AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0744030


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0744030 MtrunA17_Chr5g0446481 56.250 112 30 3 3 95 269 380 1.33e-15 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCCCTCATCGTTGTCTTC+AGG 0.242628 5_1:+64693669 None:intergenic
TTCTTCCATGACTTCTAATT+CGG 0.254904 5_1:+64693701 None:intergenic
CTGTTCTCACATTTGACTTC+AGG 0.259207 5_1:+64693487 None:intergenic
TACCATCCAACTCCTCTTCT+CGG 0.356543 5_1:+64693449 None:intergenic
TGCCCTCATCGTTGTCTTCA+GGG 0.390187 5_1:+64693670 None:intergenic
TCTTCCATGACTTCTAATTC+GGG 0.395461 5_1:+64693702 None:intergenic
GGAGTTGGATGGTAAGGATA+AGG 0.416898 5_1:-64693440 Msa0744030:CDS
TGTTCTCACATTTGACTTCA+GGG 0.423382 5_1:+64693488 None:intergenic
AAGGAGAAAAGTCAAGAAGA+GGG 0.437816 5_1:-64693642 Msa0744030:CDS
GAAGATATTGAAGTTGAGAA+AGG 0.451322 5_1:-64693385 Msa0744030:CDS
TGTCAAAGTGGAGGAAGGAT+TGG 0.460101 5_1:-64693595 Msa0744030:CDS
GAAACCCGAATTAGAAGTCA+TGG 0.483277 5_1:-64693706 Msa0744030:CDS
GAAAAGCCGAGAAGAGGAGT+TGG 0.483883 5_1:-64693455 Msa0744030:CDS
CAACGATGAGGGCAGTGAAA+AGG 0.510264 5_1:-64693661 Msa0744030:CDS
CAAATGTGAGAACAGTGAAA+AGG 0.511793 5_1:-64693479 Msa0744030:CDS
GAGAGGAGGAAACAACCATT+AGG 0.513456 5_1:-64693347 None:intergenic
AGAAGAGGAGTTGGATGGTA+AGG 0.515883 5_1:-64693446 Msa0744030:CDS
GACGATAAGTCAAATGAAGA+TGG 0.529347 5_1:-64693415 Msa0744030:CDS
GGAGAAAAGTCAAGAAGAGG+GGG 0.533848 5_1:-64693640 Msa0744030:CDS
AGGAGAAAAGTCAAGAAGAG+GGG 0.535131 5_1:-64693641 Msa0744030:CDS
GAAGATGTCAAAGTGGAGGA+AGG 0.545672 5_1:-64693600 Msa0744030:CDS
AAAGGAGAAAAGTCAAGAAG+AGG 0.574837 5_1:-64693643 Msa0744030:CDS
TGCAGGAAAGGAAAGGTCGA+AGG 0.593802 5_1:-64693567 Msa0744030:CDS
GTCAAATGAAGATGTCAAAG+TGG 0.615078 5_1:-64693607 Msa0744030:CDS
AAAGGAGAAAAGCCGAGAAG+AGG 0.615277 5_1:-64693461 Msa0744030:CDS
ATCCCTGAAGACAACGATGA+GGG 0.622827 5_1:-64693672 Msa0744030:CDS
AAATGAAGATGTCAAAGTGG+AGG 0.626631 5_1:-64693604 Msa0744030:CDS
AGCCGAGAAGAGGAGTTGGA+TGG 0.655744 5_1:-64693451 Msa0744030:CDS
GCAGGAAAGGAAAGGTCGAA+GGG 0.655802 5_1:-64693566 Msa0744030:intron
TATCCCTGAAGACAACGATG+AGG 0.692736 5_1:-64693673 Msa0744030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAGGAATAAAAAAGTGT+GGG - chr5_1:64693723-64693742 Msa0744030:CDS 25.0%
TTCTTCCATGACTTCTAATT+CGG + chr5_1:64693394-64693413 None:intergenic 30.0%
AAGGTGAAAAAGGAAAACAA+AGG - chr5_1:64693552-64693571 Msa0744030:intron 30.0%
AAAGGAACAAAAAGAGATAG+AGG - chr5_1:64693570-64693589 Msa0744030:CDS 30.0%
GAAGATATTGAAGTTGAGAA+AGG - chr5_1:64693707-64693726 Msa0744030:CDS 30.0%
GAGAAAGGAATAAAAAAGTG+TGG - chr5_1:64693722-64693741 Msa0744030:CDS 30.0%
TCTTCCATGACTTCTAATTC+GGG + chr5_1:64693393-64693412 None:intergenic 35.0%
AAAGGAGAAAAGTCAAGAAG+AGG - chr5_1:64693449-64693468 Msa0744030:CDS 35.0%
AAGGAGAAAAGTCAAGAAGA+GGG - chr5_1:64693450-64693469 Msa0744030:CDS 35.0%
GTCAAATGAAGATGTCAAAG+TGG - chr5_1:64693485-64693504 Msa0744030:CDS 35.0%
AAATGAAGATGTCAAAGTGG+AGG - chr5_1:64693488-64693507 Msa0744030:CDS 35.0%
CAAAAAGAGATAGAGGATAG+AGG - chr5_1:64693577-64693596 Msa0744030:CDS 35.0%
! TGTTCTCACATTTGACTTCA+GGG + chr5_1:64693607-64693626 None:intergenic 35.0%
CAAATGTGAGAACAGTGAAA+AGG - chr5_1:64693613-64693632 Msa0744030:CDS 35.0%
