AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0926080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0926080 MtrunA17_Chr6g0487261 98.225 507 9 0 1 507 1 507 0.0 1028
Msa0926080 MtrunA17_Chr8g0389741 79.324 503 97 5 5 507 7 502 0.0 815
Msa0926080 MtrunA17_Chr3g0110371 40.509 432 245 3 26 454 23 445 9.65e-120 360
Msa0926080 MtrunA17_Chr4g0034901 39.294 453 268 4 7 454 9 459 1.89e-119 358
Msa0926080 MtrunA17_Chr1g0161011 39.836 487 275 5 9 486 1 478 2.28e-118 357
Msa0926080 MtrunA17_Chr8g0378621 37.600 500 296 5 11 502 7 498 2.28e-118 357
Msa0926080 MtrunA17_Chr5g0439251 40.816 441 245 3 23 448 21 460 4.11e-118 356
Msa0926080 MtrunA17_Chr4g0075031 39.545 483 273 6 24 501 42 510 1.25e-117 356
Msa0926080 MtrunA17_Chr1g0177171 39.514 453 245 4 27 452 38 488 3.57e-117 354
Msa0926080 MtrunA17_Chr5g0434591 39.741 463 264 6 7 465 5 456 8.16e-117 353
Msa0926080 MtrunA17_Chr3g0144531 39.035 456 273 3 5 459 8 459 9.10e-117 352
Msa0926080 MtrunA17_Chr3g0121271 34.851 505 300 8 11 499 7 498 1.33e-113 345
Msa0926080 MtrunA17_Chr7g0234821 40.783 434 248 8 28 454 27 458 3.15e-112 340
Msa0926080 MtrunA17_Chr8g0339191 39.815 432 252 7 28 454 30 458 1.06e-110 336
Msa0926080 MtrunA17_Chr7g0223961 40.092 434 242 11 27 452 23 446 3.76e-102 313
Msa0926080 MtrunA17_Chr4g0046311 36.444 450 274 7 9 448 4 451 1.09e-98 305
Msa0926080 MtrunA17_Chr4g0043511 37.022 497 282 14 27 501 62 549 2.84e-96 302
Msa0926080 MtrunA17_Chr3g0119541 42.727 330 188 1 21 349 20 349 4.48e-93 287
Msa0926080 MtrunA17_Chr4g0026321 43.844 333 179 3 22 350 24 352 1.69e-92 286
Msa0926080 MtrunA17_Chr1g0189111 45.483 321 172 3 27 346 31 349 1.87e-92 288
Msa0926080 MtrunA17_Chr4g0003281 39.516 372 222 2 5 375 18 387 2.13e-91 284
Msa0926080 MtrunA17_Chr4g0043851 42.382 361 199 3 28 387 33 385 1.02e-90 283
Msa0926080 MtrunA17_Chr2g0321511 41.092 348 198 3 7 350 6 350 2.09e-89 279
Msa0926080 MtrunA17_Chr1g0155001 41.509 318 184 2 26 341 23 340 2.26e-85 269
Msa0926080 MtrunA17_Chr1g0146831 42.900 331 181 5 14 341 18 343 1.04e-83 264
Msa0926080 MtrunA17_Chr2g0285901 41.716 338 194 3 7 343 4 339 1.46e-82 261
Msa0926080 MtrunA17_Chr4g0064021 40.606 330 194 2 19 347 19 347 2.14e-82 261
Msa0926080 MtrunA17_Chr2g0294601 37.243 341 199 8 27 362 59 389 1.41e-71 233
Msa0926080 MtrunA17_Chr4g0038941 36.697 327 194 6 19 345 44 357 1.93e-71 231
Msa0926080 MtrunA17_Chr2g0294591 37.538 325 191 7 27 348 30 345 2.55e-71 231
Msa0926080 MtrunA17_Chr8g0383311 31.838 468 283 15 7 454 15 466 1.02e-70 234
Msa0926080 MtrunA17_Chr4g0039071 37.299 311 187 4 27 336 25 328 4.50e-69 226
Msa0926080 MtrunA17_Chr7g0250301 35.016 317 198 5 27 343 8 316 6.52e-69 223
Msa0926080 MtrunA17_Chr4g0039021 35.986 289 177 2 47 335 1 281 1.34e-67 219
Msa0926080 MtrunA17_Chr4g0038981 35.737 319 192 6 27 345 23 328 3.25e-67 219
Msa0926080 MtrunA17_Chr4g0039011 34.675 323 199 5 25 347 40 350 3.53e-67 220
Msa0926080 MtrunA17_Chr1g0174261 38.652 282 165 4 56 336 1 275 3.27e-64 212
Msa0926080 MtrunA17_Chr4g0038961 33.136 338 213 6 8 345 4 328 3.98e-64 211
Msa0926080 MtrunA17_Chr4g0038971 33.333 339 213 6 7 345 3 328 1.67e-63 209
Msa0926080 MtrunA17_Chr3g0138741 31.878 458 283 16 7 454 16 454 4.03e-63 213
Msa0926080 MtrunA17_Chr1g0148391 31.567 453 283 14 14 454 11 448 4.04e-63 213
Msa0926080 MtrunA17_Chr3g0129341 30.149 471 296 18 1 454 1 455 1.58e-60 206
Msa0926080 MtrunA17_Chr4g0062771 31.223 458 285 17 8 452 14 454 2.38e-60 206
Msa0926080 MtrunA17_Chr4g0076581 30.591 474 304 13 14 474 15 476 4.11e-60 205
Msa0926080 MtrunA17_Chr2g0306311 28.542 480 295 13 9 452 12 479 1.87e-58 201
Msa0926080 MtrunA17_Chr2g0290821 30.068 439 286 12 25 455 26 451 7.18e-58 199
Msa0926080 MtrunA17_Chr3g0105981 31.163 430 251 16 27 438 27 429 2.75e-56 194
Msa0926080 MtrunA17_Chr7g0253091 31.236 461 279 18 6 451 5 442 5.22e-56 194
Msa0926080 MtrunA17_Chr6g0461601 29.787 470 290 15 6 455 5 454 1.12e-55 193
Msa0926080 MtrunA17_Chr5g0436361 35.806 310 185 10 43 346 53 354 3.31e-51 179
Msa0926080 MtrunA17_Chr4g0069801 29.295 454 280 19 40 476 41 470 6.55e-51 181
Msa0926080 MtrunA17_Chr7g0250321 33.083 266 171 4 27 292 28 286 9.05e-51 175
Msa0926080 MtrunA17_Chr8g0381081 56.842 95 40 1 365 459 53 146 4.35e-34 126
Msa0926080 MtrunA17_Chr2g0294581 34.667 150 89 2 27 174 76 218 8.16e-24 101
Msa0926080 MtrunA17_Chr2g0294581 48.421 95 41 5 243 336 187 274 6.18e-15 75.5
Msa0926080 MtrunA17_Chr1g0213651 48.276 87 43 1 369 455 276 360 2.66e-23 101
Msa0926080 MtrunA17_Chr3g0096451 45.192 104 55 2 351 454 94 195 3.42e-23 99.0
Msa0926080 MtrunA17_Chr5g0447651 47.674 86 44 1 369 454 30 114 4.07e-23 94.4
Msa0926080 MtrunA17_Chr5g0443331 34.932 146 85 4 364 501 103 246 1.40e-21 94.4
Msa0926080 MtrunA17_Chr8g0378381 48.837 86 42 1 369 454 32 115 3.65e-21 89.0
Msa0926080 MtrunA17_Chr8g0378361 38.983 118 65 2 368 480 29 144 3.42e-20 88.6
Msa0926080 MtrunA17_Chr3g0120401 42.697 89 49 1 369 457 157 243 8.31e-20 89.0
Msa0926080 MtrunA17_Chr8g0378401 47.674 86 43 1 369 454 32 115 2.26e-19 84.0
Msa0926080 MtrunA17_Chr4g0040911 35.507 138 82 3 369 506 37 167 9.50e-19 84.0
Msa0926080 MtrunA17_Chr1g0154991 40.217 92 51 2 369 460 20 107 5.00e-18 79.7
Msa0926080 MtrunA17_Chr8g0378371 44.186 86 43 2 369 454 33 113 5.80e-18 79.7
Msa0926080 MtrunA17_Chr5g0401301 43.182 88 48 2 369 455 329 415 8.65e-18 85.5
Msa0926080 MtrunA17_Chr2g0304061 42.391 92 50 2 368 459 157 245 9.01e-18 84.3
Msa0926080 MtrunA17_Chr1g0193131 41.304 92 51 2 357 448 408 496 4.26e-17 84.3
Msa0926080 MtrunA17_Chr2g0313891 41.758 91 49 2 365 454 25 112 8.67e-17 76.3
