chr1_3:13983579-13983598 Msa0105470:CDS 35.0%
ACTGAAAAAAGGCTATTACG+CGG - chr1_3:13983590-13983609 Msa0105470:CDS 35.0%
CAAGTTACAGCATTTCAACT+TGG - chr1_3:13983679-13983698 Msa0105470:CDS 35.0%
TAAACTCTTTCCCTCAGATT+CGG - chr1_3:13983749-13983768 Msa0105470:CDS 35.0%
TGCTAATATTCCGAATCTGA+GGG + chr1_3:13983762-13983781 None:intergenic 35.0%
ATGCTAATATTCCGAATCTG+AGG + chr1_3:13983763-13983782 None:intergenic 35.0%
! TCTAAAGATGCATTTAGGAC+TGG - chr1_3:13984143-13984162 Msa0105470:intron 35.0%
!! CATTTAGGACTGGTTTAGAT+TGG - chr1_3:13984153-13984172 Msa0105470:intron 35.0%
TAAGCACTTGTGAGATTGTT+TGG - chr1_3:13984200-13984219 Msa0105470:intron 35.0%
AAGCACTTGTGAGATTGTTT+GGG - chr1_3:13984201-13984220 Msa0105470:intron 35.0%
!! GTTGTTTTCATCAGCTACAA+TGG + chr1_3:13984284-13984303 None:intergenic 35.0%
TAATATTTACTCCTTCGTCC+TGG - chr1_3:13984499-13984518 Msa0105470:CDS 35.0%
AATTTCCTAATAAGACCTGG+GGG + chr1_3:13984583-13984602 None:intergenic 35.0%
GAATTTCCTAATAAGACCTG+GGG + chr1_3:13984584-13984603 None:intergenic 35.0%
CAGAATTTCCTAATAAGACC+TGG + chr1_3:13984586-13984605 None:intergenic 35.0%
AAAACATTGTTGCATACCCT+AGG + chr1_3:13984650-13984669 None:intergenic 35.0%
!!! CTACTGTTCAGTTATTTTGG+CGG + chr1_3:13984777-13984796 None:intergenic 35.0%
GCATTTGTAATAGATTGCCA+TGG + chr1_3:13984917-13984936 None:intergenic 35.0%
! TATTTAGACAATGGTGGAAG+TGG - chr1_3:13985001-13985020 Msa0105470:intron 35.0%
CGGTATATGTGTAGTATATG+TGG - chr1_3:13985175-13985194 Msa0105470:intron 35.0%
GGACAAGTAAATTACTCATC+TGG - chr1_3:13985196-13985215 Msa0105470:intron 35.0%
! TATGAGAAGACTTTTGAGCA+TGG - chr1_3:13985407-13985426 Msa0105470:intron 35.0%
TTCTCTTCAGAAATGAGGAA+TGG + chr1_3:13985501-13985520 None:intergenic 35.0%
GCTTGAGCATTAAGAAATAG+TGG + chr1_3:13985687-13985706 None:intergenic 35.0%
TATCTATCTCATCATGAGCA+AGG - chr1_3:13985727-13985746 Msa0105470:intron 35.0%
TAGTCGAATTAAGCGTATCA+TGG - chr1_3:13985805-13985824 Msa0105470:intron 35.0%
! CTTGCTATATGACAATCTGA+GGG + chr1_3:13986115-13986134 None:intergenic 35.0%
AAGGACAGTTGTAACTTCTA+AGG - chr1_3:13986132-13986151 Msa0105470:CDS 35.0%
TATTGTGAATTTGTCCCTAC+TGG - chr1_3:13986232-13986251 Msa0105470:intron 35.0%
TAAGTATTCAACGAGTTGCA+AGG - chr1_3:13986288-13986307 Msa0105470:CDS 35.0%
!! ATCCACTTTGGTGCCATTAA+TGG + chr1_3:13983199-13983218 None:intergenic 40.0%
