AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006759.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006759.01.T01 MTR_2g014360 98.684 152 2 0 1 152 1 152 3.05e-109 307
MsG0280006759.01.T01 MTR_8g064590 68.841 138 37 2 14 151 5 136 3.69e-65 195
MsG0280006759.01.T01 MTR_2g090635 66.667 144 40 2 12 152 2 140 4.38e-65 195
MsG0280006759.01.T01 MTR_5g034290 65.217 138 42 2 13 150 4 135 5.17e-60 182
MsG0280006759.01.T01 MTR_5g034290 65.217 138 42 2 13 150 37 168 1.38e-59 182
MsG0280006759.01.T01 MTR_6g060020 60.000 105 35 1 47 151 2 99 2.93e-38 126
MsG0280006759.01.T01 MTR_5g083900 38.760 129 64 2 20 147 111 225 1.43e-20 84.3
MsG0280006759.01.T01 MTR_5g083870 39.259 135 63 4 20 149 5 125 1.56e-20 81.6
MsG0280006759.01.T01 MTR_2g090640 54.321 81 33 1 69 145 456 536 5.91e-18 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006759.01.T01 AT2G33845 68.382 136 38 1 17 152 52 182 1.95e-64 195
MsG0280006759.01.T01 AT1G23750 63.121 141 46 1 11 151 3 137 6.08e-63 189
MsG0280006759.01.T01 AT4G28440 65.152 132 46 0 17 148 17 148 2.55e-59 181
MsG0280006759.01.T01 AT1G10590 61.940 134 46 3 16 148 7 136 2.21e-56 173
MsG0280006759.01.T01 AT1G10590 61.940 134 46 3 16 148 7 136 2.21e-56 173
MsG0280006759.01.T01 AT1G10590 61.940 134 46 3 16 148 21 150 3.61e-56 173
MsG0280006759.01.T01 AT1G03810 60.902 133 49 1 17 149 14 143 3.20e-54 168
MsG0280006759.01.T01 AT1G23750 68.571 105 27 1 47 151 2 100 1.34e-47 149

Find 35 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAAGATTGACATGTTTA+AGG 0.167888 2:+5927094 MsG0280006759.01.T01:CDS
TCCGAAACTGTTCTGCAAAA+AGG 0.301507 2:+5925823 MsG0280006759.01.T01:CDS
ATGAAGTCAAGCATGTTAAA+AGG 0.324760 2:-5927342 None:intergenic
AAAGTGTGTCCGTTTGTTCC+TGG 0.370124 2:-5925781 None:intergenic
GCAAAGATTGACATGTTTAA+GGG 0.371017 2:+5927095 MsG0280006759.01.T01:CDS
ATCTCTCATTGAATATGAAT+TGG 0.375517 2:+5927202 MsG0280006759.01.T01:CDS
ATTCATATTCAATGAGAGAT+AGG 0.377306 2:-5927199 None:intergenic
TGAGGCTGGCCGTTGACAAA+TGG 0.402663 2:+5927123 MsG0280006759.01.T01:CDS
GAGGCTGGCCGTTGACAAAT+GGG 0.404101 2:+5927124 MsG0280006759.01.T01:CDS
CTGATATGGCAAAACTTAAC+TGG 0.406337 2:+5927285 MsG0280006759.01.T01:three_prime_UTR
TGCAGAGTTCGTTGTTAAAG+AGG 0.437457 2:+5927169 MsG0280006759.01.T01:CDS
AATGTTGTTTCACATGAAGA+TGG 0.450601 2:+5925706 MsG0280006759.01.T01:CDS
AAAACCCACTTTCACAAAAG+TGG 0.453220 2:+5925747 MsG0280006759.01.T01:CDS
