AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62792


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62792.t1 MTR_4g103330 95.968 248 10 0 1 248 1 248 1.89e-155 432
MS.gene62792.t1 MTR_7g117900 28.111 217 129 9 6 215 29 225 3.02e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62792.t1 AT4G09650 55.600 250 92 3 1 248 1 233 4.72e-90 266

Find 69 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGCGTAGCTGGCGGCGGC+TGG 0.162799 8.1:+20882181 None:intergenic
GGTGAGGGTTGTTGTGGTTT+TGG 0.164088 8.1:+20882324 None:intergenic
ACCGTCAGGTATGGGAATTC+TGG 0.172359 8.1:-20881734 MS.gene62792:CDS
ATGAGGTTGAGGATGGGTTT+TGG 0.198972 8.1:+20882301 None:intergenic
GGTGCGGCGGGGTTTGGTTT+TGG 0.225649 8.1:+20882237 None:intergenic
TGCGCCACCGCCGGTGCGGC+GGG 0.238128 8.1:+20882225 None:intergenic
TTGGTGGAGTCTTCGATAAT+TGG 0.238527 8.1:+20882046 None:intergenic
AATTGCTGCTCAACTTGATT+TGG 0.271511 8.1:-20881669 MS.gene62792:CDS
TGGCGTAGCTGGCGGCGGCT+GGG 0.280536 8.1:+20882182 None:intergenic
CGCACCGGCGGTGGCGCACT+TGG 0.293106 8.1:-20882220 MS.gene62792:CDS
AACTGCTCTTGATCCAAGTT+TGG 0.310216 8.1:-20881768 MS.gene62792:CDS
ACCGCCGGTGCGGCGGGGTT+TGG 0.319431 8.1:+20882231 None:intergenic
GAAATTGGAGTCACAGCATT+TGG 0.323866 8.1:-20881846 MS.gene62792:CDS
AGGTGAAGGTGGTGCGGGAG+AGG 0.326146 8.1:+20882278 None:intergenic
TTTGGCGCAGATTGCGAAAC+AGG 0.327217 8.1:-20881828 MS.gene62792:CDS
GTCGAGGGTGTTGTTGGATT+TGG 0.360334 8.1:+20882141 None:intergenic
GAGAAATAGTCGAACACCTT+GGG 0.363815 8.1:+20882073 None:intergenic
GTGCGCCACCGCCGGTGCGG+CGG 0.369329 8.1:+20882224 None:intergenic
ATTGCTGCTCAACTTGATTT+GGG 0.372073 8.1:-20881668 MS.gene62792:CDS
CCGTCAGGTATGGGAATTCT+GGG 0.374285 8.1:-20881733 MS.gene62792:CDS
GGTGTCTTCAGTGGTGAAAT+TGG 0.383089 8.1:-20881861 MS.gene62792:CDS
GGCTAATGCGGTGGCGTAGC+TGG 0.387942 8.1:+20882171 None:intergenic
AGCGATGAGTTTGCGTTTGG+TGG 0.395419 8.1:+20882030 None:intergenic
GTAAAACCCGCCACCAAACT+TGG 0.397977 8.1:+20881755 None:intergenic
TGGTGGAGTCTTCGATAATT+GGG 0.398456 8.1:+20882047 None:intergenic
GGCGTAGCTGGCGGCGGCTG+GGG 0.401952 8.1:+20882183 None:intergenic
GTTGTTGGATTTGGCTAAAT+CGG 0.409703 8.1:+20882150 None:intergenic
AAACCCCGCCGCACCGGCGG+TGG 0.420052 8.1:-20882229 MS.gene62792:CDS
TTCAGCGATGAGTTTGCGTT+TGG 0.420804 8.1:+20882027 None:intergenic
GGAGAGGATGAGGTTGAGGA+TGG 0.424030 8.1:+20882294 None:intergenic
GGTGGCGTCGAGGGTGTTGT+TGG 0.434585 8.1:+20882135 None:intergenic
TGGGGAGGTGAAGGTGGTGC+GGG 0.436035 8.1:+20882273 None:intergenic
TGCGGTGGCGTAGCTGGCGG+CGG 0.438767 8.1:+20882177 None:intergenic
TTTGTCGATGTCAGCGGTGG+TGG 0.445558 8.1:+20882117 None:intergenic
AGGTGGTGCGGGAGAGGATG+AGG 0.454682 8.1:+20882284 None:intergenic
GTCAGCGGTGGTGGCGTCGA+GGG 0.465818 8.1:+20882126 None:intergenic
TTGGGGAGGTGAAGGTGGTG+CGG 0.467344 8.1:+20882272 None:intergenic
GGAGCCAAGTGCGCCACCGC+CGG 0.467362 8.1:+20882216 None:intergenic