Msa0926080 MtrunA17_Chr4g0076131 37.931 87 53 1 368 454 27 112 9.77e-17 76.3
Msa0926080 MtrunA17_Chr7g0271411 41.860 86 48 2 369 454 160 243 1.35e-16 80.5
Msa0926080 MtrunA17_Chr4g0018771 43.038 79 40 2 258 336 95 168 1.77e-15 75.9
Msa0926080 MtrunA17_Chr4g0061401 38.372 86 50 2 369 454 24 106 3.62e-15 74.7
Msa0926080 MtrunA17_Chr4g0018861 43.038 79 40 2 258 336 93 166 4.38e-15 74.3
Msa0926080 MtrunA17_Chr4g0076141 40.741 81 47 1 368 448 12 91 2.24e-14 68.9
Msa0926080 MtrunA17_Chr5g0419121 38.824 85 50 1 364 448 17 99 7.39e-14 71.6
Msa0926080 MtrunA17_Chr8g0380231 44.444 72 38 2 377 448 25 94 1.25e-13 67.0
Msa0926080 MtrunA17_Chr4g0043151 36.782 87 52 2 369 454 21 105 1.65e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0926080 AT5G56590.1 70.652 460 131 4 20 477 19 476 0.0 681
Msa0926080 AT4G29360.2 62.030 532 164 9 5 499 9 539 0.0 647
Msa0926080 AT4G29360.1 62.167 526 163 9 5 499 9 529 0.0 643
Msa0926080 AT2G01630.1 42.609 460 250 5 25 480 21 470 9.14e-128 382
Msa0926080 AT4G26830.1 42.369 439 249 4 15 452 14 449 1.83e-126 377
Msa0926080 AT4G26830.2 42.757 428 242 3 26 452 28 453 4.75e-126 376
Msa0926080 AT2G01630.3 43.560 427 229 4 25 448 21 438 1.40e-124 371
Msa0926080 AT5G55180.1 41.898 432 247 4 26 454 26 456 1.49e-123 369
Msa0926080 AT1G66250.1 40.741 486 273 6 24 504 29 504 7.86e-122 367
Msa0926080 AT5G55180.2 41.628 430 247 4 22 448 22 450 2.05e-121 364
Msa0926080 AT3G13560.4 40.000 465 261 6 6 466 6 456 2.91e-121 365
Msa0926080 AT3G13560.1 40.000 465 261 6 6 466 6 456 2.91e-121 365
Msa0926080 AT3G13560.2 40.000 465 261 6 6 466 6 456 2.91e-121 365
Msa0926080 AT3G13560.5 40.000 465 261 6 6 466 6 456 2.91e-121 365
Msa0926080 AT3G13560.3 40.000 465 261 6 6 466 6 456 2.91e-121 365
Msa0926080 AT2G16230.1 37.787 479 292 4 25 502 23 496 2.42e-119 360
Msa0926080 AT2G05790.1 40.225 445 248 5 27 454 25 468 2.70e-119 359
Msa0926080 AT4G34480.1 41.860 430 242 3 26 454 25 447 3.79e-118 357
Msa0926080 AT1G11820.2 39.004 482 277 6 26 504 43 510 4.46e-116 352
Msa0926080 AT1G11820.3 41.315 426 238 4 26 448 43 459 9.57e-113 342
Msa0926080 AT3G07320.1 38.194 432 260 6 28 454 26 455 1.37e-103 318
Msa0926080 AT2G01630.2 44.000 375 198 4 25 396 21 386 1.99e-103 315
Msa0926080 AT5G24318.1 36.819 459 270 9 6 454 7 455 1.31e-102 315
Msa0926080 AT4G14080.1 36.902 439 265 7 24 454 39 473 1.72e-100 311
Msa0926080 AT3G23770.1 38.041 439 261 6 25 454 35 471 3.36e-100 310
Msa0926080 AT5G24318.2 36.184 456 271 9 6 451 7 452 2.27e-99 307
Msa0926080 AT5G42720.1 43.895 344 186 3 10 346 4 347 3.85e-98 303
Msa0926080 AT2G27500.2 43.266 349 196 2 20 367 22 369 2.88e-96 296
Msa0926080 AT2G27500.1 43.732 343 191 2 20 361 22 363 7.22e-96 296
Msa0926080 AT1G32860.1 45.031 322 173 3 27 345 29 349 1.18e-94 294
Msa0926080 AT4G18340.2 44.606 343 186 3 27 368 34 373 1.73e-94 292
Msa0926080 AT4G18340.1 44.606 343 186 3 27 368 34 373 2.24e-94 292
Msa0926080 AT3G55430.1 37.615 436 253 14 25 454 27 449 4.34e-94 293
Msa0926080 AT1G30080.2 47.188 320 167 2 25 343 34 352 1.33e-93 290
Msa0926080 AT1G30080.1 47.188 320 167 2 25 343 34 352 3.72e-93 290
Msa0926080 AT4G34480.3 42.815 341 187 3 115 454 3 336 5.73e-91 283
Msa0926080 AT2G26600.1 43.293 328 182 2 22 345 26 353 1.05e-89 280
Msa0926080 AT3G15800.1 42.857 322 182 2 28 347 44 365 1.53e-86 272
Msa0926080 AT4G34480.2 43.260 319 180 1 26 343 25 343 5.18e-86 269
Msa0926080 AT1G11820.1 42.202 327 186 1 26 349 43 369 9.49e-86 270
Msa0926080 AT2G39640.1 35.045 448 270 10 7 452 12 440 9.46e-84 270
Msa0926080 AT2G39640.1 29.508 122 72 2 344 453 423 542 2.05e-14 76.3
Msa0926080 AT3G61810.1 42.025 326 175 4 27 346 55 372 1.15e-81 259
Msa0926080 AT5G24318.4 39.359 343 198 6 6 343 7 344 1.44e-81 258
Msa0926080 AT5G42100.2 41.796 323 183 4 25 343 25 346 4.43e-81 259
Msa0926080 AT5G42100.1 41.796 323 183 4 25 343 25 346 5.56e-81 258
Msa0926080 AT2G27500.3 44.755 286 156 2 77 361 1 285 1.35e-80 254
Msa0926080 AT4G16260.1 38.416 341 200 6 7 346 4 335 7.40e-78 248
Msa0926080 AT5G24318.3 38.462 325 185 5 136 454 1 316 1.55e-74 238
Msa0926080 AT3G57270.1 37.346 324 193 6 24 345 24 339 2.90e-73 236
Msa0926080 AT4G16260.2 38.978 313 181 6 35 346 3 306 3.37e-71 229
Msa0926080 AT3G57260.1 35.174 344 204 7 9 345 7 338 2.00e-70 228
Msa0926080 AT3G57260.2 35.174 344 204 7 9 345 23 354 4.67e-70 228
Msa0926080 AT3G57240.1 37.304 319 187 7 27 345 35 340 1.05e-68 224
Msa0926080 AT2G26600.2 43.191 257 142 2 93 345 3 259 2.26e-68 221
Msa0926080 AT3G46570.1 39.286 336 191 9 14 343 14 342 2.11e-67 221
Msa0926080 AT5G20390.1 36.418 335 197 8 17 343 17 343 5.63e-64 212
Msa0926080 AT5G20340.1 35.737 319 195 7 27 343 43 353 1.60e-61 206
Msa0926080 AT5G18220.1 30.691 492 291 17 25 500 28 485 3.86e-61 208
Msa0926080 AT3G24330.1 31.522 460 278 17 23 463 35 476 8.50e-61 208
Msa0926080 AT5G58090.1 31.081 444 282 13 21 454 16 445 1.49e-60 206
Msa0926080 AT3G04010.1 30.691 492 295 16 25 502 31 490 8.31e-60 205
Msa0926080 AT1G77780.1 34.648 355 217 7 25 379 22 361 1.22e-59 201
Msa0926080 AT5G20330.1 34.294 347 209 9 5 343 9 344 1.62e-59 200
Msa0926080 AT4G31140.1 30.137 438 280 13 27 454 27 448 6.14e-59 202
Msa0926080 AT1G33220.1 35.354 297 184 5 27 322 34 323 2.93e-58 196
Msa0926080 AT4G17180.1 30.932 472 284 16 9 465 7 451 6.54e-57 197
Msa0926080 AT1G77790.1 32.845 341 220 6 5 343 8 341 7.02e-56 191
Msa0926080 AT5G64790.1 29.130 460 297 15 8 454 8 451 7.54e-56 194
Msa0926080 AT5G58480.1 30.296 439 275 12 27 454 26 444 1.26e-55 193
Msa0926080 AT5G20870.1 29.328 491 301 17 31 500 34 499 1.09e-53 189
