AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1383310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1383310 MtrunA17_Chr3g0135851 98.652 445 6 0 1 445 1 445 0.0 907
Msa1383310 MtrunA17_Chr8g0342551 44.091 440 218 10 1 432 1 420 8.49e-76 243
Msa1383310 MtrunA17_Chr2g0330051 44.548 431 189 13 1 427 1 385 3.84e-73 235
Msa1383310 MtrunA17_Chr4g0025441 47.122 278 124 6 1 268 1 265 4.95e-58 197
Msa1383310 MtrunA17_Chr6g0462191 39.016 305 151 11 89 380 105 387 2.84e-42 154
Msa1383310 MtrunA17_Chr1g0200161 43.503 177 90 4 90 263 140 309 1.35e-37 143
Msa1383310 MtrunA17_Chr8g0346021 44.041 193 80 5 89 265 125 305 1.11e-36 141
Msa1383310 MtrunA17_Chr7g0263731 42.478 226 106 7 50 265 64 275 2.27e-35 136
Msa1383310 MtrunA17_Chr1g0181581 44.503 191 86 6 72 259 103 276 8.30e-35 135
Msa1383310 MtrunA17_Chr2g0283321 35.600 250 122 8 93 332 65 285 1.07e-32 125
Msa1383310 MtrunA17_Chr2g0316401 35.514 214 127 6 58 270 42 245 4.83e-32 124
Msa1383310 MtrunA17_Chr4g0045641 30.114 352 207 12 56 386 112 445 1.77e-30 123
Msa1383310 MtrunA17_Chr4g0020671 31.466 232 131 5 50 276 60 268 1.38e-28 117
Msa1383310 MtrunA17_Chr1g0146931 39.604 202 101 3 86 267 117 317 1.12e-25 109
Msa1383310 MtrunA17_Chr1g0204901 49.524 105 51 1 90 192 135 239 3.63e-24 104
Msa1383310 MtrunA17_Chr7g0273861 29.947 187 108 3 86 268 120 287 2.49e-21 95.9
Msa1383310 MtrunA17_Chr8g0340761 62.162 74 28 0 85 158 120 193 3.75e-21 95.1
Msa1383310 MtrunA17_Chr3g0126901 38.947 190 108 4 91 279 112 294 6.26e-21 94.7
Msa1383310 MtrunA17_Chr8g0371131 40.000 140 74 3 28 159 2 139 9.95e-21 88.2
Msa1383310 MtrunA17_Chr6g0472551 56.098 82 36 0 86 167 112 193 9.17e-20 91.3
Msa1383310 MtrunA17_Chr8g0389781 63.636 66 24 0 90 155 125 190 1.18e-19 90.9
Msa1383310 MtrunA17_Chr2g0330921 36.628 172 89 5 1 159 1 165 1.43e-18 86.7
Msa1383310 MtrunA17_Chr1g0203711 58.571 70 29 0 90 159 114 183 1.55e-18 87.4
Msa1383310 MtrunA17_Chr4g0060381 33.158 190 97 5 74 262 83 243 4.43e-18 86.7
Msa1383310 MtrunA17_Chr4g0057321 33.511 188 99 6 1 180 1 170 1.22e-17 84.7
Msa1383310 MtrunA17_Chr7g0273951 56.716 67 29 0 93 159 108 174 1.60e-17 84.0
Msa1383310 MtrunA17_Chr1g0166431 47.222 108 52 1 90 192 116 223 4.24e-17 83.2
Msa1383310 MtrunA17_Chr5g0416161 36.290 124 63 3 69 191 99 207 5.59e-13 71.2
Msa1383310 MtrunA17_Chr1g0172611 32.847 137 81 2 93 220 107 241 5.96e-13 70.1
Msa1383310 MtrunA17_Chr1g0156121 49.254 67 34 0 91 157 124 190 1.06e-12 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1383310 AT2G26180.1 59.502 442 147 12 1 439 1 413 3.92e-149 431
Msa1383310 AT1G72670.1 45.618 445 189 14 1 436 1 401 1.96e-94 291
Msa1383310 AT1G17480.1 37.277 448 196 14 1 440 1 371 1.66e-65 215
Msa1383310 AT3G22190.3 45.161 279 122 8 1 268 1 259 4.43e-49 173
Msa1383310 AT3G22190.2 45.161 279 122 8 1 268 1 259 4.43e-49 173
Msa1383310 AT3G22190.4 45.161 279 122 8 1 268 1 259 4.43e-49 173
Msa1383310 AT3G22190.1 45.161 279 122 8 1 268 1 259 4.43e-49 173
Msa1383310 AT5G03040.1 43.627 204 105 4 71 272 94 289 4.07e-41 152
Msa1383310 AT5G03040.3 43.627 204 105 4 71 272 94 289 4.07e-41 152
Msa1383310 AT5G03040.2 43.627 204 105 4 71 272 94 289 4.07e-41 152
Msa1383310 AT4G00820.1 33.668 398 180 15 89 443 130 486 1.42e-37 144
Msa1383310 AT1G01110.2 31.159 414 228 12 14 385 44 442 2.29e-36 140
Msa1383310 AT3G52290.1 39.674 184 102 2 90 273 107 281 3.91e-35 135
Msa1383310 AT3G15050.1 38.496 226 110 8 91 315 50 247 1.02e-32 125
Msa1383310 AT3G09710.1 38.839 224 108 7 64 269 67 279 1.35e-32 129
Msa1383310 AT3G49260.3 40.513 195 93 4 86 265 115 301 1.94e-32 129
Msa1383310 AT3G49260.1 40.000 195 93 4 86 265 115 300 2.20e-32 128
Msa1383310 AT3G49260.4 40.000 195 93 4 86 265 115 300 2.20e-32 128
Msa1383310 AT3G49260.2 40.000 195 93 4 86 265 115 300 2.20e-32 128
Msa1383310 AT3G09710.2 36.709 237 109 8 64 269 67 293 4.05e-31 125
Msa1383310 AT2G33990.1 37.853 177 99 4 90 265 46 212 1.21e-29 116
Msa1383310 AT4G10640.1 31.864 295 154 9 15 278 22 300 4.70e-27 112
Msa1383310 AT2G26410.2 35.028 177 106 4 89 263 146 315 1.84e-25 109
Msa1383310 AT2G26410.1 35.028 177 106 4 89 263 146 315 1.84e-25 109
Msa1383310 AT5G13460.1 31.897 232 120 8 58 284 86 284 6.25e-23 101
Msa1383310 AT2G43680.4 32.402 179 102 5 77 255 311 470 1.15e-21 98.6
Msa1383310 AT2G43680.2 32.402 179 102 5 77 255 311 470 1.15e-21 98.6
Msa1383310 AT2G43680.3 32.402 179 102 5 77 255 311 470 1.15e-21 98.6
Msa1383310 AT2G43680.5 32.402 179 102 5 77 255 311 470 1.15e-21 98.6
Msa1383310 AT2G43680.1 32.402 179 102 5 77 255 312 471 1.25e-21 98.2
Msa1383310 AT4G14750.1 30.802 237 143 7 1 221 1 232 1.98e-21 95.9
Msa1383310 AT4G14750.3 30.802 237 143 7 1 221 60 291 3.71e-21 95.9
Msa1383310 AT4G14750.2 30.802 237 143 7 1 221 60 291 4.44e-21 95.5
Msa1383310 AT3G59690.2 34.641 153 92 4 81 231 160 306 7.97e-21 95.5
