AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0905380


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0905380 MtrunA17_Chr6g0453281 98.140 215 4 0 1 215 23 237 3.16e-158 437
Msa0905380 MtrunA17_Chr7g0217561 63.721 215 63 2 1 215 18 217 1.79e-86 254
Msa0905380 MtrunA17_Chr2g0287451 39.024 123 58 4 93 212 84 192 2.03e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0905380 AT3G01170.1 59.898 197 68 3 22 215 27 215 2.33e-78 234
Msa0905380 AT5G15260.2 60.753 186 62 5 38 215 52 234 8.13e-72 218
Msa0905380 AT5G15260.1 60.753 186 62 5 38 215 52 234 8.13e-72 218
Msa0905380 AT3G06180.1 40.000 165 94 3 53 215 66 227 4.27e-23 93.6
Msa0905380 AT5G19025.1 51.515 99 45 2 117 215 150 245 1.38e-22 92.4
Msa0905380 AT5G19025.2 50.526 95 45 1 117 211 150 242 2.60e-22 92.0
Msa0905380 AT3G01170.2 48.684 76 37 1 21 94 26 101 9.08e-17 74.7

Find 40 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTATATTTATCTATCTAT+TGG 0.242876 6_2:-3487445 Msa0905380:CDS
GTCATTTCAGATTATGAGTA+TGG 0.244984 6_2:-3487503 Msa0905380:CDS
TAACAGAATTTGATAAACTA+TGG 0.289659 6_2:+3487641 None:intergenic
TGTTGGTAGGTTAGAAGTTC+CGG 0.289963 6_2:-3487128 Msa0905380:CDS
AAGAAGATGGCACCGATTAA+TGG 0.325801 6_2:-3487190 Msa0905380:CDS
CCAATAGATAGATAAATATA+AGG 0.374350 6_2:+3487445 None:intergenic
ATGGCGAATTCGATCGAATT+CGG 0.383006 6_2:-3487718 Msa0905380:CDS
TTTGTGCTGCATTGGCTACA+TGG 0.387252 6_2:-3487411 Msa0905380:CDS
TTCTTCAAACCCTTACAATT+CGG 0.389737 6_2:+3487352 None:intergenic
CGAAGTTTCTTCGTCGGTCC+CGG 0.401109 6_2:+3487109 None:intergenic
GTTGGTAGGTTAGAAGTTCC+GGG 0.404512 6_2:-3487127 Msa0905380:CDS
ATCACTAAAAGTTCAACATA+AGG 0.412821 6_2:+3487529 None:intergenic
CTAATCAATTTCAAAGGAAT+AGG 0.420647 6_2:-3487669 Msa0905380:CDS
GATGGCGGTGTTTGTAAGAA+GGG 0.429289 6_2:-3487262 Msa0905380:CDS
AGCACAGCTCTTCCATTAAT+CGG 0.433317 6_2:+3487178 None:intergenic
ATTGCTGTTGTTATTGCTTG+TGG 0.436635 6_2:-3487565 Msa0905380:CDS
ATGCGGTAATCCGAATTGTA+AGG 0.440528 6_2:-3487362 Msa0905380:CDS
AGATGGCGGTGTTTGTAAGA+AGG 0.445969 6_2:-3487263 Msa0905380:CDS
CTGTGCTTGTGCTGAGAGCT+AGG 0.461752 6_2:-3487162 Msa0905380:CDS
GGTGTGGTTGTTCTGTTGGT+AGG 0.475981 6_2:-3487141 Msa0905380:CDS
GCTAGGTGTGGTTGTTCTGT+TGG 0.477365 6_2:-3487145 Msa0905380:CDS
ATCTTCCGAAGTTTCTTCGT+CGG 0.488611 6_2:+3487103 None:intergenic
TCACTAAAAGTTCAACATAA+GGG 0.493997 6_2:+3487530 None:intergenic
TTGTGCTGCATTGGCTACAT+GGG 0.507240 6_2:-3487410 Msa0905380:CDS
ATCAATTTCAAAGGAATAGG+AGG 0.510788 6_2:-3487666 Msa0905380:CDS
AAGTGTTGTTTGTGCTGCAT+TGG 0.523765 6_2:-3487419 Msa0905380:CDS
ACTTCGGAAGATCAAGAAGT+AGG 0.555452 6_2:-3487092 None:intergenic
AGCAAACTAATCAATTTCAA+AGG 0.564372 6_2:-3487675 Msa0905380:CDS
TGCGGTAATCCGAATTGTAA+GGG 0.568571 6_2:-3487361 Msa0905380:CDS
TGAAGCGGAGCTTAAGAAGA+TGG 0.571103 6_2:-3487203 Msa0905380:CDS
ATTGCCGCGTGATCATCATA+AGG 0.580739 6_2:-3487230 Msa0905380:CDS
CCGCCATCTTTCACAAAAGA+CGG 0.592301 6_2:+3487277 None:intergenic
TCATCATAAGGAACTTGAAG+CGG 0.636755 6_2:-3487218 Msa0905380:CDS
CGGGACCGACGAAGAAACTT+CGG 0.636873 6_2:-3487108 Msa0905380:CDS
TGTGCTGCATTGGCTACATG+GGG 0.654737 6_2:-3487409 Msa0905380:CDS
GTTTGCACAAGTAACAAATG+CGG 0.681482 6_2:-3487379 Msa0905380:CDS
CTTGTGCTGAGAGCTAGGTG+TGG 0.697858 6_2:-3487157 Msa0905380:CDS
GCTGATCTAGATCGATCACA+AGG 0.698983 6_2:+3487610 None:intergenic
GATCTAGATCGATCACAAGG+TGG 0.717259 6_2:+3487613 None:intergenic
AGTTCCTTATGATGATCACG+CGG 0.724998 6_2:+3487226 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAACAGAATTTGATAAACTA+TGG + chr6_2:3487173-3487192 None:intergenic 20.0%
