AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0745270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0745270 MtrunA17_Chr5g0448421 98.917 277 3 0 1 277 1 277 0.0 554
Msa0745270 MtrunA17_Chr5g0448411 95.307 277 11 1 1 277 1 275 0.0 531
Msa0745270 MtrunA17_Chr3g0133901 47.436 234 101 9 47 274 45 262 5.24e-61 194
Msa0745270 MtrunA17_Chr8g0345631 42.500 240 112 7 37 274 50 265 6.35e-52 171
Msa0745270 MtrunA17_Chr3g0092091 40.727 275 127 10 9 274 2 249 1.57e-48 161
Msa0745270 MtrunA17_Chr2g0301081 43.111 225 101 7 56 274 61 264 2.28e-48 161
Msa0745270 MtrunA17_Chr4g0051941 37.868 272 131 11 10 269 9 254 1.37e-38 136
Msa0745270 MtrunA17_Chr5g0447531 38.542 288 123 15 1 269 1 253 7.63e-38 134
Msa0745270 MtrunA17_Chr6g0453141 36.466 266 134 9 14 269 13 253 3.45e-37 132
Msa0745270 MtrunA17_Chr6g0453151 41.014 217 93 9 63 269 129 320 5.32e-37 134
Msa0745270 MtrunA17_Chr6g0453181 40.553 217 94 9 63 269 62 253 6.66e-37 132
Msa0745270 MtrunA17_Chr2g0318521 41.475 217 94 8 63 269 59 252 8.47e-37 131
Msa0745270 MtrunA17_Chr6g0462031 41.395 215 93 8 60 270 49 234 1.50e-35 127
Msa0745270 MtrunA17_Chr6g0453161 36.559 279 141 10 1 269 268 520 2.37e-35 132
Msa0745270 MtrunA17_Chr6g0453161 41.014 217 93 9 63 269 64 255 1.12e-34 130
Msa0745270 MtrunA17_Chr6g0476641 38.211 246 114 10 41 274 60 279 1.16e-34 126
Msa0745270 MtrunA17_Chr1g0157481 33.574 277 138 10 26 277 78 333 3.52e-32 121
Msa0745270 MtrunA17_Chr6g0464511 34.146 205 86 6 65 269 45 200 3.60e-26 102
Msa0745270 MtrunA17_Chr2g0279001 35.567 194 91 8 69 247 75 249 6.60e-25 100
Msa0745270 MtrunA17_Chr4g0006181 30.418 263 112 6 55 274 110 344 1.13e-24 101
Msa0745270 MtrunA17_Chr6g0453171 58.108 74 30 1 196 269 14 86 6.96e-23 90.1
Msa0745270 MtrunA17_Chr3g0113311 30.126 239 96 6 59 254 59 269 3.73e-18 82.0
Msa0745270 MtrunA17_Chr2g0296081 53.333 75 30 2 200 274 32 101 1.95e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0745270 AT1G61520.3 85.921 277 35 3 1 277 1 273 1.40e-167 465
Msa0745270 AT1G61520.1 85.921 277 35 3 1 277 1 273 1.40e-167 465
Msa0745270 AT1G61520.2 89.545 220 18 1 58 277 4 218 1.36e-144 404
Msa0745270 AT1G45474.2 42.188 256 126 9 23 274 14 251 1.07e-55 180
Msa0745270 AT1G45474.1 42.188 256 126 9 23 274 14 251 1.07e-55 180
Msa0745270 AT3G61470.1 42.500 240 115 7 41 274 32 254 2.97e-50 166
Msa0745270 AT3G47470.1 43.651 252 97 10 30 274 37 250 2.62e-48 161
Msa0745270 AT1G19150.1 38.168 262 138 7 18 277 31 270 3.68e-46 156
Msa0745270 AT3G27690.1 38.028 284 131 14 1 269 1 254 7.72e-39 137
Msa0745270 AT3G27690.2 38.028 284 131 14 1 269 33 286 1.38e-38 137
Msa0745270 AT2G05100.1 38.163 283 131 14 1 269 1 253 1.90e-38 136
Msa0745270 AT2G05070.1 37.809 283 132 14 1 269 1 253 1.37e-37 134
Msa0745270 AT5G54270.1 41.935 217 93 8 63 269 60 253 3.85e-37 132
Msa0745270 AT1G29930.1 38.745 271 131 9 10 269 9 255 6.38e-37 132
Msa0745270 AT1G29920.1 38.745 271 131 9 10 269 9 255 1.66e-36 131
Msa0745270 AT1G29910.1 38.745 271 131 9 10 269 9 255 1.66e-36 131
Msa0745270 AT2G34420.1 37.276 279 139 10 1 269 1 253 6.07e-36 129
Msa0745270 AT2G34430.1 37.903 248 114 10 38 269 31 254 2.49e-35 128
Msa0745270 AT3G54890.1 40.465 215 95 8 60 270 48 233 4.20e-35 127
Msa0745270 AT4G10340.1 39.184 245 111 10 42 274 52 270 3.64e-34 125