GATAACTGGACTATCCACTT+TGG + chr1_3:13983211-13983230 None:intergenic 40.0%
! CAGTTATCCAGTCTGTTTTG+CGG - chr1_3:13983223-13983242 Msa0105470:three_prime_UTR 40.0%
AAGCGCTTGTTCAATGTTTC+TGG + chr1_3:13983460-13983479 None:intergenic 40.0%
AAACATTGAACAAGCGCTTC+AGG - chr1_3:13983461-13983480 Msa0105470:three_prime_UTR 40.0%
!!! GCGTAATAGCCTTTTTTCAG+TGG + chr1_3:13983591-13983610 None:intergenic 40.0%
AATGTCAGGAAAGAGTGATG+AGG - chr1_3:13983843-13983862 Msa0105470:intron 40.0%
! ACGCCATCAATTAGAAGTGT+CGG + chr1_3:13983943-13983962 None:intergenic 40.0%
TTGTGAGATTGTTTGGGAGA+AGG - chr1_3:13984207-13984226 Msa0105470:intron 40.0%
GCAATTGAACCATTACTCAG+AGG + chr1_3:13984703-13984722 None:intergenic 40.0%
GGTGAGTATTTAGACAATGG+TGG - chr1_3:13984995-13985014 Msa0105470:intron 40.0%
CACCAGATGCGAAAATTGTT+TGG + chr1_3:13985123-13985142 None:intergenic 40.0%
AGAGTGTACCAGAAAGAGTA+AGG + chr1_3:13985146-13985165 None:intergenic 40.0%
TGGTACACTCTCTTTCTCTT+CGG - chr1_3:13985155-13985174 Msa0105470:intron 40.0%
GCTAACTATTGAAGTCCTTC+CGG - chr1_3:13985382-13985401 Msa0105470:intron 40.0%
AAGTCTTCTCATATACCGGA+AGG + chr1_3:13985400-13985419 None:intergenic 40.0%
! TTTTCCTCTGTTTCGTACTC+CGG + chr1_3:13985850-13985869 None:intergenic 40.0%
AATCACTGATGAGCACTTCT+TGG - chr1_3:13985868-13985887 Msa0105470:intron 40.0%
! CCATGTTGGGGTATTCAAAA+TGG + chr1_3:13985900-13985919 None:intergenic 40.0%
! CCATTTTGAATACCCCAACA+TGG - chr1_3:13985897-13985916 Msa0105470:intron 40.0%
! TTTTGAATACCCCAACATGG+TGG - chr1_3:13985900-13985919 Msa0105470:intron 40.0%
! TGATCCTGAGGATTTTCGTA+CGG - chr1_3:13985925-13985944 Msa0105470:intron 40.0%
! CCTTGCTATATGACAATCTG+AGG + chr1_3:13986116-13986135 None:intergenic 40.0%
CCTCAGATTGTCATATAGCA+AGG - chr1_3:13986113-13986132 Msa0105470:CDS 40.0%
AGTATCATTCTAGCCTCAAG+TGG - chr1_3:13986173-13986192 Msa0105470:intron 40.0%
! GAGTTGCAAGGTTTTTCCTT+TGG - chr1_3:13986300-13986319 Msa0105470:CDS 40.0%
!! ATGGAATTGGATGGTGCCAT+TGG - chr1_3:13983171-13983190 Msa0105470:three_prime_UTR 45.0%
TGCCATTAATGGCACCAAAG+TGG - chr1_3:13983194-13983213 Msa0105470:three_prime_UTR 45.0%
CGCAAAACAGACTGGATAAC+TGG + chr1_3:13983225-13983244 None:intergenic 45.0%
TTTGAACGGCCCAGATTGAA+AGG - chr1_3:13983271-13983290 Msa0105470:three_prime_UTR 45.0%
