Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34383.t1 | XP_013469761.1 | 97.3 | 187 | 5 | 0 | 1 | 187 | 1 | 187 | 4.40E-107 | 397.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34383.t1 | D7T737 | 83.0 | 188 | 31 | 1 | 1 | 187 | 1 | 188 | 1.4e-93 | 343.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34383.t1 | I3SWK2 | 97.3 | 187 | 5 | 0 | 1 | 187 | 1 | 187 | 3.2e-107 | 397.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene34383 | 0.858034 | 1.09E-62 | -1.69E-46 |
| MS.gene049222 | MS.gene34383 | 0.857982 | 1.13E-62 | -1.69E-46 |
| MS.gene049232 | MS.gene34383 | 0.860861 | 1.54E-63 | -1.69E-46 |
| MS.gene049285 | MS.gene34383 | 0.829297 | 5.57E-55 | -1.69E-46 |
| MS.gene049320 | MS.gene34383 | 0.850392 | 1.75E-60 | -1.69E-46 |
| MS.gene049396 | MS.gene34383 | 0.815175 | 1.06E-51 | -1.69E-46 |
| MS.gene049609 | MS.gene34383 | 0.844907 | 5.65E-59 | -1.69E-46 |
| MS.gene049633 | MS.gene34383 | 0.847986 | 8.17E-60 | -1.69E-46 |
| MS.gene049656 | MS.gene34383 | 0.821699 | 3.51E-53 | -1.69E-46 |
| MS.gene049657 | MS.gene34383 | 0.809887 | 1.51E-50 | -1.69E-46 |
| MS.gene049865 | MS.gene34383 | 0.823453 | 1.37E-53 | -1.69E-46 |
| MS.gene05010 | MS.gene34383 | 0.844232 | 8.59E-59 | -1.69E-46 |
| MS.gene050170 | MS.gene34383 | 0.910406 | 1.94E-82 | -1.69E-46 |
| MS.gene050323 | MS.gene34383 | 0.881557 | 2.17E-70 | -1.69E-46 |
| MS.gene050338 | MS.gene34383 | -0.812803 | 3.53E-51 | -1.69E-46 |
| MS.gene050381 | MS.gene34383 | -0.805696 | 1.18E-49 | -1.69E-46 |
| MS.gene050496 | MS.gene34383 | 0.900486 | 6.98E-78 | -1.69E-46 |
| MS.gene050502 | MS.gene34383 | 0.881696 | 1.94E-70 | -1.69E-46 |
| MS.gene050504 | MS.gene34383 | 0.851437 | 8.88E-61 | -1.69E-46 |
| MS.gene050518 | MS.gene34383 | 0.829778 | 4.25E-55 | -1.69E-46 |
| MS.gene050538 | MS.gene34383 | 0.801825 | 7.50E-49 | -1.69E-46 |
| MS.gene050577 | MS.gene34383 | 0.883233 | 5.33E-71 | -1.69E-46 |
| MS.gene050612 | MS.gene34383 | 0.854458 | 1.21E-61 | -1.69E-46 |
| MS.gene050675 | MS.gene34383 | 0.837879 | 4.00E-57 | -1.69E-46 |
| MS.gene050697 | MS.gene34383 | 0.865511 | 5.60E-65 | -1.69E-46 |
| MS.gene050699 | MS.gene34383 | 0.847776 | 9.34E-60 | -1.69E-46 |
| MS.gene050747 | MS.gene34383 | 0.843681 | 1.21E-58 | -1.69E-46 |
| MS.gene050772 | MS.gene34383 | 0.839847 | 1.24E-57 | -1.69E-46 |
| MS.gene05080 | MS.gene34383 | 0.860301 | 2.27E-63 | -1.69E-46 |
| MS.gene05081 | MS.gene34383 | 0.809562 | 1.78E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene34383 | 0.877178 | 7.74E-69 | -1.69E-46 |
| MS.gene050871 | MS.gene34383 | 0.876493 | 1.34E-68 | -1.69E-46 |
| MS.gene050904 | MS.gene34383 | 0.847001 | 1.52E-59 | -1.69E-46 |
| MS.gene050950 | MS.gene34383 | 0.832976 | 6.95E-56 | -1.69E-46 |
| MS.gene050986 | MS.gene34383 | 0.845083 | 5.07E-59 | -1.69E-46 |
| MS.gene051268 | MS.gene34383 | 0.845232 | 4.62E-59 | -1.69E-46 |
| MS.gene051317 | MS.gene34383 | 0.824702 | 6.99E-54 | -1.69E-46 |
| MS.gene051509 | MS.gene34383 | 0.829757 | 4.31E-55 | -1.69E-46 |
| MS.gene051551 | MS.gene34383 | 0.824253 | 8.92E-54 | -1.69E-46 |
| MS.gene051651 | MS.gene34383 | -0.81126 | 7.65E-51 | -1.69E-46 |
| MS.gene051734 | MS.gene34383 | 0.811567 | 6.56E-51 | -1.69E-46 |
