AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061819


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061819.t1 MTR_5g093240 90.123 81 5 1 1 81 1 78 6.47e-47 145
MS.gene061819.t1 MTR_5g093240 90.123 81 5 1 1 81 1 78 2.68e-46 143
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 27 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTTCAGTAAGTTCATTA+AGG 0.284385 5.1:-74304003 MS.gene061819:CDS
CGCAGCAGCAACATTATCTA+AGG 0.361910 5.1:-74304090 MS.gene061819:CDS
GCAGCAACAACAATGATATC+TGG 0.364461 5.1:+74303968 None:intergenic
GCACAACACAAAACTACTTT+GGG 0.366214 5.1:-74304062 MS.gene061819:CDS
ATGGATCGGCGGGTTGTGGC+AGG 0.389363 5.1:+74303939 None:intergenic
GAAGCAATGAAGAGGGGAGG+TGG 0.407582 5.1:+74304134 None:intergenic
CTATGTGGCATGGTCTTGCT+TGG 0.417895 5.1:+74304034 None:intergenic
AGCACAACACAAAACTACTT+TGG 0.452055 5.1:-74304063 MS.gene061819:CDS
CAATGATATCTGGAATTGCC+TGG 0.462076 5.1:+74303978 None:intergenic
CTTACTGGGAGAAAAGTAGA+TGG 0.479013 5.1:+74303920 None:intergenic
GTAGATGGATCGGCGGGTTG+TGG 0.490348 5.1:+74303935 None:intergenic
CTTAGATAATGTTGCTGCTG+CGG 0.540896 5.1:+74304091 None:intergenic
TGCATGAGAAGCAATGAAGA+GGG 0.563172 5.1:+74304127 None:intergenic
TGGATCGGCGGGTTGTGGCA+GGG 0.568272 5.1:+74303940 None:intergenic
AGAAAAGTAGATGGATCGGC+GGG 0.588206 5.1:+74303929 None:intergenic
AATGATATCTGGAATTGCCT+GGG 0.588413 5.1:+74303979 None:intergenic
GAAGAAAAGTGCTTACTATG+TGG 0.588606 5.1:+74304019 None:intergenic
TGGGAGAAAAGTAGATGGAT+CGG 0.598368 5.1:+74303925 None:intergenic
GCAGCAACATTATCTAAGGA+TGG 0.606428 5.1:-74304086 MS.gene061819:CDS
AGTAAGTTCATTAAGGACCC+AGG 0.609846 5.1:-74303996 MS.gene061819:CDS
AAAGTGCTTACTATGTGGCA+TGG 0.619717 5.1:+74304024 None:intergenic
GGGGAGGTGGAGACAAGTCA+TGG 0.642574 5.1:+74304147 None:intergenic
ATGCATGAGAAGCAATGAAG+AGG 0.647708 5.1:+74304126 None:intergenic
TGAGAAGCAATGAAGAGGGG+AGG 0.663602 5.1:+74304131 None:intergenic
GAGAAAAGTAGATGGATCGG+CGG 0.670606 5.1:+74303928 None:intergenic
GCATGAGAAGCAATGAAGAG+GGG 0.705476 5.1:+74304128 None:intergenic
CACAACACAAAACTACTTTG+GGG 0.712340 5.1:-74304061 MS.gene061819:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTATTTTTTTTTTACACT+AGG - chr5.1:74304137-74304156 MS.gene061819:CDS 15.0%
! GTTCATTATGTCATATTTCA+TGG - chr5.1:74304107-74304126 MS.gene061819:CDS 25.0%
! TTTCTTCAGTAAGTTCATTA+AGG - chr5.1:74303979-74303998 MS.gene061819:CDS 25.0%
AGCACAACACAAAACTACTT+TGG - chr5.1:74303919-74303938 MS.gene061819:intron 35.0%
CACAACACAAAACTACTTTG+GGG - chr5.1:74303921-74303940 MS.gene061819:intron 35.0%
GCACAACACAAAACTACTTT+GGG - chr5.1:74303920-74303939 MS.gene061819:intron 35.0%
! AATGATATCTGGAATTGCCT+GGG + chr5.1:74304006-74304025 None:intergenic 35.0%
!! GAAGAAAAGTGCTTACTATG+TGG + chr5.1:74303966-74303985 None:intergenic 35.0%
