AlfalfaGEDB | Msa0816640

M. sativa cultivar zhongmu-4 / Msa0816640

KEGG: K06941
GO: -
PFAM: PF04055.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0816640	sp\|B0C9F4\|RLMN_ACAM1	45.858	338	133	5	43	339	13	341	5.87e-100	303

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0816640	A0A396HUE3	86.585	410	13	2	1	368	1	410	0.0	713

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0020630	Msa0816640	0.834469	2.944558e-56	-8.615850e-47
Msa0027540	Msa0816640	0.830285	3.200349e-55	-8.615850e-47
Msa0030380	Msa0816640	0.816625	5.021336e-52	-8.615850e-47
Msa0038350	Msa0816640	-0.809485	1.848347e-50	-8.615850e-47
Msa0044710	Msa0816640	0.877492	6.015780e-69	-8.615850e-47
Msa0050720	Msa0816640	0.836486	9.098047e-57	-8.615850e-47
Msa0050770	Msa0816640	0.850656	1.474886e-60	-8.615850e-47
Msa0056520	Msa0816640	0.814233	1.710241e-51	-8.615850e-47
Msa0056690	Msa0816640	0.813803	2.127277e-51	-8.615850e-47
Msa0075310	Msa0816640	0.838914	2.166013e-57	-8.615850e-47
Msa0084680	Msa0816640	0.817308	3.526662e-52	-8.615850e-47
Msa0091010	Msa0816640	0.816952	4.240484e-52	-8.615850e-47
Msa0091770	Msa0816640	0.811326	7.405565e-51	-8.615850e-47
Msa0605170	Msa0816640	0.817716	2.853935e-52	-8.615850e-47
Msa0607340	Msa0816640	-0.805854	1.091563e-49	-8.615850e-47
Msa0618010	Msa0816640	0.807699	4.449412e-50	-8.615850e-47
Msa0621120	Msa0816640	0.814999	1.157384e-51	-8.615850e-47
Msa0628100	Msa0816640	0.819519	1.113297e-52	-8.615850e-47
Msa0630990	Msa0816640	0.802541	5.341831e-49	-8.615850e-47
Msa0638280	Msa0816640	0.811636	6.340090e-51	-8.615850e-47
Msa0638320	Msa0816640	0.811427	7.039367e-51	-8.615850e-47
Msa0647450	Msa0816640	-0.834355	3.144961e-56	-8.615850e-47
Msa0657050	Msa0816640	0.806202	9.225029e-50	-8.615850e-47
Msa0661240	Msa0816640	0.814619	1.404791e-51	-8.615850e-47
Msa0672120	Msa0816640	0.809295	2.030503e-50	-8.615850e-47
Msa0677270	Msa0816640	0.805694	1.179379e-49	-8.615850e-47
Msa0684510	Msa0816640	0.813453	2.539693e-51	-8.615850e-47
Msa0701030	Msa0816640	0.808065	3.718604e-50	-8.615850e-47
Msa0704220	Msa0816640	0.802567	5.275998e-49	-8.615850e-47
Msa0714600	Msa0816640	0.834448	2.979781e-56	-8.615850e-47
Msa0728280	Msa0816640	0.821218	4.538856e-53	-8.615850e-47
Msa0728380	Msa0816640	0.817989	2.477408e-52	-8.615850e-47
Msa0728540	Msa0816640	0.822591	2.182588e-53	-8.615850e-47
Msa0734660	Msa0816640	0.809299	2.026842e-50	-8.615850e-47
Msa0737440	Msa0816640	0.823977	1.035820e-53	-8.615850e-47
Msa0743650	Msa0816640	0.821125	4.766877e-53	-8.615850e-47
Msa0776220	Msa0816640	0.808361	3.217197e-50	-8.615850e-47
Msa0776480	Msa0816640	0.828287	9.776540e-55	-8.615850e-47
Msa0779240	Msa0816640	0.904107	1.733877e-79	-8.615850e-47
Msa0795920	Msa0816640	0.815680	8.165310e-52	-8.615850e-47
Msa0813670	Msa0816640	0.820562	6.423049e-53	-8.615850e-47
Msa0813720	Msa0816640	0.811620	6.392738e-51	-8.615850e-47
Msa0816640	Msa0828570	0.812059	5.127943e-51	-8.615850e-47
Msa0816640	Msa0842210	0.804321	2.285104e-49	-8.615850e-47
Msa0816640	Msa0863770	0.824110	9.637639e-54	-8.615850e-47
Msa0816640	Msa0909710	0.822797	1.954254e-53	-8.615850e-47
Msa0816640	Msa0950320	0.826639	2.429733e-54	-8.615850e-47
Msa0816640	Msa1007610	-0.812980	3.225631e-51	-8.615850e-47
Msa0816640	Msa1018020	-0.806384	8.446348e-50	-8.615850e-47
Msa0816640	Msa1038320	0.803035	4.223594e-49	-8.615850e-47
Msa0816640	Msa1046610	0.820673	6.056822e-53	-8.615850e-47
Msa0816640	Msa1051200	0.800416	1.456190e-48	-8.615850e-47
Msa0816640	Msa1072460	-0.829754	4.313783e-55	-8.615850e-47
Msa0816640	Msa1103650	0.818059	2.388087e-52	-8.615850e-47
Msa0816640	Msa1111630	0.843860	1.080868e-58	-8.615850e-47
Msa0816640	Msa1112000	0.807933	3.967319e-50	-8.615850e-47
Msa0816640	Msa1132890	0.802426	5.642433e-49	-8.615850e-47
Msa0816640	Msa1167170	0.830812	2.378596e-55	-8.615850e-47
Msa0816640	Msa1171690	0.802100	6.584570e-49	-8.615850e-47
Msa0816640	Msa1177240	0.838313	3.096520e-57	-8.615850e-47
Msa0816640	Msa1187520	0.805651	1.204251e-49	-8.615850e-47
Msa0816640	Msa1188940	0.831562	1.556103e-55	-8.615850e-47
Msa0816640	Msa1204980	-0.800170	1.634382e-48	-8.615850e-47
Msa0816640	Msa1205010	-0.805606	1.230760e-49	-8.615850e-47
Msa0816640	Msa1217860	0.812526	4.056752e-51	-8.615850e-47
Msa0816640	Msa1256540	-0.806237	9.067037e-50	-8.615850e-47
Msa0816640	Msa1261470	0.800958	1.128678e-48	-8.615850e-47
Msa0816640	Msa1265960	0.822760	1.994086e-53	-8.615850e-47
Msa0816640	Msa1282920	0.801753	7.759231e-49	-8.615850e-47
Msa0816640	Msa1284520	0.821193	4.599342e-53	-8.615850e-47
Msa0816640	Msa1296960	-0.808288	3.333010e-50	-8.615850e-47
Msa0816640	Msa1304180	0.803114	4.067680e-49	-8.615850e-47
Msa0816640	Msa1317030	0.863076	3.222856e-64	-8.615850e-47
Msa0816640	Msa1322190	0.800835	1.196135e-48	-8.615850e-47
Msa0816640	Msa1324000	0.818050	2.399006e-52	-8.615850e-47
Msa0816640	Msa1335970	-0.809139	2.193261e-50	-8.615850e-47
Msa0816640	Msa1347290	0.819936	8.936093e-53	-8.615850e-47
Msa0816640	Msa1347620	0.810609	1.058739e-50	-8.615850e-47
Msa0816640	Msa1375340	0.800161	1.641147e-48	-8.615850e-47
Msa0816640	Msa1410290	0.807018	6.203948e-50	-8.615850e-47
Msa0816640	Msa1421870	0.810521	1.106003e-50	-8.615850e-47
Msa0816640	Msa1427830	0.850616	1.513499e-60	-8.615850e-47
Msa0816640	Msa1431000	0.807356	5.262249e-50	-8.615850e-47
Msa0816640	Msa1439960	0.804951	1.688107e-49	-8.615850e-47
Msa0816640	Msa1440610	0.837264	5.759011e-57	-8.615850e-47
Msa0816640	Msa1443290	-0.800192	1.617114e-48	-8.615850e-47
Msa0816640	Msa1447300	0.811217	7.818136e-51	-8.615850e-47
