AlfalfaGEDB | Msa1462700

M. sativa cultivar zhongmu-4 / Msa1462700

KEGG: K01602
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
PFAM: PF12338.11

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1462700	WP_077269212.1	89.831	177	0	1	1	159	1	177	8.82e-111	322

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1462700	sp\|O65194\|RBS_MEDSA	86.111	180	4	2	1	159	1	180	4.64e-109	311

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1462700	G7KMR3	84.181	177	10	1	1	159	1	177	7.44e-104	304

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa1462700	0.838915	2.165701e-57	-8.615850e-47
Msa0000800	Msa1462700	-0.818608	1.793763e-52	-8.615850e-47
Msa0001040	Msa1462700	0.819167	1.338691e-52	-8.615850e-47
Msa0001420	Msa1462700	0.803090	4.114623e-49	-8.615850e-47
Msa0001510	Msa1462700	0.937766	2.028459e-98	-8.615850e-47
Msa0001540	Msa1462700	0.864655	1.040070e-64	-8.615850e-47
Msa0001550	Msa1462700	0.834957	2.219132e-56	-8.615850e-47
Msa0002060	Msa1462700	0.838868	2.226893e-57	-8.615850e-47
Msa0002110	Msa1462700	0.891240	4.760072e-74	-8.615850e-47
Msa0002690	Msa1462700	0.948838	4.251490e-107	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1462700	MtrunA17_Chr6g0457461	84.181	177	10	1	1	159	1	177	1.43e-107	304
Msa1462700	MtrunA17_Chr6g0457471	82.222	180	11	2	1	159	1	180	9.94e-105	297
Msa1462700	MtrunA17_Chr7g0215381	73.256	172	24	3	9	159	11	181	2.70e-88	255
Msa1462700	MtrunA17_Chr7g0215351	73.837	172	23	3	9	159	11	181	3.11e-88	255
Msa1462700	MtrunA17_Chr7g0215421	73.256	172	24	3	9	159	11	181	5.74e-88	254
Msa1462700	MtrunA17_Chr7g0215361	73.684	171	23	3	10	159	12	181	7.31e-88	254
Msa1462700	MtrunA17_Chr7g0215431	72.515	171	25	3	10	159	12	181	1.25e-86	251
Msa1462700	MtrunA17_Chr4g0072241	45.161	155	66	2	23	158	15	169	4.20e-47	150

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1462700	AT1G67090.1	66.279	172	40	1	6	159	7	178	8.13e-83	242
Msa1462700	AT5G38410.1	65.909	176	41	2	2	159	4	178	9.17e-83	242
Msa1462700	AT5G38430.1	65.909	176	41	2	2	159	4	178	1.30e-82	241
Msa1462700	AT5G38430.2	65.909	176	41	2	2	159	4	178	1.30e-82	241
Msa1462700	AT5G38410.3	64.088	181	41	2	2	159	4	183	5.67e-82	240
Msa1462700	AT5G38420.1	64.773	176	43	2	2	159	4	178	4.43e-81	237
Msa1462700	AT5G38410.2	66.272	169	45	2	2	159	4	171	1.47e-80	236
Msa1462700	AT1G67090.2	67.857	84	27	0	6	89	7	90	9.62e-37	124

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Find 77 sgRNAs with CRISPR-Local

