AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0549870


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0549870 MtrunA17_Chr4g0034581 91.346 104 9 0 1 104 1 104 1.86e-68 200
Msa0549870 MtrunA17_Chr4g0034531 73.077 104 28 0 1 104 1 104 1.13e-52 160
Msa0549870 MtrunA17_Chr4g0034561 55.238 105 45 2 1 104 1 104 2.01e-37 122
Msa0549870 MtrunA17_Chr4g0034571 42.857 105 52 2 1 105 1 97 2.66e-21 84.0
Msa0549870 MtrunA17_Chr7g0234401 41.748 103 57 1 2 104 5 104 8.68e-21 79.7
Msa0549870 MtrunA17_Chr5g0445131 33.654 104 60 4 2 104 7 102 1.83e-11 55.8
Msa0549870 MtrunA17_Chr3g0100281 36.667 90 49 3 7 96 14 95 4.49e-11 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0549870 AT5G48490.1 36.634 101 60 2 5 104 4 101 4.02e-14 63.2
Msa0549870 AT5G48485.1 41.558 77 42 1 28 104 29 102 6.02e-14 62.8

Find 33 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGGGTTGCTTTCTTTCTA+TGG 0.215569 4_1:+45136913 Msa0549870:CDS
CTATGTCGTTATAAGGATTC+AGG 0.249484 4_1:+45136895 Msa0549870:CDS
TCCTGTTAACTGTAAGCTTA+TGG 0.254280 4_1:+45136963 Msa0549870:CDS
TCAATGCCTATGTCGTTATA+AGG 0.293667 4_1:+45136888 Msa0549870:CDS
AATACCATGATGTTGGTTAA+TGG 0.374528 4_1:+45136739 Msa0549870:CDS
AGTGGTGACAATTCTGTTAA+TGG 0.378651 4_1:+45136817 Msa0549870:CDS
TCCATAAGCTTACAGTTAAC+AGG 0.382556 4_1:-45136964 None:intergenic
GGAAGTTCCATGGCTTGGTT+AGG 0.427875 4_1:-45136943 None:intergenic
TAGGCATTGAAGATCGGCTT+TGG 0.464541 4_1:-45136875 None:intergenic
ATTAACCAACATCATGGTAT+TGG 0.470830 4_1:-45136737 None:intergenic
TAACAGGAAGTTCCATGGCT+TGG 0.486774 4_1:-45136948 None:intergenic
TATGATTGTGGCGATGTTGT+TGG 0.507427 4_1:+45136708 Msa0549870:CDS
TATGTCGTTATAAGGATTCA+GGG 0.530226 4_1:+45136896 Msa0549870:CDS
CTGAATCCTTATAACGACAT+AGG 0.550946 4_1:-45136894 None:intergenic
AGAGATGGAGGCATACAAGC+AGG 0.551353 4_1:+45136684 None:intergenic
AACAAGGACTGAAGGCATGC+GGG 0.556452 4_1:+45136785 Msa0549870:CDS
GCCATCCGATGCTTGCTGCT+CGG 0.562907 4_1:+45136846 Msa0549870:CDS
ACAGTTAACAGGAAGTTCCA+TGG 0.568806 4_1:-45136953 None:intergenic
CTGACCATTAACCAACATCA+TGG 0.570185 4_1:-45136743 None:intergenic
GCCGAGCAGCAAGCATCGGA+TGG 0.578399 4_1:-45136847 None:intergenic
ACGACATAGGCATTGAAGAT+CGG 0.583458 4_1:-45136881 None:intergenic
GAATTGTCACCACTCACATA+CGG 0.587779 4_1:-45136808 None:intergenic
CATGACAAAACAAGGACTGA+AGG 0.588836 4_1:+45136777 Msa0549870:CDS
AATAGCCGAGCAGCAAGCAT+CGG 0.592693 4_1:-45136851 None:intergenic
ATACAAGCAGGTTATGATTG+TGG 0.602790 4_1:+45136696 Msa0549870:CDS
CAACATCATGGTATTGGCAA+TGG 0.605291 4_1:-45136731 None:intergenic
CATTGCCAATACCATGATGT+TGG 0.609484 4_1:+45136732 Msa0549870:CDS
TTATGTCACATGACAAAACA+AGG 0.627195 4_1:+45136769 Msa0549870:CDS
AAACAAGGACTGAAGGCATG+CGG 0.631589 4_1:+45136784 Msa0549870:CDS
TATTGATCCTAACCAAGCCA+TGG 0.632800 4_1:+45136936 Msa0549870:CDS
AACCACATGACTAGTAGCAG+TGG 0.639755 4_1:-45136993 None:intergenic
GCATGCGGGCCGTATGTGAG+TGG 0.668512 4_1:+45136799 Msa0549870:CDS
ACATGACTAGTAGCAGTGGA+AGG 0.673463 4_1:-45136989 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATTAACCAACATCATGGTAT+TGG - chr4_1:45136740-45136759 None:intergenic 30.0%
