AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0784300


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0784300 MtrunA17_Chr5g0437241 93.103 145 10 0 1 145 111 255 9.84e-95 275
Msa0784300 MtrunA17_Chr1g0181301 43.147 197 89 4 2 176 203 398 1.24e-44 151
Msa0784300 MtrunA17_Chr5g0415931 40.104 192 98 4 1 176 191 381 6.08e-36 128
Msa0784300 MtrunA17_Chr5g0437231 61.468 109 33 2 1 100 181 289 2.06e-34 122
Msa0784300 MtrunA17_Chr1g0208731 39.459 185 81 5 1 176 182 344 5.32e-30 112
Msa0784300 MtrunA17_Chr7g0247731 41.117 197 72 10 3 176 191 366 3.94e-26 102
Msa0784300 MtrunA17_Chr5g0415901 42.000 150 71 3 34 176 188 328 4.54e-26 101
Msa0784300 MtrunA17_Chr7g0246641 37.436 195 88 8 2 176 88 268 1.04e-24 96.7
Msa0784300 MtrunA17_Chr7g0247721 37.563 197 84 9 2 175 227 407 1.54e-24 98.6
Msa0784300 MtrunA17_Chr7g0247701 38.071 197 84 7 2 176 190 370 5.54e-24 96.7
Msa0784300 MtrunA17_Chr3g0143621 29.016 193 106 9 2 174 227 408 9.77e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 27 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAATCTCATTGGAAATT+TGG 0.118149 5_2:-68833071 None:intergenic
TAGCATTTGTTTAAACTTTC+CGG 0.160572 5_2:-68832790 None:intergenic
AGCAGCAGCCACCACCTTTC+TGG 0.214961 5_2:+68833042 Msa0784300:CDS
TTTATGGATGTTATGTTCTA+TGG 0.218295 5_2:+68832552 None:intergenic
GATAACTCCAAATCCATTTC+AGG 0.313372 5_2:+68832638 Msa0784300:CDS
ACTTGTGGTCCATTTCATAT+AGG 0.341950 5_2:+68832957 Msa0784300:CDS
GCTATGTCCTGAAATGGATT+TGG 0.374010 5_2:-68832645 None:intergenic
TGAAAAGCTATGTCCTGAAA+TGG 0.396599 5_2:-68832651 None:intergenic
GCAGCAGCCACCACCTTTCT+GGG 0.398247 5_2:+68833043 Msa0784300:CDS
CTTGTGGTCCATTTCATATA+GGG 0.417934 5_2:+68832958 Msa0784300:CDS
TTATATATCCCTATATGAAA+TGG 0.424795 5_2:-68832966 None:intergenic
CGAATTCAAACTTGTACACT+TGG 0.434747 5_2:-68832758 None:intergenic
TTATCTTGTGAAATCATTAG+AGG 0.436221 5_2:+68832689 Msa0784300:CDS
ATGCTAAAGCTTGAGAGTCT+TGG 0.465110 5_2:+68832807 Msa0784300:CDS
GTATCAGCTTCATACTTCTC+CGG 0.473456 5_2:+68832870 Msa0784300:CDS
GGGATATATAATGTAAGAGA+TGG 0.535003 5_2:+68832978 Msa0784300:CDS
ATGGATGTTATGTTCTATGG+AGG 0.548376 5_2:+68832555 Msa0784300:CDS
AAATTTGGTAAAACCCAGAA+AGG 0.548520 5_2:-68833056 None:intergenic
TTTGAATTCGATGCTCAGAC+CGG 0.560810 5_2:+68832771 Msa0784300:CDS
TATCTTGTGAAATCATTAGA+GGG 0.568311 5_2:+68832690 Msa0784300:CDS
GTGAAGATTATGATGATCAG+AGG 0.588224 5_2:-68833003 None:intergenic
GGTAAAACCCAGAAAGGTGG+TGG 0.590899 5_2:-68833050 None:intergenic
AAGTTAGATTGTGTAACTTG+TGG 0.613074 5_2:+68832942 Msa0784300:CDS
AATCATTAGAGGGAGACTTG+TGG 0.640892 5_2:+68832700 Msa0784300:CDS
TTTGGTAAAACCCAGAAAGG+TGG 0.643098 5_2:-68833053 None:intergenic
TATGTTGCGAGTAAATATCA+CGG 0.663367 5_2:+68832585 Msa0784300:CDS
ATTAGAGGGAGACTTGTGGA+TGG 0.690554 5_2:+68832704 Msa0784300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATATCCCTATATGAAA+TGG - chr5_2:68832969-68832988 None:intergenic 20.0%
!! GGTATAGTATCTTTTAATCT+TGG + chr5_2:68832606-68832625 Msa0784300:CDS 25.0%
! TTATCTTGTGAAATCATTAG+AGG + chr5_2:68832689-68832708 Msa0784300:CDS 25.0%
! TATCTTGTGAAATCATTAGA+GGG + chr5_2:68832690-68832709 Msa0784300:CDS 25.0%
! TAGCATTTGTTTAAACTTTC+CGG - chr5_2:68832793-68832812 None:intergenic 25.0%
TATGTTGCGAGTAAATATCA+CGG + chr5_2:68832585-68832604 Msa0784300:CDS 30.0%
! CTTTTAATCTTGGTCATAAG+AGG + chr5_2:68832616-68832635 Msa0784300:CDS 30.0%
!! TGGAGACAATGTTTTATTCT+TGG + chr5_2:68832827-68832846 Msa0784300:CDS 30.0%
!! GGAGACAATGTTTTATTCTT+GGG + chr5_2:68832828-68832847 Msa0784300:CDS 30.0%
!!! TGAATCTTTTTTCAGACAAC+CGG - chr5_2:68832892-68832911 None:intergenic 30.0%
AAGTTAGATTGTGTAACTTG+TGG + chr5_2:68832942-68832961 Msa0784300:CDS 30.0%
GGGATATATAATGTAAGAGA+TGG + chr5_2:68832978-68832997 Msa0784300:CDS 30.0%
AAATTTGGTAAAACCCAGAA+AGG - chr5_2:68833059-68833078 None:intergenic 30.0%
ATGGATGTTATGTTCTATGG+AGG + chr5_2:68832555-68832574 Msa0784300:CDS 35.0%
GATAACTCCAAATCCATTTC+AGG + chr5_2:68832638-68832657 Msa0784300:CDS 35.0%
TGAAAAGCTATGTCCTGAAA+TGG - chr5_2:68832654-68832673 None:intergenic 35.0%
! TTTCAGGACATAGCTTTTCA+TGG + chr5_2:68832654-68832673 Msa0784300:CDS 35.0%
! GCTTTTCATGGAAGGTTTAA+AGG + chr5_2:68832666-68832685 Msa0784300:CDS 35.0%
CGAATTCAAACTTGTACACT+TGG - chr5_2:68832761-68832780 None:intergenic 35.0%
ACTTGTGGTCCATTTCATAT+AGG + chr5_2:68832957-68832976 Msa0784300:CDS 35.0%
CTTGTGGTCCATTTCATATA+GGG + chr5_2:68832958-68832977 Msa0784300:CDS 35.0%
GTGAAGATTATGATGATCAG+AGG - chr5_2:68833006-68833025 None:intergenic 35.0%
! GCTATGTCCTGAAATGGATT+TGG - chr5_2:68832648-68832667 None:intergenic 40.0%
! AGGACATAGCTTTTCATGGA+AGG + chr5_2:68832658-68832677 Msa0784300:CDS 40.0%
AATCATTAGAGGGAGACTTG+TGG + chr5_2:68832700-68832719 Msa0784300:CDS 40.0%
TTTGAATTCGATGCTCAGAC+CGG + chr5_2:68832771-68832790 Msa0784300:CDS 40.0%
ATGCTAAAGCTTGAGAGTCT+TGG + chr5_2:68832807-68832826 Msa0784300:CDS 40.0%
GTATCAGCTTCATACTTCTC+CGG + chr5_2:68832870-68832889 Msa0784300:CDS 40.0%
TTTGGTAAAACCCAGAAAGG+TGG - chr5_2:68833056-68833075 None:intergenic 40.0%
ATTAGAGGGAGACTTGTGGA+TGG + chr5_2:68832704-68832723 Msa0784300:CDS 45.0%
GGTAAAACCCAGAAAGGTGG+TGG - chr5_2:68833053-68833072 None:intergenic 50.0%
AGCAGCAGCCACCACCTTTC+TGG + chr5_2:68833042-68833061 Msa0784300:CDS 60.0%
GCAGCAGCCACCACCTTTCT+GGG + chr5_2:68833043-68833062 Msa0784300:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 68832555 68833085 68832555 ID=Msa0784300;Name=Msa0784300
chr5_2 mRNA 68832555 68833085 68832555 ID=Msa0784300-mRNA-1;Parent=Msa0784300;Name=Msa0784300-mRNA-1;_AED=0.89;_eAED=0.89;_QI=0|-1|0|1|-1|1|1|0|176
chr5_2 exon 68832555 68833085 68832555 ID=Msa0784300-mRNA-1:exon:20666;Parent=Msa0784300-mRNA-1
chr5_2 CDS 68832555 68833085 68832555 ID=Msa0784300-mRNA-1:cds;Parent=Msa0784300-mRNA-1
Gene Sequence