CATTGCAGTTGAGCTTATGA+AGG 0.477921 2:+5927046 MsG0280006759.01.T01:intron
AGAGTAATTCATAGTTAAAG+AGG 0.491467 2:+5927238 MsG0280006759.01.T01:exon
TCTTTAACAACGAACTCTGC+AGG 0.492103 2:-5927167 None:intergenic
GCAGTTGAGCTTATGAAGGC+TGG 0.493996 2:+5927050 MsG0280006759.01.T01:intron
TGTCTCATCGGTGATGAAAC+TGG 0.501588 2:+5925907 MsG0280006759.01.T01:CDS
TATCAGCACAAACAAAAGAT+AGG 0.506308 2:-5927268 None:intergenic
CAGATGAAACCAGGAACAAA+CGG 0.508711 2:+5925772 MsG0280006759.01.T01:CDS
CGGACACACTTTGATTGCTA+AGG 0.513860 2:+5925792 MsG0280006759.01.T01:CDS
CTTATCTCTGAGTGTCTCAT+CGG 0.563970 2:+5925895 MsG0280006759.01.T01:CDS
TTCACCGCTCGCAATGAACA+AGG 0.567422 2:+5925940 MsG0280006759.01.T01:CDS
ATTGCGAGCGGTGAAAATGA+TGG 0.574482 2:-5925932 None:intergenic
GTTTAAGGGATCAATGAGGC+TGG 0.582292 2:+5927109 MsG0280006759.01.T01:CDS
GGCTGGCCGTTGACAAATGG+GGG 0.597410 2:+5927126 MsG0280006759.01.T01:CDS
AGACACTCAGAGATAAGAGT+AGG 0.601286 2:-5925889 None:intergenic
CTTTAACAACGAACTCTGCA+GGG 0.601895 2:-5927166 None:intergenic
GAGTAATTCATAGTTAAAGA+GGG 0.610715 2:+5927239 MsG0280006759.01.T01:exon
ATACCACGAGAAGATGAAGA+AGG 0.617287 2:-5925859 None:intergenic
AGAACCTTGTTCATTGCGAG+CGG 0.622173 2:-5925944 None:intergenic
AGACCTTCTTCATCTTCTCG+TGG 0.632890 2:+5925856 MsG0280006759.01.T01:CDS
AGGCTGGCCGTTGACAAATG+GGG 0.640279 2:+5927125 MsG0280006759.01.T01:CDS
AAAGTGGATCAGATGAAACC+AGG 0.663122 2:+5925763 MsG0280006759.01.T01:CDS
ACATGTTTAAGGGATCAATG+AGG 0.703797 2:+5927105 MsG0280006759.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTTCTTCTTCATTT+GGG + Chr2:5926055-5926074 MsG0280006759.01.T01:intron 15.0%
!!! TAAGATATGTTTTTATTGAT+TGG + Chr2:5926963-5926982 MsG0280006759.01.T01:intron 15.0%
!! AATCTAAATTAGTGTTCATA+AGG + Chr2:5926133-5926152 MsG0280006759.01.T01:intron 20.0%
!! ATAAAAATGCTGATAAAAAG+GGG - Chr2:5926256-5926275 None:intergenic 20.0%
!! CATAAAAATGCTGATAAAAA+GGG - Chr2:5926257-5926276 None:intergenic 20.0%
!! TTATGAAACTTATGATTCAT+TGG + Chr2:5926411-5926430 MsG0280006759.01.T01:intron 20.0%
!!! ACAGTTATCATTTTTAAGAA+TGG - Chr2:5926001-5926020 None:intergenic 20.0%
!!! ATGAACACTAATTTAGATTT+TGG - Chr2:5926132-5926151 None:intergenic 20.0%
!!! ATTTTATTAAGAGTTTAGAG+AGG + Chr2:5926362-5926381 MsG0280006759.01.T01:intron 20.0%
!!! CAGTTATCATTTTTAAGAAT+GGG - Chr2:5926000-5926019 None:intergenic 20.0%
!!! GATTTTTTTTCTTCTTCATT+TGG + Chr2:5926054-5926073 MsG0280006759.01.T01:intron 20.0%
!!! GTTTTTATTGATTGGATATT+AGG + Chr2:5926971-5926990 MsG0280006759.01.T01:intron 20.0%