! AATACCATGATGTTGGTTAA+TGG + chr4_1:45136739-45136758 Msa0549870:CDS 30.0%
TTATGTCACATGACAAAACA+AGG + chr4_1:45136769-45136788 Msa0549870:CDS 30.0%
TATGTCGTTATAAGGATTCA+GGG + chr4_1:45136896-45136915 Msa0549870:CDS 30.0%
ATACAAGCAGGTTATGATTG+TGG + chr4_1:45136696-45136715 Msa0549870:CDS 35.0%
AGTGGTGACAATTCTGTTAA+TGG + chr4_1:45136817-45136836 Msa0549870:CDS 35.0%
TCAATGCCTATGTCGTTATA+AGG + chr4_1:45136888-45136907 Msa0549870:CDS 35.0%
CTGAATCCTTATAACGACAT+AGG - chr4_1:45136897-45136916 None:intergenic 35.0%
CTATGTCGTTATAAGGATTC+AGG + chr4_1:45136895-45136914 Msa0549870:CDS 35.0%
TCCTGTTAACTGTAAGCTTA+TGG + chr4_1:45136963-45136982 Msa0549870:CDS 35.0%
TCCATAAGCTTACAGTTAAC+AGG - chr4_1:45136967-45136986 None:intergenic 35.0%
TATGATTGTGGCGATGTTGT+TGG + chr4_1:45136708-45136727 Msa0549870:CDS 40.0%
!! CAACATCATGGTATTGGCAA+TGG - chr4_1:45136734-45136753 None:intergenic 40.0%
CATTGCCAATACCATGATGT+TGG + chr4_1:45136732-45136751 Msa0549870:CDS 40.0%
CTGACCATTAACCAACATCA+TGG - chr4_1:45136746-45136765 None:intergenic 40.0%
CATGACAAAACAAGGACTGA+AGG + chr4_1:45136777-45136796 Msa0549870:CDS 40.0%
GAATTGTCACCACTCACATA+CGG - chr4_1:45136811-45136830 None:intergenic 40.0%
!! ACGACATAGGCATTGAAGAT+CGG - chr4_1:45136884-45136903 None:intergenic 40.0%
! TCAGGGTTGCTTTCTTTCTA+TGG + chr4_1:45136913-45136932 Msa0549870:CDS 40.0%
TATTGATCCTAACCAAGCCA+TGG + chr4_1:45136936-45136955 Msa0549870:CDS 40.0%
ACAGTTAACAGGAAGTTCCA+TGG - chr4_1:45136956-45136975 None:intergenic 40.0%
! AAACAAGGACTGAAGGCATG+CGG + chr4_1:45136784-45136803 Msa0549870:CDS 45.0%
!! TAGGCATTGAAGATCGGCTT+TGG - chr4_1:45136878-45136897 None:intergenic 45.0%
TAACAGGAAGTTCCATGGCT+TGG - chr4_1:45136951-45136970 None:intergenic 45.0%
! AACAAGGACTGAAGGCATGC+GGG + chr4_1:45136785-45136804 Msa0549870:CDS 50.0%
AATAGCCGAGCAGCAAGCAT+CGG - chr4_1:45136854-45136873 None:intergenic 50.0%
!! GGAAGTTCCATGGCTTGGTT+AGG - chr4_1:45136946-45136965 None:intergenic 50.0%
! GCCATCCGATGCTTGCTGCT+CGG + chr4_1:45136846-45136865 Msa0549870:CDS 60.0%
GCATGCGGGCCGTATGTGAG+TGG + chr4_1:45136799-45136818 Msa0549870:CDS 65.0%
!! GCCGAGCAGCAAGCATCGGA+TGG - chr4_1:45136850-45136869 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 45136688 45137005 45136688 ID=Msa0549870;Name=Msa0549870
chr4_1 mRNA 45136688 45137005 45136688 ID=Msa0549870-mRNA-1;Parent=Msa0549870;Name=Msa0549870-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|105
chr4_1 exon 45136688 45137005 45136688 ID=Msa0549870-mRNA-1:exon:11831;Parent=Msa0549870-mRNA-1
chr4_1 CDS 45136688 45137005 45136688 ID=Msa0549870-mRNA-1:cds;Parent=Msa0549870-mRNA-1
Gene Sequence

>Msa0549870

ATGGAGGCATACAAGCAGGTTATGATTGTGGCGATGTTGTTGGCCATTGCCAATACCATGATGTTGGTTAATGGTCAGTCGTTATGTCACATGACAAAACAAGGACTGAAGGCATGCGGGCCGTATGTGAGTGGTGACAATTCTGTTAATGGTCAAAAGCCATCCGATGCTTGCTGCTCGGCTATTGCCAAAGCCGATCTTCAATGCCTATGTCGTTATAAGGATTCAGGGTTGCTTTCTTTCTATGGTATTGATCCTAACCAAGCCATGGAACTTCCTGTTAACTGTAAGCTTATGGACTCCTTCCACTGCTACTAG

Protein sequence

>Msa0549870

MEAYKQVMIVAMLLAIANTMMLVNGQSLCHMTKQGLKACGPYVSGDNSVNGQKPSDACCSAIAKADLQCLCRYKDSGLLSFYGIDPNQAMELPVNCKLMDSFHCY*