AlfalfaGEDB | MS.gene58252

M. sativa cultivar XinJiangDaYe / MS.gene58252

KEGG: K00345
GO: GO:0006091 GO:0008150 GO:0008152 GO:0009767 GO:0009987 GO:0022900 GO:0044237 GO:0055114
PFAM: PF11623.11

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58252.t1	XP_003627623.1	93.8	240	15	0	1	240	1	240	3.90E-124	454.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58252.t1	Q9T0A4	54.3	247	98	6	2	241	3	241	3.7e-62	239.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58252.t1	G7LCN2	93.8	240	15	0	1	240	1	240	2.8e-124	454.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049134	MS.gene58252	-0.802204	6.27E-49	-1.69E-46
MS.gene049137	MS.gene58252	0.815969	7.04E-52	-1.69E-46
MS.gene049346	MS.gene58252	0.816275	6.01E-52	-1.69E-46
MS.gene049351	MS.gene58252	0.804883	1.74E-49	-1.69E-46
MS.gene049356	MS.gene58252	0.854224	1.42E-61	-1.69E-46
MS.gene049369	MS.gene58252	0.815352	9.66E-52	-1.69E-46
MS.gene049427	MS.gene58252	0.824146	9.45E-54	-1.69E-46
MS.gene049488	MS.gene58252	0.824892	6.31E-54	-1.69E-46
MS.gene049491	MS.gene58252	0.808312	3.30E-50	-1.69E-46
MS.gene049609	MS.gene58252	0.827352	1.64E-54	-1.69E-46
MS.gene049661	MS.gene58252	0.844097	9.33E-59	-1.69E-46
MS.gene049662	MS.gene58252	0.844124	9.18E-59	-1.69E-46
MS.gene049758	MS.gene58252	0.811253	7.68E-51	-1.69E-46
MS.gene049805	MS.gene58252	0.815251	1.02E-51	-1.69E-46
MS.gene049910	MS.gene58252	0.811531	6.68E-51	-1.69E-46
MS.gene050122	MS.gene58252	0.805591	1.24E-49	-1.69E-46
MS.gene050301	MS.gene58252	0.849998	2.26E-60	-1.69E-46
MS.gene050397	MS.gene58252	0.849981	2.28E-60	-1.69E-46
MS.gene050400	MS.gene58252	0.805742	1.15E-49	-1.69E-46
MS.gene050406	MS.gene58252	0.826391	2.78E-54	-1.69E-46
MS.gene050426	MS.gene58252	-0.832307	1.02E-55	-1.69E-46
MS.gene050432	MS.gene58252	0.846093	2.70E-59	-1.69E-46
MS.gene050464	MS.gene58252	0.852957	3.28E-61	-1.69E-46
MS.gene050529	MS.gene58252	0.847568	1.06E-59	-1.69E-46
MS.gene050593	MS.gene58252	0.861397	1.06E-63	-1.69E-46
MS.gene050625	MS.gene58252	0.808043	3.76E-50	-1.69E-46
MS.gene050670	MS.gene58252	0.930303	1.99E-93	-1.69E-46
MS.gene050772	MS.gene58252	0.80132	9.52E-49	-1.69E-46
MS.gene050971	MS.gene58252	0.804363	2.24E-49	-1.69E-46
MS.gene05101	MS.gene58252	0.836674	8.15E-57	-1.69E-46
MS.gene05105	MS.gene58252	0.90145	2.65E-78	-1.69E-46
MS.gene051360	MS.gene58252	0.802832	4.65E-49	-1.69E-46
MS.gene051387	MS.gene58252	0.852845	3.53E-61	-1.69E-46
MS.gene051525	MS.gene58252	0.865196	7.04E-65	-1.69E-46
MS.gene051582	MS.gene58252	0.874265	7.73E-68	-1.69E-46
MS.gene051605	MS.gene58252	0.818575	1.82E-52	-1.69E-46
MS.gene051666	MS.gene58252	0.83875	2.39E-57	-1.69E-46
MS.gene051692	MS.gene58252	0.828495	8.71E-55	-1.69E-46
MS.gene051860	MS.gene58252	0.81247	4.17E-51	-1.69E-46
MS.gene052011	MS.gene58252	-0.846036	2.79E-59	-1.69E-46