Msa0816640	Msa1449880	0.824298	8.706113e-54	-8.615850e-47
Msa0816640	Msa1453620	0.820391	7.030774e-53	-8.615850e-47
Msa0816640	Msa1454110	0.832318	1.012482e-55	-8.615850e-47
Msa0816640	Msa1460440	0.812074	5.090866e-51	-8.615850e-47
Msa0159750	Msa0816640	0.832204	1.080212e-55	-8.615850e-47
Msa0168100	Msa0816640	0.838845	2.257305e-57	-8.615850e-47
Msa0173740	Msa0816640	0.822743	2.011846e-53	-8.615850e-47
Msa0173820	Msa0816640	0.816378	5.703945e-52	-8.615850e-47
Msa0173830	Msa0816640	0.830460	2.900696e-55	-8.615850e-47
Msa0182930	Msa0816640	0.845062	5.132532e-59	-8.615850e-47
Msa0183370	Msa0816640	0.803409	3.534474e-49	-8.615850e-47
Msa0185640	Msa0816640	-0.811959	5.393977e-51	-8.615850e-47
Msa0200950	Msa0816640	-0.832637	8.435337e-56	-8.615850e-47
Msa0376510	Msa0816640	0.814019	1.906442e-51	-8.615850e-47
Msa0378080	Msa0816640	0.807437	5.057585e-50	-8.615850e-47
Msa0390060	Msa0816640	0.805767	1.138451e-49	-8.615850e-47
Msa0391630	Msa0816640	0.823240	1.540450e-53	-8.615850e-47
Msa0418430	Msa0816640	0.814875	1.232690e-51	-8.615850e-47
Msa0425050	Msa0816640	0.817580	3.063836e-52	-8.615850e-47
Msa0426160	Msa0816640	0.800875	1.173696e-48	-8.615850e-47
Msa0443500	Msa0816640	0.817201	3.729428e-52	-8.615850e-47
Msa0448080	Msa0816640	0.800331	1.515190e-48	-8.615850e-47
Msa0462590	Msa0816640	0.814955	1.183752e-51	-8.615850e-47
Msa0465790	Msa0816640	0.815928	7.188620e-52	-8.615850e-47
Msa0468160	Msa0816640	0.812798	3.536886e-51	-8.615850e-47
Msa0470180	Msa0816640	0.815055	1.124333e-51	-8.615850e-47
Msa0472740	Msa0816640	0.807827	4.178340e-50	-8.615850e-47
Msa0482680	Msa0816640	0.806813	6.854420e-50	-8.615850e-47
Msa0244270	Msa0816640	0.803134	4.030000e-49	-8.615850e-47
Msa0252180	Msa0816640	0.806153	9.444516e-50	-8.615850e-47
Msa0258670	Msa0816640	-0.850895	1.263203e-60	-8.615850e-47
Msa0265580	Msa0816640	0.808101	3.654542e-50	-8.615850e-47
Msa0267310	Msa0816640	0.819824	9.481243e-53	-8.615850e-47
Msa0288240	Msa0816640	0.838612	2.593195e-57	-8.615850e-47
Msa0288330	Msa0816640	0.862867	3.739602e-64	-8.615850e-47
Msa0296160	Msa0816640	0.805586	1.242806e-49	-8.615850e-47
Msa0298940	Msa0816640	0.811988	5.315444e-51	-8.615850e-47
Msa0298970	Msa0816640	0.819151	1.349782e-52	-8.615850e-47
Msa0326670	Msa0816640	0.858884	6.062792e-63	-8.615850e-47
Msa0334570	Msa0816640	0.809824	1.562970e-50	-8.615850e-47
Msa0342340	Msa0816640	-0.803476	3.423924e-49	-8.615850e-47
Msa0349060	Msa0816640	0.814054	1.873298e-51	-8.615850e-47
Msa0358170	Msa0816640	0.861840	7.733416e-64	-8.615850e-47
Msa0511720	Msa0816640	-0.803563	3.284729e-49	-8.615850e-47
Msa0516450	Msa0816640	0.803603	3.222469e-49	-8.615850e-47
Msa0518040	Msa0816640	0.813518	2.457515e-51	-8.615850e-47
Msa0524890	Msa0816640	-0.802371	5.792088e-49	-8.615850e-47
Msa0526640	Msa0816640	-0.808432	3.106029e-50	-8.615850e-47
Msa0530950	Msa0816640	0.832979	6.937055e-56	-8.615850e-47
Msa0532300	Msa0816640	0.806413	8.325381e-50	-8.615850e-47
Msa0535030	Msa0816640	0.815961	7.070173e-52	-8.615850e-47
Msa0535060	Msa0816640	0.819470	1.141834e-52	-8.615850e-47
Msa0540070	Msa0816640	0.832632	8.459657e-56	-8.615850e-47
Msa0553220	Msa0816640	-0.810922	9.059766e-51	-8.615850e-47
Msa0564930	Msa0816640	0.803631	3.179549e-49	-8.615850e-47
Msa0571610	Msa0816640	0.835102	2.040128e-56	-8.615850e-47
Msa0580420	Msa0816640	0.823564	1.294127e-53	-8.615850e-47
Msa0583740	Msa0816640	0.839255	1.767882e-57	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0816640	MtrunA17_Chr5g0435221	86.585	410	13	2	1	368	1	410	0.0	713
Msa0816640	MtrunA17_Chr5g0393761	34.615	286	130	7	116	348	86	367	4.13e-39	143
Msa0816640	MtrunA17_Chr8g0365351	31.781	365	174	14	40	338	81	436	1.00e-34	132

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0816640	AT2G39670.1	62.274	387	94	5	16	358	42	420	6.24e-162	461
Msa0816640	AT2G39670.2	61.538	390	95	6	16	358	42	423	2.19e-158	452
Msa0816640	AT1G60230.1	32.961	358	185	9	29	338	83	433	2.61e-44	159
Msa0816640	AT3G19630.1	34.483	290	129	9	114	346	74	359	9.70e-41	147

Find 129 sgRNAs with CRISPR-Local

Find 282 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTGACTAATGTAGTCTTTA+TGG	0.145319	5_3:+51151897	Msa0816640:CDS
AACTGAGATTAAACTCATTT+TGG	0.168528	5_3:-51153477	None:intergenic
CACCTCTATCACTTCATAAT+TGG	0.231715	5_3:-51153501	None:intergenic
GAGCCTTCTATTGGATTAAA+AGG	0.231828	5_3:-51153025	None:intergenic
TTATCAACATTCTACTTATA+TGG	0.235386	5_3:+51153408	Msa0816640:three_prime_UTR
TAGAAAGCCTTTAGATTCTT+TGG	0.238927	5_3:+51153635	Msa0816640:three_prime_UTR
TTGCAGTGCCTCAGGCATTT+AGG	0.286812	5_3:+51150724	Msa0816640:intron
GTTAAAACACAGGCGTAGTT+TGG	0.293499	5_3:+51153901	Msa0816640:three_prime_UTR
AACATGGACCTTGCAGTTAC+AGG	0.297851	5_3:+51153346	Msa0816640:CDS
GGCACACCATTGCTTGAATA+AGG	0.298869	5_3:+51151960	Msa0816640:CDS
GAGGAAGGTCAAAGATATTC+AGG	0.299020	5_3:+51150274	Msa0816640:CDS
ATTGTTGGCAATAAGTGATT+AGG	0.320504	5_3:+51153721	Msa0816640:three_prime_UTR
TGGCATATATTAAACAAGTT+TGG	0.324362	5_3:+51153428	Msa0816640:three_prime_UTR
CAAAACTCAGAGCCTTCTAT+TGG	0.327284	5_3:-51153034	None:intergenic
GTGCCCCAAGTACTGCCTTC+AGG	0.344031	5_3:-51151942	None:intergenic
GTGCATAGTGAACCACTTAA+AGG	0.352529	5_3:-51153933	None:intergenic
GAACCCTTCTCATCTTCATT+AGG	0.357570	5_3:-51151363	None:intergenic
CACACACAATGTCAGTTTCA+TGG	0.357900	5_3:+51153376	Msa0816640:three_prime_UTR
GCAAGCATTTGTTAATGCTT+TGG	0.362056	5_3:+51153186	Msa0816640:CDS
GCTAAACTACTACATGAATA+TGG	0.366452	5_3:+51152977	Msa0816640:CDS