Msa1383310 AT3G59690.1 34.641 153 92 4 81 231 160 306 7.97e-21 95.5
Msa1383310 AT1G51960.1 33.173 208 117 7 1 198 1 196 8.00e-19 87.8
Msa1383310 AT4G23060.1 57.333 75 32 0 91 165 167 241 2.02e-18 87.8
Msa1383310 AT3G16490.1 49.451 91 38 2 90 180 106 188 2.86e-18 86.7
Msa1383310 AT1G18840.1 48.052 77 40 0 88 164 106 182 7.35e-18 86.7
Msa1383310 AT1G18840.3 48.052 77 40 0 88 164 106 182 7.35e-18 86.7
Msa1383310 AT1G18840.2 48.052 77 40 0 88 164 106 182 7.35e-18 86.7
Msa1383310 AT5G07240.1 66.667 63 20 1 90 151 104 166 2.49e-17 84.0
Msa1383310 AT5G07240.2 66.667 63 20 1 90 151 150 212 2.89e-17 84.3
Msa1383310 AT3G51380.1 57.812 64 27 0 89 152 35 98 8.47e-16 73.6
Msa1383310 AT1G14380.6 35.928 167 90 5 1 157 1 160 1.13e-15 79.7
Msa1383310 AT1G14380.5 35.928 167 90 5 1 157 1 160 1.13e-15 79.7
Msa1383310 AT1G14380.2 35.928 167 90 5 1 157 1 160 1.13e-15 79.7
Msa1383310 AT1G14380.7 35.928 167 90 5 1 157 1 160 1.22e-15 79.7
Msa1383310 AT1G74690.1 39.316 117 66 1 62 178 84 195 1.27e-15 79.7
Msa1383310 AT4G29150.1 61.538 65 25 0 89 153 129 193 1.28e-15 79.0
Msa1383310 AT1G14380.1 35.928 167 90 5 1 157 1 160 1.32e-15 79.7
Msa1383310 AT1G14380.3 35.928 167 90 5 1 157 1 160 1.32e-15 79.7
Msa1383310 AT1G14380.4 35.928 167 90 5 1 157 1 160 1.32e-15 79.7
Msa1383310 AT5G03960.3 37.956 137 74 2 93 222 110 242 7.04e-15 76.6
Msa1383310 AT5G03960.2 37.956 137 74 2 93 222 110 242 7.04e-15 76.6
Msa1383310 AT5G03960.1 37.956 137 74 2 93 222 109 241 7.33e-15 76.6
Msa1383310 AT1G01110.1 28.125 288 162 9 134 385 1 279 1.28e-14 75.5
Msa1383310 AT5G62070.1 61.667 60 23 0 86 145 112 171 2.21e-14 75.1
Msa1383310 AT3G49380.1 38.235 136 67 4 19 147 41 166 3.23e-14 74.3
Msa1383310 AT1G19870.1 35.938 128 60 3 47 152 149 276 4.33e-13 72.0
Msa1383310 AT1G19870.2 53.226 62 29 0 91 152 32 93 1.89e-12 69.7
Msa1383310 AT2G02790.2 41.892 74 43 0 84 157 100 173 9.81e-11 64.3
Msa1383310 AT2G02790.3 41.892 74 43 0 84 157 100 173 9.81e-11 64.3

Find 166 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGACGAATTGTCAATTTA+TGG 0.120322 tig0020594:+3627 Msa1383310:three_prime_UTR
TCATGGGTGCTTCTGGAAAA+TGG 0.162318 tig0020594:+1246 Msa1383310:exon
TTATTTCTAATAGTAATTTA+TGG 0.163021 tig0020594:+3824 Msa1383310:three_prime_UTR
TGTTATACAACATGATTATT+TGG 0.179043 tig0020594:+3783 Msa1383310:three_prime_UTR
CAACAAGATGTTGAACTTCT+TGG 0.237700 tig0020594:-3460 None:intergenic
AACGAGTAGCTTGTCCAATA+TGG 0.247199 tig0020594:-3017 None:intergenic
CGGTGAGACCATGGTGAAAA+AGG 0.254663 tig0020594:+3388 Msa1383310:exon
AAGGGTTCTGCAAATTTCTT+TGG 0.258449 tig0020594:-2913 None:intergenic
GTTTCGGCATTCACCTGATA+TGG 0.260918 tig0020594:-4169 Msa1383320:CDS
TGAGTCAGGTTCATGTAATT+TGG 0.262145 tig0020594:-3168 None:intergenic
GCAACACTCATGGGTGCTTC+TGG 0.264318 tig0020594:+1239 Msa1383310:exon
TCTAAAGGGCTTTCCATATC+AGG 0.269204 tig0020594:+4156 Msa1383310:three_prime_UTR
AACCACTTTCCGCGCCTTCT+TGG 0.292272 tig0020594:+1624 Msa1383310:CDS
CCATGAGTGTTGCTGAGTTT+TGG 0.299452 tig0020594:-1229 None:intergenic
CTTTGTTCATCTCGTGATTC+CGG 0.304293 tig0020594:-2780 None:intergenic
CCTTATGCTTTCATACCATT+TGG 0.309982 tig0020594:-4276 Msa1383320:CDS
AGCAAGCTGCAGTGACATTA+AGG 0.326388 tig0020594:+2060 Msa1383310:CDS
TCTCACCATTAGATGATATA+TGG 0.337909 tig0020594:+3916 Msa1383310:three_prime_UTR
CTCAACCTGGAGAGATTATC+TGG 0.341877 tig0020594:+3608 Msa1383310:three_prime_UTR
TGGACGAATTGTCAATTTAT+GGG 0.347242 tig0020594:+3628 Msa1383310:three_prime_UTR
TGTCTTGTGCATCATAGAAT+TGG 0.353917 tig0020594:-3847 None:intergenic
AGCCCTTTAGAACTCGAATA+TGG 0.355019 tig0020594:-4144 Msa1383320:CDS
TATACAGAAAATACTTGTTT+CGG 0.356055 tig0020594:-4185 Msa1383320:CDS
GCTAAGCCTCCTCCCCATAT+TGG 0.363494 tig0020594:+3003 Msa1383310:CDS
AACCAAGGCAAAGCTAAAAT+CGG 0.368576 tig0020594:+3194 Msa1383310:CDS
AATTGTTCATTTGTGAGTAT+TGG 0.372492 tig0020594:+3580 Msa1383310:three_prime_UTR
TTGCCTTGGTTGATTGAGTC+AGG 0.373865 tig0020594:-3182 None:intergenic
GGTGAGATTTATTGATTCAA+TGG 0.373983 tig0020594:-3900 None:intergenic
ACATGTTGTTCATCTTTGTC+TGG 0.374417 tig0020594:-1296 None:intergenic
AATTTCTTTGGAGGTGGCGG+TGG 0.375706 tig0020594:-2901 None:intergenic
GCTTTCATACCATTTGGAAT+TGG 0.378600 tig0020594:-4270 Msa1383320:CDS
AGAGAAAGAGAAGGGTTCGT+GGG 0.379570 tig0020594:-1102 None:intergenic
CCAAATGGTATGAAAGCATA+AGG 0.390674 tig0020594:+4276 Msa1383310:three_prime_UTR
CACCGAGCTCTTGTCCGATT+GGG 0.391424 tig0020594:-3370 None:intergenic
TGAGGATTCAAGCACTTGTC+AGG 0.392686 tig0020594:+2021 Msa1383310:CDS
CACGAGATGAACAAAGCTAA+TGG 0.399444 tig0020594:+2787 Msa1383310:CDS
AGAAGGCGCGGAAAGTGGTT+TGG 0.400404 tig0020594:-1621 None:intergenic
ACGAGATGAACAAAGCTAAT+GGG 0.406154 tig0020594:+2788 Msa1383310:CDS
CAAGTCCAAGTTCCGAGTTT+CGG 0.408556 tig0020594:+3046 Msa1383310:CDS
ACCCTTCAGAAGCTGCTTTG+TGG 0.408756 tig0020594:-1489 None:intergenic