MS.gene052077	MS.gene58252	0.831896	1.29E-55	-1.69E-46
MS.gene052078	MS.gene58252	0.80799	3.86E-50	-1.69E-46
MS.gene052134	MS.gene58252	0.806487	8.03E-50	-1.69E-46
MS.gene052135	MS.gene58252	0.823673	1.22E-53	-1.69E-46
MS.gene052136	MS.gene58252	0.812825	3.49E-51	-1.69E-46
MS.gene052188	MS.gene58252	0.882142	1.33E-70	-1.69E-46
MS.gene052253	MS.gene58252	0.885461	7.97E-72	-1.69E-46
MS.gene052310	MS.gene58252	0.876428	1.41E-68	-1.69E-46
MS.gene052322	MS.gene58252	0.835413	1.70E-56	-1.69E-46
MS.gene052340	MS.gene58252	0.87835	3.01E-69	-1.69E-46
MS.gene052346	MS.gene58252	0.812339	4.45E-51	-1.69E-46
MS.gene052414	MS.gene58252	0.860221	2.40E-63	-1.69E-46
MS.gene052421	MS.gene58252	0.849087	4.05E-60	-1.69E-46
MS.gene052481	MS.gene58252	0.864615	1.07E-64	-1.69E-46
MS.gene052484	MS.gene58252	0.818207	2.21E-52	-1.69E-46
MS.gene052485	MS.gene58252	0.867183	1.65E-65	-1.69E-46
MS.gene052501	MS.gene58252	0.824101	9.69E-54	-1.69E-46
MS.gene052505	MS.gene58252	0.835931	1.26E-56	-1.69E-46
MS.gene052982	MS.gene58252	0.841742	3.95E-58	-1.69E-46
MS.gene053103	MS.gene58252	0.827363	1.63E-54	-1.69E-46
MS.gene053104	MS.gene58252	0.810984	8.78E-51	-1.69E-46
MS.gene053169	MS.gene58252	0.823603	1.27E-53	-1.69E-46
MS.gene053207	MS.gene58252	0.805428	1.34E-49	-1.69E-46
MS.gene053209	MS.gene58252	0.854519	1.17E-61	-1.69E-46
MS.gene053449	MS.gene58252	0.847715	9.70E-60	-1.69E-46
MS.gene053557	MS.gene58252	0.814677	1.36E-51	-1.69E-46
MS.gene053574	MS.gene58252	0.808906	2.46E-50	-1.69E-46
MS.gene053758	MS.gene58252	0.82818	1.04E-54	-1.69E-46
MS.gene053813	MS.gene58252	0.820718	5.92E-53	-1.69E-46
MS.gene053836	MS.gene58252	0.84431	8.18E-59	-1.69E-46
MS.gene053873	MS.gene58252	0.805614	1.23E-49	-1.69E-46
MS.gene053912	MS.gene58252	0.831309	1.80E-55	-1.69E-46
MS.gene053928	MS.gene58252	0.813944	1.98E-51	-1.69E-46
MS.gene054010	MS.gene58252	0.800882	1.17E-48	-1.69E-46
MS.gene054011	MS.gene58252	0.801591	8.38E-49	-1.69E-46
MS.gene054123	MS.gene58252	0.822838	1.91E-53	-1.69E-46
MS.gene054183	MS.gene58252	0.839778	1.29E-57	-1.69E-46
MS.gene054349	MS.gene58252	0.830442	2.93E-55	-1.69E-46
MS.gene054556	MS.gene58252	0.83783	4.12E-57	-1.69E-46
MS.gene054719	MS.gene58252	0.800321	1.52E-48	-1.69E-46
MS.gene054917	MS.gene58252	0.803998	2.67E-49	-1.69E-46
MS.gene054918	MS.gene58252	0.841251	5.32E-58	-1.69E-46
MS.gene054927	MS.gene58252	0.803705	3.07E-49	-1.69E-46
MS.gene054955	MS.gene58252	0.824523	7.71E-54	-1.69E-46
MS.gene05505	MS.gene58252	0.826839	2.18E-54	-1.69E-46
MS.gene055116	MS.gene58252	0.941806	2.19E-101	-1.69E-46
MS.gene055277	MS.gene58252	0.833767	4.41E-56	-1.69E-46
MS.gene055369	MS.gene58252	0.85621	3.75E-62	-1.69E-46
MS.gene055422	MS.gene58252	0.817435	3.30E-52	-1.69E-46
MS.gene055428	MS.gene58252	0.822757	2.00E-53	-1.69E-46
