AlfalfaGEDB | MS.gene38348

M. sativa cultivar XinJiangDaYe / MS.gene38348

KEGG: K01623
GO: GO:0003674 GO:0003824 GO:0004332 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006165 GO:0006725 GO:0006753 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009117 GO:0009132 GO:0009135 GO:0009150 GO:0009179 GO:0009185 GO:0009259 GO:0009987 GO:0016052 GO:0016310 GO:0016829 GO:0016830 GO:0016832 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034641 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0046031 GO:0046034 GO:0046483 GO:0046939 GO:0055086 GO:0071704 GO:0072521 GO:1901135 GO:1901360 GO:1901564 GO:1901575
PFAM: PF00274.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38348.t1	XP_004491539.1	98.7	237	3	0	1	237	1	237	3.40E-128	467.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38348.t1	P46256	97.9	237	5	0	1	237	1	237	2.2e-128	459.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38348.t1	A0A1S2XMC3	98.7	237	3	0	1	237	1	237	2.4e-128	467.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene38348	0.921428	3.63E-88	-1.69E-46
MS.gene049173	MS.gene38348	0.826619	2.46E-54	-1.69E-46
MS.gene049217	MS.gene38348	0.828508	8.65E-55	-1.69E-46
MS.gene049222	MS.gene38348	0.867338	1.47E-65	-1.69E-46
MS.gene049232	MS.gene38348	0.81774	2.82E-52	-1.69E-46
MS.gene049281	MS.gene38348	-0.825499	4.54E-54	-1.69E-46
MS.gene049285	MS.gene38348	0.912909	1.13E-83	-1.69E-46
MS.gene049320	MS.gene38348	0.91241	2.01E-83	-1.69E-46
MS.gene04939	MS.gene38348	0.810967	8.86E-51	-1.69E-46
MS.gene049609	MS.gene38348	0.857864	1.22E-62	-1.69E-46
MS.gene049633	MS.gene38348	0.867576	1.24E-65	-1.69E-46
MS.gene049865	MS.gene38348	0.806437	8.23E-50	-1.69E-46
MS.gene05010	MS.gene38348	0.873726	1.18E-67	-1.69E-46
MS.gene050163	MS.gene38348	0.867412	1.40E-65	-1.69E-46
MS.gene050165	MS.gene38348	0.865966	4.02E-65	-1.69E-46
MS.gene050170	MS.gene38348	0.881619	2.07E-70	-1.69E-46
MS.gene050323	MS.gene38348	0.869416	3.15E-66	-1.69E-46
MS.gene050333	MS.gene38348	0.840461	8.58E-58	-1.69E-46
MS.gene050444	MS.gene38348	0.816204	6.24E-52	-1.69E-46
MS.gene050496	MS.gene38348	0.891862	2.70E-74	-1.69E-46
MS.gene050504	MS.gene38348	0.907166	6.80E-81	-1.69E-46
MS.gene050518	MS.gene38348	0.883952	2.90E-71	-1.69E-46
MS.gene050577	MS.gene38348	0.910213	2.41E-82	-1.69E-46
MS.gene050612	MS.gene38348	0.910555	1.64E-82	-1.69E-46
MS.gene050675	MS.gene38348	0.877387	6.55E-69	-1.69E-46
MS.gene050699	MS.gene38348	0.938119	1.14E-98	-1.69E-46
MS.gene050747	MS.gene38348	0.832067	1.17E-55	-1.69E-46
MS.gene050772	MS.gene38348	0.881576	2.14E-70	-1.69E-46
MS.gene05080	MS.gene38348	0.838295	3.13E-57	-1.69E-46
MS.gene05081	MS.gene38348	0.804114	2.52E-49	-1.69E-46
MS.gene050868	MS.gene38348	0.841557	4.42E-58	-1.69E-46
MS.gene050871	MS.gene38348	0.837053	6.52E-57	-1.69E-46
MS.gene050876	MS.gene38348	0.835884	1.29E-56	-1.69E-46
MS.gene050904	MS.gene38348	0.836703	8.01E-57	-1.69E-46
MS.gene050950	MS.gene38348	0.828383	9.27E-55	-1.69E-46
MS.gene050986	MS.gene38348	0.903465	3.37E-79	-1.69E-46
MS.gene05100	MS.gene38348	0.842977	1.86E-58	-1.69E-46
MS.gene051317	MS.gene38348	0.887178	1.79E-72	-1.69E-46
MS.gene051336	MS.gene38348	0.820663	6.09E-53	-1.69E-46
MS.gene051364	MS.gene38348	-0.818023	2.43E-52	-1.69E-46
MS.gene051366	MS.gene38348	-0.814408	1.56E-51	-1.69E-46
MS.gene051551	MS.gene38348	0.881156	3.03E-70	-1.69E-46
MS.gene051651	MS.gene38348	-0.820337	7.24E-53	-1.69E-46
