Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53579.t1 | XP_024630994.1 | 96.7 | 61 | 2 | 0 | 1 | 61 | 20 | 80 | 1.10E-22 | 115.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53579.t1 | Q9T0H2 | 59.7 | 62 | 24 | 1 | 1 | 61 | 1 | 62 | 1.8e-10 | 65.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53579.t1 | A0A1S2XYN4 | 73.8 | 61 | 15 | 1 | 1 | 61 | 1 | 60 | 7.9e-15 | 88.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene53579 | 0.816751 | 4.71E-52 | -1.69E-46 |
| MS.gene049159 | MS.gene53579 | 0.857956 | 1.15E-62 | -1.69E-46 |
| MS.gene049254 | MS.gene53579 | 0.844053 | 9.59E-59 | -1.69E-46 |
| MS.gene049255 | MS.gene53579 | 0.832846 | 7.49E-56 | -1.69E-46 |
| MS.gene049404 | MS.gene53579 | 0.804764 | 1.85E-49 | -1.69E-46 |
| MS.gene049435 | MS.gene53579 | 0.827369 | 1.63E-54 | -1.69E-46 |
| MS.gene049452 | MS.gene53579 | 0.812028 | 5.21E-51 | -1.69E-46 |
| MS.gene049501 | MS.gene53579 | 0.8194 | 1.18E-52 | -1.69E-46 |
| MS.gene049756 | MS.gene53579 | 0.807039 | 6.14E-50 | -1.69E-46 |
| MS.gene049821 | MS.gene53579 | 0.838619 | 2.58E-57 | -1.69E-46 |
| MS.gene049850 | MS.gene53579 | 0.825121 | 5.57E-54 | -1.69E-46 |
| MS.gene049983 | MS.gene53579 | 0.801471 | 8.86E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene53579 | 0.822482 | 2.31E-53 | -1.69E-46 |
| MS.gene050277 | MS.gene53579 | 0.801095 | 1.06E-48 | -1.69E-46 |
| MS.gene050303 | MS.gene53579 | 0.845193 | 4.73E-59 | -1.69E-46 |
| MS.gene050355 | MS.gene53579 | 0.819694 | 1.02E-52 | -1.69E-46 |
| MS.gene050442 | MS.gene53579 | 0.851351 | 9.39E-61 | -1.69E-46 |
| MS.gene050559 | MS.gene53579 | 0.807611 | 4.64E-50 | -1.69E-46 |
| MS.gene050561 | MS.gene53579 | 0.810387 | 1.18E-50 | -1.69E-46 |
| MS.gene050605 | MS.gene53579 | 0.828994 | 6.60E-55 | -1.69E-46 |
| MS.gene050680 | MS.gene53579 | 0.806487 | 8.03E-50 | -1.69E-46 |
| MS.gene050689 | MS.gene53579 | 0.805351 | 1.39E-49 | -1.69E-46 |
| MS.gene050719 | MS.gene53579 | 0.810339 | 1.21E-50 | -1.69E-46 |
| MS.gene050721 | MS.gene53579 | 0.80838 | 3.19E-50 | -1.69E-46 |
| MS.gene050724 | MS.gene53579 | 0.806851 | 6.73E-50 | -1.69E-46 |
| MS.gene050750 | MS.gene53579 | 0.833952 | 3.97E-56 | -1.69E-46 |
| MS.gene050759 | MS.gene53579 | 0.812519 | 4.07E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene53579 | 0.825304 | 5.04E-54 | -1.69E-46 |
| MS.gene050815 | MS.gene53579 | 0.83389 | 4.11E-56 | -1.69E-46 |
| MS.gene050844 | MS.gene53579 | 0.816077 | 6.66E-52 | -1.69E-46 |
| MS.gene050953 | MS.gene53579 | 0.8454 | 4.16E-59 | -1.69E-46 |
| MS.gene050962 | MS.gene53579 | 0.806241 | 9.05E-50 | -1.69E-46 |
| MS.gene051088 | MS.gene53579 | 0.802962 | 4.37E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene53579 | 0.826463 | 2.68E-54 | -1.69E-46 |
| MS.gene051243 | MS.gene53579 | 0.844017 | 9.81E-59 | -1.69E-46 |
| MS.gene051247 | MS.gene53579 | 0.820603 | 6.29E-53 | -1.69E-46 |
