AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0113340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0113340 MtrunA17_Chr1g0171171 96.800 375 12 0 1 375 1 375 0.0 764
Msa0113340 MtrunA17_Chr1g0210771 74.792 361 86 3 19 375 23 382 0.0 577
Msa0113340 MtrunA17_Chr5g0430371 39.316 351 190 5 45 375 52 399 3.37e-96 292
Msa0113340 MtrunA17_Chr2g0283581 41.979 374 189 9 9 375 6 358 6.06e-95 287
Msa0113340 MtrunA17_Chr7g0258851 41.449 345 185 6 48 375 243 587 7.93e-94 292
Msa0113340 MtrunA17_Chr2g0325481 42.560 336 173 7 47 375 44 366 5.44e-93 283
Msa0113340 MtrunA17_Chr1g0175671 40.870 345 187 6 48 375 165 509 8.91e-92 285
Msa0113340 MtrunA17_Chr2g0314801 41.791 335 177 5 49 375 29 353 1.28e-91 279
Msa0113340 MtrunA17_Chr3g0097891 43.202 331 169 8 53 375 31 350 3.80e-91 277
Msa0113340 MtrunA17_Chr2g0314791 43.114 334 162 8 53 375 29 345 3.83e-90 275
Msa0113340 MtrunA17_Chr3g0105301 38.988 336 190 5 53 375 43 376 6.77e-88 270
Msa0113340 MtrunA17_Chr5g0413851 38.667 375 204 10 11 375 1 359 2.02e-87 268
Msa0113340 MtrunA17_Chr4g0045941 39.521 334 185 5 48 375 28 350 1.11e-86 266
Msa0113340 MtrunA17_Chr4g0006991 39.826 344 188 8 51 375 84 427 1.60e-86 268
Msa0113340 MtrunA17_Chr4g0041011 40.181 331 176 7 53 375 47 363 1.62e-86 266
Msa0113340 MtrunA17_Chr4g0071391 34.908 381 221 6 21 375 67 446 1.14e-85 267
Msa0113340 MtrunA17_Chr1g0213281 36.243 378 215 8 23 375 139 515 2.49e-85 268
Msa0113340 MtrunA17_Chr5g0413271 38.323 334 197 4 51 375 143 476 8.10e-85 266
Msa0113340 MtrunA17_Chr3g0085081 40.233 343 179 7 40 373 21 346 2.71e-84 260
Msa0113340 MtrunA17_Chr4g0045951 40.000 335 184 5 47 375 39 362 5.20e-84 259
Msa0113340 MtrunA17_Chr7g0269991 37.126 334 188 6 53 371 137 463 2.82e-83 261
Msa0113340 MtrunA17_Chr3g0091031 39.942 343 180 7 40 373 21 346 3.05e-82 254
Msa0113340 MtrunA17_Chr8g0360521 40.816 343 179 9 53 375 71 409 8.09e-82 256
Msa0113340 MtrunA17_Chr3g0085071 38.554 332 185 5 49 373 42 361 6.31e-81 252
Msa0113340 MtrunA17_Chr4g0068111 37.135 342 194 6 53 375 114 453 7.02e-81 255
Msa0113340 MtrunA17_Chr4g0005491 37.037 351 198 9 48 375 57 407 6.78e-80 250
Msa0113340 MtrunA17_Chr3g0113671 37.647 340 191 7 53 371 106 445 7.21e-77 244
Msa0113340 MtrunA17_Chr7g0243301 34.336 399 226 11 10 375 14 409 9.43e-77 243
Msa0113340 MtrunA17_Chr7g0242701 38.841 345 192 7 50 375 83 427 2.72e-76 242
Msa0113340 MtrunA17_Chr8g0368461 36.544 353 194 7 52 375 177 528 1.00e-75 243
Msa0113340 MtrunA17_Chr5g0437291 37.681 345 191 8 51 371 163 507 5.98e-74 238
Msa0113340 MtrunA17_Chr3g0114101 35.673 342 201 7 53 375 89 430 1.74e-72 232
Msa0113340 MtrunA17_Chr5g0437601 35.447 347 205 7 48 375 78 424 5.72e-72 231
Msa0113340 MtrunA17_Chr2g0287901 35.511 352 197 8 48 375 92 437 4.38e-71 229
Msa0113340 MtrunA17_Chr7g0259711 35.311 354 206 6 43 375 81 432 6.95e-71 228
Msa0113340 MtrunA17_Chr4g0005481 35.244 349 206 8 45 375 100 446 1.63e-70 227
Msa0113340 MtrunA17_Chr3g0126021 35.072 345 196 7 49 375 125 459 2.48e-63 209
Msa0113340 MtrunA17_Chr8g0384151 32.853 347 207 7 44 375 81 416 1.13e-62 206
Msa0113340 MtrunA17_Chr1g0178081 31.658 398 192 12 1 375 1 341 1.51e-56 188
Msa0113340 MtrunA17_Chr1g0209921 39.485 233 129 7 50 276 41 267 1.52e-55 183
Msa0113340 MtrunA17_Chr8g0347321 28.686 373 225 13 36 375 75 439 1.85e-42 153
Msa0113340 MtrunA17_Chr6g0476041 30.667 375 207 15 40 375 154 514 7.40e-40 148
Msa0113340 MtrunA17_Chr1g0188191 30.585 376 211 15 36 375 220 581 2.34e-39 147
Msa0113340 MtrunA17_Chr8g0384421 30.027 373 217 14 35 375 112 472 7.29e-39 144
Msa0113340 MtrunA17_Chr7g0226991 28.125 352 224 9 50 375 54 402 1.48e-38 142
Msa0113340 MtrunA17_Chr5g0414051 28.451 355 213 10 53 375 75 420 1.48e-38 143
Msa0113340 MtrunA17_Chr4g0049421 28.329 353 215 13 53 375 48 392 2.10e-37 139
Msa0113340 MtrunA17_Chr8g0391481 28.333 360 219 11 47 375 21 372 2.66e-37 138
Msa0113340 MtrunA17_Chr3g0140371 26.575 365 230 7 40 375 5 360 2.67e-36 135
Msa0113340 MtrunA17_Chr4g0070341 30.084 359 212 15 45 375 124 471 8.42e-36 136
Msa0113340 MtrunA17_Chr5g0414061 29.428 367 211 15 44 375 85 438 1.36e-35 135
Msa0113340 MtrunA17_Chr8g0387921 26.316 361 219 13 50 375 71 419 1.06e-34 132
Msa0113340 MtrunA17_Chr5g0414091 28.852 357 220 12 48 375 80 431 1.89e-33 129
Msa0113340 MtrunA17_Chr2g0283851 29.282 362 193 14 57 375 59 400 7.70e-33 127
Msa0113340 MtrunA17_Chr6g0456681 28.969 359 221 11 45 375 56 408 9.92e-33 127
Msa0113340 MtrunA17_Chr6g0456691 27.200 375 227 11 36 375 56 419 2.82e-32 125
Msa0113340 MtrunA17_Chr6g0455901 27.750 400 238 16 8 371 28 412 4.34e-31 122
Msa0113340 MtrunA17_Chr5g0414041 30.435 368 208 15 44 375 78 433 5.20e-31 122
Msa0113340 MtrunA17_Chr6g0451321 28.610 367 203 17 48 375 111 457 3.21e-29 117
Msa0113340 MtrunA17_Chr7g0221291 45.833 96 51 1 49 143 75 170 1.53e-23 95.9
Msa0113340 MtrunA17_Chr7g0221271 36.301 146 85 4 38 178 63 205 6.16e-23 95.5
Msa0113340 MtrunA17_Chr3g0085101 36.522 115 63 2 238 344 1 113 2.08e-17 77.4
Msa0113340 MtrunA17_Chr1g0209931 40.000 105 44 3 275 375 3 92 1.80e-15 71.6
Msa0113340 MtrunA17_Chr2g0287931 32.773 119 68 2 112 218 2 120 2.17e-15 72.0
Msa0113340 MtrunA17_Chr5g0429191 50.000 44 22 0 325 368 2 45 5.65e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0113340 AT3G54260.1 62.368 380 132 6 1 375 1 374 4.25e-171 482
Msa0113340 AT2G31110.2 39.623 371 205 6 13 375 3 362 2.59e-92 281
