Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85135.t1 | XP_003597708.1 | 86.8 | 273 | 28 | 1 | 1 | 265 | 1 | 273 | 1.10E-108 | 402.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85135.t1 | G7IUN5 | 86.8 | 273 | 28 | 1 | 1 | 265 | 1 | 273 | 8.3e-109 | 402.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene85135 | 0.861089 | 1.31E-63 | -1.69E-46 |
| MS.gene049171 | MS.gene85135 | 0.862711 | 4.18E-64 | -1.69E-46 |
| MS.gene049173 | MS.gene85135 | 0.880255 | 6.38E-70 | -1.69E-46 |
| MS.gene049217 | MS.gene85135 | 0.847675 | 9.95E-60 | -1.69E-46 |
| MS.gene049222 | MS.gene85135 | 0.872352 | 3.40E-67 | -1.69E-46 |
| MS.gene049285 | MS.gene85135 | 0.846448 | 2.16E-59 | -1.69E-46 |
| MS.gene049320 | MS.gene85135 | 0.855407 | 6.44E-62 | -1.69E-46 |
| MS.gene04939 | MS.gene85135 | 0.800117 | 1.67E-48 | -1.69E-46 |
| MS.gene049427 | MS.gene85135 | 0.839908 | 1.20E-57 | -1.69E-46 |
| MS.gene049609 | MS.gene85135 | 0.876935 | 9.40E-69 | -1.69E-46 |
| MS.gene049633 | MS.gene85135 | 0.815166 | 1.06E-51 | -1.69E-46 |
| MS.gene049805 | MS.gene85135 | 0.871436 | 6.85E-67 | -1.69E-46 |
| MS.gene050163 | MS.gene85135 | 0.858139 | 1.01E-62 | -1.69E-46 |
| MS.gene050165 | MS.gene85135 | 0.846883 | 1.64E-59 | -1.69E-46 |
| MS.gene050333 | MS.gene85135 | 0.82488 | 6.35E-54 | -1.69E-46 |
| MS.gene050444 | MS.gene85135 | 0.816992 | 4.16E-52 | -1.69E-46 |
| MS.gene050504 | MS.gene85135 | 0.834677 | 2.61E-56 | -1.69E-46 |
| MS.gene050518 | MS.gene85135 | 0.825368 | 4.87E-54 | -1.69E-46 |
| MS.gene050538 | MS.gene85135 | 0.843489 | 1.36E-58 | -1.69E-46 |
| MS.gene050577 | MS.gene85135 | 0.855731 | 5.18E-62 | -1.69E-46 |
| MS.gene050593 | MS.gene85135 | 0.84102 | 6.12E-58 | -1.69E-46 |
| MS.gene050612 | MS.gene85135 | 0.830932 | 2.22E-55 | -1.69E-46 |
| MS.gene050670 | MS.gene85135 | 0.813502 | 2.48E-51 | -1.69E-46 |
| MS.gene050675 | MS.gene85135 | 0.821462 | 3.99E-53 | -1.69E-46 |
| MS.gene050699 | MS.gene85135 | 0.844733 | 6.30E-59 | -1.69E-46 |
| MS.gene050747 | MS.gene85135 | 0.848182 | 7.21E-60 | -1.69E-46 |
| MS.gene050772 | MS.gene85135 | 0.868033 | 8.82E-66 | -1.69E-46 |
| MS.gene05080 | MS.gene85135 | 0.822513 | 2.28E-53 | -1.69E-46 |
| MS.gene05081 | MS.gene85135 | 0.806004 | 1.02E-49 | -1.69E-46 |
| MS.gene050950 | MS.gene85135 | 0.834549 | 2.81E-56 | -1.69E-46 |
| MS.gene050986 | MS.gene85135 | 0.803893 | 2.81E-49 | -1.69E-46 |
| MS.gene05100 | MS.gene85135 | 0.874442 | 6.73E-68 | -1.69E-46 |
| MS.gene05105 | MS.gene85135 | 0.867635 | 1.18E-65 | -1.69E-46 |
| MS.gene051228 | MS.gene85135 | 0.805125 | 1.55E-49 | -1.69E-46 |
| MS.gene051387 | MS.gene85135 | 0.801871 | 7.34E-49 | -1.69E-46 |
| MS.gene051551 | MS.gene85135 | 0.806952 | 6.41E-50 | -1.69E-46 |
| MS.gene051666 | MS.gene85135 | 0.887399 | 1.48E-72 | -1.69E-46 |
| MS.gene051692 | MS.gene85135 | 0.836512 | 8.96E-57 | -1.69E-46 |
| MS.gene051694 | MS.gene85135 | 0.805514 | 1.29E-49 | -1.69E-46 |
| MS.gene051810 | MS.gene85135 | 0.836852 | 7.34E-57 | -1.69E-46 |
| MS.gene051838 | MS.gene85135 | 0.813891 | 2.03E-51 | -1.69E-46 |
