AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0850500


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0850500 MtrunA17_Chr5g0425991 91.324 219 13 1 1 219 1 213 1.20e-140 392
Msa0850500 MtrunA17_Chr3g0115791 58.333 168 47 9 64 218 2 159 9.20e-48 154
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0850500 AT1G78170.1 36.522 230 109 10 1 211 1 212 1.50e-25 99.8
Msa0850500 AT4G08910.1 39.286 112 52 2 108 203 86 197 2.33e-17 77.8
Msa0850500 AT1G22250.1 42.609 115 59 2 111 218 85 199 2.56e-17 77.4

Find 39 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCACATAGACTTATTTAT+TGG 0.127321 5_4:-39777568 None:intergenic
TTCTGATCAAGAATTCTTTA+AGG 0.147055 5_4:+39776765 Msa0850500:CDS
CACCTTTGCCTTCAGATAAA+TGG 0.203598 5_4:+39776848 Msa0850500:CDS
TTAAGAACATACATAAATTT+AGG 0.233603 5_4:-39777776 None:intergenic
CTTCACAAAAGAAGAGAAAA+TGG 0.266395 5_4:+39776728 Msa0850500:CDS
GCGAAGTTCTATATCAAATA+TGG 0.295622 5_4:-39776818 None:intergenic
AAACCACTCTTGTTTGAATA+TGG 0.302002 5_4:+39776645 Msa0850500:exon
TATTATATATTTAAAACAAA+AGG 0.330628 5_4:+39777614 Msa0850500:three_prime_UTR
CAAGAACAACCACTTCTAAT+TGG 0.345178 5_4:+39777544 Msa0850500:three_prime_UTR
ATACATAAATATGATGATTA+TGG 0.348413 5_4:-39777728 None:intergenic
ATCTATTAGCAAGAATAAAA+AGG 0.355406 5_4:+39777299 Msa0850500:CDS
AAGGATCTTACACATGATCT+AGG 0.356603 5_4:+39776784 Msa0850500:CDS
GGATGCATGAATTTGCAATT+AGG 0.374086 5_4:-39777459 None:intergenic
CAAATTATACAACTCCTTAG+AGG 0.387146 5_4:-39777658 None:intergenic
ATTATATATTTAAAACAAAA+GGG 0.432410 5_4:+39777615 Msa0850500:three_prime_UTR
TTAATCTACCTCTACCTCTA+AGG 0.434008 5_4:+39777644 Msa0850500:three_prime_UTR
GTATGTTTCTTAATGCTGTA+AGG 0.449888 5_4:+39777806 Msa0850500:three_prime_UTR
GCAACAATACCTCACAGTTC+AGG 0.455526 5_4:+39776870 Msa0850500:CDS
AATTTGGAAGGGTTCTGATC+TGG 0.466878 5_4:-39777486 None:intergenic
GAAGGGTTCTGATCTGGTGG+TGG 0.491999 5_4:-39777480 None:intergenic
AAGAATTCTTGATCAGAAAG+TGG 0.498028 5_4:-39776760 None:intergenic
AAGGAGTTGTATAATTTGAC+TGG 0.504963 5_4:+39777663 Msa0850500:three_prime_UTR
TAAGTACTACTAATTCATCA+TGG 0.522676 5_4:+39777334 Msa0850500:CDS
TTCACAAAAGAAGAGAAAAT+GGG 0.522716 5_4:+39776729 Msa0850500:CDS
ATACAACTCCTTAGAGGTAG+AGG 0.527574 5_4:-39777652 None:intergenic
TATTGTTGCCATTTATCTGA+AGG 0.533510 5_4:-39776856 None:intergenic
AGGATCTTACACATGATCTA+GGG 0.553583 5_4:+39776785 Msa0850500:CDS