TGCTTGCATTGCAGTGCCTC+AGG	0.385823	5_3:+51150716	Msa0816640:intron
ACATAAATATATAATGCAAA+AGG	0.390056	5_3:-51153795	None:intergenic
CCTCAGTCTCAGGTTCTCCT+TGG	0.396335	5_3:+51150044	Msa0816640:CDS
GTGACTAATGTAGTCTTTAT+GGG	0.396455	5_3:+51151898	Msa0816640:CDS
TTTGCAGTTGTTATTGAAGT+TGG	0.398916	5_3:+51151308	Msa0816640:intron
CAATTTCCAGAAAACACCTT+TGG	0.405431	5_3:+51153285	Msa0816640:CDS
AAACCTGCTCAACAATTTCA+TGG	0.407549	5_3:-51151614	None:intergenic
TATCATACTGCTTTCCTGTA+AGG	0.409585	5_3:-51153061	None:intergenic
TGCATTTACGTCCAGTCCTC+TGG	0.409881	5_3:-51153242	None:intergenic
TTACGTCCAGTCCTCTGGTT+TGG	0.413198	5_3:-51153237	None:intergenic
TTAGGACTTGGTAGTTCATA+TGG	0.417772	5_3:+51153739	Msa0816640:three_prime_UTR
CGCAGACTCTGACAACAATC+TGG	0.425413	5_3:+51153309	Msa0816640:CDS
AGGAATGAGCTTGAGGAAGC+TGG	0.428323	5_3:+51150744	Msa0816640:CDS
GCAACAACAGTGTTGAAAAC+TGG	0.434797	5_3:-51150786	None:intergenic
GTAACTGCAAGGTCCATGTT+TGG	0.434846	5_3:-51153343	None:intergenic
TTAAAACACAGGCGTAGTTT+GGG	0.435412	5_3:+51153902	Msa0816640:three_prime_UTR
ATGCTCCTAACCAGAAACTA+AGG	0.440088	5_3:+51152702	Msa0816640:intron
TTAAACCTGAAGGCAGTACT+TGG	0.440485	5_3:+51151937	Msa0816640:CDS
AAGGTCCATGTTTGGAAGCT+GGG	0.440750	5_3:-51153335	None:intergenic
CATAAATATATAATGCAAAA+GGG	0.443153	5_3:-51153794	None:intergenic
AACACTGTTGTTGCAACGGA+TGG	0.445377	5_3:+51150795	Msa0816640:CDS
CAAGGTCCATGTTTGGAAGC+TGG	0.445501	5_3:-51153336	None:intergenic
GAGCCATGAAATTGTTGAGC+AGG	0.455181	5_3:+51151611	Msa0816640:CDS
GTGCGAGGGGCATGGAATAG+TGG	0.456188	5_3:-51149950	None:intergenic
ACTTGCCCTTGACTTTGGTC+AGG	0.457092	5_3:+51150091	Msa0816640:CDS
CTACCACGAGCAAATATTGT+TGG	0.458499	5_3:+51153706	Msa0816640:three_prime_UTR
ATTGTAACTCAGGCAGCAAC+AGG	0.461549	5_3:-51153527	None:intergenic
AATGTAGTCTTTATGGGAAT+GGG	0.461630	5_3:+51151904	Msa0816640:CDS
TCGCACAATTCCTCAGTCTC+AGG	0.472816	5_3:+51150034	Msa0816640:CDS
GGCAATAAGTGATTAGGACT+TGG	0.481807	5_3:+51153727	Msa0816640:three_prime_UTR
TTTCATAAGAAACAACCTAC+TGG	0.488171	5_3:+51153672	Msa0816640:three_prime_UTR
GGTAGTTCATATGGTGCACT+TGG	0.491177	5_3:+51153748	Msa0816640:three_prime_UTR
TGCAACGGATGGAACTGTTA+AGG	0.495578	5_3:+51150806	Msa0816640:CDS
TAGATTCTTTGGCAGCTAAG+AGG	0.497835	5_3:+51153646	Msa0816640:three_prime_UTR
AGAAGAAGTTGCGAAGGAAA+TGG	0.498648	5_3:-51150006	None:intergenic
CATTCACCTTATTCAAGCAA+TGG	0.504951	5_3:-51151966	None:intergenic
TAATGTAGTCTTTATGGGAA+TGG	0.509539	5_3:+51151903	Msa0816640:CDS
TGGGATGCACATCCTTTAAG+TGG	0.510333	5_3:+51153921	Msa0816640:three_prime_UTR
CACCGCACGTGCGAGGGGCA+TGG	0.514603	5_3:-51149958	None:intergenic
TACCAATTATGAAGTGATAG+AGG	0.515017	5_3:+51153499	Msa0816640:three_prime_UTR
GCGTTTGAACACCTCCTCAA+TGG	0.524945	5_3:-51151872	None:intergenic
GACAGCATGTGTCTCATCAC+AGG	0.527434	5_3:+51151395	Msa0816640:CDS
AGATATTCAGGAGTTTACTC+AGG	0.528880	5_3:+51150286	Msa0816640:CDS
TAAACCTGAAGGCAGTACTT+GGG	0.530652	5_3:+51151938	Msa0816640:CDS
GGAATCCCAGCTTCCAAACA+TGG	0.536229	5_3:+51153330	Msa0816640:CDS
GAAAGTTGACATTGTAACTC+AGG	0.538959	5_3:-51153537	None:intergenic
TTTATCATCTTATATACAAG+AGG	0.550624	5_3:+51150255	Msa0816640:CDS
CTTGCATAAGTGCATCCAAT+GGG	0.551233	5_3:-51152754	None:intergenic
AAGTGCAAAGTCTTACCCAT+TGG	0.552672	5_3:+51152739	Msa0816640:CDS
TTAGCTGCCAAAGAATCTAA+AGG	0.556580	5_3:-51153642	None:intergenic
TGCACTTATGCAAGACTGCA+AGG	0.557442	5_3:+51152763	Msa0816640:CDS
AGGAGAAGAAGAAGTTGCGA+AGG	0.559114	5_3:-51150012	None:intergenic
ATGCAGCTTGTGGTCAGCTA+CGG	0.561024	5_3:+51153260	Msa0816640:CDS
ACCTAAAAGTGCATATTCAA+AGG	0.561121	5_3:-51152816	None:intergenic
TTTCAACACTGTTGTTGCAA+CGG	0.563769	5_3:+51150791	Msa0816640:CDS
GGCATTTAGGAATGAGCTTG+AGG	0.565772	5_3:+51150737	Msa0816640:CDS
TCTGGTTTGGCGTACACTAG+TGG	0.566254	5_3:-51153224	None:intergenic
CTTCCGCGAAACAAGTAGAC+GGG	0.568795	5_3:+51152790	Msa0816640:CDS
TATTCCTAATGAAGATGAGA+AGG	0.569257	5_3:+51151359	Msa0816640:CDS
AACAGATTGATTGAAACTGT+TGG	0.569401	5_3:+51151336	Msa0816640:CDS
TTGTCAGAGTCTGCGGCCAA+AGG	0.571073	5_3:-51153301	None:intergenic
AAGGACGCACCGCACGTGCG+AGG	0.571527	5_3:-51149965	None:intergenic
ATGAGCTTGAGGAAGCTGGA+TGG	0.572364	5_3:+51150748	Msa0816640:CDS
TTCCATGCCCCTCGCACGTG+CGG	0.573881	5_3:+51149956	Msa0816640:CDS
AATGGGTAAGACTTTGCACT+TGG	0.575944	5_3:-51152737	None:intergenic
AAACCCGTCTACTTGTTTCG+CGG	0.576482	5_3:-51152793	None:intergenic
CCAAGGAGAACCTGAGACTG+AGG	0.576686	5_3:-51150044	None:intergenic
GTGATGAGACACATGCTGTC+AGG	0.578912	5_3:-51151392	None:intergenic
GCGGCACGTGTGTGAGGTGA+AGG	0.580205	5_3:-51149984	None:intergenic
GTAGTAACTAAATTTCCAGT+AGG	0.581507	5_3:-51153687	None:intergenic
CAGATTGTTGTCAGAGTCTG+CGG	0.584126	5_3:-51153308	None:intergenic
TGTACGCCAAACCAGAGGAC+TGG	0.586064	5_3:+51153231	Msa0816640:CDS
TTGAGGAGGTGTTCAAACGC+AGG	0.587360	5_3:+51151875	Msa0816640:CDS
GGACGCACCGCACGTGCGAG+GGG	0.588242	5_3:-51149963	None:intergenic
GTTTGGACTAAAACAATGAG+AGG	0.588656	5_3:+51153445	Msa0816640:three_prime_UTR
TGAACAGGTCTTGGCCATTG+AGG	0.590271	5_3:+51151858	Msa0816640:intron
TGCTCCTAACCAGAAACTAA+GGG	0.592577	5_3:+51152703	Msa0816640:intron
AGGACGCACCGCACGTGCGA+GGG	0.593033	5_3:-51149964	None:intergenic
CTACTACATGAATATGGCAG+TGG	0.597183	5_3:+51152983	Msa0816640:CDS
AAGCTGGATGGAAGGTCGGA+CGG	0.599050	5_3:+51150760	Msa0816640:CDS
TCATCTTATATACAAGAGGA+AGG	0.599803	5_3:+51150259	Msa0816640:CDS
TGAACCAATGTTAAACCTGA+AGG	0.600380	5_3:+51151927	Msa0816640:CDS
GAGGAAGCTGGATGGAAGGT+CGG	0.601140	5_3:+51150756	Msa0816640:CDS
AATTTACCTGACCAAAGTCA+AGG	0.603496	5_3:-51150097	None:intergenic