MS.gene051810	MS.gene38348	0.874209	8.07E-68	-1.69E-46
MS.gene051838	MS.gene38348	0.823407	1.41E-53	-1.69E-46
MS.gene051858	MS.gene38348	0.818685	1.72E-52	-1.69E-46
MS.gene051949	MS.gene38348	0.833491	5.17E-56	-1.69E-46
MS.gene052164	MS.gene38348	0.83852	2.74E-57	-1.69E-46
MS.gene052271	MS.gene38348	0.80731	5.38E-50	-1.69E-46
MS.gene052482	MS.gene38348	0.864595	1.09E-64	-1.69E-46
MS.gene052486	MS.gene38348	0.874061	9.06E-68	-1.69E-46
MS.gene052501	MS.gene38348	0.800836	1.20E-48	-1.69E-46
MS.gene052503	MS.gene38348	0.88246	1.02E-70	-1.69E-46
MS.gene052504	MS.gene38348	0.811182	7.96E-51	-1.69E-46
MS.gene052506	MS.gene38348	0.856231	3.70E-62	-1.69E-46
MS.gene052615	MS.gene38348	0.849151	3.89E-60	-1.69E-46
MS.gene052822	MS.gene38348	0.866527	2.67E-65	-1.69E-46
MS.gene052823	MS.gene38348	0.877633	5.37E-69	-1.69E-46
MS.gene052825	MS.gene38348	0.87517	3.81E-68	-1.69E-46
MS.gene052872	MS.gene38348	0.817129	3.87E-52	-1.69E-46
MS.gene052916	MS.gene38348	0.805937	1.05E-49	-1.69E-46
MS.gene052937	MS.gene38348	-0.805745	1.15E-49	-1.69E-46
MS.gene052966	MS.gene38348	0.818031	2.42E-52	-1.69E-46
MS.gene053203	MS.gene38348	0.867227	1.60E-65	-1.69E-46
MS.gene053207	MS.gene38348	0.815079	1.11E-51	-1.69E-46
MS.gene053213	MS.gene38348	0.879537	1.15E-69	-1.69E-46
MS.gene053286	MS.gene38348	0.879502	1.18E-69	-1.69E-46
MS.gene053436	MS.gene38348	0.839902	1.20E-57	-1.69E-46
MS.gene053479	MS.gene38348	0.842112	3.15E-58	-1.69E-46
MS.gene053480	MS.gene38348	0.882075	1.41E-70	-1.69E-46
MS.gene053574	MS.gene38348	0.842792	2.08E-58	-1.69E-46
MS.gene053632	MS.gene38348	0.861513	9.74E-64	-1.69E-46
MS.gene053824	MS.gene38348	0.866249	3.27E-65	-1.69E-46
MS.gene053916	MS.gene38348	0.820274	7.48E-53	-1.69E-46
MS.gene054343	MS.gene38348	0.838014	3.70E-57	-1.69E-46
MS.gene054344	MS.gene38348	0.863183	2.99E-64	-1.69E-46
MS.gene054408	MS.gene38348	0.819464	1.15E-52	-1.69E-46
MS.gene054555	MS.gene38348	-0.808636	2.81E-50	-1.69E-46
MS.gene054595	MS.gene38348	0.829403	5.25E-55	-1.69E-46
MS.gene054600	MS.gene38348	0.87461	5.90E-68	-1.69E-46
MS.gene054642	MS.gene38348	0.887769	1.07E-72	-1.69E-46
MS.gene05470	MS.gene38348	0.897299	1.61E-76	-1.69E-46
MS.gene054954	MS.gene38348	0.917721	3.76E-86	-1.69E-46
MS.gene054959	MS.gene38348	-0.830205	3.35E-55	-1.69E-46
MS.gene055134	MS.gene38348	-0.816786	4.62E-52	-1.69E-46
MS.gene055154	MS.gene38348	-0.804657	1.94E-49	-1.69E-46
MS.gene055206	MS.gene38348	0.842945	1.90E-58	-1.69E-46
MS.gene055251	MS.gene38348	-0.840829	6.87E-58	-1.69E-46
MS.gene055362	MS.gene38348	0.923177	3.74E-89	-1.69E-46
MS.gene055502	MS.gene38348	0.903176	4.54E-79	-1.69E-46
MS.gene055577	MS.gene38348	0.879259	1.44E-69	-1.69E-46
MS.gene055719	MS.gene38348	-0.810745	9.89E-51	-1.69E-46
MS.gene055722	MS.gene38348	0.826532	2.58E-54	-1.69E-46
MS.gene055748	MS.gene38348	0.871469	6.68E-67	-1.69E-46
MS.gene055750	MS.gene38348	0.865496	5.66E-65	-1.69E-46
MS.gene055751	MS.gene38348	0.878505	2.66E-69	-1.69E-46
MS.gene055776	MS.gene38348	0.883115	5.90E-71	-1.69E-46
MS.gene055849	MS.gene38348	0.810251	1.26E-50	-1.69E-46
MS.gene05589	MS.gene38348	0.888959	3.72E-73	-1.69E-46
MS.gene055941	MS.gene38348	0.831747	1.40E-55	-1.69E-46
MS.gene055942	MS.gene38348	-0.818334	2.07E-52	-1.69E-46
MS.gene055956	MS.gene38348	-0.823539	1.31E-53	-1.69E-46
MS.gene056046	MS.gene38348	0.816711	4.80E-52	-1.69E-46
MS.gene056083	MS.gene38348	0.804559	2.04E-49	-1.69E-46