TGTGAAGCATCAAAATCTGT+AGG 0.557840 5_4:-39776712 None:intergenic
CACATGTCAAATTAAGCTCA+AGG 0.558418 5_4:-39777218 None:intergenic
AATTTGCAATTAGGACAAGA+AGG 0.564845 5_4:-39777450 None:intergenic
TTTATTGGACCAATTAGAAG+TGG 0.571145 5_4:-39777553 None:intergenic
TTGGAAGGGTTCTGATCTGG+TGG 0.597114 5_4:-39777483 None:intergenic
TGCCATTTATCTGAAGGCAA+AGG 0.597180 5_4:-39776850 None:intergenic
TGGTTATCATCATCATCAGA+TGG 0.614533 5_4:+39777354 Msa0850500:CDS
TATTTATGTATATATAAGAG+TGG 0.623638 5_4:+39777740 Msa0850500:three_prime_UTR
AGACCATATTCAAACAAGAG+TGG 0.639805 5_4:-39776648 None:intergenic
TATGATGATTATGGTACACA+AGG 0.666866 5_4:-39777719 None:intergenic
TTGTATGAAGTGTCACATGT+TGG 0.683627 5_4:+39777407 Msa0850500:CDS
TATTATATACCTGAACTGTG+AGG 0.723748 5_4:-39776879 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAATATATTAACCT+CGG - chr5_4:39776958-39776977 None:intergenic 10.0%
!! AAAATAAAATATATTAACCT+CGG - chr5_4:39776958-39776977 None:intergenic 10.0%
!!! GAGGTTAATATATTTTATTT+TGG + chr5_4:39776957-39776976 Msa0850500:intron 15.0%
!! TTATAATATTATTGCTACAT+CGG - chr5_4:39777004-39777023 None:intergenic 15.0%
!! ATACATAAATATGATGATTA+TGG - chr5_4:39777731-39777750 None:intergenic 15.0%
!! TATTTATGTATATATAAGAG+TGG + chr5_4:39777740-39777759 Msa0850500:three_prime_UTR 15.0%
!! TTAAGAACATACATAAATTT+AGG - chr5_4:39777779-39777798 None:intergenic 15.0%
!!! GAGGTTAATATATTTTATTT+TGG + chr5_4:39776957-39776976 Msa0850500:intron 15.0%
!! TTATAATATTATTGCTACAT+CGG - chr5_4:39777004-39777023 None:intergenic 15.0%
!! ATACATAAATATGATGATTA+TGG - chr5_4:39777731-39777750 None:intergenic 15.0%
!! TATTTATGTATATATAAGAG+TGG + chr5_4:39777740-39777759 Msa0850500:three_prime_UTR 15.0%
!! TTAAGAACATACATAAATTT+AGG - chr5_4:39777779-39777798 None:intergenic 15.0%
!! ATCTATTAGCAAGAATAAAA+AGG + chr5_4:39777299-39777318 Msa0850500:CDS 20.0%
!! AATCACATAGACTTATTTAT+TGG - chr5_4:39777571-39777590 None:intergenic 20.0%
!! ATCTATTAGCAAGAATAAAA+AGG + chr5_4:39777299-39777318 Msa0850500:CDS 20.0%
!! AATCACATAGACTTATTTAT+TGG - chr5_4:39777571-39777590 None:intergenic 20.0%
! TTCACAAAAGAAGAGAAAAT+GGG + chr5_4:39776729-39776748 Msa0850500:CDS 25.0%
! TTCTGATCAAGAATTCTTTA+AGG + chr5_4:39776765-39776784 Msa0850500:CDS 25.0%
! CGAAAATGTGCTTATAAAAA+GGG - chr5_4:39776914-39776933 None:intergenic 25.0%
! TAAGTACTACTAATTCATCA+TGG + chr5_4:39777334-39777353 Msa0850500:CDS 25.0%