MS.gene055539	MS.gene58252	0.814337	1.62E-51	-1.69E-46
MS.gene05553	MS.gene58252	0.851479	8.64E-61	-1.69E-46
MS.gene055564	MS.gene58252	0.828067	1.10E-54	-1.69E-46
MS.gene055604	MS.gene58252	0.810234	1.28E-50	-1.69E-46
MS.gene055722	MS.gene58252	0.802556	5.30E-49	-1.69E-46
MS.gene055741	MS.gene58252	0.813027	3.15E-51	-1.69E-46
MS.gene055744	MS.gene58252	0.823272	1.51E-53	-1.69E-46
MS.gene055859	MS.gene58252	0.816681	4.88E-52	-1.69E-46
MS.gene055909	MS.gene58252	0.832636	8.44E-56	-1.69E-46
MS.gene055911	MS.gene58252	0.839644	1.40E-57	-1.69E-46
MS.gene05592	MS.gene58252	0.817413	3.34E-52	-1.69E-46
MS.gene05594	MS.gene58252	0.827936	1.19E-54	-1.69E-46
MS.gene05602	MS.gene58252	0.800582	1.35E-48	-1.69E-46
MS.gene056191	MS.gene58252	0.861869	7.58E-64	-1.69E-46
MS.gene056337	MS.gene58252	0.829429	5.17E-55	-1.69E-46
MS.gene056407	MS.gene58252	0.89981	1.37E-77	-1.69E-46
MS.gene056410	MS.gene58252	0.817312	3.52E-52	-1.69E-46
MS.gene056422	MS.gene58252	0.819745	9.88E-53	-1.69E-46
MS.gene056511	MS.gene58252	0.892782	1.16E-74	-1.69E-46
MS.gene056514	MS.gene58252	0.843585	1.28E-58	-1.69E-46
MS.gene056609	MS.gene58252	0.801579	8.42E-49	-1.69E-46
MS.gene056612	MS.gene58252	0.820105	8.18E-53	-1.69E-46
MS.gene05664	MS.gene58252	0.823329	1.47E-53	-1.69E-46
MS.gene056659	MS.gene58252	0.836083	1.15E-56	-1.69E-46
MS.gene05666	MS.gene58252	0.818636	1.77E-52	-1.69E-46
MS.gene056723	MS.gene58252	0.849469	3.17E-60	-1.69E-46
MS.gene056877	MS.gene58252	0.803467	3.44E-49	-1.69E-46
MS.gene056899	MS.gene58252	-0.81986	9.30E-53	-1.69E-46
MS.gene05702	MS.gene58252	0.872959	2.13E-67	-1.69E-46
MS.gene057076	MS.gene58252	0.80629	8.84E-50	-1.69E-46
MS.gene057123	MS.gene58252	0.812502	4.10E-51	-1.69E-46
MS.gene057124	MS.gene58252	0.869604	2.73E-66	-1.69E-46
MS.gene057146	MS.gene58252	0.827767	1.30E-54	-1.69E-46
MS.gene057330	MS.gene58252	0.810258	1.26E-50	-1.69E-46
MS.gene057331	MS.gene58252	0.82044	6.85E-53	-1.69E-46
MS.gene05735	MS.gene58252	0.802281	6.04E-49	-1.69E-46
MS.gene057396	MS.gene58252	0.838527	2.73E-57	-1.69E-46
MS.gene057416	MS.gene58252	0.819163	1.34E-52	-1.69E-46
MS.gene057448	MS.gene58252	0.846604	1.96E-59	-1.69E-46
MS.gene057449	MS.gene58252	0.847416	1.17E-59	-1.69E-46
MS.gene057450	MS.gene58252	0.841724	4.00E-58	-1.69E-46
MS.gene057476	MS.gene58252	0.845727	3.39E-59	-1.69E-46
MS.gene057734	MS.gene58252	0.842684	2.22E-58	-1.69E-46
MS.gene057749	MS.gene58252	0.886185	4.26E-72	-1.69E-46
MS.gene057823	MS.gene58252	0.877155	7.88E-69	-1.69E-46
MS.gene057825	MS.gene58252	0.828061	1.11E-54	-1.69E-46
MS.gene057888	MS.gene58252	0.807841	4.15E-50	-1.69E-46
MS.gene058002	MS.gene58252	0.86077	1.64E-63	-1.69E-46
MS.gene058006	MS.gene58252	0.802612	5.16E-49	-1.69E-46
MS.gene058007	MS.gene58252	0.850549	1.58E-60	-1.69E-46