Msa0745270 AT1G76570.1 31.271 291 157 10 2 275 62 326 4.51e-30 115
Msa0745270 AT3G08940.2 30.935 278 121 6 40 274 35 284 8.15e-28 108
Msa0745270 AT2G05100.2 35.316 269 130 14 1 255 1 239 2.30e-27 108
Msa0745270 AT5G01530.1 30.038 263 113 6 55 274 53 287 1.39e-24 100
Msa0745270 AT1G15820.1 33.735 249 114 13 24 247 24 246 3.26e-24 98.6
Msa0745270 AT3G54890.4 37.079 178 80 7 60 234 48 196 1.28e-21 90.5
Msa0745270 AT2G40100.1 30.400 250 102 8 52 258 51 271 8.85e-17 78.6
Msa0745270 AT3G54890.2 31.532 222 71 9 60 270 48 199 1.61e-16 76.6
Msa0745270 AT5G28450.1 43.137 102 55 2 174 274 71 170 2.06e-16 75.5

Find 118 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGCTTTGCCGAGAATTTC+AGG 0.135748 5_1:-66483610 None:intergenic
AATAAATAAAACTTGTAATT+TGG 0.148502 5_1:+66484387 Msa0745270:three_prime_UTR
GTTAGCTATGTTGGCAATAT+TGG 0.175999 5_1:+66483951 Msa0745270:CDS
TTTGTTCAAGCTCTTGTTAC+AGG 0.246233 5_1:+66483979 Msa0745270:CDS
ATCATCACTTGCAGCCTTCC+TGG 0.261258 5_1:+66483454 Msa0745270:intron
ATATTTAACCCTCTTGGTTT+TGG 0.269352 5_1:+66483874 Msa0745270:CDS
ATGGGAAAACAATACTTCTT+AGG 0.280187 5_1:+66483799 Msa0745270:CDS
CCAGATGATCTAAGAGGTTT+TGG 0.293540 5_1:-66484014 None:intergenic
TTGCTCTTGTTGGTTGTTGT+AGG 0.321314 5_1:+66484118 Msa0745270:three_prime_UTR
TCATATCTTCCTGATCCATT+TGG 0.346145 5_1:+66482062 Msa0745270:five_prime_UTR
TTAGCTATGTTGGCAATATT+GGG 0.350553 5_1:+66483952 Msa0745270:CDS
TATATAATACATTCATTTAG+TGG 0.359289 5_1:-66484077 None:intergenic
AAAGCAAAGTCTTTCTTACT+TGG 0.368436 5_1:+66482585 Msa0745270:CDS
AGATCACTCCCATCTTCAAT+AGG 0.372428 5_1:+66482259 Msa0745270:CDS
TTAGGACTTGAGAAAGGTCT+TGG 0.373705 5_1:+66483817 Msa0745270:CDS
TGCAGCCTTCCTGGTGACTT+TGG 0.374400 5_1:+66483463 Msa0745270:intron
TTCTTCAAGAATGTAGTAGA+TGG 0.375775 5_1:-66482121 None:intergenic
CCAAAACCTCTTAGATCATC+TGG 0.385529 5_1:+66484014 Msa0745270:CDS
AATGAATCCACCAGTTCCTT+CGG 0.395198 5_1:-66483512 None:intergenic
TGCATCCACATGGCTACATT+TGG 0.400944 5_1:+66482030 Msa0745270:five_prime_UTR
GAGAAAGGTCTTGGAGGTTC+CGG 0.403381 5_1:+66483826 Msa0745270:CDS
GGACCTATATTTAACCCTCT+TGG 0.406735 5_1:+66483868 Msa0745270:CDS
GAATGGAAGGTTAGCTATGT+TGG 0.408440 5_1:+66483942 Msa0745270:CDS
CAAGCTCTTGTTACAGGAGT+TGG 0.415706 5_1:+66483985 Msa0745270:CDS
CTGCAGGAACATACAACTAC+TGG 0.419959 5_1:+66483686 Msa0745270:CDS
TGTGATGGAAATGGCATTCA+TGG 0.422186 5_1:+66483729 Msa0745270:CDS
AGCCAGGATTGTACCAATCT+TGG 0.425513 5_1:-66483774 None:intergenic
ATACACGTAATATGATGAAT+TGG 0.425683 5_1:-66481926 None:intergenic
CAACGGCCGCTTCGCAATGT+TGG 0.427513 5_1:+66483567 Msa0745270:CDS
ATGAATCCACCAGTTCCTTC+GGG 0.429905 5_1:-66483511 None:intergenic
GTAATATGATGAATTGGATA+AGG 0.430261 5_1:-66481920 None:intergenic
AACTATAAGCCAAATGGATC+AGG 0.432629 5_1:-66482071 None:intergenic
AACGGCCGCTTCGCAATGTT+GGG 0.433209 5_1:+66483568 Msa0745270:CDS
CTAGAAGACCCTATTGAAGA+TGG 0.437182 5_1:-66482268 None:intergenic
CACACTCTTTGTGATGGAAA+TGG 0.442080 5_1:+66483720 Msa0745270:CDS
GTCAAGATGTTGTTGTTGAC+AGG 0.444048 5_1:-66484042 None:intergenic
CAAAGTCTTTCTTACTTGGA+TGG 0.445116 5_1:+66482589 Msa0745270:CDS
TCAATAGGGTCTTCTAGAAA+AGG 0.446420 5_1:+66482274 Msa0745270:CDS
AAACCAAGAGGGTTAAATAT+AGG 0.452200 5_1:-66483871 None:intergenic
TAATATGATGAATTGGATAA+GGG 0.458916 5_1:-66481919 None:intergenic