GCTTGAGGAAGCTGGATGGA+AGG	0.604593	5_3:+51150752	Msa0816640:CDS
ACTGCCTTCAGGTTTAACAT+TGG	0.604771	5_3:-51151931	None:intergenic
GTTCGGTTTCGGATAAGCCA+AGG	0.607702	5_3:-51150061	None:intergenic
GTAGTTCATATGGTGCACTT+GGG	0.610199	5_3:+51153749	Msa0816640:three_prime_UTR
AAACCTGAAGGCAGTACTTG+GGG	0.612474	5_3:+51151939	Msa0816640:CDS
GATATTCAGGAGTTTACTCA+GGG	0.617312	5_3:+51150287	Msa0816640:CDS
TGAGGAGGTGTTCAAACGCA+GGG	0.618525	5_3:+51151876	Msa0816640:CDS
ATAAATATATAATGCAAAAG+GGG	0.620266	5_3:-51153793	None:intergenic
ATTTACCTGACCAAAGTCAA+GGG	0.630175	5_3:-51150096	None:intergenic
AGCAGCTGGAATCAATGACT+CGG	0.632061	5_3:+51152934	Msa0816640:intron
TTGCCAACAATATTTGCTCG+TGG	0.633279	5_3:-51153709	None:intergenic
TCTTGCATAAGTGCATCCAA+TGG	0.635870	5_3:-51152755	None:intergenic
ATACTGCTTTCCTGTAAGGA+CGG	0.636058	5_3:-51153057	None:intergenic
ACAGGTCTTGGCCATTGAGG+AGG	0.637192	5_3:+51151861	Msa0816640:intron
ATTCCTAATGAAGATGAGAA+GGG	0.637233	5_3:+51151360	Msa0816640:CDS
TTCTTGCTAGCAGAGAAATG+TGG	0.644446	5_3:+51153557	Msa0816640:three_prime_UTR
AGCTCATTCCTAAATGCCTG+AGG	0.647100	5_3:-51150732	None:intergenic
GCAATCAACCTGTAACTGCA+AGG	0.653477	5_3:-51153354	None:intergenic
GAAATGGCGGCACGTGTGTG+AGG	0.656275	5_3:-51149990	None:intergenic
ACTTCCGCGAAACAAGTAGA+CGG	0.656727	5_3:+51152789	Msa0816640:CDS
AATGTGGAGAAGCATCTATG+TGG	0.673433	5_3:+51153573	Msa0816640:three_prime_UTR
AGAAGTTGCGAAGGAAATGG+CGG	0.675289	5_3:-51150003	None:intergenic
TGAGACTGAGGAATTGTGCG+AGG	0.678499	5_3:-51150032	None:intergenic
ACTAGTGTACGCCAAACCAG+AGG	0.706693	5_3:+51153226	Msa0816640:CDS
CTGGACGTAAATGCAGCTTG+TGG	0.711383	5_3:+51153250	Msa0816640:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACAATAAAATGAAATATAAA+AGG	-	chr5_3:51152268-51152287	None:intergenic	10.0%
!!	AAAAAACAATAAATTAACAA+AGG	-	chr5_3:51153875-51153894	None:intergenic	10.0%
!!	ATTGAACAAATTAATAATGA+AGG	-	chr5_3:51150146-51150165	None:intergenic	15.0%
!!	TCATTGTATTAAGTTATTAT+TGG	+	chr5_3:51150442-51150461	Msa0816640:intron	15.0%
!!	AAATAAAAATGATAACAGAA+AGG	+	chr5_3:51151728-51151747	Msa0816640:intron	15.0%
!!	TAAACACGATTTATAATTTA+TGG	+	chr5_3:51153107-51153126	Msa0816640:intron	15.0%
!!	ATAAATATATAATGCAAAAG+GGG	-	chr5_3:51153796-51153815	None:intergenic	15.0%
!!	CATAAATATATAATGCAAAA+GGG	-	chr5_3:51153797-51153816	None:intergenic	15.0%
!!	ACATAAATATATAATGCAAA+AGG	-	chr5_3:51153798-51153817	None:intergenic	15.0%
!!!	GAAAAAAAAATCTGTTTTAA+CGG	-	chr5_3:51153853-51153872	None:intergenic	15.0%
!!	ACAAATTAATAATGAAGGAA+GGG	-	chr5_3:51150141-51150160	None:intergenic	20.0%
!!	AACAAATTAATAATGAAGGA+AGG	-	chr5_3:51150142-51150161	None:intergenic	20.0%
!!	TTTATCATCTTATATACAAG+AGG	+	chr5_3:51150255-51150274	Msa0816640:CDS	20.0%
!!	ATATGTATTATTGAGTAAGA+AGG	+	chr5_3:51150356-51150375	Msa0816640:intron	20.0%
!!!	TCTATTTTATCAACTATTCA+TGG	+	chr5_3:51150612-51150631	Msa0816640:intron	20.0%
!!!	TTGTTGTTGTTTTTAATTTC+TGG	+	chr5_3:51150866-51150885	Msa0816640:intron	20.0%
!!!	GTAGCTTTTAGTTTTTTTAA+AGG	+	chr5_3:51151037-51151056	Msa0816640:intron	20.0%
!!!	ATTCTTGTTTTTCTATTTGT+TGG	+	chr5_3:51151277-51151296	Msa0816640:intron	20.0%
!!!	TTACAAGCAAATATTTGTTA+AGG	-	chr5_3:51152018-51152037	None:intergenic	20.0%
!!	AATTATTACTGTTCTCTATA+TGG	+	chr5_3:51152036-51152055	Msa0816640:intron	20.0%
!!	TTAATCCAAACTTTGTAATT+TGG	+	chr5_3:51153158-51153177	Msa0816640:intron	20.0%
!!	TTATCAACATTCTACTTATA+TGG	+	chr5_3:51153408-51153427	Msa0816640:three_prime_UTR	20.0%
!!	TGATTGAATTTTTCTTAGCA+AGG	+	chr5_3:51150215-51150234	Msa0816640:intron	25.0%
!!	ATTTTTCTTAGCAAGGTTTT+AGG	+	chr5_3:51150222-51150241	Msa0816640:intron	25.0%
!!	TTTTTCTTAGCAAGGTTTTA+GGG	+	chr5_3:51150223-51150242	Msa0816640:intron	25.0%
!	TCAACAAACAAGACAAAATA+AGG	-	chr5_3:51150333-51150352	None:intergenic	25.0%
!!	ATTAAGTTATTATTGGTGTC+AGG	+	chr5_3:51150449-51150468	Msa0816640:intron	25.0%
!!!	AAAGTATTAACTTTTCGATG+TGG	+	chr5_3:51150679-51150698	Msa0816640:intron	25.0%
!	AAAACAACAACAACAACAAT+AGG	-	chr5_3:51150859-51150878	None:intergenic	25.0%
!!	TTTTTAAACTTATGTGACAC+TGG	+	chr5_3:51150980-51150999	Msa0816640:intron	25.0%
!	AAAAATGAGATCAATGTCAA+TGG	-	chr5_3:51151135-51151154	None:intergenic	25.0%
!!!	TGATTCAAGTTGTTTTCTTT+TGG	+	chr5_3:51151162-51151181	Msa0816640:intron	25.0%
!!!	TTTTCTTTTGGTGTTTTACT+TGG	+	chr5_3:51151174-51151193	Msa0816640:intron	25.0%
!	ACTTGGTATTCTTAAGAATA+AGG	+	chr5_3:51151191-51151210	Msa0816640:intron	25.0%
!	CTTGGTATTCTTAAGAATAA+GGG	+	chr5_3:51151192-51151211	Msa0816640:intron	25.0%
!!!	GATATTAACTTGCTTGTTAA+AGG	+	chr5_3:51151218-51151237	Msa0816640:intron	25.0%
!	ATCAGTGCATAAAAAAATCA+AGG	-	chr5_3:51151259-51151278	None:intergenic	25.0%
!	ATGGACATAACAAAAACAAA+TGG	-	chr5_3:51151507-51151526	None:intergenic	25.0%
!	CTAAATATCCAAATAACAGA+TGG	-	chr5_3:51151526-51151545	None:intergenic	25.0%
!!	CATCTGTTATTTGGATATTT+AGG	+	chr5_3:51151524-51151543	Msa0816640:intron	25.0%
!!	TGTTATTTGGATATTTAGGT+AGG	+	chr5_3:51151528-51151547	Msa0816640:intron	25.0%
!	AAACTACTCTAATGTATCTA+TGG	-	chr5_3:51151644-51151663	None:intergenic	25.0%
!	AATTTATTTATGGATCACGT+GGG	-	chr5_3:51151679-51151698	None:intergenic	25.0%
!	AATTGAATCTAAATTGCCTT+GGG	-	chr5_3:51151703-51151722	None:intergenic	25.0%
!	TAATTGAATCTAAATTGCCT+TGG	-	chr5_3:51151704-51151723	None:intergenic	25.0%
!	AAAATGATAACAGAAAGGAA+AGG	+	chr5_3:51151733-51151752	Msa0816640:intron	25.0%
!!	TAAAGCAATGAGATCATTTT+CGG	+	chr5_3:51151768-51151787	Msa0816640:intron	25.0%
!!	ACATTGAATTTTATGTGAAC+AGG	+	chr5_3:51151843-51151862	Msa0816640:intron	25.0%