GCCTTGGGAGACTAGATTGA+TGG 0.409779 tig0020594:+2846 Msa1383310:CDS
GATGGAGTTGGTTGGAAAGA+TGG 0.411885 tig0020594:+2812 Msa1383310:CDS
CTAACAAGTCTAAAATCCTT+AGG 0.416040 tig0020594:-1574 None:intergenic
TTCCATATTCGAGTTCTAAA+GGG 0.417925 tig0020594:+4142 Msa1383310:three_prime_UTR
TGAAAGCTTGATCTCTTGTA+TGG 0.419879 tig0020594:+4218 Msa1383310:three_prime_UTR
ATTCCATATTCGAGTTCTAA+AGG 0.422142 tig0020594:+4141 Msa1383310:three_prime_UTR
TCGTACCGAAACTCGGAACT+TGG 0.431873 tig0020594:-3051 None:intergenic
TCACCGAGCTCTTGTCCGAT+TGG 0.433938 tig0020594:-3371 None:intergenic
CATGGGTGCTTCTGGAAAAT+GGG 0.436014 tig0020594:+1247 Msa1383310:exon
AAATGGGTCAAAGCATTAAT+CGG 0.439295 tig0020594:+1263 Msa1383310:CDS
GAGAGAAAGAGAAGGGTTCG+TGG 0.444055 tig0020594:-1103 None:intergenic
CAAATGAACAATTATACAAA+TGG 0.444321 tig0020594:-3570 None:intergenic
AAGGGCTTTGAGGGCATTGA+AGG 0.446119 tig0020594:+1992 Msa1383310:CDS
ATTTCTTTGGAGGTGGCGGT+GGG 0.446397 tig0020594:-2900 None:intergenic
TCACACCATATATCATCTAA+TGG 0.448162 tig0020594:-3921 None:intergenic
ACGAGTAGCTTGTCCAATAT+GGG 0.450987 tig0020594:-3016 None:intergenic
TTAGACTTGTTAGGCAAGAA+TGG 0.452196 tig0020594:+1584 Msa1383310:CDS
AGCTCAAAGGCAACAGTCAA+TGG 0.457003 tig0020594:+3239 Msa1383310:CDS
TAGACTTGTTAGGCAAGAAT+GGG 0.462512 tig0020594:+1585 Msa1383310:CDS
GGTTCTGCAAATTTCTTTGG+AGG 0.465140 tig0020594:-2910 None:intergenic
TGCAGGCAAGAAGGGCTTTG+AGG 0.465225 tig0020594:+1982 Msa1383310:intron
TTTGAGATTTCCAGCAATGC+AGG 0.466329 tig0020594:+2710 Msa1383310:intron
GATGGATGGCAGCCAAGCCT+TGG 0.468070 tig0020594:+2830 Msa1383310:CDS
CATCAGGAGATAACAGTTCA+TGG 0.470128 tig0020594:+1446 Msa1383310:CDS
GCAGGCAAGAAGGGCTTTGA+GGG 0.472599 tig0020594:+1983 Msa1383310:intron
TCTGCAAATTTCTTTGGAGG+TGG 0.475452 tig0020594:-2907 None:intergenic
TTCAAATTCGAGCTTACAAA+GGG 0.481570 tig0020594:-2931 None:intergenic
TCGTCGTGTGAGGATGTCTA+TGG 0.489827 tig0020594:+2124 Msa1383310:CDS
GAGAAAGAGAAGGGTTCGTG+GGG 0.490414 tig0020594:-1101 None:intergenic
ACATCCACAACTCCAATGTC+AGG 0.493112 tig0020594:+3102 Msa1383310:CDS
GGTGTGACTTATTCATTCAA+TGG 0.499281 tig0020594:+3937 Msa1383310:three_prime_UTR
CTTCAGAAGCTGCTTTGTGG+TGG 0.503461 tig0020594:-1486 None:intergenic
CGCGAATTAGTGCTTGAGGT+TGG 0.505381 tig0020594:-2733 None:intergenic
TGAGTTTCAATTTATGAAGA+GGG 0.508341 tig0020594:+3263 Msa1383310:CDS
GCAAATTTCTTTGGAGGTGG+CGG 0.508534 tig0020594:-2904 None:intergenic
AGAGGATGAAATATAAACTT+AGG 0.508574 tig0020594:-4018 None:intergenic
TGTTTGAAGGTGAAGGAGGG+TGG 0.510225 tig0020594:+1391 Msa1383310:intron
AAGTAGAGAGAGAAAGAGAA+GGG 0.511516 tig0020594:-1110 None:intergenic
ATGGATGGCAGCCAAGCCTT+GGG 0.511965 tig0020594:+2831 Msa1383310:CDS
AGGTTATGGAGAAGTTCATC+AGG 0.515436 tig0020594:+1430 Msa1383310:CDS
GTCCTTGATGTTACTTGTGA+AGG 0.518061 tig0020594:+3541 Msa1383310:three_prime_UTR
TGACACCATGCTTGAAGTTG+AGG 0.519275 tig0020594:+4115 Msa1383310:three_prime_UTR
AAAATTCAAATGAGGCAAGA+AGG 0.523644 tig0020594:+2343 Msa1383310:CDS
TGCATACTCACTTGCTCAGA+AGG 0.524622 tig0020594:+2390 Msa1383310:CDS
ACCAAGGCAAAGCTAAAATC+GGG 0.526058 tig0020594:+3195 Msa1383310:CDS
TCACAAGTGTTCCCTGACAT+TGG 0.528516 tig0020594:-3114 None:intergenic
CCAAAACTCAGCAACACTCA+TGG 0.532255 tig0020594:+1229 Msa1383310:exon
GGTTATGAGTGCAGGCAAGA+AGG 0.532390 tig0020594:+1973 Msa1383310:intron
CGTTGTTAGAGCTCAACCTA+AGG 0.533918 tig0020594:+1558 Msa1383310:CDS
GTTATGAGTGCAGGCAAGAA+GGG 0.535330 tig0020594:+1974 Msa1383310:intron
ACTCTCATCGTACCGAAACT+CGG 0.535615 tig0020594:-3058 None:intergenic
TTCATTAAGCATATTCTGCA+CGG 0.537002 tig0020594:-2156 None:intergenic
TACCAAGAAGGCGCGGAAAG+TGG 0.545045 tig0020594:-1626 None:intergenic
ACTTCACCTACCAACCCAAT+CGG 0.545649 tig0020594:+3356 Msa1383310:CDS
CAATCTAGTCTCCCAAGGCT+TGG 0.546403 tig0020594:-2842 None:intergenic
CACCACAAAGCAGCTTCTGA+AGG 0.550489 tig0020594:+1487 Msa1383310:CDS
CAAATGGTATGAAAGCATAA+GGG 0.551950 tig0020594:+4277 Msa1383310:three_prime_UTR
GTGTAAGCTTCTGCAGCAGT+TGG 0.555988 tig0020594:-1520 None:intergenic
TTCGTCCAGATAATCTCTCC+AGG 0.556575 tig0020594:-3613 None:intergenic
GAAGTAGAGAGAGAAAGAGA+AGG 0.558166 tig0020594:-1111 None:intergenic
TTTGAGGGCATTGAAGGCAG+TGG 0.558218 tig0020594:+1998 Msa1383310:CDS
AGGAGATAACAGTTCATGGA+AGG 0.563394 tig0020594:+1450 Msa1383310:CDS
GAAAGAGAAGGGTTCGTGGG+GGG 0.563981 tig0020594:-1099 None:intergenic
ACTTATACTTTGAGAGTCAG+AGG 0.565119 tig0020594:-4036 None:intergenic
GCTCTAGGACCAATTCCAAA+TGG 0.565328 tig0020594:+4261 Msa1383310:three_prime_UTR
TCCTTGATGTTACTTGTGAA+GGG 0.565990 tig0020594:+3542 Msa1383310:three_prime_UTR
TTATGTTTGAAGGTGAAGGA+GGG 0.568504 tig0020594:+1388 Msa1383310:intron
ATGAGTTTCAATTTATGAAG+AGG 0.569955 tig0020594:+3262 Msa1383310:CDS
CGAGATGAACAAAGCTAATG+GGG 0.570107 tig0020594:+2789 Msa1383310:CDS
GAGAGTGGAAAATGAGGTGT+TGG 0.571769 tig0020594:-2758 None:intergenic