Msa0926080 AT2G19440.2 30.000 460 285 16 7 452 6 442 3.71e-53 187
Msa0926080 AT2G19440.1 30.000 460 285 16 7 452 6 442 3.71e-53 187
Msa0926080 AT3G55780.1 34.177 316 198 7 41 351 49 359 9.63e-52 181
Msa0926080 AT1G64760.1 30.227 440 275 16 27 452 25 446 2.23e-50 179
Msa0926080 AT1G64760.2 30.227 440 275 16 27 452 25 446 2.23e-50 179
Msa0926080 AT5G20560.1 32.441 299 195 5 25 322 32 324 2.90e-46 164
Msa0926080 AT4G05430.1 52.222 90 42 1 365 454 19 107 9.97e-29 111
Msa0926080 AT4G05430.3 52.222 90 42 1 365 454 44 132 1.31e-28 111
Msa0926080 AT5G67460.1 27.027 370 206 11 94 455 65 378 1.53e-28 117
Msa0926080 AT4G05430.2 53.012 83 38 1 366 448 45 126 2.12e-26 104
Msa0926080 AT5G67460.2 27.072 362 200 11 95 448 66 371 9.87e-26 108
Msa0926080 AT1G66870.1 39.344 122 62 2 333 454 1 110 2.38e-25 100
Msa0926080 AT1G66870.2 48.837 86 43 1 369 454 53 137 6.83e-25 100
Msa0926080 AT4G16165.1 36.066 122 66 2 333 454 1 110 3.75e-23 94.7
Msa0926080 AT5G63225.1 51.724 87 40 1 368 454 24 108 1.86e-22 92.8
Msa0926080 AT1G66852.1 45.349 86 45 2 369 454 26 109 2.07e-22 92.4
Msa0926080 AT1G66855.1 45.349 86 46 1 369 454 26 110 4.11e-22 91.7
Msa0926080 AT4G09462.1 50.000 86 41 1 369 454 29 112 5.03e-22 91.7
Msa0926080 AT4G09465.1 48.837 86 42 1 369 454 31 114 1.08e-21 90.9
Msa0926080 AT4G09090.1 46.067 89 46 1 367 455 29 115 1.30e-21 90.5
Msa0926080 AT4G09464.1 48.837 86 42 1 369 454 31 114 1.35e-21 90.5
Msa0926080 AT4G09467.1 48.837 86 42 1 369 454 31 114 1.35e-21 90.5
Msa0926080 AT2G30933.1 36.765 136 80 4 369 501 82 214 7.87e-21 91.7
Msa0926080 AT5G63240.1 41.414 99 57 1 358 455 29 127 7.88e-21 89.0
Msa0926080 AT1G79480.1 42.391 92 51 1 363 454 305 394 7.92e-21 95.1
Msa0926080 AT1G79480.2 42.391 92 51 1 363 454 304 393 8.07e-21 95.1
Msa0926080 AT5G63250.1 42.424 99 56 1 358 455 31 129 8.88e-21 88.6
Msa0926080 AT4G09466.1 46.512 86 44 1 369 454 31 114 3.80e-20 86.3
Msa0926080 AT4G13600.1 36.607 112 66 2 369 480 61 167 1.60e-19 88.2
Msa0926080 AT1G09460.1 45.745 94 46 3 366 459 134 222 5.61e-19 88.6
Msa0926080 AT1G29380.1 45.977 87 45 2 368 454 147 231 1.11e-18 87.4
Msa0926080 AT5G35740.1 40.426 94 54 2 362 454 23 115 1.31e-18 82.0
Msa0926080 AT1G29380.3 45.977 87 45 2 368 454 147 231 1.62e-18 85.9
Msa0926080 AT2G04910.2 40.230 87 51 1 368 454 14 99 2.38e-18 80.9
Msa0926080 AT3G58100.1 35.833 120 75 2 369 487 41 159 5.31e-18 82.4
Msa0926080 AT1G29380.4 48.148 81 40 2 368 448 147 225 6.02e-18 83.6
Msa0926080 AT1G29380.2 48.148 81 40 2 368 448 147 225 6.02e-18 83.6
Msa0926080 AT1G78520.1 36.275 102 62 1 353 454 16 114 1.04e-17 79.3
Msa0926080 AT3G58100.2 35.833 120 75 2 369 487 117 235 1.41e-17 83.2
Msa0926080 AT5G53600.1 41.860 86 47 1 369 454 29 111 1.57e-17 79.0
Msa0926080 AT2G29735.1 39.362 94 53 2 363 454 25 116 2.09e-17 78.6
Msa0926080 AT5G63230.1 42.529 87 48 1 368 454 25 109 3.42e-17 77.8
Msa0926080 AT2G30933.2 43.750 80 43 2 369 448 82 159 4.62e-17 79.0
Msa0926080 AT2G30933.3 43.750 80 43 2 369 448 82 159 4.62e-17 79.0
Msa0926080 AT5G53610.1 45.238 84 43 1 369 452 28 108 7.27e-17 77.0
Msa0926080 AT5G61130.1 38.372 86 51 1 369 454 21 104 1.22e-16 79.0
Msa0926080 AT1G18650.1 37.500 96 57 2 359 454 12 104 2.08e-16 77.8
Msa0926080 AT2G43670.1 36.842 95 57 1 360 454 26 117 2.38e-16 75.9
Msa0926080 AT2G04910.1 40.741 81 47 1 368 448 14 93 2.53e-16 75.9
Msa0926080 AT1G18650.2 37.500 96 57 2 359 454 12 104 4.60e-16 75.1
Msa0926080 AT5G08000.2 30.833 120 81 1 366 485 37 154 5.77e-14 71.6
Msa0926080 AT5G08000.1 36.047 86 53 1 369 454 21 104 4.56e-13 68.6
Msa0926080 AT1G26450.1 41.176 68 38 1 375 442 26 91 1.03e-11 64.7
Msa0926080 AT1G69295.1 37.209 86 50 3 369 453 21 103 7.80e-11 62.4

Find 119 sgRNAs with CRISPR-Local

Find 237 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTACAAAATGCGATAAAT+TGG 0.140800 6_2:-69648118 Msa0926080:CDS
AAAATGTTCATCCTGCTATT+TGG 0.231522 6_2:+69647106 None:intergenic
TCTGATGTTGCCTGTAGTTT+TGG 0.236535 6_2:-69647969 Msa0926080:CDS
TTTCGCTGTTCAACGAAAAT+AGG 0.254836 6_2:-69648319 Msa0926080:CDS
AATTGGGCTTGTGGTACTTC+TGG 0.267372 6_2:-69648101 Msa0926080:CDS
CTCTCCAACCAAAGTGTCTT+TGG 0.273000 6_2:-69648633 Msa0926080:CDS
AACTGCATCTACATGAAAAC+TGG 0.275506 6_2:-69647624 Msa0926080:CDS
AGCTCTTCTCCCGGCTTTGC+AGG 0.293675 6_2:+69648356 None:intergenic
GTCCGATATCCAAAAGAAAA+AGG 0.308137 6_2:+69647359 None:intergenic
GAACAATGGCAAGAGGGTTT+TGG 0.317261 6_2:-69650562 Msa0926080:exon
AATAGCTATTACCAGCAAAA+TGG 0.320368 6_2:-69647996 Msa0926080:CDS
CGAAAATAGGAAGCCGGGTT+TGG 0.322982 6_2:-69648306 Msa0926080:CDS
GGATATAGATCGATCATAAA+AGG 0.329265 6_2:+69648674 None:intergenic
ATTTGGTACTGCAGACCTTT+TGG 0.331387 6_2:+69646939 None:intergenic
TGTCATCGATCAAACTTGTT+AGG 0.331612 6_2:+69647333 None:intergenic
TGTTCTTGTTCTTGCTTGCT+TGG 0.340827 6_2:+69650630 None:intergenic
GTTTGGAATCTGAGAGGAAC+TGG 0.344642 6_2:-69648289 Msa0926080:CDS
TATTATTACTTGCCAAAGTT+TGG 0.347462 6_2:+69647473 None:intergenic
GTTGAGGTGTATGCTGCTTT+TGG 0.351356 6_2:-69647259 Msa0926080:three_prime_UTR
TTATACGTTTGTGCATTATC+AGG 0.352991 6_2:+69648419 None:intergenic
GCCAAGTGTCTGCATTAGTT+TGG 0.355290 6_2:+69648984 None:intergenic
CAAGCTTTCGATTGTTGAAA+AGG 0.360788 6_2:-69647305 Msa0926080:three_prime_UTR
ACCAACTGTGACACCTTATC+AGG 0.370353 6_2:+69649136 None:intergenic
AATGGAAGCAAATCTAGATT+TGG 0.371343 6_2:+69649016 None:intergenic
CTAGGCTGTACACGTTTGTT+AGG 0.371470 6_2:+69648249 None:intergenic
CAACTTCAAATGGAACAAAA+TGG 0.373327 6_2:-69648172 Msa0926080:CDS
GTATAAAGCAAACCAGTGTT+AGG 0.380170 6_2:+69648572 None:intergenic
