AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1331080


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1331080 MtrunA17_Chr4g0060111 95.181 166 8 0 19 184 1 166 8.11e-118 335
Msa1331080 MtrunA17_Chr4g0060151 95.181 166 8 0 19 184 1 166 8.11e-118 335
Msa1331080 MtrunA17_Chr4g0060091 94.012 167 10 0 18 184 5 171 5.01e-117 333
Msa1331080 MtrunA17_Chr4g0060101 92.771 166 12 0 19 184 1 166 5.06e-115 328
Msa1331080 MtrunA17_Chr4g0060171 88.571 175 11 1 19 184 1 175 1.04e-112 322
Msa1331080 MtrunA17_Chr8g0362631 77.059 170 39 0 19 188 1 170 1.78e-99 289
Msa1331080 MtrunA17_Chr6g0452801 78.313 166 36 0 19 184 1 166 3.41e-95 278
Msa1331080 MtrunA17_Chr6g0476481 55.294 170 74 1 19 186 1 170 3.56e-59 186
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1331080 AT5G62740.1 83.735 166 27 0 19 184 1 166 6.60e-103 298
Msa1331080 AT1G69840.1 77.381 168 38 0 19 186 1 168 3.05e-97 283
Msa1331080 AT1G69840.2 77.381 168 38 0 19 186 1 168 3.05e-97 283
Msa1331080 AT1G69840.5 77.381 168 38 0 19 186 1 168 3.05e-97 283
Msa1331080 AT1G69840.7 77.381 168 38 0 19 186 1 168 3.05e-97 283
Msa1331080 AT1G69840.4 77.381 168 38 0 19 186 1 168 3.05e-97 283
Msa1331080 AT1G69840.6 77.381 168 38 0 19 186 1 168 3.05e-97 283
Msa1331080 AT1G69840.3 77.381 168 38 0 19 186 1 168 3.05e-97 283
Msa1331080 AT3G01290.1 76.647 167 39 0 19 185 1 167 5.65e-92 270
Msa1331080 AT5G51570.1 54.118 170 76 1 19 186 1 170 4.52e-60 189

Find 33 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATTGCAGTGAAAAGAAAA+TGG 0.251403 8_4:+50472942 Msa1331080:CDS
GCATCATCCAAGTTGAGTTT+TGG 0.266671 8_4:-50473691 None:intergenic
CATTGCATGCCATGGTTTCT+TGG 0.290257 8_4:+50473057 Msa1331080:CDS
ATGTTCTCATTGCAGTGATT+AGG 0.311568 8_4:+50473659 Msa1331080:intron
TGTTCTCATTGCAGTGATTA+GGG 0.312025 8_4:+50473660 Msa1331080:intron
CAACAGGCTATGTCAGCTTA+TGG 0.321761 8_4:+50473834 Msa1331080:intron
CACTCTTCGGCTACAACAAT+TGG 0.343058 8_4:+50473101 Msa1331080:CDS
TTTCTTGGAAAACGAATTGC+TGG 0.394254 8_4:+50473072 Msa1331080:CDS
GTGGCTATGAGAGAAGGTTT+TGG 0.398028 8_4:+50473003 Msa1331080:CDS
GCCGCCTTCACATGCTGATC+TGG 0.418175 8_4:-50473894 None:intergenic
CATTGCAGTGAAAAGAAAAT+GGG 0.429423 8_4:+50472943 Msa1331080:CDS
ATTTGAAAAGGTGCTTCAGC+CGG 0.463345 8_4:+50473029 Msa1331080:CDS