MS.gene056266	MS.gene38348	0.805869	1.08E-49	-1.69E-46
MS.gene056374	MS.gene38348	0.842653	2.27E-58	-1.69E-46
MS.gene056409	MS.gene38348	0.800948	1.13E-48	-1.69E-46
MS.gene056446	MS.gene38348	0.805091	1.58E-49	-1.69E-46
MS.gene056504	MS.gene38348	0.913761	4.23E-84	-1.69E-46
MS.gene056510	MS.gene38348	0.88609	4.63E-72	-1.69E-46
MS.gene056550	MS.gene38348	-0.805055	1.61E-49	-1.69E-46
MS.gene056610	MS.gene38348	0.823817	1.13E-53	-1.69E-46
MS.gene056657	MS.gene38348	0.908907	1.02E-81	-1.69E-46
MS.gene056692	MS.gene38348	0.869178	3.76E-66	-1.69E-46
MS.gene056761	MS.gene38348	-0.81183	5.75E-51	-1.69E-46
MS.gene056898	MS.gene38348	0.807216	5.63E-50	-1.69E-46
MS.gene057076	MS.gene38348	0.824309	8.66E-54	-1.69E-46
MS.gene057196	MS.gene38348	0.814769	1.30E-51	-1.69E-46
MS.gene057331	MS.gene38348	0.811505	6.77E-51	-1.69E-46
MS.gene057332	MS.gene38348	0.835408	1.71E-56	-1.69E-46
MS.gene057416	MS.gene38348	0.835072	2.08E-56	-1.69E-46
MS.gene057658	MS.gene38348	0.854797	9.69E-62	-1.69E-46
MS.gene057831	MS.gene38348	0.855839	4.82E-62	-1.69E-46
MS.gene058334	MS.gene38348	-0.866024	3.86E-65	-1.69E-46
MS.gene058338	MS.gene38348	0.849778	2.60E-60	-1.69E-46
MS.gene05837	MS.gene38348	0.848474	5.99E-60	-1.69E-46
MS.gene058382	MS.gene38348	-0.808759	2.64E-50	-1.69E-46
MS.gene058419	MS.gene38348	0.928731	1.91E-92	-1.69E-46
MS.gene058544	MS.gene38348	0.888554	5.33E-73	-1.69E-46
MS.gene058719	MS.gene38348	0.800744	1.25E-48	-1.69E-46
MS.gene058723	MS.gene38348	0.870238	1.70E-66	-1.69E-46
MS.gene058773	MS.gene38348	0.857481	1.58E-62	-1.69E-46
MS.gene058838	MS.gene38348	0.880862	3.87E-70	-1.69E-46
MS.gene059022	MS.gene38348	0.917566	4.54E-86	-1.69E-46
MS.gene059023	MS.gene38348	0.908367	1.85E-81	-1.69E-46
MS.gene05912	MS.gene38348	0.811673	6.22E-51	-1.69E-46
MS.gene05913	MS.gene38348	0.809027	2.32E-50	-1.69E-46
MS.gene05914	MS.gene38348	0.841854	3.69E-58	-1.69E-46
MS.gene05934	MS.gene38348	0.804718	1.89E-49	-1.69E-46
MS.gene059667	MS.gene38348	0.810968	8.85E-51	-1.69E-46
MS.gene059677	MS.gene38348	0.930676	1.16E-93	-1.69E-46
MS.gene059718	MS.gene38348	0.877153	7.90E-69	-1.69E-46
MS.gene059756	MS.gene38348	0.900694	5.67E-78	-1.69E-46
MS.gene059770	MS.gene38348	-0.829442	5.14E-55	-1.69E-46
MS.gene059801	MS.gene38348	0.801646	8.16E-49	-1.69E-46
MS.gene059837	MS.gene38348	0.871762	5.34E-67	-1.69E-46
MS.gene059865	MS.gene38348	0.889164	3.09E-73	-1.69E-46
MS.gene059902	MS.gene38348	0.814068	1.86E-51	-1.69E-46
MS.gene06000	MS.gene38348	0.841913	3.56E-58	-1.69E-46
MS.gene060337	MS.gene38348	-0.811473	6.88E-51	-1.69E-46
MS.gene060703	MS.gene38348	0.891049	5.66E-74	-1.69E-46
MS.gene060728	MS.gene38348	0.80879	2.60E-50	-1.69E-46
MS.gene060762	MS.gene38348	0.872265	3.63E-67	-1.69E-46
MS.gene060778	MS.gene38348	0.840298	9.46E-58	-1.69E-46
MS.gene06077	MS.gene38348	0.912061	3.00E-83	-1.69E-46
MS.gene060835	MS.gene38348	0.806373	8.49E-50	-1.69E-46
MS.gene06092	MS.gene38348	0.847751	9.49E-60	-1.69E-46
MS.gene060982	MS.gene38348	0.903152	4.66E-79	-1.69E-46
MS.gene061044	MS.gene38348	-0.829128	6.12E-55	-1.69E-46
MS.gene061150	MS.gene38348	0.874304	7.50E-68	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38348.t1	MTR_5g096670	98.734	237	3	0	1	237	1	237	1.50e-173	481
MS.gene38348.t1	MTR_5g069055	81.857	237	43	0	1	237	1	237	1.75e-145	410
MS.gene38348.t1	MTR_1g061050	80.169	237	47	0	1	237	1	237	6.96e-141	399
MS.gene38348.t1	MTR_5g069050	76.754	228	38	1	10	237	51	263	1.05e-125	361