Msa0113340 AT5G06700.1 41.159 345 186 6 48 375 250 594 2.68e-91 286
Msa0113340 AT5G58600.1 40.290 345 189 7 44 375 60 400 1.11e-90 278
Msa0113340 AT1G29050.1 39.714 350 185 5 41 375 40 378 1.13e-89 275
Msa0113340 AT2G30900.1 41.964 336 173 9 51 375 41 365 2.34e-88 271
Msa0113340 AT2G34070.1 38.576 337 189 5 46 375 58 383 1.42e-87 270
Msa0113340 AT2G42570.1 39.940 333 183 5 49 375 43 364 2.15e-87 269
Msa0113340 AT3G12060.1 39.710 345 191 5 48 375 191 535 8.46e-87 273
Msa0113340 AT5G20590.1 38.551 345 194 6 48 375 136 479 9.20e-86 268
Msa0113340 AT5G01360.2 38.439 346 191 8 51 375 34 378 1.15e-85 265
Msa0113340 AT5G01360.1 38.439 346 191 8 51 375 90 434 4.12e-85 265
Msa0113340 AT5G49340.1 38.398 362 202 8 35 375 80 441 2.80e-84 264
Msa0113340 AT3G62390.1 36.267 375 204 8 23 371 102 467 1.79e-83 262
Msa0113340 AT1G73140.1 40.058 342 183 9 53 375 67 405 3.74e-82 257
Msa0113340 AT2G30010.1 37.838 370 197 8 35 375 31 396 6.69e-82 256
Msa0113340 AT2G30010.2 37.838 370 197 8 35 375 74 439 1.60e-81 256
Msa0113340 AT2G40160.2 38.617 347 189 9 53 375 77 423 9.54e-81 254
Msa0113340 AT2G40160.1 38.329 347 190 9 53 375 77 423 2.11e-80 253
Msa0113340 AT1G60790.1 36.467 351 201 8 46 375 183 532 1.97e-79 253
Msa0113340 AT1G78710.1 34.897 341 203 7 43 375 28 357 2.71e-79 248
Msa0113340 AT3G14850.2 38.369 331 188 6 48 371 29 350 1.28e-78 246
Msa0113340 AT3G55990.1 37.719 342 192 7 51 371 138 479 9.55e-78 248
Msa0113340 AT2G40150.1 37.176 347 191 7 52 371 70 416 4.49e-77 244
Msa0113340 AT5G19160.1 36.559 372 200 9 32 375 81 444 8.89e-74 237
Msa0113340 AT2G38320.1 32.873 362 220 9 37 375 43 404 1.69e-73 234
Msa0113340 AT5G01620.2 34.293 382 228 10 13 375 72 449 4.08e-73 234
Msa0113340 AT5G01620.1 34.293 382 228 10 13 375 72 449 4.08e-73 234
Msa0113340 AT5G01620.3 34.293 382 228 10 13 375 80 457 5.53e-73 234
Msa0113340 AT3G11570.1 34.684 395 219 11 8 375 37 419 8.16e-72 231
Msa0113340 AT3G11030.1 35.838 346 196 8 53 375 106 448 2.02e-70 228
Msa0113340 AT3G06080.2 36.467 351 197 7 48 375 98 445 5.49e-70 227
Msa0113340 AT5G06230.2 37.815 357 181 10 50 375 18 364 9.52e-70 223
Msa0113340 AT2G40320.1 34.063 411 224 13 3 375 21 422 1.29e-69 225
Msa0113340 AT5G06230.1 37.815 357 181 10 50 375 59 405 1.86e-69 224
Msa0113340 AT1G48880.1 35.588 340 187 9 53 375 115 439 1.90e-67 220
Msa0113340 AT2G31110.3 40.230 261 151 4 13 270 3 261 2.24e-67 214
Msa0113340 AT2G30900.2 43.043 230 126 5 51 277 41 268 2.18e-64 206
Msa0113340 AT2G38320.2 33.022 321 194 7 76 375 1 321 3.69e-64 207
Msa0113340 AT5G58600.2 41.102 236 133 4 44 277 60 291 1.32e-63 205
Msa0113340 AT3G62390.2 36.765 272 155 4 23 277 102 373 1.12e-61 204
Msa0113340 AT2G40160.3 41.102 236 128 6 53 277 77 312 3.26e-60 197
Msa0113340 AT2G34070.2 37.308 260 146 4 122 375 1 249 7.65e-60 194
Msa0113340 AT5G20590.2 38.683 243 136 4 48 277 136 378 2.76e-56 189
Msa0113340 AT5G19160.2 37.066 259 147 4 32 277 81 336 9.41e-56 186
Msa0113340 AT1G78710.2 30.532 357 180 11 23 375 5 297 2.21e-53 179
Msa0113340 AT5G06230.3 38.057 247 134 4 50 277 59 305 6.03e-50 170
Msa0113340 AT3G06080.1 35.802 243 143 3 48 277 98 340 1.53e-49 170
Msa0113340 AT3G14850.1 36.328 256 148 5 122 371 1 247 5.01e-49 166
Msa0113340 AT2G31110.1 40.553 217 113 4 164 375 9 214 1.34e-48 164
Msa0113340 AT1G70230.1 32.486 354 187 15 53 375 79 411 1.12e-41 151
Msa0113340 AT4G25360.1 31.200 375 210 15 37 375 162 524 2.25e-41 152
Msa0113340 AT4G25360.2 31.200 375 210 15 37 375 162 524 2.25e-41 152
Msa0113340 AT1G01430.1 28.571 371 226 13 37 375 84 447 7.58e-41 150
Msa0113340 AT4G01080.1 29.060 351 210 13 49 371 87 426 1.97e-38 143
Msa0113340 AT5G51640.1 29.223 373 218 13 39 375 126 488 5.81e-38 142
Msa0113340 AT5G64020.1 28.492 358 215 14 53 375 57 408 2.37e-37 139
Msa0113340 AT4G11090.1 28.333 420 238 17 2 375 23 425 6.10e-37 139
Msa0113340 AT5G64470.3 27.095 358 228 12 43 375 45 394 1.52e-35 134
Msa0113340 AT5G64470.2 27.473 364 225 13 43 375 45 400 1.72e-35 134
Msa0113340 AT5G20680.6 27.202 386 225 18 26 375 92 457 2.21e-35 135
Msa0113340 AT2G37720.1 28.814 354 211 14 51 375 141 482 2.50e-35 135
Msa0113340 AT5G20680.2 27.202 386 225 18 26 375 168 533 5.19e-35 135
Msa0113340 AT4G23790.1 28.125 384 209 15 34 371 61 423 5.61e-35 133
Msa0113340 AT5G20680.4 27.202 386 225 18 26 375 186 551 6.35e-35 135
Msa0113340 AT5G20680.1 27.202 386 225 18 26 375 186 551 6.35e-35 135
Msa0113340 AT5G20680.3 27.202 386 225 18 26 375 186 551 6.35e-35 135
Msa0113340 AT5G20680.5 27.202 386 225 18 26 375 186 551 6.35e-35 135
Msa0113340 AT5G15900.1 28.926 363 224 11 40 375 54 409 2.14e-34 132
Msa0113340 AT5G15900.2 28.926 363 224 11 40 375 73 428 2.63e-34 132
Msa0113340 AT5G15890.1 28.691 359 209 11 47 375 181 522 4.19e-33 129
Msa0113340 AT3G28150.1 27.384 409 227 19 8 375 31 410 4.71e-32 125
Msa0113340 AT3G02440.1 27.273 352 217 12 53 375 127 468 7.66e-32 125
Msa0113340 AT3G02440.2 27.273 352 217 12 53 375 135 476 7.80e-32 125
Msa0113340 AT2G14530.1 27.424 361 214 13 50 375 59 406 8.19e-32 124
Msa0113340 AT5G64470.4 27.200 250 163 7 43 275 45 292 8.71e-24 100
Msa0113340 AT5G64470.1 27.200 250 163 7 43 275 45 292 1.17e-23 100

Find 131 sgRNAs with CRISPR-Local

Find 269 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCTGGTGTGCCTGATATT+TGG 0.189016 1_3:-29235132 Msa0113340:CDS
TGCTAGATTCTCTATTGTTT+TGG 0.195292 1_3:+29238008 None:intergenic
ATTTATATTTCTAGTCTAAA+TGG 0.198955 1_3:+29234583 None:intergenic