TTGCTGGTCATCTCACTCTT+CGG 0.485262 8_4:+50473088 Msa1331080:CDS
TTCTATTCAATACCGTGCCT+TGG 0.491841 8_4:+50473472 Msa1331080:CDS
CGGGATGCCATTGCATGCCA+TGG 0.503619 8_4:+50473049 Msa1331080:CDS
TTTGAAAAGGTGCTTCAGCC+GGG 0.514758 8_4:+50473030 Msa1331080:CDS
CATGGCATGCAATGGCATCC+CGG 0.521469 8_4:-50473048 None:intergenic
TAACAAACCAACCTTTGTCT+TGG 0.535367 8_4:-50473139 None:intergenic
AAGCGTTCCAAAACTCAACT+TGG 0.571578 8_4:+50473684 Msa1331080:CDS
GCTATGAATGAAATCAATGC+TGG 0.578813 8_4:+50473915 Msa1331080:CDS
CAAGAAACCATGGCATGCAA+TGG 0.579517 8_4:-50473056 None:intergenic
AAATGAAATCGCAAAAGCTG+TGG 0.586140 8_4:+50473726 Msa1331080:CDS
ATACCGTGCCTTGGCAGACA+AGG 0.587225 8_4:+50473481 Msa1331080:CDS
TCTCAAGTGGCTATGAGAGA+AGG 0.594736 8_4:+50472997 Msa1331080:CDS
GCCAGATCAGCATGTGAAGG+CGG 0.594901 8_4:+50473893 Msa1331080:CDS
ATCATTAGCCTTGTCTGCCA+AGG 0.596483 8_4:-50473489 None:intergenic
TGTGGAAGAAGAACTTGAGA+AGG 0.603618 8_4:+50473744 Msa1331080:CDS
GCAAGTTGATCAATCTCAAG+TGG 0.619072 8_4:+50472984 Msa1331080:CDS
TAGCCTTGTCTGCCAAGGCA+CGG 0.623988 8_4:-50473484 None:intergenic
TTTACAAACTTAGCAACACA+AGG 0.624349 8_4:+50473516 Msa1331080:CDS
AGAGCCAGATCAGCATGTGA+AGG 0.648209 8_4:+50473890 Msa1331080:CDS
CAAATGTGAGACCAAGACAA+AGG 0.650717 8_4:+50473128 Msa1331080:CDS
ATTGCAGTGAAAAGAAAATG+GGG 0.673791 8_4:+50472944 Msa1331080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGAAAGTATTTATGTTAT+TGG - chr8_4:50473219-50473238 None:intergenic 15.0%
!!! TTTAGCTAGAATATTTTTTT+AGG + chr8_4:50473238-50473257 Msa1331080:intron 15.0%
!! AATATGCAAATTATCTAATA+AGG + chr8_4:50473628-50473647 Msa1331080:intron 15.0%
!!! AAAGAAAGTATTTATGTTAT+TGG - chr8_4:50473219-50473238 None:intergenic 15.0%
!!! TTTAGCTAGAATATTTTTTT+AGG + chr8_4:50473238-50473257 Msa1331080:intron 15.0%
!! AATATGCAAATTATCTAATA+AGG + chr8_4:50473628-50473647 Msa1331080:intron 15.0%
!!! GTCTGGTTTTAATATTTATT+TGG + chr8_4:50473417-50473436 Msa1331080:intron 20.0%
!! TTTAATATTTATTTGGTTGC+AGG + chr8_4:50473424-50473443 Msa1331080:intron 20.0%
!!! ACAAATAGTATTTACTGATT+AGG + chr8_4:50473778-50473797 Msa1331080:intron 20.0%
!!! TAATTTTGAATCATATCAAC+AGG + chr8_4:50473818-50473837 Msa1331080:intron 20.0%
!!! GTCTGGTTTTAATATTTATT+TGG + chr8_4:50473417-50473436 Msa1331080:intron 20.0%