!! CCAATAGATAGATAAATATA+AGG + chr6_2:3487369-3487388 None:intergenic 20.0%
!! CCTTATATTTATCTATCTAT+TGG - chr6_2:3487366-3487385 Msa0905380:CDS 20.0%
! AGCAAACTAATCAATTTCAA+AGG - chr6_2:3487136-3487155 Msa0905380:CDS 25.0%
! CTAATCAATTTCAAAGGAAT+AGG - chr6_2:3487142-3487161 Msa0905380:CDS 25.0%
! TCACTAAAAGTTCAACATAA+GGG + chr6_2:3487284-3487303 None:intergenic 25.0%
! ATCACTAAAAGTTCAACATA+AGG + chr6_2:3487285-3487304 None:intergenic 25.0%
ATCAATTTCAAAGGAATAGG+AGG - chr6_2:3487145-3487164 Msa0905380:CDS 30.0%
GTCATTTCAGATTATGAGTA+TGG - chr6_2:3487308-3487327 Msa0905380:CDS 30.0%
! TATGGTGTGTTTTCTGATTA+TGG - chr6_2:3487326-3487345 Msa0905380:CDS 30.0%
TTCTTCAAACCCTTACAATT+CGG + chr6_2:3487462-3487481 None:intergenic 30.0%
! ATTGCTGTTGTTATTGCTTG+TGG - chr6_2:3487246-3487265 Msa0905380:CDS 35.0%
GTTTGCACAAGTAACAAATG+CGG - chr6_2:3487432-3487451 Msa0905380:CDS 35.0%
TCATCATAAGGAACTTGAAG+CGG - chr6_2:3487593-3487612 Msa0905380:CDS 35.0%
ATGGCGAATTCGATCGAATT+CGG - chr6_2:3487093-3487112 Msa0905380:CDS 40.0%
!! AAGTGTTGTTTGTGCTGCAT+TGG - chr6_2:3487392-3487411 Msa0905380:CDS 40.0%
ATGCGGTAATCCGAATTGTA+AGG - chr6_2:3487449-3487468 Msa0905380:CDS 40.0%
TGCGGTAATCCGAATTGTAA+GGG - chr6_2:3487450-3487469 Msa0905380:CDS 40.0%
AGTTCCTTATGATGATCACG+CGG + chr6_2:3487588-3487607 None:intergenic 40.0%
!! AAGAAGATGGCACCGATTAA+TGG - chr6_2:3487621-3487640 Msa0905380:CDS 40.0%
AGCACAGCTCTTCCATTAAT+CGG + chr6_2:3487636-3487655 None:intergenic 40.0%
! TGTTGGTAGGTTAGAAGTTC+CGG - chr6_2:3487683-3487702 Msa0905380:CDS 40.0%
ATCTTCCGAAGTTTCTTCGT+CGG + chr6_2:3487711-3487730 None:intergenic 40.0%
GATCTAGATCGATCACAAGG+TGG + chr6_2:3487201-3487220 None:intergenic 45.0%
GCTGATCTAGATCGATCACA+AGG + chr6_2:3487204-3487223 None:intergenic 45.0%
!! TTTGTGCTGCATTGGCTACA+TGG - chr6_2:3487400-3487419 Msa0905380:CDS 45.0%
!! TTGTGCTGCATTGGCTACAT+GGG - chr6_2:3487401-3487420 Msa0905380:CDS 45.0%
! TCGCCGTCTTTTGTGAAAGA+TGG - chr6_2:3487531-3487550 Msa0905380:CDS 45.0%
CCGCCATCTTTCACAAAAGA+CGG + chr6_2:3487537-3487556 None:intergenic 45.0%
! CCGTCTTTTGTGAAAGATGG+CGG - chr6_2:3487534-3487553 Msa0905380:CDS 45.0%
! AGATGGCGGTGTTTGTAAGA+AGG - chr6_2:3487548-3487567 Msa0905380:CDS 45.0%
! GATGGCGGTGTTTGTAAGAA+GGG - chr6_2:3487549-3487568 Msa0905380:CDS 45.0%
ATTGCCGCGTGATCATCATA+AGG - chr6_2:3487581-3487600 Msa0905380:CDS 45.0%
TGAAGCGGAGCTTAAGAAGA+TGG - chr6_2:3487608-3487627 Msa0905380:CDS 45.0%
! GTTGGTAGGTTAGAAGTTCC+GGG - chr6_2:3487684-3487703 Msa0905380:CDS 45.0%
!! TGTGCTGCATTGGCTACATG+GGG - chr6_2:3487402-3487421 Msa0905380:CDS 50.0%
GCTAGGTGTGGTTGTTCTGT+TGG - chr6_2:3487666-3487685 Msa0905380:CDS 50.0%
GGTGTGGTTGTTCTGTTGGT+AGG - chr6_2:3487670-3487689 Msa0905380:CDS 50.0%
CTGTGCTTGTGCTGAGAGCT+AGG - chr6_2:3487649-3487668 Msa0905380:CDS 55.0%
CTTGTGCTGAGAGCTAGGTG+TGG - chr6_2:3487654-3487673 Msa0905380:CDS 55.0%
CGAAGTTTCTTCGTCGGTCC+CGG + chr6_2:3487705-3487724 None:intergenic 55.0%
CGGGACCGACGAAGAAACTT+CGG - chr6_2:3487703-3487722 Msa0905380:CDS 55.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 3487093 3487740 3487093 ID=Msa0905380;Name=Msa0905380
chr6_2 mRNA 3487093 3487740 3487093 ID=Msa0905380-mRNA-1;Parent=Msa0905380;Name=Msa0905380-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|215
chr6_2 exon 3487093 3487740 3487093 ID=Msa0905380-mRNA-1:exon:1047;Parent=Msa0905380-mRNA-1
chr6_2 CDS 3487093 3487740 3487093 ID=Msa0905380-mRNA-1:cds;Parent=Msa0905380-mRNA-1
Gene Sequence