| MS.gene051858 | MS.gene85135 | 0.839739 | 1.32E-57 | -1.69E-46 |
| MS.gene051949 | MS.gene85135 | 0.803966 | 2.71E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene85135 | 0.845866 | 3.11E-59 | -1.69E-46 |
| MS.gene052271 | MS.gene85135 | 0.833089 | 6.51E-56 | -1.69E-46 |
| MS.gene052379 | MS.gene85135 | 0.822781 | 1.97E-53 | -1.69E-46 |
| MS.gene052414 | MS.gene85135 | 0.819118 | 1.37E-52 | -1.69E-46 |
| MS.gene052421 | MS.gene85135 | 0.820001 | 8.64E-53 | -1.69E-46 |
| MS.gene052481 | MS.gene85135 | 0.863756 | 1.98E-64 | -1.69E-46 |
| MS.gene052482 | MS.gene85135 | 0.809023 | 2.32E-50 | -1.69E-46 |
| MS.gene052484 | MS.gene85135 | 0.852834 | 3.56E-61 | -1.69E-46 |
| MS.gene052486 | MS.gene85135 | 0.815326 | 9.79E-52 | -1.69E-46 |
| MS.gene052501 | MS.gene85135 | 0.865246 | 6.79E-65 | -1.69E-46 |
| MS.gene052503 | MS.gene85135 | 0.861364 | 1.08E-63 | -1.69E-46 |
| MS.gene052505 | MS.gene85135 | 0.85558 | 5.73E-62 | -1.69E-46 |
| MS.gene052615 | MS.gene85135 | 0.843449 | 1.39E-58 | -1.69E-46 |
| MS.gene052823 | MS.gene85135 | 0.857849 | 1.23E-62 | -1.69E-46 |
| MS.gene052825 | MS.gene85135 | 0.853066 | 3.05E-61 | -1.69E-46 |
| MS.gene052966 | MS.gene85135 | 0.800796 | 1.22E-48 | -1.69E-46 |
| MS.gene052982 | MS.gene85135 | 0.824468 | 7.94E-54 | -1.69E-46 |
| MS.gene053103 | MS.gene85135 | 0.846214 | 2.50E-59 | -1.69E-46 |
| MS.gene053104 | MS.gene85135 | 0.861376 | 1.07E-63 | -1.69E-46 |
| MS.gene053207 | MS.gene85135 | 0.833733 | 4.50E-56 | -1.69E-46 |
| MS.gene053213 | MS.gene85135 | 0.86968 | 2.58E-66 | -1.69E-46 |
| MS.gene053286 | MS.gene85135 | 0.861766 | 8.15E-64 | -1.69E-46 |
| MS.gene053322 | MS.gene85135 | 0.84102 | 6.12E-58 | -1.69E-46 |
| MS.gene053436 | MS.gene85135 | 0.829286 | 5.60E-55 | -1.69E-46 |
| MS.gene053449 | MS.gene85135 | 0.814857 | 1.24E-51 | -1.69E-46 |
| MS.gene053480 | MS.gene85135 | 0.807556 | 4.77E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene85135 | 0.857897 | 1.19E-62 | -1.69E-46 |
| MS.gene053579 | MS.gene85135 | 0.80951 | 1.83E-50 | -1.69E-46 |
| MS.gene053632 | MS.gene85135 | 0.879664 | 1.04E-69 | -1.69E-46 |
| MS.gene053824 | MS.gene85135 | 0.823006 | 1.75E-53 | -1.69E-46 |
| MS.gene053916 | MS.gene85135 | 0.826893 | 2.11E-54 | -1.69E-46 |
| MS.gene054408 | MS.gene85135 | 0.824975 | 6.03E-54 | -1.69E-46 |
| MS.gene054595 | MS.gene85135 | 0.80731 | 5.38E-50 | -1.69E-46 |
| MS.gene054600 | MS.gene85135 | 0.868059 | 8.65E-66 | -1.69E-46 |
| MS.gene054642 | MS.gene85135 | 0.86076 | 1.65E-63 | -1.69E-46 |
| MS.gene054706 | MS.gene85135 | -0.800229 | 1.59E-48 | -1.69E-46 |
| MS.gene05470 | MS.gene85135 | 0.83261 | 8.57E-56 | -1.69E-46 |
| MS.gene054927 | MS.gene85135 | 0.843026 | 1.80E-58 | -1.69E-46 |
| MS.gene054954 | MS.gene85135 | 0.820969 | 5.18E-53 | -1.69E-46 |
| MS.gene05505 | MS.gene85135 | 0.835672 | 1.46E-56 | -1.69E-46 |
| MS.gene055116 | MS.gene85135 | 0.811364 | 7.26E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene85135 | 0.846492 | 2.10E-59 | -1.69E-46 |
| MS.gene055212 | MS.gene85135 | 0.850354 | 1.79E-60 | -1.69E-46 |