! TTCTCTTCAAAAATTTGGAA+GGG - chr5_4:39777500-39777519 None:intergenic 25.0%
! TTCACAAAAGAAGAGAAAAT+GGG + chr5_4:39776729-39776748 Msa0850500:CDS 25.0%
! TTCTGATCAAGAATTCTTTA+AGG + chr5_4:39776765-39776784 Msa0850500:CDS 25.0%
! CGAAAATGTGCTTATAAAAA+GGG - chr5_4:39776914-39776933 None:intergenic 25.0%
! TAAGTACTACTAATTCATCA+TGG + chr5_4:39777334-39777353 Msa0850500:CDS 25.0%
! TTCTCTTCAAAAATTTGGAA+GGG - chr5_4:39777500-39777519 None:intergenic 25.0%
! AAACCACTCTTGTTTGAATA+TGG + chr5_4:39776645-39776664 Msa0850500:exon 30.0%
CTTCACAAAAGAAGAGAAAA+TGG + chr5_4:39776728-39776747 Msa0850500:CDS 30.0%
AAGAATTCTTGATCAGAAAG+TGG - chr5_4:39776763-39776782 None:intergenic 30.0%
GCGAAGTTCTATATCAAATA+TGG - chr5_4:39776821-39776840 None:intergenic 30.0%
! TATTGTTGCCATTTATCTGA+AGG - chr5_4:39776859-39776878 None:intergenic 30.0%
TATTATATACCTGAACTGTG+AGG - chr5_4:39776882-39776901 None:intergenic 30.0%
GCGAAAATGTGCTTATAAAA+AGG - chr5_4:39776915-39776934 None:intergenic 30.0%
! TTAAATTTTCTCATGCAGTC+AGG + chr5_4:39777093-39777112 Msa0850500:intron 30.0%
! TAAATTTTCTCATGCAGTCA+GGG + chr5_4:39777094-39777113 Msa0850500:intron 30.0%
!!! GAGATGATTTTTCAGGTAAA+GGG - chr5_4:39777179-39777198 None:intergenic 30.0%
!!! AGAGATGATTTTTCAGGTAA+AGG - chr5_4:39777180-39777199 None:intergenic 30.0%
TTACCTGAAAAATCATCTCT+AGG + chr5_4:39777180-39777199 Msa0850500:CDS 30.0%
AATTTGCAATTAGGACAAGA+AGG - chr5_4:39777453-39777472 None:intergenic 30.0%
GTTCTCTTCAAAAATTTGGA+AGG - chr5_4:39777501-39777520 None:intergenic 30.0%
CCTTGTTCTCTTCAAAAATT+TGG - chr5_4:39777505-39777524 None:intergenic 30.0%
!! CCAAATTTTTGAAGAGAACA+AGG + chr5_4:39777502-39777521 Msa0850500:CDS 30.0%
TTTATTGGACCAATTAGAAG+TGG - chr5_4:39777556-39777575 None:intergenic 30.0%
CAAATTATACAACTCCTTAG+AGG - chr5_4:39777661-39777680 None:intergenic 30.0%
AAGGAGTTGTATAATTTGAC+TGG + chr5_4:39777663-39777682 Msa0850500:three_prime_UTR 30.0%
TATGATGATTATGGTACACA+AGG - chr5_4:39777722-39777741 None:intergenic 30.0%
GTATGTTTCTTAATGCTGTA+AGG + chr5_4:39777806-39777825 Msa0850500:three_prime_UTR 30.0%
! TTTTCTCAACTCTTTCTTTC+TGG - chr5_4:39776628-39776647 None:intergenic 30.0%
! AAACCACTCTTGTTTGAATA+TGG + chr5_4:39776645-39776664 Msa0850500:exon 30.0%
CTTCACAAAAGAAGAGAAAA+TGG + chr5_4:39776728-39776747 Msa0850500:CDS 30.0%