MS.gene38348.t1	MTR_4g057670	59.565	230	92	1	6	235	51	279	7.97e-93	278
MS.gene38348.t1	MTR_4g071860	59.565	230	92	1	6	235	51	279	8.42e-93	278
MS.gene38348.t1	MTR_1g109200	57.269	227	96	1	8	234	44	269	1.85e-90	272
MS.gene38348.t1	MTR_4g071880	50.644	233	96	3	6	235	49	265	2.25e-71	223
MS.gene38348.t1	MTR_7g103920	64.583	96	9	1	112	207	11	81	6.12e-35	120

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38348.t1	AT4G26530	89.451	237	25	0	1	237	1	237	8.30e-157	439
MS.gene38348.t1	AT4G26530	89.451	237	25	0	1	237	1	237	8.30e-157	439
MS.gene38348.t1	AT4G26530	89.451	237	25	0	1	237	1	237	8.30e-157	439
MS.gene38348.t1	AT3G52930	83.122	237	40	0	1	237	1	237	1.92e-144	408
MS.gene38348.t1	AT2G36460	81.435	237	44	0	1	237	1	237	3.42e-143	405
MS.gene38348.t1	AT4G26520	82.700	237	41	0	1	237	1	237	2.30e-142	402
MS.gene38348.t1	AT4G26520	82.700	237	41	0	1	237	1	237	2.53e-142	402
MS.gene38348.t1	AT4G26520	82.533	229	40	0	9	237	6	234	3.98e-137	389
MS.gene38348.t1	AT4G26520	82.533	229	40	0	9	237	6	234	3.98e-137	389
MS.gene38348.t1	AT5G03690	79.149	235	49	0	1	235	1	235	2.38e-131	375
MS.gene38348.t1	AT5G03690	80.000	225	45	0	11	235	45	269	4.66e-126	362
MS.gene38348.t1	AT4G38970	59.211	228	92	1	8	235	51	277	6.10e-93	278
MS.gene38348.t1	AT4G38970	59.211	228	92	1	8	235	51	277	7.78e-93	278
MS.gene38348.t1	AT2G21330	57.895	228	95	1	8	235	52	278	5.04e-92	273
MS.gene38348.t1	AT2G01140	57.642	229	96	1	6	234	42	269	7.60e-91	273
MS.gene38348.t1	AT2G21330	57.895	228	95	1	8	235	52	278	8.94e-91	273
MS.gene38348.t1	AT2G21330	57.895	228	95	1	8	235	52	278	1.17e-90	272
MS.gene38348.t1	AT2G36460	81.507	146	27	0	92	237	1	146	9.81e-86	256
MS.gene38348.t1	AT2G36460	81.507	146	27	0	92	237	1	146	9.81e-86	256
MS.gene38348.t1	AT5G03690	81.818	143	26	0	93	235	1	143	7.93e-76	230
MS.gene38348.t1	AT5G03690	81.818	143	26	0	93	235	1	143	7.93e-76	230

Find 82 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTTCCCATCGGAGGAATTC+TGG	0.334173	5.3:-78043517	None:intergenic
TTCAGTGACAGCAGCGCATT+TGG	0.358382	5.3:-78043861	None:intergenic
GACAACTCAGAGGGCTCATT+GGG	0.363446	5.3:-78043728	None:intergenic
ATCATTCAGTGCCTTGTAAC+AGG	0.375839	5.3:-78043897	None:intergenic
GGATGACACCGGAGAGGTAT+TGG	0.377053	5.3:-78043475	None:intergenic
GCCAGGGGTGGCTATGTACT+TGG	0.377454	5.3:-78043324	None:intergenic
CCAGCAAAACGCACAGGGCT+TGG	0.395404	5.3:+78043754	MS.gene38348:CDS
CTGGTATGACATTGTTCTCT+TGG	0.404955	5.3:-78043556	None:intergenic
CCCTTGTCAACTTTGATGCC+TGG	0.408200	5.3:-78043575	None:intergenic
AAAATCTCAGGCTCAACAAT+AGG	0.410743	5.3:-78043815	None:intergenic
CACCCCTGGCAAGGGTATCT+TGG	0.410912	5.3:+78043337	MS.gene38348:CDS
GTATGACATTGTTCTCTTGG+AGG	0.415484	5.3:-78043553	None:intergenic
CTCCGGCCTTGTAGTACTGC+TGG	0.419506	5.3:-78043667	None:intergenic
GAACCTGGAGTAACCATGTT+AGG	0.426285	5.3:-78043953	None:intergenic
CTCCGGTGTCATCCTCTTTG+AGG	0.427295	5.3:+78043484	MS.gene38348:CDS
TCTTGGAGGATTTCAACAAA+AGG	0.428975	5.3:-78043539	None:intergenic
GATCCATCGGTTAAAATCTC+AGG	0.430763	5.3:-78043827	None:intergenic
CAAAGCATGTCGGCCTTTGT+TGG	0.432883	5.3:+78042890	None:intergenic
AGACAACTCAGAGGGCTCAT+TGG	0.443250	5.3:-78043729	None:intergenic
GATCAGCATGTCCTTCTTGA+AGG	0.445985	5.3:+78043917	MS.gene38348:CDS
GAGAGGTATTGGAGTGCATT+GGG	0.455590	5.3:-78043464	None:intergenic
CTTGAGTACCGCACGCCACT+TGG	0.484408	5.3:-78043699	None:intergenic
GCCTTTGTTGGAAAGTATGC+AGG	0.487704	5.3:+78042902	MS.gene38348:CDS