| MS.gene055224 | MS.gene85135 | 0.80009 | 1.70E-48 | -1.69E-46 |
| MS.gene055289 | MS.gene85135 | 0.841878 | 3.64E-58 | -1.69E-46 |
| MS.gene055362 | MS.gene85135 | 0.85743 | 1.64E-62 | -1.69E-46 |
| MS.gene055397 | MS.gene85135 | 0.802084 | 6.64E-49 | -1.69E-46 |
| MS.gene055499 | MS.gene85135 | 0.825632 | 4.22E-54 | -1.69E-46 |
| MS.gene055502 | MS.gene85135 | 0.874088 | 8.88E-68 | -1.69E-46 |
| MS.gene055577 | MS.gene85135 | 0.84774 | 9.55E-60 | -1.69E-46 |
| MS.gene055647 | MS.gene85135 | 0.813587 | 2.37E-51 | -1.69E-46 |
| MS.gene055722 | MS.gene85135 | 0.858535 | 7.71E-63 | -1.69E-46 |
| MS.gene055748 | MS.gene85135 | 0.811867 | 5.65E-51 | -1.69E-46 |
| MS.gene055750 | MS.gene85135 | 0.808066 | 3.72E-50 | -1.69E-46 |
| MS.gene055751 | MS.gene85135 | 0.804311 | 2.30E-49 | -1.69E-46 |
| MS.gene05589 | MS.gene85135 | 0.828766 | 7.49E-55 | -1.69E-46 |
| MS.gene05592 | MS.gene85135 | 0.819707 | 1.01E-52 | -1.69E-46 |
| MS.gene055941 | MS.gene85135 | 0.861198 | 1.21E-63 | -1.69E-46 |
| MS.gene056166 | MS.gene85135 | 0.817755 | 2.80E-52 | -1.69E-46 |
| MS.gene056374 | MS.gene85135 | 0.843138 | 1.68E-58 | -1.69E-46 |
| MS.gene056504 | MS.gene85135 | 0.838146 | 3.42E-57 | -1.69E-46 |
| MS.gene056511 | MS.gene85135 | 0.812996 | 3.20E-51 | -1.69E-46 |
| MS.gene056549 | MS.gene85135 | 0.815973 | 7.02E-52 | -1.69E-46 |
| MS.gene05664 | MS.gene85135 | 0.806351 | 8.58E-50 | -1.69E-46 |
| MS.gene056657 | MS.gene85135 | 0.863832 | 1.88E-64 | -1.69E-46 |
| MS.gene056692 | MS.gene85135 | 0.823107 | 1.65E-53 | -1.69E-46 |
| MS.gene056897 | MS.gene85135 | 0.809783 | 1.60E-50 | -1.69E-46 |
| MS.gene056946 | MS.gene85135 | 0.802912 | 4.48E-49 | -1.69E-46 |
| MS.gene057048 | MS.gene85135 | 0.832672 | 8.27E-56 | -1.69E-46 |
| MS.gene057076 | MS.gene85135 | 0.849294 | 3.55E-60 | -1.69E-46 |
| MS.gene057129 | MS.gene85135 | 0.850103 | 2.11E-60 | -1.69E-46 |
| MS.gene057146 | MS.gene85135 | 0.826107 | 3.25E-54 | -1.69E-46 |
| MS.gene057196 | MS.gene85135 | 0.808809 | 2.58E-50 | -1.69E-46 |
| MS.gene057331 | MS.gene85135 | 0.812451 | 4.21E-51 | -1.69E-46 |
| MS.gene057332 | MS.gene85135 | 0.803626 | 3.19E-49 | -1.69E-46 |
| MS.gene057416 | MS.gene85135 | 0.848134 | 7.44E-60 | -1.69E-46 |
| MS.gene057658 | MS.gene85135 | 0.846493 | 2.10E-59 | -1.69E-46 |
| MS.gene057734 | MS.gene85135 | 0.819748 | 9.87E-53 | -1.69E-46 |
| MS.gene057831 | MS.gene85135 | 0.848092 | 7.64E-60 | -1.69E-46 |
| MS.gene057856 | MS.gene85135 | 0.819283 | 1.26E-52 | -1.69E-46 |
| MS.gene057926 | MS.gene85135 | 0.812453 | 4.21E-51 | -1.69E-46 |
| MS.gene058019 | MS.gene85135 | 0.886534 | 3.15E-72 | -1.69E-46 |
| MS.gene058338 | MS.gene85135 | 0.830829 | 2.36E-55 | -1.69E-46 |
| MS.gene058376 | MS.gene85135 | 0.816952 | 4.24E-52 | -1.69E-46 |
| MS.gene05837 | MS.gene85135 | 0.81435 | 1.61E-51 | -1.69E-46 |
| MS.gene058419 | MS.gene85135 | 0.889951 | 1.53E-73 | -1.69E-46 |
| MS.gene058444 | MS.gene85135 | 0.818565 | 1.83E-52 | -1.69E-46 |
| MS.gene058544 | MS.gene85135 | 0.848283 | 6.76E-60 | -1.69E-46 |
| MS.gene058723 | MS.gene85135 | 0.834081 | 3.68E-56 | -1.69E-46 |