MS.gene058019	MS.gene58252	0.85793	1.17E-62	-1.69E-46
MS.gene058082	MS.gene58252	0.809694	1.67E-50	-1.69E-46
MS.gene05811	MS.gene58252	0.819653	1.04E-52	-1.69E-46
MS.gene058142	MS.gene58252	0.803827	2.90E-49	-1.69E-46
MS.gene058233	MS.gene58252	0.836261	1.04E-56	-1.69E-46
MS.gene058293	MS.gene58252	0.843043	1.79E-58	-1.69E-46
MS.gene058365	MS.gene58252	0.825653	4.17E-54	-1.69E-46
MS.gene058376	MS.gene58252	0.8284	9.18E-55	-1.69E-46
MS.gene058444	MS.gene58252	0.822059	2.90E-53	-1.69E-46
MS.gene058660	MS.gene58252	0.864784	9.48E-65	-1.69E-46
MS.gene058700	MS.gene58252	0.852298	5.06E-61	-1.69E-46
MS.gene058710	MS.gene58252	0.829593	4.72E-55	-1.69E-46
MS.gene058787	MS.gene58252	0.807347	5.28E-50	-1.69E-46
MS.gene05886	MS.gene58252	0.846606	1.95E-59	-1.69E-46
MS.gene058928	MS.gene58252	0.857274	1.82E-62	-1.69E-46
MS.gene058948	MS.gene58252	0.818241	2.17E-52	-1.69E-46
MS.gene058949	MS.gene58252	0.837433	5.21E-57	-1.69E-46
MS.gene058995	MS.gene58252	0.843967	1.01E-58	-1.69E-46
MS.gene059011	MS.gene58252	0.855946	4.48E-62	-1.69E-46
MS.gene05907	MS.gene58252	0.877399	6.48E-69	-1.69E-46
MS.gene059082	MS.gene58252	0.839139	1.89E-57	-1.69E-46
MS.gene059141	MS.gene58252	0.837982	3.77E-57	-1.69E-46
MS.gene059285	MS.gene58252	0.835941	1.25E-56	-1.69E-46
MS.gene059294	MS.gene58252	0.828941	6.79E-55	-1.69E-46
MS.gene059366	MS.gene58252	0.837282	5.70E-57	-1.69E-46
MS.gene059420	MS.gene58252	0.886807	2.48E-72	-1.69E-46
MS.gene059455	MS.gene58252	0.81702	4.09E-52	-1.69E-46
MS.gene059462	MS.gene58252	0.851398	9.11E-61	-1.69E-46
MS.gene059536	MS.gene58252	0.831901	1.28E-55	-1.69E-46
MS.gene059597	MS.gene58252	0.839323	1.70E-57	-1.69E-46
MS.gene059674	MS.gene58252	0.801691	7.99E-49	-1.69E-46
MS.gene059676	MS.gene58252	0.848647	5.36E-60	-1.69E-46
MS.gene059688	MS.gene58252	0.914596	1.59E-84	-1.69E-46
MS.gene059692	MS.gene58252	0.898144	7.07E-77	-1.69E-46
MS.gene059695	MS.gene58252	0.901865	1.74E-78	-1.69E-46
MS.gene059711	MS.gene58252	0.819969	8.79E-53	-1.69E-46
MS.gene059807	MS.gene58252	0.853842	1.83E-61	-1.69E-46
MS.gene05984	MS.gene58252	0.83495	2.23E-56	-1.69E-46
MS.gene059876	MS.gene58252	0.855887	4.66E-62	-1.69E-46
MS.gene059894	MS.gene58252	0.802671	5.02E-49	-1.69E-46
MS.gene059933	MS.gene58252	0.81347	2.52E-51	-1.69E-46
MS.gene060247	MS.gene58252	0.800295	1.54E-48	-1.69E-46
MS.gene060372	MS.gene58252	0.800091	1.70E-48	-1.69E-46
MS.gene06037	MS.gene58252	0.817834	2.69E-52	-1.69E-46
MS.gene060403	MS.gene58252	0.903673	2.72E-79	-1.69E-46
MS.gene060804	MS.gene58252	0.860785	1.62E-63	-1.69E-46
MS.gene060835	MS.gene58252	0.836696	8.04E-57	-1.69E-46
MS.gene060837	MS.gene58252	0.852946	3.31E-61	-1.69E-46
MS.gene061041	MS.gene58252	0.818574	1.83E-52	-1.69E-46
MS.gene061049	MS.gene58252	0.870583	1.31E-66	-1.69E-46
MS.gene061051	MS.gene58252	-0.854207	1.44E-61	-1.69E-46