AAGGGTGTTGTTGAATTAGC+TGG	0.490593	5.3:+78043593	MS.gene38348:CDS
GCTGCCAAGATACCCTTGCC+AGG	0.495182	5.3:-78043341	None:intergenic
TTCACGGAGGGCTTGACGGT+TGG	0.495893	5.3:-78043426	None:intergenic
GTCTATCCAGCAAAACGCAC+AGG	0.499555	5.3:+78043748	MS.gene38348:CDS
GAAGTTCACGGAGGGCTTGA+CGG	0.504237	5.3:-78043430	None:intergenic
TCCAAGAGAGTCAAAACCTT+GGG	0.506085	5.3:-78043636	None:intergenic
GCCATTCTCCTGGCAAATGA+TGG	0.507357	5.3:-78043786	None:intergenic
TGGCGTGCGGTACTCAAGAT+TGG	0.507577	5.3:+78043704	MS.gene38348:CDS
GATGAGAGCACTGGAACCAT+TGG	0.508526	5.3:+78043365	MS.gene38348:CDS
GTTGAATTAGCTGGAACAAA+TGG	0.509964	5.3:+78043602	MS.gene38348:CDS
ACCTTTGGGCTATCAGAACC+TGG	0.510328	5.3:-78043968	None:intergenic
GCCATCATTTGCCAGGAGAA+TGG	0.511001	5.3:+78043785	MS.gene38348:CDS
GGAGAGGTATTGGAGTGCAT+TGG	0.512442	5.3:-78043465	None:intergenic
CTGCCAAGATACCCTTGCCA+GGG	0.516454	5.3:-78043340	None:intergenic
TAGGCACAAGGCCATTCTCC+TGG	0.518706	5.3:-78043796	None:intergenic
TCGCTACGCCATCATTTGCC+AGG	0.522141	5.3:+78043778	MS.gene38348:CDS
GTATTGGAGTGCATTGGGAG+AGG	0.525633	5.3:-78043459	None:intergenic
GAGAGGTGAAGAGAAGTTCA+CGG	0.536141	5.3:-78043442	None:intergenic
CCACTTGGCAAACCGTGCTC+CGG	0.542305	5.3:-78043684	None:intergenic
GGCGATGTCATGAGATCCAT+CGG	0.542710	5.3:-78043840	None:intergenic
TGAGAAGAGTTCCTTCAAGA+AGG	0.549810	5.3:-78043928	None:intergenic
TACATAGCCACCCCTGGCAA+GGG	0.556199	5.3:+78043329	MS.gene38348:CDS
GGTAAAGAGTTTCCTCAAAG+AGG	0.556676	5.3:-78043496	None:intergenic
CAAGAGAACAATGTCATACC+AGG	0.556695	5.3:+78043557	MS.gene38348:CDS
GCTTGCTAGACGCTTGCCAA+TGG	0.565429	5.3:-78043381	None:intergenic
AAGCCTAACATGGTTACTCC+AGG	0.566895	5.3:+78043950	MS.gene38348:CDS
CTCCAAGAGAGTCAAAACCT+TGG	0.567895	5.3:-78043637	None:intergenic
AATGCACTCCAATACCTCTC+CGG	0.571465	5.3:+78043467	MS.gene38348:CDS
GTACATAGCCACCCCTGGCA+AGG	0.572440	5.3:+78043328	MS.gene38348:CDS
GCCAAGTACATAGCCACCCC+TGG	0.573393	5.3:+78043323	MS.gene38348:CDS
TGCCAGCAGTACTACAAGGC+CGG	0.573670	5.3:+78043665	MS.gene38348:CDS
TGTGCTTGCAGCCTGTTACA+AGG	0.574695	5.3:+78043886	MS.gene38348:CDS
AACAAAAGGCTTCCCATCGG+AGG	0.574707	5.3:-78043525	None:intergenic
AACTCTTCTCAAGCCTAACA+TGG	0.582067	5.3:+78043940	MS.gene38348:CDS
TTGGCAGCAGATGAGAGCAC+TGG	0.583087	5.3:+78043356	MS.gene38348:CDS
CAAGATACCCTTGCCAGGGG+TGG	0.589488	5.3:-78043336	None:intergenic
ACCAGGCATCAAAGTTGACA+AGG	0.595336	5.3:+78043574	MS.gene38348:CDS
CTTTACCAGAATTCCTCCGA+TGG	0.596117	5.3:+78043512	MS.gene38348:CDS
ACCTGCATACTTTCCAACAA+AGG	0.607177	5.3:-78042903	None:intergenic
TTTACCAGAATTCCTCCGAT+GGG	0.613347	5.3:+78043513	MS.gene38348:CDS
TGCTGGATAGACAACTCAGA+GGG	0.616996	5.3:-78043737	None:intergenic
GGTGAAGAGAAGTTCACGGA+GGG	0.617088	5.3:-78043438	None:intergenic
CCGGAGCACGGTTTGCCAAG+TGG	0.620912	5.3:+78043684	MS.gene38348:CDS
TTCAACAAAAGGCTTCCCAT+CGG	0.623890	5.3:-78043528	None:intergenic
TGCCAAGATACCCTTGCCAG+GGG	0.625578	5.3:-78043339	None:intergenic
TAGATGCCAGCAGTACTACA+AGG	0.629007	5.3:+78043661	MS.gene38348:CDS
ACGGTTTGCCAAGTGGCGTG+CGG	0.639426	5.3:+78043691	MS.gene38348:CDS
AATGGTGAAACAACAACCCA+AGG	0.641211	5.3:+78043620	MS.gene38348:CDS
AGTACTACAAGGCCGGAGCA+CGG	0.644656	5.3:+78043672	MS.gene38348:CDS
CAGGCTCAACAATAGGCACA+AGG	0.650137	5.3:-78043808	None:intergenic
TCTATCCAGCAAAACGCACA+GGG	0.656184	5.3:+78043749	MS.gene38348:CDS
TCCAGGTTCTGATAGCCCAA+AGG	0.674535	5.3:+78043967	MS.gene38348:CDS