| MS.gene058748 | MS.gene85135 | 0.804506 | 2.09E-49 | -1.69E-46 |
| MS.gene058773 | MS.gene85135 | 0.820051 | 8.41E-53 | -1.69E-46 |
| MS.gene058838 | MS.gene85135 | 0.871032 | 9.30E-67 | -1.69E-46 |
| MS.gene059022 | MS.gene85135 | 0.835577 | 1.55E-56 | -1.69E-46 |
| MS.gene059023 | MS.gene85135 | 0.844851 | 5.85E-59 | -1.69E-46 |
| MS.gene059029 | MS.gene85135 | 0.811763 | 5.95E-51 | -1.69E-46 |
| MS.gene05912 | MS.gene85135 | 0.82527 | 5.14E-54 | -1.69E-46 |
| MS.gene05913 | MS.gene85135 | 0.855301 | 6.92E-62 | -1.69E-46 |
| MS.gene05914 | MS.gene85135 | 0.807154 | 5.81E-50 | -1.69E-46 |
| MS.gene05934 | MS.gene85135 | 0.867362 | 1.45E-65 | -1.69E-46 |
| MS.gene059367 | MS.gene85135 | 0.813302 | 2.74E-51 | -1.69E-46 |
| MS.gene059455 | MS.gene85135 | 0.850344 | 1.80E-60 | -1.69E-46 |
| MS.gene059536 | MS.gene85135 | 0.864649 | 1.04E-64 | -1.69E-46 |
| MS.gene059667 | MS.gene85135 | 0.833345 | 5.62E-56 | -1.69E-46 |
| MS.gene059677 | MS.gene85135 | 0.857044 | 2.13E-62 | -1.69E-46 |
| MS.gene059688 | MS.gene85135 | 0.824704 | 6.99E-54 | -1.69E-46 |
| MS.gene059692 | MS.gene85135 | 0.82022 | 7.70E-53 | -1.69E-46 |
| MS.gene059695 | MS.gene85135 | 0.824933 | 6.17E-54 | -1.69E-46 |
| MS.gene059718 | MS.gene85135 | 0.855699 | 5.30E-62 | -1.69E-46 |
| MS.gene059756 | MS.gene85135 | 0.819388 | 1.19E-52 | -1.69E-46 |
| MS.gene059801 | MS.gene85135 | 0.831804 | 1.36E-55 | -1.69E-46 |
| MS.gene059865 | MS.gene85135 | 0.823849 | 1.11E-53 | -1.69E-46 |
| MS.gene059902 | MS.gene85135 | 0.835663 | 1.47E-56 | -1.69E-46 |
| MS.gene059955 | MS.gene85135 | -0.804788 | 1.83E-49 | -1.69E-46 |
| MS.gene059960 | MS.gene85135 | 0.838406 | 2.93E-57 | -1.69E-46 |
| MS.gene060193 | MS.gene85135 | 0.807249 | 5.54E-50 | -1.69E-46 |
| MS.gene060403 | MS.gene85135 | 0.82678 | 2.25E-54 | -1.69E-46 |
| MS.gene060703 | MS.gene85135 | 0.841728 | 3.99E-58 | -1.69E-46 |
| MS.gene060762 | MS.gene85135 | 0.847179 | 1.36E-59 | -1.69E-46 |
| MS.gene06077 | MS.gene85135 | 0.833514 | 5.11E-56 | -1.69E-46 |
| MS.gene060804 | MS.gene85135 | 0.855707 | 5.27E-62 | -1.69E-46 |
| MS.gene060835 | MS.gene85135 | 0.850351 | 1.80E-60 | -1.69E-46 |
| MS.gene06092 | MS.gene85135 | 0.849221 | 3.72E-60 | -1.69E-46 |
| MS.gene06097 | MS.gene85135 | 0.81149 | 6.82E-51 | -1.69E-46 |
| MS.gene060982 | MS.gene85135 | 0.822037 | 2.93E-53 | -1.69E-46 |
| MS.gene061041 | MS.gene85135 | 0.846564 | 2.01E-59 | -1.69E-46 |
| MS.gene061150 | MS.gene85135 | 0.843486 | 1.36E-58 | -1.69E-46 |
| MS.gene061200 | MS.gene85135 | 0.849525 | 3.06E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85135.t1 | MTR_2g101430 | 89.377 | 273 | 21 | 1 | 1 | 265 | 1 | 273 | 2.87e-177 | 489 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85135.t1 | AT1G51080 | 46.606 | 221 | 107 | 4 | 47 | 259 | 63 | 280 | 1.64e-55 | 180 |
Find 73 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAGACAGAGTAATAGATTT+TGG | 0.202902 | 2.4:-3353175 | MS.gene85135:CDS |
| CGACCTTCTCGGCACTTCTA+AGG | 0.214375 | 2.4:-3352858 | MS.gene85135:CDS |