CTATTCTTTGTTTCTCATCT+TGG 0.240185 1_3:+29235185 None:intergenic
TAATACCAACTGGTAGAAAA+AGG 0.249328 1_3:-29236434 Msa0113340:CDS
CTTATGGTTGCTTATCAAAA+AGG 0.263528 1_3:-29235722 Msa0113340:CDS
CCACTGCACATTATCTCTTC+TGG 0.264239 1_3:-29238083 Msa0113340:CDS
TTTGATGCACTAAGGTTTCT+TGG 0.268531 1_3:-29236543 Msa0113340:CDS
CATCAAATATTGTCTTAAAA+AGG 0.270453 1_3:-29234640 Msa0113340:three_prime_UTR
GCTGAAGAAAATGAGATTTC+CGG 0.315862 1_3:-29235284 Msa0113340:CDS
TTCATCAAGGCATGATTTGC+GGG 0.320342 1_3:+29238247 None:intergenic
GTTACAATGCTGCCAACTTT+AGG 0.320362 1_3:+29234978 None:intergenic
GGTTCAATACCTCCTTTGTT+TGG 0.340933 1_3:-29234747 Msa0113340:three_prime_UTR
AAAATCATACTTATCCTATA+TGG 0.347597 1_3:+29234956 None:intergenic
ATTCCTCTGATTGCAGTCAT+TGG 0.349039 1_3:-29235159 Msa0113340:CDS
CTCTTCTGTGTAGGTGGAGG+GGG 0.358685 1_3:-29234719 Msa0113340:three_prime_UTR
TAGAATTTAATATCGTAATT+AGG 0.362576 1_3:-29235070 Msa0113340:three_prime_UTR
AACATCTCATTCCAAATATC+AGG 0.370214 1_3:+29235121 None:intergenic
TAGGGTTATGTAGAGTGAAA+AGG 0.375327 1_3:+29238202 None:intergenic
TGGACAATGTTAACATCATC+AGG 0.381677 1_3:+29238028 None:intergenic
GCATGTCACCTAGGCATAAC+AGG 0.386311 1_3:-29235631 Msa0113340:intron
GCAGAAAGTAGTGCCAGTTT+TGG 0.387798 1_3:-29234768 Msa0113340:three_prime_UTR
CACTTCTCATAATCAGAATC+TGG 0.390203 1_3:+29237875 None:intergenic
AGAGAAAGAAAATGAGTCAT+AGG 0.394269 1_3:+29238127 None:intergenic
AAACAAGAGAGTCTTACATT+TGG 0.395362 1_3:-29236228 Msa0113340:CDS
TTGGCGTTCTGCTGTGGTGC+TGG 0.405286 1_3:-29234806 Msa0113340:three_prime_UTR
AGGAGGTATTGAACCAAAAC+TGG 0.405538 1_3:+29234755 None:intergenic
TGAGTGAGTTAAGGAAGAGT+AGG 0.412926 1_3:+29238183 None:intergenic
ATTGAATGGAAGGCCATTGC+AGG 0.414061 1_3:+29236396 None:intergenic
AGGGTTATGTAGAGTGAAAA+GGG 0.414426 1_3:+29238203 None:intergenic
TGCAGTCATTGGTGCCTCCC+TGG 0.417455 1_3:-29235148 Msa0113340:CDS
CATGCTTCTGAAGATGACTT+TGG 0.432516 1_3:+29235649 None:intergenic
ATGTCTTCATTACCATTGAA+TGG 0.434469 1_3:+29236382 None:intergenic
TCATTGTCGTAATATCTTGC+AGG 0.436722 1_3:+29235257 None:intergenic
GAACTCTTCTGTGTAGGTGG+AGG 0.441370 1_3:-29234722 Msa0113340:three_prime_UTR
TGGAGCACTACTAAAGGTTC+AGG 0.445699 1_3:+29235313 None:intergenic
TCAATACCTCCTTTGTTTGG+TGG 0.448146 1_3:-29234744 Msa0113340:three_prime_UTR
GCAGTTACTTGTCAAAAGAA+TGG 0.450896 1_3:-29237902 Msa0113340:CDS
AACTCTTCTGTGTAGGTGGA+GGG 0.455050 1_3:-29234721 Msa0113340:three_prime_UTR
ATGGACATCCTTCAGTGTAT+AGG 0.463306 1_3:-29235219 Msa0113340:CDS
GAGTGAGTTAAGGAAGAGTA+GGG 0.463745 1_3:+29238184 None:intergenic
TGGCAGCATTGTAACCATAT+AGG 0.463752 1_3:-29234970 Msa0113340:three_prime_UTR
GACTAGAAATATAAATGAAA+AGG 0.466920 1_3:-29234576 Msa0113340:three_prime_UTR
GTAACTGCAGTACTAAGATA+AGG 0.467342 1_3:+29237917 None:intergenic
TTCCAAATATCAGGCACACC+AGG 0.469582 1_3:+29235130 None:intergenic
AGTGTGAAGTGAGTGAGTTA+AGG 0.469980 1_3:+29238174 None:intergenic
TCTGTAGGTTTGATGCACTA+AGG 0.472754 1_3:-29236551 Msa0113340:intron
GTTCAGGAGCATGTTGTTGA+AGG 0.476187 1_3:+29235329 None:intergenic
CAAGATGAGAAACAAAGAAT+AGG 0.477046 1_3:-29235184 Msa0113340:CDS
ACTCTTCTGTGTAGGTGGAG+GGG 0.477928 1_3:-29234720 Msa0113340:three_prime_UTR
CTTGGATTCTGCAACTTGGT+TGG 0.480932 1_3:-29234825 Msa0113340:three_prime_UTR
TTGACTAACAGTTGACTAAG+AGG 0.482312 1_3:+29235016 None:intergenic
TGCAATGGCCTTCCATTCAA+TGG 0.483402 1_3:-29236394 Msa0113340:intron
GTACTAAGATAAGGGCAGTT+AGG 0.483942 1_3:+29237926 None:intergenic
TTTCATCAAGGCATGATTTG+CGG 0.492463 1_3:+29238246 None:intergenic
CAGTTACTTGTCAAAAGAAT+GGG 0.497842 1_3:-29237901 Msa0113340:CDS
TTGGTGGAACTCTTCTGTGT+AGG 0.501360 1_3:-29234728 Msa0113340:three_prime_UTR
GTACAAGGTGTAATACCAAC+TGG 0.502784 1_3:-29236444 Msa0113340:CDS
GTATCTTGGATTCTGCAACT+TGG 0.509061 1_3:-29234829 Msa0113340:three_prime_UTR
TAACTGCAGTACTAAGATAA+GGG 0.511515 1_3:+29237918 None:intergenic
TCGAGGAGAATGCAAAGTAT+TGG 0.512927 1_3:-29236199 Msa0113340:CDS
CTGATTATGAGAAGTGGAAG+TGG 0.515175 1_3:-29237868 Msa0113340:CDS
GTAATATCTTGCAGGTATAC+CGG 0.515454 1_3:+29235265 None:intergenic
GAGGTGTCAAAGCCGCAGTC+TGG 0.518279 1_3:-29234857 Msa0113340:three_prime_UTR
TGTTGCCATGGAAGAAGCTA+AGG 0.519607 1_3:+29238150 None:intergenic
GAAAGCCAACATCATCTTGT+GGG 0.520387 1_3:+29238059 None:intergenic
CTTCATTACCATTGAATGGA+AGG 0.525435 1_3:+29236386 None:intergenic
TATTTGATTCAGCTCAGTGG+TGG 0.527529 1_3:-29236157 Msa0113340:CDS
CACTCCCTTAGCTTCTTCCA+TGG 0.528413 1_3:-29238155 Msa0113340:exon
TTCAATAATGAGAAATCAGT+GGG 0.529697 1_3:-29236484 Msa0113340:CDS
GGAAGTAAAGTGTTTCATCA+AGG 0.530205 1_3:+29238234 None:intergenic
CTTGGGATGGAGCAACCACT+TGG 0.534791 1_3:+29237842 None:intergenic
CTGGACCCACAAGATGATGT+TGG 0.536816 1_3:-29238064 Msa0113340:CDS
GTGGAACTCTTCTGTGTAGG+TGG 0.536904 1_3:-29234725 Msa0113340:three_prime_UTR
ACTTGGTTGGCGTTCTGCTG+TGG 0.538286 1_3:-29234812 Msa0113340:three_prime_UTR
TTCTTCAGTTCAACTAACAA+TGG 0.540448 1_3:+29236260 None:intergenic
ACTGAGTACATGGGCAAGGT+GGG 0.549939 1_3:-29235699 Msa0113340:CDS
GGGCAGTTAGGATCATAAAG+AGG 0.560599 1_3:+29237938 None:intergenic
AGGAAGAGAATCATGTTGGT+GGG 0.564160 1_3:-29236510 Msa0113340:CDS
AACTGTTAGTCAACATGTAA+CGG 0.565143 1_3:-29235006 Msa0113340:three_prime_UTR
ACTCCTTGGAGCACTACTAA+AGG 0.565747 1_3:+29235307 None:intergenic
ATGAGTCATAGGTGTTGCCA+TGG 0.568049 1_3:+29238138 None:intergenic
GTTCTGCTGTGGTGCTGGTG+TGG 0.569082 1_3:-29234801 Msa0113340:three_prime_UTR