TGAAGTAACTATAAGCCAAA+TGG 0.461090 5_1:-66482077 None:intergenic
GTCTTTCTTACTTGGATGGA+AGG 0.462256 5_1:+66482593 Msa0745270:CDS
AAGACAAGAGAGTGAAGAAA+AGG 0.465124 5_1:-66484170 None:intergenic
TTCGCAATGTTGGGAGCAGC+CGG 0.465502 5_1:+66483577 Msa0745270:CDS
AACACAGAAGATTCCAAGAT+TGG 0.467039 5_1:+66483761 Msa0745270:CDS
CTTTCAGATCCCGAAGGAAC+TGG 0.478080 5_1:+66483502 Msa0745270:CDS
TCGCCATATGCTAACCATCT+TGG 0.478129 5_1:-66483538 None:intergenic
AAGCTGAAGGAAGTTAAGAA+TGG 0.479661 5_1:+66483925 Msa0745270:CDS
AGTGAGAGATAGACCTTGGC+AGG 0.481039 5_1:-66482328 None:intergenic
GCTATAGCACCTGAAATTCT+CGG 0.489754 5_1:+66483601 Msa0745270:CDS
AGCTTCTTCTCCTCCTGCCA+AGG 0.490862 5_1:+66482315 Msa0745270:CDS
TGTACGTGTCATTTCTTACA+AGG 0.494586 5_1:-66484225 None:intergenic
CCTGAAATTCTCGGCAAAGC+AGG 0.501852 5_1:+66483610 Msa0745270:CDS
TTCCCTCCTTTGTTACCTCT+AGG 0.504632 5_1:-66482157 None:intergenic
CATTTAGTGGAACTTGAGAC+TGG 0.504734 5_1:-66484064 None:intergenic
GCACTCCCTTGGTTCCAAAC+CGG 0.505888 5_1:+66483652 Msa0745270:CDS
TTGGTACAATCCTGGCTCCA+TGG 0.508162 5_1:+66483780 Msa0745270:CDS
GTGGATTCATTGAGCCAAGA+TGG 0.509728 5_1:+66483524 Msa0745270:CDS
GATCACTCCCATCTTCAATA+GGG 0.512694 5_1:+66482260 Msa0745270:CDS
GAGCCAAGATGGTTAGCATA+TGG 0.514212 5_1:+66483535 Msa0745270:CDS
GAGGCTGTGAGACAGAGTCT+TGG 0.514771 5_1:+66482232 Msa0745270:CDS
GGACTTGAGAAAGGTCTTGG+AGG 0.517064 5_1:+66483820 Msa0745270:CDS
TAAAGATGAGAAATCATTGA+AGG 0.521323 5_1:+66483897 Msa0745270:CDS
ATAGGTCCACCAGGGTAAGC+TGG 0.522001 5_1:-66483853 None:intergenic
AAAAGGTTCCTTTGTTGTCA+AGG 0.526175 5_1:+66482291 Msa0745270:CDS
TGAAGGAAGTTAAGAATGGA+AGG 0.527760 5_1:+66483929 Msa0745270:CDS
ACAAGAGAGTGAAGAAAAGG+TGG 0.530505 5_1:-66484167 None:intergenic
CAGTAGTTGTATGTTCCTGC+AGG 0.533860 5_1:-66483685 None:intergenic
TTCCAAGATTGGTACAATCC+TGG 0.536164 5_1:+66483772 Msa0745270:CDS
AATTTCAGGTGCTATAGCTC+CGG 0.537587 5_1:-66483596 None:intergenic
CAAGGGAGTGCTGTTTCCGC+GGG 0.537601 5_1:-66483640 None:intergenic
ATCACGCCGGTTTGGAACCA+AGG 0.543476 5_1:-66483658 None:intergenic
ACCAGGGTAAGCTGGTTCGC+CGG 0.543633 5_1:-66483845 None:intergenic
TAGAAGACCCTATTGAAGAT+GGG 0.548284 5_1:-66482267 None:intergenic
CCAAGGGAGTGCTGTTTCCG+CGG 0.549385 5_1:-66483641 None:intergenic
TACTTCTTAGGACTTGAGAA+AGG 0.549404 5_1:+66483811 Msa0745270:CDS
TTCCTAGAGGTAACAAAGGA+GGG 0.554204 5_1:+66482155 Msa0745270:five_prime_UTR
TGTGAGCATGTTTCATGATG+TGG 0.565203 5_1:+66484202 Msa0745270:three_prime_UTR
ATTACGTGTATAAACATGGT+TGG 0.565397 5_1:+66481938 Msa0745270:five_prime_UTR
GACTTTGGATTTGACCCTCT+AGG 0.569949 5_1:+66483478 Msa0745270:CDS
TAGTTGTATGTTCCTGCAGG+TGG 0.570487 5_1:-66483682 None:intergenic
ACCGGCGTGATTCCACCTGC+AGG 0.575662 5_1:+66483670 Msa0745270:CDS
TGGTACAATCCTGGCTCCAT+GGG 0.575675 5_1:+66483781 Msa0745270:CDS
GGGTTAAATATAGGTCCACC+AGG 0.578557 5_1:-66483862 None:intergenic
TAACTACACACTCTTTGTGA+TGG 0.580645 5_1:+66483714 Msa0745270:CDS
GTGATGGAAATGGCATTCAT+GGG 0.581162 5_1:+66483730 Msa0745270:CDS
ATTGAAGGAGTTAAAGCTGA+AGG 0.587176 5_1:+66483912 Msa0745270:CDS
CATCTTTACCAAAACCAAGA+GGG 0.593825 5_1:-66483882 None:intergenic
TCATATTACGTGTATAAACA+TGG 0.603916 5_1:+66481934 Msa0745270:five_prime_UTR
CTTGTTCCTAGAGGTAACAA+AGG 0.604243 5_1:+66482151 Msa0745270:five_prime_UTR
GAGAGATAGACCTTGGCAGG+AGG 0.606963 5_1:-66482325 None:intergenic