CAAAACTCAGCAACACTCAT+GGG 0.571912 tig0020594:+1230 Msa1383310:exon
AGAAAGAGAAGGGTTCGTGG+GGG 0.574053 tig0020594:-1100 None:intergenic
GAGCTCTTGTCCGATTGGGT+TGG 0.575108 tig0020594:-3366 None:intergenic
AGTATTGGTAATACTCAACC+TGG 0.575609 tig0020594:+3595 Msa1383310:three_prime_UTR
GAGTAAGAGCTCGTCGTGTG+AGG 0.575741 tig0020594:+2114 Msa1383310:CDS
TTTCAAATTCGAGCTTACAA+AGG 0.575937 tig0020594:-2932 None:intergenic
GTTCCCTGACATTGGAGTTG+TGG 0.575976 tig0020594:-3106 None:intergenic
CTCGAATTTGAAACCATGCT+CGG 0.576259 tig0020594:+2942 Msa1383310:CDS
GCTTGTCCAATATGGGGAGG+AGG 0.576470 tig0020594:-3009 None:intergenic
TCTTGTCCGATTGGGTTGGT+AGG 0.578767 tig0020594:-3362 None:intergenic
AGTGACATTAAGGTGCATGC+AGG 0.581722 tig0020594:+2070 Msa1383310:CDS
AGGATAGTTGTAACAGCACT+AGG 0.582943 tig0020594:+3145 Msa1383310:CDS
TCGTGTGAGGATGTCTATGG+AGG 0.584361 tig0020594:+2127 Msa1383310:CDS
TTCTGACCAATGCAAGCTCT+AGG 0.584781 tig0020594:+4246 Msa1383310:three_prime_UTR
GATTGGGTTGGTAGGTGAAG+TGG 0.587150 tig0020594:-3354 None:intergenic
CATCTCGTGATTCCGGAGAG+TGG 0.591885 tig0020594:-2773 None:intergenic
GTGTGATAGCATAGGAACAT+TGG 0.592155 tig0020594:+2306 Msa1383310:CDS
AATATGGGGAGGAGGCTTAG+CGG 0.592945 tig0020594:-3001 None:intergenic
TCCCTTCACAAGTAACATCA+AGG 0.592979 tig0020594:-3543 None:intergenic
AACCCAATCGGACAAGAGCT+CGG 0.593120 tig0020594:+3368 Msa1383310:CDS
GAGGATTCAAGCACTTGTCA+GGG 0.594722 tig0020594:+2022 Msa1383310:CDS
TTTCTTTGGAGGTGGCGGTG+GGG 0.595890 tig0020594:-2899 None:intergenic
ATTGGTCCTAGAGCTTGCAT+TGG 0.600573 tig0020594:-4252 Msa1383320:CDS
TTTATGTTTGAAGGTGAAGG+AGG 0.601205 tig0020594:+1387 Msa1383310:intron
ATGAACAATTATACAAATGG+AGG 0.601507 tig0020594:-3567 None:intergenic
ACCACAAAGCAGCTTCTGAA+GGG 0.605684 tig0020594:+1488 Msa1383310:CDS
AGGAGCTTTCAAAAGAGAGA+GGG 0.607085 tig0020594:+2363 Msa1383310:CDS
GGAGATAACAGTTCATGGAA+GGG 0.611321 tig0020594:+1451 Msa1383310:CDS
AGTGCTGTTACAACTATCCT+CGG 0.615531 tig0020594:-3142 None:intergenic
GAAGGGTGGTGTGATAGCAT+AGG 0.616172 tig0020594:+2298 Msa1383310:CDS
ACAAGAGCTCGGTGAGACCA+TGG 0.619435 tig0020594:+3379 Msa1383310:CDS
TCCATCAATCTAGTCTCCCA+AGG 0.620091 tig0020594:-2847 None:intergenic
AGAAGCTTACACTGCTGCTG+TGG 0.620813 tig0020594:+1531 Msa1383310:CDS
AAGGAGCTTTCAAAAGAGAG+AGG 0.623020 tig0020594:+2362 Msa1383310:CDS
GGGCATTGAAGGCAGTGGTG+AGG 0.626128 tig0020594:+2003 Msa1383310:CDS
ATAGTCCTCAACTTCAAGCA+TGG 0.629476 tig0020594:-4120 Msa1383320:CDS
ATTCCGGAGAGTGGAAAATG+AGG 0.631071 tig0020594:-2764 None:intergenic
CGAGTAGCTTGTCCAATATG+GGG 0.633366 tig0020594:-3015 None:intergenic
CGTGTGAGGATGTCTATGGA+GGG 0.633588 tig0020594:+2128 Msa1383310:CDS
GAGTTGGTTGGAAAGATGGA+TGG 0.634715 tig0020594:+2816 Msa1383310:CDS
TGTCAGGGAACACTTGTGAA+AGG 0.634990 tig0020594:+3118 Msa1383310:CDS
GGTTCGCGAATTAGTGCTTG+AGG 0.643363 tig0020594:-2737 None:intergenic
AAATGTATAACAGAGCTCAA+AGG 0.643845 tig0020594:+3226 Msa1383310:CDS
TTGTGAATCTGCAGGAAGGG+TGG 0.652606 tig0020594:+2284 Msa1383310:intron
AAAGGGAAATAGCGGCTGGG+TGG 0.656150 tig0020594:+3406 Msa1383310:three_prime_UTR
CATCCACAACTCCAATGTCA+GGG 0.657914 tig0020594:+3103 Msa1383310:CDS
CTTAGGTTGAGCTCTAACAA+CGG 0.664616 tig0020594:-1557 None:intergenic
GAAAGAGAGACACTTGTGCA+TGG 0.671235 tig0020594:+3961 Msa1383310:three_prime_UTR
AATTGAATACCAAGAAGGCG+CGG 0.680101 tig0020594:-1633 None:intergenic
AGGATTCAAGCACTTGTCAG+GGG 0.684889 tig0020594:+2023 Msa1383310:CDS
TTGTCAGGGGTAGACAAGTG+AGG 0.688660 tig0020594:+2036 Msa1383310:CDS
GTAGCTTGTCCAATATGGGG+AGG 0.689343 tig0020594:-3012 None:intergenic
GAACCTGACTCAATCAACCA+AGG 0.696026 tig0020594:+3179 Msa1383310:CDS
AAAAGAACAATGTCACAACA+AGG 0.709510 tig0020594:+2974 Msa1383310:CDS
GAGATGAACAAAGCTAATGG+GGG 0.721370 tig0020594:+2790 Msa1383310:CDS
GAACACTTGTGAAAGGACCG+AGG 0.744801 tig0020594:+3125 Msa1383310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTAAAAAAACATTAAAT+GGG - tig0020594:1789-1808 None:intergenic 10.0%
!! TAAACTAAAAAAACATTAAA+TGG - tig0020594:1790-1809 None:intergenic 10.0%
!! TTAAAAAATAACAAATTTGA+GGG - tig0020594:2473-2492 None:intergenic 10.0%
!! ATTAAAAAATAACAAATTTG+AGG - tig0020594:2474-2493 None:intergenic 10.0%
!!! TTATTTCTAATAGTAATTTA+TGG + tig0020594:3824-3843 Msa1383310:three_prime_UTR 10.0%
!!! AAAAAACAAACTTTTTTTGT+GGG - tig0020594:1342-1361 None:intergenic 15.0%
!!! AAAAAAACAAACTTTTTTTG+TGG - tig0020594:1343-1362 None:intergenic 15.0%
!!! AATGTTTTTTTAGTTTAGAT+TGG + tig0020594:1793-1812 Msa1383310:intron 15.0%
!! TTAAATCAAAAATTCAAATG+AGG + tig0020594:2335-2354 Msa1383310:CDS 15.0%
!! ACAAAATAAAATGAAAAACA+TGG - tig0020594:3672-3691 None:intergenic 15.0%
!!! AAGTATAAGTACAATTTTAA+CGG + tig0020594:4049-4068 Msa1383310:three_prime_UTR 15.0%
!! AAAATAAATAGTCATCAAAG+AGG - tig0020594:1710-1729 None:intergenic 20.0%
!!! TAGTTTAGATTGGAAAATTT+TGG + tig0020594:1803-1822 Msa1383310:intron 20.0%