| TTTGCTCTTAATGTGTTAAA+TGG | 0.235237 | 2.4:-3353001 | MS.gene85135:CDS |
| TCATGTTTACTTTATGTTTC+GGG | 0.243260 | 2.4:-3353290 | MS.gene85135:CDS |
| GTTAGGTTGGGTGTTGGTAT+TGG | 0.265352 | 2.4:+3352793 | None:intergenic |
| ATCATGTTTACTTTATGTTT+CGG | 0.295381 | 2.4:-3353291 | MS.gene85135:CDS |
| TAGCAGGTGGGTGGTTAGGT+TGG | 0.367914 | 2.4:+3352780 | None:intergenic |
| AAAGTTGATTTCGACCTTCT+CGG | 0.376013 | 2.4:-3352869 | MS.gene85135:CDS |
| TAGGATGCTGCCATGGTTGA+TGG | 0.394720 | 2.4:+3352547 | None:intergenic |
| TCAATGCGGTCTTTGTAGAT+TGG | 0.399152 | 2.4:+3353031 | None:intergenic |
| CTTAAGAGCACGCGAGTTTG+AGG | 0.411946 | 2.4:-3353089 | MS.gene85135:CDS |
| CTAGTAGCAGGTGGGTGGTT+AGG | 0.415512 | 2.4:+3352776 | None:intergenic |
| CAATCCATTCCCCGGACGTC+AGG | 0.418797 | 2.4:-3352585 | MS.gene85135:CDS |
| TCTGCTGCTGCTAGATCCAT+TGG | 0.419249 | 2.4:-3352965 | MS.gene85135:CDS |
| CTTTGTAGATTGGGTCTAGA+AGG | 0.419945 | 2.4:+3353041 | None:intergenic |
| CAATGCGGTCTTTGTAGATT+GGG | 0.420162 | 2.4:+3353032 | None:intergenic |
| TTTAAAGTTGAGTTTAGCTT+AGG | 0.423436 | 2.4:+3352528 | None:intergenic |
| AGCAGGTGGGTGGTTAGGTT+GGG | 0.433268 | 2.4:+3352781 | None:intergenic |
| CAGGAGATAAGCAAGAGATT+TGG | 0.434593 | 2.4:-3352923 | MS.gene85135:CDS |
| CAAAATCTATTACTCTGTCT+CGG | 0.440622 | 2.4:+3353176 | None:intergenic |
| TGGGTGGTTAGGTTGGGTGT+TGG | 0.441793 | 2.4:+3352787 | None:intergenic |
| TTCAGAAAGAGAAATTAAAC+TGG | 0.447060 | 2.4:-3353200 | MS.gene85135:CDS |
| AAAATACAAGGGGAAGATGT+TGG | 0.457200 | 2.4:-3353152 | MS.gene85135:CDS |
| CTCAGAATCTGCCGCTCCAA+TGG | 0.465779 | 2.4:+3352949 | None:intergenic |
| ACGGAGAAGAGCCTGACGTC+CGG | 0.467710 | 2.4:+3352574 | None:intergenic |
| AAATCAAAAGCAGCGTTATG+TGG | 0.472830 | 2.4:-3353251 | MS.gene85135:CDS |
| CCACCTGCTACTAGTATCAT+TGG | 0.480418 | 2.4:-3352767 | MS.gene85135:CDS |
| GATAAGCAAGAGATTTGGTT+GGG | 0.481364 | 2.4:-3352918 | MS.gene85135:CDS |
| AGAGCCTGACGTCCGGGGAA+TGG | 0.482518 | 2.4:+3352581 | None:intergenic |
| AAATACAAGGGGAAGATGTT+GGG | 0.482832 | 2.4:-3353151 | MS.gene85135:CDS |
| CGGAGAAGAGCCTGACGTCC+GGG | 0.490784 | 2.4:+3352575 | None:intergenic |
| GACCTTCTCGGCACTTCTAA+GGG | 0.491235 | 2.4:-3352857 | MS.gene85135:CDS |
| CTGACGTCCGGGGAATGGAT+TGG | 0.498352 | 2.4:+3352586 | None:intergenic |
| AAAATCTATTACTCTGTCTC+GGG | 0.505732 | 2.4:+3353177 | None:intergenic |
| AGCGGCAGATTCTGAGCTGC+AGG | 0.508668 | 2.4:-3352942 | MS.gene85135:CDS |
| CCACATTGTTGTCCTTCTGT+TGG | 0.509399 | 2.4:+3352654 | None:intergenic |
| TGCCATGGTTGATGGCCTTA+CGG | 0.514572 | 2.4:+3352555 | None:intergenic |
| TCGCTGAAATTGACAAGTCT+CGG | 0.520757 | 2.4:+3352824 | None:intergenic |
| TTCCGTCGAGCTACCTCAAG+TGG | 0.521226 | 2.4:-3353123 | MS.gene85135:CDS |
| AGCACGCGAGTTTGAGGAGT+GGG | 0.522721 | 2.4:-3353083 | MS.gene85135:CDS |
| ACAGTAGTAGTAGGACTAAG+AGG | 0.528829 | 2.4:-3352730 | MS.gene85135:CDS |
| GCCATTGCCAATCCATTCCC+CGG | 0.538546 | 2.4:-3352593 | MS.gene85135:CDS |
| ACGGTAGTAACAGTAGTAGT+AGG | 0.539912 | 2.4:-3352739 | MS.gene85135:CDS |
| CTCCCTTAGAAGTGCCGAGA+AGG | 0.555291 | 2.4:+3352855 | None:intergenic |