GGTCAGCCAGATGATCTAAG+AGG 0.611676 5_1:-66484020 None:intergenic
ATGGAGCAAATAGACAATTG+TGG 0.612091 5_1:+66482554 Msa0745270:CDS
ACATCATGAAACATGCTCAC+AGG 0.612680 5_1:-66484200 None:intergenic
GTTCCTAGAGGTAACAAAGG+AGG 0.614577 5_1:+66482154 Msa0745270:five_prime_UTR
TGCAGGAACATACAACTACT+GGG 0.614884 5_1:+66483687 Msa0745270:CDS
AGAAGCTGCCTTGACAACAA+AGG 0.615553 5_1:-66482299 None:intergenic
CGGGATCTGAAAGTCCTAGA+GGG 0.617233 5_1:-66483492 None:intergenic
TCAGATCCCGAAGGAACTGG+TGG 0.619183 5_1:+66483505 Msa0745270:CDS
TCCGGCGAACCAGCTTACCC+TGG 0.621275 5_1:+66483844 Msa0745270:CDS
TCACGCCGGTTTGGAACCAA+GGG 0.621949 5_1:-66483657 None:intergenic
GGGTCAAATCCAAAGTCACC+AGG 0.625690 5_1:-66483472 None:intergenic
TTCTTGTGGTAGATATTGCA+TGG 0.626511 5_1:-66481998 None:intergenic
ACGTCCACGTTGCATCCACA+TGG 0.626539 5_1:+66482020 Msa0745270:five_prime_UTR
ATGCAACGTGGACGTTCTTG+TGG 0.631074 5_1:-66482012 None:intergenic
CAAAGCAGGTTTAATTCCCG+CGG 0.641267 5_1:+66483624 Msa0745270:CDS
TCAAGATGTTGTTGTTGACA+GGG 0.645710 5_1:-66484041 None:intergenic
TCCTGCAGGTGGAATCACGC+CGG 0.649684 5_1:-66483671 None:intergenic
CAAATCCAAAGTCACCAGGA+AGG 0.653377 5_1:-66483468 None:intergenic
CTGCTCCCAACATTGCGAAG+CGG 0.660409 5_1:-66483573 None:intergenic
CTAGGACTTTCAGATCCCGA+AGG 0.660487 5_1:+66483496 Msa0745270:CDS
TCGGGATCTGAAAGTCCTAG+AGG 0.663238 5_1:-66483493 None:intergenic
TCATCTTTACCAAAACCAAG+AGG 0.664345 5_1:-66483883 None:intergenic
GCATATGGCGAGATAATCAA+CGG 0.667486 5_1:+66483550 Msa0745270:CDS
TAACAAAGGAGGGAAGAACA+TGG 0.683240 5_1:+66482165 Msa0745270:exon
CTCTGCCAAATGTAGCCATG+TGG 0.685191 5_1:-66482035 None:intergenic
GGTTAAATATAGGTCCACCA+GGG 0.744381 5_1:-66483861 None:intergenic
GTAGCCATGTGGATGCAACG+TGG 0.793429 5_1:-66482024 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATATAAAAAATAAAAAAT+TGG - chr5_1:66483038-66483057 None:intergenic 0.0%
!!! TTTATTTTTTATATTACTTG+TGG + chr5_1:66483042-66483061 Msa0745270:intron 10.0%
!! AATAAATAAAACTTGTAATT+TGG + chr5_1:66484387-66484406 Msa0745270:three_prime_UTR 10.0%
!! TATATAATACATTCATTTAG+TGG - chr5_1:66484080-66484099 None:intergenic 15.0%
!! TAATATGATGAATTGGATAA+GGG - chr5_1:66481922-66481941 None:intergenic 20.0%
!!! ATAACTTTCTTTTCTCTTTT+TGG + chr5_1:66482520-66482539 Msa0745270:intron 20.0%
!! TTGAACAAAAAACTATTGTA+AGG + chr5_1:66482631-66482650 Msa0745270:intron 20.0%
!!! AGTATGAAATTGACTTTATT+CGG + chr5_1:66483427-66483446 Msa0745270:intron 20.0%
! GTAATATGATGAATTGGATA+AGG - chr5_1:66481923-66481942 None:intergenic 25.0%
! ATACACGTAATATGATGAAT+TGG - chr5_1:66481929-66481948 None:intergenic 25.0%
! TCATATTACGTGTATAAACA+TGG + chr5_1:66481934-66481953 Msa0745270:five_prime_UTR 25.0%
!!! AGTTTTTTGTTCAACTACTA+AGG - chr5_1:66482625-66482644 None:intergenic 25.0%
! AACAAAAAACTATTGTAAGG+TGG + chr5_1:66482634-66482653 Msa0745270:intron 25.0%
! CTTGATTAAACTGTTTACTT+AGG - chr5_1:66482696-66482715 None:intergenic 25.0%
! TCTACATGTTGTAATGTTAA+GGG - chr5_1:66482979-66482998 None:intergenic 25.0%
! ATCTACATGTTGTAATGTTA+AGG - chr5_1:66482980-66482999 None:intergenic 25.0%
! TGTCTTTAGATTATTTCGAT+AGG - chr5_1:66483239-66483258 None:intergenic 25.0%
! CAATCACTTGTCTAAATATT+TGG - chr5_1:66483305-66483324 None:intergenic 25.0%
! ATGTTGTATAGTTGTGTTAT+GGG - chr5_1:66483366-66483385 None:intergenic 25.0%