TTCCTCAAAGAGGATGACAC+CGG	0.677291	5.3:-78043486	None:intergenic
CATCAACGTTGAGAACATTG+AGG	0.688570	5.3:+78043403	MS.gene38348:CDS
CAAAGAGGATGACACCGGAG+AGG	0.703503	5.3:-78043481	None:intergenic
CCAGGCATCAAAGTTGACAA+GGG	0.714216	5.3:+78043575	MS.gene38348:CDS
ACAACTCAGAGGGCTCATTG+GGG	0.714515	5.3:-78043727	None:intergenic
TTGCTGGATAGACAACTCAG+AGG	0.728361	5.3:-78043738	None:intergenic
AGGTGAAGAGAAGTTCACGG+AGG	0.734150	5.3:-78043439	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAAAATAAAAAGAAATACA+AGG	-	chr5.3:78043113-78043132	None:intergenic	10.0%
!!	TTAAAACATTTATTTAAGAA+CGG	-	chr5.3:78043011-78043030	None:intergenic	10.0%
!!!	ATTATATAAACTTGTTAATG+AGG	-	chr5.3:78043261-78043280	None:intergenic	15.0%
!	AACATTTATTTAAGAACGGA+TGG	-	chr5.3:78043007-78043026	None:intergenic	25.0%
!	AGCTTATATCAAAAGAAGAA+AGG	-	chr5.3:78042941-78042960	None:intergenic	25.0%
!	CTTATATCAAAAGAAGAAAG+GGG	-	chr5.3:78042939-78042958	None:intergenic	25.0%
!	GCTTATATCAAAAGAAGAAA+GGG	-	chr5.3:78042940-78042959	None:intergenic	25.0%
!	GTTTATATAATAGTTGACTG+TGG	+	chr5.3:78043270-78043289	MS.gene38348:intron	25.0%
!	TAATTGTTCTTGTATGTGTT+TGG	-	chr5.3:78042982-78043001	None:intergenic	25.0%
!	TTATATCAAAAGAAGAAAGG+GGG	-	chr5.3:78042938-78042957	None:intergenic	25.0%
	ACATATCATGAACCATTTCT+AGG	-	chr5.3:78043214-78043233	None:intergenic	30.0%
	GTTAATGAGGATTGAAATTG+AGG	-	chr5.3:78043248-78043267	None:intergenic	30.0%
	TGAAACTAGTAACCTAGAAA+TGG	+	chr5.3:78043199-78043218	MS.gene38348:intron	30.0%
!!	TATGCACTTCTTAATTTGTG+TGG	+	chr5.3:78043132-78043151	MS.gene38348:intron	30.0%
!!	TTCTTGTATGTGTTTGGTTT+TGG	-	chr5.3:78042976-78042995	None:intergenic	30.0%
	AAAATCTCAGGCTCAACAAT+AGG	-	chr5.3:78043818-78043837	None:intergenic	35.0%
	GTTGAATTAGCTGGAACAAA+TGG	+	chr5.3:78043602-78043621	MS.gene38348:CDS	35.0%
	TCTTGGAGGATTTCAACAAA+AGG	-	chr5.3:78043542-78043561	None:intergenic	35.0%
	AACTCTTCTCAAGCCTAACA+TGG	+	chr5.3:78043940-78043959	MS.gene38348:CDS	40.0%
	AATGGTGAAACAACAACCCA+AGG	+	chr5.3:78043620-78043639	MS.gene38348:CDS	40.0%
	ACCTGCATACTTTCCAACAA+AGG	-	chr5.3:78042906-78042925	None:intergenic	40.0%
	ATCATTCAGTGCCTTGTAAC+AGG	-	chr5.3:78043900-78043919	None:intergenic	40.0%
	CAAGAGAACAATGTCATACC+AGG	+	chr5.3:78043557-78043576	MS.gene38348:CDS	40.0%
	CATCAACGTTGAGAACATTG+AGG	+	chr5.3:78043403-78043422	MS.gene38348:CDS	40.0%
	CTGGTATGACATTGTTCTCT+TGG	-	chr5.3:78043559-78043578	None:intergenic	40.0%
	GGTAAAGAGTTTCCTCAAAG+AGG	-	chr5.3:78043499-78043518	None:intergenic	40.0%
	GTATGACATTGTTCTCTTGG+AGG	-	chr5.3:78043556-78043575	None:intergenic	40.0%
	TCCAAGAGAGTCAAAACCTT+GGG	-	chr5.3:78043639-78043658	None:intergenic	40.0%
	TGAGAAGAGTTCCTTCAAGA+AGG	-	chr5.3:78043931-78043950	None:intergenic	40.0%
	TTCAACAAAAGGCTTCCCAT+CGG	-	chr5.3:78043531-78043550	None:intergenic	40.0%
	TTTACCAGAATTCCTCCGAT+GGG	+	chr5.3:78043513-78043532	MS.gene38348:CDS	40.0%
!	ACCTAGTGCGAGAGTTATTT+TGG	+	chr5.3:78043160-78043179	MS.gene38348:intron	40.0%
!	GATCCATCGGTTAAAATCTC+AGG	-	chr5.3:78043830-78043849	None:intergenic	40.0%
!!	AAGGGTGTTGTTGAATTAGC+TGG	+	chr5.3:78043593-78043612	MS.gene38348:CDS	40.0%
!!	CCTAGTGCGAGAGTTATTTT+GGG	+	chr5.3:78043161-78043180	MS.gene38348:intron	40.0%
!!!	GAGAGTTATTTTGGGACAGA+GGG	+	chr5.3:78043169-78043188	MS.gene38348:intron	40.0%
	AAGCCTAACATGGTTACTCC+AGG	+	chr5.3:78043950-78043969	MS.gene38348:CDS	45.0%
	AATGCACTCCAATACCTCTC+CGG	+	chr5.3:78043467-78043486	MS.gene38348:CDS	45.0%