>Msa0784300

ATGGATGTTATGTTCTATGGAGGTTTAGTTTATGTTGCGAGTAAATATCACGGTATAGTATCTTTTAATCTTGGTCATAAGAGGATAACTCCAAATCCATTTCAGGACATAGCTTTTCATGGAAGGTTTAAAGGTTATCTTGTGAAATCATTAGAGGGAGACTTGTGGATGGTGAAAAGATTTTTTACTTTTAAGATTGATTTCCAAGTGTACAAGTTTGAATTCGATGCTCAGACCGGAAAGTTTAAACAAATGCTAAAGCTTGAGAGTCTTGGAGACAATGTTTTATTCTTGGGAGACACTGATTCAATTTCTGTATCAGCTTCATACTTCTCCGGTTGTCTGAAAAAAGATTCAATTTATTACACAGAAAGTATTAATGCAAACAAGTTAGATTGTGTAACTTGTGGTCCATTTCATATAGGGATATATAATGTAAGAGATGGAACCTCTGATCATCATAATCTTCACAATCATTTCTTCAACAAGCAGCAGCCACCACCTTTCTGGGTTTTACCAAATTTCCAATGA

Protein sequence

>Msa0784300

MDVMFYGGLVYVASKYHGIVSFNLGHKRITPNPFQDIAFHGRFKGYLVKSLEGDLWMVKRFFTFKIDFQVYKFEFDAQTGKFKQMLKLESLGDNVLFLGDTDSISVSASYFSGCLKKDSIYYTESINANKLDCVTCGPFHIGIYNVRDGTSDHHNLHNHFFNKQQPPPFWVLPNFQ*