AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25677


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25677.t1 MTR_7g107680 86.813 182 19 2 1 177 1 182 2.76e-100 286
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25677.t1 AT4G03150 60.000 130 50 1 44 171 52 181 5.78e-52 164

Find 0 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACTTTCCTTATAAGAAA+TGG + 51704:4748-4767 MS.gene25677:intergenic 20.0%
!!! TTTTTCCATTCACAATTATT+AGG - 51704:4551-4570 MS.gene25677:intron 20.0%
! ATTGGAAACCATTTCTTATA+AGG - 51704:4737-4756 MS.gene25677:CDS 25.0%
! GAATTGGTTACATATTGTTA+AGG - 51704:4313-4332 MS.gene25677:intron 25.0%
! TGATTCCTAATAATTGTGAA+TGG + 51704:4559-4578 MS.gene25677:intergenic 25.0%
!! CTTATAAGGAAAGTTTTCAT+GGG - 51704:4751-4770 MS.gene25677:CDS 25.0%
!! TCTTATAAGGAAAGTTTTCA+TGG - 51704:4750-4769 MS.gene25677:CDS 25.0%
!! TTAGGGTTACTTTACATAAA+TGG - 51704:4586-4605 MS.gene25677:CDS 25.0%
!! TTATAAGGAAAGTTTTCATG+GGG - 51704:4752-4771 MS.gene25677:CDS 25.0%
AAATGGATGAGAATGTGAAT+GGG + 51704:4142-4161 MS.gene25677:intergenic 30.0%
ACAATTATTAGGAATCAGAG+TGG - 51704:4562-4581 MS.gene25677:intron 30.0%
CAAAGAATTGTAGTAGGATT+TGG + 51704:4667-4686 MS.gene25677:intergenic 30.0%
CCTACTACAATTCTTTGATA+CGG - 51704:4670-4689 MS.gene25677:CDS 30.0%
TGAGATGTTCATGTTAGAAT+TGG - 51704:4297-4316 MS.gene25677:intron 30.0%
! AGCTGACATTTTGTTGAATA+TGG - 51704:4707-4726 MS.gene25677:CDS 30.0%
! GGTTACTTTACATAAATGGT+TGG - 51704:4590-4609 MS.gene25677:CDS 30.0%
!! ACGTGTTTCTAAAATTTGAG+AGG + 51704:4262-4281 MS.gene25677:intergenic 30.0%
ATGAAGATATAAATGAGCGC+AGG + 51704:4521-4540 MS.gene25677:intergenic 35.0%
CCGTATCAAAGAATTGTAGT+AGG + 51704:4673-4692 MS.gene25677:intergenic 35.0%
GAAATGGATGAGAATGTGAA+TGG + 51704:4143-4162 MS.gene25677:intergenic 35.0%
! AAGATGAAGAAGAAGCGTAT+TGG + 51704:4086-4105 MS.gene25677:intergenic 35.0%
!! AATGAAAAGAGTACCTACCT+GGG + 51704:4235-4254 MS.gene25677:intergenic 35.0%
AAAGTAATCACATGTGTCCG+TGG - 51704:4442-4461 MS.gene25677:intron 40.0%
ATCTCCACATTTGTTTCCTC+AGG + 51704:4630-4649 MS.gene25677:intergenic 40.0%
ATTAGGAATCAGAGTGGCTT+AGG - 51704:4568-4587 MS.gene25677:intron 40.0%
CTAGTGATTTCAACACCGAA+AGG + 51704:4468-4487 MS.gene25677:intergenic 40.0%
GTGAGTGAATGTTACTGTCA+CGG + 51704:4061-4080 MS.gene25677:intergenic 40.0%
GTTGAAATCACTAGGTGGTT+AGG - 51704:4474-4493 MS.gene25677:intron 40.0%
GTTGAATATGGCACGTGAAT+TGG - 51704:4719-4738 MS.gene25677:CDS 40.0%
TTAGGAATCAGAGTGGCTTA+GGG - 51704:4569-4588 MS.gene25677:intron 40.0%
! ATGAAGAAGAAGCGTATTGG+TGG + 51704:4083-4102 MS.gene25677:intergenic 40.0%
! CGAGTAGTGTGGACAATTTT+TGG + 51704:4117-4136 MS.gene25677:intergenic 40.0%
!! CTTTCGGTGTTGAAATCACT+AGG - 51704:4466-4485 MS.gene25677:intron 40.0%
!! GAATGAAAAGAGTACCTACC+TGG + 51704:4236-4255 MS.gene25677:intergenic 40.0%
!!! GTGGACAATTTTTGGTGAAG+AGG + 51704:4109-4128 MS.gene25677:intergenic 40.0%