! TAAAGATGAGAAATCATTGA+AGG + chr5_1:66483897-66483916 Msa0745270:CDS 25.0%
ATTACGTGTATAAACATGGT+TGG + chr5_1:66481938-66481957 Msa0745270:five_prime_UTR 30.0%
TGAAGTAACTATAAGCCAAA+TGG - chr5_1:66482080-66482099 None:intergenic 30.0%
TTCTTCAAGAATGTAGTAGA+TGG - chr5_1:66482124-66482143 None:intergenic 30.0%
! AAGAAAAAACTTGTTCCTAG+AGG + chr5_1:66482142-66482161 Msa0745270:five_prime_UTR 30.0%
ATATTACAACTGTCTCATGT+GGG + chr5_1:66482407-66482426 Msa0745270:intron 30.0%
TTGTATATTTGTCAGTTCAG+TGG - chr5_1:66482473-66482492 None:intergenic 30.0%
AAAGCAAAGTCTTTCTTACT+TGG + chr5_1:66482585-66482604 Msa0745270:CDS 30.0%
ATGTAGATATGCAACTTTGT+TGG + chr5_1:66482992-66483011 Msa0745270:intron 30.0%
TTACTTGTGGTAACTTTGTA+TGG + chr5_1:66483055-66483074 Msa0745270:intron 30.0%
AAAATCTCAAAAGCCTTCTA+GGG - chr5_1:66483103-66483122 None:intergenic 30.0%
AAAAATCTCAAAAGCCTTCT+AGG - chr5_1:66483104-66483123 None:intergenic 30.0%
ATTGTGTTAGAACATAAGCT+TGG + chr5_1:66483321-66483340 Msa0745270:intron 30.0%
CATGTTGTATAGTTGTGTTA+TGG - chr5_1:66483367-66483386 None:intergenic 30.0%
!!! TAAGAAGTATTGTTTTCCCA+TGG - chr5_1:66483800-66483819 None:intergenic 30.0%
ATGGGAAAACAATACTTCTT+AGG + chr5_1:66483799-66483818 Msa0745270:CDS 30.0%
AAACCAAGAGGGTTAAATAT+AGG - chr5_1:66483874-66483893 None:intergenic 30.0%
! ATATTTAACCCTCTTGGTTT+TGG + chr5_1:66483874-66483893 Msa0745270:CDS 30.0%
TTAGCTATGTTGGCAATATT+GGG + chr5_1:66483952-66483971 Msa0745270:CDS 30.0%
! ATTCTTTTCTTTGCTCTTGT+TGG + chr5_1:66484108-66484127 Msa0745270:three_prime_UTR 30.0%
!!! AAAAGGTGGTTGAGTTTTTT+TGG - chr5_1:66484156-66484175 None:intergenic 30.0%
!!! TACCTTTTTTTTCGAGGTAA+AGG + chr5_1:66484418-66484437 Msa0745270:three_prime_UTR 30.0%
TACCTTTACCTCGAAAAAAA+AGG - chr5_1:66484423-66484442 None:intergenic 30.0%
AGCAAAAACGTAATAGCAGT+GGG + chr5_1:66481882-66481901 Msa0745270:five_prime_UTR 35.0%
TTCTTGTGGTAGATATTGCA+TGG - chr5_1:66482001-66482020 None:intergenic 35.0%
TCATATCTTCCTGATCCATT+TGG + chr5_1:66482062-66482081 Msa0745270:five_prime_UTR 35.0%
AACTATAAGCCAAATGGATC+AGG - chr5_1:66482074-66482093 None:intergenic 35.0%
TAGAAGACCCTATTGAAGAT+GGG - chr5_1:66482270-66482289 None:intergenic 35.0%
! TCAATAGGGTCTTCTAGAAA+AGG + chr5_1:66482274-66482293 Msa0745270:CDS 35.0%
! AAAAGGTTCCTTTGTTGTCA+AGG + chr5_1:66482291-66482310 Msa0745270:CDS 35.0%
CACATGAGACAGTTGTAATA+TGG - chr5_1:66482408-66482427 None:intergenic 35.0%
CATATTACAACTGTCTCATG+TGG + chr5_1:66482406-66482425 Msa0745270:intron 35.0%
!!! CTTTTTGGTGTTCAGCAAAA+TGG + chr5_1:66482535-66482554 Msa0745270:intron 35.0%
ATGGAGCAAATAGACAATTG+TGG + chr5_1:66482554-66482573 Msa0745270:CDS 35.0%
CAAAGTCTTTCTTACTTGGA+TGG + chr5_1:66482589-66482608 Msa0745270:CDS 35.0%
TGTTTACTTAGGCATACTCT+AGG - chr5_1:66482685-66482704 None:intergenic 35.0%
AGAACATAAGCTTGGATACT+TGG + chr5_1:66483329-66483348 Msa0745270:intron 35.0%
TAACTACACACTCTTTGTGA+TGG + chr5_1:66483714-66483733 Msa0745270:CDS 35.0%
AACACAGAAGATTCCAAGAT+TGG + chr5_1:66483761-66483780 Msa0745270:CDS 35.0%
TACTTCTTAGGACTTGAGAA+AGG + chr5_1:66483811-66483830 Msa0745270:CDS 35.0%
CATCTTTACCAAAACCAAGA+GGG - chr5_1:66483885-66483904 None:intergenic 35.0%
TCATCTTTACCAAAACCAAG+AGG - chr5_1:66483886-66483905 None:intergenic 35.0%