CTTACAAAATGCGATAAATT+GGG 0.385650 6_2:-69648117 Msa0926080:CDS
AAAGTCATGGTAACCGAAAC+AGG 0.390886 6_2:-69648482 Msa0926080:CDS
CATGTAGATGCAGTTGTCAT+AGG 0.410440 6_2:+69647632 None:intergenic
GCTACTAGTTCAACTTCAAA+TGG 0.417514 6_2:-69648182 Msa0926080:CDS
GAAATTCCAACAGTGGCTTC+AGG 0.418186 6_2:+69648714 None:intergenic
GCATCTGCGGTCATGTCAAC+AGG 0.422571 6_2:+69648209 None:intergenic
TTCGGGACTTTCTGTAACTT+CGG 0.431562 6_2:+69648901 None:intergenic
GATAACATGTCTTATCAGAT+TGG 0.437839 6_2:+69648394 None:intergenic
GACTGGTGTACTTGTTGATA+AGG 0.445488 6_2:-69647943 Msa0926080:CDS
CTGTACACGTTTGTTAGGTC+CGG 0.458800 6_2:+69648254 None:intergenic
AACCGGTACACCTGCAAAGC+CGG 0.461559 6_2:-69648366 Msa0926080:CDS
GTGCAGAACTAACCAAACTT+TGG 0.474451 6_2:-69647485 Msa0926080:intron
CAGTCTCATTAGTTTCATGT+AGG 0.480668 6_2:-69647028 Msa0926080:three_prime_UTR
AACGCCAAGCGAATGAGTAC+TGG 0.481380 6_2:+69648793 None:intergenic
GACAGTTATGTATGTGATCT+TGG 0.481677 6_2:+69648928 None:intergenic
ACCGGCGTCGAACTTATGAT+TGG 0.486069 6_2:-69649043 Msa0926080:CDS
TGACTTGCCTCCACCTGATA+AGG 0.493506 6_2:-69649149 Msa0926080:CDS
TTAGTTTGGAACTGAGAGAA+TGG 0.494432 6_2:+69648998 None:intergenic
AGACATGTTATCAATGAAAC+CGG 0.498567 6_2:-69648383 Msa0926080:CDS
ATCTTGGTGGCTGGATAGTA+AGG 0.498877 6_2:+69648944 None:intergenic
GACCTCGTCCAGTAAAATCT+AGG 0.500092 6_2:+69648231 None:intergenic
ACTTCTTTGCTTGAGGAGGA+TGG 0.501780 6_2:+69647424 None:intergenic
TTCAACGAAAATAGGAAGCC+GGG 0.503312 6_2:-69648311 Msa0926080:CDS
CGATCGTTCCCTCCTTCCGC+CGG 0.503386 6_2:-69648764 Msa0926080:CDS
TGCATAGTCCAAAGACACTT+TGG 0.504638 6_2:+69648625 None:intergenic
TTCCTGTGTAGAGTCTAGAA+TGG 0.505000 6_2:+69650685 None:intergenic
AGTCTCATTAGTTTCATGTA+GGG 0.505475 6_2:-69647027 Msa0926080:three_prime_UTR
TTCCTCTCAGATTCCAAACC+CGG 0.507484 6_2:+69648293 None:intergenic
GAACTGGGGAATAGTTTATC+CGG 0.510770 6_2:-69648273 Msa0926080:CDS
TTTCAGAACGATTAAAGTCA+TGG 0.511255 6_2:-69648495 Msa0926080:CDS
GAAACAGGTTGGCCTTCAAA+AGG 0.516605 6_2:-69648467 Msa0926080:CDS
CTTGCAGCACTCAAGAAAGC+TGG 0.520860 6_2:-69648839 Msa0926080:CDS
GGAGTAGCAGCTGTCTCCTT+AGG 0.522614 6_2:+69648440 None:intergenic
ACCGGTACACCTGCAAAGCC+GGG 0.530286 6_2:-69648365 Msa0926080:CDS
GTTTCCAGTACTCATTCGCT+TGG 0.531431 6_2:-69648797 Msa0926080:CDS
TCATTTCCTGAAGCCACTGT+TGG 0.532326 6_2:-69648720 Msa0926080:CDS
GCATGCGAGACTAAGTTATC+CGG 0.533664 6_2:+69648032 None:intergenic
GGCTCAAAACAAGGCTGGCT+AGG 0.534132 6_2:+69648053 None:intergenic
CAAGTGACAGGCCAAATAGC+AGG 0.535238 6_2:-69647117 Msa0926080:three_prime_UTR
ACCAGTCCCTCCAAAACTAC+AGG 0.542420 6_2:+69647959 None:intergenic
GCAAGGACATTAGTCATGGC+TGG 0.545995 6_2:+69648857 None:intergenic
GTTCAACGAAAATAGGAAGC+CGG 0.548569 6_2:-69648312 Msa0926080:CDS
CTATTAAAAGCACCGGCGGA+AGG 0.552762 6_2:+69648752 None:intergenic
TAAACGTCTAGCTCTTCTCC+CGG 0.554104 6_2:+69648347 None:intergenic
ATGCTTGCTATTAAAAGCAC+CGG 0.555909 6_2:+69648745 None:intergenic
TATCCGGCTCAAAACAAGGC+TGG 0.556154 6_2:+69648048 None:intergenic
GAAGTTATTGATCCTAACAC+TGG 0.557705 6_2:-69648584 Msa0926080:CDS
GTAGACTTCTTTGCTTGAGG+AGG 0.562432 6_2:+69647420 None:intergenic
TAAAAGCACCGGCGGAAGGA+GGG 0.563613 6_2:+69648756 None:intergenic
GTCTCATTAGTTTCATGTAG+GGG 0.567446 6_2:-69647026 Msa0926080:three_prime_UTR
GCCTTCAAAAGGATCGCCTA+AGG 0.568225 6_2:-69648456 Msa0926080:CDS
TTTGGAATCTGAGAGGAACT+GGG 0.570238 6_2:-69648288 Msa0926080:CDS
TTGTGGTACTTCTGGAAATG+TGG 0.570623 6_2:-69648093 Msa0926080:CDS
TCCAAACTAATGCAGACACT+TGG 0.571386 6_2:-69648985 Msa0926080:CDS
AAGTTATCCGGCTCAAAACA+AGG 0.571467 6_2:+69648044 None:intergenic
ATGTATGTGATCTTGGTGGC+TGG 0.572695 6_2:+69648935 None:intergenic
CAGCTTTCTTGAGTGCTGCA+AGG 0.575770 6_2:+69648840 None:intergenic
AGGATCAATAACTTCGGAGG+AGG 0.583202 6_2:+69648592 None:intergenic
ACTGCATCTACATGAAAACT+GGG 0.585590 6_2:-69647623 Msa0926080:intron
TTAAAAGCACCGGCGGAAGG+AGG 0.587445 6_2:+69648755 None:intergenic
TGAGTAGACTTCTTTGCTTG+AGG 0.590263 6_2:+69647417 None:intergenic
TAGTCCAAAGACACTTTGGT+TGG 0.592684 6_2:+69648629 None:intergenic
TGGTGGCTGGATAGTAAGGA+AGG 0.592958 6_2:+69648948 None:intergenic
ACCTGATAAGGTGTCACAGT+TGG 0.594346 6_2:-69649137 Msa0926080:CDS
CAAGTTTGATCGATGACAAG+AGG 0.595306 6_2:-69647328 Msa0926080:three_prime_UTR
TAGAGAGAACAATGGCAAGA+GGG 0.597917 6_2:-69650568 Msa0926080:exon
GCCACTGTTGGAATTTCTCG+CGG 0.598098 6_2:-69648708 Msa0926080:CDS
AACTGTGACACCTTATCAGG+TGG 0.598360 6_2:+69649139 None:intergenic
TCCGCGAGAAATTCCAACAG+TGG 0.611202 6_2:+69648707 None:intergenic
ATAGAGAGAACAATGGCAAG+AGG 0.613986 6_2:-69650569 Msa0926080:exon
ACTAGTAGCATTTGCATCTG+CGG 0.616757 6_2:+69648196 None:intergenic
TGTGACACCTTATCAGGTGG+AGG 0.617208 6_2:+69649142 None:intergenic
TCTTTGCTTGAGGAGGATGG+AGG 0.619966 6_2:+69647427 None:intergenic
ATGTAGGGGATGTATAACAA+TGG 0.628671 6_2:-69647012 Msa0926080:three_prime_UTR
CACTGCTCTGAAGAGAGTGA+AGG 0.631176 6_2:+69650603 None:intergenic
AATGCGATAAATTGGGCTTG+TGG 0.631670 6_2:-69648110 Msa0926080:CDS
AGTGTTAGGATCAATAACTT+CGG 0.634976 6_2:+69648586 None:intergenic
CTCCATTCTAGACTCTACAC+AGG 0.637784 6_2:-69650687 Msa0926080:five_prime_UTR
TCATGGTAACCGAAACAGGT+TGG 0.641295 6_2:-69648478 Msa0926080:CDS
CATGGCTGGCACAACTAATG+CGG 0.643202 6_2:+69648871 None:intergenic
TCTAGAGATAGAGAGAACAA+TGG 0.645847 6_2:-69650576 Msa0926080:exon
TCCAATCATAAGTTCGACGC+CGG 0.648715 6_2:+69649042 None:intergenic
GTTAGGATCAATAACTTCGG+AGG 0.650725 6_2:+69648589 None:intergenic
CAGATAAAGACATAAAGTAA+CGG 0.651037 6_2:+69650655 None:intergenic
AAGATCACATACATAACTGT+CGG 0.658420 6_2:-69648926 Msa0926080:CDS