TGCGGGAGAGGATGAGGTTG+AGG 0.468450 8.1:+20882290 None:intergenic
AAAACCAAACCCCGCCGCAC+CGG 0.470667 8.1:-20882235 MS.gene62792:CDS
CTTGATCCAAGTTTGGTGGC+GGG 0.474377 8.1:-20881761 MS.gene62792:CDS
CAAGTGCGCCACCGCCGGTG+CGG 0.475361 8.1:+20882221 None:intergenic
TTTGGAGTAGATTGAGGCTG+TGG 0.484590 8.1:+20882354 None:intergenic
TGCTCTTGATCCAAGTTTGG+TGG 0.485731 8.1:-20881765 MS.gene62792:CDS
GATTGGTTTGGAGTAGATTG+AGG 0.502890 8.1:+20882348 None:intergenic
TCTTGATCCAAGTTTGGTGG+CGG 0.512469 8.1:-20881762 MS.gene62792:CDS
TTCTGTTTGCTTCGATGAGG+AGG 0.515130 8.1:+20881957 None:intergenic
TTGCTTCGATGAGGAGGTTG+AGG 0.520100 8.1:+20881963 None:intergenic
ACCAAACCCCGCCGCACCGG+CGG 0.521926 8.1:-20882232 MS.gene62792:CDS
GCGCCACCGCCGGTGCGGCG+GGG 0.523246 8.1:+20882226 None:intergenic
GAGAGGATGAGGTTGAGGAT+GGG 0.530175 8.1:+20882295 None:intergenic
GGAGTCTTCGATAATTGGGC+TGG 0.533217 8.1:+20882051 None:intergenic
GGAGAAATAGTCGAACACCT+TGG 0.545934 8.1:+20882072 None:intergenic
CGATTCTGTTTGCTTCGATG+AGG 0.552223 8.1:+20881954 None:intergenic
TAATGCGGTGGCGTAGCTGG+CGG 0.559050 8.1:+20882174 None:intergenic
GGCTAAATCGGCTAATGCGG+TGG 0.571556 8.1:+20882162 None:intergenic
GCTTGTTGTGGTGTCTTCAG+TGG 0.590381 8.1:-20881870 MS.gene62792:CDS
TGTCAGCGGTGGTGGCGTCG+AGG 0.591340 8.1:+20882125 None:intergenic
GGCGGCTGGGGAGTTCATTG+TGG 0.606376 8.1:+20882195 None:intergenic
AGAACTCTTCACTGACCCCA+AGG 0.615212 8.1:-20882089 MS.gene62792:CDS
CGAATTCTCTCACGATGTCG+AGG 0.616657 8.1:+20881924 None:intergenic
TTTGGCTAAATCGGCTAATG+CGG 0.625107 8.1:+20882159 None:intergenic
AACCGACACAGAGCTTGTTG+TGG 0.631106 8.1:-20881882 MS.gene62792:CDS
TCACAGGGGCGAAGAATGTG+AGG 0.634295 8.1:-20881796 MS.gene62792:CDS
GAGTGTTAAGAAGAAACTTG+AGG 0.637701 8.1:-20881693 MS.gene62792:CDS
CACCACAACAAGCTCTGTGT+CGG 0.640807 8.1:+20881880 None:intergenic
CCCAGAATTCCCATACCTGA+CGG 0.678407 8.1:+20881733 None:intergenic
TGTGTGTGGTTGGAAGCTAG+AGG 0.683601 8.1:+20881994 None:intergenic
AGAAATAGTCGAACACCTTG+GGG 0.687282 8.1:+20882074 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTTGGAGAGTTTGAGTTTT+GGG + chr8.1:20881768-20881787 None:intergenic 30.0%
AAACAGGTTCAAAAACTCAC+AGG - chr8.1:20882207-20882226 MS.gene62792:CDS 35.0%
AACAGGTTCAAAAACTCACA+GGG - chr8.1:20882208-20882227 MS.gene62792:CDS 35.0%
AATTGCTGCTCAACTTGATT+TGG - chr8.1:20882350-20882369 MS.gene62792:CDS 35.0%
ATTGCTGCTCAACTTGATTT+GGG - chr8.1:20882351-20882370 MS.gene62792:CDS 35.0%
GAGTGTTAAGAAGAAACTTG+AGG - chr8.1:20882326-20882345 MS.gene62792:CDS 35.0%
GTTGTTGGATTTGGCTAAAT+CGG + chr8.1:20881872-20881891 None:intergenic 35.0%
!! TTTGGAGAGTTTGAGTTTTG+GGG + chr8.1:20881767-20881786 None:intergenic 35.0%
!!! GTTTTGGAGAGTTTGAGTTT+TGG + chr8.1:20881769-20881788 None:intergenic 35.0%
AACTGCTCTTGATCCAAGTT+TGG - chr8.1:20882251-20882270 MS.gene62792:CDS 40.0%
ACAGGTTCAAAAACTCACAG+GGG - chr8.1:20882209-20882228 MS.gene62792:CDS 40.0%
AGAAATAGTCGAACACCTTG+GGG + chr8.1:20881948-20881967 None:intergenic 40.0%