ATTGAAGGAGTTAAAGCTGA+AGG + chr5_1:66483912-66483931 Msa0745270:CDS 35.0%
AAGCTGAAGGAAGTTAAGAA+TGG + chr5_1:66483925-66483944 Msa0745270:CDS 35.0%
TGAAGGAAGTTAAGAATGGA+AGG + chr5_1:66483929-66483948 Msa0745270:CDS 35.0%
GTTAGCTATGTTGGCAATAT+TGG + chr5_1:66483951-66483970 Msa0745270:CDS 35.0%
TTTGTTCAAGCTCTTGTTAC+AGG + chr5_1:66483979-66483998 Msa0745270:CDS 35.0%
!! TGATCTAAGAGGTTTTGGTA+TGG - chr5_1:66484012-66484031 None:intergenic 35.0%
! TCAAGATGTTGTTGTTGACA+GGG - chr5_1:66484044-66484063 None:intergenic 35.0%
AAGACAAGAGAGTGAAGAAA+AGG - chr5_1:66484173-66484192 None:intergenic 35.0%
!! TGTACGTGTCATTTCTTACA+AGG - chr5_1:66484228-66484247 None:intergenic 35.0%
!!! ATCTGCTACCTTTTTTTTCG+AGG + chr5_1:66484412-66484431 Msa0745270:three_prime_UTR 35.0%
CAGCAAAAACGTAATAGCAG+TGG + chr5_1:66481881-66481900 Msa0745270:five_prime_UTR 40.0%
CTTGTTCCTAGAGGTAACAA+AGG + chr5_1:66482151-66482170 Msa0745270:five_prime_UTR 40.0%
TTCCTAGAGGTAACAAAGGA+GGG + chr5_1:66482155-66482174 Msa0745270:five_prime_UTR 40.0%
TAACAAAGGAGGGAAGAACA+TGG + chr5_1:66482165-66482184 Msa0745270:exon 40.0%
!! ATCTCTTACTTTTGCAGCTG+AGG + chr5_1:66482213-66482232 Msa0745270:CDS 40.0%
AGATCACTCCCATCTTCAAT+AGG + chr5_1:66482259-66482278 Msa0745270:CDS 40.0%
GATCACTCCCATCTTCAATA+GGG + chr5_1:66482260-66482279 Msa0745270:CDS 40.0%
CTAGAAGACCCTATTGAAGA+TGG - chr5_1:66482271-66482290 None:intergenic 40.0%
TTTGAGTGAGAGATAGACCT+TGG - chr5_1:66482335-66482354 None:intergenic 40.0%
GTCTTTCTTACTTGGATGGA+AGG + chr5_1:66482593-66482612 Msa0745270:CDS 40.0%
TTATGCAAGTGCATGTGTTG+AGG + chr5_1:66482921-66482940 Msa0745270:intron 40.0%
! TGTGAGTCTAAAACCCTAGA+AGG + chr5_1:66483087-66483106 Msa0745270:intron 40.0%
AATGAATCCACCAGTTCCTT+CGG - chr5_1:66483515-66483534 None:intergenic 40.0%
GCATATGGCGAGATAATCAA+CGG + chr5_1:66483550-66483569 Msa0745270:CDS 40.0%
! AATTTCAGGTGCTATAGCTC+CGG - chr5_1:66483599-66483618 None:intergenic 40.0%
GCTATAGCACCTGAAATTCT+CGG + chr5_1:66483601-66483620 Msa0745270:CDS 40.0%
TGCAGGAACATACAACTACT+GGG + chr5_1:66483687-66483706 Msa0745270:CDS 40.0%
! CACACTCTTTGTGATGGAAA+TGG + chr5_1:66483720-66483739 Msa0745270:CDS 40.0%
! TGTGATGGAAATGGCATTCA+TGG + chr5_1:66483729-66483748 Msa0745270:CDS 40.0%
! GTGATGGAAATGGCATTCAT+GGG + chr5_1:66483730-66483749 Msa0745270:CDS 40.0%
TTCCAAGATTGGTACAATCC+TGG + chr5_1:66483772-66483791 Msa0745270:CDS 40.0%
TTAGGACTTGAGAAAGGTCT+TGG + chr5_1:66483817-66483836 Msa0745270:CDS 40.0%
GGTTAAATATAGGTCCACCA+GGG - chr5_1:66483864-66483883 None:intergenic 40.0%
GGACCTATATTTAACCCTCT+TGG + chr5_1:66483868-66483887 Msa0745270:CDS 40.0%
! GAATGGAAGGTTAGCTATGT+TGG + chr5_1:66483942-66483961 Msa0745270:CDS 40.0%
! CCAGATGATCTAAGAGGTTT+TGG - chr5_1:66484017-66484036 None:intergenic 40.0%
CCAAAACCTCTTAGATCATC+TGG + chr5_1:66484014-66484033 Msa0745270:CDS 40.0%
! GTCAAGATGTTGTTGTTGAC+AGG - chr5_1:66484045-66484064 None:intergenic 40.0%
CATTTAGTGGAACTTGAGAC+TGG - chr5_1:66484067-66484086 None:intergenic 40.0%
! TTGCTCTTGTTGGTTGTTGT+AGG + chr5_1:66484118-66484137 Msa0745270:three_prime_UTR 40.0%
ACAAGAGAGTGAAGAAAAGG+TGG - chr5_1:66484170-66484189 None:intergenic 40.0%
ACATCATGAAACATGCTCAC+AGG - chr5_1:66484203-66484222 None:intergenic 40.0%
TGTGAGCATGTTTCATGATG+TGG + chr5_1:66484202-66484221 Msa0745270:three_prime_UTR 40.0%