| MS.gene051838 | MS.gene34383 | 0.855018 | 8.36E-62 | -1.69E-46 |
| MS.gene051941 | MS.gene34383 | 0.833868 | 4.17E-56 | -1.69E-46 |
| MS.gene052066 | MS.gene34383 | 0.829453 | 5.10E-55 | -1.69E-46 |
| MS.gene052164 | MS.gene34383 | 0.891819 | 2.80E-74 | -1.69E-46 |
| MS.gene052346 | MS.gene34383 | 0.830119 | 3.51E-55 | -1.69E-46 |
| MS.gene052414 | MS.gene34383 | 0.81619 | 6.28E-52 | -1.69E-46 |
| MS.gene052482 | MS.gene34383 | 0.869295 | 3.45E-66 | -1.69E-46 |
| MS.gene052486 | MS.gene34383 | 0.875281 | 3.49E-68 | -1.69E-46 |
| MS.gene052494 | MS.gene34383 | -0.821938 | 3.09E-53 | -1.69E-46 |
| MS.gene052501 | MS.gene34383 | 0.815739 | 7.92E-52 | -1.69E-46 |
| MS.gene052503 | MS.gene34383 | 0.879187 | 1.53E-69 | -1.69E-46 |
| MS.gene052504 | MS.gene34383 | 0.821249 | 4.46E-53 | -1.69E-46 |
| MS.gene052506 | MS.gene34383 | 0.841599 | 4.31E-58 | -1.69E-46 |
| MS.gene052606 | MS.gene34383 | -0.803801 | 2.93E-49 | -1.69E-46 |
| MS.gene052822 | MS.gene34383 | 0.843626 | 1.25E-58 | -1.69E-46 |
| MS.gene052823 | MS.gene34383 | 0.882163 | 1.31E-70 | -1.69E-46 |
| MS.gene052825 | MS.gene34383 | 0.8736 | 1.30E-67 | -1.69E-46 |
| MS.gene052966 | MS.gene34383 | 0.864697 | 1.01E-64 | -1.69E-46 |
| MS.gene053097 | MS.gene34383 | -0.812746 | 3.63E-51 | -1.69E-46 |
| MS.gene053203 | MS.gene34383 | 0.867664 | 1.16E-65 | -1.69E-46 |
| MS.gene053213 | MS.gene34383 | 0.83254 | 8.92E-56 | -1.69E-46 |
| MS.gene053286 | MS.gene34383 | 0.836843 | 7.38E-57 | -1.69E-46 |
| MS.gene053420 | MS.gene34383 | -0.812278 | 4.59E-51 | -1.69E-46 |
| MS.gene053436 | MS.gene34383 | 0.867993 | 9.09E-66 | -1.69E-46 |
| MS.gene053479 | MS.gene34383 | 0.845071 | 5.10E-59 | -1.69E-46 |
| MS.gene053480 | MS.gene34383 | 0.827006 | 1.98E-54 | -1.69E-46 |
| MS.gene053574 | MS.gene34383 | 0.870677 | 1.22E-66 | -1.69E-46 |
| MS.gene053626 | MS.gene34383 | 0.836361 | 9.79E-57 | -1.69E-46 |
| MS.gene053633 | MS.gene34383 | 0.803895 | 2.80E-49 | -1.69E-46 |
| MS.gene053824 | MS.gene34383 | 0.855711 | 5.25E-62 | -1.69E-46 |
| MS.gene053916 | MS.gene34383 | 0.807666 | 4.52E-50 | -1.69E-46 |
| MS.gene054344 | MS.gene34383 | 0.824124 | 9.57E-54 | -1.69E-46 |
| MS.gene054408 | MS.gene34383 | 0.801827 | 7.49E-49 | -1.69E-46 |
| MS.gene054595 | MS.gene34383 | 0.841621 | 4.25E-58 | -1.69E-46 |
| MS.gene054600 | MS.gene34383 | 0.837326 | 5.55E-57 | -1.69E-46 |
| MS.gene054642 | MS.gene34383 | 0.814073 | 1.85E-51 | -1.69E-46 |
| MS.gene054706 | MS.gene34383 | -0.843378 | 1.45E-58 | -1.69E-46 |
| MS.gene05470 | MS.gene34383 | 0.879216 | 1.49E-69 | -1.69E-46 |
| MS.gene054927 | MS.gene34383 | 0.815553 | 8.71E-52 | -1.69E-46 |
| MS.gene054954 | MS.gene34383 | 0.854565 | 1.13E-61 | -1.69E-46 |
| MS.gene055224 | MS.gene34383 | 0.832826 | 7.57E-56 | -1.69E-46 |
| MS.gene055251 | MS.gene34383 | -0.804079 | 2.57E-49 | -1.69E-46 |
| MS.gene055362 | MS.gene34383 | 0.855248 | 7.17E-62 | -1.69E-46 |
| MS.gene055502 | MS.gene34383 | 0.849382 | 3.35E-60 | -1.69E-46 |
| MS.gene055577 | MS.gene34383 | 0.879272 | 1.43E-69 | -1.69E-46 |
| MS.gene055741 | MS.gene34383 | 0.81944 | 1.16E-52 | -1.69E-46 |
| MS.gene055744 | MS.gene34383 | 0.807914 | 4.00E-50 | -1.69E-46 |
| MS.gene055748 | MS.gene34383 | 0.869781 | 2.39E-66 | -1.69E-46 |
