AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0605350


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605350 MtrunA17_Chr4g0034581 91.346 104 9 0 1 104 1 104 1.86e-68 200
Msa0605350 MtrunA17_Chr4g0034531 73.077 104 28 0 1 104 1 104 1.13e-52 160
Msa0605350 MtrunA17_Chr4g0034561 55.238 105 45 2 1 104 1 104 2.01e-37 122
Msa0605350 MtrunA17_Chr4g0034571 42.857 105 52 2 1 105 1 97 2.66e-21 84.0
Msa0605350 MtrunA17_Chr7g0234401 41.748 103 57 1 2 104 5 104 8.68e-21 79.7
Msa0605350 MtrunA17_Chr5g0445131 33.654 104 60 4 2 104 7 102 1.83e-11 55.8
Msa0605350 MtrunA17_Chr3g0100281 36.667 90 49 3 7 96 14 95 4.49e-11 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605350 AT5G48490.1 36.634 101 60 2 5 104 4 101 4.02e-14 63.2
Msa0605350 AT5G48485.1 41.558 77 42 1 28 104 29 102 6.02e-14 62.8

Find 32 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGGGTTGCTTTCTTTCTA+TGG 0.215569 4_2:+51165867 Msa0605350:CDS
CTATGTCGTTATAAGGATTC+AGG 0.249484 4_2:+51165849 Msa0605350:CDS
TCCTGTTAACTGTAAGCTTA+TGG 0.254280 4_2:+51165917 Msa0605350:CDS
TCAATGCCTATGTCGTTATA+AGG 0.293667 4_2:+51165842 Msa0605350:CDS
AATACCATGATGTTGGTTAA+TGG 0.374528 4_2:+51165693 Msa0605350:CDS
AGTGGTGACAATTCTGTTAA+TGG 0.378651 4_2:+51165771 Msa0605350:CDS
TCCATAAGCTTACAGTTAAC+AGG 0.382556 4_2:-51165918 None:intergenic
GGAAGTTCCATGGCTTGGTT+AGG 0.427875 4_2:-51165897 None:intergenic
TAGGCATTGAAGATCGGCTT+TGG 0.464541 4_2:-51165829 None:intergenic
ATTAACCAACATCATGGTAT+TGG 0.470830 4_2:-51165691 None:intergenic
TAACAGGAAGTTCCATGGCT+TGG 0.486774 4_2:-51165902 None:intergenic
TATGATTGTGGCGATGTTGT+TGG 0.507427 4_2:+51165662 Msa0605350:CDS
TATGTCGTTATAAGGATTCA+GGG 0.530226 4_2:+51165850 Msa0605350:CDS
CTGAATCCTTATAACGACAT+AGG 0.550946 4_2:-51165848 None:intergenic
AGAGATGGAGGCATACAAGC+AGG 0.551353 4_2:+51165638 None:intergenic
AACAAGGACTGAAGGCATGC+GGG 0.556452 4_2:+51165739 Msa0605350:CDS
GCCGTCTGATGCTTGCTGCT+CGG 0.561297 4_2:+51165800 Msa0605350:CDS
ACAGTTAACAGGAAGTTCCA+TGG 0.568806 4_2:-51165907 None:intergenic
CTGACCATTAACCAACATCA+TGG 0.570185 4_2:-51165697 None:intergenic
GCCGAGCAGCAAGCATCAGA+CGG 0.582886 4_2:-51165801 None:intergenic
ACGACATAGGCATTGAAGAT+CGG 0.583458 4_2:-51165835 None:intergenic
GAATTGTCACCACTCACATA+CGG 0.587779 4_2:-51165762 None:intergenic
CATGACAAAACAAGGACTGA+AGG 0.588836 4_2:+51165731 Msa0605350:CDS
ATACAAGCAGGTTATGATTG+TGG 0.602790 4_2:+51165650 Msa0605350:CDS
CAACATCATGGTATTGGCAA+TGG 0.605291 4_2:-51165685 None:intergenic
CATTGCCAATACCATGATGT+TGG 0.609484 4_2:+51165686 Msa0605350:CDS
TTATGTCACATGACAAAACA+AGG 0.627195 4_2:+51165723 Msa0605350:CDS
AAACAAGGACTGAAGGCATG+CGG 0.631589 4_2:+51165738 Msa0605350:CDS
TATTGATCCTAACCAAGCCA+TGG 0.632800 4_2:+51165890 Msa0605350:CDS
AACCACATGACTAGTAGCAG+TGG 0.639755 4_2:-51165947 None:intergenic
GCATGCGGGCCGTATGTGAG+TGG 0.668512 4_2:+51165753 Msa0605350:CDS
ACATGACTAGTAGCAGTGGA+AGG 0.673463 4_2:-51165943 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATTAACCAACATCATGGTAT+TGG - chr4_2:51165694-51165713 None:intergenic 30.0%