MS.gene061154	MS.gene58252	0.864306	1.34E-64	-1.69E-46
MS.gene061159	MS.gene58252	0.839502	1.52E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58252.t1	MTR_8g032260	93.750	240	15	0	1	240	1	240	1.34e-167	462
MS.gene58252.t1	MTR_7g076620	47.183	142	68	3	1	142	1	135	9.51e-37	127

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58252.t1	AT4G23890	55.466	247	95	6	2	241	3	241	9.17e-85	253

Find 59 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTCCGACGAAGAAAGTTTA+AGG	0.169983	8.4:-64408593	None:intergenic
GTTGCCATTCATAGCCTTCA+TGG	0.252632	8.4:+64408150	MS.gene58252:CDS
GGGTTTCCTGGTGGTGAAAA+AGG	0.277133	8.4:+64408513	MS.gene58252:CDS
ATACCACAGTACATGTAAAA+TGG	0.281303	8.4:-64408684	None:intergenic
TTGTCACCTCTACCAGGTTT+TGG	0.284174	8.4:-64408567	None:intergenic
GATCTGCAGCAAGTCGAATT+TGG	0.288631	8.4:-64408289	None:intergenic
GAACTTGAGAGAAGAGAAAA+AGG	0.294463	8.4:+64408789	MS.gene58252:CDS
GGGTTAACTGGAGGGTTTCC+TGG	0.308952	8.4:+64408501	MS.gene58252:CDS
TATGATTTCTTTACAAGAAA+TGG	0.313320	8.4:-64408849	None:intergenic
AAAGAGTTGATGGGGTTAAC+TGG	0.314813	8.4:+64408489	MS.gene58252:CDS
TACTGTTTGAAGGAGGGAAT+TGG	0.328753	8.4:+64408739	MS.gene58252:CDS
ACTGTTTGAAGGAGGGAATT+GGG	0.336476	8.4:+64408740	MS.gene58252:CDS
GGGATAAGTTGATCACCTTT+AGG	0.350751	8.4:+64408760	MS.gene58252:CDS
AGTCGAATTTGGCACATGTT+TGG	0.374390	8.4:-64408278	None:intergenic
TTCACAATAGCAATCATTCC+TGG	0.392991	8.4:-64408648	None:intergenic
TTCGAAAGTGTTACAGAAGA+AGG	0.394068	8.4:+64408459	MS.gene58252:CDS
AGGGTTTGATAAAGAGTTGA+TGG	0.404058	8.4:+64408479	MS.gene58252:CDS
GAACTTCCTATGTTACTTCC+AGG	0.411204	8.4:+64408630	MS.gene58252:CDS
ATTCGACTTGCTGCAGATCA+TGG	0.411694	8.4:+64408293	MS.gene58252:CDS
CAAGACTTAGGATTCTTCAT+AGG	0.437208	8.4:-64408816	None:intergenic
ATTCTCTTGGTCTAAGAACT+TGG	0.444203	8.4:-64408187	None:intergenic
GGAAGTAACATAGGAAGTTC+CGG	0.448635	8.4:-64408627	None:intergenic
AAAGAGCTTAAAAGAGAAGT+TGG	0.459053	8.4:+64408354	MS.gene58252:CDS
GGGTTTGATAAAGAGTTGAT+GGG	0.470608	8.4:+64408480	MS.gene58252:CDS
GGTTCAAGAACACAAGACTT+AGG	0.485751	8.4:-64408828	None:intergenic
TTGCTTGCTTCTAATGATGT+TGG	0.487661	8.4:-64408396	None:intergenic
GGACTCTGCAAAGGAGAAGA+AGG	0.493957	8.4:+64408324	MS.gene58252:CDS
TAAACTTTCTTCGTCGGAGA+AGG	0.497501	8.4:+64408596	MS.gene58252:CDS
CTGCAGATCATGGGAGGCAG+AGG	0.507026	8.4:+64408303	MS.gene58252:CDS
AGAATCACTGATGGTAAAGC+AGG	0.515714	8.4:+64408714	MS.gene58252:CDS
CTCAAGTTCCTCGAGCCTAA+AGG	0.519479	8.4:-64408775	None:intergenic
AGTTGATGGGGTTAACTGGA+GGG	0.519714	8.4:+64408493	MS.gene58252:CDS
TTCGACTTGCTGCAGATCAT+GGG	0.525619	8.4:+64408294	MS.gene58252:CDS
TCGAAAGTGTTACAGAAGAA+GGG	0.527595	8.4:+64408460	MS.gene58252:CDS
GACTTAGGATTCTTCATAGG+AGG	0.531635	8.4:-64408813	None:intergenic