| MS.gene055750 | MS.gene34383 | 0.88418 | 2.39E-71 | -1.69E-46 |
| MS.gene055751 | MS.gene34383 | 0.874685 | 5.57E-68 | -1.69E-46 |
| MS.gene055776 | MS.gene34383 | 0.843854 | 1.08E-58 | -1.69E-46 |
| MS.gene05589 | MS.gene34383 | 0.922161 | 1.41E-88 | -1.69E-46 |
| MS.gene055941 | MS.gene34383 | 0.80998 | 1.45E-50 | -1.69E-46 |
| MS.gene056040 | MS.gene34383 | 0.818637 | 1.77E-52 | -1.69E-46 |
| MS.gene056046 | MS.gene34383 | 0.842716 | 2.18E-58 | -1.69E-46 |
| MS.gene056166 | MS.gene34383 | 0.845552 | 3.78E-59 | -1.69E-46 |
| MS.gene056266 | MS.gene34383 | 0.82567 | 4.13E-54 | -1.69E-46 |
| MS.gene056283 | MS.gene34383 | 0.827448 | 1.56E-54 | -1.69E-46 |
| MS.gene056387 | MS.gene34383 | 0.853184 | 2.83E-61 | -1.69E-46 |
| MS.gene056446 | MS.gene34383 | 0.835021 | 2.14E-56 | -1.69E-46 |
| MS.gene056504 | MS.gene34383 | 0.919658 | 3.42E-87 | -1.69E-46 |
| MS.gene056510 | MS.gene34383 | 0.848499 | 5.90E-60 | -1.69E-46 |
| MS.gene05664 | MS.gene34383 | 0.802091 | 6.61E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene34383 | 0.848636 | 5.40E-60 | -1.69E-46 |
| MS.gene05666 | MS.gene34383 | 0.812184 | 4.82E-51 | -1.69E-46 |
| MS.gene056692 | MS.gene34383 | 0.82849 | 8.74E-55 | -1.69E-46 |
| MS.gene056694 | MS.gene34383 | -0.81937 | 1.20E-52 | -1.69E-46 |
| MS.gene056877 | MS.gene34383 | 0.811774 | 5.92E-51 | -1.69E-46 |
| MS.gene056878 | MS.gene34383 | 0.820007 | 8.61E-53 | -1.69E-46 |
| MS.gene057019 | MS.gene34383 | 0.805937 | 1.05E-49 | -1.69E-46 |
| MS.gene057076 | MS.gene34383 | 0.817032 | 4.07E-52 | -1.69E-46 |
| MS.gene057086 | MS.gene34383 | 0.868499 | 6.24E-66 | -1.69E-46 |
| MS.gene057658 | MS.gene34383 | 0.843273 | 1.55E-58 | -1.69E-46 |
| MS.gene057687 | MS.gene34383 | 0.834288 | 3.27E-56 | -1.69E-46 |
| MS.gene057734 | MS.gene34383 | 0.829678 | 4.50E-55 | -1.69E-46 |
| MS.gene057831 | MS.gene34383 | 0.847999 | 8.11E-60 | -1.69E-46 |
| MS.gene05787 | MS.gene34383 | 0.842457 | 2.56E-58 | -1.69E-46 |
| MS.gene058157 | MS.gene34383 | 0.823733 | 1.18E-53 | -1.69E-46 |
| MS.gene058171 | MS.gene34383 | -0.802347 | 5.86E-49 | -1.69E-46 |
| MS.gene058175 | MS.gene34383 | -0.804408 | 2.19E-49 | -1.69E-46 |
| MS.gene058334 | MS.gene34383 | -0.870869 | 1.05E-66 | -1.69E-46 |
| MS.gene058338 | MS.gene34383 | 0.820176 | 7.88E-53 | -1.69E-46 |
| MS.gene05837 | MS.gene34383 | 0.852236 | 5.27E-61 | -1.69E-46 |
| MS.gene058382 | MS.gene34383 | -0.812956 | 3.26E-51 | -1.69E-46 |
| MS.gene058419 | MS.gene34383 | 0.848135 | 7.43E-60 | -1.69E-46 |
| MS.gene058544 | MS.gene34383 | 0.834803 | 2.43E-56 | -1.69E-46 |
| MS.gene058773 | MS.gene34383 | 0.817267 | 3.60E-52 | -1.69E-46 |
| MS.gene058838 | MS.gene34383 | 0.886248 | 4.03E-72 | -1.69E-46 |
| MS.gene059022 | MS.gene34383 | 0.843684 | 1.20E-58 | -1.69E-46 |
| MS.gene059023 | MS.gene34383 | 0.862774 | 3.99E-64 | -1.69E-46 |
| MS.gene05913 | MS.gene34383 | 0.828413 | 9.12E-55 | -1.69E-46 |
| MS.gene059367 | MS.gene34383 | 0.855139 | 7.71E-62 | -1.69E-46 |
| MS.gene059667 | MS.gene34383 | 0.905722 | 3.18E-80 | -1.69E-46 |
| MS.gene059677 | MS.gene34383 | 0.831769 | 1.38E-55 | -1.69E-46 |
| MS.gene059718 | MS.gene34383 | 0.841252 | 5.32E-58 | -1.69E-46 |
| MS.gene059756 | MS.gene34383 | 0.806193 | 9.27E-50 | -1.69E-46 |