| AATCAAAAGCAGCGTTATGT+GGG | 0.565427 | 2.4:-3353250 | MS.gene85135:CDS |
| TGCTCCAACCAACTTTGTCA+AGG | 0.565623 | 2.4:+3352891 | None:intergenic |
| ACGTCAGGCTCTTCTCCGTA+AGG | 0.566162 | 2.4:-3352570 | MS.gene85135:CDS |
| AAATGAAGCGCACCAACAGA+AGG | 0.567882 | 2.4:-3352666 | MS.gene85135:CDS |
| AGATAAGCAAGAGATTTGGT+TGG | 0.569741 | 2.4:-3352919 | MS.gene85135:CDS |
| TAGTAGTAGGACTAAGAGGA+TGG | 0.572795 | 2.4:-3352726 | MS.gene85135:CDS |
| CTACCAATGATACTAGTAGC+AGG | 0.580274 | 2.4:+3352764 | None:intergenic |
| TCCGGGGAATGGATTGGCAA+TGG | 0.584712 | 2.4:+3352592 | None:intergenic |
| TGGGATAACCTTGACAAAGT+TGG | 0.586673 | 2.4:-3352899 | MS.gene85135:CDS |
| ATAACCTTGACAAAGTTGGT+TGG | 0.591237 | 2.4:-3352895 | MS.gene85135:CDS |
| TTCTCGGCACTTCTAAGGGA+GGG | 0.601983 | 2.4:-3352853 | MS.gene85135:CDS |
| TTTAGCTTAGGATGCTGCCA+TGG | 0.602732 | 2.4:+3352540 | None:intergenic |
| CTCCGTAAGGCCATCAACCA+TGG | 0.607049 | 2.4:-3352557 | MS.gene85135:CDS |
| TCTACAAAGACCGCATTGAG+TGG | 0.608441 | 2.4:-3353027 | MS.gene85135:CDS |
| ACTAGTATCATTGGTAGCAA+CGG | 0.610173 | 2.4:-3352758 | MS.gene85135:CDS |
| TCCGTCGAGCTACCTCAAGT+GGG | 0.610493 | 2.4:-3353122 | MS.gene85135:CDS |
| CCAATGATACTAGTAGCAGG+TGG | 0.627861 | 2.4:+3352767 | None:intergenic |
| TGATACTAGTAGCAGGTGGG+TGG | 0.637051 | 2.4:+3352771 | None:intergenic |
| CTACAAAGACCGCATTGAGT+GGG | 0.639133 | 2.4:-3353026 | MS.gene85135:CDS |
| GGAGAAGAGCCTGACGTCCG+GGG | 0.642345 | 2.4:+3352576 | None:intergenic |
| CTTCTCGGCACTTCTAAGGG+AGG | 0.649267 | 2.4:-3352854 | MS.gene85135:CDS |
| CGAGACAGAGAATGAGATCG+AGG | 0.651746 | 2.4:-3352694 | MS.gene85135:CDS |
| ACTTGAGGTAGCTCGACGGA+AGG | 0.664751 | 2.4:+3353125 | None:intergenic |
| CAATGATACTAGTAGCAGGT+GGG | 0.664856 | 2.4:+3352768 | None:intergenic |
| TGCTGCTAGATCCATTGGAG+CGG | 0.676565 | 2.4:-3352960 | MS.gene85135:CDS |
| ACCCACTTGAGGTAGCTCGA+CGG | 0.680643 | 2.4:+3353121 | None:intergenic |
| CCAACAGAAGGACAACAATG+TGG | 0.706083 | 2.4:-3352654 | MS.gene85135:CDS |
| GAGCAAACTCCCACTCAATG+CGG | 0.708509 | 2.4:+3353017 | None:intergenic |
| GAGCACGCGAGTTTGAGGAG+TGG | 0.717265 | 2.4:-3353084 | MS.gene85135:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCATGTTTACTTTATGTTT+CGG | - | chr2.4:3352566-3352585 | MS.gene85135:CDS | 20.0% |
| !!! | AATAGATTTTGGAAAATACA+AGG | - | chr2.4:3352693-3352712 | MS.gene85135:CDS | 20.0% |
| !!! | ATAGATTTTGGAAAATACAA+GGG | - | chr2.4:3352694-3352713 | MS.gene85135:CDS | 20.0% |
| ! | TCATGTTTACTTTATGTTTC+GGG | - | chr2.4:3352567-3352586 | MS.gene85135:CDS | 25.0% |
| ! | TTCAGAAAGAGAAATTAAAC+TGG | - | chr2.4:3352657-3352676 | MS.gene85135:CDS | 25.0% |
| ! | TTTGCTCTTAATGTGTTAAA+TGG | - | chr2.4:3352856-3352875 | MS.gene85135:CDS | 25.0% |
| !!! | TAGATTTTGGAAAATACAAG+GGG | - | chr2.4:3352695-3352714 | MS.gene85135:CDS | 25.0% |
| AAAATCTATTACTCTGTCTC+GGG | + | chr2.4:3352683-3352702 | None:intergenic | 30.0% | |
| CAAAATCTATTACTCTGTCT+CGG | + | chr2.4:3352684-3352703 | None:intergenic | 30.0% | |
| AAAATACAAGGGGAAGATGT+TGG | - | chr2.4:3352705-3352724 | MS.gene85135:CDS | 35.0% | |