!!! TTTTTTTTCTTCTTCATTTG+GGG + Chr2:5926056-5926075 MsG0280006759.01.T01:intron 20.0%
! AATAAGTTGTTTATCCAAGT+AGG + Chr2:5926805-5926824 MsG0280006759.01.T01:intron 25.0%
! AGAGTAATTCATAGTTAAAG+AGG + Chr2:5927238-5927257 MsG0280006759.01.T01:exon 25.0%
! ATCTCTCATTGAATATGAAT+TGG + Chr2:5927202-5927221 MsG0280006759.01.T01:CDS 25.0%
! ATTCATATTCAATGAGAGAT+AGG - Chr2:5927202-5927221 None:intergenic 25.0%
! CAATATAGAAAGAAATAGTC+AGG - Chr2:5926342-5926361 None:intergenic 25.0%
! CTTATTTCCATAACATCTAT+AGG + Chr2:5926666-5926685 MsG0280006759.01.T01:intron 25.0%
! GAGTAATTCATAGTTAAAGA+GGG + Chr2:5927239-5927258 MsG0280006759.01.T01:exon 25.0%
! GCATAAAAATGCTGATAAAA+AGG - Chr2:5926258-5926277 None:intergenic 25.0%
! TATTAGGACTAATCAGTATA+AGG + Chr2:5926987-5927006 MsG0280006759.01.T01:intron 25.0%
! TCTACAACATGGTAATATAA+AGG - Chr2:5926734-5926753 None:intergenic 25.0%
! TTATAGTATAACCATGTAAG+TGG - Chr2:5926778-5926797 None:intergenic 25.0%
!! AAACTTATGATTCATTGGTT+GGG + Chr2:5926416-5926435 MsG0280006759.01.T01:intron 25.0%
!! ACATCAATAGTTTCTTTTAG+TGG + Chr2:5926932-5926951 MsG0280006759.01.T01:intron 25.0%
!! ACTATCATATTTTTCTCAAG+TGG - Chr2:5927434-5927453 None:intergenic 25.0%
!! CATTTTCACTTTAATTAGAC+TGG + Chr2:5927314-5927333 MsG0280006759.01.T01:three_prime_UTR 25.0%
!!! AATAAGCTATTTCTACAACA+TGG - Chr2:5926745-5926764 None:intergenic 25.0%
!!! ATTTTTAGCTCTTTATGTAC+TGG + Chr2:5926307-5926326 MsG0280006759.01.T01:intron 25.0%
!!! GATCTACATTTTTTGAGATT+AGG + Chr2:5926091-5926110 MsG0280006759.01.T01:intron 25.0%
!!! TTGTAGTTTATTTTCTTCGT+GGG + Chr2:5926829-5926848 MsG0280006759.01.T01:intron 25.0%
AAAAATGATAACTGTTCTCG+TGG + Chr2:5926006-5926025 MsG0280006759.01.T01:intron 30.0%
AAAATGATAACTGTTCTCGT+GGG + Chr2:5926007-5926026 MsG0280006759.01.T01:intron 30.0%
AAACACTTGTGACACTATTT+GGG + Chr2:5926597-5926616 MsG0280006759.01.T01:intron 30.0%
AAAGAAACTATTGATGTTGC+AGG - Chr2:5926929-5926948 None:intergenic 30.0%
AAATAAACTACAAGCCTACT+TGG - Chr2:5926822-5926841 None:intergenic 30.0%
AAGAAACTATTGATGTTGCA+GGG - Chr2:5926928-5926947 None:intergenic 30.0%
AAGCTATCCTATAGATGTTA+TGG - Chr2:5926676-5926695 None:intergenic 30.0%
ATGAAGTCAAGCATGTTAAA+AGG - Chr2:5927345-5927364 None:intergenic 30.0%
GAAACTTATGATTCATTGGT+TGG + Chr2:5926415-5926434 MsG0280006759.01.T01:intron 30.0%
GCAAAGATTGACATGTTTAA+GGG + Chr2:5927095-5927114 MsG0280006759.01.T01:CDS 30.0%