AAGAATTCTTGATCAGAAAG+TGG - chr5_4:39776763-39776782 None:intergenic 30.0%
GCGAAGTTCTATATCAAATA+TGG - chr5_4:39776821-39776840 None:intergenic 30.0%
! TATTGTTGCCATTTATCTGA+AGG - chr5_4:39776859-39776878 None:intergenic 30.0%
TATTATATACCTGAACTGTG+AGG - chr5_4:39776882-39776901 None:intergenic 30.0%
GCGAAAATGTGCTTATAAAA+AGG - chr5_4:39776915-39776934 None:intergenic 30.0%
! TTAAATTTTCTCATGCAGTC+AGG + chr5_4:39777093-39777112 Msa0850500:intron 30.0%
! TAAATTTTCTCATGCAGTCA+GGG + chr5_4:39777094-39777113 Msa0850500:intron 30.0%
!!! GAGATGATTTTTCAGGTAAA+GGG - chr5_4:39777179-39777198 None:intergenic 30.0%
!!! AGAGATGATTTTTCAGGTAA+AGG - chr5_4:39777180-39777199 None:intergenic 30.0%
TTACCTGAAAAATCATCTCT+AGG + chr5_4:39777180-39777199 Msa0850500:CDS 30.0%
AATTTGCAATTAGGACAAGA+AGG - chr5_4:39777453-39777472 None:intergenic 30.0%
GTTCTCTTCAAAAATTTGGA+AGG - chr5_4:39777501-39777520 None:intergenic 30.0%
CCTTGTTCTCTTCAAAAATT+TGG - chr5_4:39777505-39777524 None:intergenic 30.0%
!! CCAAATTTTTGAAGAGAACA+AGG + chr5_4:39777502-39777521 Msa0850500:CDS 30.0%
TTTATTGGACCAATTAGAAG+TGG - chr5_4:39777556-39777575 None:intergenic 30.0%
CAAATTATACAACTCCTTAG+AGG - chr5_4:39777661-39777680 None:intergenic 30.0%
AAGGAGTTGTATAATTTGAC+TGG + chr5_4:39777663-39777682 Msa0850500:three_prime_UTR 30.0%
TATGATGATTATGGTACACA+AGG - chr5_4:39777722-39777741 None:intergenic 30.0%
GTATGTTTCTTAATGCTGTA+AGG + chr5_4:39777806-39777825 Msa0850500:three_prime_UTR 30.0%
AGACCATATTCAAACAAGAG+TGG - chr5_4:39776651-39776670 None:intergenic 35.0%
ACTAACCATGAGTCCAAAAA+AGG + chr5_4:39776684-39776703 Msa0850500:CDS 35.0%
!!! AGATTCCTTTTTTGGACTCA+TGG - chr5_4:39776692-39776711 None:intergenic 35.0%
!!! TCTGTAGGAGATTCCTTTTT+TGG - chr5_4:39776700-39776719 None:intergenic 35.0%
! TGTGAAGCATCAAAATCTGT+AGG - chr5_4:39776715-39776734 None:intergenic 35.0%
AAGGATCTTACACATGATCT+AGG + chr5_4:39776784-39776803 Msa0850500:CDS 35.0%
AGGATCTTACACATGATCTA+GGG + chr5_4:39776785-39776804 Msa0850500:CDS 35.0%
!! CTAGTTTTTAATTAGAGCCG+AGG + chr5_4:39776938-39776957 Msa0850500:intron 35.0%
! TGACCTAGAGATGATTTTTC+AGG - chr5_4:39777186-39777205 None:intergenic 35.0%
CACATGTCAAATTAAGCTCA+AGG - chr5_4:39777221-39777240 None:intergenic 35.0%
TGGTTATCATCATCATCAGA+TGG + chr5_4:39777354-39777373 Msa0850500:CDS 35.0%
! TTGTATGAAGTGTCACATGT+TGG + chr5_4:39777407-39777426 Msa0850500:CDS 35.0%