TTGAACGGCCCAGATTGAAA+GGG - chr1_3:13983272-13983291 Msa0105470:three_prime_UTR 45.0%
CCAAAAAATGCAATCGCTGC+CGG - chr1_3:13983354-13983373 Msa0105470:three_prime_UTR 45.0%
! TTTGTTATACGAGGCCACAC+CGG + chr1_3:13983376-13983395 None:intergenic 45.0%
ATTGCAGCAGGAAATTACGC+TGG + chr1_3:13983563-13983582 None:intergenic 45.0%
!! TCTTTGAAGCCTCTTCGTGA+TGG - chr1_3:13983811-13983830 Msa0105470:intron 45.0%
!! GATGGTGGAAAAGGAATGTC+AGG - chr1_3:13983829-13983848 Msa0105470:intron 45.0%
! GCACCGACACTTCTAATTGA+TGG - chr1_3:13983937-13983956 Msa0105470:intron 45.0%
GTGTTGTGTTCGTGCTTCAT+AGG - chr1_3:13984066-13984085 Msa0105470:intron 45.0%
GATAAGCAGTTGAAGGTCCT+GGG + chr1_3:13984531-13984550 None:intergenic 45.0%
GCTAAGGGATAAGCAGTTGA+AGG + chr1_3:13984538-13984557 None:intergenic 45.0%
!! GCTCAACTGGTTTGAGCTAA+GGG + chr1_3:13984553-13984572 None:intergenic 45.0%
!! AGCTCAACTGGTTTGAGCTA+AGG + chr1_3:13984554-13984573 None:intergenic 45.0%
TCTCCAGTTCAACTTCTGGT+CGG + chr1_3:13984678-13984697 None:intergenic 45.0%
CTTCATCATCAGATCCACCA+GGG + chr1_3:13985084-13985103 None:intergenic 45.0%
TCTTCATCATCAGATCCACC+AGG + chr1_3:13985085-13985104 None:intergenic 45.0%
! CGCCAAACAATTTTCGCATC+TGG - chr1_3:13985118-13985137 Msa0105470:intron 45.0%
ATCTGGTGCCTTACTCTTTC+TGG - chr1_3:13985135-13985154 Msa0105470:intron 45.0%
TGATGAGCACTTCTTGGAGA+AGG - chr1_3:13985874-13985893 Msa0105470:intron 45.0%
AGGATCATACCACCATGTTG+GGG + chr1_3:13985912-13985931 None:intergenic 45.0%
CAGGATCATACCACCATGTT+GGG + chr1_3:13985913-13985932 None:intergenic 45.0%
TCAGGATCATACCACCATGT+TGG + chr1_3:13985914-13985933 None:intergenic 45.0%
AACATGGTGGTATGATCCTG+AGG - chr1_3:13985913-13985932 Msa0105470:intron 45.0%
GAGTTATTCGTCTGCATCTG+AGG + chr1_3:13985969-13985988 None:intergenic 45.0%
GGCACAACAAATGCAAGGAA+AGG + chr1_3:13986094-13986113 None:intergenic 45.0%
TCCACTAGACAAACCACTTG+AGG + chr1_3:13986189-13986208 None:intergenic 45.0%
CTCGCTCAAAGAAACCAGTA+GGG + chr1_3:13986249-13986268 None:intergenic 45.0%
!! GGTGCCATTGGTGCCATTAA+TGG - chr1_3:13983183-13983202 Msa0105470:three_prime_UTR 50.0%
!! GGTGCCATTAATGGCACCAA+TGG + chr1_3:13983192-13983211 None:intergenic 50.0%
CACACATCCGCAAAACAGAC+TGG + chr1_3:13983233-13983252 None:intergenic 50.0%
! TTTTGCGGATGTGTGACACG+TGG - chr1_3:13983238-13983257 Msa0105470:three_prime_UTR 50.0%