| MS.gene051328 | MS.gene53579 | 0.837317 | 5.58E-57 | -1.69E-46 |
| MS.gene051344 | MS.gene53579 | 0.811071 | 8.41E-51 | -1.69E-46 |
| MS.gene05134 | MS.gene53579 | 0.830676 | 2.57E-55 | -1.69E-46 |
| MS.gene051363 | MS.gene53579 | 0.814496 | 1.50E-51 | -1.69E-46 |
| MS.gene051452 | MS.gene53579 | 0.818706 | 1.70E-52 | -1.69E-46 |
| MS.gene051744 | MS.gene53579 | 0.827579 | 1.45E-54 | -1.69E-46 |
| MS.gene05188 | MS.gene53579 | 0.835931 | 1.26E-56 | -1.69E-46 |
| MS.gene051975 | MS.gene53579 | 0.840147 | 1.04E-57 | -1.69E-46 |
| MS.gene052012 | MS.gene53579 | 0.83572 | 1.42E-56 | -1.69E-46 |
| MS.gene052020 | MS.gene53579 | 0.803614 | 3.21E-49 | -1.69E-46 |
| MS.gene052286 | MS.gene53579 | 0.806951 | 6.41E-50 | -1.69E-46 |
| MS.gene052376 | MS.gene53579 | 0.803923 | 2.76E-49 | -1.69E-46 |
| MS.gene052426 | MS.gene53579 | 0.827849 | 1.25E-54 | -1.69E-46 |
| MS.gene052516 | MS.gene53579 | 0.803384 | 3.58E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene53579 | 0.829103 | 6.21E-55 | -1.69E-46 |
| MS.gene052582 | MS.gene53579 | 0.830123 | 3.51E-55 | -1.69E-46 |
| MS.gene052584 | MS.gene53579 | 0.837006 | 6.71E-57 | -1.69E-46 |
| MS.gene052773 | MS.gene53579 | 0.816584 | 5.13E-52 | -1.69E-46 |
| MS.gene052863 | MS.gene53579 | 0.819363 | 1.21E-52 | -1.69E-46 |
| MS.gene052867 | MS.gene53579 | 0.808999 | 2.35E-50 | -1.69E-46 |
| MS.gene052896 | MS.gene53579 | 0.800203 | 1.61E-48 | -1.69E-46 |
| MS.gene052947 | MS.gene53579 | 0.80106 | 1.08E-48 | -1.69E-46 |
| MS.gene052954 | MS.gene53579 | 0.824183 | 9.27E-54 | -1.69E-46 |
| MS.gene053006 | MS.gene53579 | 0.840366 | 9.08E-58 | -1.69E-46 |
| MS.gene053008 | MS.gene53579 | 0.872533 | 2.96E-67 | -1.69E-46 |
| MS.gene053074 | MS.gene53579 | 0.804461 | 2.14E-49 | -1.69E-46 |
| MS.gene053080 | MS.gene53579 | 0.81182 | 5.78E-51 | -1.69E-46 |
| MS.gene053166 | MS.gene53579 | 0.813971 | 1.95E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene53579 | 0.849599 | 2.92E-60 | -1.69E-46 |
| MS.gene05364 | MS.gene53579 | 0.834941 | 2.24E-56 | -1.69E-46 |
| MS.gene053931 | MS.gene53579 | 0.842933 | 1.91E-58 | -1.69E-46 |
| MS.gene05414 | MS.gene53579 | 0.81289 | 3.38E-51 | -1.69E-46 |
| MS.gene05415 | MS.gene53579 | 0.851287 | 9.79E-61 | -1.69E-46 |
| MS.gene054236 | MS.gene53579 | 0.812568 | 3.97E-51 | -1.69E-46 |
| MS.gene054405 | MS.gene53579 | 0.838483 | 2.80E-57 | -1.69E-46 |
| MS.gene054472 | MS.gene53579 | 0.81635 | 5.79E-52 | -1.69E-46 |
| MS.gene054559 | MS.gene53579 | 0.801111 | 1.05E-48 | -1.69E-46 |
| MS.gene054651 | MS.gene53579 | 0.825738 | 3.98E-54 | -1.69E-46 |
| MS.gene054657 | MS.gene53579 | 0.876292 | 1.57E-68 | -1.69E-46 |
| MS.gene054658 | MS.gene53579 | 0.840154 | 1.03E-57 | -1.69E-46 |
| MS.gene05476 | MS.gene53579 | 0.809189 | 2.14E-50 | -1.69E-46 |
| MS.gene054775 | MS.gene53579 | 0.820044 | 8.45E-53 | -1.69E-46 |
