Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60395.t1 | XP_003608632.1 | 99.1 | 229 | 2 | 0 | 1 | 229 | 1 | 229 | 4.40E-133 | 483.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60395.t1 | G7JGH6 | 99.1 | 229 | 2 | 0 | 1 | 229 | 1 | 229 | 3.2e-133 | 483.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene60395 | 0.888491 | 5.63E-73 | -1.69E-46 |
| MS.gene049171 | MS.gene60395 | 0.81835 | 2.05E-52 | -1.69E-46 |
| MS.gene049217 | MS.gene60395 | 0.817488 | 3.21E-52 | -1.69E-46 |
| MS.gene049222 | MS.gene60395 | 0.843216 | 1.61E-58 | -1.69E-46 |
| MS.gene049285 | MS.gene60395 | 0.804929 | 1.71E-49 | -1.69E-46 |
| MS.gene049320 | MS.gene60395 | 0.81279 | 3.55E-51 | -1.69E-46 |
| MS.gene049427 | MS.gene60395 | 0.860579 | 1.87E-63 | -1.69E-46 |
| MS.gene049488 | MS.gene60395 | 0.839699 | 1.36E-57 | -1.69E-46 |
| MS.gene049609 | MS.gene60395 | 0.892629 | 1.33E-74 | -1.69E-46 |
| MS.gene049661 | MS.gene60395 | 0.850335 | 1.82E-60 | -1.69E-46 |
| MS.gene049662 | MS.gene60395 | 0.840824 | 6.89E-58 | -1.69E-46 |
| MS.gene049805 | MS.gene60395 | 0.882378 | 1.10E-70 | -1.69E-46 |
| MS.gene050076 | MS.gene60395 | 0.805532 | 1.28E-49 | -1.69E-46 |
| MS.gene050163 | MS.gene60395 | 0.808706 | 2.72E-50 | -1.69E-46 |
| MS.gene050301 | MS.gene60395 | 0.800622 | 1.32E-48 | -1.69E-46 |
| MS.gene050432 | MS.gene60395 | 0.83311 | 6.44E-56 | -1.69E-46 |
| MS.gene050538 | MS.gene60395 | 0.823938 | 1.06E-53 | -1.69E-46 |
| MS.gene050577 | MS.gene60395 | 0.806816 | 6.85E-50 | -1.69E-46 |
| MS.gene050593 | MS.gene60395 | 0.890913 | 6.40E-74 | -1.69E-46 |
| MS.gene050625 | MS.gene60395 | 0.823768 | 1.16E-53 | -1.69E-46 |
| MS.gene050670 | MS.gene60395 | 0.899697 | 1.53E-77 | -1.69E-46 |
| MS.gene050675 | MS.gene60395 | 0.800977 | 1.12E-48 | -1.69E-46 |
| MS.gene050690 | MS.gene60395 | 0.820089 | 8.24E-53 | -1.69E-46 |
| MS.gene050699 | MS.gene60395 | 0.831916 | 1.27E-55 | -1.69E-46 |
| MS.gene050747 | MS.gene60395 | 0.870016 | 2.01E-66 | -1.69E-46 |
| MS.gene050772 | MS.gene60395 | 0.867089 | 1.77E-65 | -1.69E-46 |
| MS.gene050783 | MS.gene60395 | 0.819222 | 1.30E-52 | -1.69E-46 |
| MS.gene05080 | MS.gene60395 | 0.806047 | 9.95E-50 | -1.69E-46 |
| MS.gene050950 | MS.gene60395 | 0.868422 | 6.61E-66 | -1.69E-46 |
| MS.gene05101 | MS.gene60395 | 0.82087 | 5.46E-53 | -1.69E-46 |
| MS.gene05105 | MS.gene60395 | 0.897196 | 1.78E-76 | -1.69E-46 |
| MS.gene051387 | MS.gene60395 | 0.88113 | 3.10E-70 | -1.69E-46 |
| MS.gene051525 | MS.gene60395 | 0.830107 | 3.54E-55 | -1.69E-46 |
| MS.gene051582 | MS.gene60395 | 0.862458 | 5.00E-64 | -1.69E-46 |
| MS.gene051666 | MS.gene60395 | 0.872347 | 3.41E-67 | -1.69E-46 |
| MS.gene051692 | MS.gene60395 | 0.81877 | 1.65E-52 | -1.69E-46 |
| MS.gene051694 | MS.gene60395 | 0.819652 | 1.04E-52 | -1.69E-46 |
| MS.gene051838 | MS.gene60395 | 0.83499 | 2.18E-56 | -1.69E-46 |
| MS.gene051858 | MS.gene60395 | 0.821695 | 3.52E-53 | -1.69E-46 |
| MS.gene052011 | MS.gene60395 | -0.80338 | 3.58E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene60395 | 0.80266 | 5.05E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene60395 | 0.817778 | 2.76E-52 | -1.69E-46 |