TTGGAATCTGAGAGGAACTG+GGG 0.664655 6_2:-69648287 Msa0926080:CDS
CTTGCTATTAAAAGCACCGG+CGG 0.667006 6_2:+69648748 None:intergenic
TGCTGCAAGGACATTAGTCA+TGG 0.668003 6_2:+69648853 None:intergenic
TTTGGCAAGTAATAATACAA+CGG 0.676947 6_2:-69647467 Msa0926080:CDS
AGCCGGGTTTGGAATCTGAG+AGG 0.695818 6_2:-69648295 Msa0926080:CDS
AGTTATGTATGTGATCTTGG+TGG 0.696411 6_2:+69648931 None:intergenic
AAGCAGCATACACCTCAACG+TGG 0.802058 6_2:+69647263 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTCAATGTAATTATAT+GGG + chr6_2:69647662-69647681 None:intergenic 10.0%
!!! AAAAGAAAAAACTATATTTT+TGG - chr6_2:69648026-69648045 Msa0926080:CDS 10.0%
!! AAAATAAATAAGAATTACAA+TGG + chr6_2:69648397-69648416 None:intergenic 10.0%
!! CATAATTCAATGTAATTATA+TGG + chr6_2:69647663-69647682 None:intergenic 15.0%
!!! TTTTAGGTTTAAATATGTTA+TGG - chr6_2:69649818-69649837 Msa0926080:intron 15.0%
!!! AGATTTTCTCAAATTATTAG+CGG - chr6_2:69647683-69647702 Msa0926080:intron 20.0%
!!! TTTTAGTTAATCATGCTAAA+TGG + chr6_2:69648079-69648098 None:intergenic 20.0%
!! AAAAAAACTGAAAAATGGAA+AGG + chr6_2:69649762-69649781 None:intergenic 20.0%
!! ATACGAAAAAAACTGAAAAA+TGG + chr6_2:69649767-69649786 None:intergenic 20.0%
!!! TTTTCGTATACTTATCTTTT+CGG - chr6_2:69649778-69649797 Msa0926080:intron 20.0%
!! GAAAATATTGCATATTTACA+GGG + chr6_2:69649845-69649864 None:intergenic 20.0%
! CAGATAAAGACATAAAGTAA+CGG + chr6_2:69646989-69647008 None:intergenic 25.0%
!! TTTTGAACTAGAAAGAAGAT+AGG - chr6_2:69647412-69647431 Msa0926080:CDS 25.0%
! TATTCAAAATCTATGAAGCA+CGG + chr6_2:69647744-69647763 None:intergenic 25.0%
! AAATGGAACAAAAGTAGTTT+AGG + chr6_2:69648062-69648081 None:intergenic 25.0%
!! AAAACAAAGCTTTGTAACAT+CGG - chr6_2:69648095-69648114 Msa0926080:CDS 25.0%
! TAACCCGAAATATTACTTAA+GGG - chr6_2:69648262-69648281 Msa0926080:CDS 25.0%
!! ATTTTATCAGATTGTTGCAA+TGG - chr6_2:69648433-69648452 Msa0926080:CDS 25.0%
!! TTTTATCAGATTGTTGCAAT+GGG - chr6_2:69648434-69648453 Msa0926080:CDS 25.0%
!!! TGCAATTTATTTTGCGTTAA+CGG - chr6_2:69649116-69649135 Msa0926080:CDS 25.0%
! ACTTACAAAATGCGATAAAT+TGG - chr6_2:69649523-69649542 Msa0926080:intron 25.0%
! CTTACAAAATGCGATAAATT+GGG - chr6_2:69649524-69649543 Msa0926080:intron 25.0%
! AGGCAAATTAATTAAGTGAT+GGG + chr6_2:69649742-69649761 None:intergenic 25.0%
! AAGGCAAATTAATTAAGTGA+TGG + chr6_2:69649743-69649762 None:intergenic 25.0%
! GGAAAATATTGCATATTTAC+AGG + chr6_2:69649846-69649865 None:intergenic 25.0%
! AAAAAAATAGCAAGGACAAA+TGG + chr6_2:69649867-69649886 None:intergenic 25.0%
!!! ATTTGTCCTTGCTATTTTTT+TGG - chr6_2:69649866-69649885 Msa0926080:intron 25.0%
! CAAAGATGAACAAATTTATC+AGG + chr6_2:69649893-69649912 None:intergenic 25.0%
! AAATCATCTACACATATTAC+AGG + chr6_2:69649922-69649941 None:intergenic 25.0%
!! AACCAACAAAGAAATTTTCA+AGG + chr6_2:69650054-69650073 None:intergenic 25.0%
! CTTCAGTCTTAATAATGATA+AGG + chr6_2:69650103-69650122 None:intergenic 25.0%
! TATTATTACTTGCCAAAGTT+TGG + chr6_2:69650171-69650190 None:intergenic 25.0%
! TTTGGCAAGTAATAATACAA+CGG - chr6_2:69650174-69650193 Msa0926080:intron 25.0%
!!! ATTAATTTTTTACCACGTTG+AGG - chr6_2:69650366-69650385 Msa0926080:intron 25.0%
! TACTTTTGTTGCATGTTTCT+TGG - chr6_2:69647196-69647215 Msa0926080:three_prime_UTR 30.0%
TTTCTCATGTCTTCCATAAA+GGG + chr6_2:69647377-69647396 None:intergenic 30.0%
ATTTCTCATGTCTTCCATAA+AGG + chr6_2:69647378-69647397 None:intergenic 30.0%
TTTATGGAAGACATGAGAAA+TGG - chr6_2:69647377-69647396 Msa0926080:CDS 30.0%
TAGACATTAATCTCACTAAG+TGG - chr6_2:69647464-69647483 Msa0926080:CDS 30.0%
!! GTAGTTGATTACTTTTCATG+TGG - chr6_2:69647492-69647511 Msa0926080:intron 30.0%
TTGGTTTATGATGATTAGAC+AGG + chr6_2:69647524-69647543 None:intergenic 30.0%
! TGATTTTGAACTATGAAGCA+CGG - chr6_2:69647581-69647600 Msa0926080:intron 30.0%
TTTATGTTTGTATACACTCG+TGG - chr6_2:69647815-69647834 Msa0926080:intron 30.0%
TAACTCACATTTGAGAGATT+TGG - chr6_2:69647987-69648006 Msa0926080:CDS 30.0%
! AACATCGGTTAAGAATTTGT+TGG - chr6_2:69648110-69648129 Msa0926080:CDS 30.0%
TTTGACTAATGACATTGACA+TGG + chr6_2:69648140-69648159 None:intergenic 30.0%
CTAACCCGAAATATTACTTA+AGG - chr6_2:69648261-69648280 Msa0926080:CDS 30.0%
GATCCCTTAAGTAATATTTC+GGG + chr6_2:69648268-69648287 None:intergenic 30.0%
GGATCCCTTAAGTAATATTT+CGG + chr6_2:69648269-69648288 None:intergenic 30.0%
!!! AGTTTTGTTGGAATTTGCTA+TGG - chr6_2:69648457-69648476 Msa0926080:CDS 30.0%
! CTAAAATCTTTTGCAAACAC+CGG - chr6_2:69648580-69648599 Msa0926080:CDS 30.0%
AATGGAAGCAAATCTAGATT+TGG + chr6_2:69648628-69648647 None:intergenic 30.0%
AAGATCACATACATAACTGT+CGG - chr6_2:69648715-69648734 Msa0926080:CDS 30.0%
! AACTAATGCGGAAATGTTTT+CGG + chr6_2:69648761-69648780 None:intergenic 30.0%
GGATATAGATCGATCATAAA+AGG + chr6_2:69648970-69648989 None:intergenic 30.0%
AGTGTTAGGATCAATAACTT+CGG + chr6_2:69649058-69649077 None:intergenic 30.0%
TTTCAGAACGATTAAAGTCA+TGG - chr6_2:69649146-69649165 Msa0926080:CDS 30.0%
TTATACGTTTGTGCATTATC+AGG + chr6_2:69649225-69649244 None:intergenic 30.0%
GATAACATGTCTTATCAGAT+TGG + chr6_2:69649250-69649269 None:intergenic 30.0%
AGACATGTTATCAATGAAAC+CGG - chr6_2:69649258-69649277 Msa0926080:intron 30.0%
CAACTTCAAATGGAACAAAA+TGG - chr6_2:69649469-69649488 Msa0926080:intron 30.0%
AATAGCTATTACCAGCAAAA+TGG - chr6_2:69649645-69649664 Msa0926080:intron 30.0%
!!! AGCTATTGCATGCTTTTTTT+AGG - chr6_2:69649802-69649821 Msa0926080:intron 30.0%
GTCCTTGAAAATTTCTTTGT+TGG - chr6_2:69650049-69650068 Msa0926080:intron 30.0%
AGGCGTAATACAACAATTTA+CGG + chr6_2:69650083-69650102 None:intergenic 30.0%