GGAAGGCCATTGCAGGTCCA+TGG 0.570480 1_3:+29236403 None:intergenic
GGCAGTTAGGATCATAAAGA+GGG 0.572794 1_3:+29237939 None:intergenic
GACCTACCATGGACCTGCAA+TGG 0.573638 1_3:-29236409 Msa0113340:CDS
GAGAAGGAAGAGAATCATGT+TGG 0.573655 1_3:-29236514 Msa0113340:CDS
ATATAGTACCTGTTATGCCT+AGG 0.573860 1_3:+29235623 None:intergenic
CTTACATTTGGATTTGATCG+AGG 0.576704 1_3:-29236216 Msa0113340:CDS
GAGTACATGGGCAAGGTGGG+TGG 0.582998 1_3:-29235696 Msa0113340:CDS
TCTTTAGTATGCTTAGTACA+AGG 0.583313 1_3:-29236459 Msa0113340:CDS
ATGACTGCCTTACGCAGAGA+TGG 0.588976 1_3:-29235238 Msa0113340:CDS
GACTGAGTACATGGGCAAGG+TGG 0.591699 1_3:-29235700 Msa0113340:CDS
CAAGTGGTTGCTCCATCCCA+AGG 0.591851 1_3:-29237841 Msa0113340:intron
TTGTATTTGATTCAGCTCAG+TGG 0.592724 1_3:-29236160 Msa0113340:CDS
AGAGTGAAAAGGGTTGGTGT+TGG 0.593466 1_3:+29238213 None:intergenic
TTATGTAGAGTGAAAAGGGT+TGG 0.595226 1_3:+29238207 None:intergenic
AGAAAGCCAACATCATCTTG+TGG 0.596913 1_3:+29238058 None:intergenic
CAGATTCTGATTATGAGAAG+TGG 0.598360 1_3:-29237874 Msa0113340:CDS
GTAACGGAAGGACCTAAAGT+TGG 0.598411 1_3:-29234990 Msa0113340:three_prime_UTR
CTTGCCTTCCTATACACTGA+AGG 0.599517 1_3:+29235211 None:intergenic
AATGACTGCAATCAGAGGAA+TGG 0.604666 1_3:+29235161 None:intergenic
AGGATGTCCATCTCTGCGTA+AGG 0.610897 1_3:+29235231 None:intergenic
GGCCATTGCAGGTCCATGGT+AGG 0.611844 1_3:+29236407 None:intergenic
AAGGAAGAGAATCATGTTGG+TGG 0.613045 1_3:-29236511 Msa0113340:CDS
ATTCAATAATGAGAAATCAG+TGG 0.617219 1_3:-29236485 Msa0113340:CDS
AACTGATAATAGACTCACAT+GGG 0.624050 1_3:+29234673 None:intergenic
TCCAAATATCAGGCACACCA+GGG 0.626960 1_3:+29235131 None:intergenic
GCACCAATGACTGCAATCAG+AGG 0.627161 1_3:+29235156 None:intergenic
GAGAATGCAAAGTATTGGAG+AGG 0.631125 1_3:-29236194 Msa0113340:CDS
GAACCTTTAGTAGTGCTCCA+AGG 0.631877 1_3:-29235310 Msa0113340:CDS
ATACAGGTGGGATTACTACA+AGG 0.634100 1_3:-29235774 Msa0113340:intron
ACTGAGCTGAATCAAATACA+AGG 0.634640 1_3:+29236162 None:intergenic
TCTTCAGAAGCATGTCACCT+AGG 0.637548 1_3:-29235640 Msa0113340:CDS
TCATCAAGGCATGATTTGCG+GGG 0.644214 1_3:+29238248 None:intergenic
AGGTGGGATTACTACAAGGA+GGG 0.648410 1_3:-29235770 Msa0113340:CDS
AAGGACTGAGTACATGGGCA+AGG 0.650632 1_3:-29235703 Msa0113340:CDS
AGAAGAGTTCCACCAAACAA+AGG 0.651738 1_3:+29234735 None:intergenic
CCAGAAGAGATAATGTGCAG+TGG 0.657294 1_3:+29238083 None:intergenic
TTACTTGTCAAAAGAATGGG+AGG 0.658568 1_3:-29237898 Msa0113340:CDS
ACATCCTTCAGTGTATAGGA+AGG 0.659861 1_3:-29235215 Msa0113340:CDS
AGATGAACGTGAAATCAACA+TGG 0.663304 1_3:-29234905 Msa0113340:three_prime_UTR
GTTAGTCAACATGTAACGGA+AGG 0.664513 1_3:-29235002 Msa0113340:three_prime_UTR
GTTGCCATGGAAGAAGCTAA+GGG 0.675280 1_3:+29238151 None:intergenic
AGAGTTCCACCAAACAAAGG+AGG 0.691043 1_3:+29234738 None:intergenic
CAGGTGGGATTACTACAAGG+AGG 0.703135 1_3:-29235771 Msa0113340:CDS
AAACTGATAATAGACTCACA+TGG 0.704350 1_3:+29234672 None:intergenic
TGAACGTGAAATCAACATGG+AGG 0.717564 1_3:-29234902 Msa0113340:three_prime_UTR
AATGTGAACAGACCTTGGGA+TGG 0.722286 1_3:+29237829 None:intergenic
AAATATCAGGCACACCAGGG+AGG 0.749580 1_3:+29235134 None:intergenic
AAGTGGAAGTGGAAGCCAAG+TGG 0.762111 1_3:-29237857 Msa0113340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTAGATAAAATTTATATTT+TGG + chr1_3:29235078-29235097 None:intergenic 10.0%
!!! CAAAATATAAATTTTATCTA+AGG - chr1_3:29235076-29235095 Msa0113340:three_prime_UTR 10.0%
!!! AACTTTGTTTATCAATTAAA+TGG + chr1_3:29235224-29235243 None:intergenic 15.0%
!!! TAGTATATTCTAGATTTTTT+AGG - chr1_3:29235509-29235528 Msa0113340:intron 15.0%
!!! AGTATATTCTAGATTTTTTA+GGG - chr1_3:29235510-29235529 Msa0113340:intron 15.0%
!! AGTTCTTAAAATAAGTAAAA+TGG + chr1_3:29236003-29236022 None:intergenic 15.0%
!! CATTTACTTAAATACAAATT+TGG + chr1_3:29236086-29236105 None:intergenic 15.0%
!! TAGAATTTAATATCGTAATT+AGG - chr1_3:29237645-29237664 Msa0113340:intron 15.0%
!!! CTAATATACTTTTTGTAATT+AGG - chr1_3:29237675-29237694 Msa0113340:intron 15.0%
!!! ATTTATATTTCTAGTCTAAA+TGG + chr1_3:29238135-29238154 None:intergenic 15.0%
!!! AGATTTGATTTGACTTTAAA+TGG - chr1_3:29235353-29235372 Msa0113340:CDS 20.0%
!!! GATTTGATTTGACTTTAAAT+GGG - chr1_3:29235354-29235373 Msa0113340:CDS 20.0%
!!! TTTTTTTAGAACTCAATATC+CGG + chr1_3:29235432-29235451 None:intergenic 20.0%
!!! ATTGAGTTCTAAAAAAAACA+TGG - chr1_3:29235435-29235454 Msa0113340:intron 20.0%
!!! GTATATTCTAGATTTTTTAG+GGG - chr1_3:29235511-29235530 Msa0113340:intron 20.0%
!! AGATTTATCATTCAAATTTG+TGG - chr1_3:29235544-29235563 Msa0113340:intron 20.0%
!!! TTGTGTTATTGTAATTTGTA+CGG - chr1_3:29235722-29235741 Msa0113340:CDS 20.0%
!! CTTAAAATAAGTAAAATGGA+AGG + chr1_3:29235999-29236018 None:intergenic 20.0%
!!! TTCACTAAACTTTTTAAAAG+AGG - chr1_3:29236863-29236882 Msa0113340:intron 20.0%
!!! ATGACACCATAATATTTTAT+CGG - chr1_3:29237208-29237227 Msa0113340:intron 20.0%
!! TGATTTCCGATAAAATATTA+TGG + chr1_3:29237217-29237236 None:intergenic 20.0%
!!! TACAAAAAGTATATTAGTTC+AGG + chr1_3:29237672-29237691 None:intergenic 20.0%
!! AAAATCATACTTATCCTATA+TGG + chr1_3:29237762-29237781 None:intergenic 20.0%
!! CATCAAATATTGTCTTAAAA+AGG - chr1_3:29238075-29238094 Msa0113340:CDS 20.0%
!!! AGTCTAAATGGATTTTATAT+AGG + chr1_3:29238123-29238142 None:intergenic 20.0%
!! GACTAGAAATATAAATGAAA+AGG - chr1_3:29238139-29238158 Msa0113340:CDS 20.0%
! AGTGTGTAGAAAAAACAAAA+AGG + chr1_3:29234916-29234935 None:intergenic 25.0%