!! ACTAAATTGAACAATTTGAA+AGG - tig0020594:2506-2525 None:intergenic 20.0%
!!! ATAATTTGAAATTTCGTTAG+AGG + tig0020594:2559-2578 Msa1383310:intron 20.0%
!! CTTGGAAAAACAAATAAATA+AGG - tig0020594:3445-3464 None:intergenic 20.0%
!! CAAATGAACAATTATACAAA+TGG - tig0020594:3573-3592 None:intergenic 20.0%
!! TGTTATACAACATGATTATT+TGG + tig0020594:3783-3802 Msa1383310:three_prime_UTR 20.0%
!! TATACAGAAAATACTTGTTT+CGG - tig0020594:4188-4207 Msa1383320:CDS 20.0%
!!! TTTATTGTGTTGACTATGTT+TGG + tig0020594:1364-1383 Msa1383310:intron 25.0%
!! TATGTTTGGTTTATGTTTGA+AGG + tig0020594:1378-1397 Msa1383310:intron 25.0%
! AAAGTAAAAAATGGAGGTTA+TGG + tig0020594:1416-1435 Msa1383310:CDS 25.0%
! GAAAAAATTGAATACCAAGA+AGG - tig0020594:1641-1660 None:intergenic 25.0%
!! TTTGGACATTCATCTATTTT+AGG + tig0020594:1821-1840 Msa1383310:intron 25.0%
!! TTGGACATTCATCTATTTTA+GGG + tig0020594:1822-1841 Msa1383310:intron 25.0%
! TAGTGTATTGAACTATTTGT+TGG + tig0020594:1922-1941 Msa1383310:intron 25.0%
!!! ATTTTATTTTGTGAATCTGC+AGG + tig0020594:2276-2295 Msa1383310:intron 25.0%
!! GTCTATGAATTTTCAAATCA+TGG + tig0020594:2536-2555 Msa1383310:intron 25.0%
! TATTAGTTCCTAAAGTTGTA+GGG - tig0020594:2600-2619 None:intergenic 25.0%
! TTATTAGTTCCTAAAGTTGT+AGG - tig0020594:2601-2620 None:intergenic 25.0%
! GTTTATTCATTCAAACATAG+TGG + tig0020594:2624-2643 Msa1383310:intron 25.0%
! GAAAGGAAAGAAAAAGAAAA+AGG - tig0020594:2669-2688 None:intergenic 25.0%
! ATGAGTTTCAATTTATGAAG+AGG + tig0020594:3262-3281 Msa1383310:CDS 25.0%
! TGAGTTTCAATTTATGAAGA+GGG + tig0020594:3263-3282 Msa1383310:CDS 25.0%
! ATGAACAATTATACAAATGG+AGG - tig0020594:3570-3589 None:intergenic 25.0%
! AATTGTTCATTTGTGAGTAT+TGG + tig0020594:3580-3599 Msa1383310:three_prime_UTR 25.0%
!!! TTTTATTTTGTGAGTGTTCA+TGG + tig0020594:3681-3700 Msa1383310:three_prime_UTR 25.0%
! AGAGGATGAAATATAAACTT+AGG - tig0020594:4021-4040 None:intergenic 25.0%
! AAATGGGTCAAAGCATTAAT+CGG + tig0020594:1263-1282 Msa1383310:CDS 30.0%
AGGGTGGTAAAAGTAAAAAA+TGG + tig0020594:1407-1426 Msa1383310:CDS 30.0%
GTGGTAAAAGTAAAAAATGG+AGG + tig0020594:1410-1429 Msa1383310:CDS 30.0%
! CTAACAAGTCTAAAATCCTT+AGG - tig0020594:1577-1596 None:intergenic 30.0%
!! CTAAGGATTTTAGACTTGTT+AGG + tig0020594:1575-1594 Msa1383310:CDS 30.0%
!! AAAACAAAAGCTTGAGTTGA+AGG - tig0020594:1666-1685 None:intergenic 30.0%
! GTATTGAACTATTTGTTGGA+AGG + tig0020594:1926-1945 Msa1383310:intron 30.0%
TGTGTTTGTAATGAATGTGA+GGG + tig0020594:1952-1971 Msa1383310:intron 30.0%
TTCATTAAGCATATTCTGCA+CGG - tig0020594:2159-2178 None:intergenic 30.0%
AAAATTCAAATGAGGCAAGA+AGG + tig0020594:2343-2362 Msa1383310:CDS 30.0%
AGGAAAGAAAAAGAAAAAGG+TGG - tig0020594:2666-2685 None:intergenic 30.0%
AAGCATCTTTCTTTGTAGAA+AGG - tig0020594:2686-2705 None:intergenic 30.0%
TTCAAATTCGAGCTTACAAA+GGG - tig0020594:2934-2953 None:intergenic 30.0%
TTTCAAATTCGAGCTTACAA+AGG - tig0020594:2935-2954 None:intergenic 30.0%
AAAAGAACAATGTCACAACA+AGG + tig0020594:2974-2993 Msa1383310:CDS 30.0%
AAATGTATAACAGAGCTCAA+AGG + tig0020594:3226-3245 Msa1383310:CDS 30.0%
! ATCATTCACTCAACTTTTCT+AGG + tig0020594:3331-3350 Msa1383310:CDS 30.0%
TGGACGAATTGTCAATTTAT+GGG + tig0020594:3628-3647 Msa1383310:three_prime_UTR 30.0%
TTGATTCAATGGCAAAAACT+AGG - tig0020594:3892-3911 None:intergenic 30.0%
!! GGTGAGATTTATTGATTCAA+TGG - tig0020594:3903-3922 None:intergenic 30.0%
TCTCACCATTAGATGATATA+TGG + tig0020594:3916-3935 Msa1383310:three_prime_UTR 30.0%
TCACACCATATATCATCTAA+TGG - tig0020594:3924-3943 None:intergenic 30.0%
! ATTCCATATTCGAGTTCTAA+AGG + tig0020594:4141-4160 Msa1383310:three_prime_UTR 30.0%
!! TTCCATATTCGAGTTCTAAA+GGG + tig0020594:4142-4161 Msa1383310:three_prime_UTR 30.0%
! CAAATGGTATGAAAGCATAA+GGG + tig0020594:4277-4296 Msa1383310:three_prime_UTR 30.0%
AAGTAGAGAGAGAAAGAGAA+GGG - tig0020594:1113-1132 None:intergenic 35.0%
ACATGTTGTTCATCTTTGTC+TGG - tig0020594:1299-1318 None:intergenic 35.0%
TGGTTTATGTTTGAAGGTGA+AGG + tig0020594:1384-1403 Msa1383310:intron 35.0%
TTTATGTTTGAAGGTGAAGG+AGG + tig0020594:1387-1406 Msa1383310:intron 35.0%
TTATGTTTGAAGGTGAAGGA+GGG + tig0020594:1388-1407 Msa1383310:intron 35.0%
!! TTAGACTTGTTAGGCAAGAA+TGG + tig0020594:1584-1603 Msa1383310:CDS 35.0%
!! TAGACTTGTTAGGCAAGAAT+GGG + tig0020594:1585-1604 Msa1383310:CDS 35.0%
CTGTGTTTGTAATGAATGTG+AGG + tig0020594:1951-1970 Msa1383310:intron 35.0%
! CAGCAAACTTGAACTTTTGA+AGG + tig0020594:2184-2203 Msa1383310:CDS 35.0%
TATGTGTTGTGAATTGACAG+TGG + tig0020594:2216-2235 Msa1383310:intron 35.0%
! TATTTTGTGAATCTGCAGGA+AGG + tig0020594:2280-2299 Msa1383310:intron 35.0%
! ATTTTGTGAATCTGCAGGAA+GGG + tig0020594:2281-2300 Msa1383310:intron 35.0%
! TTAAGTTGCCCTACAACTTT+AGG + tig0020594:2589-2608 Msa1383310:intron 35.0%
ACGAGATGAACAAAGCTAAT+GGG + tig0020594:2788-2807 Msa1383310:CDS 35.0%
AAGGGTTCTGCAAATTTCTT+TGG - tig0020594:2916-2935 None:intergenic 35.0%