| MS.gene059801 | MS.gene34383 | 0.826141 | 3.19E-54 | -1.69E-46 |
| MS.gene059837 | MS.gene34383 | 0.872935 | 2.17E-67 | -1.69E-46 |
| MS.gene059865 | MS.gene34383 | 0.89476 | 1.82E-75 | -1.69E-46 |
| MS.gene05995 | MS.gene34383 | 0.812399 | 4.32E-51 | -1.69E-46 |
| MS.gene05996 | MS.gene34383 | 0.82207 | 2.88E-53 | -1.69E-46 |
| MS.gene06000 | MS.gene34383 | 0.894773 | 1.80E-75 | -1.69E-46 |
| MS.gene06027 | MS.gene34383 | 0.837578 | 4.79E-57 | -1.69E-46 |
| MS.gene060482 | MS.gene34383 | 0.824039 | 1.00E-53 | -1.69E-46 |
| MS.gene060593 | MS.gene34383 | -0.837293 | 5.66E-57 | -1.69E-46 |
| MS.gene060595 | MS.gene34383 | -0.846065 | 2.74E-59 | -1.69E-46 |
| MS.gene060703 | MS.gene34383 | 0.852272 | 5.15E-61 | -1.69E-46 |
| MS.gene060762 | MS.gene34383 | 0.86121 | 1.20E-63 | -1.69E-46 |
| MS.gene060778 | MS.gene34383 | 0.879628 | 1.07E-69 | -1.69E-46 |
| MS.gene06077 | MS.gene34383 | 0.876726 | 1.11E-68 | -1.69E-46 |
| MS.gene060823 | MS.gene34383 | -0.803647 | 3.16E-49 | -1.69E-46 |
| MS.gene060835 | MS.gene34383 | 0.852312 | 5.01E-61 | -1.69E-46 |
| MS.gene06092 | MS.gene34383 | 0.811058 | 8.47E-51 | -1.69E-46 |
| MS.gene06097 | MS.gene34383 | 0.856769 | 2.57E-62 | -1.69E-46 |
| MS.gene060982 | MS.gene34383 | 0.924601 | 5.66E-90 | -1.69E-46 |
| MS.gene061044 | MS.gene34383 | -0.845718 | 3.41E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene006404 | MS.gene34383 | PPI |
| MS.gene34383 | MS.gene34385 | PPI |
| MS.gene34383 | MS.gene070957 | PPI |
| MS.gene37702 | MS.gene34383 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34383.t1 | MTR_1g102890 | 97.326 | 187 | 5 | 0 | 1 | 187 | 1 | 187 | 4.42e-136 | 378 |
| MS.gene34383.t1 | MTR_1g102890 | 94.631 | 149 | 8 | 0 | 1 | 149 | 1 | 149 | 1.98e-103 | 294 |
| MS.gene34383.t1 | MTR_1g102870 | 65.730 | 178 | 61 | 0 | 2 | 179 | 12 | 189 | 4.99e-91 | 265 |
| MS.gene34383.t1 | MTR_1g102870 | 67.586 | 145 | 47 | 0 | 2 | 146 | 12 | 156 | 5.86e-75 | 222 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34383.t1 | AT5G43850 | 78.610 | 187 | 40 | 0 | 1 | 187 | 1 | 187 | 1.89e-110 | 313 |
| MS.gene34383.t1 | AT2G26400 | 65.000 | 180 | 63 | 0 | 2 | 181 | 27 | 206 | 9.62e-90 | 262 |
| MS.gene34383.t1 | AT2G26400 | 65.000 | 180 | 63 | 0 | 2 | 181 | 12 | 191 | 1.24e-89 | 261 |
| MS.gene34383.t1 | AT4G14710 | 63.842 | 177 | 64 | 0 | 2 | 178 | 14 | 190 | 1.34e-89 | 261 |
| MS.gene34383.t1 | AT4G14710 | 63.842 | 177 | 64 | 0 | 2 | 178 | 12 | 188 | 1.62e-89 | 261 |
| MS.gene34383.t1 | AT4G14710 | 63.842 | 177 | 64 | 0 | 2 | 178 | 12 | 188 | 1.62e-89 | 261 |
| MS.gene34383.t1 | AT4G14716 | 64.535 | 172 | 61 | 0 | 2 | 173 | 12 | 183 | 4.76e-88 | 256 |
| MS.gene34383.t1 | AT4G14710 | 63.483 | 178 | 64 | 1 | 2 | 178 | 12 | 189 | 9.14e-88 | 256 |
| MS.gene34383.t1 | AT2G26400 | 67.262 | 168 | 55 | 0 | 2 | 169 | 27 | 194 | 2.91e-87 | 256 |
| MS.gene34383.t1 | AT2G26400 | 67.262 | 168 | 55 | 0 | 2 | 169 | 12 | 179 | 3.26e-87 | 255 |
| MS.gene34383.t1 | AT4G14710 | 64.881 | 168 | 59 | 0 | 2 | 169 | 12 | 179 | 1.22e-86 | 253 |
| MS.gene34383.t1 | AT4G14710 | 64.327 | 171 | 61 | 0 | 8 | 178 | 1 | 171 | 2.87e-86 | 252 |
| MS.gene34383.t1 | AT4G14716 | 65.060 | 166 | 58 | 0 | 8 | 173 | 1 | 166 | 1.07e-84 | 248 |