| MS.gene052253 | MS.gene60395 | 0.8504 | 1.74E-60 | -1.69E-46 |
| MS.gene052310 | MS.gene60395 | 0.844947 | 5.51E-59 | -1.69E-46 |
| MS.gene052322 | MS.gene60395 | 0.855957 | 4.45E-62 | -1.69E-46 |
| MS.gene052340 | MS.gene60395 | 0.80898 | 2.37E-50 | -1.69E-46 |
| MS.gene052379 | MS.gene60395 | 0.804996 | 1.65E-49 | -1.69E-46 |
| MS.gene052414 | MS.gene60395 | 0.813723 | 2.22E-51 | -1.69E-46 |
| MS.gene052421 | MS.gene60395 | 0.878466 | 2.75E-69 | -1.69E-46 |
| MS.gene052481 | MS.gene60395 | 0.887816 | 1.02E-72 | -1.69E-46 |
| MS.gene052484 | MS.gene60395 | 0.860791 | 1.61E-63 | -1.69E-46 |
| MS.gene052485 | MS.gene60395 | 0.840399 | 8.90E-58 | -1.69E-46 |
| MS.gene052501 | MS.gene60395 | 0.886158 | 4.36E-72 | -1.69E-46 |
| MS.gene052503 | MS.gene60395 | 0.846148 | 2.61E-59 | -1.69E-46 |
| MS.gene052505 | MS.gene60395 | 0.885948 | 5.23E-72 | -1.69E-46 |
| MS.gene052615 | MS.gene60395 | 0.874891 | 4.74E-68 | -1.69E-46 |
| MS.gene052823 | MS.gene60395 | 0.835044 | 2.11E-56 | -1.69E-46 |
| MS.gene052825 | MS.gene60395 | 0.835468 | 1.65E-56 | -1.69E-46 |
| MS.gene052982 | MS.gene60395 | 0.871703 | 5.59E-67 | -1.69E-46 |
| MS.gene053009 | MS.gene60395 | 0.809917 | 1.49E-50 | -1.69E-46 |
| MS.gene053103 | MS.gene60395 | 0.831756 | 1.39E-55 | -1.69E-46 |
| MS.gene053104 | MS.gene60395 | 0.818045 | 2.41E-52 | -1.69E-46 |
| MS.gene053107 | MS.gene60395 | 0.810217 | 1.29E-50 | -1.69E-46 |
| MS.gene053203 | MS.gene60395 | 0.808885 | 2.49E-50 | -1.69E-46 |
| MS.gene053207 | MS.gene60395 | 0.860148 | 2.53E-63 | -1.69E-46 |
| MS.gene053209 | MS.gene60395 | 0.824008 | 1.02E-53 | -1.69E-46 |
| MS.gene053213 | MS.gene60395 | 0.872182 | 3.87E-67 | -1.69E-46 |
| MS.gene053286 | MS.gene60395 | 0.855118 | 7.82E-62 | -1.69E-46 |
| MS.gene053322 | MS.gene60395 | 0.855203 | 7.38E-62 | -1.69E-46 |
| MS.gene053436 | MS.gene60395 | 0.845199 | 4.71E-59 | -1.69E-46 |
| MS.gene053574 | MS.gene60395 | 0.864493 | 1.17E-64 | -1.69E-46 |
| MS.gene053579 | MS.gene60395 | 0.834501 | 2.89E-56 | -1.69E-46 |
| MS.gene053632 | MS.gene60395 | 0.864664 | 1.03E-64 | -1.69E-46 |
| MS.gene053873 | MS.gene60395 | 0.82729 | 1.70E-54 | -1.69E-46 |
| MS.gene053912 | MS.gene60395 | 0.827944 | 1.18E-54 | -1.69E-46 |
| MS.gene054408 | MS.gene60395 | 0.819739 | 9.91E-53 | -1.69E-46 |
| MS.gene054600 | MS.gene60395 | 0.823087 | 1.67E-53 | -1.69E-46 |
| MS.gene054642 | MS.gene60395 | 0.828018 | 1.14E-54 | -1.69E-46 |
| MS.gene054742 | MS.gene60395 | -0.806364 | 8.53E-50 | -1.69E-46 |
| MS.gene054884 | MS.gene60395 | -0.811823 | 5.77E-51 | -1.69E-46 |
| MS.gene054927 | MS.gene60395 | 0.884565 | 1.72E-71 | -1.69E-46 |
| MS.gene05505 | MS.gene60395 | 0.855242 | 7.20E-62 | -1.69E-46 |
| MS.gene055116 | MS.gene60395 | 0.874929 | 4.60E-68 | -1.69E-46 |
| MS.gene055206 | MS.gene60395 | 0.847308 | 1.26E-59 | -1.69E-46 |
| MS.gene055289 | MS.gene60395 | 0.847885 | 8.71E-60 | -1.69E-46 |
| MS.gene055362 | MS.gene60395 | 0.814014 | 1.91E-51 | -1.69E-46 |
| MS.gene055369 | MS.gene60395 | 0.816006 | 6.91E-52 | -1.69E-46 |
| MS.gene055370 | MS.gene60395 | 0.802738 | 4.87E-49 | -1.69E-46 |
| MS.gene055412 | MS.gene60395 | 0.832914 | 7.20E-56 | -1.69E-46 |