GACGATAAGTCAAATGAAGA+TGG - chr5_1:64693677-64693696 Msa0744030:CDS 35.0%
GAAACCCGAATTAGAAGTCA+TGG - chr5_1:64693386-64693405 Msa0744030:CDS 40.0%
AGGAGAAAAGTCAAGAAGAG+GGG - chr5_1:64693451-64693470 Msa0744030:CDS 40.0%
AGGAAGGATTGGAAAAATGC+AGG - chr5_1:64693508-64693527 Msa0744030:intron 40.0%
GGATTGGAAAAATGCAGGAA+AGG - chr5_1:64693513-64693532 Msa0744030:intron 40.0%
GGAAAAATGCAGGAAAGGAA+AGG - chr5_1:64693518-64693537 Msa0744030:intron 40.0%
! CTGTTCTCACATTTGACTTC+AGG + chr5_1:64693608-64693627 None:intergenic 40.0%
! GGAATAAAAAAGTGTGGGAG+AGG - chr5_1:64693728-64693747 Msa0744030:CDS 40.0%
! ATAAAAAAGTGTGGGAGAGG+AGG - chr5_1:64693731-64693750 Msa0744030:CDS 40.0%
TATCCCTGAAGACAACGATG+AGG - chr5_1:64693419-64693438 Msa0744030:CDS 45.0%
ATCCCTGAAGACAACGATGA+GGG - chr5_1:64693420-64693439 Msa0744030:CDS 45.0%
GGAGAAAAGTCAAGAAGAGG+GGG - chr5_1:64693452-64693471 Msa0744030:CDS 45.0%
GAAGATGTCAAAGTGGAGGA+AGG - chr5_1:64693492-64693511 Msa0744030:CDS 45.0%
TGTCAAAGTGGAGGAAGGAT+TGG - chr5_1:64693497-64693516 Msa0744030:CDS 45.0%
CGAAGGGTAGAAGGTGAAAA+AGG - chr5_1:64693542-64693561 Msa0744030:intron 45.0%
AAAGGAGAAAAGCCGAGAAG+AGG - chr5_1:64693631-64693650 Msa0744030:CDS 45.0%
TACCATCCAACTCCTCTTCT+CGG + chr5_1:64693646-64693665 None:intergenic 45.0%
AGAAGAGGAGTTGGATGGTA+AGG - chr5_1:64693646-64693665 Msa0744030:CDS 45.0%
GGAGTTGGATGGTAAGGATA+AGG - chr5_1:64693652-64693671 Msa0744030:CDS 45.0%
TGCCCTCATCGTTGTCTTCA+GGG + chr5_1:64693425-64693444 None:intergenic 50.0%
CAACGATGAGGGCAGTGAAA+AGG - chr5_1:64693431-64693450 Msa0744030:CDS 50.0%
GAAAAGTCAAGAAGAGGGGG+AGG - chr5_1:64693455-64693474 Msa0744030:CDS 50.0%
TGCAGGAAAGGAAAGGTCGA+AGG - chr5_1:64693525-64693544 Msa0744030:intron 50.0%
GCAGGAAAGGAAAGGTCGAA+GGG - chr5_1:64693526-64693545 Msa0744030:intron 50.0%
AGGAAAGGTCGAAGGGTAGA+AGG - chr5_1:64693533-64693552 Msa0744030:intron 50.0%
GAAAAGCCGAGAAGAGGAGT+TGG - chr5_1:64693637-64693656 Msa0744030:CDS 50.0%
CTGCCCTCATCGTTGTCTTC+AGG + chr5_1:64693426-64693445 None:intergenic 55.0%
AGCCGAGAAGAGGAGTTGGA+TGG - chr5_1:64693641-64693660 Msa0744030:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 64693348 64693766 64693348 ID=Msa0744030;Name=Msa0744030
chr5_1 mRNA 64693348 64693766 64693348 ID=Msa0744030-mRNA-1;Parent=Msa0744030;Name=Msa0744030-mRNA-1;_AED=0.40;_eAED=0.57;_QI=0|0|0|1|0|0|2|0|124
chr5_1 exon 64693567 64693766 64693567 ID=Msa0744030-mRNA-1:exon:19182;Parent=Msa0744030-mRNA-1
chr5_1 exon 64693348 64693522 64693348 ID=Msa0744030-mRNA-1:exon:19181;Parent=Msa0744030-mRNA-1
chr5_1 CDS 64693567 64693766 64693567 ID=Msa0744030-mRNA-1:cds;Parent=Msa0744030-mRNA-1
chr5_1 CDS 64693348 64693522 64693348 ID=Msa0744030-mRNA-1:cds;Parent=Msa0744030-mRNA-1
Gene Sequence

>Msa0744030

ATGGATAGTAAAAAAGCGAGAGAAAGCGTTGAAGCTAAGAAACCCGAATTAGAAGTCATGGAAGAAGAGATTATCCCTGAAGACAACGATGAGGGCAGTGAAAAGGAGAAAAGTCAAGAAGAGGGGGAGGATGATAAGTCAAATGAAGATGTCAAAGTGGAGGAAGGATTGGAAAAATGCAGGAAAGGAAAGGTCGAAGGGATAGAGGATATCCCTGAAGTCAAATGTGAGAACAGTGAAAAGGAGAAAAGCCGAGAAGAGGAGTTGGATGGTAAGGATAAGGTTGACGATAAGTCAAATGAAGATGGTTCTTCTGAAGATATTGAAGTTGAGAAAGGAATAAAAAAGTGTGGGAGAGGAGGAAACAACCATTAG

Protein sequence

>Msa0744030

MDSKKARESVEAKKPELEVMEEEIIPEDNDEGSEKEKSQEEGEDDKSNEDVKVEEGLEKCRKGKVEGIEDIPEVKCENSEKEKSREEELDGKDKVDDKSNEDGSSEDIEVEKGIKKCGRGGNNH*