AGGAGGAAGATGGTGTGAAT+GGG + 51704:4038-4057 MS.gene25677:intergenic 45.0%
AGTGAATGTTACTGTCACGG+AGG + 51704:4058-4077 MS.gene25677:intergenic 45.0%
ATTTCAACACCGAAAGGCCA+CGG + 51704:4462-4481 MS.gene25677:intergenic 45.0%
CTGTCCTGAGGAAACAAATG+TGG - 51704:4623-4642 MS.gene25677:CDS 45.0%
GAACACGAAGGTCTTCAACT+TGG + 51704:4182-4201 MS.gene25677:intergenic 45.0%
GGATGATGAATACTGTCCTG+AGG - 51704:4611-4630 MS.gene25677:CDS 45.0%
GTCTTCAACTTGGTCGTCAT+CGG + 51704:4172-4191 MS.gene25677:intergenic 45.0%
TCTTCAACTTGGTCGTCATC+GGG + 51704:4171-4190 MS.gene25677:intergenic 45.0%
TGGTTGGGAGTAACCAATGA+GGG + 51704:4206-4225 MS.gene25677:intergenic 45.0%
TTGGTTGGGAGTAACCAATG+AGG + 51704:4207-4226 MS.gene25677:intergenic 45.0%
! TAGTTAGCTTAGACCGACGA+AGG - 51704:4378-4397 MS.gene25677:intron 45.0%
!! TCGGTGTTGAAATCACTAGG+TGG - 51704:4469-4488 MS.gene25677:intron 45.0%
AGCCGCATTTGCAGATGTGT+AGG + 51704:4778-4797 MS.gene25677:intergenic 50.0%
CTGTAAACCTAATCGCGCAG+CGG - 51704:4796-4815 MS.gene25677:CDS 50.0%
GAATGTTACTGTCACGGAGG+AGG + 51704:4055-4074 MS.gene25677:intergenic 50.0%
GAGGAGGAAGATGGTGTGAA+TGG + 51704:4039-4058 MS.gene25677:intergenic 50.0%
GGCCTACACATCTGCAAATG+CGG - 51704:4773-4792 MS.gene25677:CDS 50.0%
GGTTGGGAGTAACCAATGAG+GGG + 51704:4205-4224 MS.gene25677:intergenic 50.0%
GTGAATGGGTACGAGTAGTG+TGG + 51704:4128-4147 MS.gene25677:intergenic 50.0%
GTGTGAATGGGATGATGCTG+CGG + 51704:4026-4045 MS.gene25677:intergenic 50.0%
GTTGGGAGTAACCAATGAGG+GGG + 51704:4204-4223 MS.gene25677:intergenic 50.0%
TGTGAATGGGATGATGCTGC+GGG + 51704:4025-4044 MS.gene25677:intergenic 50.0%
!! GAAAAGAGTACCTACCTGGG+AGG + 51704:4232-4251 MS.gene25677:intergenic 50.0%
!! TCGTCATCGGGTTCTGGAAA+TGG + 51704:4159-4178 MS.gene25677:intergenic 50.0%
ACCAATGAGGGGGAACACGA+AGG + 51704:4194-4213 MS.gene25677:intergenic 55.0%
ACCTTCGTGTTCCCCCTCAT+TGG - 51704:4190-4209 MS.gene25677:CDS 55.0%
ACTGTCACGGAGGAGGAAGA+TGG + 51704:4048-4067 MS.gene25677:intergenic 55.0%
ACTTGGTCGTCATCGGGTTC+TGG + 51704:4165-4184 MS.gene25677:intergenic 55.0%
CACATGTGTCCGTGGCCTTT+CGG - 51704:4450-4469 MS.gene25677:intron 55.0%
CGACGAAGGACACGTCAACA+CGG - 51704:4392-4411 MS.gene25677:intron 55.0%
GCTGCGGGGAGATAGAATGA+TGG + 51704:4010-4029 MS.gene25677:intergenic 55.0%
GTGAATGGGATGATGCTGCG+GGG + 51704:4024-4043 MS.gene25677:intergenic 55.0%
GTTCAATCCGCTGCGCGATT+AGG + 51704:4806-4825 MS.gene25677:intergenic 55.0%
! AGTACCTACCTGGGAGGCAT+TGG + 51704:4226-4245 MS.gene25677:intergenic 55.0%
! CGTGTTGACGTGTCCTTCGT+CGG + 51704:4394-4413 MS.gene25677:intergenic 55.0%
!! CTACCTGGGAGGCATTGGTT+GGG + 51704:4221-4240 MS.gene25677:intergenic 55.0%
ACTCCCAACCAATGCCTCCC+AGG - 51704:4215-4234 MS.gene25677:CDS 60.0%
CCAACCAATGCCTCCCAGGT+AGG - 51704:4219-4238 MS.gene25677:CDS 60.0%
!! CCTACCTGGGAGGCATTGGT+TGG + 51704:4222-4241 MS.gene25677:intergenic 60.0%