GGATGCATGAATTTGCAATT+AGG - chr5_4:39777462-39777481 None:intergenic 35.0%
CAAGAACAACCACTTCTAAT+TGG + chr5_4:39777544-39777563 Msa0850500:three_prime_UTR 35.0%
TTAATCTACCTCTACCTCTA+AGG + chr5_4:39777644-39777663 Msa0850500:three_prime_UTR 35.0%
AGACCATATTCAAACAAGAG+TGG - chr5_4:39776651-39776670 None:intergenic 35.0%
ACTAACCATGAGTCCAAAAA+AGG + chr5_4:39776684-39776703 Msa0850500:CDS 35.0%
!!! AGATTCCTTTTTTGGACTCA+TGG - chr5_4:39776692-39776711 None:intergenic 35.0%
!!! TCTGTAGGAGATTCCTTTTT+TGG - chr5_4:39776700-39776719 None:intergenic 35.0%
! TGTGAAGCATCAAAATCTGT+AGG - chr5_4:39776715-39776734 None:intergenic 35.0%
AAGGATCTTACACATGATCT+AGG + chr5_4:39776784-39776803 Msa0850500:CDS 35.0%
AGGATCTTACACATGATCTA+GGG + chr5_4:39776785-39776804 Msa0850500:CDS 35.0%
!! CTAGTTTTTAATTAGAGCCG+AGG + chr5_4:39776938-39776957 Msa0850500:intron 35.0%
! TGACCTAGAGATGATTTTTC+AGG - chr5_4:39777186-39777205 None:intergenic 35.0%
CACATGTCAAATTAAGCTCA+AGG - chr5_4:39777221-39777240 None:intergenic 35.0%
TGGTTATCATCATCATCAGA+TGG + chr5_4:39777354-39777373 Msa0850500:CDS 35.0%
! TTGTATGAAGTGTCACATGT+TGG + chr5_4:39777407-39777426 Msa0850500:CDS 35.0%
GGATGCATGAATTTGCAATT+AGG - chr5_4:39777462-39777481 None:intergenic 35.0%
CAAGAACAACCACTTCTAAT+TGG + chr5_4:39777544-39777563 Msa0850500:three_prime_UTR 35.0%
TTAATCTACCTCTACCTCTA+AGG + chr5_4:39777644-39777663 Msa0850500:three_prime_UTR 35.0%
CACCTTTGCCTTCAGATAAA+TGG + chr5_4:39776848-39776867 Msa0850500:CDS 40.0%
! TGCCATTTATCTGAAGGCAA+AGG - chr5_4:39776853-39776872 None:intergenic 40.0%
! AATTTGGAAGGGTTCTGATC+TGG - chr5_4:39777489-39777508 None:intergenic 40.0%
ATACAACTCCTTAGAGGTAG+AGG - chr5_4:39777655-39777674 None:intergenic 40.0%
CACCTTTGCCTTCAGATAAA+TGG + chr5_4:39776848-39776867 Msa0850500:CDS 40.0%
! TGCCATTTATCTGAAGGCAA+AGG - chr5_4:39776853-39776872 None:intergenic 40.0%
! AATTTGGAAGGGTTCTGATC+TGG - chr5_4:39777489-39777508 None:intergenic 40.0%
ATACAACTCCTTAGAGGTAG+AGG - chr5_4:39777655-39777674 None:intergenic 40.0%
!!! CTGAAGGCAAAGGTGTTTTG+AGG - chr5_4:39776843-39776862 None:intergenic 45.0%
GCAACAATACCTCACAGTTC+AGG + chr5_4:39776870-39776889 Msa0850500:CDS 45.0%
!!! CTGAAGGCAAAGGTGTTTTG+AGG - chr5_4:39776843-39776862 None:intergenic 45.0%
GCAACAATACCTCACAGTTC+AGG + chr5_4:39776870-39776889 Msa0850500:CDS 45.0%
!!! TATTATATATTTAAAACAAA+AGG + chr5_4:39777614-39777633 Msa0850500:three_prime_UTR 5.0%