! GATCTTTCTCCTTTTTCTTT+TGG - chr6_2:69650273-69650292 Msa0926080:intron 30.0%
! CTCCTTTTTCTTTTGGATAT+CGG - chr6_2:69650280-69650299 Msa0926080:intron 30.0%
!!! TAGTTTTGTATTCAAGTGAC+AGG - chr6_2:69650512-69650531 Msa0926080:intron 30.0%
AAAATGTTCATCCTGCTATT+TGG + chr6_2:69650538-69650557 None:intergenic 30.0%
! ACTTTTGAATGTGGAAGTTT+AGG - chr6_2:69650588-69650607 Msa0926080:five_prime_UTR 30.0%
! AGTCTCATTAGTTTCATGTA+GGG - chr6_2:69650614-69650633 Msa0926080:five_prime_UTR 30.0%
TAGTGAAAAATGACACCAAA+AGG - chr6_2:69650687-69650706 Msa0926080:five_prime_UTR 30.0%
TCTAGAGATAGAGAGAACAA+TGG - chr6_2:69647065-69647084 Msa0926080:three_prime_UTR 35.0%
! TCAGTTTTGCTTATGCTTCT+TGG - chr6_2:69647120-69647139 Msa0926080:three_prime_UTR 35.0%
! AGCTTGTTTGTTACCCTTTA+TGG - chr6_2:69647361-69647380 Msa0926080:CDS 35.0%
TCTCAAATTATTAGCGGTGT+TGG - chr6_2:69647689-69647708 Msa0926080:intron 35.0%
TGGAACAAATGTGGTTGTAA+GGG + chr6_2:69648230-69648249 None:intergenic 35.0%
TTGGAACAAATGTGGTTGTA+AGG + chr6_2:69648231-69648250 None:intergenic 35.0%
TACATCAATTCGAGTGAAAC+TGG + chr6_2:69648296-69648315 None:intergenic 35.0%
CACTCGAATTGATGTAATGT+TGG - chr6_2:69648300-69648319 Msa0926080:CDS 35.0%
CGAATTGATGTAATGTTGGT+TGG - chr6_2:69648304-69648323 Msa0926080:CDS 35.0%
! TTAGTTTGGAACTGAGAGAA+TGG + chr6_2:69648646-69648665 None:intergenic 35.0%
AGTTATGTATGTGATCTTGG+TGG + chr6_2:69648713-69648732 None:intergenic 35.0%
GACAGTTATGTATGTGATCT+TGG + chr6_2:69648716-69648735 None:intergenic 35.0%
! ACTAATGCGGAAATGTTTTC+GGG + chr6_2:69648760-69648779 None:intergenic 35.0%
!! ATGCTTGCTATTAAAAGCAC+CGG + chr6_2:69648899-69648918 None:intergenic 35.0%
GAAGTTATTGATCCTAACAC+TGG - chr6_2:69649057-69649076 Msa0926080:CDS 35.0%
GTATAAAGCAAACCAGTGTT+AGG + chr6_2:69649072-69649091 None:intergenic 35.0%
TTTCGCTGTTCAACGAAAAT+AGG - chr6_2:69649322-69649341 Msa0926080:intron 35.0%
GCTACTAGTTCAACTTCAAA+TGG - chr6_2:69649459-69649478 Msa0926080:intron 35.0%
TGATGGGATATGAACATACT+TGG + chr6_2:69649726-69649745 None:intergenic 35.0%
TCAGGACCAAAAAAATAGCA+AGG + chr6_2:69649875-69649894 None:intergenic 35.0%
AACTGCATCTACATGAAAAC+TGG - chr6_2:69650017-69650036 Msa0926080:intron 35.0%
ACTGCATCTACATGAAAACT+GGG - chr6_2:69650018-69650037 Msa0926080:intron 35.0%
TAAGACTGAAGCATGACATT+TGG - chr6_2:69650112-69650131 Msa0926080:intron 35.0%
AAGACTGAAGCATGACATTT+GGG - chr6_2:69650113-69650132 Msa0926080:intron 35.0%
GTCCGATATCCAAAAGAAAA+AGG + chr6_2:69650285-69650304 None:intergenic 35.0%
! TGTCATCGATCAAACTTGTT+AGG + chr6_2:69650311-69650330 None:intergenic 35.0%
CAAGCTTTCGATTGTTGAAA+AGG - chr6_2:69650336-69650355 Msa0926080:intron 35.0%
! AGCAGGATGAACATTTTATG+TGG - chr6_2:69650541-69650560 Msa0926080:CDS 35.0%
! CAGTCTCATTAGTTTCATGT+AGG - chr6_2:69650613-69650632 Msa0926080:five_prime_UTR 35.0%
! GTCTCATTAGTTTCATGTAG+GGG - chr6_2:69650615-69650634 Msa0926080:five_prime_UTR 35.0%
ATGTAGGGGATGTATAACAA+TGG - chr6_2:69650629-69650648 Msa0926080:five_prime_UTR 35.0%
TTCCTGTGTAGAGTCTAGAA+TGG + chr6_2:69646959-69646978 None:intergenic 40.0%
TGTTCTTGTTCTTGCTTGCT+TGG + chr6_2:69647014-69647033 None:intergenic 40.0%
ATAGAGAGAACAATGGCAAG+AGG - chr6_2:69647072-69647091 Msa0926080:three_prime_UTR 40.0%
TAGAGAGAACAATGGCAAGA+GGG - chr6_2:69647073-69647092 Msa0926080:three_prime_UTR 40.0%
ATAACCGAACACACACAACA+TGG + chr6_2:69647547-69647566 None:intergenic 40.0%
TCCTCAAATTAGAGATGTCC+CGG - chr6_2:69647608-69647627 Msa0926080:intron 40.0%
!! TCGTGGACACTTTAGTTTGA+AGG - chr6_2:69647832-69647851 Msa0926080:intron 40.0%
TGTCACTATCGTACGAATGT+TGG + chr6_2:69647873-69647892 None:intergenic 40.0%
GGAACAAATGTGGTTGTAAG+GGG + chr6_2:69648229-69648248 None:intergenic 40.0%
AATTCGAGTGAAACTGGACT+CGG + chr6_2:69648290-69648309 None:intergenic 40.0%
!! TGTTGCAATGGGAGTTTTGT+TGG - chr6_2:69648445-69648464 Msa0926080:CDS 40.0%
TCCAAACTAATGCAGACACT+TGG - chr6_2:69648656-69648675 Msa0926080:CDS 40.0%
TTCGGGACTTTCTGTAACTT+CGG + chr6_2:69648743-69648762 None:intergenic 40.0%
TAGTCCAAAGACACTTTGGT+TGG + chr6_2:69649015-69649034 None:intergenic 40.0%
TGCATAGTCCAAAGACACTT+TGG + chr6_2:69649019-69649038 None:intergenic 40.0%
GTTAGGATCAATAACTTCGG+AGG + chr6_2:69649055-69649074 None:intergenic 40.0%
AAAGTCATGGTAACCGAAAC+AGG - chr6_2:69649159-69649178 Msa0926080:CDS 40.0%
!! TTTTGAAGGCCAACCTGTTT+CGG + chr6_2:69649175-69649194 None:intergenic 40.0%
GTTCAACGAAAATAGGAAGC+CGG - chr6_2:69649329-69649348 Msa0926080:intron 40.0%
TTCAACGAAAATAGGAAGCC+GGG - chr6_2:69649330-69649349 Msa0926080:intron 40.0%
! TTTGGAATCTGAGAGGAACT+GGG - chr6_2:69649353-69649372 Msa0926080:intron 40.0%
GAACTGGGGAATAGTTTATC+CGG - chr6_2:69649368-69649387 Msa0926080:intron 40.0%
! GTGTACAGCCTAGATTTTAC+TGG - chr6_2:69649402-69649421 Msa0926080:intron 40.0%
ACTAGTAGCATTTGCATCTG+CGG + chr6_2:69649448-69649467 None:intergenic 40.0%
AATGCGATAAATTGGGCTTG+TGG - chr6_2:69649531-69649550 Msa0926080:intron 40.0%
! TTGTGGTACTTCTGGAAATG+TGG - chr6_2:69649548-69649567 Msa0926080:intron 40.0%
AAGTTATCCGGCTCAAAACA+AGG + chr6_2:69649600-69649619 None:intergenic 40.0%
! TCTGATGTTGCCTGTAGTTT+TGG - chr6_2:69649672-69649691 Msa0926080:intron 40.0%
!!! ATGTTGCCTGTAGTTTTGGA+GGG - chr6_2:69649676-69649695 Msa0926080:intron 40.0%
! GACTGGTGTACTTGTTGATA+AGG - chr6_2:69649698-69649717 Msa0926080:intron 40.0%
CATGTAGATGCAGTTGTCAT+AGG + chr6_2:69650012-69650031 None:intergenic 40.0%
!! GGTTAGTTCTGCACAAAAAC+AGG + chr6_2:69650150-69650169 None:intergenic 40.0%
GTGCAGAACTAACCAAACTT+TGG - chr6_2:69650156-69650175 Msa0926080:intron 40.0%