Find 48 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTAGAAAGGAATACATTA+AGG | 0.274694 | 1.2:-76580569 | MS.gene34383:CDS |
| TGATCATTCTCATAGTCATT+AGG | 0.279982 | 1.2:+76581800 | None:intergenic |
| ATGATTAATAACCTGCTAAT+TGG | 0.292079 | 1.2:+76581922 | None:intergenic |
| GATCATTCTCATAGTCATTA+GGG | 0.297534 | 1.2:+76581801 | None:intergenic |
| GCAGTTAATGAGGTTGTTTA+TGG | 0.306900 | 1.2:-76580638 | MS.gene34383:intron |
| CCCCAATGAATTTGTTTCCT+TGG | 0.318267 | 1.2:-76581946 | MS.gene34383:CDS |
| CAGAATTGGGAGTCTTATAC+TGG | 0.335864 | 1.2:-76581832 | MS.gene34383:CDS |
| GTCCAAGGAAACAAATTCAT+TGG | 0.347689 | 1.2:+76581944 | None:intergenic |
| TGAAGAGATTCGTTACTGTT+TGG | 0.363134 | 1.2:-76580972 | MS.gene34383:CDS |
| CTTCGATGTTCGAGACAAAA+AGG | 0.370547 | 1.2:-76580936 | MS.gene34383:CDS |
| TGTGCCACTTGCAGCTCATT+AGG | 0.372112 | 1.2:-76580527 | None:intergenic |
| AGGATCGCTGGATTCGCATT+TGG | 0.387790 | 1.2:-76580916 | MS.gene34383:CDS |
| TCCAAGGAAACAAATTCATT+GGG | 0.393021 | 1.2:+76581945 | None:intergenic |
| TACCATGTAATTGTATCCTC+TGG | 0.413679 | 1.2:+76581754 | None:intergenic |
| TTAATGTATTCCTTTCTAGC+AGG | 0.416178 | 1.2:+76580571 | None:intergenic |
| CTGGATTCGCATTTGGATCA+AGG | 0.420712 | 1.2:-76580909 | MS.gene34383:CDS |
| CAGTTAATGAGGTTGTTTAT+GGG | 0.429331 | 1.2:-76580637 | MS.gene34383:CDS |
| GCTAGAAAGGAATACATTAA+GGG | 0.444455 | 1.2:-76580568 | MS.gene34383:CDS |
| GGAAGATAACCCTGCTAGAA+AGG | 0.448092 | 1.2:-76580581 | MS.gene34383:CDS |
| ATTGTGATAGGCATGGTTCA+TGG | 0.474686 | 1.2:-76582009 | MS.gene34383:intron |
| GTTTCCTTGGACCAATTAGC+AGG | 0.487915 | 1.2:-76581933 | MS.gene34383:intron |
| CTACACTGAGCACATTCATC+AGG | 0.491343 | 1.2:-76580996 | MS.gene34383:CDS |
| CGTTACTGTTTGGAGGGAAG+TGG | 0.493451 | 1.2:-76580962 | MS.gene34383:CDS |
| AAGGGCTTGACTGAGAAAAC+TGG | 0.495479 | 1.2:-76580550 | MS.gene34383:CDS |
| TAGCCATGGTGAGGAAGTCT+TGG | 0.507430 | 1.2:+76581969 | None:intergenic |
| AGAGATTCGTTACTGTTTGG+AGG | 0.508489 | 1.2:-76580969 | MS.gene34383:CDS |
| TAATGTATTCCTTTCTAGCA+GGG | 0.526328 | 1.2:+76580572 | None:intergenic |
| ATTCGCATTTGGATCAAGGC+TGG | 0.542481 | 1.2:-76580905 | MS.gene34383:CDS |
| AACCGATGGTAGATTCCTGC+AGG | 0.545163 | 1.2:+76580866 | None:intergenic |
| GGATTTACTTGATTTATGCC+CGG | 0.551505 | 1.2:-76581056 | MS.gene34383:CDS |
| CAAATTCATTGGGGTAGCCA+TGG | 0.553222 | 1.2:+76581955 | None:intergenic |
| GAGATTCGTTACTGTTTGGA+GGG | 0.561571 | 1.2:-76580968 | MS.gene34383:CDS |
| GAGGTTGTTTATGGGTGAGC+CGG | 0.570174 | 1.2:-76580629 | MS.gene34383:CDS |
| ATTGTTACTCATGGCGATTG+AGG | 0.572796 | 1.2:-76582098 | None:intergenic |
| CTAGCAGGGTTATCTTCCTG+TGG | 0.575444 | 1.2:+76580586 | None:intergenic |
| TACCTGCAGGAATCTACCAT+CGG | 0.576810 | 1.2:-76580868 | MS.gene34383:CDS |
| CGATTATATGCTGTCCACAC+CGG | 0.591841 | 1.2:+76580610 | None:intergenic |
| CGACACAAATAACTATGTGA+AGG | 0.598188 | 1.2:-76580837 | MS.gene34383:intron |
| ATAACCTGCTAATTGGTCCA+AGG | 0.599536 | 1.2:+76581929 | None:intergenic |
| TTGTGTCGAGAGTGAACCGA+TGG | 0.605251 | 1.2:+76580852 | None:intergenic |
| TGTTTATGGGTGAGCCGGTG+TGG | 0.615047 | 1.2:-76580624 | MS.gene34383:CDS |
| AGCCAGAGGATACAATTACA+TGG | 0.637470 | 1.2:-76581756 | MS.gene34383:intron |
| GACAGCATATAATCGACCAC+AGG | 0.646228 | 1.2:-76580602 | MS.gene34383:CDS |