!! TTTAATATTTATTTGGTTGC+AGG + chr8_4:50473424-50473443 Msa1331080:intron 20.0%
!!! ACAAATAGTATTTACTGATT+AGG + chr8_4:50473778-50473797 Msa1331080:intron 20.0%
!!! TAATTTTGAATCATATCAAC+AGG + chr8_4:50473818-50473837 Msa1331080:intron 20.0%
!!! AGGTTTTGGAAAATTTGAAA+AGG + chr8_4:50473017-50473036 Msa1331080:CDS 25.0%
! ATTTATGTTATTGGTAAGCA+TGG - chr8_4:50473210-50473229 None:intergenic 25.0%
!!! TATTTTTTTAGGTGTCATCT+TGG + chr8_4:50473249-50473268 Msa1331080:intron 25.0%
! CTTATATGTTTGCTTAAGTA+TGG + chr8_4:50473290-50473309 Msa1331080:intron 25.0%
!!! TACATATGAAGTATTGAAAG+TGG - chr8_4:50473341-50473360 None:intergenic 25.0%
! TCCATTAAATTAATGTTGTC+TGG + chr8_4:50473400-50473419 Msa1331080:intron 25.0%
! ACCAGACAACATTAATTTAA+TGG - chr8_4:50473404-50473423 None:intergenic 25.0%
!!! ATTCAAGCTTATGTTTTTGA+TGG + chr8_4:50473545-50473564 Msa1331080:CDS 25.0%
!!! AGGTTTTGGAAAATTTGAAA+AGG + chr8_4:50473017-50473036 Msa1331080:CDS 25.0%
! ATTTATGTTATTGGTAAGCA+TGG - chr8_4:50473210-50473229 None:intergenic 25.0%
!!! TATTTTTTTAGGTGTCATCT+TGG + chr8_4:50473249-50473268 Msa1331080:intron 25.0%
! CTTATATGTTTGCTTAAGTA+TGG + chr8_4:50473290-50473309 Msa1331080:intron 25.0%
!!! TACATATGAAGTATTGAAAG+TGG - chr8_4:50473341-50473360 None:intergenic 25.0%
! TCCATTAAATTAATGTTGTC+TGG + chr8_4:50473400-50473419 Msa1331080:intron 25.0%
! ACCAGACAACATTAATTTAA+TGG - chr8_4:50473404-50473423 None:intergenic 25.0%
!!! ATTCAAGCTTATGTTTTTGA+TGG + chr8_4:50473545-50473564 Msa1331080:CDS 25.0%
CATTGCAGTGAAAAGAAAAT+GGG + chr8_4:50472943-50472962 Msa1331080:CDS 30.0%
ATTGCAGTGAAAAGAAAATG+GGG + chr8_4:50472944-50472963 Msa1331080:CDS 30.0%
TTTACAAACTTAGCAACACA+AGG + chr8_4:50473516-50473535 Msa1331080:CDS 30.0%
CATTGCAGTGAAAAGAAAAT+GGG + chr8_4:50472943-50472962 Msa1331080:CDS 30.0%
ATTGCAGTGAAAAGAAAATG+GGG + chr8_4:50472944-50472963 Msa1331080:CDS 30.0%
TTTACAAACTTAGCAACACA+AGG + chr8_4:50473516-50473535 Msa1331080:CDS 30.0%
GCATTGCAGTGAAAAGAAAA+TGG + chr8_4:50472942-50472961 Msa1331080:CDS 35.0%
! ATTCGTTTTCCAAGAAACCA+TGG - chr8_4:50473069-50473088 None:intergenic 35.0%
TTTCTTGGAAAACGAATTGC+TGG + chr8_4:50473072-50473091 Msa1331080:CDS 35.0%
TAACAAACCAACCTTTGTCT+TGG - chr8_4:50473142-50473161 None:intergenic 35.0%
! GTTTGTTACTGTCTCAAACT+TGG + chr8_4:50473154-50473173 Msa1331080:intron 35.0%