!!	CGATAAATGATTTCTTTTGA+CGG	-	chr5_3:51152066-51152085	None:intergenic	25.0%
!	CGTCAAAAGAAATCATTTAT+CGG	+	chr5_3:51152064-51152083	Msa0816640:intron	25.0%
!	GCTAACTTCTTTATTGTATT+TGG	-	chr5_3:51152408-51152427	None:intergenic	25.0%
!!	CCATCAAATGATATATTTTG+TGG	-	chr5_3:51152594-51152613	None:intergenic	25.0%
!	CCACAAAATATATCATTTGA+TGG	+	chr5_3:51152591-51152610	Msa0816640:intron	25.0%
!!	ATACCTTTTAATCCAATAGA+AGG	+	chr5_3:51153022-51153041	Msa0816640:CDS	25.0%
!	TGGCATATATTAAACAAGTT+TGG	+	chr5_3:51153428-51153447	Msa0816640:three_prime_UTR	25.0%
!!	AACTGAGATTAAACTCATTT+TGG	-	chr5_3:51153480-51153499	None:intergenic	25.0%
!!	TTTTCTTAGCAAGGTTTTAG+GGG	+	chr5_3:51150224-51150243	Msa0816640:intron	30.0%
	TCATCTTATATACAAGAGGA+AGG	+	chr5_3:51150259-51150278	Msa0816640:CDS	30.0%
	TTTATCAACTATTCATGGTG+TGG	+	chr5_3:51150617-51150636	Msa0816640:intron	30.0%
!!!	TTCCTGCTAACCATTTTTTA+TGG	+	chr5_3:51150889-51150908	Msa0816640:intron	30.0%
!!!	TCCTGCTAACCATTTTTTAT+GGG	+	chr5_3:51150890-51150909	Msa0816640:intron	30.0%
	TTGTGATACCCCATAAAAAA+TGG	-	chr5_3:51150902-51150921	None:intergenic	30.0%
	AGACATCTCAAGATATAAAG+TGG	-	chr5_3:51151085-51151104	None:intergenic	30.0%
	CAAATTACATTTGCGTTTAG+AGG	-	chr5_3:51151109-51151128	None:intergenic	30.0%
!!	TGACATTGATCTCATTTTTC+CGG	+	chr5_3:51151136-51151155	Msa0816640:intron	30.0%
	AAAACAACTTGAATCATTGC+CGG	-	chr5_3:51151158-51151177	None:intergenic	30.0%
	GTATTCTTAAGAATAAGGGT+AGG	+	chr5_3:51151196-51151215	Msa0816640:intron	30.0%
!!	TTAACTTGCTTGTTAAAGGA+TGG	+	chr5_3:51151222-51151241	Msa0816640:intron	30.0%
!!	TAACTTGCTTGTTAAAGGAT+GGG	+	chr5_3:51151223-51151242	Msa0816640:intron	30.0%
!	TTTGCAGTTGTTATTGAAGT+TGG	+	chr5_3:51151308-51151327	Msa0816640:intron	30.0%
	AACAGATTGATTGAAACTGT+TGG	+	chr5_3:51151336-51151355	Msa0816640:CDS	30.0%
	TATTCCTAATGAAGATGAGA+AGG	+	chr5_3:51151359-51151378	Msa0816640:CDS	30.0%
	ATTCCTAATGAAGATGAGAA+GGG	+	chr5_3:51151360-51151379	Msa0816640:CDS	30.0%
	TGTTATGTCCATCTGTTATT+TGG	+	chr5_3:51151515-51151534	Msa0816640:intron	30.0%
	CAATTTATTTATGGATCACG+TGG	-	chr5_3:51151680-51151699	None:intergenic	30.0%
	ATCCATAAATAAATTGCCCA+AGG	+	chr5_3:51151684-51151703	Msa0816640:intron	30.0%
!	AAAGCAATGAGATCATTTTC+GGG	+	chr5_3:51151769-51151788	Msa0816640:intron	30.0%
!	AATTTTATGTGAACAGGTCT+TGG	+	chr5_3:51151849-51151868	Msa0816640:intron	30.0%
	GTGACTAATGTAGTCTTTAT+GGG	+	chr5_3:51151898-51151917	Msa0816640:CDS	30.0%
	TAATGTAGTCTTTATGGGAA+TGG	+	chr5_3:51151903-51151922	Msa0816640:CDS	30.0%
	AATGTAGTCTTTATGGGAAT+GGG	+	chr5_3:51151904-51151923	Msa0816640:CDS	30.0%
	TTGCACTAAATAACGTATCA+CGG	-	chr5_3:51152115-51152134	None:intergenic	30.0%
!	GATCAGAATAAAGTTTTGCT+TGG	+	chr5_3:51152232-51152251	Msa0816640:intron	30.0%
	TTCTTGTAGGATGTACAAAT+TGG	+	chr5_3:51152348-51152367	Msa0816640:intron	30.0%
	TCTTGTAGGATGTACAAATT+GGG	+	chr5_3:51152349-51152368	Msa0816640:intron	30.0%
!!!	TCATTTGATGGCTTTTTTAG+TGG	+	chr5_3:51152603-51152622	Msa0816640:intron	30.0%
!	TCCTTTGAATATGCACTTTT+AGG	+	chr5_3:51152815-51152834	Msa0816640:CDS	30.0%
!	ACCTAAAAGTGCATATTCAA+AGG	-	chr5_3:51152819-51152838	None:intergenic	30.0%
!!	TGAATATGCACTTTTAGGTA+CGG	+	chr5_3:51152820-51152839	Msa0816640:intron	30.0%
	GCTAAACTACTACATGAATA+TGG	+	chr5_3:51152977-51152996	Msa0816640:CDS	30.0%
	TCCAAACTTTGTAATTTGGT+AGG	+	chr5_3:51153162-51153181	Msa0816640:intron	30.0%
	ACCTACCAAATTACAAAGTT+TGG	-	chr5_3:51153166-51153185	None:intergenic	30.0%
	TACCAATTATGAAGTGATAG+AGG	+	chr5_3:51153499-51153518	Msa0816640:three_prime_UTR	30.0%
	TAGAAAGCCTTTAGATTCTT+TGG	+	chr5_3:51153635-51153654	Msa0816640:three_prime_UTR	30.0%
	TTTCATAAGAAACAACCTAC+TGG	+	chr5_3:51153672-51153691	Msa0816640:three_prime_UTR	30.0%
	GTAGTAACTAAATTTCCAGT+AGG	-	chr5_3:51153690-51153709	None:intergenic	30.0%
	ATTGTTGGCAATAAGTGATT+AGG	+	chr5_3:51153721-51153740	Msa0816640:three_prime_UTR	30.0%
!	GGGAGATATTTTTCATGTAT+TGG	+	chr5_3:51153769-51153788	Msa0816640:three_prime_UTR	30.0%
!!	TTTTGTTGCTGTTAAAACAC+AGG	+	chr5_3:51153891-51153910	Msa0816640:three_prime_UTR	30.0%
!!!	AAGTTTTTGGAGTTCGGTTT+CGG	-	chr5_3:51150075-51150094	None:intergenic	35.0%
	ATTTACCTGACCAAAGTCAA+GGG	-	chr5_3:51150099-51150118	None:intergenic	35.0%
	AATTTACCTGACCAAAGTCA+AGG	-	chr5_3:51150100-51150119	None:intergenic	35.0%
!!!	TTTCTTAGCAAGGTTTTAGG+GGG	+	chr5_3:51150225-51150244	Msa0816640:intron	35.0%
	AGATATTCAGGAGTTTACTC+AGG	+	chr5_3:51150286-51150305	Msa0816640:CDS	35.0%
	GATATTCAGGAGTTTACTCA+GGG	+	chr5_3:51150287-51150306	Msa0816640:CDS	35.0%
!!	TGTTTCAGTGTAGCATTGAT+AGG	+	chr5_3:51150484-51150503	Msa0816640:intron	35.0%
!!!	GTTGGTTTTAGTGTTGTGTT+TGG	+	chr5_3:51150644-51150663	Msa0816640:intron	35.0%
	TTTCAACACTGTTGTTGCAA+CGG	+	chr5_3:51150791-51150810	Msa0816640:CDS	35.0%
!!!	CCTGCTAACCATTTTTTATG+GGG	+	chr5_3:51150891-51150910	Msa0816640:intron	35.0%
!!	CACAAAAAGAGCATTTCAGA+GGG	-	chr5_3:51151557-51151576	None:intergenic	35.0%
	AAACCTGCTCAACAATTTCA+TGG	-	chr5_3:51151617-51151636	None:intergenic	35.0%
	TGCCTTGGGCAATTTATTTA+TGG	-	chr5_3:51151689-51151708	None:intergenic	35.0%
	TTCAATGTTATCCTGCTTTG+TGG	-	chr5_3:51151831-51151850	None:intergenic	35.0%
	GGTGACTAATGTAGTCTTTA+TGG	+	chr5_3:51151897-51151916	Msa0816640:CDS	35.0%
	TGAACCAATGTTAAACCTGA+AGG	+	chr5_3:51151927-51151946	Msa0816640:CDS	35.0%
	CATTCACCTTATTCAAGCAA+TGG	-	chr5_3:51151969-51151988	None:intergenic	35.0%
	GTATGTAAGTGTGCTAATGT+TGG	+	chr5_3:51152312-51152331	Msa0816640:intron	35.0%
	ACTGACAAGATCAGAATCAA+GGG	-	chr5_3:51152471-51152490	None:intergenic	35.0%