TTTGCGGATGTGTGACACGT+GGG - chr1_3:13983239-13983258 Msa0105470:three_prime_UTR 50.0%
!!! CCGGCAGCGATTGCATTTTT+TGG + chr1_3:13983357-13983376 None:intergenic 50.0%
TCGCACTGAACTCATCTCAC+CGG - chr1_3:13983406-13983425 Msa0105470:three_prime_UTR 50.0%
!! TTCAATGTTTCTGGCGCCGA+CGG + chr1_3:13983451-13983470 None:intergenic 50.0%
TCAAGCGCGTTGCATGATAG+GGG + chr1_3:13983530-13983549 None:intergenic 50.0%
CTCAAGCGCGTTGCATGATA+GGG + chr1_3:13983531-13983550 None:intergenic 50.0%
TCAGTGGCGATTATTGCAGC+AGG + chr1_3:13983575-13983594 None:intergenic 50.0%
TTGAAGCCTCTTCGTGATGG+TGG - chr1_3:13983814-13983833 Msa0105470:intron 50.0%
! CCTTTTCCACCATCACGAAG+AGG + chr1_3:13983823-13983842 None:intergenic 50.0%
!! CCTCTTCGTGATGGTGGAAA+AGG - chr1_3:13983820-13983839 Msa0105470:intron 50.0%
!! TAGAAGTGTCGGTGCTACAG+AGG + chr1_3:13983932-13983951 None:intergenic 50.0%
GGATAAGCAGTTGAAGGTCC+TGG + chr1_3:13984532-13984551 None:intergenic 50.0%
CCTAATAAGACCTGGGGGTT+GGG + chr1_3:13984578-13984597 None:intergenic 50.0%
TCCTAATAAGACCTGGGGGT+TGG + chr1_3:13984579-13984598 None:intergenic 50.0%
GAACCGACCAGAAGTTGAAC+TGG - chr1_3:13984672-13984691 Msa0105470:intron 50.0%
GGTCTCTCCAGTTCAACTTC+TGG + chr1_3:13984682-13984701 None:intergenic 50.0%
CTGGAGAGACCTCTGAGTAA+TGG - chr1_3:13984691-13984710 Msa0105470:intron 50.0%
TGTACCGGAGTACGAAACAG+AGG - chr1_3:13985843-13985862 Msa0105470:intron 50.0%
ACTGCCGTACGAAAATCCTC+AGG + chr1_3:13985932-13985951 None:intergenic 50.0%
! CTGAGGGCACAACAAATGCA+AGG + chr1_3:13986099-13986118 None:intergenic 50.0%
! GCCTCAAGTGGTTTGTCTAG+TGG - chr1_3:13986185-13986204 Msa0105470:intron 50.0%
CCTCGCTCAAAGAAACCAGT+AGG + chr1_3:13986250-13986269 None:intergenic 50.0%
!! CCTACTGGTTTCTTTGAGCG+AGG - chr1_3:13986247-13986266 Msa0105470:intron 50.0%
AAATGCAATCGCTGCCGGTG+TGG - chr1_3:13983359-13983378 Msa0105470:three_prime_UTR 55.0%
!! TCAATGTTTCTGGCGCCGAC+GGG + chr1_3:13983450-13983469 None:intergenic 55.0%
GCTCAAGCGCGTTGCATGAT+AGG + chr1_3:13983532-13983551 None:intergenic 55.0%
!! CCCAACCCCCAGGTCTTATT+AGG - chr1_3:13984575-13984594 Msa0105470:CDS 55.0%
GGAAGTGGAGTAAAGCCACC+AGG - chr1_3:13985016-13985035 Msa0105470:intron 55.0%
AGTGGAGTAAAGCCACCAGG+AGG - chr1_3:13985019-13985038 Msa0105470:intron 55.0%
TCAGATCCACCAGGGCCATT+GGG + chr1_3:13985076-13985095 None:intergenic 55.0%
