AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0669190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0669190 MtrunA17_Chr4g0008411 95.939 197 8 0 1 197 1 197 1.44e-136 382
Msa0669190 MtrunA17_Chr4g0008421 73.786 206 45 3 1 201 88 289 1.50e-104 302
Msa0669190 MtrunA17_Chr7g0266341 37.500 200 107 10 11 201 102 292 4.97e-25 99.0
Msa0669190 MtrunA17_Chr2g0306771 37.500 192 94 6 11 201 211 377 2.91e-22 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0669190 AT2G37970.1 60.648 216 69 3 1 201 32 246 7.21e-89 261
Msa0669190 AT5G20140.1 34.031 191 103 5 11 201 206 373 4.03e-21 90.1
Msa0669190 AT3G10130.1 36.683 199 110 9 11 201 115 305 3.08e-19 84.0
Msa0669190 AT5G20140.2 33.708 178 95 5 11 188 206 360 1.45e-18 82.8

Find 37 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACAAAGATGGTGGGTTTA+AGG 0.186605 4_4:+10757953 Msa0669190:CDS
TTTGATATGGGTTTAGTATT+TGG 0.199696 4_4:+10757810 None:intergenic
GAGGAAGGTGGGAAGAAATA+TGG 0.218011 4_4:+10758215 Msa0669190:CDS
AAATATGGGGTGATGACTTT+TGG 0.279249 4_4:+10758230 Msa0669190:CDS
GGAGAAGTTGAGGTTGAGTT+TGG 0.287349 4_4:+10758289 Msa0669190:CDS
TTCTTCTCATTTGTCACAAC+TGG 0.315475 4_4:-10758101 None:intergenic
TTGTCACCTTTGAATGTTGA+TGG 0.319242 4_4:-10757936 None:intergenic
CGATTACACTTCCAATGTTT+AGG 0.323933 4_4:+10758369 Msa0669190:CDS
AGGAAGGTGGGAAGAAATAT+GGG 0.347201 4_4:+10758216 Msa0669190:CDS
ATCATCATGGTTCATTCAAC+AGG 0.393338 4_4:-10758410 None:intergenic
TTTGAATGTTGATGGATCAT+AGG 0.426497 4_4:-10757928 None:intergenic
ATCACAACCCTCTCATCAAT+AGG 0.466452 4_4:-10758188 None:intergenic
TTGTGATAAGAGAGGAAGGT+GGG 0.471919 4_4:+10758204 Msa0669190:CDS
AGGGTTGTGATAAGAGAGGA+AGG 0.486753 4_4:+10758200 Msa0669190:CDS
AGGAGAAGGTGGAGAAGTTG+AGG 0.492065 4_4:+10758279 Msa0669190:CDS
AGGTTGAGTTTGGAGAAAGA+TGG 0.495669 4_4:+10758299 Msa0669190:CDS
TCCAATGTTTAGGACTAATG+AGG 0.518493 4_4:+10758379 Msa0669190:CDS
TGAGAGGGTTGTGATAAGAG+AGG 0.529346 4_4:+10758196 Msa0669190:CDS
TATGATCCATCAACATTCAA+AGG 0.542632 4_4:+10757930 Msa0669190:CDS
ACCTAAGCCTATTGATGAGA+GGG 0.548573 4_4:+10758181 Msa0669190:CDS
ACCCTCTCATCAATAGGCTT+AGG 0.549333 4_4:-10758182 None:intergenic
TGAAGTGGTGAAGGAGAAGG+TGG 0.561858 4_4:+10758268 Msa0669190:CDS
ACCTCATTAGTCCTAAACAT+TGG 0.572004 4_4:-10758380 None:intergenic
ACATTCAAAGGTGACAAAGA+TGG 0.580055 4_4:+10757942 Msa0669190:CDS
AACATTGGAAGTGTAATCGC+AGG 0.582795 4_4:-10758365 None:intergenic
GGCAAATGATGAAGTGGTGA+AGG 0.590348 4_4:+10758259 Msa0669190:CDS
TTCAAAGGTGACAAAGATGG+TGG 0.592548 4_4:+10757945 Msa0669190:CDS
GTGATTTCAGCTACTAGAGA+AGG 0.604224 4_4:-10757906 None:intergenic
TACCTAAGCCTATTGATGAG+AGG 0.615966 4_4:+10758180 Msa0669190:CDS
TGATGAAGTGGTGAAGGAGA+AGG 0.619951 4_4:+10758265 Msa0669190:CDS
ATTGGAAGTGTAATCGCAGG+TGG 0.628003 4_4:-10758362 None:intergenic
AGGAGTGGCAAATGATGAAG+TGG 0.629210 4_4:+10758253 Msa0669190:CDS
TCAAAGGTGACAAAGATGGT+GGG 0.643345 4_4:+10757946 Msa0669190:CDS
GTTGTGATAAGAGAGGAAGG+TGG 0.671478 4_4:+10758203 Msa0669190:CDS
GGAAGGTGGGAAGAAATATG+GGG 0.674694 4_4:+10758217 Msa0669190:CDS
GACAAATGAGAAGAACAAGA+TGG 0.687039 4_4:+10758109 Msa0669190:CDS
GACTTCACCAGTTATCACAA+AGG 0.689440 4_4:+10758040 Msa0669190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTGAGTATTTCATATTT+TGG - chr4_4:10757855-10757874 None:intergenic 20.0%
!!! AAGGTTTTAGTTGATTACAT+AGG + chr4_4:10757972-10757991 Msa0669190:CDS 25.0%
!! GTTGATTACATAGGAATTTT+TGG + chr4_4:10757981-10758000 Msa0669190:CDS 25.0%
!!! TTTTGTGTTTTTGTGTTTTG+AGG - chr4_4:10758011-10758030 None:intergenic 25.0%
!!! TCTACTTTTTCATACATAGA+AGG - chr4_4:10758155-10758174 None:intergenic 25.0%
! TTCTATGTATGAAAAAGTAG+AGG + chr4_4:10758154-10758173 Msa0669190:CDS 25.0%
!!! AAAGATGGTTTTAAGGTTAT+TGG + chr4_4:10758314-10758333 Msa0669190:CDS 25.0%