| AAATACAAGGGGAAGATGTT+GGG | - | chr2.4:3352706-3352725 | MS.gene85135:CDS | 35.0% | |
| AAATCAAAAGCAGCGTTATG+TGG | - | chr2.4:3352606-3352625 | MS.gene85135:CDS | 35.0% | |
| AATCAAAAGCAGCGTTATGT+GGG | - | chr2.4:3352607-3352626 | MS.gene85135:CDS | 35.0% | |
| ! | AAAGTTGATTTCGACCTTCT+CGG | - | chr2.4:3352988-3353007 | MS.gene85135:CDS | 35.0% |
| ! | ACTAGTATCATTGGTAGCAA+CGG | - | chr2.4:3353099-3353118 | MS.gene85135:CDS | 35.0% |
| ! | AGATAAGCAAGAGATTTGGT+TGG | - | chr2.4:3352938-3352957 | MS.gene85135:CDS | 35.0% |
| ! | ATAACCTTGACAAAGTTGGT+TGG | - | chr2.4:3352962-3352981 | MS.gene85135:CDS | 35.0% |
| ! | CGAGACAGAGTAATAGATTT+TGG | - | chr2.4:3352682-3352701 | MS.gene85135:CDS | 35.0% |
| ! | GATAAGCAAGAGATTTGGTT+GGG | - | chr2.4:3352939-3352958 | MS.gene85135:CDS | 35.0% |
| !!! | AGTTTTTTGAAACCCACTTG+AGG | + | chr2.4:3352750-3352769 | None:intergenic | 35.0% |
| ACAGTAGTAGTAGGACTAAG+AGG | - | chr2.4:3353127-3353146 | MS.gene85135:CDS | 40.0% | |
| ACGGTAGTAACAGTAGTAGT+AGG | - | chr2.4:3353118-3353137 | MS.gene85135:CDS | 40.0% | |
| CAATGATACTAGTAGCAGGT+GGG | + | chr2.4:3353092-3353111 | None:intergenic | 40.0% | |
| CAATGCGGTCTTTGTAGATT+GGG | + | chr2.4:3352828-3352847 | None:intergenic | 40.0% | |
| CAGGAGATAAGCAAGAGATT+TGG | - | chr2.4:3352934-3352953 | MS.gene85135:CDS | 40.0% | |
| CTACCAATGATACTAGTAGC+AGG | + | chr2.4:3353096-3353115 | None:intergenic | 40.0% | |
| CTTTGTAGATTGGGTCTAGA+AGG | + | chr2.4:3352819-3352838 | None:intergenic | 40.0% | |
| TAGTAGTAGGACTAAGAGGA+TGG | - | chr2.4:3353131-3353150 | MS.gene85135:CDS | 40.0% | |
| TCAATGCGGTCTTTGTAGAT+TGG | + | chr2.4:3352829-3352848 | None:intergenic | 40.0% | |
| TCGCTGAAATTGACAAGTCT+CGG | + | chr2.4:3353036-3353055 | None:intergenic | 40.0% | |
| TGGGATAACCTTGACAAAGT+TGG | - | chr2.4:3352958-3352977 | MS.gene85135:CDS | 40.0% | |
| AAATGAAGCGCACCAACAGA+AGG | - | chr2.4:3353191-3353210 | MS.gene85135:CDS | 45.0% | |
| CCAACAGAAGGACAACAATG+TGG | - | chr2.4:3353203-3353222 | MS.gene85135:CDS | 45.0% | |
| CCAATGATACTAGTAGCAGG+TGG | + | chr2.4:3353093-3353112 | None:intergenic | 45.0% | |
| CCACATTGTTGTCCTTCTGT+TGG | + | chr2.4:3353206-3353225 | None:intergenic | 45.0% | |
| CCACCTGCTACTAGTATCAT+TGG | - | chr2.4:3353090-3353109 | MS.gene85135:CDS | 45.0% | |
| CTACAAAGACCGCATTGAGT+GGG | - | chr2.4:3352831-3352850 | MS.gene85135:CDS | 45.0% | |
| TCTACAAAGACCGCATTGAG+TGG | - | chr2.4:3352830-3352849 | MS.gene85135:CDS | 45.0% | |
| TGCTCCAACCAACTTTGTCA+AGG | + | chr2.4:3352969-3352988 | None:intergenic | 45.0% | |
| !! | GTTAGGTTGGGTGTTGGTAT+TGG | + | chr2.4:3353067-3353086 | None:intergenic | 45.0% |
| CGAGACAGAGAATGAGATCG+AGG | - | chr2.4:3353163-3353182 | MS.gene85135:CDS | 50.0% | |
| GACCTTCTCGGCACTTCTAA+GGG | - | chr2.4:3353000-3353019 | MS.gene85135:CDS | 50.0% | |
| GAGCAAACTCCCACTCAATG+CGG | + | chr2.4:3352843-3352862 | None:intergenic | 50.0% | |
| TAGGATGCTGCCATGGTTGA+TGG | + | chr2.4:3353313-3353332 | None:intergenic | 50.0% | |
| TCTGCTGCTGCTAGATCCAT+TGG | - | chr2.4:3352892-3352911 | MS.gene85135:CDS | 50.0% | |