TGAGTCAGGTTCATGTAATT+TGG - tig0020594:3171-3190 None:intergenic 35.0%
AACCAAGGCAAAGCTAAAAT+CGG + tig0020594:3194-3213 Msa1383310:CDS 35.0%
!!! TATGCTTTTAGAATCTCCGT+TGG - tig0020594:3301-3320 None:intergenic 35.0%
ATGGTGAAAAAGGGAAATAG+CGG + tig0020594:3398-3417 Msa1383310:exon 35.0%
CAACAAGATGTTGAACTTCT+TGG - tig0020594:3463-3482 None:intergenic 35.0%
! TCCTTGATGTTACTTGTGAA+GGG + tig0020594:3542-3561 Msa1383310:three_prime_UTR 35.0%
!! AGTATTGGTAATACTCAACC+TGG + tig0020594:3595-3614 Msa1383310:three_prime_UTR 35.0%
CTGGACGAATTGTCAATTTA+TGG + tig0020594:3627-3646 Msa1383310:three_prime_UTR 35.0%
TGTCTTGTGCATCATAGAAT+TGG - tig0020594:3850-3869 None:intergenic 35.0%
GGTGTGACTTATTCATTCAA+TGG + tig0020594:3937-3956 Msa1383310:three_prime_UTR 35.0%
! ACTTATACTTTGAGAGTCAG+AGG - tig0020594:4039-4058 None:intergenic 35.0%
TGAAAGCTTGATCTCTTGTA+TGG + tig0020594:4218-4237 Msa1383310:three_prime_UTR 35.0%
! GCTTTCATACCATTTGGAAT+TGG - tig0020594:4273-4292 Msa1383320:CDS 35.0%
CCTTATGCTTTCATACCATT+TGG - tig0020594:4279-4298 Msa1383320:CDS 35.0%
! CCAAATGGTATGAAAGCATA+AGG + tig0020594:4276-4295 Msa1383310:three_prime_UTR 35.0%
GAAGTAGAGAGAGAAAGAGA+AGG - tig0020594:1114-1133 None:intergenic 40.0%
CAAAACTCAGCAACACTCAT+GGG + tig0020594:1230-1249 Msa1383310:exon 40.0%
AGGTTATGGAGAAGTTCATC+AGG + tig0020594:1430-1449 Msa1383310:CDS 40.0%
CATCAGGAGATAACAGTTCA+TGG + tig0020594:1446-1465 Msa1383310:CDS 40.0%
AGGAGATAACAGTTCATGGA+AGG + tig0020594:1450-1469 Msa1383310:CDS 40.0%
GGAGATAACAGTTCATGGAA+GGG + tig0020594:1451-1470 Msa1383310:CDS 40.0%
!! CTTAGGTTGAGCTCTAACAA+CGG - tig0020594:1560-1579 None:intergenic 40.0%
AATTGAATACCAAGAAGGCG+CGG - tig0020594:1636-1655 None:intergenic 40.0%
!! TGAACTTTTGAAGGAAGCAG+AGG + tig0020594:2193-2212 Msa1383310:CDS 40.0%
GTGTGATAGCATAGGAACAT+TGG + tig0020594:2306-2325 Msa1383310:CDS 40.0%
AAGGAGCTTTCAAAAGAGAG+AGG + tig0020594:2362-2381 Msa1383310:CDS 40.0%
AGGAGCTTTCAAAAGAGAGA+GGG + tig0020594:2363-2382 Msa1383310:CDS 40.0%
! TCTCTTTTCAGTCCTGAGTA+AGG + tig0020594:2433-2452 Msa1383310:intron 40.0%
! TTTGAGATTTCCAGCAATGC+AGG + tig0020594:2710-2729 Msa1383310:intron 40.0%
CTTTGTTCATCTCGTGATTC+CGG - tig0020594:2783-2802 None:intergenic 40.0%
CACGAGATGAACAAAGCTAA+TGG + tig0020594:2787-2806 Msa1383310:CDS 40.0%
CGAGATGAACAAAGCTAATG+GGG + tig0020594:2789-2808 Msa1383310:CDS 40.0%
GAGATGAACAAAGCTAATGG+GGG + tig0020594:2790-2809 Msa1383310:CDS 40.0%
!!! TGGGGTTTTTTCTAAGCTCT+CGG - tig0020594:2884-2903 None:intergenic 40.0%
! TCTGCAAATTTCTTTGGAGG+TGG - tig0020594:2910-2929 None:intergenic 40.0%
GGTTCTGCAAATTTCTTTGG+AGG - tig0020594:2913-2932 None:intergenic 40.0%
! CTCGAATTTGAAACCATGCT+CGG + tig0020594:2942-2961 Msa1383310:CDS 40.0%
!!! TTTTTGACTTTGACCGAGCA+TGG - tig0020594:2958-2977 None:intergenic 40.0%
! ACGAGTAGCTTGTCCAATAT+GGG - tig0020594:3019-3038 None:intergenic 40.0%
! AACGAGTAGCTTGTCCAATA+TGG - tig0020594:3020-3039 None:intergenic 40.0%
! AGTGCTGTTACAACTATCCT+CGG - tig0020594:3145-3164 None:intergenic 40.0%
AGGATAGTTGTAACAGCACT+AGG + tig0020594:3145-3164 Msa1383310:CDS 40.0%
ACCAAGGCAAAGCTAAAATC+GGG + tig0020594:3195-3214 Msa1383310:CDS 40.0%
!! GCTATTTCCCTTTTTCACCA+TGG - tig0020594:3399-3418 None:intergenic 40.0%
! GTCCTTGATGTTACTTGTGA+AGG + tig0020594:3541-3560 Msa1383310:three_prime_UTR 40.0%
TCCCTTCACAAGTAACATCA+AGG - tig0020594:3546-3565 None:intergenic 40.0%
!!! GTGAGTGTTCATGGTTTTGT+AGG + tig0020594:3690-3709 Msa1383310:three_prime_UTR 40.0%
ATAGTCCTCAACTTCAAGCA+TGG - tig0020594:4123-4142 Msa1383320:CDS 40.0%
!! AGCCCTTTAGAACTCGAATA+TGG - tig0020594:4147-4166 Msa1383320:CDS 40.0%
TCTAAAGGGCTTTCCATATC+AGG + tig0020594:4156-4175 Msa1383310:three_prime_UTR 40.0%
AGAGAAAGAGAAGGGTTCGT+GGG - tig0020594:1105-1124 None:intergenic 45.0%
AGAGAGAGAGAGAGTGAATG+TGG - tig0020594:1194-1213 None:intergenic 45.0%
!! CCATGAGTGTTGCTGAGTTT+TGG - tig0020594:1232-1251 None:intergenic 45.0%
CCAAAACTCAGCAACACTCA+TGG + tig0020594:1229-1248 Msa1383310:exon 45.0%
! TCATGGGTGCTTCTGGAAAA+TGG + tig0020594:1246-1265 Msa1383310:exon 45.0%
! CATGGGTGCTTCTGGAAAAT+GGG + tig0020594:1247-1266 Msa1383310:exon 45.0%
ACCACAAAGCAGCTTCTGAA+GGG + tig0020594:1488-1507 Msa1383310:CDS 45.0%
CGTTGTTAGAGCTCAACCTA+AGG + tig0020594:1558-1577 Msa1383310:CDS 45.0%
AATGTGAGGGTTATGAGTGC+AGG + tig0020594:1965-1984 Msa1383310:intron 45.0%
GTTATGAGTGCAGGCAAGAA+GGG + tig0020594:1974-1993 Msa1383310:intron 45.0%
TGAGGATTCAAGCACTTGTC+AGG + tig0020594:2021-2040 Msa1383310:CDS 45.0%
GAGGATTCAAGCACTTGTCA+GGG + tig0020594:2022-2041 Msa1383310:CDS 45.0%
AGGATTCAAGCACTTGTCAG+GGG + tig0020594:2023-2042 Msa1383310:CDS 45.0%
AGCAAGCTGCAGTGACATTA+AGG + tig0020594:2060-2079 Msa1383310:CDS 45.0%
AGTGACATTAAGGTGCATGC+AGG + tig0020594:2070-2089 Msa1383310:CDS 45.0%
TGCATACTCACTTGCTCAGA+AGG + tig0020594:2390-2409 Msa1383310:CDS 45.0%