| MS.gene055486 | MS.gene60395 | 0.817604 | 3.03E-52 | -1.69E-46 |
| MS.gene055499 | MS.gene60395 | 0.846282 | 2.39E-59 | -1.69E-46 |
| MS.gene055502 | MS.gene60395 | 0.823102 | 1.66E-53 | -1.69E-46 |
| MS.gene05553 | MS.gene60395 | 0.805972 | 1.03E-49 | -1.69E-46 |
| MS.gene055577 | MS.gene60395 | 0.815075 | 1.11E-51 | -1.69E-46 |
| MS.gene055647 | MS.gene60395 | 0.826224 | 3.05E-54 | -1.69E-46 |
| MS.gene055722 | MS.gene60395 | 0.829083 | 6.28E-55 | -1.69E-46 |
| MS.gene05592 | MS.gene60395 | 0.838029 | 3.66E-57 | -1.69E-46 |
| MS.gene055941 | MS.gene60395 | 0.829592 | 4.72E-55 | -1.69E-46 |
| MS.gene05594 | MS.gene60395 | 0.818874 | 1.56E-52 | -1.69E-46 |
| MS.gene056040 | MS.gene60395 | 0.825567 | 4.37E-54 | -1.69E-46 |
| MS.gene056083 | MS.gene60395 | 0.835873 | 1.30E-56 | -1.69E-46 |
| MS.gene056166 | MS.gene60395 | 0.817683 | 2.90E-52 | -1.69E-46 |
| MS.gene056191 | MS.gene60395 | 0.816865 | 4.44E-52 | -1.69E-46 |
| MS.gene056337 | MS.gene60395 | 0.823644 | 1.24E-53 | -1.69E-46 |
| MS.gene056374 | MS.gene60395 | 0.822971 | 1.78E-53 | -1.69E-46 |
| MS.gene056407 | MS.gene60395 | 0.850861 | 1.29E-60 | -1.69E-46 |
| MS.gene056497 | MS.gene60395 | 0.803378 | 3.59E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene60395 | 0.869 | 4.30E-66 | -1.69E-46 |
| MS.gene056514 | MS.gene60395 | 0.833429 | 5.36E-56 | -1.69E-46 |
| MS.gene05664 | MS.gene60395 | 0.832919 | 7.18E-56 | -1.69E-46 |
| MS.gene056657 | MS.gene60395 | 0.804089 | 2.55E-49 | -1.69E-46 |
| MS.gene056659 | MS.gene60395 | 0.803149 | 4.00E-49 | -1.69E-46 |
| MS.gene05666 | MS.gene60395 | 0.820417 | 6.94E-53 | -1.69E-46 |
| MS.gene056782 | MS.gene60395 | 0.813037 | 3.14E-51 | -1.69E-46 |
| MS.gene056877 | MS.gene60395 | 0.810378 | 1.19E-50 | -1.69E-46 |
| MS.gene057011 | MS.gene60395 | 0.838839 | 2.27E-57 | -1.69E-46 |
| MS.gene05702 | MS.gene60395 | 0.843121 | 1.70E-58 | -1.69E-46 |
| MS.gene057048 | MS.gene60395 | 0.820103 | 8.18E-53 | -1.69E-46 |
| MS.gene057076 | MS.gene60395 | 0.850238 | 1.93E-60 | -1.69E-46 |
| MS.gene057123 | MS.gene60395 | 0.824232 | 9.02E-54 | -1.69E-46 |
| MS.gene057124 | MS.gene60395 | 0.875026 | 4.26E-68 | -1.69E-46 |
| MS.gene057129 | MS.gene60395 | 0.844657 | 6.60E-59 | -1.69E-46 |
| MS.gene057146 | MS.gene60395 | 0.876988 | 9.01E-69 | -1.69E-46 |
| MS.gene057196 | MS.gene60395 | 0.814139 | 1.79E-51 | -1.69E-46 |
| MS.gene057310 | MS.gene60395 | 0.804354 | 2.25E-49 | -1.69E-46 |
| MS.gene057331 | MS.gene60395 | 0.845367 | 4.25E-59 | -1.69E-46 |
| MS.gene057332 | MS.gene60395 | 0.810249 | 1.27E-50 | -1.69E-46 |
| MS.gene057416 | MS.gene60395 | 0.891315 | 4.44E-74 | -1.69E-46 |
| MS.gene057476 | MS.gene60395 | 0.804794 | 1.82E-49 | -1.69E-46 |
| MS.gene057658 | MS.gene60395 | 0.832273 | 1.04E-55 | -1.69E-46 |
| MS.gene057734 | MS.gene60395 | 0.842449 | 2.57E-58 | -1.69E-46 |
| MS.gene057749 | MS.gene60395 | 0.846544 | 2.03E-59 | -1.69E-46 |
| MS.gene057823 | MS.gene60395 | 0.842737 | 2.15E-58 | -1.69E-46 |
| MS.gene057825 | MS.gene60395 | 0.806338 | 8.64E-50 | -1.69E-46 |
| MS.gene057831 | MS.gene60395 | 0.812047 | 5.16E-51 | -1.69E-46 |