!! TTTTCTGTGCATAAAAAAGA+TGG + chr1_3:29235004-29235023 None:intergenic 25.0%
! TTTATCAATTAAATGGATGC+AGG + chr1_3:29235217-29235236 None:intergenic 25.0%
! GATCTCAAATCAATATCATT+AGG + chr1_3:29235263-29235282 None:intergenic 25.0%
!!! AGTTCTAAAAAAAACATGGT+TGG - chr1_3:29235439-29235458 Msa0113340:intron 25.0%
!!! TTTTTTATCAACTCTGAGTA+GGG - chr1_3:29235602-29235621 Msa0113340:intron 25.0%
!! TATTGTAATTTGTACGGATA+TGG - chr1_3:29235728-29235747 Msa0113340:CDS 25.0%
!! ACAAATATTTTCAGCCAAAA+TGG + chr1_3:29235919-29235938 None:intergenic 25.0%
!!! ATTTTGGCTGAAAATATTTG+TGG - chr1_3:29235918-29235937 Msa0113340:intron 25.0%
! TTTGTGGATTCTTATTAATG+TGG - chr1_3:29235934-29235953 Msa0113340:intron 25.0%
! AAAATAAGTAAAATGGAAGG+TGG + chr1_3:29235996-29236015 None:intergenic 25.0%
! GTTCTTGAAATTGTAAATGT+CGG + chr1_3:29236064-29236083 None:intergenic 25.0%
! GTAAATGAATTTCAACACTT+TGG - chr1_3:29236099-29236118 Msa0113340:intron 25.0%
! ATTCAATAATGAGAAATCAG+TGG - chr1_3:29236230-29236249 Msa0113340:CDS 25.0%
! TTCAATAATGAGAAATCAGT+GGG - chr1_3:29236231-29236250 Msa0113340:CDS 25.0%
! TGTCAATAATTTACAAAGGA+AGG + chr1_3:29236383-29236402 None:intergenic 25.0%
! GTGATGTCAATAATTTACAA+AGG + chr1_3:29236387-29236406 None:intergenic 25.0%
!! AGACATCAATTGAGTTTTTA+TGG - chr1_3:29236429-29236448 Msa0113340:CDS 25.0%
!! GACATCAATTGAGTTTTTAT+GGG - chr1_3:29236430-29236449 Msa0113340:CDS 25.0%
! AAACTATCTTAGAAAGCTTA+TGG - chr1_3:29236800-29236819 Msa0113340:intron 25.0%
! AACTATCTTAGAAAGCTTAT+GGG - chr1_3:29236801-29236820 Msa0113340:intron 25.0%
! GATAAAAAACATGACTCTTA+TGG - chr1_3:29236977-29236996 Msa0113340:intron 25.0%
!! GACAAAGATAAACAAGTTTT+AGG + chr1_3:29237131-29237150 None:intergenic 25.0%
! AATGCCAAAAACAAAATCAT+AGG + chr1_3:29237289-29237308 None:intergenic 25.0%
! AGGATTTCATTAGAAAAAGT+TGG - chr1_3:29237833-29237852 Msa0113340:intron 25.0%
! ATTTCATTAGAAAAAGTTGG+TGG - chr1_3:29237836-29237855 Msa0113340:intron 25.0%
! AAAAAGGAGATATATTTGTC+TGG - chr1_3:29238091-29238110 Msa0113340:CDS 25.0%
AGAGAAAGAAAATGAGTCAT+AGG + chr1_3:29234591-29234610 None:intergenic 30.0%
! TGCTAGATTCTCTATTGTTT+TGG + chr1_3:29234710-29234729 None:intergenic 30.0%
! TGACTTTTCTGTTGGAAAAT+GGG - chr1_3:29234737-29234756 Msa0113340:three_prime_UTR 30.0%
TAACTGCAGTACTAAGATAA+GGG + chr1_3:29234800-29234819 None:intergenic 30.0%
CAGTTACTTGTCAAAAGAAT+GGG - chr1_3:29234814-29234833 Msa0113340:three_prime_UTR 30.0%
GTGTAGAAAAAACAAAAAGG+TGG + chr1_3:29234913-29234932 None:intergenic 30.0%
!! TTGAAATTTTAAGTGCTCTG+AGG + chr1_3:29235187-29235206 None:intergenic 30.0%
GATACCTTCTGTATCAATTT+CGG - chr1_3:29235314-29235333 Msa0113340:CDS 30.0%
TAATCTCATTGTTCACTTGT+GGG + chr1_3:29235382-29235401 None:intergenic 30.0%
ATTAGATCTCTCGAATTAGT+CGG - chr1_3:29235398-29235417 Msa0113340:intron 30.0%
!!! GTTTTTTATCAACTCTGAGT+AGG - chr1_3:29235601-29235620 Msa0113340:intron 30.0%
! TTTTTATCAACTCTGAGTAG+GGG - chr1_3:29235603-29235622 Msa0113340:intron 30.0%
! TAAAATCTGAGATCAATGGA+GGG + chr1_3:29235662-29235681 None:intergenic 30.0%
! TTAAAATCTGAGATCAATGG+AGG + chr1_3:29235663-29235682 None:intergenic 30.0%
! GGATTAAAATCTGAGATCAA+TGG + chr1_3:29235666-29235685 None:intergenic 30.0%
AAGTTATGCATATAGGAGAT+GGG + chr1_3:29235687-29235706 None:intergenic 30.0%
ACTATGGAAGTTATGCATAT+AGG + chr1_3:29235694-29235713 None:intergenic 30.0%
TAACACAAAAACCATGACTA+TGG + chr1_3:29235710-29235729 None:intergenic 30.0%
GACTAACACTTACAATTTCA+GGG + chr1_3:29235803-29235822 None:intergenic 30.0%
TGACTAACACTTACAATTTC+AGG + chr1_3:29235804-29235823 None:intergenic 30.0%
! TTGCCTTTTCATGAAATTTG+GGG + chr1_3:29235834-29235853 None:intergenic 30.0%
! GTTGCCTTTTCATGAAATTT+GGG + chr1_3:29235835-29235854 None:intergenic 30.0%
! AGTTGCCTTTTCATGAAATT+TGG + chr1_3:29235836-29235855 None:intergenic 30.0%
TCTTTAGTATGCTTAGTACA+AGG - chr1_3:29236256-29236275 Msa0113340:CDS 30.0%
TAATACCAACTGGTAGAAAA+AGG - chr1_3:29236281-29236300 Msa0113340:CDS 30.0%
AATACCAACTGGTAGAAAAA+GGG - chr1_3:29236282-29236301 Msa0113340:CDS 30.0%
ATGTCTTCATTACCATTGAA+TGG + chr1_3:29236336-29236355 None:intergenic 30.0%
TTCTTCAGTTCAACTAACAA+TGG + chr1_3:29236458-29236477 None:intergenic 30.0%
AAACAAGAGAGTCTTACATT+TGG - chr1_3:29236487-29236506 Msa0113340:CDS 30.0%
ACGAGGGAAATTAAGAAAAT+GGG + chr1_3:29236656-29236675 None:intergenic 30.0%
TACGAGGGAAATTAAGAAAA+TGG + chr1_3:29236657-29236676 None:intergenic 30.0%
! ATTTTCTTAATTTCCCTCGT+AGG - chr1_3:29236656-29236675 Msa0113340:intron 30.0%
AATAAGCCAAAATCAGCTTA+CGG - chr1_3:29236824-29236843 Msa0113340:intron 30.0%
CTTATGGTTGCTTATCAAAA+AGG - chr1_3:29236993-29237012 Msa0113340:intron 30.0%
!!! AGATGACTTTGGTTTTTTGA+GGG + chr1_3:29237058-29237077 None:intergenic 30.0%
!!! AAGATGACTTTGGTTTTTTG+AGG + chr1_3:29237059-29237078 None:intergenic 30.0%
! ACTAAGTGAAGTTGATTACA+TGG - chr1_3:29237180-29237199 Msa0113340:intron 30.0%
! TGTTAAGATTTAGAGCTCAA+TGG + chr1_3:29237258-29237277 None:intergenic 30.0%
!!! TTCGCCTATGATTTTGTTTT+TGG - chr1_3:29237282-29237301 Msa0113340:intron 30.0%
! CTATTCTTTGTTTCTCATCT+TGG + chr1_3:29237533-29237552 None:intergenic 30.0%
CAAGATGAGAAACAAAGAAT+AGG - chr1_3:29237531-29237550 Msa0113340:intron 30.0%
AACATCTCATTCCAAATATC+AGG + chr1_3:29237597-29237616 None:intergenic 30.0%
AACTGTTAGTCAACATGTAA+CGG - chr1_3:29237709-29237728 Msa0113340:intron 30.0%
AACTGATAATAGACTCACAT+GGG + chr1_3:29238045-29238064 None:intergenic 30.0%