| GGGTCCTAATGAGCTGCAAG+TGG | 0.648299 | 1.2:+76580523 | None:intergenic |
| TCATTGGGGTAGCCATGGTG+AGG | 0.672207 | 1.2:+76581960 | None:intergenic |
| GATCCAAGACTTCCTCACCA+TGG | 0.679886 | 1.2:-76581972 | MS.gene34383:CDS |
| AAGAACATTAGAGAAGCCAG+AGG | 0.683987 | 1.2:-76581770 | MS.gene34383:CDS |
| CCAAGGAAACAAATTCATTG+GGG | 0.686962 | 1.2:+76581946 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATCTAATATATTGTAAA+CGG | - | chr1.2:76581070-76581089 | MS.gene34383:intron | 10.0% |
| !! | TAATAATTAAAAATTGTGAT+AGG | - | chr1.2:76580595-76580614 | MS.gene34383:CDS | 10.0% |
| !! | TATATTAAGATTAATTCATA+AGG | + | chr1.2:76580562-76580581 | None:intergenic | 10.0% |
| !! | AATTAGTTGAAAATAACTTA+CGG | - | chr1.2:76581284-76581303 | MS.gene34383:intron | 15.0% |
| !! | TAAAAAAAACATGTTAAAAC+AGG | - | chr1.2:76581032-76581051 | MS.gene34383:CDS | 15.0% |
| !!! | AATTTTAATTTTTGCAGAAT+TGG | - | chr1.2:76580770-76580789 | MS.gene34383:intron | 15.0% |
| !!! | ATTTTAATTTTTGCAGAATT+GGG | - | chr1.2:76580771-76580790 | MS.gene34383:intron | 15.0% |
| !! | TTACATCAACAAAAATTTGA+AGG | + | chr1.2:76581096-76581115 | None:intergenic | 20.0% |
| !! | TTTGTTGATGTAAATTTCTT+AGG | - | chr1.2:76581103-76581122 | MS.gene34383:intron | 20.0% |
| !!! | ATTTTGATTGTACTAATTGA+AGG | - | chr1.2:76581456-76581475 | MS.gene34383:intron | 20.0% |
| !!! | TAACATGTTTTTTTTACATG+TGG | + | chr1.2:76581003-76581022 | None:intergenic | 20.0% |
| !!! | TCATAATTTTCAACTTTTTC+CGG | + | chr1.2:76581582-76581601 | None:intergenic | 20.0% |
| !!! | TTTTGATTGTACTAATTGAA+GGG | - | chr1.2:76581457-76581476 | MS.gene34383:intron | 20.0% |
| ! | ATAGCTTATGAAAACAACTT+TGG | + | chr1.2:76581315-76581334 | None:intergenic | 25.0% |
| ! | ATGATTAATAACCTGCTAAT+TGG | + | chr1.2:76580697-76580716 | None:intergenic | 25.0% |
| ! | ATTAAAAATTGTGATAGGCA+TGG | - | chr1.2:76580600-76580619 | MS.gene34383:CDS | 25.0% |
| ! | ATTTCATCTTTCAAATGTTC+TGG | - | chr1.2:76581482-76581501 | MS.gene34383:intron | 25.0% |
| ! | GTACTATTTGAATATGTTGT+AGG | - | chr1.2:76581539-76581558 | MS.gene34383:intron | 25.0% |
| ! | TTGAAATTGATCAAGATTCA+AGG | - | chr1.2:76581896-76581915 | MS.gene34383:intron | 25.0% |
| ! | TTTCATCTTTCAAATGTTCT+GGG | - | chr1.2:76581483-76581502 | MS.gene34383:intron | 25.0% |
| !! | AAGTTATCTTTGAGAGTTTA+TGG | - | chr1.2:76581260-76581279 | MS.gene34383:intron | 25.0% |
| !! | TTTCTAGTGATACAATTACA+CGG | - | chr1.2:76580945-76580964 | MS.gene34383:CDS | 25.0% |
| !!! | CATAATTTTCAACTTTTTCC+GGG | + | chr1.2:76581581-76581600 | None:intergenic | 25.0% |
| ATAAGTTGTTTACCTAACAG+TGG | + | chr1.2:76581157-76581176 | None:intergenic | 30.0% | |
| CAAGATTCAAGGTAATATGT+TGG | - | chr1.2:76581907-76581926 | MS.gene34383:intron | 30.0% | |
| CAGTTAATGAGGTTGTTTAT+GGG | - | chr1.2:76581979-76581998 | MS.gene34383:CDS | 30.0% | |
| CCTATCAATATTCTTGATCA+AGG | - | chr1.2:76581810-76581829 | MS.gene34383:CDS | 30.0% | |
| CCTTGATCAAGAATATTGAT+AGG | + | chr1.2:76581813-76581832 | None:intergenic | 30.0% | |
| GAGCTCAAATAAGTCAATTT+AGG | - | chr1.2:76581370-76581389 | MS.gene34383:intron | 30.0% | |
| GATCATTCTCATAGTCATTA+GGG | + | chr1.2:76580818-76580837 | None:intergenic | 30.0% | |
