AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1124310


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1124310 MtrunA17_Chr7g0264661 94.545 55 3 0 1 55 1 55 7.81e-32 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 16 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCAGGCTGTTATCAATTA+TGG 0.292579 7_3:-75499895 Msa1124310:intron
ATGGTTGGTTACGGGAGTTT+TGG 0.322877 7_3:+75499992 None:intergenic
TCCCTTCTTCCATATCAAAA+TGG 0.350979 7_3:+75500132 None:intergenic
CAAGTTAGAGTGACGGTTGA+TGG 0.369290 7_3:+75500070 None:intergenic
TTATCAATTATGGCGCTTCA+TGG 0.376385 7_3:-75499885 None:intergenic
GAAGAAGGGAACACAAAGAA+AGG 0.486998 7_3:-75500119 Msa1124310:CDS
TCCATATCAAAATGGTGAAA+CGG 0.490271 7_3:+75500140 None:intergenic
AAGTTAGAGTGACGGTTGAT+GGG 0.529250 7_3:+75500071 None:intergenic
GAAATCATCATCGGAGGAAG+CGG 0.552988 7_3:+75500043 None:intergenic
AAGAAAGGGTTGGAAGGAAG+TGG 0.557845 7_3:-75500104 Msa1124310:CDS
CGGATTTCAAGTTAGAGTGA+CGG 0.570849 7_3:+75500063 None:intergenic
AGGGAACACAAAGAAAGGGT+TGG 0.591036 7_3:-75500114 Msa1124310:CDS
AACACAAAGAAAGGGTTGGA+AGG 0.614375 7_3:-75500110 Msa1124310:CDS
AAGAAGGGAACACAAAGAAA+GGG 0.617658 7_3:-75500118 Msa1124310:CDS
GATTTGTGTGAAATCATCAT+CGG 0.655539 7_3:+75500034 None:intergenic
TTGTGTGAAATCATCATCGG+AGG 0.661069 7_3:+75500037 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAAAAAGTGTAGTAAGAA+TGG + chr7_3:75500108-75500127 None:intergenic 20.0%
TGAAAAACACGTTCAGATAA+AGG + chr7_3:75499915-75499934 None:intergenic 30.0%
TCCATATCAAAATGGTGAAA+CGG + chr7_3:75499941-75499960 None:intergenic 30.0%
!! ACCATTTTGATATGGAAGAA+GGG - chr7_3:75499945-75499964 Msa1124310:intron 30.0%
GATTTGTGTGAAATCATCAT+CGG + chr7_3:75500047-75500066 None:intergenic 30.0%
! AAAAGTGTAGTAAGAATGGT+TGG + chr7_3:75500104-75500123 None:intergenic 30.0%
!! TCCGTTTCACCATTTTGATA+TGG - chr7_3:75499937-75499956 Msa1124310:intron 35.0%
!! CACCATTTTGATATGGAAGA+AGG - chr7_3:75499944-75499963 Msa1124310:intron 35.0%
TCCCTTCTTCCATATCAAAA+TGG + chr7_3:75499949-75499968 None:intergenic 35.0%
AAGAAGGGAACACAAAGAAA+GGG - chr7_3:75499960-75499979 Msa1124310:intron 35.0%
! TAGTAAGAATGGTTGGTTAC+GGG + chr7_3:75500097-75500116 None:intergenic 35.0%
! GTAGTAAGAATGGTTGGTTA+CGG + chr7_3:75500098-75500117 None:intergenic 35.0%
CGTGTTTGAAAATTGTTTGC+AGG - chr7_3:75500167-75500186 Msa1124310:five_prime_UTR 35.0%
TTGCAGGCTGTTATCAATTA+TGG - chr7_3:75500183-75500202 Msa1124310:five_prime_UTR 35.0%
GAAGAAGGGAACACAAAGAA+AGG - chr7_3:75499959-75499978 Msa1124310:intron 40.0%
AACACAAAGAAAGGGTTGGA+AGG - chr7_3:75499968-75499987 Msa1124310:intron 40.0%
AAGTTAGAGTGACGGTTGAT+GGG + chr7_3:75500010-75500029 None:intergenic 40.0%
!! CGGATTTCAAGTTAGAGTGA+CGG + chr7_3:75500018-75500037 None:intergenic 40.0%
TTGTGTGAAATCATCATCGG+AGG + chr7_3:75500044-75500063 None:intergenic 40.0%
AGGGAACACAAAGAAAGGGT+TGG - chr7_3:75499964-75499983 Msa1124310:intron 45.0%
AAGAAAGGGTTGGAAGGAAG+TGG - chr7_3:75499974-75499993 Msa1124310:intron 45.0%
CAAGTTAGAGTGACGGTTGA+TGG + chr7_3:75500011-75500030 None:intergenic 45.0%
GAAATCATCATCGGAGGAAG+CGG + chr7_3:75500038-75500057 None:intergenic 45.0%
!! ATGGTTGGTTACGGGAGTTT+TGG + chr7_3:75500089-75500108 None:intergenic 45.0%
!! GTTGGTTACGGGAGTTTTGG+AGG + chr7_3:75500086-75500105 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 75499888 75500212 75499888 ID=Msa1124310;Name=Msa1124310
chr7_3 mRNA 75499888 75500212 75499888 ID=Msa1124310-mRNA-1;Parent=Msa1124310;Name=Msa1124310-mRNA-1;_AED=0.00;_eAED=0.00;_QI=68|1|0.5|1|1|0.5|2|0|55
chr7_3 exon 75499888 75499911 75499888 ID=Msa1124310-mRNA-1:exon:20350;Parent=Msa1124310-mRNA-1
chr7_3 exon 75500004 75500212 75500004 ID=Msa1124310-mRNA-1:exon:20349;Parent=Msa1124310-mRNA-1
chr7_3 five_prime_UTR 75500145 75500212 75500145 ID=Msa1124310-mRNA-1:five_prime_utr;Parent=Msa1124310-mRNA-1
chr7_3 CDS 75500004 75500144 75500004 ID=Msa1124310-mRNA-1:cds;Parent=Msa1124310-mRNA-1
chr7_3 CDS 75499888 75499911 75499888 ID=Msa1124310-mRNA-1:cds;Parent=Msa1124310-mRNA-1
Gene Sequence

>Msa1124310

ATGGAAGAAGGGAACACAAAGAAAGGGTTGGAAGGAAGTGGCTTAGTTTTACCCATCAACCGTCACTCTAACTTGAAATCCGCTTCCTCCGATGATGATTTCACACAAATCATCACAAACATCAAATCCTCCAAAACTCCCGCTGTTATCAATTATGGCGCTTCA

Protein sequence

>Msa1124310

MEEGNTKKGLEGSGLVLPINRHSNLKSASSDDDFTQIITNIKSSKTPAVINYGAS