	ACCAGGCATCAAAGTTGACA+AGG	+	chr5.3:78043574-78043593	MS.gene38348:CDS	45.0%
	CCAGGCATCAAAGTTGACAA+GGG	+	chr5.3:78043575-78043594	MS.gene38348:CDS	45.0%
	CCCAAAATAACTCTCGCACT+AGG	-	chr5.3:78043164-78043183	None:intergenic	45.0%
	CTCCAAGAGAGTCAAAACCT+TGG	-	chr5.3:78043640-78043659	None:intergenic	45.0%
	CTTTACCAGAATTCCTCCGA+TGG	+	chr5.3:78043512-78043531	MS.gene38348:CDS	45.0%
	GAACCTGGAGTAACCATGTT+AGG	-	chr5.3:78043956-78043975	None:intergenic	45.0%
	GAGAGGTGAAGAGAAGTTCA+CGG	-	chr5.3:78043445-78043464	None:intergenic	45.0%
	GATCAGCATGTCCTTCTTGA+AGG	+	chr5.3:78043917-78043936	MS.gene38348:CDS	45.0%
	TAGATGCCAGCAGTACTACA+AGG	+	chr5.3:78043661-78043680	MS.gene38348:CDS	45.0%
	TCTATCCAGCAAAACGCACA+GGG	+	chr5.3:78043749-78043768	MS.gene38348:CDS	45.0%
	TGCTGGATAGACAACTCAGA+GGG	-	chr5.3:78043740-78043759	None:intergenic	45.0%
	TTCCTCAAAGAGGATGACAC+CGG	-	chr5.3:78043489-78043508	None:intergenic	45.0%
	TTGCTGGATAGACAACTCAG+AGG	-	chr5.3:78043741-78043760	None:intergenic	45.0%
!	GCCTTTGTTGGAAAGTATGC+AGG	+	chr5.3:78042902-78042921	MS.gene38348:CDS	45.0%
!!	GAGAGGTATTGGAGTGCATT+GGG	-	chr5.3:78043467-78043486	None:intergenic	45.0%
!!	GAGCCTGAGATTTTAACCGA+TGG	+	chr5.3:78043824-78043843	MS.gene38348:CDS	45.0%
!!!	ACCCAAGGTTTTGACTCTCT+TGG	+	chr5.3:78043635-78043654	MS.gene38348:CDS	45.0%
!!!	CGAGAGTTATTTTGGGACAG+AGG	+	chr5.3:78043168-78043187	MS.gene38348:intron	45.0%
	AACAAAAGGCTTCCCATCGG+AGG	-	chr5.3:78043528-78043547	None:intergenic	50.0%
	ACAACTCAGAGGGCTCATTG+GGG	-	chr5.3:78043730-78043749	None:intergenic	50.0%
	AGACAACTCAGAGGGCTCAT+TGG	-	chr5.3:78043732-78043751	None:intergenic	50.0%
	AGGTGAAGAGAAGTTCACGG+AGG	-	chr5.3:78043442-78043461	None:intergenic	50.0%
	CAGGCTCAACAATAGGCACA+AGG	-	chr5.3:78043811-78043830	None:intergenic	50.0%
	CCCTTGTCAACTTTGATGCC+TGG	-	chr5.3:78043578-78043597	None:intergenic	50.0%
	GACAACTCAGAGGGCTCATT+GGG	-	chr5.3:78043731-78043750	None:intergenic	50.0%
	GCCATCATTTGCCAGGAGAA+TGG	+	chr5.3:78043785-78043804	MS.gene38348:CDS	50.0%
	GCCATTCTCCTGGCAAATGA+TGG	-	chr5.3:78043789-78043808	None:intergenic	50.0%
	GGCGATGTCATGAGATCCAT+CGG	-	chr5.3:78043843-78043862	None:intergenic	50.0%
	GGTGAAGAGAAGTTCACGGA+GGG	-	chr5.3:78043441-78043460	None:intergenic	50.0%
	GTATTGGAGTGCATTGGGAG+AGG	-	chr5.3:78043462-78043481	None:intergenic	50.0%
	GTCTATCCAGCAAAACGCAC+AGG	+	chr5.3:78043748-78043767	MS.gene38348:CDS	50.0%
	TGTGCTTGCAGCCTGTTACA+AGG	+	chr5.3:78043886-78043905	MS.gene38348:CDS	50.0%
	TTCAGTGACAGCAGCGCATT+TGG	-	chr5.3:78043864-78043883	None:intergenic	50.0%
!!	GATGAGAGCACTGGAACCAT+TGG	+	chr5.3:78043365-78043384	MS.gene38348:CDS	50.0%
!!	GGAGAGGTATTGGAGTGCAT+TGG	-	chr5.3:78043468-78043487	None:intergenic	50.0%
	AGTACTACAAGGCCGGAGCA+CGG	+	chr5.3:78043672-78043691	MS.gene38348:CDS	55.0%
	CAAAGAGGATGACACCGGAG+AGG	-	chr5.3:78043484-78043503	None:intergenic	55.0%
	CTGCCAAGATACCCTTGCCA+GGG	-	chr5.3:78043343-78043362	None:intergenic	55.0%
	GAAGTTCACGGAGGGCTTGA+CGG	-	chr5.3:78043433-78043452	None:intergenic	55.0%
	GCTTCCCATCGGAGGAATTC+TGG	-	chr5.3:78043520-78043539	None:intergenic	55.0%
	TACATAGCCACCCCTGGCAA+GGG	+	chr5.3:78043329-78043348	MS.gene38348:CDS	55.0%
	TAGGCACAAGGCCATTCTCC+TGG	-	chr5.3:78043799-78043818	None:intergenic	55.0%
	TCGCTACGCCATCATTTGCC+AGG	+	chr5.3:78043778-78043797	MS.gene38348:CDS	55.0%
	TGCCAAGATACCCTTGCCAG+GGG	-	chr5.3:78043342-78043361	None:intergenic	55.0%
	TGCCAGCAGTACTACAAGGC+CGG	+	chr5.3:78043665-78043684	MS.gene38348:CDS	55.0%