TGAGTAGACTTCTTTGCTTG+AGG + chr6_2:69650227-69650246 None:intergenic 40.0%
CAAGTTTGATCGATGACAAG+AGG - chr6_2:69650313-69650332 Msa0926080:intron 40.0%
! GTTGCTGCAACTTTTGAATG+TGG - chr6_2:69650579-69650598 Msa0926080:exon 40.0%
CTCCATTCTAGACTCTACAC+AGG - chr6_2:69646954-69646973 Msa0926080:three_prime_UTR 45.0%
! GAACAATGGCAAGAGGGTTT+TGG - chr6_2:69647079-69647098 Msa0926080:three_prime_UTR 45.0%
CCAACCATGTTGTGTGTGTT+CGG - chr6_2:69647540-69647559 Msa0926080:intron 45.0%
GTTGTGTGTGTTCGGTTATG+CGG - chr6_2:69647548-69647567 Msa0926080:intron 45.0%
ACCGGGACATCTCTAATTTG+AGG + chr6_2:69647612-69647631 None:intergenic 45.0%
AATTAGAGATGTCCCGGTGT+CGG - chr6_2:69647614-69647633 Msa0926080:intron 45.0%
AATCTATGAAGCACGGACAC+CGG + chr6_2:69647737-69647756 None:intergenic 45.0%
TCAGTCAACGACTAAGTCAG+TGG + chr6_2:69648167-69648186 None:intergenic 45.0%
! AGTCGTTGACTGACATTCGT+TGG - chr6_2:69648174-69648193 Msa0926080:CDS 45.0%
! GTCGTTGACTGACATTCGTT+GGG - chr6_2:69648175-69648194 Msa0926080:CDS 45.0%
! TCGTTGACTGACATTCGTTG+GGG - chr6_2:69648176-69648195 Msa0926080:CDS 45.0%
CCTTGTGGTTGGAACAAATG+TGG + chr6_2:69648239-69648258 None:intergenic 45.0%
CCACATTTGTTCCAACCACA+AGG - chr6_2:69648236-69648255 Msa0926080:CDS 45.0%
!! TATTTCGGGTTAGAGCCTTG+TGG + chr6_2:69648254-69648273 None:intergenic 45.0%
AACTGTGACACCTTATCAGG+TGG + chr6_2:69648505-69648524 None:intergenic 45.0%
! ACCTGATAAGGTGTCACAGT+TGG - chr6_2:69648504-69648523 Msa0926080:CDS 45.0%
ACCAACTGTGACACCTTATC+AGG + chr6_2:69648508-69648527 None:intergenic 45.0%
TCCAATCATAAGTTCGACGC+CGG + chr6_2:69648602-69648621 None:intergenic 45.0%
GCCAAGTGTCTGCATTAGTT+TGG + chr6_2:69648660-69648679 None:intergenic 45.0%
! ATCTTGGTGGCTGGATAGTA+AGG + chr6_2:69648700-69648719 None:intergenic 45.0%
! ATGTATGTGATCTTGGTGGC+TGG + chr6_2:69648709-69648728 None:intergenic 45.0%
TGCTGCAAGGACATTAGTCA+TGG + chr6_2:69648791-69648810 None:intergenic 45.0%
GTTTCCAGTACTCATTCGCT+TGG - chr6_2:69648844-69648863 Msa0926080:CDS 45.0%
!! CTTGCTATTAAAAGCACCGG+CGG + chr6_2:69648896-69648915 None:intergenic 45.0%
TCATTTCCTGAAGCCACTGT+TGG - chr6_2:69648921-69648940 Msa0926080:CDS 45.0%
GAAATTCCAACAGTGGCTTC+AGG + chr6_2:69648930-69648949 None:intergenic 45.0%
GAGTCACGATACGCGTAATA+AGG + chr6_2:69648991-69649010 None:intergenic 45.0%
! CTCTCCAACCAAAGTGTCTT+TGG - chr6_2:69649008-69649027 Msa0926080:CDS 45.0%
AGGATCAATAACTTCGGAGG+AGG + chr6_2:69649052-69649071 None:intergenic 45.0%
TCATGGTAACCGAAACAGGT+TGG - chr6_2:69649163-69649182 Msa0926080:CDS 45.0%
!! GAAACAGGTTGGCCTTCAAA+AGG - chr6_2:69649174-69649193 Msa0926080:CDS 45.0%
! TCCTTAGGCGATCCTTTTGA+AGG + chr6_2:69649189-69649208 None:intergenic 45.0%
!! TAAACGTCTAGCTCTTCTCC+CGG + chr6_2:69649297-69649316 None:intergenic 45.0%
TTCCTCTCAGATTCCAAACC+CGG + chr6_2:69649351-69649370 None:intergenic 45.0%
! GTTTGGAATCTGAGAGGAAC+TGG - chr6_2:69649352-69649371 Msa0926080:intron 45.0%
TTGGAATCTGAGAGGAACTG+GGG - chr6_2:69649354-69649373 Msa0926080:intron 45.0%
! CTGTACACGTTTGTTAGGTC+CGG + chr6_2:69649390-69649409 None:intergenic 45.0%
CTAGGCTGTACACGTTTGTT+AGG + chr6_2:69649395-69649414 None:intergenic 45.0%
! AGCCTAGATTTTACTGGACG+AGG - chr6_2:69649408-69649427 Msa0926080:intron 45.0%
GACCTCGTCCAGTAAAATCT+AGG + chr6_2:69649413-69649432 None:intergenic 45.0%
AATTGGGCTTGTGGTACTTC+TGG - chr6_2:69649540-69649559 Msa0926080:intron 45.0%
GCATGCGAGACTAAGTTATC+CGG + chr6_2:69649612-69649631 None:intergenic 45.0%
!!! CATCAGAAGCACCATTTTGC+TGG + chr6_2:69649659-69649678 None:intergenic 45.0%
!!! GATGTTGCCTGTAGTTTTGG+AGG - chr6_2:69649675-69649694 Msa0926080:intron 45.0%
ACTTCTTTGCTTGAGGAGGA+TGG + chr6_2:69650220-69650239 None:intergenic 45.0%
GTAGACTTCTTTGCTTGAGG+AGG + chr6_2:69650224-69650243 None:intergenic 45.0%
!!! GTTGAGGTGTATGCTGCTTT+TGG - chr6_2:69650382-69650401 Msa0926080:intron 45.0%
!! CACTGCTCTGAAGAGAGTGA+AGG + chr6_2:69647041-69647060 None:intergenic 50.0%
CCGAACACACACAACATGGT+TGG + chr6_2:69647543-69647562 None:intergenic 50.0%
TAAACCCTACGAGACACCGT+CGG + chr6_2:69647969-69647988 None:intergenic 50.0%
TTGTAAGGGGAGACCACACA+AGG + chr6_2:69648216-69648235 None:intergenic 50.0%
! TGACTTGCCTCCACCTGATA+AGG - chr6_2:69648492-69648511 Msa0926080:CDS 50.0%
TGTGACACCTTATCAGGTGG+AGG + chr6_2:69648502-69648521 None:intergenic 50.0%
! ACCGGCGTCGAACTTATGAT+TGG - chr6_2:69648598-69648617 Msa0926080:CDS 50.0%
TGGTGGCTGGATAGTAAGGA+AGG + chr6_2:69648696-69648715 None:intergenic 50.0%
CATGGCTGGCACAACTAATG+CGG + chr6_2:69648773-69648792 None:intergenic 50.0%
GCAAGGACATTAGTCATGGC+TGG + chr6_2:69648787-69648806 None:intergenic 50.0%
! CAGCTTTCTTGAGTGCTGCA+AGG + chr6_2:69648804-69648823 None:intergenic 50.0%
CTTGCAGCACTCAAGAAAGC+TGG - chr6_2:69648802-69648821 Msa0926080:CDS 50.0%
AACGCCAAGCGAATGAGTAC+TGG + chr6_2:69648851-69648870 None:intergenic 50.0%
!! CTATTAAAAGCACCGGCGGA+AGG + chr6_2:69648892-69648911 None:intergenic 50.0%
GCCACTGTTGGAATTTCTCG+CGG - chr6_2:69648933-69648952 Msa0926080:CDS 50.0%
TCCGCGAGAAATTCCAACAG+TGG + chr6_2:69648937-69648956 None:intergenic 50.0%
GCCTTCAAAAGGATCGCCTA+AGG - chr6_2:69649185-69649204 Msa0926080:CDS 50.0%
CGAAAATAGGAAGCCGGGTT+TGG - chr6_2:69649335-69649354 Msa0926080:intron 50.0%
!!! TAGCCAGCCTTGTTTTGAGC+CGG - chr6_2:69649590-69649609 Msa0926080:intron 50.0%
TATCCGGCTCAAAACAAGGC+TGG + chr6_2:69649596-69649615 None:intergenic 50.0%
ACCAGTCCCTCCAAAACTAC+AGG + chr6_2:69649685-69649704 None:intergenic 50.0%
TCTTTGCTTGAGGAGGATGG+AGG + chr6_2:69650217-69650236 None:intergenic 50.0%
AAGCAGCATACACCTCAACG+TGG + chr6_2:69650381-69650400 None:intergenic 50.0%