TGCATCCACATGGCTACATT+TGG + chr5_1:66482030-66482049 Msa0745270:five_prime_UTR 45.0%
GTTCCTAGAGGTAACAAAGG+AGG + chr5_1:66482154-66482173 Msa0745270:five_prime_UTR 45.0%
TTCCCTCCTTTGTTACCTCT+AGG - chr5_1:66482160-66482179 None:intergenic 45.0%
AGAAGCTGCCTTGACAACAA+AGG - chr5_1:66482302-66482321 None:intergenic 45.0%
CAAATCCAAAGTCACCAGGA+AGG - chr5_1:66483471-66483490 None:intergenic 45.0%
GACTTTGGATTTGACCCTCT+AGG + chr5_1:66483478-66483497 Msa0745270:CDS 45.0%
ATGAATCCACCAGTTCCTTC+GGG - chr5_1:66483514-66483533 None:intergenic 45.0%
GTGGATTCATTGAGCCAAGA+TGG + chr5_1:66483524-66483543 Msa0745270:CDS 45.0%
GAGCCAAGATGGTTAGCATA+TGG + chr5_1:66483535-66483554 Msa0745270:CDS 45.0%
TCGCCATATGCTAACCATCT+TGG - chr5_1:66483541-66483560 None:intergenic 45.0%
CAAAGCAGGTTTAATTCCCG+CGG + chr5_1:66483624-66483643 Msa0745270:CDS 45.0%
TAGTTGTATGTTCCTGCAGG+TGG - chr5_1:66483685-66483704 None:intergenic 45.0%
CAGTAGTTGTATGTTCCTGC+AGG - chr5_1:66483688-66483707 None:intergenic 45.0%
CTGCAGGAACATACAACTAC+TGG + chr5_1:66483686-66483705 Msa0745270:CDS 45.0%
AGCCAGGATTGTACCAATCT+TGG - chr5_1:66483777-66483796 None:intergenic 45.0%
! TATTGTTTTCCCATGGAGCC+AGG - chr5_1:66483793-66483812 None:intergenic 45.0%
GGGTTAAATATAGGTCCACC+AGG - chr5_1:66483865-66483884 None:intergenic 45.0%
CAAGCTCTTGTTACAGGAGT+TGG + chr5_1:66483985-66484004 Msa0745270:CDS 45.0%
ATGCAACGTGGACGTTCTTG+TGG - chr5_1:66482015-66482034 None:intergenic 50.0%
CTCTGCCAAATGTAGCCATG+TGG - chr5_1:66482038-66482057 None:intergenic 50.0%
AGTGAGAGATAGACCTTGGC+AGG - chr5_1:66482331-66482350 None:intergenic 50.0%
ATCATCACTTGCAGCCTTCC+TGG + chr5_1:66483454-66483473 Msa0745270:intron 50.0%
GGGTCAAATCCAAAGTCACC+AGG - chr5_1:66483475-66483494 None:intergenic 50.0%
CGGGATCTGAAAGTCCTAGA+GGG - chr5_1:66483495-66483514 None:intergenic 50.0%
TCGGGATCTGAAAGTCCTAG+AGG - chr5_1:66483496-66483515 None:intergenic 50.0%
CTAGGACTTTCAGATCCCGA+AGG + chr5_1:66483496-66483515 Msa0745270:CDS 50.0%
CTTTCAGATCCCGAAGGAAC+TGG + chr5_1:66483502-66483521 Msa0745270:CDS 50.0%
CCTGCTTTGCCGAGAATTTC+AGG - chr5_1:66483613-66483632 None:intergenic 50.0%
CCTGAAATTCTCGGCAAAGC+AGG + chr5_1:66483610-66483629 Msa0745270:CDS 50.0%
TTGGTACAATCCTGGCTCCA+TGG + chr5_1:66483780-66483799 Msa0745270:CDS 50.0%
TGGTACAATCCTGGCTCCAT+GGG + chr5_1:66483781-66483800 Msa0745270:CDS 50.0%
GGACTTGAGAAAGGTCTTGG+AGG + chr5_1:66483820-66483839 Msa0745270:CDS 50.0%
GAGAAAGGTCTTGGAGGTTC+CGG + chr5_1:66483826-66483845 Msa0745270:CDS 50.0%
GGTCAGCCAGATGATCTAAG+AGG - chr5_1:66484023-66484042 None:intergenic 50.0%
ACGTCCACGTTGCATCCACA+TGG + chr5_1:66482020-66482039 Msa0745270:five_prime_UTR 55.0%
GTAGCCATGTGGATGCAACG+TGG - chr5_1:66482027-66482046 None:intergenic 55.0%
GAGGCTGTGAGACAGAGTCT+TGG + chr5_1:66482232-66482251 Msa0745270:CDS 55.0%
AGCTTCTTCTCCTCCTGCCA+AGG + chr5_1:66482315-66482334 Msa0745270:CDS 55.0%
GAGAGATAGACCTTGGCAGG+AGG - chr5_1:66482328-66482347 None:intergenic 55.0%
TGCAGCCTTCCTGGTGACTT+TGG + chr5_1:66483463-66483482 Msa0745270:intron 55.0%
TCAGATCCCGAAGGAACTGG+TGG + chr5_1:66483505-66483524 Msa0745270:CDS 55.0%
AACGGCCGCTTCGCAATGTT+GGG + chr5_1:66483568-66483587 Msa0745270:CDS 55.0%
CTGCTCCCAACATTGCGAAG+CGG - chr5_1:66483576-66483595 None:intergenic 55.0%
TTCGCAATGTTGGGAGCAGC+CGG + chr5_1:66483577-66483596 Msa0745270:CDS 55.0%