AGAAAAGAACCGAACCTTAC+TGG + chr5.1:74304080-74304099 None:intergenic 40.0%
AGTAAGTTCATTAAGGACCC+AGG - chr5.1:74303986-74304005 MS.gene061819:CDS 40.0%
ATGCATGAGAAGCAATGAAG+AGG + chr5.1:74303859-74303878 None:intergenic 40.0%
CTTACTGGGAGAAAAGTAGA+TGG + chr5.1:74304065-74304084 None:intergenic 40.0%
CTTAGATAATGTTGCTGCTG+CGG + chr5.1:74303894-74303913 None:intergenic 40.0%
GAAAAGAACCGAACCTTACT+GGG + chr5.1:74304079-74304098 None:intergenic 40.0%
GCAGCAACAACAATGATATC+TGG + chr5.1:74304017-74304036 None:intergenic 40.0%
GCAGCAACATTATCTAAGGA+TGG - chr5.1:74303896-74303915 MS.gene061819:intron 40.0%
TGCATGAGAAGCAATGAAGA+GGG + chr5.1:74303858-74303877 None:intergenic 40.0%
TGGGAGAAAAGTAGATGGAT+CGG + chr5.1:74304060-74304079 None:intergenic 40.0%
! AAAGTGCTTACTATGTGGCA+TGG + chr5.1:74303961-74303980 None:intergenic 40.0%
! ACTTTTCTCCCAGTAAGGTT+CGG - chr5.1:74304068-74304087 MS.gene061819:CDS 40.0%
! CAATGATATCTGGAATTGCC+TGG + chr5.1:74304007-74304026 None:intergenic 40.0%
!! CATCTACTTTTCTCCCAGTA+AGG - chr5.1:74304063-74304082 MS.gene061819:CDS 40.0%
AGAAAAGTAGATGGATCGGC+GGG + chr5.1:74304056-74304075 None:intergenic 45.0%
CGCAGCAGCAACATTATCTA+AGG - chr5.1:74303892-74303911 MS.gene061819:intron 45.0%
GAGAAAAGTAGATGGATCGG+CGG + chr5.1:74304057-74304076 None:intergenic 45.0%
GCATGAGAAGCAATGAAGAG+GGG + chr5.1:74303857-74303876 None:intergenic 45.0%
TGAGAAGCAATGAAGAGGGG+AGG + chr5.1:74303854-74303873 None:intergenic 50.0%
! CTATGTGGCATGGTCTTGCT+TGG + chr5.1:74303951-74303970 None:intergenic 50.0%
GAAGCAATGAAGAGGGGAGG+TGG + chr5.1:74303851-74303870 None:intergenic 55.0%
!! GTAGATGGATCGGCGGGTTG+TGG + chr5.1:74304050-74304069 None:intergenic 60.0%
!! ATGGATCGGCGGGTTGTGGC+AGG + chr5.1:74304046-74304065 None:intergenic 65.0%
!! TGGATCGGCGGGTTGTGGCA+GGG + chr5.1:74304045-74304064 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 74303837 74304167 74303837 ID=MS.gene061819
chr5.1 mRNA 74303837 74304167 74303837 ID=MS.gene061819.t1;Parent=MS.gene061819
chr5.1 exon 74303925 74304167 74303925 ID=MS.gene061819.t1.exon1;Parent=MS.gene061819.t1
chr5.1 CDS 74303925 74304167 74303925 ID=cds.MS.gene061819.t1;Parent=MS.gene061819.t1
chr5.1 exon 74303837 74303845 74303837 ID=MS.gene061819.t1.exon2;Parent=MS.gene061819.t1
chr5.1 CDS 74303837 74303845 74303837 ID=cds.MS.gene061819.t1;Parent=MS.gene061819.t1
Gene Sequence

>MS.gene061819

ATGACTTGTCTCCACCTCCCCTCTTCATTGCTTCTCATGCATACAAAACTCAAACCGCAGCAGCAACATTATCTAAGGATGGAGCACAACACAAAACTACTTTGGGGAGAACCAAGCAAGACCATGCCACATAGTAAGCACTTTTCTTCAGTAAGTTCATTAAGGACCCAGGCAATTCCAGATATCATTGTTGTTGCTGCTACTTCCCTGCCACAACCCGCCGATCCATCTACTTTTCTCCCAGTAAGGTTCGGTTCTTTTCTTTCACTTGTTCATTATGTCATATTTCATGGTCATGTATAGTATTTTTTTTTTACACTAGGAGATATAA

Protein sequence

>MS.gene061819.t1

MTCLHLPSSLLLMHTKLKPQQQHYLRMEHNTKLLWGEPSKTMPHSKHFSSVSSLRTQAIPDIIVVAATSLPQPADPSTFLPEI