AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52799


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52799.t1 MTR_3g114850 98.802 167 2 0 1 167 1 167 3.88e-118 331
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52799.t1 AT1G20340 65.868 167 57 0 1 167 1 167 7.48e-73 216
MS.gene52799.t1 AT1G76100 64.327 171 57 2 1 167 1 171 1.28e-72 216
MS.gene52799.t1 AT1G76100 64.327 171 57 2 1 167 37 207 2.45e-72 216

Find 50 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTCAGGCATGGAAATTT+TGG 0.145348 3.1:+90902763 None:intergenic
CTTTGCTTGCGTTTGCCTTA+AGG 0.331286 3.1:+90903077 None:intergenic
GCGTTCACTGTGAAATTGTT+TGG 0.355945 3.1:+90902875 None:intergenic
TCCATCATTCACAGGCCTTA+AGG 0.363430 3.1:-90903092 MS.gene52799:CDS
CTTGCAGTTGAAGTGTTGCT+TGG 0.408427 3.1:-90902926 MS.gene52799:CDS
CAGCAACTCCAAAGTCTTTG+AGG 0.421507 3.1:+90902990 None:intergenic
AGAGCTTCCCTCAAAGACTT+TGG 0.423979 3.1:-90902998 MS.gene52799:CDS
GCATTGAGAAGATCTTCTTC+AGG 0.435974 3.1:+90902749 None:intergenic
GAAGATCTTCTCAATGCTCC+TGG 0.437678 3.1:-90902743 MS.gene52799:CDS
CAGCGTTACTTTGGATGCTA+AGG 0.446131 3.1:-90902711 MS.gene52799:CDS
GGCAATTGAGAAGTTGAAGT+TGG 0.447601 3.1:+90903034 None:intergenic
AGGTGAACAGTAGAATTTGT+AGG 0.459706 3.1:+90902685 None:intergenic
GGAGACTTACAGCGTTACTT+TGG 0.478413 3.1:-90902720 MS.gene52799:CDS
TGTTGCTTGGTGCTAGTGAT+GGG 0.479693 3.1:-90902913 MS.gene52799:CDS
GTGTTGCTTGGTGCTAGTGA+TGG 0.482949 3.1:-90902914 MS.gene52799:CDS
ACCTCACGCAGGAGCTGGTA+TGG 0.490163 3.1:-90902666 MS.gene52799:CDS
GCCTTAAGGCCTGTGAATGA+TGG 0.494140 3.1:+90903091 None:intergenic
ATTACATTCAAGAACAATGC+TGG 0.496557 3.1:-90902842 MS.gene52799:CDS
AGCATTGTTCTTGAATGTAA+TGG 0.506572 3.1:+90902844 None:intergenic
AAGATCTTCTCAATGCTCCT+GGG 0.518214 3.1:-90902742 MS.gene52799:CDS
AGCAACTCCAAAGTCTTTGA+GGG 0.521503 3.1:+90902991 None:intergenic
CTTCAACTTCTCAATTGCCA+AGG 0.523550 3.1:-90903030 MS.gene52799:CDS
TGTTCACCTCACGCAGGAGC+TGG 0.531287 3.1:-90902671 MS.gene52799:CDS
TTGGCTGCATCAACCCCGCT+TGG 0.532446 3.1:+90902782 None:intergenic
GAGAAGATCTTCTTCAGGCA+TGG 0.537563 3.1:+90902754 None:intergenic
CACGCAGGAGCTGGTATGGT+TGG 0.542139 3.1:-90902662 MS.gene52799:CDS
AACAATTTCACAGTGAACGC+TGG 0.547209 3.1:-90902872 MS.gene52799:CDS
TAACAATGCACTTGCAGCAG+TGG 0.551331 3.1:+90902961 None:intergenic
AGCAACGGTGGTGGAAGTAA+CGG 0.554653 3.1:+90903117 None:intergenic
CAAAGTTAATGCCATAGCTA+AGG 0.556491 3.1:-90903059 MS.gene52799:CDS
GAAGCTCTGACACAAAGCCT+TGG 0.567176 3.1:+90903013 None:intergenic
GTTGCTATTCCATCATTCAC+AGG 0.569968 3.1:-90903100 MS.gene52799:CDS
TGAAGTTGGAACCTTAGCTA+TGG 0.578592 3.1:+90903048 None:intergenic
GTTGCTTGGTGCTAGTGATG+GGG 0.579404 3.1:-90902912 MS.gene52799:CDS
AGCGTTACTTTGGATGCTAA+GGG 0.580511 3.1:-90902710 MS.gene52799:CDS
GTAACGCTGTAAGTCTCCCC+AGG 0.594723 3.1:+90902725 None:intergenic
TGATGGAATAGCAACGGTGG+TGG 0.597217 3.1:+90903108 None:intergenic
CAAGCAACACTTCAACTGCA+AGG 0.598151 3.1:+90902927 None:intergenic
TTGCTTGGTGCTAGTGATGG+GGG 0.603056 3.1:-90902911 MS.gene52799:CDS
ATGAAGACGAGATTCCAAGC+GGG 0.608107 3.1:-90902796 MS.gene52799:CDS
AAGCAACACTTCAACTGCAA+GGG 0.614894 3.1:+90902928 None:intergenic
ACCATACCAGCTCCTGCGTG+AGG 0.641555 3.1:+90902665 None:intergenic
TCATCGAAGATAACGTTGTG+AGG 0.653170 3.1:+90902815 None:intergenic
TGAAGACGAGATTCCAAGCG+GGG 0.662660 3.1:-90902795 MS.gene52799:CDS
AGATCTTCTCAATGCTCCTG+GGG 0.672138 3.1:-90902741 MS.gene52799:CDS
AACGGTGGTGGAAGTAACGG+TGG 0.682356 3.1:+90903120 None:intergenic
TTCTACTGTTCACCTCACGC+AGG 0.697342 3.1:-90902677 MS.gene52799:CDS
TGTGAATGATGGAATAGCAA+CGG 0.697370 3.1:+90903102 None:intergenic
GATGAAGACGAGATTCCAAG+CGG 0.752965 3.1:-90902797 MS.gene52799:CDS
GAATGATGGAATAGCAACGG+TGG 0.782143 3.1:+90903105 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGCATTGTTCTTGAATGTAA+TGG + chr3.1:90902926-90902945 None:intergenic 30.0%
ATTACATTCAAGAACAATGC+TGG - chr3.1:90902925-90902944 MS.gene52799:CDS 30.0%
AGGTGAACAGTAGAATTTGT+AGG + chr3.1:90903085-90903104 None:intergenic 35.0%