	GATCTTGTCAGTCAAGAATT+AGG	+	chr5_3:51152479-51152498	Msa0816640:intron	35.0%
	TCATTAACCTTACTGTACGA+GGG	-	chr5_3:51152872-51152891	None:intergenic	35.0%
	TTCATTAACCTTACTGTACG+AGG	-	chr5_3:51152873-51152892	None:intergenic	35.0%
	CAAACATAATGACAGGAAAG+TGG	-	chr5_3:51152914-51152933	None:intergenic	35.0%
	GAGCCTTCTATTGGATTAAA+AGG	-	chr5_3:51153028-51153047	None:intergenic	35.0%
	TATCATACTGCTTTCCTGTA+AGG	-	chr5_3:51153064-51153083	None:intergenic	35.0%
!	GCAAGCATTTGTTAATGCTT+TGG	+	chr5_3:51153186-51153205	Msa0816640:CDS	35.0%
	CAATTTCCAGAAAACACCTT+TGG	+	chr5_3:51153285-51153304	Msa0816640:CDS	35.0%
	GTTTGGACTAAAACAATGAG+AGG	+	chr5_3:51153445-51153464	Msa0816640:three_prime_UTR	35.0%
	CACCTCTATCACTTCATAAT+TGG	-	chr5_3:51153504-51153523	None:intergenic	35.0%
	GAAAGTTGACATTGTAACTC+AGG	-	chr5_3:51153540-51153559	None:intergenic	35.0%
!!!	AGAAGCATCTATGTGGTTTT+TGG	+	chr5_3:51153580-51153599	Msa0816640:three_prime_UTR	35.0%
!!!	GGTTTTTGGCACTAATTTTG+TGG	+	chr5_3:51153594-51153613	Msa0816640:three_prime_UTR	35.0%
	TTAGCTGCCAAAGAATCTAA+AGG	-	chr5_3:51153645-51153664	None:intergenic	35.0%
!	TTAGGACTTGGTAGTTCATA+TGG	+	chr5_3:51153739-51153758	Msa0816640:three_prime_UTR	35.0%
	TTAAAACACAGGCGTAGTTT+GGG	+	chr5_3:51153902-51153921	Msa0816640:three_prime_UTR	35.0%
	AGAAGAAGTTGCGAAGGAAA+TGG	-	chr5_3:51150009-51150028	None:intergenic	40.0%
!!!	AAGGGCAAGTTTTTGGAGTT+CGG	-	chr5_3:51150081-51150100	None:intergenic	40.0%
!	CAAAGTCAAGGGCAAGTTTT+TGG	-	chr5_3:51150088-51150107	None:intergenic	40.0%
	CAAAAACTTGCCCTTGACTT+TGG	+	chr5_3:51150086-51150105	Msa0816640:CDS	40.0%
	GAGGAAGGTCAAAGATATTC+AGG	+	chr5_3:51150274-51150293	Msa0816640:CDS	40.0%
	GTGATTGAATGTAGCCACAT+GGG	-	chr5_3:51150591-51150610	None:intergenic	40.0%
	AGTGATTGAATGTAGCCACA+TGG	-	chr5_3:51150592-51150611	None:intergenic	40.0%
!!	TATTCATGGTGTGGTGTTGT+TGG	+	chr5_3:51150626-51150645	Msa0816640:intron	40.0%
!!!	TTTTCGATGTGGTGTTGTTG+TGG	+	chr5_3:51150690-51150709	Msa0816640:intron	40.0%
!	GCAACAACAGTGTTGAAAAC+TGG	-	chr5_3:51150789-51150808	None:intergenic	40.0%
	CCCCATAAAAAATGGTTAGC+AGG	-	chr5_3:51150894-51150913	None:intergenic	40.0%
	GAACCCTTCTCATCTTCATT+AGG	-	chr5_3:51151366-51151385	None:intergenic	40.0%
!!	GCACAAAAAGAGCATTTCAG+AGG	-	chr5_3:51151558-51151577	None:intergenic	40.0%
!	AAATGCTCTTTTTGTGCCAC+TGG	+	chr5_3:51151561-51151580	Msa0816640:CDS	40.0%
!!	TCTTTTTGTGCCACTGGAAA+AGG	+	chr5_3:51151567-51151586	Msa0816640:CDS	40.0%
!!	CTTTTTGTGCCACTGGAAAA+GGG	+	chr5_3:51151568-51151587	Msa0816640:CDS	40.0%
!!	TTTTTGTGCCACTGGAAAAG+GGG	+	chr5_3:51151569-51151588	Msa0816640:CDS	40.0%
	TTTCTGTTTCACCACAAAGC+AGG	+	chr5_3:51151817-51151836	Msa0816640:intron	40.0%
!	ACTGCCTTCAGGTTTAACAT+TGG	-	chr5_3:51151934-51151953	None:intergenic	40.0%
	TTAAACCTGAAGGCAGTACT+TGG	+	chr5_3:51151937-51151956	Msa0816640:CDS	40.0%
	TAAACCTGAAGGCAGTACTT+GGG	+	chr5_3:51151938-51151957	Msa0816640:CDS	40.0%
	AGTGTGCTAATGTTGGATTC+TGG	+	chr5_3:51152319-51152338	Msa0816640:intron	40.0%
	TGCTAATGTTGGATTCTGGT+TGG	+	chr5_3:51152323-51152342	Msa0816640:intron	40.0%
!	GCTAATGTTGGATTCTGGTT+GGG	+	chr5_3:51152324-51152343	Msa0816640:intron	40.0%
!	TATTGTATTTGGAACCCCCA+CGG	-	chr5_3:51152397-51152416	None:intergenic	40.0%
	TTCAATCCACTTTAGCTGTG+AGG	+	chr5_3:51152439-51152458	Msa0816640:intron	40.0%
	CAATTACCTCACAGCTAAAG+TGG	-	chr5_3:51152448-51152467	None:intergenic	40.0%
	GACTGACAAGATCAGAATCA+AGG	-	chr5_3:51152472-51152491	None:intergenic	40.0%
	TTCGTTCGTTGTTCATACTC+CGG	+	chr5_3:51152544-51152563	Msa0816640:intron	40.0%
	GGCTGATGGAAGATACAAAA+GGG	-	chr5_3:51152679-51152698	None:intergenic	40.0%
	AGGCTGATGGAAGATACAAA+AGG	-	chr5_3:51152680-51152699	None:intergenic	40.0%
	ATGCTCCTAACCAGAAACTA+AGG	+	chr5_3:51152702-51152721	Msa0816640:intron	40.0%
	TGCTCCTAACCAGAAACTAA+GGG	+	chr5_3:51152703-51152722	Msa0816640:intron	40.0%
	CAATTGTCTCCCTTAGTTTC+TGG	-	chr5_3:51152715-51152734	None:intergenic	40.0%
	AATGGGTAAGACTTTGCACT+TGG	-	chr5_3:51152740-51152759	None:intergenic	40.0%
	AAGTGCAAAGTCTTACCCAT+TGG	+	chr5_3:51152739-51152758	Msa0816640:CDS	40.0%
	CTTGCATAAGTGCATCCAAT+GGG	-	chr5_3:51152757-51152776	None:intergenic	40.0%
	TCTTGCATAAGTGCATCCAA+TGG	-	chr5_3:51152758-51152777	None:intergenic	40.0%
	AGCTGCTCAAACATAATGAC+AGG	-	chr5_3:51152921-51152940	None:intergenic	40.0%
	TGTCATTATGTTTGAGCAGC+TGG	+	chr5_3:51152920-51152939	Msa0816640:intron	40.0%
	CTACTACATGAATATGGCAG+TGG	+	chr5_3:51152983-51153002	Msa0816640:CDS	40.0%
	CAAAACTCAGAGCCTTCTAT+TGG	-	chr5_3:51153037-51153056	None:intergenic	40.0%
	ATACTGCTTTCCTGTAAGGA+CGG	-	chr5_3:51153060-51153079	None:intergenic	40.0%
	CACACACAATGTCAGTTTCA+TGG	+	chr5_3:51153376-51153395	Msa0816640:three_prime_UTR	40.0%
	TTCTTGCTAGCAGAGAAATG+TGG	+	chr5_3:51153557-51153576	Msa0816640:three_prime_UTR	40.0%
!!	AATGTGGAGAAGCATCTATG+TGG	+	chr5_3:51153573-51153592	Msa0816640:three_prime_UTR	40.0%
!	GCACTAATTTTGTGGTATGC+AGG	+	chr5_3:51153602-51153621	Msa0816640:three_prime_UTR	40.0%
!	AATTTTGTGGTATGCAGGCT+TGG	+	chr5_3:51153607-51153626	Msa0816640:three_prime_UTR	40.0%
	TAGATTCTTTGGCAGCTAAG+AGG	+	chr5_3:51153646-51153665	Msa0816640:three_prime_UTR	40.0%
	CTACCACGAGCAAATATTGT+TGG	+	chr5_3:51153706-51153725	Msa0816640:three_prime_UTR	40.0%
	TTGCCAACAATATTTGCTCG+TGG	-	chr5_3:51153712-51153731	None:intergenic	40.0%
	GGCAATAAGTGATTAGGACT+TGG	+	chr5_3:51153727-51153746	Msa0816640:three_prime_UTR	40.0%
	GTAGTTCATATGGTGCACTT+GGG	+	chr5_3:51153749-51153768	Msa0816640:three_prime_UTR	40.0%
