AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1365200


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1365200 MtrunA17_Chr4g0072121 96.026 151 6 0 1 151 1 151 6.70e-105 296
Msa1365200 MtrunA17_Chr2g0298051 51.977 177 56 7 3 150 2 178 3.15e-50 158
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1365200 AT2G18969.1 40.230 174 81 4 1 151 1 174 2.20e-33 116
Msa1365200 AT4G30180.1 38.509 161 86 2 1 151 1 158 6.61e-28 101

Find 15 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTTATCTTCATCAATGC+TGG 0.377349 tig0018212:-10956 None:intergenic
TCCAGCATTGATGAAGATAA+AGG 0.403965 tig0018212:+10955 Msa1365200:CDS
AGAAGATGAAGAACTTGATA+AGG 0.412000 tig0018212:+11167 Msa1365200:CDS
TATAAGATGTTTACAAATGC+AGG 0.434952 tig0018212:+11249 Msa1365200:CDS
GGAAAATAATATTTCTTCAA+AGG 0.459877 tig0018212:+10976 Msa1365200:CDS
CCTTAAACTCAAGCTTCAAA+AGG 0.493255 tig0018212:+11075 Msa1365200:CDS
TAAGAATGTTAAGTATGAAG+TGG 0.531002 tig0018212:+11006 Msa1365200:CDS
GAATGAAGCTAGTGAAGATG+AGG 0.535484 tig0018212:+11144 Msa1365200:CDS
GTATGAAGTGGACATGGCAA+TGG 0.553114 tig0018212:+11018 Msa1365200:CDS
ACGTAGACGTGTTTATTCTG+TGG 0.593083 tig0018212:+10877 Msa1365200:CDS
ATCTTGATTAAACATGGAGG+AGG 0.595978 tig0018212:+10841 None:intergenic
TCACAAATCTCTTCTCCACC+AGG 0.612567 tig0018212:-11196 None:intergenic
TGTTAAGTATGAAGTGGACA+TGG 0.621830 tig0018212:+11012 Msa1365200:CDS
AACTTGATAAGGTTGATACC+TGG 0.636291 tig0018212:+11178 Msa1365200:CDS
TTGATAAGGTTGATACCTGG+TGG 0.649046 tig0018212:+11181 Msa1365200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GGAAAATAATATTTCTTCAA+AGG + tig0018212:10976-10995 Msa1365200:CDS 20.0%
!!! ATAGTTTGCACTATTTTGTT+TGG - tig0018212:10905-10924 None:intergenic 25.0%
! TAAGAATGTTAAGTATGAAG+TGG + tig0018212:11006-11025 Msa1365200:CDS 25.0%
! TATAAGATGTTTACAAATGC+AGG + tig0018212:11249-11268 Msa1365200:CDS 25.0%
AGAAGATGAAGAACTTGATA+AGG + tig0018212:11167-11186 Msa1365200:CDS 30.0%
!! TCCAGCATTGATGAAGATAA+AGG + tig0018212:10955-10974 Msa1365200:CDS 35.0%
TCCTTTATCTTCATCAATGC+TGG - tig0018212:10959-10978 None:intergenic 35.0%
TGTTAAGTATGAAGTGGACA+TGG + tig0018212:11012-11031 Msa1365200:CDS 35.0%
!!! CCTTTTGAAGCTTGAGTTTA+AGG - tig0018212:11078-11097 None:intergenic 35.0%
CCTTAAACTCAAGCTTCAAA+AGG + tig0018212:11075-11094 Msa1365200:CDS 35.0%
!! AACTTGATAAGGTTGATACC+TGG + tig0018212:11178-11197 Msa1365200:CDS 35.0%
ACGTAGACGTGTTTATTCTG+TGG + tig0018212:10877-10896 Msa1365200:CDS 40.0%
GAATGAAGCTAGTGAAGATG+AGG + tig0018212:11144-11163 Msa1365200:CDS 40.0%
!! TTGATAAGGTTGATACCTGG+TGG + tig0018212:11181-11200 Msa1365200:CDS 40.0%
GTATGAAGTGGACATGGCAA+TGG + tig0018212:11018-11037 Msa1365200:CDS 45.0%
!!! GCAATGGTTTTGTCAGCACA+AGG + tig0018212:11034-11053 Msa1365200:CDS 45.0%
! TGTCAGCACAAGGTTTTGCA+TGG + tig0018212:11044-11063 Msa1365200:CDS 45.0%
! CAAGGTTTTGCATGGAGTGA+TGG + tig0018212:11052-11071 Msa1365200:CDS 45.0%
TCACAAATCTCTTCTCCACC+AGG - tig0018212:11199-11218 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
tig0018212 gene 10854 11309 10854 ID=Msa1365200;Name=Msa1365200
tig0018212 mRNA 10854 11309 10854 ID=Msa1365200-mRNA-1;Parent=Msa1365200;Name=Msa1365200-mRNA-1;_AED=0.03;_eAED=0.04;_QI=0|-1|0|1|-1|1|1|0|151
tig0018212 exon 10854 11309 10854 ID=Msa1365200-mRNA-1:exon:14421;Parent=Msa1365200-mRNA-1
tig0018212 CDS 10854 11309 10854 ID=Msa1365200-mRNA-1:cds;Parent=Msa1365200-mRNA-1
Gene Sequence

>Msa1365200

ATGGAGGAGGCACAACAAAATAAACGTAGACGTGTTTATTCTGTGGAACCAAACAAAATAGTGCAAACTATATTTGCTAGAAACTATTTAAGTTATTTAGTTCCAGCATTGATGAAGATAAAGGAAAATAATATTTCTTCAAAGGAAAATATTAAGAATGTTAAGTATGAAGTGGACATGGCAATGGTTTTGTCAGCACAAGGTTTTGCATGGAGTGATGGCCTTAAACTCAAGCTTCAAAAGGAATGTAATGTCAATGAAGTAGCTACAAACAGTAGAAGCATAATTGAGAATGAAGCTAGTGAAGATGAGGAGAAGATGAAGAACTTGATAAGGTTGATACCTGGTGGAGAAGAGATTTGTGATGAACAAGTTGTTAATGAATTAGAAAGTTATATAAGATGTTTACAAATGCAGGTGAATGTTCTTCAATGTTTAATAGCAGAGACATGTTGA

Protein sequence

>Msa1365200

MEEAQQNKRRRVYSVEPNKIVQTIFARNYLSYLVPALMKIKENNISSKENIKNVKYEVDMAMVLSAQGFAWSDGLKLKLQKECNVNEVATNSRSIIENEASEDEEKMKNLIRLIPGGEEICDEQVVNELESYIRCLQMQVNVLQCLIAETC*