GAAAACCCTGTTCCAAAACC+TGG	0.534181	8.4:+64408555	MS.gene58252:CDS
AAAGATGAGTGAGATTCTCT+TGG	0.534612	8.4:-64408200	None:intergenic
AAGCCTTAAACTTTCTTCGT+CGG	0.537850	8.4:+64408590	MS.gene58252:CDS
GGTCGGTTTGCTGTGAAAGA+TGG	0.548431	8.4:-64408244	None:intergenic
AAAGCAGGAGTACTGTTTGA+AGG	0.555664	8.4:+64408729	MS.gene58252:CDS
ATCATTCCTGGAAGTAACAT+AGG	0.560101	8.4:-64408636	None:intergenic
TTCTTCTGTAACACTTTCGA+AGG	0.563507	8.4:-64408457	None:intergenic
TTCTAAGAAGAGAGCCATGA+AGG	0.565277	8.4:-64408164	None:intergenic
GAGTTGATGGGGTTAACTGG+AGG	0.584401	8.4:+64408492	MS.gene58252:CDS
GGTTTGATAAAGAGTTGATG+GGG	0.584525	8.4:+64408481	MS.gene58252:CDS
AGAGCCATGAAGGCTATGAA+TGG	0.586096	8.4:-64408154	None:intergenic
AAGGCTATGAATGGCAACAA+AGG	0.593054	8.4:-64408145	None:intergenic
GAATTTGGCACATGTTTGGA+CGG	0.594746	8.4:-64408274	None:intergenic
GCAGGAGTACTGTTTGAAGG+AGG	0.603818	8.4:+64408732	MS.gene58252:CDS
TTAACTGGAGGGTTTCCTGG+TGG	0.603897	8.4:+64408504	MS.gene58252:CDS
CCTGTTCCAAAACCTGGTAG+AGG	0.604649	8.4:+64408561	MS.gene58252:CDS
GTTGATCACCTTTAGGCTCG+AGG	0.610953	8.4:+64408767	MS.gene58252:CDS
GTCGGAGAAGGCAAAACCAC+CGG	0.613095	8.4:+64408608	MS.gene58252:CDS
CAGGAGTACTGTTTGAAGGA+GGG	0.618790	8.4:+64408733	MS.gene58252:CDS
ATTGTTCAAAGAATCACTGA+TGG	0.621968	8.4:+64408705	MS.gene58252:CDS
GGAGGCAGAGGACTCTGCAA+AGG	0.631448	8.4:+64408315	MS.gene58252:CDS
GACTTGCTGCAGATCATGGG+AGG	0.640143	8.4:+64408297	MS.gene58252:CDS
ATGGAAGCTTTATTGCTGTG+AGG	0.676809	8.4:-64408225	None:intergenic
AGTAACATAGGAAGTTCCGG+TGG	0.715020	8.4:-64408624	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATGATTTCTTTACAAGAAA+TGG	-	chr8.4:64408852-64408871	None:intergenic	20.0%
	AAAGAGCTTAAAAGAGAAGT+TGG	+	chr8.4:64408354-64408373	MS.gene58252:CDS	30.0%
	ATACCACAGTACATGTAAAA+TGG	-	chr8.4:64408687-64408706	None:intergenic	30.0%
	ATTGTTCAAAGAATCACTGA+TGG	+	chr8.4:64408705-64408724	MS.gene58252:CDS	30.0%
!	AATCCATTTTACATGTACTG+TGG	+	chr8.4:64408681-64408700	MS.gene58252:CDS	30.0%
	AAAGATGAGTGAGATTCTCT+TGG	-	chr8.4:64408203-64408222	None:intergenic	35.0%
	AAGCCTTAAACTTTCTTCGT+CGG	+	chr8.4:64408590-64408609	MS.gene58252:CDS	35.0%
	AGGGTTTGATAAAGAGTTGA+TGG	+	chr8.4:64408479-64408498	MS.gene58252:CDS	35.0%
	ATCATTCCTGGAAGTAACAT+AGG	-	chr8.4:64408639-64408658	None:intergenic	35.0%
	ATTCTCTTGGTCTAAGAACT+TGG	-	chr8.4:64408190-64408209	None:intergenic	35.0%
	CAAGACTTAGGATTCTTCAT+AGG	-	chr8.4:64408819-64408838	None:intergenic	35.0%
	GAACTTGAGAGAAGAGAAAA+AGG	+	chr8.4:64408789-64408808	MS.gene58252:CDS	35.0%
	TTCACAATAGCAATCATTCC+TGG	-	chr8.4:64408651-64408670	None:intergenic	35.0%
	TTCTTCTGTAACACTTTCGA+AGG	-	chr8.4:64408460-64408479	None:intergenic	35.0%
	TTGCTTGCTTCTAATGATGT+TGG	-	chr8.4:64408399-64408418	None:intergenic	35.0%
!	GGGTTTGATAAAGAGTTGAT+GGG	+	chr8.4:64408480-64408499	MS.gene58252:CDS	35.0%