CATGGAATGACATGAAGATT+TGG - chr8_4:50473192-50473211 None:intergenic 35.0%
ATCTCTTTCACTATTGTGTC+AGG + chr8_4:50473315-50473334 Msa1331080:intron 35.0%
GTACATAAACATGCAACAAG+TGG + chr8_4:50473599-50473618 Msa1331080:intron 35.0%
ATGTTCTCATTGCAGTGATT+AGG + chr8_4:50473659-50473678 Msa1331080:intron 35.0%
TGTTCTCATTGCAGTGATTA+GGG + chr8_4:50473660-50473679 Msa1331080:intron 35.0%
AAATGAAATCGCAAAAGCTG+TGG + chr8_4:50473726-50473745 Msa1331080:CDS 35.0%
GCTATGAATGAAATCAATGC+TGG + chr8_4:50473915-50473934 Msa1331080:CDS 35.0%
GCATTGCAGTGAAAAGAAAA+TGG + chr8_4:50472942-50472961 Msa1331080:CDS 35.0%
! ATTCGTTTTCCAAGAAACCA+TGG - chr8_4:50473069-50473088 None:intergenic 35.0%
TTTCTTGGAAAACGAATTGC+TGG + chr8_4:50473072-50473091 Msa1331080:CDS 35.0%
TAACAAACCAACCTTTGTCT+TGG - chr8_4:50473142-50473161 None:intergenic 35.0%
! GTTTGTTACTGTCTCAAACT+TGG + chr8_4:50473154-50473173 Msa1331080:intron 35.0%
CATGGAATGACATGAAGATT+TGG - chr8_4:50473192-50473211 None:intergenic 35.0%
ATCTCTTTCACTATTGTGTC+AGG + chr8_4:50473315-50473334 Msa1331080:intron 35.0%
GTACATAAACATGCAACAAG+TGG + chr8_4:50473599-50473618 Msa1331080:intron 35.0%
ATGTTCTCATTGCAGTGATT+AGG + chr8_4:50473659-50473678 Msa1331080:intron 35.0%
TGTTCTCATTGCAGTGATTA+GGG + chr8_4:50473660-50473679 Msa1331080:intron 35.0%
AAATGAAATCGCAAAAGCTG+TGG + chr8_4:50473726-50473745 Msa1331080:CDS 35.0%
GCTATGAATGAAATCAATGC+TGG + chr8_4:50473915-50473934 Msa1331080:CDS 35.0%
!! GCAAGTTGATCAATCTCAAG+TGG + chr8_4:50472984-50473003 Msa1331080:CDS 40.0%
!! ATTTGAAAAGGTGCTTCAGC+CGG + chr8_4:50473029-50473048 Msa1331080:CDS 40.0%
CAAATGTGAGACCAAGACAA+AGG + chr8_4:50473128-50473147 Msa1331080:CDS 40.0%
TTCTATTCAATACCGTGCCT+TGG + chr8_4:50473472-50473491 Msa1331080:CDS 40.0%
AAGCGTTCCAAAACTCAACT+TGG + chr8_4:50473684-50473703 Msa1331080:CDS 40.0%
!! GCATCATCCAAGTTGAGTTT+TGG - chr8_4:50473694-50473713 None:intergenic 40.0%
TGTGGAAGAAGAACTTGAGA+AGG + chr8_4:50473744-50473763 Msa1331080:CDS 40.0%
!! GCAAGTTGATCAATCTCAAG+TGG + chr8_4:50472984-50473003 Msa1331080:CDS 40.0%
!! ATTTGAAAAGGTGCTTCAGC+CGG + chr8_4:50473029-50473048 Msa1331080:CDS 40.0%
CAAATGTGAGACCAAGACAA+AGG + chr8_4:50473128-50473147 Msa1331080:CDS 40.0%
TTCTATTCAATACCGTGCCT+TGG + chr8_4:50473472-50473491 Msa1331080:CDS 40.0%
