AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0646260


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0646260 MtrunA17_Chr4g0034581 91.346 104 9 0 1 104 1 104 1.86e-68 200
Msa0646260 MtrunA17_Chr4g0034531 73.077 104 28 0 1 104 1 104 1.13e-52 160
Msa0646260 MtrunA17_Chr4g0034561 55.238 105 45 2 1 104 1 104 2.01e-37 122
Msa0646260 MtrunA17_Chr4g0034571 42.857 105 52 2 1 105 1 97 2.66e-21 84.0
Msa0646260 MtrunA17_Chr7g0234401 41.748 103 57 1 2 104 5 104 8.68e-21 79.7
Msa0646260 MtrunA17_Chr5g0445131 33.654 104 60 4 2 104 7 102 1.83e-11 55.8
Msa0646260 MtrunA17_Chr3g0100281 36.667 90 49 3 7 96 14 95 4.49e-11 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0646260 AT5G48490.1 36.634 101 60 2 5 104 4 101 4.02e-14 63.2
Msa0646260 AT5G48485.1 41.558 77 42 1 28 104 29 102 6.02e-14 62.8

Find 31 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGGGTTGCTTTCTTTCTA+TGG 0.215569 4_3:-51902328 Msa0646260:CDS
CTATGTCGTTATAAGGATTC+AGG 0.249484 4_3:-51902346 Msa0646260:CDS
TCCTGTTAACTGTAAGCTTA+TGG 0.254280 4_3:-51902278 Msa0646260:CDS
TCAATGCCTATGTCGTTATA+AGG 0.293667 4_3:-51902353 Msa0646260:CDS
TCCATAAGCTTACAGTTAAC+AGG 0.370185 4_3:+51902277 None:intergenic
AATACCATGATGTTGGTTAA+TGG 0.374528 4_3:-51902502 Msa0646260:CDS
AGTGGTGACAATTCTGTTAA+TGG 0.378651 4_3:-51902424 Msa0646260:CDS
GGAAGTTCCATGGCTTGGTT+AGG 0.427875 4_3:+51902298 None:intergenic
TAGGCATTGAAGATCGGCTT+TGG 0.464541 4_3:+51902366 None:intergenic
ATTAACCAACATCATGGTAT+TGG 0.470830 4_3:+51902504 None:intergenic
TAACAGGAAGTTCCATGGCT+TGG 0.486774 4_3:+51902293 None:intergenic
TATGATTGTGGCGATGTTGT+TGG 0.507427 4_3:-51902533 Msa0646260:CDS
TATGTCGTTATAAGGATTCA+GGG 0.530226 4_3:-51902345 Msa0646260:CDS
CTGAATCCTTATAACGACAT+AGG 0.550946 4_3:+51902347 None:intergenic
AGAGATGGAGGCATACAAGC+AGG 0.551353 4_3:-51902557 None:intergenic
AACAAGGACTGAAGGCATGC+GGG 0.556452 4_3:-51902456 Msa0646260:CDS
GCCGTCTGATGCTTGCTGCT+CGG 0.561297 4_3:-51902395 Msa0646260:CDS
ACAGTTAACAGGAAGTTCCA+TGG 0.568806 4_3:+51902288 None:intergenic
CTGACCATTAACCAACATCA+TGG 0.570185 4_3:+51902498 None:intergenic
GCCGAGCAGCAAGCATCAGA+CGG 0.582886 4_3:+51902394 None:intergenic
ACGACATAGGCATTGAAGAT+CGG 0.583458 4_3:+51902360 None:intergenic
GAATTGTCACCACTCACATA+CGG 0.587779 4_3:+51902433 None:intergenic
CATGACAAAACAAGGACTGA+AGG 0.588836 4_3:-51902464 Msa0646260:CDS
ATACAAGCAGGTTATGATTG+TGG 0.602790 4_3:-51902545 Msa0646260:CDS
CAACATCATGGTATTGGCAA+TGG 0.605291 4_3:+51902510 None:intergenic
CATTGCCAATACCATGATGT+TGG 0.609484 4_3:-51902509 Msa0646260:CDS
TTATGTCACATGACAAAACA+AGG 0.627195 4_3:-51902472 Msa0646260:CDS
AAACAAGGACTGAAGGCATG+CGG 0.631589 4_3:-51902457 Msa0646260:CDS
TATTGATCCTAACCAAGCCA+TGG 0.632800 4_3:-51902305 Msa0646260:CDS
AACCACATGACTAGTAGCAG+TGG 0.639755 4_3:+51902248 None:intergenic
GCATGCGGGCCGTATGTGAG+TGG 0.668512 4_3:-51902442 Msa0646260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATTAACCAACATCATGGTAT+TGG + chr4_3:51902310-51902329 None:intergenic 30.0%