!!! GTTATTGGAGATTTTTTGTT+GGG + chr4_4:10758329-10758348 Msa0669190:CDS 25.0%
! TTTGAATGTTGATGGATCAT+AGG - chr4_4:10757931-10757950 None:intergenic 30.0%
TATGATCCATCAACATTCAA+AGG + chr4_4:10757930-10757949 Msa0669190:CDS 30.0%
! TTGTTTTCCTTTGTGATAAC+TGG - chr4_4:10758050-10758069 None:intergenic 30.0%
!! TTTGGAGAAAGATGGTTTTA+AGG + chr4_4:10758307-10758326 Msa0669190:CDS 30.0%
!!! GGTTATTGGAGATTTTTTGT+TGG + chr4_4:10758328-10758347 Msa0669190:CDS 30.0%
!!! TCGGATTACATAGTTTTGTG+TGG - chr4_4:10757877-10757896 None:intergenic 35.0%
!! CTAGAGAAGGTGCATATTTT+CGG - chr4_4:10757896-10757915 None:intergenic 35.0%
TTGTCACCTTTGAATGTTGA+TGG - chr4_4:10757939-10757958 None:intergenic 35.0%
ACATTCAAAGGTGACAAAGA+TGG + chr4_4:10757942-10757961 Msa0669190:CDS 35.0%
TTCTTCTCATTTGTCACAAC+TGG - chr4_4:10758104-10758123 None:intergenic 35.0%
GACAAATGAGAAGAACAAGA+TGG + chr4_4:10758109-10758128 Msa0669190:CDS 35.0%
! AAATATGGGGTGATGACTTT+TGG + chr4_4:10758230-10758249 Msa0669190:CDS 35.0%
!!! TTGGAGATTTTTTGTTGGGT+AGG + chr4_4:10758333-10758352 Msa0669190:CDS 35.0%
CGATTACACTTCCAATGTTT+AGG + chr4_4:10758369-10758388 Msa0669190:CDS 35.0%
! TCCAATGTTTAGGACTAATG+AGG + chr4_4:10758379-10758398 Msa0669190:CDS 35.0%
ACCTCATTAGTCCTAAACAT+TGG - chr4_4:10758383-10758402 None:intergenic 35.0%
GTGATTTCAGCTACTAGAGA+AGG - chr4_4:10757909-10757928 None:intergenic 40.0%
TTCAAAGGTGACAAAGATGG+TGG + chr4_4:10757945-10757964 Msa0669190:CDS 40.0%
TCAAAGGTGACAAAGATGGT+GGG + chr4_4:10757946-10757965 Msa0669190:CDS 40.0%
!! TGACAAAGATGGTGGGTTTA+AGG + chr4_4:10757953-10757972 Msa0669190:CDS 40.0%
GACTTCACCAGTTATCACAA+AGG + chr4_4:10758040-10758059 Msa0669190:CDS 40.0%
TACCTAAGCCTATTGATGAG+AGG + chr4_4:10758180-10758199 Msa0669190:CDS 40.0%
ACCTAAGCCTATTGATGAGA+GGG + chr4_4:10758181-10758200 Msa0669190:CDS 40.0%
ATCACAACCCTCTCATCAAT+AGG - chr4_4:10758191-10758210 None:intergenic 40.0%
TTGTGATAAGAGAGGAAGGT+GGG + chr4_4:10758204-10758223 Msa0669190:CDS 40.0%
AGGAAGGTGGGAAGAAATAT+GGG + chr4_4:10758216-10758235 Msa0669190:CDS 40.0%
AGGTTGAGTTTGGAGAAAGA+TGG + chr4_4:10758299-10758318 Msa0669190:CDS 40.0%
! AACATTGGAAGTGTAATCGC+AGG - chr4_4:10758368-10758387 None:intergenic 40.0%
ACCCTCTCATCAATAGGCTT+AGG - chr4_4:10758185-10758204 None:intergenic 45.0%
TGAGAGGGTTGTGATAAGAG+AGG + chr4_4:10758196-10758215 Msa0669190:CDS 45.0%
AGGGTTGTGATAAGAGAGGA+AGG + chr4_4:10758200-10758219 Msa0669190:CDS 45.0%
GTTGTGATAAGAGAGGAAGG+TGG + chr4_4:10758203-10758222 Msa0669190:CDS 45.0%
GAGGAAGGTGGGAAGAAATA+TGG + chr4_4:10758215-10758234 Msa0669190:CDS 45.0%
GGAAGGTGGGAAGAAATATG+GGG + chr4_4:10758217-10758236 Msa0669190:CDS 45.0%
! TATGGGGTGATGACTTTTGG+AGG + chr4_4:10758233-10758252 Msa0669190:CDS 45.0%
AGGAGTGGCAAATGATGAAG+TGG + chr4_4:10758253-10758272 Msa0669190:CDS 45.0%
GGCAAATGATGAAGTGGTGA+AGG + chr4_4:10758259-10758278 Msa0669190:CDS 45.0%
TGATGAAGTGGTGAAGGAGA+AGG + chr4_4:10758265-10758284 Msa0669190:CDS 45.0%
GGAGAAGTTGAGGTTGAGTT+TGG + chr4_4:10758289-10758308 Msa0669190:CDS 45.0%
! ATTGGAAGTGTAATCGCAGG+TGG - chr4_4:10758365-10758384 None:intergenic 45.0%
!! GGTGATGACTTTTGGAGGAG+TGG + chr4_4:10758238-10758257 Msa0669190:CDS 50.0%
TGAAGTGGTGAAGGAGAAGG+TGG + chr4_4:10758268-10758287 Msa0669190:CDS 50.0%
AGGAGAAGGTGGAGAAGTTG+AGG + chr4_4:10758279-10758298 Msa0669190:CDS 50.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 10757816 10758421 10757816 ID=Msa0669190;Name=Msa0669190
chr4_4 mRNA 10757816 10758421 10757816 ID=Msa0669190-mRNA-1;Parent=Msa0669190;Name=Msa0669190-mRNA-1;_AED=0.25;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|201
chr4_4 exon 10757816 10758421 10757816 ID=Msa0669190-mRNA-1:exon:3206;Parent=Msa0669190-mRNA-1
chr4_4 CDS 10757816 10758421 10757816 ID=Msa0669190-mRNA-1:cds;Parent=Msa0669190-mRNA-1
Gene Sequence