AAACTGATAATAGACTCACA+TGG + chr1_3:29238046-29238065 None:intergenic 30.0%
TTTCATCAAGGCATGATTTG+CGG + chr1_3:29234472-29234491 None:intergenic 35.0%
! GGAAGTAAAGTGTTTCATCA+AGG + chr1_3:29234484-29234503 None:intergenic 35.0%
TTATGTAGAGTGAAAAGGGT+TGG + chr1_3:29234511-29234530 None:intergenic 35.0%
AGGGTTATGTAGAGTGAAAA+GGG + chr1_3:29234515-29234534 None:intergenic 35.0%
TAGGGTTATGTAGAGTGAAA+AGG + chr1_3:29234516-29234535 None:intergenic 35.0%
TGGACAATGTTAACATCATC+AGG + chr1_3:29234690-29234709 None:intergenic 35.0%
! AAGAGATGTGACTTTTCTGT+TGG - chr1_3:29234729-29234748 Msa0113340:three_prime_UTR 35.0%
! GTGACTTTTCTGTTGGAAAA+TGG - chr1_3:29234736-29234755 Msa0113340:three_prime_UTR 35.0%
GTAACTGCAGTACTAAGATA+AGG + chr1_3:29234801-29234820 None:intergenic 35.0%
GCAGTTACTTGTCAAAAGAA+TGG - chr1_3:29234813-29234832 Msa0113340:three_prime_UTR 35.0%
TTACTTGTCAAAAGAATGGG+AGG - chr1_3:29234817-29234836 Msa0113340:three_prime_UTR 35.0%
CACTTCTCATAATCAGAATC+TGG + chr1_3:29234843-29234862 None:intergenic 35.0%
CAGATTCTGATTATGAGAAG+TGG - chr1_3:29234841-29234860 Msa0113340:three_prime_UTR 35.0%
! CTTAGAGTGTGCTTTTCTTA+AGG - chr1_3:29235282-29235301 Msa0113340:CDS 35.0%
AATCTCATTGTTCACTTGTG+GGG + chr1_3:29235381-29235400 None:intergenic 35.0%
CTAATCTCATTGTTCACTTG+TGG + chr1_3:29235383-29235402 None:intergenic 35.0%
TACTATGACTACTTGTGTCA+AGG + chr1_3:29235494-29235513 None:intergenic 35.0%
GAAGTTATGCATATAGGAGA+TGG + chr1_3:29235688-29235707 None:intergenic 35.0%
ATGCATAACTTCCATAGTCA+TGG - chr1_3:29235696-29235715 Msa0113340:CDS 35.0%
! GTGGATGATGAGTTTTTGAA+TGG - chr1_3:29235758-29235777 Msa0113340:CDS 35.0%
AGTCCCCAAATTTCATGAAA+AGG - chr1_3:29235828-29235847 Msa0113340:intron 35.0%
AGCTAGTTAAGTTTACAAGC+AGG + chr1_3:29236137-29236156 None:intergenic 35.0%
! AACTAGCTTATTACCTCTGT+AGG - chr1_3:29236149-29236168 Msa0113340:CDS 35.0%
TTTGATGCACTAAGGTTTCT+TGG - chr1_3:29236172-29236191 Msa0113340:CDS 35.0%
GGTTTCTTGGAAAAATGAGA+AGG - chr1_3:29236185-29236204 Msa0113340:CDS 35.0%
CTTCATTACCATTGAATGGA+AGG + chr1_3:29236332-29236351 None:intergenic 35.0%
! ATTGACATCACAGTTTTCTC+TGG - chr1_3:29236396-29236415 Msa0113340:CDS 35.0%
!!! TGTAAGACTCTCTTGTTTTC+TGG + chr1_3:29236485-29236504 None:intergenic 35.0%
CTTACATTTGGATTTGATCG+AGG - chr1_3:29236499-29236518 Msa0113340:CDS 35.0%
ACTGAGCTGAATCAAATACA+AGG + chr1_3:29236556-29236575 None:intergenic 35.0%
TTGTATTTGATTCAGCTCAG+TGG - chr1_3:29236555-29236574 Msa0113340:intron 35.0%
!!! TCCTTACGAGCTTGTTTTAT+CGG + chr1_3:29236591-29236610 None:intergenic 35.0%
AATGGGATGATATGAAGAGA+AGG + chr1_3:29236639-29236658 None:intergenic 35.0%
! TTAAGTAGTTTGTCCTACGA+GGG + chr1_3:29236672-29236691 None:intergenic 35.0%
! ATTAAGTAGTTTGTCCTACG+AGG + chr1_3:29236673-29236692 None:intergenic 35.0%
GCTTCTTTCTTGACTAATAC+AGG - chr1_3:29236925-29236944 Msa0113340:intron 35.0%
TCTTTCTTGACTAATACAGG+TGG - chr1_3:29236928-29236947 Msa0113340:intron 35.0%
CTTTCTTGACTAATACAGGT+GGG - chr1_3:29236929-29236948 Msa0113340:intron 35.0%
ATCAAAAAGGACTGAGTACA+TGG - chr1_3:29237006-29237025 Msa0113340:intron 35.0%
TCAAAAAGGACTGAGTACAT+GGG - chr1_3:29237007-29237026 Msa0113340:intron 35.0%
ATATAGTACCTGTTATGCCT+AGG + chr1_3:29237095-29237114 None:intergenic 35.0%
ACTTAGTGAGATGTAAGACT+TGG + chr1_3:29237167-29237186 None:intergenic 35.0%
! AAGATTTAGAGCTCAATGGT+TGG + chr1_3:29237254-29237273 None:intergenic 35.0%
!!! GGTTTTTTATGCAGGGAAAA+TGG - chr1_3:29237321-29237340 Msa0113340:intron 35.0%
TGTTGTTGAAGGCTGAAAAA+TGG + chr1_3:29237378-29237397 None:intergenic 35.0%
GCTGAAGAAAATGAGATTTC+CGG - chr1_3:29237431-29237450 Msa0113340:intron 35.0%
GTAATATCTTGCAGGTATAC+CGG + chr1_3:29237453-29237472 None:intergenic 35.0%
TCATTGTCGTAATATCTTGC+AGG + chr1_3:29237461-29237480 None:intergenic 35.0%
TTGACTAACAGTTGACTAAG+AGG + chr1_3:29237702-29237721 None:intergenic 35.0%
AGATGAACGTGAAATCAACA+TGG - chr1_3:29237810-29237829 Msa0113340:intron 35.0%
!! TCATTAGAAAAAGTTGGTGG+AGG - chr1_3:29237839-29237858 Msa0113340:intron 35.0%
AAGATACAAAAACCAGACTG+CGG + chr1_3:29237873-29237892 None:intergenic 35.0%
GAGTGAGTTAAGGAAGAGTA+GGG + chr1_3:29234534-29234553 None:intergenic 40.0%
TGAGTGAGTTAAGGAAGAGT+AGG + chr1_3:29234535-29234554 None:intergenic 40.0%
AGTGTGAAGTGAGTGAGTTA+AGG + chr1_3:29234544-29234563 None:intergenic 40.0%
GAAAGCCAACATCATCTTGT+GGG + chr1_3:29234659-29234678 None:intergenic 40.0%
AGAAAGCCAACATCATCTTG+TGG + chr1_3:29234660-29234679 None:intergenic 40.0%
GGCAGTTAGGATCATAAAGA+GGG + chr1_3:29234779-29234798 None:intergenic 40.0%
GTACTAAGATAAGGGCAGTT+AGG + chr1_3:29234792-29234811 None:intergenic 40.0%
CTGATTATGAGAAGTGGAAG+TGG - chr1_3:29234847-29234866 Msa0113340:three_prime_UTR 40.0%
ATGTTAACGTGTGCTCTTAG+TGG - chr1_3:29235038-29235057 Msa0113340:three_prime_UTR 40.0%
ACCTTCTGTATCAATTTCGG+TGG - chr1_3:29235317-29235336 Msa0113340:CDS 40.0%
TCCACCGAAATTGATACAGA+AGG + chr1_3:29235321-29235340 None:intergenic 40.0%
ATCTCATTGTTCACTTGTGG+GGG + chr1_3:29235380-29235399 None:intergenic 40.0%
TCTCGAATTAGTCGGTCTTT+AGG - chr1_3:29235406-29235425 Msa0113340:intron 40.0%
! ACAACTCACAAGGACCATTT+TGG - chr1_3:29235902-29235921 Msa0113340:intron 40.0%
TTAGTGCATCAAACCTACAG+AGG + chr1_3:29236165-29236184 None:intergenic 40.0%
TCTGTAGGTTTGATGCACTA+AGG - chr1_3:29236164-29236183 Msa0113340:CDS 40.0%
GAGAAGGAAGAGAATCATGT+TGG - chr1_3:29236201-29236220 Msa0113340:CDS 40.0%