GGGGATCTAAAAATAAACAT+TGG - Chr2:5926237-5926256 None:intergenic 30.0%
TAAACACTTGTGACACTATT+TGG + Chr2:5926596-5926615 MsG0280006759.01.T01:intron 30.0%
TATCAGCACAAACAAAAGAT+AGG - Chr2:5927271-5927290 None:intergenic 30.0%
TATTAAGAGTTTAGAGAGGA+AGG + Chr2:5926366-5926385 MsG0280006759.01.T01:intron 30.0%
TGCAAAGATTGACATGTTTA+AGG + Chr2:5927094-5927113 MsG0280006759.01.T01:CDS 30.0%
! AACATGCTTGACTTCATTTT+AGG + Chr2:5927348-5927367 MsG0280006759.01.T01:three_prime_UTR 30.0%
!! AATGTTGTTTCACATGAAGA+TGG + Chr2:5925706-5925725 MsG0280006759.01.T01:CDS 30.0%
!! ATCTTTTGTTTGTGCTGATA+TGG + Chr2:5927271-5927290 MsG0280006759.01.T01:three_prime_UTR 30.0%
!! CTTGTAGTTTATTTTCTTCG+TGG + Chr2:5926828-5926847 MsG0280006759.01.T01:intron 30.0%
!! TGTAGTTTATTTTCTTCGTG+GGG + Chr2:5926830-5926849 MsG0280006759.01.T01:intron 30.0%
AAAACCCACTTTCACAAAAG+TGG + Chr2:5925747-5925766 MsG0280006759.01.T01:CDS 35.0%
ACATGTTTAAGGGATCAATG+AGG + Chr2:5927105-5927124 MsG0280006759.01.T01:CDS 35.0%
AGCAAATCCTTGAGTAAGTA+GGG + Chr2:5926536-5926555 MsG0280006759.01.T01:intron 35.0%
AGTTTAGAGAGGAAGGAAAA+TGG + Chr2:5926475-5926494 MsG0280006759.01.T01:intron 35.0%
CACAAGCATTTGCATATAAG+TGG + Chr2:5926868-5926887 MsG0280006759.01.T01:intron 35.0%
CATTCTCAATCATTCCTTCA+GGG + Chr2:5926513-5926532 MsG0280006759.01.T01:intron 35.0%
CTGATATGGCAAAACTTAAC+TGG + Chr2:5927285-5927304 MsG0280006759.01.T01:three_prime_UTR 35.0%
GTGTTCATAAGGTTCTTAGT+TGG + Chr2:5926144-5926163 MsG0280006759.01.T01:intron 35.0%
GTTTAGAGAGGAAGGAAAAT+GGG + Chr2:5926476-5926495 MsG0280006759.01.T01:intron 35.0%
TATGCAAATGCTTGTGCATA+AGG - Chr2:5926864-5926883 None:intergenic 35.0%
TCATTCTCAATCATTCCTTC+AGG + Chr2:5926512-5926531 MsG0280006759.01.T01:intron 35.0%
! ACCTTTTTGCAGAACAGTTT+CGG - Chr2:5925827-5925846 None:intergenic 35.0%
!! TGATCCACTTTTGTGAAAGT+GGG - Chr2:5925754-5925773 None:intergenic 35.0%
AAAGTGGATCAGATGAAACC+AGG + Chr2:5925763-5925782 MsG0280006759.01.T01:CDS 40.0%
AGACACTCAGAGATAAGAGT+AGG - Chr2:5925892-5925911 None:intergenic 40.0%
ATACCACGAGAAGATGAAGA+AGG - Chr2:5925862-5925881 None:intergenic 40.0%
CAAACAACCCTACTTACTCA+AGG - Chr2:5926546-5926565 None:intergenic 40.0%
CAGATGAAACCAGGAACAAA+CGG + Chr2:5925772-5925791 MsG0280006759.01.T01:CDS 40.0%
CATGTCTCATGAGTTTAGAG+AGG + Chr2:5926464-5926483 MsG0280006759.01.T01:intron 40.0%
CATTGCAGTTGAGCTTATGA+AGG + Chr2:5927046-5927065 MsG0280006759.01.T01:intron 40.0%