| MS.gene057856 | MS.gene60395 | 0.810329 | 1.22E-50 | -1.69E-46 |
| MS.gene058006 | MS.gene60395 | 0.834487 | 2.91E-56 | -1.69E-46 |
| MS.gene058007 | MS.gene60395 | 0.834542 | 2.82E-56 | -1.69E-46 |
| MS.gene058019 | MS.gene60395 | 0.870468 | 1.43E-66 | -1.69E-46 |
| MS.gene058130 | MS.gene60395 | 0.834423 | 3.02E-56 | -1.69E-46 |
| MS.gene058233 | MS.gene60395 | 0.862205 | 5.97E-64 | -1.69E-46 |
| MS.gene058293 | MS.gene60395 | 0.808333 | 3.26E-50 | -1.69E-46 |
| MS.gene058376 | MS.gene60395 | 0.82042 | 6.92E-53 | -1.69E-46 |
| MS.gene05837 | MS.gene60395 | 0.806179 | 9.33E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene60395 | 0.841468 | 4.67E-58 | -1.69E-46 |
| MS.gene058444 | MS.gene60395 | 0.817926 | 2.56E-52 | -1.69E-46 |
| MS.gene058544 | MS.gene60395 | 0.859441 | 4.13E-63 | -1.69E-46 |
| MS.gene058660 | MS.gene60395 | 0.851754 | 7.22E-61 | -1.69E-46 |
| MS.gene058700 | MS.gene60395 | 0.843911 | 1.05E-58 | -1.69E-46 |
| MS.gene058723 | MS.gene60395 | 0.801282 | 9.69E-49 | -1.69E-46 |
| MS.gene058838 | MS.gene60395 | 0.832592 | 8.66E-56 | -1.69E-46 |
| MS.gene05883 | MS.gene60395 | 0.856162 | 3.88E-62 | -1.69E-46 |
| MS.gene05886 | MS.gene60395 | 0.835645 | 1.49E-56 | -1.69E-46 |
| MS.gene058928 | MS.gene60395 | 0.814602 | 1.42E-51 | -1.69E-46 |
| MS.gene059011 | MS.gene60395 | 0.805296 | 1.43E-49 | -1.69E-46 |
| MS.gene059023 | MS.gene60395 | 0.828935 | 6.82E-55 | -1.69E-46 |
| MS.gene059141 | MS.gene60395 | 0.856083 | 4.09E-62 | -1.69E-46 |
| MS.gene05934 | MS.gene60395 | 0.839328 | 1.69E-57 | -1.69E-46 |
| MS.gene059420 | MS.gene60395 | 0.826568 | 2.53E-54 | -1.69E-46 |
| MS.gene059455 | MS.gene60395 | 0.837377 | 5.39E-57 | -1.69E-46 |
| MS.gene059536 | MS.gene60395 | 0.866949 | 1.96E-65 | -1.69E-46 |
| MS.gene059676 | MS.gene60395 | 0.820444 | 6.84E-53 | -1.69E-46 |
| MS.gene059677 | MS.gene60395 | 0.81738 | 3.40E-52 | -1.69E-46 |
| MS.gene059688 | MS.gene60395 | 0.884078 | 2.61E-71 | -1.69E-46 |
| MS.gene059692 | MS.gene60395 | 0.871757 | 5.36E-67 | -1.69E-46 |
| MS.gene059695 | MS.gene60395 | 0.875192 | 3.74E-68 | -1.69E-46 |
| MS.gene059718 | MS.gene60395 | 0.846801 | 1.73E-59 | -1.69E-46 |
| MS.gene059756 | MS.gene60395 | 0.810181 | 1.31E-50 | -1.69E-46 |
| MS.gene059902 | MS.gene60395 | 0.821365 | 4.20E-53 | -1.69E-46 |
| MS.gene059960 | MS.gene60395 | 0.827765 | 1.31E-54 | -1.69E-46 |
| MS.gene060403 | MS.gene60395 | 0.88408 | 2.60E-71 | -1.69E-46 |
| MS.gene060485 | MS.gene60395 | 0.811939 | 5.45E-51 | -1.69E-46 |
| MS.gene060804 | MS.gene60395 | 0.846126 | 2.64E-59 | -1.69E-46 |
| MS.gene060835 | MS.gene60395 | 0.846342 | 2.31E-59 | -1.69E-46 |
| MS.gene060837 | MS.gene60395 | 0.829146 | 6.06E-55 | -1.69E-46 |
| MS.gene06092 | MS.gene60395 | 0.871441 | 6.82E-67 | -1.69E-46 |
| MS.gene061041 | MS.gene60395 | 0.811341 | 7.35E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene60395 | 0.820032 | 8.50E-53 | -1.69E-46 |
| MS.gene061150 | MS.gene60395 | 0.826171 | 3.14E-54 | -1.69E-46 |
| MS.gene061154 | MS.gene60395 | 0.805007 | 1.64E-49 | -1.69E-46 |