| GCTAGAAAGGAATACATTAA+GGG | - | chr1.2:76582048-76582067 | MS.gene34383:intron | 30.0% | |
| GTACAATCAAAATGTTCAAC+AGG | + | chr1.2:76581449-76581468 | None:intergenic | 30.0% | |
| TCCAAGGAAACAAATTCATT+GGG | + | chr1.2:76580674-76580693 | None:intergenic | 30.0% | |
| TGATCATTCTCATAGTCATT+AGG | + | chr1.2:76580819-76580838 | None:intergenic | 30.0% | |
| TGCTAGAAAGGAATACATTA+AGG | - | chr1.2:76582047-76582066 | MS.gene34383:intron | 30.0% | |
| TTATTGTGACACAAGTTTGA+GGG | - | chr1.2:76580745-76580764 | MS.gene34383:intron | 30.0% | |
| TTTATTGTGACACAAGTTTG+AGG | - | chr1.2:76580744-76580763 | MS.gene34383:intron | 30.0% | |
| ! | TAATGTATTCCTTTCTAGCA+GGG | + | chr1.2:76582047-76582066 | None:intergenic | 30.0% |
| ! | TTAATGTATTCCTTTCTAGC+AGG | + | chr1.2:76582048-76582067 | None:intergenic | 30.0% |
| !! | TTGATCAAGGCTTCATTTTT+GGG | - | chr1.2:76581823-76581842 | MS.gene34383:CDS | 30.0% |
| !!! | TGAAAGTGGATTTTTTCTTG+CGG | - | chr1.2:76580974-76580993 | MS.gene34383:CDS | 30.0% |
| ACTTATTTGAGCTCATCTAC+CGG | + | chr1.2:76581364-76581383 | None:intergenic | 35.0% | |
| CCAAGGAAACAAATTCATTG+GGG | + | chr1.2:76580673-76580692 | None:intergenic | 35.0% | |
| CGACACAAATAACTATGTGA+AGG | - | chr1.2:76581779-76581798 | MS.gene34383:CDS | 35.0% | |
| GCAGTTAATGAGGTTGTTTA+TGG | - | chr1.2:76581978-76581997 | MS.gene34383:CDS | 35.0% | |
| GGATTTACTTGATTTATGCC+CGG | - | chr1.2:76581560-76581579 | MS.gene34383:intron | 35.0% | |
| GTCCAAGGAAACAAATTCAT+TGG | + | chr1.2:76580675-76580694 | None:intergenic | 35.0% | |
| TACCATGTAATTGTATCCTC+TGG | + | chr1.2:76580865-76580884 | None:intergenic | 35.0% | |
| TGAAGAGATTCGTTACTGTT+TGG | - | chr1.2:76581644-76581663 | MS.gene34383:intron | 35.0% | |
| TTACACGGTAAGATTGAAAG+TGG | - | chr1.2:76580960-76580979 | MS.gene34383:CDS | 35.0% | |
| TTGTTCGATGCATATGTATG+TGG | - | chr1.2:76581949-76581968 | MS.gene34383:CDS | 35.0% | |
| ! | CTTGATCAAGGCTTCATTTT+TGG | - | chr1.2:76581822-76581841 | MS.gene34383:CDS | 35.0% |
| ! | GATCTTATCATTTTACCTGC+AGG | - | chr1.2:76581735-76581754 | MS.gene34383:intron | 35.0% |
| !!! | GAAAGTGGATTTTTTCTTGC+GGG | - | chr1.2:76580975-76580994 | MS.gene34383:CDS | 35.0% |
| AAGAACATTAGAGAAGCCAG+AGG | - | chr1.2:76580846-76580865 | MS.gene34383:CDS | 40.0% | |
| AGAGATTCGTTACTGTTTGG+AGG | - | chr1.2:76581647-76581666 | MS.gene34383:intron | 40.0% | |
| AGCCAGAGGATACAATTACA+TGG | - | chr1.2:76580860-76580879 | MS.gene34383:CDS | 40.0% | |
| AGTCTCGCAAATACTTATGC+CGG | - | chr1.2:76581342-76581361 | MS.gene34383:intron | 40.0% | |
| ATAACCTGCTAATTGGTCCA+AGG | + | chr1.2:76580690-76580709 | None:intergenic | 40.0% | |
| ATTGTGATAGGCATGGTTCA+TGG | - | chr1.2:76580607-76580626 | MS.gene34383:CDS | 40.0% | |
| CTTCGATGTTCGAGACAAAA+AGG | - | chr1.2:76581680-76581699 | MS.gene34383:intron | 40.0% | |
| GAGATTCGTTACTGTTTGGA+GGG | - | chr1.2:76581648-76581667 | MS.gene34383:intron | 40.0% | |
| ! | CAGAATTGGGAGTCTTATAC+TGG | - | chr1.2:76580784-76580803 | MS.gene34383:intron | 40.0% |
| ! | CCCCAATGAATTTGTTTCCT+TGG | - | chr1.2:76580670-76580689 | MS.gene34383:intron | 40.0% |
| !!! | AAGGCTTCATTTTTGGGATG+TGG | - | chr1.2:76581829-76581848 | MS.gene34383:CDS | 40.0% |
| !!! | GTGGTTTTGTGCAGTTAATG+AGG | - | chr1.2:76581968-76581987 | MS.gene34383:CDS | 40.0% |