| MS.gene054994 | MS.gene53579 | 0.824548 | 7.60E-54 | -1.69E-46 |
| MS.gene05510 | MS.gene53579 | 0.851792 | 7.05E-61 | -1.69E-46 |
| MS.gene055166 | MS.gene53579 | 0.821254 | 4.45E-53 | -1.69E-46 |
| MS.gene05517 | MS.gene53579 | 0.849226 | 3.70E-60 | -1.69E-46 |
| MS.gene055222 | MS.gene53579 | 0.835956 | 1.24E-56 | -1.69E-46 |
| MS.gene055227 | MS.gene53579 | 0.805042 | 1.62E-49 | -1.69E-46 |
| MS.gene055228 | MS.gene53579 | 0.810542 | 1.09E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene53579 | 0.809964 | 1.46E-50 | -1.69E-46 |
| MS.gene055512 | MS.gene53579 | 0.822281 | 2.58E-53 | -1.69E-46 |
| MS.gene055589 | MS.gene53579 | 0.800811 | 1.21E-48 | -1.69E-46 |
| MS.gene055608 | MS.gene53579 | 0.818468 | 1.93E-52 | -1.69E-46 |
| MS.gene055623 | MS.gene53579 | 0.865979 | 3.98E-65 | -1.69E-46 |
| MS.gene055701 | MS.gene53579 | 0.811307 | 7.47E-51 | -1.69E-46 |
| MS.gene055778 | MS.gene53579 | 0.830096 | 3.56E-55 | -1.69E-46 |
| MS.gene055808 | MS.gene53579 | 0.830285 | 3.20E-55 | -1.69E-46 |
| MS.gene055818 | MS.gene53579 | 0.847132 | 1.40E-59 | -1.69E-46 |
| MS.gene055841 | MS.gene53579 | 0.842006 | 3.37E-58 | -1.69E-46 |
| MS.gene055926 | MS.gene53579 | 0.826222 | 3.05E-54 | -1.69E-46 |
| MS.gene05604 | MS.gene53579 | 0.813248 | 2.82E-51 | -1.69E-46 |
| MS.gene056162 | MS.gene53579 | 0.828712 | 7.72E-55 | -1.69E-46 |
| MS.gene056173 | MS.gene53579 | 0.801417 | 9.09E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene53579 | 0.826899 | 2.11E-54 | -1.69E-46 |
| MS.gene056215 | MS.gene53579 | 0.862396 | 5.22E-64 | -1.69E-46 |
| MS.gene056366 | MS.gene53579 | 0.806315 | 8.73E-50 | -1.69E-46 |
| MS.gene056498 | MS.gene53579 | 0.800651 | 1.30E-48 | -1.69E-46 |
| MS.gene056506 | MS.gene53579 | 0.827323 | 1.67E-54 | -1.69E-46 |
| MS.gene056595 | MS.gene53579 | 0.825172 | 5.42E-54 | -1.69E-46 |
| MS.gene056631 | MS.gene53579 | 0.820507 | 6.61E-53 | -1.69E-46 |
| MS.gene056760 | MS.gene53579 | 0.814497 | 1.49E-51 | -1.69E-46 |
| MS.gene056776 | MS.gene53579 | 0.817484 | 3.22E-52 | -1.69E-46 |
| MS.gene05690 | MS.gene53579 | 0.806068 | 9.84E-50 | -1.69E-46 |
| MS.gene056986 | MS.gene53579 | 0.822553 | 2.23E-53 | -1.69E-46 |
| MS.gene057060 | MS.gene53579 | 0.858538 | 7.69E-63 | -1.69E-46 |
| MS.gene057079 | MS.gene53579 | 0.802592 | 5.21E-49 | -1.69E-46 |
| MS.gene057140 | MS.gene53579 | 0.819901 | 9.10E-53 | -1.69E-46 |
| MS.gene057208 | MS.gene53579 | 0.849973 | 2.29E-60 | -1.69E-46 |
| MS.gene057314 | MS.gene53579 | 0.841547 | 4.45E-58 | -1.69E-46 |
| MS.gene057444 | MS.gene53579 | 0.819363 | 1.21E-52 | -1.69E-46 |
| MS.gene057598 | MS.gene53579 | 0.818165 | 2.26E-52 | -1.69E-46 |
| MS.gene057693 | MS.gene53579 | 0.886054 | 4.78E-72 | -1.69E-46 |
| MS.gene057705 | MS.gene53579 | 0.804483 | 2.11E-49 | -1.69E-46 |
| MS.gene057855 | MS.gene53579 | 0.805309 | 1.42E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene53579 | 0.827656 | 1.39E-54 | -1.69E-46 |