Chromosome Type Strat End Strand Name
51704 gene 3976 4845 3976 ID=MS.gene25677
51704 mRNA 3976 4845 3976 ID=MS.gene25677.t1;Parent=MS.gene25677
51704 exon 4585 4845 4585 ID=MS.gene25677.t1.exon1;Parent=MS.gene25677.t1
51704 CDS 4585 4845 4585 ID=cds.MS.gene25677.t1;Parent=MS.gene25677.t1
51704 exon 3976 4248 3976 ID=MS.gene25677.t1.exon2;Parent=MS.gene25677.t1
51704 CDS 3976 4248 3976 ID=cds.MS.gene25677.t1;Parent=MS.gene25677.t1
Gene Sequence

>MS.gene25677

ATGTCAAGCTTGAAATTGAATCTAGTTCATACCATCATTCTATCTCCCCGCAGCATCATCCCATTCACACCATCTTCCTCCTCCGTGACAGTAACATTCACTCACCACCAATACGCTTCTTCTTCATCTTCCTCTTCACCAAAAATTGTCCACACTACTCGTACCCATTCACATTCTCATCCATTTCCAGAACCCGATGACGACCAAGTTGAAGACCTTCGTGTTCCCCCTCATTGGTTACTCCCAACCAATGCCTCCCAGGTAGGTACTCTTTTCATTCATTCCTCTCAAATTTTAGAAACACGTAGTTGTACTTGTAGCTGAGATGTTCATGTTAGAATTGGTTACATATTGTTAAGGTAGTTACAATTGAGTTATGATTAGTTAAAAGTTAGTTAGAATTAGTTAGCTTAGACCGACGAAGGACACGTCAACACGGTAATAATTTGAGAAAACAGCATTGTTGAAAGTAATCACATGTGTCCGTGGCCTTTCGGTGTTGAAATCACTAGGTGGTTAGGATCACTATAAAAAAAATGTATCCTGCGCTCATTTATATCTTCATTAATTGTTTTTTTTTCCATTCACAATTATTAGGAATCAGAGTGGCTTAGGGTTACTTTACATAAATGGTTGGATGATGAATACTGTCCTGAGGAAACAAATGTGGAGATAAGCAGAGTTGCTGCCAAATCCTACTACAATTCTTTGATACGGAAACAAGCTGATTTAGCTGACATTTTGTTGAATATGGCACGTGAATTGGAAACCATTTCTTATAAGGAAAGTTTTCATGGGGCCTACACATCTGCAAATGCGGCTGTAAACCTAATCGCGCAGCGGATTGAACAATTCTGCCACCCAAATTGA

Protein sequence

>MS.gene25677.t1

MSSLKLNLVHTIILSPRSIIPFTPSSSSVTVTFTHHQYASSSSSSSPKIVHTTRTHSHSHPFPEPDDDQVEDLRVPPHWLLPTNASQESEWLRVTLHKWLDDEYCPEETNVEISRVAAKSYYNSLIRKQADLADILLNMARELETISYKESFHGAYTSANAAVNLIAQRIEQFCHPN