!	ACGGTCAGATTTTTACAAGC+TGG	-	chr5_3:51153834-51153853	None:intergenic	40.0%
	GTTAAAACACAGGCGTAGTT+TGG	+	chr5_3:51153901-51153920	Msa0816640:three_prime_UTR	40.0%
	AGAAGTTGCGAAGGAAATGG+CGG	-	chr5_3:51150006-51150025	None:intergenic	45.0%
	AGGAGAAGAAGAAGTTGCGA+AGG	-	chr5_3:51150015-51150034	None:intergenic	45.0%
	CATTGATAGGACATTGACGC+AGG	+	chr5_3:51150497-51150516	Msa0816640:intron	45.0%
	TCGTGTGAATGACACTGACA+CGG	-	chr5_3:51150562-51150581	None:intergenic	45.0%
!	CAGTGTCATTCACACGACAT+TGG	+	chr5_3:51150565-51150584	Msa0816640:intron	45.0%
	AGCTCATTCCTAAATGCCTG+AGG	-	chr5_3:51150735-51150754	None:intergenic	45.0%
	GGCATTTAGGAATGAGCTTG+AGG	+	chr5_3:51150737-51150756	Msa0816640:CDS	45.0%
	AACACTGTTGTTGCAACGGA+TGG	+	chr5_3:51150795-51150814	Msa0816640:CDS	45.0%
	TGCAACGGATGGAACTGTTA+AGG	+	chr5_3:51150806-51150825	Msa0816640:CDS	45.0%
!!	TTTTGTGCCACTGGAAAAGG+GGG	+	chr5_3:51151570-51151589	Msa0816640:CDS	45.0%
!!	GGGGCTTTTCAAGGAATCTT+CGG	+	chr5_3:51151589-51151608	Msa0816640:CDS	45.0%
	GAGCCATGAAATTGTTGAGC+AGG	+	chr5_3:51151611-51151630	Msa0816640:CDS	45.0%
	AAACCTGAAGGCAGTACTTG+GGG	+	chr5_3:51151939-51151958	Msa0816640:CDS	45.0%
	GGCACACCATTGCTTGAATA+AGG	+	chr5_3:51151960-51151979	Msa0816640:CDS	45.0%
	AACGTATCACGGAGCAATAG+CGG	-	chr5_3:51152104-51152123	None:intergenic	45.0%
!	ATTCTGGTTGGGCTTCTTGT+AGG	+	chr5_3:51152335-51152354	Msa0816640:intron	45.0%
	AATGATGACTATCTCCACCG+TGG	+	chr5_3:51152377-51152396	Msa0816640:intron	45.0%
	ATGATGACTATCTCCACCGT+GGG	+	chr5_3:51152378-51152397	Msa0816640:intron	45.0%
	AATCAGACCAGATCAGACAC+CGG	-	chr5_3:51152566-51152585	None:intergenic	45.0%
!	GTCTGATCTGGTCTGATTTG+TGG	+	chr5_3:51152568-51152587	Msa0816640:intron	45.0%
!!	GTTTCTGGTTAGGAGCATGT+AGG	-	chr5_3:51152700-51152719	None:intergenic	45.0%
!!	GTCTCCCTTAGTTTCTGGTT+AGG	-	chr5_3:51152710-51152729	None:intergenic	45.0%
	TGCACTTATGCAAGACTGCA+AGG	+	chr5_3:51152763-51152782	Msa0816640:CDS	45.0%
	ACTTCCGCGAAACAAGTAGA+CGG	+	chr5_3:51152789-51152808	Msa0816640:CDS	45.0%
	AAACCCGTCTACTTGTTTCG+CGG	-	chr5_3:51152796-51152815	None:intergenic	45.0%
	AGGGAGGAACAAGAATGTGT+TGG	-	chr5_3:51152853-51152872	None:intergenic	45.0%
	TTAACCTTACTGTACGAGGG+AGG	-	chr5_3:51152869-51152888	None:intergenic	45.0%
	AGCAGCTGGAATCAATGACT+CGG	+	chr5_3:51152934-51152953	Msa0816640:intron	45.0%
	CAGATTGTTGTCAGAGTCTG+CGG	-	chr5_3:51153311-51153330	None:intergenic	45.0%
	AAGGTCCATGTTTGGAAGCT+GGG	-	chr5_3:51153338-51153357	None:intergenic	45.0%
	GTAACTGCAAGGTCCATGTT+TGG	-	chr5_3:51153346-51153365	None:intergenic	45.0%
	AACATGGACCTTGCAGTTAC+AGG	+	chr5_3:51153346-51153365	Msa0816640:CDS	45.0%
	GCAATCAACCTGTAACTGCA+AGG	-	chr5_3:51153357-51153376	None:intergenic	45.0%
	ATTGTAACTCAGGCAGCAAC+AGG	-	chr5_3:51153530-51153549	None:intergenic	45.0%
	GGTAGTTCATATGGTGCACT+TGG	+	chr5_3:51153748-51153767	Msa0816640:three_prime_UTR	45.0%
	TGGGATGCACATCCTTTAAG+TGG	+	chr5_3:51153921-51153940	Msa0816640:three_prime_UTR	45.0%
	TGAGACTGAGGAATTGTGCG+AGG	-	chr5_3:51150035-51150054	None:intergenic	50.0%
	TCGCACAATTCCTCAGTCTC+AGG	+	chr5_3:51150034-51150053	Msa0816640:CDS	50.0%
	GTTCGGTTTCGGATAAGCCA+AGG	-	chr5_3:51150064-51150083	None:intergenic	50.0%
	ACTTGCCCTTGACTTTGGTC+AGG	+	chr5_3:51150091-51150110	Msa0816640:CDS	50.0%
!!	CGTGTCGGTTGTTCGAACAT+CGG	-	chr5_3:51150387-51150406	None:intergenic	50.0%
	TGATAGGACATTGACGCAGG+TGG	+	chr5_3:51150500-51150519	Msa0816640:intron	50.0%
	TTGCAGTGCCTCAGGCATTT+AGG	+	chr5_3:51150724-51150743	Msa0816640:intron	50.0%
	AGGAATGAGCTTGAGGAAGC+TGG	+	chr5_3:51150744-51150763	Msa0816640:CDS	50.0%
	ATGAGCTTGAGGAAGCTGGA+TGG	+	chr5_3:51150748-51150767	Msa0816640:CDS	50.0%
	GTGATGAGACACATGCTGTC+AGG	-	chr5_3:51151395-51151414	None:intergenic	50.0%
	GACAGCATGTGTCTCATCAC+AGG	+	chr5_3:51151395-51151414	Msa0816640:CDS	50.0%
!	TGAAAAGCCCCCTTTTCCAG+TGG	-	chr5_3:51151580-51151599	None:intergenic	50.0%
!	CTGGAAAAGGGGGCTTTTCA+AGG	+	chr5_3:51151580-51151599	Msa0816640:CDS	50.0%
	TGAACAGGTCTTGGCCATTG+AGG	+	chr5_3:51151858-51151877	Msa0816640:intron	50.0%
	GCGTTTGAACACCTCCTCAA+TGG	-	chr5_3:51151875-51151894	None:intergenic	50.0%
!!	TTGAGGAGGTGTTCAAACGC+AGG	+	chr5_3:51151875-51151894	Msa0816640:CDS	50.0%
!!	TGAGGAGGTGTTCAAACGCA+GGG	+	chr5_3:51151876-51151895	Msa0816640:CDS	50.0%
	TGATGACTATCTCCACCGTG+GGG	+	chr5_3:51152379-51152398	Msa0816640:intron	50.0%
	TCATACTCCGGTGTCTGATC+TGG	+	chr5_3:51152556-51152575	Msa0816640:intron	50.0%
!	GTTAGGAGCATGTAGGCTGA+TGG	-	chr5_3:51152693-51152712	None:intergenic	50.0%
	CTTCCGCGAAACAAGTAGAC+GGG	+	chr5_3:51152790-51152809	Msa0816640:CDS	50.0%
	TGTTCCTCCCTCGTACAGTA+AGG	+	chr5_3:51152862-51152881	Msa0816640:intron	50.0%
!	CTGAGTTTTGCCGTCCTTAC+AGG	+	chr5_3:51153047-51153066	Msa0816640:CDS	50.0%
!!	TCTGGTTTGGCGTACACTAG+TGG	-	chr5_3:51153227-51153246	None:intergenic	50.0%
	ACTAGTGTACGCCAAACCAG+AGG	+	chr5_3:51153226-51153245	Msa0816640:CDS	50.0%
	TTACGTCCAGTCCTCTGGTT+TGG	-	chr5_3:51153240-51153259	None:intergenic	50.0%
	TGCATTTACGTCCAGTCCTC+TGG	-	chr5_3:51153245-51153264	None:intergenic	50.0%
	CTGGACGTAAATGCAGCTTG+TGG	+	chr5_3:51153250-51153269	Msa0816640:CDS	50.0%
	ATGCAGCTTGTGGTCAGCTA+CGG	+	chr5_3:51153260-51153279	Msa0816640:CDS	50.0%
	CGCAGACTCTGACAACAATC+TGG	+	chr5_3:51153309-51153328	Msa0816640:CDS	50.0%
	GGAATCCCAGCTTCCAAACA+TGG	+	chr5_3:51153330-51153349	Msa0816640:CDS	50.0%
	CAAGGTCCATGTTTGGAAGC+TGG	-	chr5_3:51153339-51153358	None:intergenic	50.0%
	CCAAGGAGAACCTGAGACTG+AGG	-	chr5_3:51150047-51150066	None:intergenic	55.0%
	CCTCAGTCTCAGGTTCTCCT+TGG	+	chr5_3:51150044-51150063	Msa0816640:CDS	55.0%