Find 269 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAAGTAGTAATAAAGGG+TGG	0.668091	tig0042521:+1365	Msa1462700:intergenic
AACGGTGGTAACAGCGGCGG+AGG	0.513043	tig0042521:+2610	Msa1462700:intergenic
AACTCATTCATCAGAATCAT+TGG	0.341056	tig0042521:-1466	Msa1462700:CDS
AACTTCTTCTTGCCAACTGG+TGG	0.532490	tig0042521:+2191	Msa1462700:intergenic
AACTTGGTTGCTCCGTTCAC+CGG	0.448528	tig0042521:-2572	Msa1462700:CDS
AATGGAGGTAATGTCATTGT+TGG	0.536427	tig0042521:+2511	Msa1462700:intergenic
AATGTCATTGTTGGTCTTCT+TGG	0.291082	tig0042521:+2520	Msa1462700:intergenic
AATTGTTCCTCAGTGAGGGG+TGG	0.640814	tig0042521:+2149	Msa1462700:intergenic
ACAAACAAAGTAGTAATAAA+GGG	0.474757	tig0042521:+1362	Msa1462700:intergenic
ACCAGCTGAGGATTTGAGAC+CGG	0.573385	tig0042521:+2553	Msa1462700:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCTAATGGTATGGAATTGAA+AGG	+	tig0042521:1119-1138	Msa1462700:intergenic	30.0%
CTAAGAAGGAAAAGGAAAAA+TGG	-	tig0042521:1226-1245	Msa1462700:three_prime_UTR	30.0%
AAAGGAAAAAAAGGTTGCAA+TGG	+	tig0042521:1822-1841	Msa1462700:intergenic	30.0%
TGATTCTGATGAATGAGTTT+GGG	+	tig0042521:2401-2420	Msa1462700:intergenic	30.0%
ATGATTCTGATGAATGAGTT+TGG	+	tig0042521:2402-2421	Msa1462700:intergenic	30.0%
AACTCATTCATCAGAATCAT+TGG	-	tig0042521:2402-2421	Msa1462700:intron	30.0%
TATCAAACTTAGTAGGTTTC+TGG	+	tig0042521:2477-2496	Msa1462700:intergenic	30.0%
GTGCAAATATCAAACTTAGT+AGG	+	tig0042521:2484-2503	Msa1462700:intergenic	30.0%
AACAAAGTAGTAATAAAGGG+TGG	+	tig0042521:2506-2525	Msa1462700:intergenic	30.0%
GGGAAATATAGTTCTTACAA+CGG	+	tig0042521:2538-2557	Msa1462700:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0042521	gene	1019	2871	1019	ID=Msa1462700;Name=Msa1462700
tig0042521	mRNA	1019	2871	1019	ID=Msa1462700-mRNA-1;Parent=Msa1462700;Name=Msa1462700-mRNA-1;_AED=0.05;_eAED=0.05;_QI=226\|0.5\|0.66\|1\|1\|1\|3\|383\|159
tig0042521	exon	1019	1632	1019	ID=Msa1462700-mRNA-1:exon:23773;Parent=Msa1462700-mRNA-1
tig0042521	exon	2139	2219	2139	ID=Msa1462700-mRNA-1:exon:23772;Parent=Msa1462700-mRNA-1
tig0042521	exon	2478	2871	2478	ID=Msa1462700-mRNA-1:exon:23771;Parent=Msa1462700-mRNA-1
tig0042521	five_prime_UTR	2646	2871	2646	ID=Msa1462700-mRNA-1:five_prime_utr;Parent=Msa1462700-mRNA-1
tig0042521	CDS	2478	2645	2478	ID=Msa1462700-mRNA-1:cds;Parent=Msa1462700-mRNA-1
tig0042521	CDS	2139	2219	2139	ID=Msa1462700-mRNA-1:cds;Parent=Msa1462700-mRNA-1
tig0042521	CDS	1402	1632	1402	ID=Msa1462700-mRNA-1:cds;Parent=Msa1462700-mRNA-1
tig0042521	three_prime_UTR	1019	1401	1019	ID=Msa1462700-mRNA-1:three_prime_utr;Parent=Msa1462700-mRNA-1

Gene Sequence

>Msa1462700

ATGGCTTTGATCTCCTCCGCCGCTGTTACCACCGTTAACCGCGTCTCCGCTAACTTGGTTGCTCCGTTCACCGGTCTCAAATCCTCAGCTGGTTTCCCAGTCACCAAGAAGACCAACAATGACATTACCTCCATTGCAAGCAATGGTGGAAGAGTGAACTGTATGCAGGTGTGGCCACCAGTTGGCAAGAAGAAGTTTGAGACACTTTCATACCTTCCACCCCTCACTGAGGAACAATTGGCTAAGGAAAAAGGATTTGTCTACCGTGAGAACCACCGTTCACCAGGATACTATGACGGACGTTACTGGACAATGTGGAGGTTGCCTTTGTTCGGAGCCACTGATTCTTCTCAAGTGTTGAAGGAGCTTGCTGACTGTAAAGCTGAATACCCAAACTCATTCATCAGAATCATTGGATTTGACAATGTTCGTCAAGTGCAGTGCATTAGTTTCATTGCACACACACCAGAAACCTACTAA

Protein sequence

>Msa1462700

MALISSAAVTTVNRVSANLVAPFTGLKSSAGFPVTKKTNNDITSIASNGGRVNCMQVWPPVGKKKFETLSYLPPLTEEQLAKEKGFVYRENHRSPGYYDGRYWTMWRLPLFGATDSSQVLKELADCKAEYPNSFIRIIGFDNVRQVQCISFIAHTPETY*