ATCAGATCCACCAGGGCCAT+TGG + chr1_3:13985077-13985096 None:intergenic 55.0%
CTGGGCTCAAACCAGGACGA+AGG + chr1_3:13984513-13984532 None:intergenic 60.0%
! CTTCGTCCTGGTTTGAGCCC+AGG - chr1_3:13984511-13984530 Msa0105470:CDS 60.0%
GAAGGTCCTGGGCTCAAACC+AGG + chr1_3:13984520-13984539 None:intergenic 60.0%
TGGGGGTTGGGTAGCTCAAC+TGG + chr1_3:13984566-13984585 None:intergenic 60.0%
AGTTGAGCTACCCAACCCCC+AGG - chr1_3:13984565-13984584 Msa0105470:CDS 60.0%
GGAGTAAAGCCACCAGGAGG+AGG - chr1_3:13985022-13985041 Msa0105470:intron 60.0%
GTAAAGCCACCAGGAGGAGG+AGG - chr1_3:13985025-13985044 Msa0105470:intron 60.0%
CAGGAGGAGGAGGAGAAGGT+GGG - chr1_3:13985035-13985054 Msa0105470:intron 60.0%
AGGAGGAGGAGGAGAAGGTG+GGG - chr1_3:13985036-13985055 Msa0105470:intron 60.0%
ATCTCACCGGAAACCGCCGC+CGG - chr1_3:13983419-13983438 Msa0105470:three_prime_UTR 65.0%
GAAACTCCGGCGGCGGTTTC+CGG + chr1_3:13983428-13983447 None:intergenic 65.0%
CCTTCTCCTCCTCCTCCTGG+TGG + chr1_3:13985034-13985053 None:intergenic 65.0%
CCACCAGGAGGAGGAGGAGA+AGG - chr1_3:13985031-13985050 Msa0105470:intron 65.0%
CCACCTTCTCCTCCTCCTCC+TGG + chr1_3:13985037-13985056 None:intergenic 65.0%
CCAGGAGGAGGAGGAGAAGG+TGG - chr1_3:13985034-13985053 Msa0105470:intron 65.0%
GGAGGAGGAGGAGAAGGTGG+GGG - chr1_3:13985037-13985056 Msa0105470:intron 65.0%
!! GGTGGTGGTGGCAGTCCCAA+TGG - chr1_3:13985058-13985077 Msa0105470:intron 65.0%
!! CTGGCGCCGACGGGAAACTC+CGG + chr1_3:13983441-13983460 None:intergenic 70.0%
GGAGGAGGAGAAGGTGGGGG+TGG - chr1_3:13985040-13985059 Msa0105470:intron 70.0%
GGAGGAGAAGGTGGGGGTGG+TGG - chr1_3:13985043-13985062 Msa0105470:intron 70.0%
! GGAGAAGGTGGGGGTGGTGG+TGG - chr1_3:13985046-13985065 Msa0105470:intron 70.0%
GGTGGCAGTCCCAATGGCCC+TGG - chr1_3:13985064-13985083 Msa0105470:intron 70.0%
GGCAGTCCCAATGGCCCTGG+TGG - chr1_3:13985067-13985086 Msa0105470:intron 70.0%
CCGACGGGAAACTCCGGCGG+CGG + chr1_3:13983435-13983454 None:intergenic 75.0%
CCGCCGCCGGAGTTTCCCGT+CGG - chr1_3:13983432-13983451 Msa0105470:three_prime_UTR 75.0%
! GCGCCGACGGGAAACTCCGG+CGG + chr1_3:13983438-13983457 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 13983171 13986332 13983171 ID=Msa0105470;Name=Msa0105470
chr1_3 mRNA 13983171 13986332 13983171 ID=Msa0105470-mRNA-1;Parent=Msa0105470;Name=Msa0105470-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0.5|0.4|0.8|1|1|5|347|279