| MS.gene061200 | MS.gene60395 | 0.836174 | 1.09E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60395.t1 | MTR_4g098620 | 99.127 | 229 | 2 | 0 | 1 | 229 | 1 | 229 | 1.52e-171 | 471 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60395.t1 | AT1G32520 | 73.640 | 239 | 53 | 7 | 1 | 229 | 1 | 239 | 6.47e-121 | 343 |
Find 52 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTCGTGGTGGGCCGCAATT+TGG | 0.091289 | 8.1:-24175815 | MS.gene60395:CDS |
| ACAAGTCCATATTGTTTATT+TGG | 0.118956 | 8.1:-24176569 | MS.gene60395:CDS |
| CTAGTATAAGCTTGCAATTT+TGG | 0.186690 | 8.1:+24175589 | None:intergenic |
| CTCGTGGTGGGCCGCAATTT+GGG | 0.206328 | 8.1:-24175814 | MS.gene60395:CDS |
| CCAACCTTAGGCAAAATTAT+TGG | 0.222718 | 8.1:+24175866 | None:intergenic |
| GCTGGGCCTGATACTAATTC+TGG | 0.242393 | 8.1:-24175740 | MS.gene60395:CDS |
| CTCCTTGCCATCTTCCCTTT+TGG | 0.242724 | 8.1:+24175670 | None:intergenic |
| AAAGGGCCATGTGTAATAAT+AGG | 0.265542 | 8.1:-24176007 | MS.gene60395:CDS |
| CCAGCAAAACCACCCATAAC+TGG | 0.267578 | 8.1:+24175758 | None:intergenic |
| TCTGTGCTTCTATATCCTTC+AGG | 0.271435 | 8.1:+24175947 | None:intergenic |
| CACCCATAACTGGGGCTAAT+GGG | 0.279555 | 8.1:+24175768 | None:intergenic |
| GGGCTGATGGGCTTCTCATC+GGG | 0.321712 | 8.1:-24175793 | MS.gene60395:CDS |
| TTCCAACCAAATAAACAATA+TGG | 0.321938 | 8.1:+24176563 | None:intergenic |
| CCACCCATAACTGGGGCTAA+TGG | 0.338213 | 8.1:+24175767 | None:intergenic |
| ACAGACAACTCTTTCAAGTT+TGG | 0.342835 | 8.1:-24175980 | MS.gene60395:CDS |
| GGGCCGCAATTTGGGGCTGA+TGG | 0.348553 | 8.1:-24175806 | MS.gene60395:CDS |
| GGGGCTGATGGGCTTCTCAT+CGG | 0.364017 | 8.1:-24175794 | MS.gene60395:CDS |
| CTGTGCTTCTATATCCTTCA+GGG | 0.408081 | 8.1:+24175948 | None:intergenic |
| GTCCATATTGTTTATTTGGT+TGG | 0.412950 | 8.1:-24176565 | MS.gene60395:CDS |
| GAGACAAGCCAAGTCTAGAT+TGG | 0.419344 | 8.1:-24175705 | MS.gene60395:CDS |
| TTGCCTAAGGTTGGTGGCAG+TGG | 0.427431 | 8.1:-24175857 | MS.gene60395:CDS |
| TGCTACAGGGCAACAATTGA+TGG | 0.444287 | 8.1:-24176067 | MS.gene60395:CDS |
| GGCCGCAATTTGGGGCTGAT+GGG | 0.455119 | 8.1:-24175805 | MS.gene60395:CDS |
| TGTTTATTTGGTTGGAACAT+AGG | 0.460604 | 8.1:-24176557 | MS.gene60395:CDS |
| AGACAAGCCAAGTCTAGATT+GGG | 0.460900 | 8.1:-24175704 | MS.gene60395:CDS |
| AGGAACTGAAATGCTGCTAC+AGG | 0.463256 | 8.1:-24176081 | MS.gene60395:CDS |
| TGGGATTGTCTTATGCCAAA+AGG | 0.463760 | 8.1:-24175685 | MS.gene60395:CDS |
| GCACCACTGCCACCAACCTT+AGG | 0.472228 | 8.1:+24175854 | None:intergenic |
| CAGCAAAACCACCCATAACT+GGG | 0.479885 | 8.1:+24175759 | None:intergenic |
| CCAACACCAGAATTAGTATC+AGG | 0.485918 | 8.1:+24175734 | None:intergenic |
| GGGATTGTCTTATGCCAAAA+GGG | 0.500151 | 8.1:-24175684 | MS.gene60395:CDS |
| ACCCATAACTGGGGCTAATG+GGG | 0.505857 | 8.1:+24175769 | None:intergenic |
| CTTTAAAATCACAACATTGG+TGG | 0.526175 | 8.1:+24176026 | None:intergenic |
| CAAATTCTCTATCGTTCCAA+TGG | 0.526696 | 8.1:-24176598 | MS.gene60395:CDS |
| TCGTGGTGGGCCGCAATTTG+GGG | 0.534768 | 8.1:-24175813 | MS.gene60395:CDS |
| TCTTATGCCAAAAGGGAAGA+TGG | 0.539221 | 8.1:-24175677 | MS.gene60395:CDS |
| TTTGGTGCATTTAACCCTGA+AGG | 0.542646 | 8.1:-24175962 | MS.gene60395:CDS |
| CTGTTTGATTATGCTCGTGG+TGG | 0.546126 | 8.1:-24175827 | MS.gene60395:CDS |