TAATTGACGACGCCTTACTC+AGG - tig0020594:2448-2467 None:intergenic 45.0%
GAGAGTGGAAAATGAGGTGT+TGG - tig0020594:2761-2780 None:intergenic 45.0%
! ACACCTCATTTTCCACTCTC+CGG + tig0020594:2761-2780 Msa1383310:CDS 45.0%
ATTCCGGAGAGTGGAAAATG+AGG - tig0020594:2767-2786 None:intergenic 45.0%
TGAACAAAGCTAATGGGGGA+TGG + tig0020594:2794-2813 Msa1383310:CDS 45.0%
! GATGGAGTTGGTTGGAAAGA+TGG + tig0020594:2812-2831 Msa1383310:CDS 45.0%
! GAGTTGGTTGGAAAGATGGA+TGG + tig0020594:2816-2835 Msa1383310:CDS 45.0%
TCCATCAATCTAGTCTCCCA+AGG - tig0020594:2850-2869 None:intergenic 45.0%
! GCAAATTTCTTTGGAGGTGG+CGG - tig0020594:2907-2926 None:intergenic 45.0%
! CGAGTAGCTTGTCCAATATG+GGG - tig0020594:3018-3037 None:intergenic 45.0%
CAAGTCCAAGTTCCGAGTTT+CGG + tig0020594:3046-3065 Msa1383310:CDS 45.0%
ACTCTCATCGTACCGAAACT+CGG - tig0020594:3061-3080 None:intergenic 45.0%
ACATCCACAACTCCAATGTC+AGG + tig0020594:3102-3121 Msa1383310:CDS 45.0%
CATCCACAACTCCAATGTCA+GGG + tig0020594:3103-3122 Msa1383310:CDS 45.0%
TCACAAGTGTTCCCTGACAT+TGG - tig0020594:3117-3136 None:intergenic 45.0%
TGTCAGGGAACACTTGTGAA+AGG + tig0020594:3118-3137 Msa1383310:CDS 45.0%
GAACCTGACTCAATCAACCA+AGG + tig0020594:3179-3198 Msa1383310:CDS 45.0%
TTGCCTTGGTTGATTGAGTC+AGG - tig0020594:3185-3204 None:intergenic 45.0%
!!! ACCCGATTTTAGCTTTGCCT+TGG - tig0020594:3199-3218 None:intergenic 45.0%
AGCTCAAAGGCAACAGTCAA+TGG + tig0020594:3239-3258 Msa1383310:CDS 45.0%
ACTTCACCTACCAACCCAAT+CGG + tig0020594:3356-3375 Msa1383310:CDS 45.0%
GGTGAGACCATGGTGAAAAA+GGG + tig0020594:3389-3408 Msa1383310:exon 45.0%
TGAAAAAGGGAAATAGCGGC+TGG + tig0020594:3402-3421 Msa1383310:exon 45.0%
GAAAAAGGGAAATAGCGGCT+GGG + tig0020594:3403-3422 Msa1383310:exon 45.0%
CTCAACCTGGAGAGATTATC+TGG + tig0020594:3608-3627 Msa1383310:three_prime_UTR 45.0%
TTCGTCCAGATAATCTCTCC+AGG - tig0020594:3616-3635 None:intergenic 45.0%
GAAAGAGAGACACTTGTGCA+TGG + tig0020594:3961-3980 Msa1383310:three_prime_UTR 45.0%
TGACACCATGCTTGAAGTTG+AGG + tig0020594:4115-4134 Msa1383310:three_prime_UTR 45.0%
GTTTCGGCATTCACCTGATA+TGG - tig0020594:4172-4191 Msa1383320:CDS 45.0%
TTCTGACCAATGCAAGCTCT+AGG + tig0020594:4246-4265 Msa1383310:three_prime_UTR 45.0%
ATTGGTCCTAGAGCTTGCAT+TGG - tig0020594:4255-4274 Msa1383320:CDS 45.0%
!! GCTCTAGGACCAATTCCAAA+TGG + tig0020594:4261-4280 Msa1383310:three_prime_UTR 45.0%
AGAAAGAGAAGGGTTCGTGG+GGG - tig0020594:1103-1122 None:intergenic 50.0%
GAGAAAGAGAAGGGTTCGTG+GGG - tig0020594:1104-1123 None:intergenic 50.0%
GAGAGAAAGAGAAGGGTTCG+TGG - tig0020594:1106-1125 None:intergenic 50.0%
TGTTTGAAGGTGAAGGAGGG+TGG + tig0020594:1391-1410 Msa1383310:intron 50.0%
! CTTCAGAAGCTGCTTTGTGG+TGG - tig0020594:1489-1508 None:intergenic 50.0%
CACCACAAAGCAGCTTCTGA+AGG + tig0020594:1487-1506 Msa1383310:CDS 50.0%
! ACCCTTCAGAAGCTGCTTTG+TGG - tig0020594:1492-1511 None:intergenic 50.0%
GTGTAAGCTTCTGCAGCAGT+TGG - tig0020594:1523-1542 None:intergenic 50.0%
AGAAGCTTACACTGCTGCTG+TGG + tig0020594:1531-1550 Msa1383310:CDS 50.0%
GGTTATGAGTGCAGGCAAGA+AGG + tig0020594:1973-1992 Msa1383310:intron 50.0%
!! AAGGGCTTTGAGGGCATTGA+AGG + tig0020594:1992-2011 Msa1383310:CDS 50.0%
!! TTTGAGGGCATTGAAGGCAG+TGG + tig0020594:1998-2017 Msa1383310:CDS 50.0%
TTGTCAGGGGTAGACAAGTG+AGG + tig0020594:2036-2055 Msa1383310:CDS 50.0%
TCGTCGTGTGAGGATGTCTA+TGG + tig0020594:2124-2143 Msa1383310:CDS 50.0%
TCGTGTGAGGATGTCTATGG+AGG + tig0020594:2127-2146 Msa1383310:CDS 50.0%
CGTGTGAGGATGTCTATGGA+GGG + tig0020594:2128-2147 Msa1383310:CDS 50.0%
TTGTGAATCTGCAGGAAGGG+TGG + tig0020594:2284-2303 Msa1383310:intron 50.0%
GAAGGGTGGTGTGATAGCAT+AGG + tig0020594:2298-2317 Msa1383310:CDS 50.0%
CGCGAATTAGTGCTTGAGGT+TGG - tig0020594:2736-2755 None:intergenic 50.0%
GGTTCGCGAATTAGTGCTTG+AGG - tig0020594:2740-2759 None:intergenic 50.0%
AAGCTAATGGGGGATGGAGT+TGG + tig0020594:2800-2819 Msa1383310:CDS 50.0%
! TAATGGGGGATGGAGTTGGT+TGG + tig0020594:2804-2823 Msa1383310:CDS 50.0%
CAATCTAGTCTCCCAAGGCT+TGG - tig0020594:2845-2864 None:intergenic 50.0%
GCCTTGGGAGACTAGATTGA+TGG + tig0020594:2846-2865 Msa1383310:CDS 50.0%
! ATTTCTTTGGAGGTGGCGGT+GGG - tig0020594:2903-2922 None:intergenic 50.0%
! AATTTCTTTGGAGGTGGCGG+TGG - tig0020594:2904-2923 None:intergenic 50.0%
AATATGGGGAGGAGGCTTAG+CGG - tig0020594:3004-3023 None:intergenic 50.0%
! GTAGCTTGTCCAATATGGGG+AGG - tig0020594:3015-3034 None:intergenic 50.0%
TCGTACCGAAACTCGGAACT+TGG - tig0020594:3054-3073 None:intergenic 50.0%
GTTCCCTGACATTGGAGTTG+TGG - tig0020594:3109-3128 None:intergenic 50.0%
GAACACTTGTGAAAGGACCG+AGG + tig0020594:3125-3144 Msa1383310:CDS 50.0%
! AGGGCTGCAGTTTTCTCCAA+CGG + tig0020594:3282-3301 Msa1383310:CDS 50.0%
!! GATTGGGTTGGTAGGTGAAG+TGG - tig0020594:3357-3376 None:intergenic 50.0%
!! TCTTGTCCGATTGGGTTGGT+AGG - tig0020594:3365-3384 None:intergenic 50.0%
AACCCAATCGGACAAGAGCT+CGG + tig0020594:3368-3387 Msa1383310:CDS 50.0%