AAGCGTTCCAAAACTCAACT+TGG + chr8_4:50473684-50473703 Msa1331080:CDS 40.0%
!! GCATCATCCAAGTTGAGTTT+TGG - chr8_4:50473694-50473713 None:intergenic 40.0%
TGTGGAAGAAGAACTTGAGA+AGG + chr8_4:50473744-50473763 Msa1331080:CDS 40.0%
TCTCAAGTGGCTATGAGAGA+AGG + chr8_4:50472997-50473016 Msa1331080:CDS 45.0%
! GTGGCTATGAGAGAAGGTTT+TGG + chr8_4:50473003-50473022 Msa1331080:CDS 45.0%
!! TTTGAAAAGGTGCTTCAGCC+GGG + chr8_4:50473030-50473049 Msa1331080:CDS 45.0%
CAAGAAACCATGGCATGCAA+TGG - chr8_4:50473059-50473078 None:intergenic 45.0%
CATTGCATGCCATGGTTTCT+TGG + chr8_4:50473057-50473076 Msa1331080:CDS 45.0%
TTGCTGGTCATCTCACTCTT+CGG + chr8_4:50473088-50473107 Msa1331080:CDS 45.0%
CACTCTTCGGCTACAACAAT+TGG + chr8_4:50473101-50473120 Msa1331080:CDS 45.0%
TGTGAGACCAAGACAAAGGT+TGG + chr8_4:50473132-50473151 Msa1331080:intron 45.0%
!! ATCATTAGCCTTGTCTGCCA+AGG - chr8_4:50473492-50473511 None:intergenic 45.0%
CAACAGGCTATGTCAGCTTA+TGG + chr8_4:50473834-50473853 Msa1331080:intron 45.0%
TCTCAAGTGGCTATGAGAGA+AGG + chr8_4:50472997-50473016 Msa1331080:CDS 45.0%
! GTGGCTATGAGAGAAGGTTT+TGG + chr8_4:50473003-50473022 Msa1331080:CDS 45.0%
!! TTTGAAAAGGTGCTTCAGCC+GGG + chr8_4:50473030-50473049 Msa1331080:CDS 45.0%
CAAGAAACCATGGCATGCAA+TGG - chr8_4:50473059-50473078 None:intergenic 45.0%
CATTGCATGCCATGGTTTCT+TGG + chr8_4:50473057-50473076 Msa1331080:CDS 45.0%
TTGCTGGTCATCTCACTCTT+CGG + chr8_4:50473088-50473107 Msa1331080:CDS 45.0%
CACTCTTCGGCTACAACAAT+TGG + chr8_4:50473101-50473120 Msa1331080:CDS 45.0%
TGTGAGACCAAGACAAAGGT+TGG + chr8_4:50473132-50473151 Msa1331080:intron 45.0%
!! ATCATTAGCCTTGTCTGCCA+AGG - chr8_4:50473492-50473511 None:intergenic 45.0%
CAACAGGCTATGTCAGCTTA+TGG + chr8_4:50473834-50473853 Msa1331080:intron 45.0%
AGAGCCAGATCAGCATGTGA+AGG + chr8_4:50473890-50473909 Msa1331080:CDS 50.0%
AGAGCCAGATCAGCATGTGA+AGG + chr8_4:50473890-50473909 Msa1331080:CDS 50.0%
CATGGCATGCAATGGCATCC+CGG - chr8_4:50473051-50473070 None:intergenic 55.0%
ATACCGTGCCTTGGCAGACA+AGG + chr8_4:50473481-50473500 Msa1331080:CDS 55.0%
!! TAGCCTTGTCTGCCAAGGCA+CGG - chr8_4:50473487-50473506 None:intergenic 55.0%
GCCAGATCAGCATGTGAAGG+CGG + chr8_4:50473893-50473912 Msa1331080:CDS 55.0%
CATGGCATGCAATGGCATCC+CGG - chr8_4:50473051-50473070 None:intergenic 55.0%
ATACCGTGCCTTGGCAGACA+AGG + chr8_4:50473481-50473500 Msa1331080:CDS 55.0%