| CCTGATACTAATTCTGGTGT+TGG | 0.548121 | 8.1:-24175734 | MS.gene60395:CDS |
| GCCCTTTAAAATCACAACAT+TGG | 0.553032 | 8.1:+24176023 | None:intergenic |
| ATATCATGATATTTAATCTG+AGG | 0.554912 | 8.1:+24176512 | None:intergenic |
| TGCCAAAAGGGAAGATGGCA+AGG | 0.559565 | 8.1:-24175672 | MS.gene60395:CDS |
| CCATTAGCCCCAGTTATGGG+TGG | 0.560552 | 8.1:-24175767 | MS.gene60395:CDS |
| AGCCCATCAGCCCCAAATTG+CGG | 0.561376 | 8.1:+24175803 | None:intergenic |
| TGTTTGATTATGCTCGTGGT+GGG | 0.570159 | 8.1:-24175826 | MS.gene60395:CDS |
| ATTCTCTATCGTTCCAATGG+AGG | 0.581526 | 8.1:-24176595 | MS.gene60395:CDS |
| AGACAATCCCAATCTAGACT+TGG | 0.587589 | 8.1:+24175697 | None:intergenic |
| CCCATAACTGGGGCTAATGG+GGG | 0.595547 | 8.1:+24175770 | None:intergenic |
| GCTCTGTTTGATTATGCTCG+TGG | 0.617447 | 8.1:-24175830 | MS.gene60395:CDS |
| GGAACTGAAATGCTGCTACA+GGG | 0.624888 | 8.1:-24176080 | MS.gene60395:CDS |
| GTGTAACCTATTATTACACA+TGG | 0.631481 | 8.1:+24176001 | None:intergenic |
| AGCAAAACCACCCATAACTG+GGG | 0.696608 | 8.1:+24175760 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATACACAATAAAAAAAT+CGG | + | chr8.1:24175861-24175880 | None:intergenic | 10.0% |
| !!! | TTTAAATTTGAAAAAGTATC+TGG | - | chr8.1:24175949-24175968 | MS.gene60395:CDS | 15.0% |
| !! | AATTCCAAAACTAATTTAGT+CGG | + | chr8.1:24175903-24175922 | None:intergenic | 20.0% |
| !! | ATATCATGATATTTAATCTG+AGG | + | chr8.1:24175694-24175713 | None:intergenic | 20.0% |
| !! | CAATAAAAAAATCGGATAAA+CGG | + | chr8.1:24175853-24175872 | None:intergenic | 20.0% |
| !! | TATTTAATGTTATCATCCTT+AGG | - | chr8.1:24176076-24176095 | MS.gene60395:CDS | 20.0% |
| !! | TGAATATGTTACTACTATTT+AGG | - | chr8.1:24175824-24175843 | MS.gene60395:CDS | 20.0% |
| !!! | GTTAACAAAACATTTTTGAA+AGG | - | chr8.1:24175739-24175758 | MS.gene60395:CDS | 20.0% |
| !!! | GTTATATCAGTTTATCATTT+TGG | + | chr8.1:24176053-24176072 | None:intergenic | 20.0% |
| !!! | TGATGCATTTCTATTTTATT+GGG | - | chr8.1:24176297-24176316 | MS.gene60395:intron | 20.0% |
| !!! | TTGATGCATTTCTATTTTAT+TGG | - | chr8.1:24176296-24176315 | MS.gene60395:intron | 20.0% |
| ! | AAATCTACACCATAATTAAG+AGG | + | chr8.1:24175926-24175945 | None:intergenic | 25.0% |
| ! | ACAAGTCCATATTGTTTATT+TGG | - | chr8.1:24175634-24175653 | MS.gene60395:CDS | 25.0% |
| ! | TTCCAACCAAATAAACAATA+TGG | + | chr8.1:24175643-24175662 | None:intergenic | 25.0% |
| !! | ACCAATGTTGTGATTTTAAA+GGG | - | chr8.1:24176179-24176198 | MS.gene60395:intron | 25.0% |
| !! | TAGTATAAGCTTGCAATTTT+GGG | + | chr8.1:24176616-24176635 | None:intergenic | 25.0% |
| !! | TTTGGAATTCCTCTTAATTA+TGG | - | chr8.1:24175914-24175933 | MS.gene60395:CDS | 25.0% |
| GTCCATATTGTTTATTTGGT+TGG | - | chr8.1:24175638-24175657 | MS.gene60395:CDS | 30.0% | |
| GTGTAACCTATTATTACACA+TGG | + | chr8.1:24176205-24176224 | None:intergenic | 30.0% | |
| TGTTTATTTGGTTGGAACAT+AGG | - | chr8.1:24175646-24175665 | MS.gene60395:CDS | 30.0% | |
| ! | AGTATAAGCTTGCAATTTTG+GGG | + | chr8.1:24176615-24176634 | None:intergenic | 30.0% |
| ! | CACCAATGTTGTGATTTTAA+AGG | - | chr8.1:24176178-24176197 | MS.gene60395:intron | 30.0% |