AAGGAAGAGAATCATGTTGG+TGG - chr1_3:29236204-29236223 Msa0113340:CDS 40.0%
AGGAAGAGAATCATGTTGGT+GGG - chr1_3:29236205-29236224 Msa0113340:CDS 40.0%
GTACAAGGTGTAATACCAAC+TGG - chr1_3:29236271-29236290 Msa0113340:CDS 40.0%
! ACATCACAGTTTTCTCTGGT+AGG - chr1_3:29236400-29236419 Msa0113340:CDS 40.0%
TCGAGGAGAATGCAAAGTAT+TGG - chr1_3:29236516-29236535 Msa0113340:CDS 40.0%
!! GAGAATGCAAAGTATTGGAG+AGG - chr1_3:29236521-29236540 Msa0113340:CDS 40.0%
TATTTGATTCAGCTCAGTGG+TGG - chr1_3:29236558-29236577 Msa0113340:intron 40.0%
TCCGATAAAACAAGCTCGTA+AGG - chr1_3:29236587-29236606 Msa0113340:intron 40.0%
CAAAATCAGCTTACGGACAT+GGG - chr1_3:29236831-29236850 Msa0113340:intron 40.0%
ATACAGGTGGGATTACTACA+AGG - chr1_3:29236941-29236960 Msa0113340:intron 40.0%
! CATGCTTCTGAAGATGACTT+TGG + chr1_3:29237069-29237088 None:intergenic 40.0%
! TTAGAGCTCAATGGTTGGTT+TGG + chr1_3:29237249-29237268 None:intergenic 40.0%
! TTTTGGCATTCTCTAGCGAT+TGG - chr1_3:29237299-29237318 Msa0113340:intron 40.0%
TTTGGCATTCTCTAGCGATT+GGG - chr1_3:29237300-29237319 Msa0113340:intron 40.0%
!!! AGCGATTGGGTTTTTTATGC+AGG - chr1_3:29237313-29237332 Msa0113340:intron 40.0%
!!! GCGATTGGGTTTTTTATGCA+GGG - chr1_3:29237314-29237333 Msa0113340:intron 40.0%
TGAAGGCTGAAAAATGGTAG+AGG + chr1_3:29237372-29237391 None:intergenic 40.0%
! TCATTTTCTTCAGCACTCCT+TGG + chr1_3:29237425-29237444 None:intergenic 40.0%
ATGGACATCCTTCAGTGTAT+AGG - chr1_3:29237496-29237515 Msa0113340:intron 40.0%
ACATCCTTCAGTGTATAGGA+AGG - chr1_3:29237500-29237519 Msa0113340:intron 40.0%
AATGACTGCAATCAGAGGAA+TGG + chr1_3:29237557-29237576 None:intergenic 40.0%
ATTCCTCTGATTGCAGTCAT+TGG - chr1_3:29237556-29237575 Msa0113340:intron 40.0%
! GTTAGTCAACATGTAACGGA+AGG - chr1_3:29237713-29237732 Msa0113340:intron 40.0%
GTTACAATGCTGCCAACTTT+AGG + chr1_3:29237740-29237759 None:intergenic 40.0%
TGGCAGCATTGTAACCATAT+AGG - chr1_3:29237745-29237764 Msa0113340:intron 40.0%
TGAACGTGAAATCAACATGG+AGG - chr1_3:29237813-29237832 Msa0113340:intron 40.0%
!!! GCAGTCTGGTTTTTGTATCT+TGG - chr1_3:29237872-29237891 Msa0113340:CDS 40.0%
GTATCTTGGATTCTGCAACT+TGG - chr1_3:29237886-29237905 Msa0113340:CDS 40.0%
!! AGGAGGTATTGAACCAAAAC+TGG + chr1_3:29237963-29237982 None:intergenic 40.0%
GGTTCAATACCTCCTTTGTT+TGG - chr1_3:29237968-29237987 Msa0113340:CDS 40.0%
! TCAATACCTCCTTTGTTTGG+TGG - chr1_3:29237971-29237990 Msa0113340:CDS 40.0%
AGAAGAGTTCCACCAAACAA+AGG + chr1_3:29237983-29238002 None:intergenic 40.0%
!! AGAGTGAAAAGGGTTGGTGT+TGG + chr1_3:29234505-29234524 None:intergenic 45.0%
GTTGCCATGGAAGAAGCTAA+GGG + chr1_3:29234567-29234586 None:intergenic 45.0%
TGTTGCCATGGAAGAAGCTA+AGG + chr1_3:29234568-29234587 None:intergenic 45.0%
! ATGAGTCATAGGTGTTGCCA+TGG + chr1_3:29234580-29234599 None:intergenic 45.0%
CCAGAAGAGATAATGTGCAG+TGG + chr1_3:29234635-29234654 None:intergenic 45.0%
CCACTGCACATTATCTCTTC+TGG - chr1_3:29234632-29234651 Msa0113340:three_prime_UTR 45.0%
GGGCAGTTAGGATCATAAAG+AGG + chr1_3:29234780-29234799 None:intergenic 45.0%
AATGTGAACAGACCTTGGGA+TGG + chr1_3:29234889-29234908 None:intergenic 45.0%
TGGCAATGTGAACAGACCTT+GGG + chr1_3:29234893-29234912 None:intergenic 45.0%
GAATTAGTCGGTCTTTAGGC+CGG - chr1_3:29235410-29235429 Msa0113340:intron 45.0%
! ATCTGAGATCAATGGAGGGT+TGG + chr1_3:29235658-29235677 None:intergenic 45.0%
GTACGGATATGGTAAGCTTG+TGG - chr1_3:29235739-29235758 Msa0113340:CDS 45.0%
TTGCGAAGTCACAACTCACA+AGG - chr1_3:29235892-29235911 Msa0113340:intron 45.0%
! GTCACCCTTTTTCTACCAGT+TGG + chr1_3:29236289-29236308 None:intergenic 45.0%
AGAAAAAGGGTGACCTACCA+TGG - chr1_3:29236295-29236314 Msa0113340:CDS 45.0%
ATTGAATGGAAGGCCATTGC+AGG + chr1_3:29236322-29236341 None:intergenic 45.0%
TGCAATGGCCTTCCATTCAA+TGG - chr1_3:29236321-29236340 Msa0113340:intron 45.0%
GGGATGATATGAAGAGAAGG+TGG + chr1_3:29236636-29236655 None:intergenic 45.0%
!! CCATGTCCGTAAGCTGATTT+TGG + chr1_3:29236833-29236852 None:intergenic 45.0%
CCAAAATCAGCTTACGGACA+TGG - chr1_3:29236830-29236849 Msa0113340:intron 45.0%
AGGTGGGATTACTACAAGGA+GGG - chr1_3:29236945-29236964 Msa0113340:intron 45.0%
! TCTTCAGAAGCATGTCACCT+AGG - chr1_3:29237075-29237094 Msa0113340:intron 45.0%
! GTTCAGGAGCATGTTGTTGA+AGG + chr1_3:29237389-29237408 None:intergenic 45.0%
! TGGAGCACTACTAAAGGTTC+AGG + chr1_3:29237405-29237424 None:intergenic 45.0%
GAACCTTTAGTAGTGCTCCA+AGG - chr1_3:29237405-29237424 Msa0113340:intron 45.0%
! ACTCCTTGGAGCACTACTAA+AGG + chr1_3:29237411-29237430 None:intergenic 45.0%
CTTGCCTTCCTATACACTGA+AGG + chr1_3:29237507-29237526 None:intergenic 45.0%
TCCAAATATCAGGCACACCA+GGG + chr1_3:29237587-29237606 None:intergenic 45.0%
TTCCAAATATCAGGCACACC+AGG + chr1_3:29237588-29237607 None:intergenic 45.0%
! GTAACGGAAGGACCTAAAGT+TGG - chr1_3:29237725-29237744 Msa0113340:intron 45.0%
CTTGGATTCTGCAACTTGGT+TGG - chr1_3:29237890-29237909 Msa0113340:CDS 45.0%
! GCAGAAAGTAGTGCCAGTTT+TGG - chr1_3:29237947-29237966 Msa0113340:CDS 45.0%
AGAGTTCCACCAAACAAAGG+AGG + chr1_3:29237980-29237999 None:intergenic 45.0%
TTGGTGGAACTCTTCTGTGT+AGG - chr1_3:29237987-29238006 Msa0113340:CDS 45.0%
AACTCTTCTGTGTAGGTGGA+GGG - chr1_3:29237994-29238013 Msa0113340:CDS 45.0%
!!! TTTTTTTTTTAATTTTTAAC+AGG - chr1_3:29235158-29235177 Msa0113340:CDS 5.0%
CACTCCCTTAGCTTCTTCCA+TGG - chr1_3:29234560-29234579 Msa0113340:three_prime_UTR 50.0%
CTGGACCCACAAGATGATGT+TGG - chr1_3:29234651-29234670 Msa0113340:three_prime_UTR 50.0%
AAGTGGAAGTGGAAGCCAAG+TGG - chr1_3:29234858-29234877 Msa0113340:three_prime_UTR 50.0%