!!! ATTATATATTTAAAACAAAA+GGG + chr5_4:39777615-39777634 Msa0850500:three_prime_UTR 5.0%
!!! TATTATATATTTAAAACAAA+AGG + chr5_4:39777614-39777633 Msa0850500:three_prime_UTR 5.0%
!!! ATTATATATTTAAAACAAAA+GGG + chr5_4:39777615-39777634 Msa0850500:three_prime_UTR 5.0%
! TTGGAAGGGTTCTGATCTGG+TGG - chr5_4:39777486-39777505 None:intergenic 50.0%
! TTGGAAGGGTTCTGATCTGG+TGG - chr5_4:39777486-39777505 None:intergenic 50.0%
! GAAGGGTTCTGATCTGGTGG+TGG - chr5_4:39777483-39777502 None:intergenic 55.0%
! GAAGGGTTCTGATCTGGTGG+TGG - chr5_4:39777483-39777502 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 39776628 39777861 39776628 ID=Msa0850500;Name=Msa0850500
chr5_4 mRNA 39776628 39777861 39776628 ID=Msa0850500-mRNA-1;Parent=Msa0850500;Name=Msa0850500-mRNA-1;_AED=0.02;_eAED=0.02;_QI=36|1|1|1|1|1|2|319|219
chr5_4 exon 39776628 39776891 39776628 ID=Msa0850500-mRNA-1:exon:12608;Parent=Msa0850500-mRNA-1
chr5_4 exon 39777111 39777861 39777111 ID=Msa0850500-mRNA-1:exon:12609;Parent=Msa0850500-mRNA-1
chr5_4 five_prime_UTR 39776628 39776663 39776628 ID=Msa0850500-mRNA-1:five_prime_utr;Parent=Msa0850500-mRNA-1
chr5_4 CDS 39776664 39776891 39776664 ID=Msa0850500-mRNA-1:cds;Parent=Msa0850500-mRNA-1
chr5_4 CDS 39777111 39777542 39777111 ID=Msa0850500-mRNA-1:cds;Parent=Msa0850500-mRNA-1
chr5_4 three_prime_UTR 39777543 39777861 39777543 ID=Msa0850500-mRNA-1:three_prime_utr;Parent=Msa0850500-mRNA-1
Gene Sequence

>Msa0850500

ATGGTCTCACTTCACAAAGCACTAACCATGAGTCCAAAAAAGGAATCTCCTACAGATTTTGATGCTTCACAAAAGAAGAGAAAATGGGAAGAATCACCACTTTCTGATCAAGAATTCTTTAAGGATCTTACACATGATCTAGGGAAAAGAAAATCCATATTTGATATAGAACTTCGCCTCAAAACACCTTTGCCTTCAGATAAATGGCAACAATACCTCACAGTTCAGTCAGGGCAAATACAATTGTGTAACACAAAGATGAATATGAAAACACAAGAATCAAAAAGAGAGTACCCTTTACCTGAAAAATCATCTCTAGGTCATTTAAGCTTAGACCTTGAGCTTAATTTGACATGTGAATCATTGAAGAAAAAAGAAGAAAGTTATTTTGAAAAGAAGAATAATCTAGTGAATGAATCTATTAGCAAGAATAAAAAGGATTCAAGTTTTTTAAGTACTACTAATTCATCATGGTTATCATCATCATCAGATGGTGATGATTACAAAGAAATGATTGCAACAGTTTGTATGAAGTGTCACATGTTGGTAATGCTTTGCAAATCATCTCCTTCTTGTCCTAATTGCAAATTCATGCATCCACCACCAGATCAGAACCCTTCCAAATTTTTGAAGAGAACAAGGTTTAGTTTCTTGACATAA

Protein sequence

>Msa0850500

MVSLHKALTMSPKKESPTDFDASQKKRKWEESPLSDQEFFKDLTHDLGKRKSIFDIELRLKTPLPSDKWQQYLTVQSGQIQLCNTKMNMKTQESKREYPLPEKSSLGHLSLDLELNLTCESLKKKEESYFEKKNNLVNESISKNKKDSSFLSTTNSSWLSSSSDGDDYKEMIATVCMKCHMLVMLCKSSPSCPNCKFMHPPPDQNPSKFLKRTRFSFLT*