| ! | CTAGTATAAGCTTGCAATTT+TGG | + | chr8.1:24176617-24176636 | None:intergenic | 30.0% |
| ! | TGAACCAATAATTTTGCCTA+AGG | - | chr8.1:24176333-24176352 | MS.gene60395:intron | 30.0% |
| !! | CTTTAAAATCACAACATTGG+TGG | + | chr8.1:24176180-24176199 | None:intergenic | 30.0% |
| !! | GTATCCGACTAAATTAGTTT+TGG | - | chr8.1:24175896-24175915 | MS.gene60395:CDS | 30.0% |
| !!! | TATTTTTGGTGATGAGTCTA+GGG | - | chr8.1:24176558-24176577 | MS.gene60395:CDS | 30.0% |
| AAAGGGCCATGTGTAATAAT+AGG | - | chr8.1:24176196-24176215 | MS.gene60395:intron | 35.0% | |
| CAAATTCTCTATCGTTCCAA+TGG | - | chr8.1:24175605-24175624 | MS.gene60395:CDS | 35.0% | |
| CCAACCTTAGGCAAAATTAT+TGG | + | chr8.1:24176340-24176359 | None:intergenic | 35.0% | |
| TCAAAACCAGTTAAAGCCTA+AGG | + | chr8.1:24176095-24176114 | None:intergenic | 35.0% | |
| ! | ATAATTTTGCCTAAGGTTGG+TGG | - | chr8.1:24176340-24176359 | MS.gene60395:intron | 35.0% |
| ! | CCAATAATTTTGCCTAAGGT+TGG | - | chr8.1:24176337-24176356 | MS.gene60395:intron | 35.0% |
| ! | TATCATCCTTAGGCTTTAAC+TGG | - | chr8.1:24176086-24176105 | MS.gene60395:CDS | 35.0% |
| !! | ACAGACAACTCTTTCAAGTT+TGG | - | chr8.1:24176223-24176242 | MS.gene60395:intron | 35.0% |
| !! | GCCCTTTAAAATCACAACAT+TGG | + | chr8.1:24176183-24176202 | None:intergenic | 35.0% |
| !!! | CTATTTTTGGTGATGAGTCT+AGG | - | chr8.1:24176557-24176576 | MS.gene60395:CDS | 35.0% |
| !!! | TAACTGGTTTTGATCAGGTA+AGG | - | chr8.1:24176102-24176121 | MS.gene60395:intron | 35.0% |
| AGACAAGCCAAGTCTAGATT+GGG | - | chr8.1:24176499-24176518 | MS.gene60395:CDS | 40.0% | |
| AGACAATCCCAATCTAGACT+TGG | + | chr8.1:24176509-24176528 | None:intergenic | 40.0% | |
| ATTCTCTATCGTTCCAATGG+AGG | - | chr8.1:24175608-24175627 | MS.gene60395:CDS | 40.0% | |
| CCAACACCAGAATTAGTATC+AGG | + | chr8.1:24176472-24176491 | None:intergenic | 40.0% | |
| CCTGATACTAATTCTGGTGT+TGG | - | chr8.1:24176469-24176488 | MS.gene60395:CDS | 40.0% | |
| CTGTGCTTCTATATCCTTCA+GGG | + | chr8.1:24176258-24176277 | None:intergenic | 40.0% | |
| GGGATTGTCTTATGCCAAAA+GGG | - | chr8.1:24176519-24176538 | MS.gene60395:CDS | 40.0% | |
| TCTGTGCTTCTATATCCTTC+AGG | + | chr8.1:24176259-24176278 | None:intergenic | 40.0% | |
| TCTTATGCCAAAAGGGAAGA+TGG | - | chr8.1:24176526-24176545 | MS.gene60395:CDS | 40.0% | |
| TGGGATTGTCTTATGCCAAA+AGG | - | chr8.1:24176518-24176537 | MS.gene60395:CDS | 40.0% | |
| TGTTTGATTATGCTCGTGGT+GGG | - | chr8.1:24176377-24176396 | MS.gene60395:intron | 40.0% | |
| ! | GATGGCAAGGAGTCTATTTT+TGG | - | chr8.1:24176544-24176563 | MS.gene60395:CDS | 40.0% |
| ! | TTTGGTGCATTTAACCCTGA+AGG | - | chr8.1:24176241-24176260 | MS.gene60395:intron | 40.0% |
| !! | ATGGACTTGTTTTCCTCCAT+TGG | + | chr8.1:24175624-24175643 | None:intergenic | 40.0% |
| !!! | GGCTTTAACTGGTTTTGATC+AGG | - | chr8.1:24176097-24176116 | MS.gene60395:intron | 40.0% |
| AGCAAAACCACCCATAACTG+GGG | + | chr8.1:24176446-24176465 | None:intergenic | 45.0% | |
| AGGAACTGAAATGCTGCTAC+AGG | - | chr8.1:24176122-24176141 | MS.gene60395:intron | 45.0% | |
| CAGCAAAACCACCCATAACT+GGG | + | chr8.1:24176447-24176466 | None:intergenic | 45.0% | |
| CTGTTTGATTATGCTCGTGG+TGG | - | chr8.1:24176376-24176395 | MS.gene60395:intron | 45.0% | |