GCACTCCCTTGGTTCCAAAC+CGG + chr5_1:66483652-66483671 Msa0745270:CDS 55.0%
!! TCACGCCGGTTTGGAACCAA+GGG - chr5_1:66483660-66483679 None:intergenic 55.0%
!! ATCACGCCGGTTTGGAACCA+AGG - chr5_1:66483661-66483680 None:intergenic 55.0%
ATAGGTCCACCAGGGTAAGC+TGG - chr5_1:66483856-66483875 None:intergenic 55.0%
CAACGGCCGCTTCGCAATGT+TGG + chr5_1:66483567-66483586 Msa0745270:CDS 60.0%
!! CAAGGGAGTGCTGTTTCCGC+GGG - chr5_1:66483643-66483662 None:intergenic 60.0%
!! CCAAGGGAGTGCTGTTTCCG+CGG - chr5_1:66483644-66483663 None:intergenic 60.0%
! CAGGTGGAATCACGCCGGTT+TGG - chr5_1:66483669-66483688 None:intergenic 60.0%
TCCTGCAGGTGGAATCACGC+CGG - chr5_1:66483674-66483693 None:intergenic 60.0%
!! ACCAGGGTAAGCTGGTTCGC+CGG - chr5_1:66483848-66483867 None:intergenic 60.0%
CCGCGGAAACAGCACTCCCT+TGG + chr5_1:66483641-66483660 Msa0745270:CDS 65.0%
ACCGGCGTGATTCCACCTGC+AGG + chr5_1:66483670-66483689 Msa0745270:CDS 65.0%
TCCGGCGAACCAGCTTACCC+TGG + chr5_1:66483844-66483863 Msa0745270:CDS 65.0%
GGCGAACCAGCTTACCCTGG+TGG + chr5_1:66483847-66483866 Msa0745270:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 66481866 66484461 66481866 ID=Msa0745270;Name=Msa0745270
chr5_1 mRNA 66481866 66484461 66481866 ID=Msa0745270-mRNA-1;Parent=Msa0745270;Name=Msa0745270-mRNA-1;_AED=0.03;_eAED=0.03;_QI=318|1|1|1|1|1|3|378|277
chr5_1 exon 66481866 66482336 66481866 ID=Msa0745270-mRNA-1:exon:19771;Parent=Msa0745270-mRNA-1
chr5_1 exon 66482550 66482614 66482550 ID=Msa0745270-mRNA-1:exon:19772;Parent=Msa0745270-mRNA-1
chr5_1 exon 66483468 66484461 66483468 ID=Msa0745270-mRNA-1:exon:19773;Parent=Msa0745270-mRNA-1
chr5_1 five_prime_UTR 66481866 66482183 66481866 ID=Msa0745270-mRNA-1:five_prime_utr;Parent=Msa0745270-mRNA-1
chr5_1 CDS 66482184 66482336 66482184 ID=Msa0745270-mRNA-1:cds;Parent=Msa0745270-mRNA-1
chr5_1 CDS 66482550 66482614 66482550 ID=Msa0745270-mRNA-1:cds;Parent=Msa0745270-mRNA-1
chr5_1 CDS 66483468 66484083 66483468 ID=Msa0745270-mRNA-1:cds;Parent=Msa0745270-mRNA-1
chr5_1 three_prime_UTR 66484084 66484461 66484084 ID=Msa0745270-mRNA-1:three_prime_utr;Parent=Msa0745270-mRNA-1
Gene Sequence

>Msa0745270

ATGGCTACACAAGCTCTAGTGTCGTCATCATCTCTTACTTTTGCAGCTGAGGCTGTGAGACAGAGTCTTGGAGCAAGATCACTCCCATCTTCAATAGGGTCTTCTAGAAAAGGTTCCTTTGTTGTCAAGGCAGCTTCTTCTCCTCCTGCCAAGCAAAATGGAGCAAATAGACAATTGTGGTTTGCATCAAAGCAAAGTCTTTCTTACTTGGATGGAAGCCTTCCTGGTGACTTTGGATTTGACCCTCTAGGACTTTCAGATCCCGAAGGAACTGGTGGATTCATTGAGCCAAGATGGTTAGCATATGGCGAGATAATCAACGGCCGCTTCGCAATGTTGGGAGCAGCCGGAGCTATAGCACCTGAAATTCTCGGCAAAGCAGGTTTAATTCCCGCGGAAACAGCACTCCCTTGGTTCCAAACCGGCGTGATTCCACCTGCAGGAACATACAACTACTGGGCAGATAACTACACACTCTTTGTGATGGAAATGGCATTCATGGGATTTGCAGAACACAGAAGATTCCAAGATTGGTACAATCCTGGCTCCATGGGAAAACAATACTTCTTAGGACTTGAGAAAGGTCTTGGAGGTTCCGGCGAACCAGCTTACCCTGGTGGACCTATATTTAACCCTCTTGGTTTTGGTAAAGATGAGAAATCATTGAAGGAGTTAAAGCTGAAGGAAGTTAAGAATGGAAGGTTAGCTATGTTGGCAATATTGGGTTACTTTGTTCAAGCTCTTGTTACAGGAGTTGGACCATACCAAAACCTCTTAGATCATCTGGCTGACCCTGTCAACAACAACATCTTGACCAGTCTCAAGTTCCACTAA
Protein sequence

>Msa0745270

MATQALVSSSSLTFAAEAVRQSLGARSLPSSIGSSRKGSFVVKAASSPPAKQNGANRQLWFASKQSLSYLDGSLPGDFGFDPLGLSDPEGTGGFIEPRWLAYGEIINGRFAMLGAAGAIAPEILGKAGLIPAETALPWFQTGVIPPAGTYNYWADNYTLFVMEMAFMGFAEHRRFQDWYNPGSMGKQYFLGLEKGLGGSGEPAYPGGPIFNPLGFGKDEKSLKELKLKEVKNGRLAMLAILGYFVQALVTGVGPYQNLLDHLADPVNNNILTSLKFH*