>Msa0669190

ATGGGTTTAGTATTTGGAAGATTTAGTGCTGAGACACCAAAATATGAAATACTCAAAACCACACAAAACTATGTAATCCGAAAATATGCACCTTCTCTAGTAGCTGAAATCACCTATGATCCATCAACATTCAAAGGTGACAAAGATGGTGGGTTTAAGGTTTTAGTTGATTACATAGGAATTTTTGGTAAACCTCAAAACACAAAAACACAAAAAATCTCAATGACTTCACCAGTTATCACAAAGGAAAACAAAAATTCTTCAGAAAAGATTGCTATGACAGTACCAGTTGTGACAAATGAGAAGAACAAGATGGTGACTATGCAGTTTACTTTGCCTTCTATGTATGAAAAAGTAGAGGAAGTACCTAAGCCTATTGATGAGAGGGTTGTGATAAGAGAGGAAGGTGGGAAGAAATATGGGGTGATGACTTTTGGAGGAGTGGCAAATGATGAAGTGGTGAAGGAGAAGGTGGAGAAGTTGAGGTTGAGTTTGGAGAAAGATGGTTTTAAGGTTATTGGAGATTTTTTGTTGGGTAGGTATAATCCACCTGCGATTACACTTCCAATGTTTAGGACTAATGAGGTTCTGATTCCTGTTGAATGA

Protein sequence

>Msa0669190

MGLVFGRFSAETPKYEILKTTQNYVIRKYAPSLVAEITYDPSTFKGDKDGGFKVLVDYIGIFGKPQNTKTQKISMTSPVITKENKNSSEKIAMTVPVVTNEKNKMVTMQFTLPSMYEKVEEVPKPIDERVVIREEGGKKYGVMTFGGVANDEVVKEKVEKLRLSLEKDGFKVIGDFLLGRYNPPAITLPMFRTNEVLIPVE*