| GAGACAAGCCAAGTCTAGAT+TGG | - | chr8.1:24176498-24176517 | MS.gene60395:CDS | 45.0% | |
| GCTCTGTTTGATTATGCTCG+TGG | - | chr8.1:24176373-24176392 | MS.gene60395:intron | 45.0% | |
| GGAACTGAAATGCTGCTACA+GGG | - | chr8.1:24176123-24176142 | MS.gene60395:intron | 45.0% | |
| TGCTACAGGGCAACAATTGA+TGG | - | chr8.1:24176136-24176155 | MS.gene60395:intron | 45.0% | |
| !! | CAGTTATGGGTGGTTTTGCT+GGG | - | chr8.1:24176446-24176465 | MS.gene60395:intron | 45.0% |
| ACCCATAACTGGGGCTAATG+GGG | + | chr8.1:24176437-24176456 | None:intergenic | 50.0% | |
| CACCCATAACTGGGGCTAAT+GGG | + | chr8.1:24176438-24176457 | None:intergenic | 50.0% | |
| CCAGCAAAACCACCCATAAC+TGG | + | chr8.1:24176448-24176467 | None:intergenic | 50.0% | |
| GCTGGGCCTGATACTAATTC+TGG | - | chr8.1:24176463-24176482 | MS.gene60395:intron | 50.0% | |
| TGCCAAAAGGGAAGATGGCA+AGG | - | chr8.1:24176531-24176550 | MS.gene60395:CDS | 50.0% | |
| ! | CTCCTTGCCATCTTCCCTTT+TGG | + | chr8.1:24176536-24176555 | None:intergenic | 50.0% |
| !! | CCAGTTATGGGTGGTTTTGC+TGG | - | chr8.1:24176445-24176464 | MS.gene60395:intron | 50.0% |
| AGCCCATCAGCCCCAAATTG+CGG | + | chr8.1:24176403-24176422 | None:intergenic | 55.0% | |
| CCACCCATAACTGGGGCTAA+TGG | + | chr8.1:24176439-24176458 | None:intergenic | 55.0% | |
| CCATTAGCCCCAGTTATGGG+TGG | - | chr8.1:24176436-24176455 | MS.gene60395:intron | 55.0% | |
| CCCATAACTGGGGCTAATGG+GGG | + | chr8.1:24176436-24176455 | None:intergenic | 55.0% | |
| CCCCCATTAGCCCCAGTTAT+GGG | - | chr8.1:24176433-24176452 | MS.gene60395:intron | 55.0% | |
| !! | TTGCCTAAGGTTGGTGGCAG+TGG | - | chr8.1:24176346-24176365 | MS.gene60395:intron | 55.0% |
| GCACCACTGCCACCAACCTT+AGG | + | chr8.1:24176352-24176371 | None:intergenic | 60.0% | |
| GCCCCCATTAGCCCCAGTTA+TGG | - | chr8.1:24176432-24176451 | MS.gene60395:intron | 60.0% | |
| ! | GGCCGCAATTTGGGGCTGAT+GGG | - | chr8.1:24176398-24176417 | MS.gene60395:intron | 60.0% |
| !! | CTCGTGGTGGGCCGCAATTT+GGG | - | chr8.1:24176389-24176408 | MS.gene60395:intron | 60.0% |
| !! | GGGCTGATGGGCTTCTCATC+GGG | - | chr8.1:24176410-24176429 | MS.gene60395:intron | 60.0% |
| !! | GGGGCTGATGGGCTTCTCAT+CGG | - | chr8.1:24176409-24176428 | MS.gene60395:intron | 60.0% |
| !! | TCGTGGTGGGCCGCAATTTG+GGG | - | chr8.1:24176390-24176409 | MS.gene60395:intron | 60.0% |
| ! | GGGCCGCAATTTGGGGCTGA+TGG | - | chr8.1:24176397-24176416 | MS.gene60395:intron | 65.0% |
| !! | GCTCGTGGTGGGCCGCAATT+TGG | - | chr8.1:24176388-24176407 | MS.gene60395:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 24175589 | 24176636 | 24175589 | ID=MS.gene60395 |
| chr8.1 | mRNA | 24175589 | 24176636 | 24175589 | ID=MS.gene60395.t1;Parent=MS.gene60395 |
| chr8.1 | exon | 24176465 | 24176636 | 24176465 | ID=MS.gene60395.t1.exon1;Parent=MS.gene60395.t1 |
| chr8.1 | CDS | 24176465 | 24176636 | 24176465 | ID=cds.MS.gene60395.t1;Parent=MS.gene60395.t1 |
| chr8.1 | exon | 24175589 | 24176106 | 24175589 | ID=MS.gene60395.t1.exon2;Parent=MS.gene60395.t1 |
| chr8.1 | CDS | 24175589 | 24176106 | 24175589 | ID=cds.MS.gene60395.t1;Parent=MS.gene60395.t1 |
| Gene Sequence |
| Protein sequence |