| AAGGGCTTGACTGAGAAAAC+TGG | - | chr1.2:76582066-76582085 | MS.gene34383:intron | 45.0% | |
| ATTCGCATTTGGATCAAGGC+TGG | - | chr1.2:76581711-76581730 | MS.gene34383:intron | 45.0% | |
| CAAATTCATTGGGGTAGCCA+TGG | + | chr1.2:76580664-76580683 | None:intergenic | 45.0% | |
| CGATTATATGCTGTCCACAC+CGG | + | chr1.2:76582009-76582028 | None:intergenic | 45.0% | |
| CTACACTGAGCACATTCATC+AGG | - | chr1.2:76581620-76581639 | MS.gene34383:intron | 45.0% | |
| CTGGATTCGCATTTGGATCA+AGG | - | chr1.2:76581707-76581726 | MS.gene34383:intron | 45.0% | |
| GACAGCATATAATCGACCAC+AGG | - | chr1.2:76582014-76582033 | MS.gene34383:intron | 45.0% | |
| GGAAGATAACCCTGCTAGAA+AGG | - | chr1.2:76582035-76582054 | MS.gene34383:intron | 45.0% | |
| GTTTCCTTGGACCAATTAGC+AGG | - | chr1.2:76580683-76580702 | MS.gene34383:intron | 45.0% | |
| TACCTGCAGGAATCTACCAT+CGG | - | chr1.2:76581748-76581767 | MS.gene34383:intron | 45.0% | |
| TTCGAGACAAAAAGGATCGC+TGG | - | chr1.2:76581688-76581707 | MS.gene34383:intron | 45.0% | |
| AACCGATGGTAGATTCCTGC+AGG | + | chr1.2:76581753-76581772 | None:intergenic | 50.0% | |
| AGGATCGCTGGATTCGCATT+TGG | - | chr1.2:76581700-76581719 | MS.gene34383:intron | 50.0% | |
| CGTTACTGTTTGGAGGGAAG+TGG | - | chr1.2:76581654-76581673 | MS.gene34383:intron | 50.0% | |
| CTAGCAGGGTTATCTTCCTG+TGG | + | chr1.2:76582033-76582052 | None:intergenic | 50.0% | |
| GAGGTTGTTTATGGGTGAGC+CGG | - | chr1.2:76581987-76582006 | MS.gene34383:CDS | 50.0% | |
| GATCCAAGACTTCCTCACCA+TGG | - | chr1.2:76580644-76580663 | MS.gene34383:intron | 50.0% | |
| TAGCCATGGTGAGGAAGTCT+TGG | + | chr1.2:76580650-76580669 | None:intergenic | 50.0% | |
| TTAGCAGCAACGCCACTGTT+AGG | - | chr1.2:76581142-76581161 | MS.gene34383:intron | 50.0% | |
| TTGTGTCGAGAGTGAACCGA+TGG | + | chr1.2:76581767-76581786 | None:intergenic | 50.0% | |
| TCATTGGGGTAGCCATGGTG+AGG | + | chr1.2:76580659-76580678 | None:intergenic | 55.0% | |
| TGTTTATGGGTGAGCCGGTG+TGG | - | chr1.2:76581992-76582011 | MS.gene34383:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 76580528 | 76582110 | 76580528 | ID=MS.gene34383 |
| chr1.2 | mRNA | 76580528 | 76582110 | 76580528 | ID=MS.gene34383.t1;Parent=MS.gene34383 |
| chr1.2 | exon | 76582099 | 76582110 | 76582099 | ID=MS.gene34383.t1.exon1;Parent=MS.gene34383.t1 |
| chr1.2 | CDS | 76582099 | 76582110 | 76582099 | ID=cds.MS.gene34383.t1;Parent=MS.gene34383.t1 |
| chr1.2 | exon | 76581934 | 76582021 | 76581934 | ID=MS.gene34383.t1.exon2;Parent=MS.gene34383.t1 |
| chr1.2 | CDS | 76581934 | 76582021 | 76581934 | ID=cds.MS.gene34383.t1;Parent=MS.gene34383.t1 |
| chr1.2 | exon | 76581757 | 76581851 | 76581757 | ID=MS.gene34383.t1.exon3;Parent=MS.gene34383.t1 |
| chr1.2 | CDS | 76581757 | 76581851 | 76581757 | ID=cds.MS.gene34383.t1;Parent=MS.gene34383.t1 |
| chr1.2 | exon | 76580838 | 76581077 | 76580838 | ID=MS.gene34383.t1.exon4;Parent=MS.gene34383.t1 |
| chr1.2 | CDS | 76580838 | 76581077 | 76580838 | ID=cds.MS.gene34383.t1;Parent=MS.gene34383.t1 |
| chr1.2 | exon | 76580528 | 76580656 | 76580528 | ID=MS.gene34383.t1.exon5;Parent=MS.gene34383.t1 |
| chr1.2 | CDS | 76580528 | 76580656 | 76580528 | ID=cds.MS.gene34383.t1;Parent=MS.gene34383.t1 |
| Gene Sequence |
| Protein sequence |