GTGGCAATGTGAACAGACCT+TGG + chr1_3:29234894-29234913 None:intergenic 50.0%
ATCAACTCTGAGTAGGGGAG+TGG - chr1_3:29235608-29235627 Msa0113340:intron 50.0%
TGAGTAGGGGAGTGGAATGT+GGG - chr1_3:29235616-29235635 Msa0113340:intron 50.0%
CAGGTGGGATTACTACAAGG+AGG - chr1_3:29236944-29236963 Msa0113340:intron 50.0%
AAGGACTGAGTACATGGGCA+AGG - chr1_3:29237012-29237031 Msa0113340:intron 50.0%
ACTGAGTACATGGGCAAGGT+GGG - chr1_3:29237016-29237035 Msa0113340:intron 50.0%
GCATGTCACCTAGGCATAAC+AGG - chr1_3:29237084-29237103 Msa0113340:intron 50.0%
ATGACTGCCTTACGCAGAGA+TGG - chr1_3:29237477-29237496 Msa0113340:intron 50.0%
AGGATGTCCATCTCTGCGTA+AGG + chr1_3:29237487-29237506 None:intergenic 50.0%
GCACCAATGACTGCAATCAG+AGG + chr1_3:29237562-29237581 None:intergenic 50.0%
AAATATCAGGCACACCAGGG+AGG + chr1_3:29237584-29237603 None:intergenic 50.0%
TCCCTGGTGTGCCTGATATT+TGG - chr1_3:29237583-29237602 Msa0113340:intron 50.0%
GTGGAACTCTTCTGTGTAGG+TGG - chr1_3:29237990-29238009 Msa0113340:CDS 50.0%
GAACTCTTCTGTGTAGGTGG+AGG - chr1_3:29237993-29238012 Msa0113340:CDS 50.0%
ACTCTTCTGTGTAGGTGGAG+GGG - chr1_3:29237995-29238014 Msa0113340:CDS 50.0%
CTTGGGATGGAGCAACCACT+TGG + chr1_3:29234876-29234895 None:intergenic 55.0%
CAAGTGGTTGCTCCATCCCA+AGG - chr1_3:29234874-29234893 Msa0113340:three_prime_UTR 55.0%
CTGAGTAGGGGAGTGGAATG+TGG - chr1_3:29235615-29235634 Msa0113340:intron 55.0%
GACCTACCATGGACCTGCAA+TGG - chr1_3:29236306-29236325 Msa0113340:intron 55.0%
GACTGAGTACATGGGCAAGG+TGG - chr1_3:29237015-29237034 Msa0113340:intron 55.0%
ACTTGGTTGGCGTTCTGCTG+TGG - chr1_3:29237903-29237922 Msa0113340:CDS 55.0%
CTCTTCTGTGTAGGTGGAGG+GGG - chr1_3:29237996-29238015 Msa0113340:CDS 55.0%
GGCCATTGCAGGTCCATGGT+AGG + chr1_3:29236311-29236330 None:intergenic 60.0%
GGAAGGCCATTGCAGGTCCA+TGG + chr1_3:29236315-29236334 None:intergenic 60.0%
GAGTACATGGGCAAGGTGGG+TGG - chr1_3:29237019-29237038 Msa0113340:intron 60.0%
! TGCAGTCATTGGTGCCTCCC+TGG - chr1_3:29237567-29237586 Msa0113340:intron 60.0%
! GAGGTGTCAAAGCCGCAGTC+TGG - chr1_3:29237858-29237877 Msa0113340:CDS 60.0%
TTGGCGTTCTGCTGTGGTGC+TGG - chr1_3:29237909-29237928 Msa0113340:CDS 60.0%
!! GTTCTGCTGTGGTGCTGGTG+TGG - chr1_3:29237914-29237933 Msa0113340:CDS 60.0%
TGTGTAGGTGGAGGGGGCTT+TGG - chr1_3:29238002-29238021 Msa0113340:CDS 60.0%
! GTGGAGGGGGCTTTGGTGTG+TGG - chr1_3:29238009-29238028 Msa0113340:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 29234469 29238268 29234469 ID=Msa0113340;Name=Msa0113340
chr1_3 mRNA 29234469 29238268 29234469 ID=Msa0113340-mRNA-1;Parent=Msa0113340;Name=Msa0113340-mRNA-1;_AED=0.11;_eAED=0.11;_QI=110|1|1|1|1|1|5|636|375
chr1_3 exon 29234469 29235402 29234469 ID=Msa0113340-mRNA-1:exon:9671;Parent=Msa0113340-mRNA-1
chr1_3 exon 29235632 29235790 29235632 ID=Msa0113340-mRNA-1:exon:9670;Parent=Msa0113340-mRNA-1
chr1_3 exon 29236134 29236315 29236134 ID=Msa0113340-mRNA-1:exon:9669;Parent=Msa0113340-mRNA-1
chr1_3 exon 29236395 29236566 29236395 ID=Msa0113340-mRNA-1:exon:9668;Parent=Msa0113340-mRNA-1
chr1_3 exon 29237842 29238268 29237842 ID=Msa0113340-mRNA-1:exon:9667;Parent=Msa0113340-mRNA-1
chr1_3 five_prime_UTR 29238159 29238268 29238159 ID=Msa0113340-mRNA-1:five_prime_utr;Parent=Msa0113340-mRNA-1
chr1_3 CDS 29237842 29238158 29237842 ID=Msa0113340-mRNA-1:cds;Parent=Msa0113340-mRNA-1
chr1_3 CDS 29236395 29236566 29236395 ID=Msa0113340-mRNA-1:cds;Parent=Msa0113340-mRNA-1
chr1_3 CDS 29236134 29236315 29236134 ID=Msa0113340-mRNA-1:cds;Parent=Msa0113340-mRNA-1
chr1_3 CDS 29235632 29235790 29235632 ID=Msa0113340-mRNA-1:cds;Parent=Msa0113340-mRNA-1
chr1_3 CDS 29235105 29235402 29235105 ID=Msa0113340-mRNA-1:cds;Parent=Msa0113340-mRNA-1
chr1_3 three_prime_UTR 29234469 29235104 29234469 ID=Msa0113340-mRNA-1:three_prime_utr;Parent=Msa0113340-mRNA-1
Gene Sequence

>Msa0113340

ATGGCAACACCTATGACTCATTTTCTTTCTCTTTTCTTGTTGTTCAACTTCTTCCACTGCACATTATCTCTTCTGGACCCACAAGATGATGTTGGCTTTCTAGTTGAACCTGATGATGTTAACATTGTCCAAAACAATAGAGAATCTAGCAAGAGATGTGACTTTTCTGTTGGAAAATGGGTTTATGATGAATCTTACCCTCTTTATGATCCTAACTGCCCTTATCTTAGTACTGCAGTTACTTGTCAAAAGAATGGGAGGCCAGATTCTGATTATGAGAAGTGGAAGTGGAAGCCAAGTGGTTGCTCCATCCCAAGGTTTGATGCACTAAGGTTTCTTGGAAAAATGAGAAGGAAGAGAATCATGTTGGTGGGTGATTCAATAATGAGAAATCAGTGGGAATCTTTAGTATGCTTAGTACAAGGTGTAATACCAACTGGTAGAAAAAGGGTGACCTACCATGGACCTGCAATGGCCTTCCATTCAATGGATTTTGAGACATCAATTGAGTTTTTATGGGCACCATTGTTAGTTGAACTGAAGAAAAGTCCAGAAAACAAGAGAGTCTTACATTTGGATTTGATCGAGGAGAATGCAAAGTATTGGAGAGGAGTTGATATCCTTGTATTTGATTCAGCTCAGTGGTGGACTCACTCCGATAAAACAAGCTCGTGGGATTACTACAAGGAGGGAAATAATCTGATAAAAAACATGACTCTTATGGTTGCTTATCAAAAAGGACTGAGTACATGGGCAAGGTGGGTGGATCTAAATCTAAACCCTCAAAAAACCAAAGTCATCTTCAGAAGCATGTCACCTAGGCATAACAGGGAAAATGGTTCGAAATGCGACAATCAGAAACAACCTCTACCATTTTTCAGCCTTCAACAACATGCTCCTGAACCTTTAGTAGTGCTCCAAGGAGTGCTGAAGAAAATGAGATTTCCGGTATACCTGCAAGATATTACGACAATGACTGCCTTACGCAGAGATGGACATCCTTCAGTGTATAGGAAGGCAAGCAGCCAAGATGAGAAACAAAGAATAGGCCATTCCTCTGATTGCAGTCATTGGTGCCTCCCTGGTGTGCCTGATATTTGGAATGAGATGTTGAGTGCGCTGCTTTGA
Protein sequence

>Msa0113340

MATPMTHFLSLFLLFNFFHCTLSLLDPQDDVGFLVEPDDVNIVQNNRESSKRCDFSVGKWVYDESYPLYDPNCPYLSTAVTCQKNGRPDSDYEKWKWKPSGCSIPRFDALRFLGKMRRKRIMLVGDSIMRNQWESLVCLVQGVIPTGRKRVTYHGPAMAFHSMDFETSIEFLWAPLLVELKKSPENKRVLHLDLIEENAKYWRGVDILVFDSAQWWTHSDKTSSWDYYKEGNNLIKNMTLMVAYQKGLSTWARWVDLNLNPQKTKVIFRSMSPRHNRENGSKCDNQKQPLPFFSLQQHAPEPLVVLQGVLKKMRFPVYLQDITTMTALRRDGHPSVYRKASSQDEKQRIGHSSDCSHWCLPGVPDIWNEMLSALL*