| TGATACTAGTAGCAGGTGGG+TGG | + | chr2.4:3353089-3353108 | None:intergenic | 50.0% | |
| TGCTGCTAGATCCATTGGAG+CGG | - | chr2.4:3352897-3352916 | MS.gene85135:CDS | 50.0% | |
| ! | CTTAAGAGCACGCGAGTTTG+AGG | - | chr2.4:3352768-3352787 | MS.gene85135:CDS | 50.0% |
| ! | TTCTCGGCACTTCTAAGGGA+GGG | - | chr2.4:3353004-3353023 | MS.gene85135:CDS | 50.0% |
| !! | TGCCATGGTTGATGGCCTTA+CGG | + | chr2.4:3353305-3353324 | None:intergenic | 50.0% |
| ACCCACTTGAGGTAGCTCGA+CGG | + | chr2.4:3352739-3352758 | None:intergenic | 55.0% | |
| ACGTCAGGCTCTTCTCCGTA+AGG | - | chr2.4:3353287-3353306 | MS.gene85135:CDS | 55.0% | |
| ACTTGAGGTAGCTCGACGGA+AGG | + | chr2.4:3352735-3352754 | None:intergenic | 55.0% | |
| AGCACGCGAGTTTGAGGAGT+GGG | - | chr2.4:3352774-3352793 | MS.gene85135:CDS | 55.0% | |
| AGCAGGTGGGTGGTTAGGTT+GGG | + | chr2.4:3353079-3353098 | None:intergenic | 55.0% | |
| CGACCTTCTCGGCACTTCTA+AGG | - | chr2.4:3352999-3353018 | MS.gene85135:CDS | 55.0% | |
| CTAGTAGCAGGTGGGTGGTT+AGG | + | chr2.4:3353084-3353103 | None:intergenic | 55.0% | |
| CTCAGAATCTGCCGCTCCAA+TGG | + | chr2.4:3352911-3352930 | None:intergenic | 55.0% | |
| CTCCGTAAGGCCATCAACCA+TGG | - | chr2.4:3353300-3353319 | MS.gene85135:CDS | 55.0% | |
| GCCATTGCCAATCCATTCCC+CGG | - | chr2.4:3353264-3353283 | MS.gene85135:CDS | 55.0% | |
| TAGCAGGTGGGTGGTTAGGT+TGG | + | chr2.4:3353080-3353099 | None:intergenic | 55.0% | |
| TCCGTCGAGCTACCTCAAGT+GGG | - | chr2.4:3352735-3352754 | MS.gene85135:CDS | 55.0% | |
| TTCCGTCGAGCTACCTCAAG+TGG | - | chr2.4:3352734-3352753 | MS.gene85135:CDS | 55.0% | |
| ! | CTTCTCGGCACTTCTAAGGG+AGG | - | chr2.4:3353003-3353022 | MS.gene85135:CDS | 55.0% |
| !! | CTCCCTTAGAAGTGCCGAGA+AGG | + | chr2.4:3353005-3353024 | None:intergenic | 55.0% |
| !! | TCCGGGGAATGGATTGGCAA+TGG | + | chr2.4:3353268-3353287 | None:intergenic | 55.0% |
| !! | TGGGTGGTTAGGTTGGGTGT+TGG | + | chr2.4:3353073-3353092 | None:intergenic | 55.0% |
| ACGGAGAAGAGCCTGACGTC+CGG | + | chr2.4:3353286-3353305 | None:intergenic | 60.0% | |
| AGCGGCAGATTCTGAGCTGC+AGG | - | chr2.4:3352915-3352934 | MS.gene85135:CDS | 60.0% | |
| CAATCCATTCCCCGGACGTC+AGG | - | chr2.4:3353272-3353291 | MS.gene85135:CDS | 60.0% | |
| CTGACGTCCGGGGAATGGAT+TGG | + | chr2.4:3353274-3353293 | None:intergenic | 60.0% | |
| GAGCACGCGAGTTTGAGGAG+TGG | - | chr2.4:3352773-3352792 | MS.gene85135:CDS | 60.0% | |
| AGAGCCTGACGTCCGGGGAA+TGG | + | chr2.4:3353279-3353298 | None:intergenic | 65.0% | |
| CGGAGAAGAGCCTGACGTCC+GGG | + | chr2.4:3353285-3353304 | None:intergenic | 65.0% | |
| GGAGAAGAGCCTGACGTCCG+GGG | + | chr2.4:3353284-3353303 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 3352541 | 3353338 | 3352541 | ID=MS.gene85135 |
| chr2.4 | mRNA | 3352541 | 3353338 | 3352541 | ID=MS.gene85135.t1;Parent=MS.gene85135 |
| chr2.4 | exon | 3352541 | 3353338 | 3352541 | ID=MS.gene85135.t1.exon1;Parent=MS.gene85135.t1 |
| chr2.4 | CDS | 3352541 | 3353338 | 3352541 | ID=cds.MS.gene85135.t1;Parent=MS.gene85135.t1 |
| Gene Sequence |
| Protein sequence |