>Msa0905380

ATGGCGAATTCGATCGAATTCGGATCGAAATATTCAAAATCTAAGCAAACTAATCAATTTCAAAGGAATAGGAGGAACCATAGTTTATCAAATTCTGTTAAGATTCCACCTTGTGATCGATCTAGATCAGCTATGATTGATGTTGTTATGTTCATTGCTGTTGTTATTGCTTGTGGATTTTTGTTTTTCCCTTATGTTGAACTTTTAGTGATTAAGTCATTTCAGATTATGAGTATGGTGTGTTTTCTGATTATGGAAGAAGTTTCTTATGCACCTTATATTTATCTATCTATTGGAATAAGTGTTGTTTGTGCTGCATTGGCTACATGGGGTGTTATTGTTTGCACAAGTAACAAATGCGGTAATCCGAATTGTAAGGGTTTGAAGAAAGCTGCAGAATTTGATATTCAGTTAGAGACTGAAGATTGTGTGAAGAATTCGCCGTCTTTTGTGAAAGATGGCGGTGTTTGTAAGAAGGGTTTGTTTGAATTGCCGCGTGATCATCATAAGGAACTTGAAGCGGAGCTTAAGAAGATGGCACCGATTAATGGAAGAGCTGTGCTTGTGCTGAGAGCTAGGTGTGGTTGTTCTGTTGGTAGGTTAGAAGTTCCGGGACCGACGAAGAAACTTCGGAAGATCAAGAAGTAG

Protein sequence

>Msa0905380

MANSIEFGSKYSKSKQTNQFQRNRRNHSLSNSVKIPPCDRSRSAMIDVVMFIAVVIACGFLFFPYVELLVIKSFQIMSMVCFLIMEEVSYAPYIYLSIGISVVCAALATWGVIVCTSNKCGNPNCKGLKKAAEFDIQLETEDCVKNSPSFVKDGGVCKKGLFELPRDHHKELEAELKKMAPINGRAVLVLRARCGCSVGRLEVPGPTKKLRKIKK*