!!	GGTTTGATAAAGAGTTGATG+GGG	+	chr8.4:64408481-64408500	MS.gene58252:CDS	35.0%
!!	TCGAAAGTGTTACAGAAGAA+GGG	+	chr8.4:64408460-64408479	MS.gene58252:CDS	35.0%
!!	TTCGAAAGTGTTACAGAAGA+AGG	+	chr8.4:64408459-64408478	MS.gene58252:CDS	35.0%
	AAGGCTATGAATGGCAACAA+AGG	-	chr8.4:64408148-64408167	None:intergenic	40.0%
	ACTGTTTGAAGGAGGGAATT+GGG	+	chr8.4:64408740-64408759	MS.gene58252:CDS	40.0%
	AGAATCACTGATGGTAAAGC+AGG	+	chr8.4:64408714-64408733	MS.gene58252:CDS	40.0%
	AGTCGAATTTGGCACATGTT+TGG	-	chr8.4:64408281-64408300	None:intergenic	40.0%
	GAATTTGGCACATGTTTGGA+CGG	-	chr8.4:64408277-64408296	None:intergenic	40.0%
	GACTTAGGATTCTTCATAGG+AGG	-	chr8.4:64408816-64408835	None:intergenic	40.0%
	GGAAGTAACATAGGAAGTTC+CGG	-	chr8.4:64408630-64408649	None:intergenic	40.0%
	GGTTCAAGAACACAAGACTT+AGG	-	chr8.4:64408831-64408850	None:intergenic	40.0%
	TAAACTTTCTTCGTCGGAGA+AGG	+	chr8.4:64408596-64408615	MS.gene58252:CDS	40.0%
	TACTGTTTGAAGGAGGGAAT+TGG	+	chr8.4:64408739-64408758	MS.gene58252:CDS	40.0%
	TCTCCGACGAAGAAAGTTTA+AGG	-	chr8.4:64408596-64408615	None:intergenic	40.0%
!	GAACTTCCTATGTTACTTCC+AGG	+	chr8.4:64408630-64408649	MS.gene58252:CDS	40.0%
!	TTCTAAGAAGAGAGCCATGA+AGG	-	chr8.4:64408167-64408186	None:intergenic	40.0%
!!	AAAGAGTTGATGGGGTTAAC+TGG	+	chr8.4:64408489-64408508	MS.gene58252:CDS	40.0%
!!	AAAGCAGGAGTACTGTTTGA+AGG	+	chr8.4:64408729-64408748	MS.gene58252:CDS	40.0%
!!	ACATGTTTGGACGGTTTTGT+AGG	-	chr8.4:64408268-64408287	None:intergenic	40.0%
!!	ATGGAAGCTTTATTGCTGTG+AGG	-	chr8.4:64408228-64408247	None:intergenic	40.0%
!!	GGGATAAGTTGATCACCTTT+AGG	+	chr8.4:64408760-64408779	MS.gene58252:CDS	40.0%
!!	TTCAAACCTTTTTCACCACC+AGG	-	chr8.4:64408522-64408541	None:intergenic	40.0%
	AGAGCCATGAAGGCTATGAA+TGG	-	chr8.4:64408157-64408176	None:intergenic	45.0%
	AGTAACATAGGAAGTTCCGG+TGG	-	chr8.4:64408627-64408646	None:intergenic	45.0%
	GAAAACCCTGTTCCAAAACC+TGG	+	chr8.4:64408555-64408574	MS.gene58252:CDS	45.0%
	GATCTGCAGCAAGTCGAATT+TGG	-	chr8.4:64408292-64408311	None:intergenic	45.0%
	GTTGCCATTCATAGCCTTCA+TGG	+	chr8.4:64408150-64408169	MS.gene58252:CDS	45.0%
!	TTGTCACCTCTACCAGGTTT+TGG	-	chr8.4:64408570-64408589	None:intergenic	45.0%
!!	AGTTGATGGGGTTAACTGGA+GGG	+	chr8.4:64408493-64408512	MS.gene58252:CDS	45.0%
!!	ATTCGACTTGCTGCAGATCA+TGG	+	chr8.4:64408293-64408312	MS.gene58252:CDS	45.0%
!!	CAGGAGTACTGTTTGAAGGA+GGG	+	chr8.4:64408733-64408752	MS.gene58252:CDS	45.0%
!!	GTTTGGACGGTTTTGTAGGT+CGG	-	chr8.4:64408264-64408283	None:intergenic	45.0%
!!	TTCGACTTGCTGCAGATCAT+GGG	+	chr8.4:64408294-64408313	MS.gene58252:CDS	45.0%
!!!	CTCTACCAGGTTTTGGAACA+GGG	-	chr8.4:64408563-64408582	None:intergenic	45.0%
	CCTGTTCCAAAACCTGGTAG+AGG	+	chr8.4:64408561-64408580	MS.gene58252:CDS	50.0%
	CTCAAGTTCCTCGAGCCTAA+AGG	-	chr8.4:64408778-64408797	None:intergenic	50.0%