! AATACCATGATGTTGGTTAA+TGG + chr4_2:51165693-51165712 Msa0605350:CDS 30.0%
TTATGTCACATGACAAAACA+AGG + chr4_2:51165723-51165742 Msa0605350:CDS 30.0%
TATGTCGTTATAAGGATTCA+GGG + chr4_2:51165850-51165869 Msa0605350:CDS 30.0%
ATACAAGCAGGTTATGATTG+TGG + chr4_2:51165650-51165669 Msa0605350:CDS 35.0%
AGTGGTGACAATTCTGTTAA+TGG + chr4_2:51165771-51165790 Msa0605350:CDS 35.0%
TCAATGCCTATGTCGTTATA+AGG + chr4_2:51165842-51165861 Msa0605350:CDS 35.0%
CTGAATCCTTATAACGACAT+AGG - chr4_2:51165851-51165870 None:intergenic 35.0%
CTATGTCGTTATAAGGATTC+AGG + chr4_2:51165849-51165868 Msa0605350:CDS 35.0%
TCCTGTTAACTGTAAGCTTA+TGG + chr4_2:51165917-51165936 Msa0605350:CDS 35.0%
TCCATAAGCTTACAGTTAAC+AGG - chr4_2:51165921-51165940 None:intergenic 35.0%
TATGATTGTGGCGATGTTGT+TGG + chr4_2:51165662-51165681 Msa0605350:CDS 40.0%
!! CAACATCATGGTATTGGCAA+TGG - chr4_2:51165688-51165707 None:intergenic 40.0%
CATTGCCAATACCATGATGT+TGG + chr4_2:51165686-51165705 Msa0605350:CDS 40.0%
CTGACCATTAACCAACATCA+TGG - chr4_2:51165700-51165719 None:intergenic 40.0%
CATGACAAAACAAGGACTGA+AGG + chr4_2:51165731-51165750 Msa0605350:CDS 40.0%
GAATTGTCACCACTCACATA+CGG - chr4_2:51165765-51165784 None:intergenic 40.0%
!! ACGACATAGGCATTGAAGAT+CGG - chr4_2:51165838-51165857 None:intergenic 40.0%
! TCAGGGTTGCTTTCTTTCTA+TGG + chr4_2:51165867-51165886 Msa0605350:CDS 40.0%
TATTGATCCTAACCAAGCCA+TGG + chr4_2:51165890-51165909 Msa0605350:CDS 40.0%
ACAGTTAACAGGAAGTTCCA+TGG - chr4_2:51165910-51165929 None:intergenic 40.0%
! AAACAAGGACTGAAGGCATG+CGG + chr4_2:51165738-51165757 Msa0605350:CDS 45.0%
!! TAGGCATTGAAGATCGGCTT+TGG - chr4_2:51165832-51165851 None:intergenic 45.0%
TAACAGGAAGTTCCATGGCT+TGG - chr4_2:51165905-51165924 None:intergenic 45.0%
! AACAAGGACTGAAGGCATGC+GGG + chr4_2:51165739-51165758 Msa0605350:CDS 50.0%
!! GGAAGTTCCATGGCTTGGTT+AGG - chr4_2:51165900-51165919 None:intergenic 50.0%
! GCCGTCTGATGCTTGCTGCT+CGG + chr4_2:51165800-51165819 Msa0605350:CDS 60.0%
GCCGAGCAGCAAGCATCAGA+CGG - chr4_2:51165804-51165823 None:intergenic 60.0%
GCATGCGGGCCGTATGTGAG+TGG + chr4_2:51165753-51165772 Msa0605350:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 51165642 51165959 51165642 ID=Msa0605350;Name=Msa0605350
chr4_2 mRNA 51165642 51165959 51165642 ID=Msa0605350-mRNA-1;Parent=Msa0605350;Name=Msa0605350-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|105
chr4_2 exon 51165642 51165959 51165642 ID=Msa0605350-mRNA-1:exon:12213;Parent=Msa0605350-mRNA-1
chr4_2 CDS 51165642 51165959 51165642 ID=Msa0605350-mRNA-1:cds;Parent=Msa0605350-mRNA-1
Gene Sequence

>Msa0605350

ATGGAGGCATACAAGCAGGTTATGATTGTGGCGATGTTGTTGGCCATTGCCAATACCATGATGTTGGTTAATGGTCAGTCGTTATGTCACATGACAAAACAAGGACTGAAGGCATGCGGGCCGTATGTGAGTGGTGACAATTCTGTTAATGGTCAAAAGCCGTCTGATGCTTGCTGCTCGGCTATTGCCAAAGCCGATCTTCAATGCCTATGTCGTTATAAGGATTCAGGGTTGCTTTCTTTCTATGGTATTGATCCTAACCAAGCCATGGAACTTCCTGTTAACTGTAAGCTTATGGACTCCTTCCACTGCTACTAG

Protein sequence

>Msa0605350

MEAYKQVMIVAMLLAIANTMMLVNGQSLCHMTKQGLKACGPYVSGDNSVNGQKPSDACCSAIAKADLQCLCRYKDSGLLSFYGIDPNQAMELPVNCKLMDSFHCY*