	AACCGACACGACACTGACAC+AGG	+	chr5_3:51150397-51150416	Msa0816640:intron	55.0%
	CACCTGTGTCAGTGTCGTGT+CGG	-	chr5_3:51150402-51150421	None:intergenic	55.0%
	TCACACGACATTGGCCCATG+TGG	+	chr5_3:51150574-51150593	Msa0816640:intron	55.0%
	TGCTTGCATTGCAGTGCCTC+AGG	+	chr5_3:51150716-51150735	Msa0816640:intron	55.0%
	GCTTGAGGAAGCTGGATGGA+AGG	+	chr5_3:51150752-51150771	Msa0816640:CDS	55.0%
	GAGGAAGCTGGATGGAAGGT+CGG	+	chr5_3:51150756-51150775	Msa0816640:CDS	55.0%
	AAGCTGGATGGAAGGTCGGA+CGG	+	chr5_3:51150760-51150779	Msa0816640:CDS	55.0%
	ACAGGTCTTGGCCATTGAGG+AGG	+	chr5_3:51151861-51151880	Msa0816640:intron	55.0%
	GATGACTATCTCCACCGTGG+GGG	+	chr5_3:51152380-51152399	Msa0816640:intron	55.0%
!	TGTATTTGGAACCCCCACGG+TGG	-	chr5_3:51152394-51152413	None:intergenic	55.0%
	TGTACGCCAAACCAGAGGAC+TGG	+	chr5_3:51153231-51153250	Msa0816640:CDS	55.0%
!!!	CTGCGGCCAAAGGTGTTTTC+TGG	-	chr5_3:51153294-51153313	None:intergenic	55.0%
	TTGTCAGAGTCTGCGGCCAA+AGG	-	chr5_3:51153304-51153323	None:intergenic	55.0%
!	GTGCGAGGGGCATGGAATAG+TGG	-	chr5_3:51149953-51149972	None:intergenic	60.0%
	GAAATGGCGGCACGTGTGTG+AGG	-	chr5_3:51149993-51150012	None:intergenic	60.0%
	CGACACGACACTGACACAGG+TGG	+	chr5_3:51150400-51150419	Msa0816640:intron	60.0%
!	GTGCCCCAAGTACTGCCTTC+AGG	-	chr5_3:51151945-51151964	None:intergenic	60.0%
	TTCCATGCCCCTCGCACGTG+CGG	+	chr5_3:51149956-51149975	Msa0816640:CDS	65.0%
	GCGGCACGTGTGTGAGGTGA+AGG	-	chr5_3:51149987-51150006	None:intergenic	65.0%
	AGGACGCACCGCACGTGCGA+GGG	-	chr5_3:51149967-51149986	None:intergenic	70.0%
	AAGGACGCACCGCACGTGCG+AGG	-	chr5_3:51149968-51149987	None:intergenic	70.0%
	CACCGCACGTGCGAGGGGCA+TGG	-	chr5_3:51149961-51149980	None:intergenic	75.0%
	GGACGCACCGCACGTGCGAG+GGG	-	chr5_3:51149966-51149985	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	51149904	51153953	51149904	ID=Msa0816640;Name=Msa0816640
chr5_3	mRNA	51149904	51153953	51149904	ID=Msa0816640-mRNA-1;Parent=Msa0816640;Name=Msa0816640-mRNA-1;_AED=0.15;_eAED=0.15;_QI=29\|0.87\|0.88\|1\|1\|1\|9\|581\|368
chr5_3	exon	51149904	51150112	51149904	ID=Msa0816640-mRNA-1:exon:15353;Parent=Msa0816640-mRNA-1
chr5_3	exon	51150233	51150308	51150233	ID=Msa0816640-mRNA-1:exon:15354;Parent=Msa0816640-mRNA-1
chr5_3	exon	51150730	51150827	51150730	ID=Msa0816640-mRNA-1:exon:15355;Parent=Msa0816640-mRNA-1
chr5_3	exon	51151315	51151416	51151315	ID=Msa0816640-mRNA-1:exon:15356;Parent=Msa0816640-mRNA-1
chr5_3	exon	51151546	51151632	51151546	ID=Msa0816640-mRNA-1:exon:15357;Parent=Msa0816640-mRNA-1
chr5_3	exon	51151865	51151981	51151865	ID=Msa0816640-mRNA-1:exon:15358;Parent=Msa0816640-mRNA-1
chr5_3	exon	51152716	51152836	51152716	ID=Msa0816640-mRNA-1:exon:15359;Parent=Msa0816640-mRNA-1
chr5_3	exon	51152939	51153075	51152939	ID=Msa0816640-mRNA-1:exon:15360;Parent=Msa0816640-mRNA-1
chr5_3	exon	51153184	51153953	51153184	ID=Msa0816640-mRNA-1:exon:15361;Parent=Msa0816640-mRNA-1
chr5_3	five_prime_UTR	51149904	51149932	51149904	ID=Msa0816640-mRNA-1:five_prime_utr;Parent=Msa0816640-mRNA-1
chr5_3	CDS	51149933	51150112	51149933	ID=Msa0816640-mRNA-1:cds;Parent=Msa0816640-mRNA-1
chr5_3	CDS	51150233	51150308	51150233	ID=Msa0816640-mRNA-1:cds;Parent=Msa0816640-mRNA-1
chr5_3	CDS	51150730	51150827	51150730	ID=Msa0816640-mRNA-1:cds;Parent=Msa0816640-mRNA-1
chr5_3	CDS	51151315	51151416	51151315	ID=Msa0816640-mRNA-1:cds;Parent=Msa0816640-mRNA-1
chr5_3	CDS	51151546	51151632	51151546	ID=Msa0816640-mRNA-1:cds;Parent=Msa0816640-mRNA-1
chr5_3	CDS	51151865	51151981	51151865	ID=Msa0816640-mRNA-1:cds;Parent=Msa0816640-mRNA-1
chr5_3	CDS	51152716	51152836	51152716	ID=Msa0816640-mRNA-1:cds;Parent=Msa0816640-mRNA-1
chr5_3	CDS	51152939	51153075	51152939	ID=Msa0816640-mRNA-1:cds;Parent=Msa0816640-mRNA-1
chr5_3	CDS	51153184	51153372	51153184	ID=Msa0816640-mRNA-1:cds;Parent=Msa0816640-mRNA-1
chr5_3	three_prime_UTR	51153373	51153953	51153373	ID=Msa0816640-mRNA-1:three_prime_utr;Parent=Msa0816640-mRNA-1

Gene Sequence

>Msa0816640

ATGATGTCTCTGCTTCACCACTATTCCATGCCCCTCGCACGTGCGGTGCGTCCTTCACCTCACACACGTGCCGCCATTTCCTTCGCAACTTCTTCTTCTCCTCGCACAATTCCTCAGTCTCAGGTTCTCCTTGGCTTATCCGAAACCGAACTCCAAAAACTTGCCCTTGACTTTGGTCAGCAAGGTTTTAGGGGGAAGCAACTTTATCATCTTATATACAAGAGGAAGGTCAAAGATATTCAGGAGTTTACTCAGGTGCCTCAGGCATTTAGGAATGAGCTTGAGGAAGCTGGATGGAAGGTCGGACGGTCTCCAGTTTTCAACACTGTTGTTGCAACGGATGGAACTGTTAAGTTGTTATTGAAGTTGGAAGACAACAGATTGATTGAAACTGTTGGTATTCCTAATGAAGATGAGAAGGGTTCAGTTCGCCTGACAGCATGTGTCTCATCACAGGTAGGTTGCCCTCTGAAATGCTCTTTTTGTGCCACTGGAAAAGGGGGCTTTTCAAGGAATCTTCGGAGCCATGAAATTGTTGAGCAGGTCTTGGCCATTGAGGAGGTGTTCAAACGCAGGGTGACTAATGTAGTCTTTATGGGAATGGGTGAACCAATGTTAAACCTGAAGGCAGTACTTGGGGCACACCATTGCTTGAATAAGAAACTAAGGGAGACAATTGTTCCAAGTGCAAAGTCTTACCCATTGGATGCACTTATGCAAGACTGCAAGGAATACTTCCGCGAAACAAGTAGACGGGTTTCCTTTGAATATGCACTTTTAGCTGGAATCAATGACTCGGTAGACAATGCAGTAGAACTAGCTAAACTACTACATGAATATGGCAGTGGTTATCATGTCAATCTTATACCTTTTAATCCAATAGAAGGCTCTGAGTTTTGCCGTCCTTACAGGAAAGCAGTGCAAGCATTTGTTAATGCTTTGGAGTCGTCTAAAATAACCACTAGTGTACGCCAAACCAGAGGACTGGACGTAAATGCAGCTTGTGGTCAGCTACGGAACAATTTCCAGAAAACACCTTTGGCCGCAGACTCTGACAACAATCTGGAATCCCAGCTTCCAAACATGGACCTTGCAGTTACAGGTTGA

Protein sequence

>Msa0816640

MMSLLHHYSMPLARAVRPSPHTRAAISFATSSSPRTIPQSQVLLGLSETELQKLALDFGQQGFRGKQLYHLIYKRKVKDIQEFTQVPQAFRNELEEAGWKVGRSPVFNTVVATDGTVKLLLKLEDNRLIETVGIPNEDEKGSVRLTACVSSQVGCPLKCSFCATGKGGFSRNLRSHEIVEQVLAIEEVFKRRVTNVVFMGMGEPMLNLKAVLGAHHCLNKKLRETIVPSAKSYPLDALMQDCKEYFRETSRRVSFEYALLAGINDSVDNAVELAKLLHEYGSGYHVNLIPFNPIEGSEFCRPYRKAVQAFVNALESSKITTSVRQTRGLDVNAACGQLRNNFQKTPLAADSDNNLESQLPNMDLAVTG*