| MS.gene057915 | MS.gene53579 | 0.825928 | 3.59E-54 | -1.69E-46 |
| MS.gene05795 | MS.gene53579 | 0.817413 | 3.34E-52 | -1.69E-46 |
| MS.gene058009 | MS.gene53579 | 0.817484 | 3.22E-52 | -1.69E-46 |
| MS.gene058010 | MS.gene53579 | 0.824185 | 9.25E-54 | -1.69E-46 |
| MS.gene058037 | MS.gene53579 | 0.805184 | 1.51E-49 | -1.69E-46 |
| MS.gene058084 | MS.gene53579 | 0.819493 | 1.13E-52 | -1.69E-46 |
| MS.gene058126 | MS.gene53579 | 0.821114 | 4.79E-53 | -1.69E-46 |
| MS.gene058146 | MS.gene53579 | 0.841255 | 5.31E-58 | -1.69E-46 |
| MS.gene058292 | MS.gene53579 | 0.805835 | 1.10E-49 | -1.69E-46 |
| MS.gene05847 | MS.gene53579 | 0.826077 | 3.31E-54 | -1.69E-46 |
| MS.gene058548 | MS.gene53579 | 0.85367 | 2.05E-61 | -1.69E-46 |
| MS.gene058549 | MS.gene53579 | 0.844128 | 9.16E-59 | -1.69E-46 |
| MS.gene058595 | MS.gene53579 | 0.817215 | 3.70E-52 | -1.69E-46 |
| MS.gene058625 | MS.gene53579 | 0.807155 | 5.80E-50 | -1.69E-46 |
| MS.gene058783 | MS.gene53579 | 0.821754 | 3.41E-53 | -1.69E-46 |
| MS.gene058979 | MS.gene53579 | 0.848234 | 6.98E-60 | -1.69E-46 |
| MS.gene05897 | MS.gene53579 | 0.814064 | 1.86E-51 | -1.69E-46 |
| MS.gene059006 | MS.gene53579 | 0.808395 | 3.16E-50 | -1.69E-46 |
| MS.gene059151 | MS.gene53579 | 0.825234 | 5.24E-54 | -1.69E-46 |
| MS.gene059161 | MS.gene53579 | 0.812203 | 4.77E-51 | -1.69E-46 |
| MS.gene059283 | MS.gene53579 | 0.811692 | 6.16E-51 | -1.69E-46 |
| MS.gene059317 | MS.gene53579 | 0.823191 | 1.58E-53 | -1.69E-46 |
| MS.gene05954 | MS.gene53579 | 0.830146 | 3.46E-55 | -1.69E-46 |
| MS.gene059569 | MS.gene53579 | 0.826441 | 2.71E-54 | -1.69E-46 |
| MS.gene059625 | MS.gene53579 | 0.81076 | 9.82E-51 | -1.69E-46 |
| MS.gene059725 | MS.gene53579 | 0.802663 | 5.04E-49 | -1.69E-46 |
| MS.gene05978 | MS.gene53579 | 0.820408 | 6.97E-53 | -1.69E-46 |
| MS.gene05979 | MS.gene53579 | 0.86876 | 5.14E-66 | -1.69E-46 |
| MS.gene059830 | MS.gene53579 | 0.8339 | 4.09E-56 | -1.69E-46 |
| MS.gene059842 | MS.gene53579 | 0.859351 | 4.39E-63 | -1.69E-46 |
| MS.gene060011 | MS.gene53579 | 0.804715 | 1.89E-49 | -1.69E-46 |
| MS.gene060310 | MS.gene53579 | 0.83044 | 2.93E-55 | -1.69E-46 |
| MS.gene060322 | MS.gene53579 | 0.845359 | 4.27E-59 | -1.69E-46 |
| MS.gene060365 | MS.gene53579 | 0.818015 | 2.44E-52 | -1.69E-46 |
| MS.gene060429 | MS.gene53579 | 0.868659 | 5.54E-66 | -1.69E-46 |
| MS.gene060521 | MS.gene53579 | 0.811022 | 8.62E-51 | -1.69E-46 |
| MS.gene060572 | MS.gene53579 | 0.845599 | 3.67E-59 | -1.69E-46 |
| MS.gene060586 | MS.gene53579 | 0.809536 | 1.80E-50 | -1.69E-46 |
| MS.gene060802 | MS.gene53579 | 0.839966 | 1.16E-57 | -1.69E-46 |
| MS.gene060803 | MS.gene53579 | 0.868919 | 4.56E-66 | -1.69E-46 |
| MS.gene060907 | MS.gene53579 | 0.811449 | 6.96E-51 | -1.69E-46 |
| MS.gene060917 | MS.gene53579 | 0.81952 | 1.11E-52 | -1.69E-46 |