! AATACCATGATGTTGGTTAA+TGG - chr4_3:51902309-51902328 Msa0646260:CDS 30.0%
TTATGTCACATGACAAAACA+AGG - chr4_3:51902339-51902358 Msa0646260:CDS 30.0%
TATGTCGTTATAAGGATTCA+GGG - chr4_3:51902466-51902485 Msa0646260:CDS 30.0%
ATACAAGCAGGTTATGATTG+TGG - chr4_3:51902266-51902285 Msa0646260:CDS 35.0%
AGTGGTGACAATTCTGTTAA+TGG - chr4_3:51902387-51902406 Msa0646260:CDS 35.0%
TCAATGCCTATGTCGTTATA+AGG - chr4_3:51902458-51902477 Msa0646260:CDS 35.0%
CTGAATCCTTATAACGACAT+AGG + chr4_3:51902467-51902486 None:intergenic 35.0%
CTATGTCGTTATAAGGATTC+AGG - chr4_3:51902465-51902484 Msa0646260:CDS 35.0%
TCCTGTTAACTGTAAGCTTA+TGG - chr4_3:51902533-51902552 Msa0646260:CDS 35.0%
TCCATAAGCTTACAGTTAAC+AGG + chr4_3:51902537-51902556 None:intergenic 35.0%
TATGATTGTGGCGATGTTGT+TGG - chr4_3:51902278-51902297 Msa0646260:CDS 40.0%
!! CAACATCATGGTATTGGCAA+TGG + chr4_3:51902304-51902323 None:intergenic 40.0%
CATTGCCAATACCATGATGT+TGG - chr4_3:51902302-51902321 Msa0646260:CDS 40.0%
CTGACCATTAACCAACATCA+TGG + chr4_3:51902316-51902335 None:intergenic 40.0%
CATGACAAAACAAGGACTGA+AGG - chr4_3:51902347-51902366 Msa0646260:CDS 40.0%
GAATTGTCACCACTCACATA+CGG + chr4_3:51902381-51902400 None:intergenic 40.0%
!! ACGACATAGGCATTGAAGAT+CGG + chr4_3:51902454-51902473 None:intergenic 40.0%
! TCAGGGTTGCTTTCTTTCTA+TGG - chr4_3:51902483-51902502 Msa0646260:CDS 40.0%
TATTGATCCTAACCAAGCCA+TGG - chr4_3:51902506-51902525 Msa0646260:CDS 40.0%
ACAGTTAACAGGAAGTTCCA+TGG + chr4_3:51902526-51902545 None:intergenic 40.0%
! AAACAAGGACTGAAGGCATG+CGG - chr4_3:51902354-51902373 Msa0646260:CDS 45.0%
!! TAGGCATTGAAGATCGGCTT+TGG + chr4_3:51902448-51902467 None:intergenic 45.0%
TAACAGGAAGTTCCATGGCT+TGG + chr4_3:51902521-51902540 None:intergenic 45.0%
! AACAAGGACTGAAGGCATGC+GGG - chr4_3:51902355-51902374 Msa0646260:CDS 50.0%
!! GGAAGTTCCATGGCTTGGTT+AGG + chr4_3:51902516-51902535 None:intergenic 50.0%
! GCCGTCTGATGCTTGCTGCT+CGG - chr4_3:51902416-51902435 Msa0646260:CDS 60.0%
GCCGAGCAGCAAGCATCAGA+CGG + chr4_3:51902420-51902439 None:intergenic 60.0%
GCATGCGGGCCGTATGTGAG+TGG - chr4_3:51902369-51902388 Msa0646260:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 51902258 51902575 51902258 ID=Msa0646260;Name=Msa0646260
chr4_3 mRNA 51902258 51902575 51902258 ID=Msa0646260-mRNA-1;Parent=Msa0646260;Name=Msa0646260-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|105
chr4_3 exon 51902258 51902575 51902258 ID=Msa0646260-mRNA-1:exon:12651;Parent=Msa0646260-mRNA-1
chr4_3 CDS 51902258 51902575 51902258 ID=Msa0646260-mRNA-1:cds;Parent=Msa0646260-mRNA-1
Gene Sequence

>Msa0646260

ATGGAGGCATACAAGCAGGTTATGATTGTGGCGATGTTGTTGGCCATTGCCAATACCATGATGTTGGTTAATGGTCAGTCGTTATGTCACATGACAAAACAAGGACTGAAGGCATGCGGGCCGTATGTGAGTGGTGACAATTCTGTTAATGGTCAAAAGCCGTCTGATGCTTGCTGCTCGGCTATTGCCAAAGCCGATCTTCAATGCCTATGTCGTTATAAGGATTCAGGGTTGCTTTCTTTCTATGGTATTGATCCTAACCAAGCCATGGAACTTCCTGTTAACTGTAAGCTTATGGACTCATTCCACTGCTACTAG

Protein sequence

>Msa0646260

MEAYKQVMIVAMLLAIANTMMLVNGQSLCHMTKQGLKACGPYVSGDNSVNGQKPSDACCSAIAKADLQCLCRYKDSGLLSFYGIDPNQAMELPVNCKLMDSFHCY*