!! TAGCCTTGTCTGCCAAGGCA+CGG - chr8_4:50473487-50473506 None:intergenic 55.0%
GCCAGATCAGCATGTGAAGG+CGG + chr8_4:50473893-50473912 Msa1331080:CDS 55.0%
CGGGATGCCATTGCATGCCA+TGG + chr8_4:50473049-50473068 Msa1331080:CDS 60.0%
GCCGCCTTCACATGCTGATC+TGG - chr8_4:50473897-50473916 None:intergenic 60.0%
CGGGATGCCATTGCATGCCA+TGG + chr8_4:50473049-50473068 Msa1331080:CDS 60.0%
GCCGCCTTCACATGCTGATC+TGG - chr8_4:50473897-50473916 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 50472907 50473950 50472907 ID=Msa1331080;Name=Msa1331080
chr8_4 mRNA 50472907 50473950 50472907 ID=Msa1331080-mRNA-1;Parent=Msa1331080;Name=Msa1331080-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|1|0.5|1|1|1|4|0|188
chr8_4 exon 50472907 50473149 50472907 ID=Msa1331080-mRNA-1:exon:14728;Parent=Msa1331080-mRNA-1
chr8_4 exon 50473446 50473566 50473446 ID=Msa1331080-mRNA-1:exon:14729;Parent=Msa1331080-mRNA-1
chr8_4 exon 50473674 50473765 50473674 ID=Msa1331080-mRNA-1:exon:14730;Parent=Msa1331080-mRNA-1
chr8_4 exon 50473840 50473950 50473840 ID=Msa1331080-mRNA-1:exon:14731;Parent=Msa1331080-mRNA-1
chr8_4 CDS 50472907 50473149 50472907 ID=Msa1331080-mRNA-1:cds;Parent=Msa1331080-mRNA-1
chr8_4 CDS 50473446 50473566 50473446 ID=Msa1331080-mRNA-1:cds;Parent=Msa1331080-mRNA-1
chr8_4 CDS 50473674 50473765 50473674 ID=Msa1331080-mRNA-1:cds;Parent=Msa1331080-mRNA-1
chr8_4 CDS 50473840 50473950 50473840 ID=Msa1331080-mRNA-1:cds;Parent=Msa1331080-mRNA-1
Gene Sequence

>Msa1331080

ATGATAAAATTATTCTGTGTTGATGATGTTTTGAAGCATTGCAGTGAAAAGAAAATGGGGAATCTTATGTGTTGCGTGCAAGTTGATCAATCTCAAGTGGCTATGAGAGAAGGTTTTGGAAAATTTGAAAAGGTGCTTCAGCCGGGATGCCATTGCATGCCATGGTTTCTTGGAAAACGAATTGCTGGTCATCTCACTCTTCGGCTACAACAATTGGATATCAAATGTGAGACCAAGACAAAGGATAATGTGTTTGTCAATGTTGTTGCTTCTATTCAATACCGTGCCTTGGCAGACAAGGCTAATGATGCATTTTACAAACTTAGCAACACAAGGAAACAAATTCAAGCTTATGTTTTTGATGTGATTAGGGCAAGCGTTCCAAAACTCAACTTGGATGATGCTTTTGAGCAGAAAAATGAAATCGCAAAAGCTGTGGAAGAAGAACTTGAGAAGGCTATGTCAGCTTATGGATATGAAATTGTTCAAACACTGATTACTGATATAGAGCCAGATCAGCATGTGAAGGCGGCTATGAATGAAATCAATGCTGGTAAGTTTTTTTAA

Protein sequence

>Msa1331080

MIKLFCVDDVLKHCSEKKMGNLMCCVQVDQSQVAMREGFGKFEKVLQPGCHCMPWFLGKRIAGHLTLRLQQLDIKCETKTKDNVFVNVVASIQYRALADKANDAFYKLSNTRKQIQAYVFDVIRASVPKLNLDDAFEQKNEIAKAVEEELEKAMSAYGYEIVQTLITDIEPDQHVKAAMNEINAGKFF*