chr1_3 exon 13983171 13983778 13983171 ID=Msa0105470-mRNA-1:exon:5480;Parent=Msa0105470-mRNA-1
chr1_3 exon 13984347 13984595 13984347 ID=Msa0105470-mRNA-1:exon:5479;Parent=Msa0105470-mRNA-1
chr1_3 exon 13985639 13985737 13985639 ID=Msa0105470-mRNA-1:exon:5478;Parent=Msa0105470-mRNA-1
chr1_3 exon 13986021 13986189 13986021 ID=Msa0105470-mRNA-1:exon:5477;Parent=Msa0105470-mRNA-1
chr1_3 exon 13986271 13986332 13986271 ID=Msa0105470-mRNA-1:exon:5476;Parent=Msa0105470-mRNA-1
chr1_3 CDS 13986271 13986332 13986271 ID=Msa0105470-mRNA-1:cds;Parent=Msa0105470-mRNA-1
chr1_3 CDS 13986021 13986189 13986021 ID=Msa0105470-mRNA-1:cds;Parent=Msa0105470-mRNA-1
chr1_3 CDS 13985639 13985737 13985639 ID=Msa0105470-mRNA-1:cds;Parent=Msa0105470-mRNA-1
chr1_3 CDS 13984347 13984595 13984347 ID=Msa0105470-mRNA-1:cds;Parent=Msa0105470-mRNA-1
chr1_3 CDS 13983518 13983778 13983518 ID=Msa0105470-mRNA-1:cds;Parent=Msa0105470-mRNA-1
chr1_3 three_prime_UTR 13983171 13983517 13983171 ID=Msa0105470-mRNA-1:three_prime_utr;Parent=Msa0105470-mRNA-1
Gene Sequence

>Msa0105470

ATGGAATTGGATGGTGCCATTGGTGCCATTAATGGCACCAAAGTGGATAGTCCAGTTATCCAATCTGAACTCTGCAAGCCTAATCCATTTCCTTTTGAAAAGCCAAAAAATGCAATCGCTGCCGGTGTGGCCTCGTATAACAAACAGCTTACAGTCGCACTGAACTCATCTCACCGGAAACCGCCGCCGGAGTTTCCCGTCGGCGCCAGAAACATTGAACAAGCGCTTCAGATTCGGAATATTAGCATAAGAGATAAACCGTTATTTGGATTGAAATCTTTGAAGCCTCTTCGTGATGGTGGAAAAGGAATGTCAGGAAAGAGTGATGAGAATTCCCCATGGCAATCTATTACAAATGCAATGGACAAATTTAAGGGACAATCAATAGAAGAAGTATTACGGAAGCAAATTCAAAAAGGTGAGTATTTAGACAATGGTGGAAGTGGAGTAAAGCCACCAGGAGGAGGAGGAGAAGGTGGGGGTGGTGGTGGCAGTCCCAATGGCCCTGGTGGATCTGATGATGAAGAAAGCTATGACACTCGCCAAACAATTTTCGCATCTGGTGCCTTACTCTTTCTGTATATCTATCTCATCATGAGCAAGGAGCTTCTAAGCACAATTTTCGACTTTTTAAGGTTTATAGTTGGCTTAGGTCAGACTAGTCGAATTAAGCGTATCATGGTAAAATTGGGAAAATTGTACCGGAGTACGAAACAGAGGAAAATCACTGATGAGCACTTCTTGGAGAAGGCCATTTTGAATACCCCAACATGGTGGTATGATCCTGAGGATTTTCGTACGGCAGTTAAGAATTACTATGCCTCAGATGCAGACGAATAA
Protein sequence

>Msa0105470

MELDGAIGAINGTKVDSPVIQSELCKPNPFPFEKPKNAIAAGVASYNKQLTVALNSSHRKPPPEFPVGARNIEQALQIRNISIRDKPLFGLKSLKPLRDGGKGMSGKSDENSPWQSITNAMDKFKGQSIEEVLRKQIQKGEYLDNGGSGVKPPGGGGEGGGGGGSPNGPGGSDDEESYDTRQTIFASGALLFLYIYLIMSKELLSTIFDFLRFIVGLGQTSRIKRIMVKLGKLYRSTKQRKITDEHFLEKAILNTPTWWYDPEDFRTAVKNYYASDADE*