CAAAGTTAATGCCATAGCTA+AGG - chr3.1:90902708-90902727 MS.gene52799:CDS 35.0%
! TGTGAATGATGGAATAGCAA+CGG + chr3.1:90902668-90902687 None:intergenic 35.0%
! TTCTTCAGGCATGGAAATTT+TGG + chr3.1:90903007-90903026 None:intergenic 35.0%
AACAATTTCACAGTGAACGC+TGG - chr3.1:90902895-90902914 MS.gene52799:CDS 40.0%
AAGATCTTCTCAATGCTCCT+GGG - chr3.1:90903025-90903044 MS.gene52799:CDS 40.0%
AAGCAACACTTCAACTGCAA+GGG + chr3.1:90902842-90902861 None:intergenic 40.0%
AGCAACTCCAAAGTCTTTGA+GGG + chr3.1:90902779-90902798 None:intergenic 40.0%
CTTCAACTTCTCAATTGCCA+AGG - chr3.1:90902737-90902756 MS.gene52799:CDS 40.0%
GCATTGAGAAGATCTTCTTC+AGG + chr3.1:90903021-90903040 None:intergenic 40.0%
GCGTTCACTGTGAAATTGTT+TGG + chr3.1:90902895-90902914 None:intergenic 40.0%
GGCAATTGAGAAGTTGAAGT+TGG + chr3.1:90902736-90902755 None:intergenic 40.0%
TCATCGAAGATAACGTTGTG+AGG + chr3.1:90902955-90902974 None:intergenic 40.0%
TGAAGTTGGAACCTTAGCTA+TGG + chr3.1:90902722-90902741 None:intergenic 40.0%
! AGCGTTACTTTGGATGCTAA+GGG - chr3.1:90903057-90903076 MS.gene52799:CDS 40.0%
! GTTGCTATTCCATCATTCAC+AGG - chr3.1:90902667-90902686 MS.gene52799:CDS 40.0%
AGAGCTTCCCTCAAAGACTT+TGG - chr3.1:90902769-90902788 MS.gene52799:CDS 45.0%
AGATCTTCTCAATGCTCCTG+GGG - chr3.1:90903026-90903045 MS.gene52799:CDS 45.0%
ATGAAGACGAGATTCCAAGC+GGG - chr3.1:90902971-90902990 MS.gene52799:CDS 45.0%
CAAGCAACACTTCAACTGCA+AGG + chr3.1:90902843-90902862 None:intergenic 45.0%
CAGCAACTCCAAAGTCTTTG+AGG + chr3.1:90902780-90902799 None:intergenic 45.0%
CTTTGCTTGCGTTTGCCTTA+AGG + chr3.1:90902693-90902712 None:intergenic 45.0%
GAAGATCTTCTCAATGCTCC+TGG - chr3.1:90903024-90903043 MS.gene52799:CDS 45.0%
GAGAAGATCTTCTTCAGGCA+TGG + chr3.1:90903016-90903035 None:intergenic 45.0%
GATGAAGACGAGATTCCAAG+CGG - chr3.1:90902970-90902989 MS.gene52799:CDS 45.0%
GGAGACTTACAGCGTTACTT+TGG - chr3.1:90903047-90903066 MS.gene52799:CDS 45.0%
TAACAATGCACTTGCAGCAG+TGG + chr3.1:90902809-90902828 None:intergenic 45.0%
TCCATCATTCACAGGCCTTA+AGG - chr3.1:90902675-90902694 MS.gene52799:CDS 45.0%
! CAGCGTTACTTTGGATGCTA+AGG - chr3.1:90903056-90903075 MS.gene52799:CDS 45.0%
! GAATGATGGAATAGCAACGG+TGG + chr3.1:90902665-90902684 None:intergenic 45.0%
!! CTTGCAGTTGAAGTGTTGCT+TGG - chr3.1:90902841-90902860 MS.gene52799:CDS 45.0%
!! TGTTGCTTGGTGCTAGTGAT+GGG - chr3.1:90902854-90902873 MS.gene52799:CDS 45.0%
GCCTTAAGGCCTGTGAATGA+TGG + chr3.1:90902679-90902698 None:intergenic 50.0%
TGAAGACGAGATTCCAAGCG+GGG - chr3.1:90902972-90902991 MS.gene52799:CDS 50.0%
TGATGGAATAGCAACGGTGG+TGG + chr3.1:90902662-90902681 None:intergenic 50.0%
TTCTACTGTTCACCTCACGC+AGG - chr3.1:90903090-90903109 MS.gene52799:CDS 50.0%
! GAAGCTCTGACACAAAGCCT+TGG + chr3.1:90902757-90902776 None:intergenic 50.0%
!! AGCAACGGTGGTGGAAGTAA+CGG + chr3.1:90902653-90902672 None:intergenic 50.0%
!! GTGTTGCTTGGTGCTAGTGA+TGG - chr3.1:90902853-90902872 MS.gene52799:CDS 50.0%
!! GTTGCTTGGTGCTAGTGATG+GGG - chr3.1:90902855-90902874 MS.gene52799:CDS 50.0%
!! TTGCTTGGTGCTAGTGATGG+GGG - chr3.1:90902856-90902875 MS.gene52799:CDS 50.0%
GTAACGCTGTAAGTCTCCCC+AGG + chr3.1:90903045-90903064 None:intergenic 55.0%
! TGGTGCTAGTGATGGGGGTT+TGG - chr3.1:90902861-90902880 MS.gene52799:CDS 55.0%
!! AACGGTGGTGGAAGTAACGG+TGG + chr3.1:90902650-90902669 None:intergenic 55.0%
ACCATACCAGCTCCTGCGTG+AGG + chr3.1:90903105-90903124 None:intergenic 60.0%
ACCTCACGCAGGAGCTGGTA+TGG - chr3.1:90903101-90903120 MS.gene52799:CDS 60.0%
CACGCAGGAGCTGGTATGGT+TGG - chr3.1:90903105-90903124 MS.gene52799:CDS 60.0%
TGTTCACCTCACGCAGGAGC+TGG - chr3.1:90903096-90903115 MS.gene52799:CDS 60.0%
TTGGCTGCATCAACCCCGCT+TGG + chr3.1:90902988-90903007 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 90902643 90903146 90902643 ID=MS.gene52799
chr3.1 mRNA 90902643 90903146 90902643 ID=MS.gene52799.t1;Parent=MS.gene52799
chr3.1 exon 90902643 90903146 90902643 ID=MS.gene52799.t1.exon1;Parent=MS.gene52799.t1
chr3.1 CDS 90902643 90903146 90902643 ID=cds.MS.gene52799.t1;Parent=MS.gene52799.t1
Gene Sequence