	TGGCGTGCGGTACTCAAGAT+TGG	+	chr5.3:78043704-78043723	MS.gene38348:CDS	55.0%
!	GCTTGCTAGACGCTTGCCAA+TGG	-	chr5.3:78043384-78043403	None:intergenic	55.0%
!	GGATGACACCGGAGAGGTAT+TGG	-	chr5.3:78043478-78043497	None:intergenic	55.0%
!	TTGGCAGCAGATGAGAGCAC+TGG	+	chr5.3:78043356-78043375	MS.gene38348:CDS	55.0%
!!	CTCCGGTGTCATCCTCTTTG+AGG	+	chr5.3:78043484-78043503	MS.gene38348:CDS	55.0%
	ACGGTTTGCCAAGTGGCGTG+CGG	+	chr5.3:78043691-78043710	MS.gene38348:CDS	60.0%
	CAAGATACCCTTGCCAGGGG+TGG	-	chr5.3:78043339-78043358	None:intergenic	60.0%
	CCACTTGGCAAACCGTGCTC+CGG	-	chr5.3:78043687-78043706	None:intergenic	60.0%
	CCAGCAAAACGCACAGGGCT+TGG	+	chr5.3:78043754-78043773	MS.gene38348:CDS	60.0%
	GCCAAGTACATAGCCACCCC+TGG	+	chr5.3:78043323-78043342	MS.gene38348:CDS	60.0%
	GCCAGGGGTGGCTATGTACT+TGG	-	chr5.3:78043327-78043346	None:intergenic	60.0%
	GCTGCCAAGATACCCTTGCC+AGG	-	chr5.3:78043344-78043363	None:intergenic	60.0%
	GTACATAGCCACCCCTGGCA+AGG	+	chr5.3:78043328-78043347	MS.gene38348:CDS	60.0%
	TTCACGGAGGGCTTGACGGT+TGG	-	chr5.3:78043429-78043448	None:intergenic	60.0%
!	CCAAGCCCTGTGCGTTTTGC+TGG	-	chr5.3:78043757-78043776	None:intergenic	60.0%
!	CTCCGGCCTTGTAGTACTGC+TGG	-	chr5.3:78043670-78043689	None:intergenic	60.0%
!	CTTGAGTACCGCACGCCACT+TGG	-	chr5.3:78043702-78043721	None:intergenic	60.0%
!!	CACCCCTGGCAAGGGTATCT+TGG	+	chr5.3:78043337-78043356	MS.gene38348:CDS	60.0%
	CCGGAGCACGGTTTGCCAAG+TGG	+	chr5.3:78043684-78043703	MS.gene38348:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	78042896	78043988	78042896	ID=MS.gene38348
chr5.3	mRNA	78042896	78043988	78042896	ID=MS.gene38348.t1;Parent=MS.gene38348
chr5.3	exon	78042896	78042923	78042896	ID=MS.gene38348.t1.exon1;Parent=MS.gene38348.t1
chr5.3	CDS	78042896	78042923	78042896	ID=cds.MS.gene38348.t1;Parent=MS.gene38348.t1
chr5.3	exon	78043306	78043988	78043306	ID=MS.gene38348.t1.exon2;Parent=MS.gene38348.t1
chr5.3	CDS	78043306	78043988	78043306	ID=cds.MS.gene38348.t1;Parent=MS.gene38348.t1

Gene Sequence

>MS.gene38348

ATGTCGGCCTTTGTTGGAAAGTATGCAGGTAACTTCATTCCCCCTTTCTTCTTTTGATATAAGCTTACTCACTCTGACCAAAACCAAACACATACAAGAACAATTACTCCATCCGTTCTTAAATAAATGTTTTAATATATAAAAAATGTATTAAAATAAGTGTCGTTTTGCTTTTTTTTGTATGACATTAATTTTTTTTTTCTTACAAATTTTACCTTGTATTTCTTTTTATTTTATATGCACTTCTTAATTTGTGTGGAAAAAACCTAGTGCGAGAGTTATTTTGGGACAGAGGGAGTAACTTGAAACTAGTAACCTAGAAATGGTTCATGATATGTCTAAGAAGCTACCTCAATTTCAATCCTCATTAACAAGTTTATATAATAGTTGACTGTGGTTTTCATTTCAAGATGAGCTTATCAAGAATGCCAAGTACATAGCCACCCCTGGCAAGGGTATCTTGGCAGCAGATGAGAGCACTGGAACCATTGGCAAGCGTCTAGCAAGCATCAACGTTGAGAACATTGAGGCCAACCGTCAAGCCCTCCGTGAACTTCTCTTCACCTCTCCCAATGCACTCCAATACCTCTCCGGTGTCATCCTCTTTGAGGAAACTCTTTACCAGAATTCCTCCGATGGGAAGCCTTTTGTTGAAATCCTCCAAGAGAACAATGTCATACCAGGCATCAAAGTTGACAAGGGTGTTGTTGAATTAGCTGGAACAAATGGTGAAACAACAACCCAAGGTTTTGACTCTCTTGGAGCTAGATGCCAGCAGTACTACAAGGCCGGAGCACGGTTTGCCAAGTGGCGTGCGGTACTCAAGATTGGCCCCAATGAGCCCTCTGAGTTGTCTATCCAGCAAAACGCACAGGGCTTGGCTCGCTACGCCATCATTTGCCAGGAGAATGGCCTTGTGCCTATTGTTGAGCCTGAGATTTTAACCGATGGATCTCATGACATCGCCAAATGCGCTGCTGTCACTGAAACTGTGCTTGCAGCCTGTTACAAGGCACTGAATGATCAGCATGTCCTTCTTGAAGGAACTCTTCTCAAGCCTAACATGGTTACTCCAGGTTCTGATAGCCCAAAG

Protein sequence

>MS.gene38348.t1

MSAFVGKYADELIKNAKYIATPGKGILAADESTGTIGKRLASINVENIEANRQALRELLFTSPNALQYLSGVILFEETLYQNSSDGKPFVEILQENNVIPGIKVDKGVVELAGTNGETTTQGFDSLGARCQQYYKAGARFAKWRAVLKIGPNEPSELSIQQNAQGLARYAIICQENGLVPIVEPEILTDGSHDIAKCAAVTETVLAACYKALNDQHVLLEGTLLKPNMVTPGSDSPK