!!! GTATGCTGCTTTTGGCACGA+AGG - chr6_2:69650390-69650409 Msa0926080:intron 50.0%
CAAGTGACAGGCCAAATAGC+AGG - chr6_2:69650524-69650543 Msa0926080:CDS 50.0%
ACACGACATGTGTCCGACAC+CGG + chr6_2:69647630-69647649 None:intergenic 55.0%
TTGTGTGTGTCCGAGTCCGA+CGG - chr6_2:69647950-69647969 Msa0926080:CDS 55.0%
CTGACATTCGTTGGGGAACC+TGG - chr6_2:69648183-69648202 Msa0926080:CDS 55.0%
ACCTGGTTGACCACCTTGTG+TGG - chr6_2:69648200-69648219 Msa0926080:CDS 55.0%
ACCACACAAGGTGGTCAACC+AGG + chr6_2:69648204-69648223 None:intergenic 55.0%
TAAGGGGAGACCACACAAGG+TGG + chr6_2:69648213-69648232 None:intergenic 55.0%
! TCGGGTTAGAGCCTTGTGGT+TGG + chr6_2:69648250-69648269 None:intergenic 55.0%
!! TAAAAGCACCGGCGGAAGGA+GGG + chr6_2:69648888-69648907 None:intergenic 55.0%
!! TTAAAAGCACCGGCGGAAGG+AGG + chr6_2:69648889-69648908 None:intergenic 55.0%
GGAGTAGCAGCTGTCTCCTT+AGG + chr6_2:69649204-69649223 None:intergenic 55.0%
AACCGGTACACCTGCAAAGC+CGG - chr6_2:69649275-69649294 Msa0926080:intron 55.0%
! AGCCGGGTTTGGAATCTGAG+AGG - chr6_2:69649346-69649365 Msa0926080:intron 55.0%
GCATCTGCGGTCATGTCAAC+AGG + chr6_2:69649435-69649454 None:intergenic 55.0%
! GGCTCAAAACAAGGCTGGCT+AGG + chr6_2:69649591-69649610 None:intergenic 55.0%
!!! GCCTGTAGTTTTGGAGGGAC+TGG - chr6_2:69649681-69649700 Msa0926080:intron 55.0%
CACGACATGTGTCCGACACC+GGG + chr6_2:69647629-69647648 None:intergenic 60.0%
CTACGAGACACCGTCGGACT+CGG + chr6_2:69647963-69647982 None:intergenic 60.0%
GAGTCCGACGGTGTCTCGTA+GGG - chr6_2:69647962-69647981 Msa0926080:CDS 60.0%
ACCGGTACACCTGCAAAGCC+GGG - chr6_2:69649276-69649295 Msa0926080:intron 60.0%
TCCCGGCTTTGCAGGTGTAC+CGG + chr6_2:69649280-69649299 None:intergenic 60.0%
AGCTCTTCTCCCGGCTTTGC+AGG + chr6_2:69649288-69649307 None:intergenic 60.0%
GTCAGTGCCCGTGTCGTGTC+CGG - chr6_2:69647715-69647734 Msa0926080:intron 65.0%
ACGGACACCGGACACGACAC+GGG + chr6_2:69647725-69647744 None:intergenic 65.0%
CACGGACACCGGACACGACA+CGG + chr6_2:69647726-69647745 None:intergenic 65.0%
CGAGTCCGACGGTGTCTCGT+AGG - chr6_2:69647961-69647980 Msa0926080:CDS 65.0%
CGATCGTTCCCTCCTTCCGC+CGG - chr6_2:69648877-69648896 Msa0926080:CDS 65.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 69646949 69650714 69646949 ID=Msa0926080;Name=Msa0926080
chr6_2 mRNA 69646949 69650714 69646949 ID=Msa0926080-mRNA-1;Parent=Msa0926080;Name=Msa0926080-mRNA-1;_AED=0.02;_eAED=0.02;_QI=135|1|1|1|1|1|4|402|507
chr6_2 exon 69646949 69647501 69646949 ID=Msa0926080-mRNA-1:exon:11262;Parent=Msa0926080-mRNA-1
chr6_2 exon 69647624 69647654 69647624 ID=Msa0926080-mRNA-1:exon:11261;Parent=Msa0926080-mRNA-1
chr6_2 exon 69647937 69649220 69647937 ID=Msa0926080-mRNA-1:exon:11260;Parent=Msa0926080-mRNA-1
chr6_2 exon 69650522 69650714 69650522 ID=Msa0926080-mRNA-1:exon:11259;Parent=Msa0926080-mRNA-1
chr6_2 five_prime_UTR 69650580 69650714 69650580 ID=Msa0926080-mRNA-1:five_prime_utr;Parent=Msa0926080-mRNA-1
chr6_2 CDS 69650522 69650579 69650522 ID=Msa0926080-mRNA-1:cds;Parent=Msa0926080-mRNA-1
chr6_2 CDS 69647937 69649220 69647937 ID=Msa0926080-mRNA-1:cds;Parent=Msa0926080-mRNA-1
chr6_2 CDS 69647624 69647654 69647624 ID=Msa0926080-mRNA-1:cds;Parent=Msa0926080-mRNA-1
chr6_2 CDS 69647351 69647501 69647351 ID=Msa0926080-mRNA-1:cds;Parent=Msa0926080-mRNA-1
chr6_2 three_prime_UTR 69646949 69647350 69646949 ID=Msa0926080-mRNA-1:three_prime_utr;Parent=Msa0926080-mRNA-1
Gene Sequence

>Msa0926080

ATGGCAAGAGGGTTTTGGTTCATCTTAGCTGTTGCTTCAGTTTTGCTTATGCTTCTTGATTGTTGCAATGGGAGTTTTGTTGGAATTTGCTATGGAAGAAATGCTGATGACTTGCCTCCACCTGATAAGGTGTCACAGTTGGTTCAAGATCACAAAATCAAATATGTCAGAATCTATGATTCTAACATACAAGTTCTAAAATCTTTTGCAAACACCGGCGTCGAACTTATGATTGGAATTCCAAATCTAGATTTGCTTCCATTCTCTCAGTTCCAAACTAATGCAGACACTTGGCTGAGAAATAGCATCCTTCCTTACTATCCAGCCACCAAGATCACATACATAACTGTCGGTGCCGAAGTTACAGAAAGTCCCGAAAACATTTCCGCATTAGTTGTGCCAGCCATGACTAATGTCCTTGCAGCACTCAAGAAAGCTGGACTTCATAAGAAGATAAAAGTTTCCAGTACTCATTCGCTTGGCGTTTTGTCTCGATCGTTCCCTCCTTCCGCCGGTGCTTTTAATAGCAAGCATGCTCATTTCCTGAAGCCACTGTTGGAATTTCTCGCGGAAAATCAGTCACCTTTTATGATCGATCTATATCCTTATTACGCGTATCGTGACTCTCCAACCAAAGTGTCTTTGGACTATGCATTGTTCGAGTCCTCCTCCGAAGTTATTGATCCTAACACTGGTTTGCTTTATACAAACATGTTTGATGCTCAGATTGATGCAATTTATTTTGCGTTAACGGCGTTGAATTTCAGAACGATTAAAGTCATGGTAACCGAAACAGGTTGGCCTTCAAAAGGATCGCCTAAGGAGACAGCTGCTACTCCTGATAATGCACAAACGTATAATACCAATCTGATAAGACATGTTATCAATGAAACCGGTACACCTGCAAAGCCGGGAGAAGAGCTAGACGTTTACATATTTTCGCTGTTCAACGAAAATAGGAAGCCGGGTTTGGAATCTGAGAGGAACTGGGGAATAGTTTATCCGGACCTAACAAACGTGTACAGCCTAGATTTTACTGGACGAGGTCCTGTTGACATGACCGCAGATGCAAATGCTACTAGTTCAACTTCAAATGGAACAAAATGGTGCATTGCTTCGAGTAACGCAACTCAACTTGACTTACAAAATGCGATAAATTGGGCTTGTGGTACTTCTGGAAATGTGGACTGTACTGCTATTCAGCCTAGCCAGCCTTGTTTTGAGCCGGATAACTTAGTCTCGCATGCTTCATATGCTTTTAATAGCTATTACCAGCAAAATGGTGCTTCTGATGTTGCCTGTAGTTTTGGAGGGACTGGTGTACTTGTTGATAAGGATCCAACCTATGACAACTGCATCTACATGAAAACTGGAACTAACCAAACTTTGGCAAGTAATAATACAACGGCGACATCAATATCTTCACCTCCATCCTCCTCAAGCAAAGAAGTCTACTCATCAATCTCGACTAGTGCTCTTCTTTTGATCTTTCTCCTTTTTCTTTTGGATATCGGACAAGCCTAA
Protein sequence

>Msa0926080

MARGFWFILAVASVLLMLLDCCNGSFVGICYGRNADDLPPPDKVSQLVQDHKIKYVRIYDSNIQVLKSFANTGVELMIGIPNLDLLPFSQFQTNADTWLRNSILPYYPATKITYITVGAEVTESPENISALVVPAMTNVLAALKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFNSKHAHFLKPLLEFLAENQSPFMIDLYPYYAYRDSPTKVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDAIYFALTALNFRTIKVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINETGTPAKPGEELDVYIFSLFNENRKPGLESERNWGIVYPDLTNVYSLDFTGRGPVDMTADANATSSTSNGTKWCIASSNATQLDLQNAINWACGTSGNVDCTAIQPSQPCFEPDNLVSHASYAFNSYYQQNGASDVACSFGGTGVLVDKDPTYDNCIYMKTGTNQTLASNNTTATSISSPPSSSSKEVYSSISTSALLLIFLLFLLDIGQA*