CCGAGTAACAACACCAAAAT+GGG - Chr2:5926181-5926200 None:intergenic 40.0%
CTCTCTAAACTCATGAGACA+TGG - Chr2:5926466-5926485 None:intergenic 40.0%
CTTATCTCTGAGTGTCTCAT+CGG + Chr2:5925895-5925914 MsG0280006759.01.T01:CDS 40.0%
CTTGAGTAAGTAGGGTTGTT+TGG + Chr2:5926544-5926563 MsG0280006759.01.T01:intron 40.0%
CTTTAACAACGAACTCTGCA+GGG - Chr2:5927169-5927188 None:intergenic 40.0%
GAAACTGTTCTGCAAAAAGG+TGG + Chr2:5925826-5925845 MsG0280006759.01.T01:CDS 40.0%
GAGCAAATCCTTGAGTAAGT+AGG + Chr2:5926535-5926554 MsG0280006759.01.T01:intron 40.0%
GTAAGTAGGGTTGTTTGGAT+TGG + Chr2:5926549-5926568 MsG0280006759.01.T01:intron 40.0%
TCCGAAACTGTTCTGCAAAA+AGG + Chr2:5925823-5925842 MsG0280006759.01.T01:CDS 40.0%
TCCGAGTAACAACACCAAAA+TGG - Chr2:5926182-5926201 None:intergenic 40.0%
TCTCATGAGTTTAGAGAGGA+AGG + Chr2:5926468-5926487 MsG0280006759.01.T01:intron 40.0%
TCTTTAACAACGAACTCTGC+AGG - Chr2:5927170-5927189 None:intergenic 40.0%
TGCAGAGTTCGTTGTTAAAG+AGG + Chr2:5927169-5927188 MsG0280006759.01.T01:CDS 40.0%
! ATTCATTGGTTGGGCTTTAG+GGG + Chr2:5926425-5926444 MsG0280006759.01.T01:intron 40.0%
! GATTCATTGGTTGGGCTTTA+GGG + Chr2:5926424-5926443 MsG0280006759.01.T01:intron 40.0%
! GGTGATTGTTACACCCATTT+TGG + Chr2:5926165-5926184 MsG0280006759.01.T01:intron 40.0%
! TGATTCATTGGTTGGGCTTT+AGG + Chr2:5926423-5926442 MsG0280006759.01.T01:intron 40.0%
! TTTTTGCAGAACAGTTTCGG+AGG - Chr2:5925824-5925843 None:intergenic 40.0%
!! AAAGTGGGTTTTCTCTTTGC+GGG - Chr2:5925739-5925758 None:intergenic 40.0%
!! CTGATCCACTTTTGTGAAAG+TGG - Chr2:5925755-5925774 None:intergenic 40.0%
!! GAAAGTGGGTTTTCTCTTTG+CGG - Chr2:5925740-5925759 None:intergenic 40.0%
!! TGGGCTTTAGGGGATAAAAA+TGG + Chr2:5926435-5926454 MsG0280006759.01.T01:intron 40.0%
!!! CCCATTTTGGTGTTGTTACT+CGG + Chr2:5926178-5926197 MsG0280006759.01.T01:intron 40.0%
AAAGTGTGTCCGTTTGTTCC+TGG - Chr2:5925784-5925803 None:intergenic 45.0%
ACTGTTCTGCAAAAAGGTGG+AGG + Chr2:5925829-5925848 MsG0280006759.01.T01:CDS 45.0%
AGAACCTTGTTCATTGCGAG+CGG - Chr2:5925947-5925966 None:intergenic 45.0%
AGACCTTCTTCATCTTCTCG+TGG + Chr2:5925856-5925875 MsG0280006759.01.T01:CDS 45.0%
AGAGGAAGGAAAATGGGAGT+AGG + Chr2:5926482-5926501 MsG0280006759.01.T01:intron 45.0%
ATTGCGAGCGGTGAAAATGA+TGG - Chr2:5925935-5925954 None:intergenic 45.0%
CGGACACACTTTGATTGCTA+AGG + Chr2:5925792-5925811 MsG0280006759.01.T01:CDS 45.0%
GTTTAAGGGATCAATGAGGC+TGG + Chr2:5927109-5927128 MsG0280006759.01.T01:CDS 45.0%
TCGCAGTCGATTGCAAATTG+TGG - Chr2:5926903-5926922 None:intergenic 45.0%