| MS.gene060935 | MS.gene53579 | 0.816921 | 4.31E-52 | -1.69E-46 |
| MS.gene060998 | MS.gene53579 | 0.831063 | 2.06E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53579.t1 | MTR_1g045890 | 94.286 | 35 | 2 | 0 | 1 | 35 | 20 | 54 | 6.88e-17 | 67.4 |
| MS.gene53579.t1 | MTR_1g045890 | 75.000 | 48 | 11 | 1 | 1 | 47 | 20 | 67 | 1.01e-16 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCGACTTTGCTATCATTGA+CGG | 0.418660 | 1.2:-28802124 | None:intergenic |
| ATGAGAACAATGGATTTGTT+CGG | 0.449405 | 1.2:+28802018 | MS.gene53579:CDS |
| ATCATTGACGGCAATACCTT+CGG | 0.457365 | 1.2:-28802112 | None:intergenic |
| GGATTTGTTCGGAGAGGAGA+TGG | 0.472087 | 1.2:+28802029 | MS.gene53579:CDS |
| CAGCATCATCGAAATCATCT+TGG | 0.487074 | 1.2:-28802170 | None:intergenic |
| TCAGATAACAACCATGGCTA+TGG | 0.492608 | 1.2:+28802056 | MS.gene53579:CDS |
| ATCATCGAAATCATCTTGGA+AGG | 0.593027 | 1.2:-28802166 | None:intergenic |
| AACAATGGATTTGTTCGGAG+AGG | 0.601463 | 1.2:+28802023 | MS.gene53579:CDS |
| AATTTCGATATCTTATCCGA+AGG | 0.608255 | 1.2:+28802096 | MS.gene53579:CDS |
| TTTGTTCGGAGAGGAGATGG+AGG | 0.616272 | 1.2:+28802032 | MS.gene53579:CDS |
| GTCATCAACATCCATAGCCA+TGG | 0.620045 | 1.2:-28802067 | None:intergenic |
| CAGTCGAAGAATGAGAACAA+TGG | 0.621340 | 1.2:+28802008 | None:intergenic |
| GGAGGATCAGATAACAACCA+TGG | 0.690661 | 1.2:+28802050 | MS.gene53579:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATTTCGATATCTTATCCGA+AGG | + | chr1.2:28802096-28802115 | MS.gene53579:CDS | 30.0% | |
| ! | ATGAGAACAATGGATTTGTT+CGG | + | chr1.2:28802018-28802037 | MS.gene53579:CDS | 30.0% |
| ATCATCGAAATCATCTTGGA+AGG | - | chr1.2:28802169-28802188 | None:intergenic | 35.0% | |
| ATCATTGACGGCAATACCTT+CGG | - | chr1.2:28802115-28802134 | None:intergenic | 40.0% | |
| CAGCATCATCGAAATCATCT+TGG | - | chr1.2:28802173-28802192 | None:intergenic | 40.0% | |
| TCAGATAACAACCATGGCTA+TGG | + | chr1.2:28802056-28802075 | MS.gene53579:CDS | 40.0% | |
| ! | AACAATGGATTTGTTCGGAG+AGG | + | chr1.2:28802023-28802042 | MS.gene53579:CDS | 40.0% |
| ! | AGCGACTTTGCTATCATTGA+CGG | - | chr1.2:28802127-28802146 | None:intergenic | 40.0% |
| GGAGGATCAGATAACAACCA+TGG | + | chr1.2:28802050-28802069 | MS.gene53579:CDS | 45.0% | |
| GTCATCAACATCCATAGCCA+TGG | - | chr1.2:28802070-28802089 | None:intergenic | 45.0% | |
| TTTGTTCGGAGAGGAGATGG+AGG | + | chr1.2:28802032-28802051 | MS.gene53579:CDS | 50.0% | |
| ! | GGATTTGTTCGGAGAGGAGA+TGG | + | chr1.2:28802029-28802048 | MS.gene53579:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 28802018 | 28802203 | 28802018 | ID=MS.gene53579 |
| chr1.2 | mRNA | 28802018 | 28802203 | 28802018 | ID=MS.gene53579.t1;Parent=MS.gene53579 |
| chr1.2 | exon | 28802018 | 28802203 | 28802018 | ID=MS.gene53579.t1.exon1;Parent=MS.gene53579.t1 |
| chr1.2 | CDS | 28802018 | 28802203 | 28802018 | ID=cds.MS.gene53579.t1;Parent=MS.gene53579.t1 |
| Gene Sequence |
| Protein sequence |