>MS.gene52799

ATGGCCACCGTTACTTCCACCACCGTTGCTATTCCATCATTCACAGGCCTTAAGGCAAACGCAAGCAAAGTTAATGCCATAGCTAAGGTTCCAACTTCAACTTCTCAATTGCCAAGGCTTTGTGTCAGAGCTTCCCTCAAAGACTTTGGAGTTGCTGTTGTTGCCACTGCTGCAAGTGCATTGTTAGCTAGCAATGCCCTTGCAGTTGAAGTGTTGCTTGGTGCTAGTGATGGGGGTTTGGCTTTTGTTCCAAACAATTTCACAGTGAACGCTGGAGACACCATTACATTCAAGAACAATGCTGGTTTTCCTCACAACGTTATCTTCGATGAAGACGAGATTCCAAGCGGGGTTGATGCAGCCAAAATTTCCATGCCTGAAGAAGATCTTCTCAATGCTCCTGGGGAGACTTACAGCGTTACTTTGGATGCTAAGGGAACCTACAAATTCTACTGTTCACCTCACGCAGGAGCTGGTATGGTTGGACAAGTCACTGTTAATTAA

Protein sequence

>MS.gene52799.t1

MATVTSTTVAIPSFTGLKANASKVNAIAKVPTSTSQLPRLCVRASLKDFGVAVVATAASALLASNALAVEVLLGASDGGLAFVPNNFTVNAGDTITFKNNAGFPHNVIFDEDEIPSGVDAAKISMPEEDLLNAPGETYSVTLDAKGTYKFYCSPHAGAGMVGQVTVN