	GGACTCTGCAAAGGAGAAGA+AGG	+	chr8.4:64408324-64408343	MS.gene58252:CDS	50.0%
	GTTGATCACCTTTAGGCTCG+AGG	+	chr8.4:64408767-64408786	MS.gene58252:CDS	50.0%
	TTAACTGGAGGGTTTCCTGG+TGG	+	chr8.4:64408504-64408523	MS.gene58252:CDS	50.0%
!	AGGCTTTTGTCACCTCTACC+AGG	-	chr8.4:64408576-64408595	None:intergenic	50.0%
!	GGGTTTCCTGGTGGTGAAAA+AGG	+	chr8.4:64408513-64408532	MS.gene58252:CDS	50.0%
!!	GAGTTGATGGGGTTAACTGG+AGG	+	chr8.4:64408492-64408511	MS.gene58252:CDS	50.0%
!!	GCAGGAGTACTGTTTGAAGG+AGG	+	chr8.4:64408732-64408751	MS.gene58252:CDS	50.0%
!!	GGTCGGTTTGCTGTGAAAGA+TGG	-	chr8.4:64408247-64408266	None:intergenic	50.0%
!!!	CCTCTACCAGGTTTTGGAAC+AGG	-	chr8.4:64408564-64408583	None:intergenic	50.0%
	GGGTTAACTGGAGGGTTTCC+TGG	+	chr8.4:64408501-64408520	MS.gene58252:CDS	55.0%
	GTCGGAGAAGGCAAAACCAC+CGG	+	chr8.4:64408608-64408627	MS.gene58252:CDS	55.0%
!!	GACTTGCTGCAGATCATGGG+AGG	+	chr8.4:64408297-64408316	MS.gene58252:CDS	55.0%
	CTGCAGATCATGGGAGGCAG+AGG	+	chr8.4:64408303-64408322	MS.gene58252:CDS	60.0%
	GGAGGCAGAGGACTCTGCAA+AGG	+	chr8.4:64408315-64408334	MS.gene58252:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	64408138	64408997	64408138	ID=MS.gene58252
chr8.4	mRNA	64408138	64408997	64408138	ID=MS.gene58252.t1;Parent=MS.gene58252
chr8.4	exon	64408138	64408857	64408138	ID=MS.gene58252.t1.exon1;Parent=MS.gene58252.t1
chr8.4	CDS	64408138	64408857	64408138	ID=cds.MS.gene58252.t1;Parent=MS.gene58252.t1
chr8.4	exon	64408992	64408997	64408992	ID=MS.gene58252.t1.exon2;Parent=MS.gene58252.t1
chr8.4	CDS	64408992	64408997	64408992	ID=cds.MS.gene58252.t1;Parent=MS.gene58252.t1

Gene Sequence

>MS.gene58252

ATGTCTTCCTTTGTTGCCATTCATAGCCTTCATGGCTCTCTTCTTAGAACCAAGTTCTTAGACCAAGAGAATCTCACTCATCTTTATCCTCACAGCAATAAAGCTTCCATCTTTCACAGCAAACCGACCTACAAAACCGTCCAAACATGTGCCAAATTCGACTTGCTGCAGATCATGGGAGGCAGAGGACTCTGCAAAGGAGAAGAAGGTCTTCAAAAAGAGCTTAAAAGAGAAGTTGGTCTTGATGATCAGAATCAACCAACATCATTAGAAGCAAGCAACGAAGAACAAGAAGACTCGACAAGTAACTCAACAAAATCCTTCGAAAGTGTTACAGAAGAAGGGTTTGATAAAGAGTTGATGGGGTTAACTGGAGGGTTTCCTGGTGGTGAAAAAGGTTTGAAAAAATTCATTGAAGAAAACCCTGTTCCAAAACCTGGTAGAGGTGACAAAAGCCTTAAACTTTCTTCGTCGGAGAAGGCAAAACCACCGGAACTTCCTATGTTACTTCCAGGAATGATTGCTATTGTGAAGAATCAAAATAATCCATTTTACATGTACTGTGGTATTGTTCAAAGAATCACTGATGGTAAAGCAGGAGTACTGTTTGAAGGAGGGAATTGGGATAAGTTGATCACCTTTAGGCTCGAGGAACTTGAGAGAAGAGAAAAAGGTCCTCCTATGAAGAATCCTAAGTCTTGTGTTCTTGAACCATTTCTTGTAAAGAAATCATAGTTTTCAGCTCTTTGTTGTTATTTTGTAACAAAACATGTTCACTTTTGTATCATATTACATGTTTCTGACAATTGAATCTTAGTTGTGTATTTCATTTGCTGCTTTGATCTTTTATTTAGAAGTAA

Protein sequence

>MS.gene58252.t1

MSSFVAIHSLHGSLLRTKFLDQENLTHLYPHSNKASIFHSKPTYKTVQTCAKFDLLQIMGGRGLCKGEEGLQKELKREVGLDDQNQPTSLEASNEEQEDSTSNSTKSFESVTEEGFDKELMGLTGGFPGGEKGLKKFIEENPVPKPGRGDKSLKLSSSEKAKPPELPMLLPGMIAIVKNQNNPFYMYCGIVQRITDGKAGVLFEGGNWDKLITFRLEELERREKGPPMKNPKSCVLEPFLK