CGGTGAGACCATGGTGAAAA+AGG + tig0020594:3388-3407 Msa1383310:exon 50.0%
GAAAGAGAAGGGTTCGTGGG+GGG - tig0020594:1102-1121 None:intergenic 55.0%
GCAACACTCATGGGTGCTTC+TGG + tig0020594:1239-1258 Msa1383310:exon 55.0%
! AGAAGGCGCGGAAAGTGGTT+TGG - tig0020594:1624-1643 None:intergenic 55.0%
AACCACTTTCCGCGCCTTCT+TGG + tig0020594:1624-1643 Msa1383310:CDS 55.0%
! TACCAAGAAGGCGCGGAAAG+TGG - tig0020594:1629-1648 None:intergenic 55.0%
TGCAGGCAAGAAGGGCTTTG+AGG + tig0020594:1982-2001 Msa1383310:intron 55.0%
! GCAGGCAAGAAGGGCTTTGA+GGG + tig0020594:1983-2002 Msa1383310:intron 55.0%
GAGTAAGAGCTCGTCGTGTG+AGG + tig0020594:2114-2133 Msa1383310:CDS 55.0%
!! TTGAGGTTGGCCTGCATTGC+TGG - tig0020594:2723-2742 None:intergenic 55.0%
CATCTCGTGATTCCGGAGAG+TGG - tig0020594:2776-2795 None:intergenic 55.0%
ATGGATGGCAGCCAAGCCTT+GGG + tig0020594:2831-2850 Msa1383310:CDS 55.0%
! TTTCTTTGGAGGTGGCGGTG+GGG - tig0020594:2902-2921 None:intergenic 55.0%
GCTAAGCCTCCTCCCCATAT+TGG + tig0020594:3003-3022 Msa1383310:CDS 55.0%
GCTTGTCCAATATGGGGAGG+AGG - tig0020594:3012-3031 None:intergenic 55.0%
GAGCTCTTGTCCGATTGGGT+TGG - tig0020594:3369-3388 None:intergenic 55.0%
CACCGAGCTCTTGTCCGATT+GGG - tig0020594:3373-3392 None:intergenic 55.0%
TCACCGAGCTCTTGTCCGAT+TGG - tig0020594:3374-3393 None:intergenic 55.0%
! ACAAGAGCTCGGTGAGACCA+TGG + tig0020594:3379-3398 Msa1383310:CDS 55.0%
AAAGGGAAATAGCGGCTGGG+TGG + tig0020594:3406-3425 Msa1383310:three_prime_UTR 55.0%
!! GGGCATTGAAGGCAGTGGTG+AGG + tig0020594:2003-2022 Msa1383310:CDS 60.0%
GATGGATGGCAGCCAAGCCT+TGG + tig0020594:2830-2849 Msa1383310:CDS 60.0%
AGAAGGGTTCGTGGGGGGTG+TGG - tig0020594:1097-1116 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0020594 gene 1088 4307 1088 ID=Msa1383310;Name=Msa1383310
tig0020594 mRNA 1088 4307 1088 ID=Msa1383310-mRNA-1;Parent=Msa1383310;Name=Msa1383310-mRNA-1;_AED=0.00;_eAED=0.00;_QI=160|1|1|1|1|1|5|903|445
tig0020594 exon 1088 1316 1088 ID=Msa1383310-mRNA-1:exon:8522;Parent=Msa1383310-mRNA-1
tig0020594 exon 1400 1645 1400 ID=Msa1383310-mRNA-1:exon:8523;Parent=Msa1383310-mRNA-1
tig0020594 exon 1987 2214 1987 ID=Msa1383310-mRNA-1:exon:8524;Parent=Msa1383310-mRNA-1
tig0020594 exon 2298 2411 2298 ID=Msa1383310-mRNA-1:exon:8525;Parent=Msa1383310-mRNA-1
tig0020594 exon 2724 4307 2724 ID=Msa1383310-mRNA-1:exon:8526;Parent=Msa1383310-mRNA-1
tig0020594 five_prime_UTR 1088 1247 1088 ID=Msa1383310-mRNA-1:five_prime_utr;Parent=Msa1383310-mRNA-1
tig0020594 CDS 1248 1316 1248 ID=Msa1383310-mRNA-1:cds;Parent=Msa1383310-mRNA-1
tig0020594 CDS 1400 1645 1400 ID=Msa1383310-mRNA-1:cds;Parent=Msa1383310-mRNA-1
tig0020594 CDS 1987 2214 1987 ID=Msa1383310-mRNA-1:cds;Parent=Msa1383310-mRNA-1
tig0020594 CDS 2298 2411 2298 ID=Msa1383310-mRNA-1:cds;Parent=Msa1383310-mRNA-1
tig0020594 CDS 2724 3404 2724 ID=Msa1383310-mRNA-1:cds;Parent=Msa1383310-mRNA-1
tig0020594 three_prime_UTR 3405 4307 3405 ID=Msa1383310-mRNA-1:three_prime_utr;Parent=Msa1383310-mRNA-1
Gene Sequence

>Msa1383310

ATGGGTGCTTCTGGAAAATGGGTCAAAGCATTAATCGGATTCAAGAAACCAGACAAAGATGAACAACATGTGAAGGAGGGTGGTAAAAGTAAAAAATGGAGGTTATGGAGAAGTTCATCAGGAGATAACAGTTCATGGAAGGGTTTCAAAACAAACCACCACAAAGCAGCTTCTGAAGGGTCTGAATCTCCAACTGCTGCAGAAGCTTACACTGCTGCTGTGGCTACCGTTGTTAGAGCTCAACCTAAGGATTTTAGACTTGTTAGGCAAGAATGGGCTGCTATAAGAATCCAAACCACTTTCCGCGCCTTCTTGGCAAGAAGGGCTTTGAGGGCATTGAAGGCAGTGGTGAGGATTCAAGCACTTGTCAGGGGTAGACAAGTGAGGAAGCAAGCTGCAGTGACATTAAGGTGCATGCAGGCATTAGTTCGTGTTCAAGCTCGAGTAAGAGCTCGTCGTGTGAGGATGTCTATGGAGGGACAAGCCGTGCAGAATATGCTTAATGAACGACGCAGCAAACTTGAACTTTTGAAGGAAGCAGAGGAAGGGTGGTGTGATAGCATAGGAACATTGGAAGATGTTAAATCAAAAATTCAAATGAGGCAAGAAGGAGCTTTCAAAAGAGAGAGGGCACTTGCATACTCACTTGCTCAGAAGCAATGCAGGCCAACCTCAAGCACTAATTCGCGAACCAACACCTCATTTTCCACTCTCCGGAATCACGAGATGAACAAAGCTAATGGGGGATGGAGTTGGTTGGAAAGATGGATGGCAGCCAAGCCTTGGGAGACTAGATTGATGGAACAGTCTCACGCCGAGAGCTTAGAAAAAACCCCACCGCCACCTCCAAAGAAATTTGCAGAACCCTTTGTAAGCTCGAATTTGAAACCATGCTCGGTCAAAGTCAAAAAGAACAATGTCACAACAAGGATTTCCGCTAAGCCTCCTCCCCATATTGGACAAGCTACTCGTTCATCGTCAAGTCCAAGTTCCGAGTTTCGGTACGATGAGAGTTCTGCATCATCTTCTATTTGTACATCCACAACTCCAATGTCAGGGAACACTTGTGAAAGGACCGAGGATAGTTGTAACAGCACTAGGCCAAATTACATGAACCTGACTCAATCAACCAAGGCAAAGCTAAAATCGGGTAGTAATCAAATGTATAACAGAGCTCAAAGGCAACAGTCAATGGATGAGTTTCAATTTATGAAGAGGGCTGCAGTTTTCTCCAACGGAGATTCTAAAAGCATAGCTGCTTCTGATCATTCACTCAACTTTTCTAGGCCACTTCACCTACCAACCCAATCGGACAAGAGCTCGGTGAGACCATGGTGA
Protein sequence

>Msa1383310

MGASGKWVKALIGFKKPDKDEQHVKEGGKSKKWRLWRSSSGDNSSWKGFKTNHHKAASEGSESPTAAEAYTAAVATVVRAQPKDFRLVRQEWAAIRIQTTFRAFLARRALRALKAVVRIQALVRGRQVRKQAAVTLRCMQALVRVQARVRARRVRMSMEGQAVQNMLNERRSKLELLKEAEEGWCDSIGTLEDVKSKIQMRQEGAFKRERALAYSLAQKQCRPTSSTNSRTNTSFSTLRNHEMNKANGGWSWLERWMAAKPWETRLMEQSHAESLEKTPPPPPKKFAEPFVSSNLKPCSVKVKKNNVTTRISAKPPPHIGQATRSSSSPSSEFRYDESSASSSICTSTTPMSGNTCERTEDSCNSTRPNYMNLTQSTKAKLKSGSNQMYNRAQRQQSMDEFQFMKRAAVFSNGDSKSIAASDHSLNFSRPLHLPTQSDKSSVRPW*