TGTCTCATCGGTGATGAAAC+TGG + Chr2:5925907-5925926 MsG0280006759.01.T01:CDS 45.0%
AATGCGCCCCCATTTGTCAA+CGG - Chr2:5927135-5927154 None:intergenic 50.0%
GCAGTTGAGCTTATGAAGGC+TGG + Chr2:5927050-5927069 MsG0280006759.01.T01:intron 50.0%
TCTTAAGCGCGCCACTTACA+TGG + Chr2:5926764-5926783 MsG0280006759.01.T01:intron 50.0%
TTCACCGCTCGCAATGAACA+AGG + Chr2:5925940-5925959 MsG0280006759.01.T01:CDS 50.0%
! CTCAAGGATTTGCTCCCTGA+AGG - Chr2:5926530-5926549 None:intergenic 50.0%
! GGGTTTTCTCTTTGCGGGTT+TGG - Chr2:5925734-5925753 None:intergenic 50.0%
! AGGCTGGCCGTTGACAAATG+GGG + Chr2:5927125-5927144 MsG0280006759.01.T01:CDS 55.0%
! GAGGCTGGCCGTTGACAAAT+GGG + Chr2:5927124-5927143 MsG0280006759.01.T01:CDS 55.0%
! TGACAAATGGGGGCGCATTG+AGG + Chr2:5927136-5927155 MsG0280006759.01.T01:CDS 55.0%
! TGAGGCTGGCCGTTGACAAA+TGG + Chr2:5927123-5927142 MsG0280006759.01.T01:CDS 55.0%
! GGCTGGCCGTTGACAAATGG+GGG + Chr2:5927126-5927145 MsG0280006759.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 5925694 5927455 5925694 ID=MsG0280006759.01;Name=MsG0280006759.01
Chr2 mRNA 5925694 5927455 5925694 ID=MsG0280006759.01.T01;Parent=MsG0280006759.01;Name=MsG0280006759.01.T01;_AED=0.33;_eAED=0.33;_QI=0|1|0.5|1|1|1|2|211|152
Chr2 exon 5925694 5925961 5925694 ID=MsG0280006759.01.T01:exon:2387;Parent=MsG0280006759.01.T01
Chr2 exon 5927054 5927455 5927054 ID=MsG0280006759.01.T01:exon:2388;Parent=MsG0280006759.01.T01
Chr2 CDS 5925694 5925961 5925694 ID=MsG0280006759.01.T01:cds;Parent=MsG0280006759.01.T01
Chr2 CDS 5927054 5927244 5927054 ID=MsG0280006759.01.T01:cds;Parent=MsG0280006759.01.T01
Chr2 three_prime_UTR 5927245 5927455 5927245 ID=MsG0280006759.01.T01:three_prime_utr;Parent=MsG0280006759.01.T01
Gene Sequence

>MsG0280006759.01.T01

ATGGCATCATCGAATGTTGTTTCACATGAAGATGGAACCAAACCCGCAAAGAGAAAACCCACTTTCACAAAAGTGGATCAGATGAAACCAGGAACAAACGGACACACTTTGATTGCTAAGGTTTTAACCTCCGAAACTGTTCTGCAAAAAGGTGGAGGAACAAGACCTTCTTCATCTTCTCGTGGTATTGTTCGTCCTACTCTTATCTCTGAGTGTCTCATCGGTGATGAAACTGGTACCATCATTTTCACCGCTCGCAATGAACAAGTTGAGCTTATGAAGGCTGGAAATACAGTAATCATTCGCAATGCAAAGATTGACATGTTTAAGGGATCAATGAGGCTGGCCGTTGACAAATGGGGGCGCATTGAGGTCACTGACCCTGCAGAGTTCGTTGTTAAAGAGGATAACAACCTATCTCTCATTGAATATGAATTGGTGAACGCAGTTGAAGAGTAA

Protein sequence

>MsG0280006759.01.T01

MASSNVVSHEDGTKPAKRKPTFTKVDQMKPGTNGHTLIAKVLTSETVLQKGGGTRPSSSSRGIVRPTLISECLIGDETGTIIFTARNEQVELMKAGNTVIIRNAKIDMFKGSMRLAVDKWGRIEVTDPAEFVVKEDNNLSLIEYELVNAVEE*