GAAATTGGAGTCACAGCATT+TGG - chr8.1:20882173-20882192 MS.gene62792:CDS 40.0%
GAGAAATAGTCGAACACCTT+GGG + chr8.1:20881949-20881968 None:intergenic 40.0%
TGGTGGAGTCTTCGATAATT+GGG + chr8.1:20881975-20881994 None:intergenic 40.0%
TTGGTGGAGTCTTCGATAAT+TGG + chr8.1:20881976-20881995 None:intergenic 40.0%
TTTGGCTAAATCGGCTAATG+CGG + chr8.1:20881863-20881882 None:intergenic 40.0%
! TTCGATTTTGTCGATGTCAG+CGG + chr8.1:20881911-20881930 None:intergenic 40.0%
!! GATTGGTTTGGAGTAGATTG+AGG + chr8.1:20881674-20881693 None:intergenic 40.0%
!! TTGAGGAAGTTTTGTGTGTG+TGG + chr8.1:20882042-20882061 None:intergenic 40.0%
CGATTCTGTTTGCTTCGATG+AGG + chr8.1:20882068-20882087 None:intergenic 45.0%
GGAGAAATAGTCGAACACCT+TGG + chr8.1:20881950-20881969 None:intergenic 45.0%
GGTGTCTTCAGTGGTGAAAT+TGG - chr8.1:20882158-20882177 MS.gene62792:CDS 45.0%
TGCTCTTGATCCAAGTTTGG+TGG - chr8.1:20882254-20882273 MS.gene62792:CDS 45.0%
TTCAGCGATGAGTTTGCGTT+TGG + chr8.1:20881995-20882014 None:intergenic 45.0%
TTCTGTTTGCTTCGATGAGG+AGG + chr8.1:20882065-20882084 None:intergenic 45.0%
! GTGAGGGTTGTTGTGGTTTT+GGG + chr8.1:20881697-20881716 None:intergenic 45.0%
! TCTTGATCCAAGTTTGGTGG+CGG - chr8.1:20882257-20882276 MS.gene62792:CDS 45.0%
! TTTGGAGTAGATTGAGGCTG+TGG + chr8.1:20881668-20881687 None:intergenic 45.0%
!! ATGAGGTTGAGGATGGGTTT+TGG + chr8.1:20881721-20881740 None:intergenic 45.0%
!! GGAAGTTTTGTGTGTGTGGT+TGG + chr8.1:20882038-20882057 None:intergenic 45.0%
!! TGAGGGTTGTTGTGGTTTTG+GGG + chr8.1:20881696-20881715 None:intergenic 45.0%
!! TGAGGTTGAGGATGGGTTTT+GGG + chr8.1:20881720-20881739 None:intergenic 45.0%
!!! GTTGTTGTGGTTTTGGGGAT+TGG + chr8.1:20881691-20881710 None:intergenic 45.0%
!!! TGTGGTTTTGGGGATTGGTT+TGG + chr8.1:20881686-20881705 None:intergenic 45.0%
!!! TTTGAGTTTTGGGGAGGTGA+AGG + chr8.1:20881758-20881777 None:intergenic 45.0%
ACCGTCAGGTATGGGAATTC+TGG - chr8.1:20882285-20882304 MS.gene62792:CDS 50.0%
AGAACTCTTCACTGACCCCA+AGG - chr8.1:20881930-20881949 MS.gene62792:CDS 50.0%
AGCGATGAGTTTGCGTTTGG+TGG + chr8.1:20881992-20882011 None:intergenic 50.0%
CACCACAACAAGCTCTGTGT+CGG + chr8.1:20882142-20882161 None:intergenic 50.0%
CCCAGAATTCCCATACCTGA+CGG + chr8.1:20882289-20882308 None:intergenic 50.0%
CCGTCAGGTATGGGAATTCT+GGG - chr8.1:20882286-20882305 MS.gene62792:CDS 50.0%
CGAATTCTCTCACGATGTCG+AGG + chr8.1:20882098-20882117 None:intergenic 50.0%
GAGAGGATGAGGTTGAGGAT+GGG + chr8.1:20881727-20881746 None:intergenic 50.0%
GGAGTCTTCGATAATTGGGC+TGG + chr8.1:20881971-20881990 None:intergenic 50.0%
GTAAAACCCGCCACCAAACT+TGG + chr8.1:20882267-20882286 None:intergenic 50.0%
TGTGTGTGGTTGGAAGCTAG+AGG + chr8.1:20882028-20882047 None:intergenic 50.0%
TTGCTTCGATGAGGAGGTTG+AGG + chr8.1:20882059-20882078 None:intergenic 50.0%
TTTGGCGCAGATTGCGAAAC+AGG - chr8.1:20882191-20882210 MS.gene62792:CDS 50.0%
! CGGGTTTTACCGTCAGGTAT+GGG - chr8.1:20882277-20882296 MS.gene62792:CDS 50.0%
! CTTGATCCAAGTTTGGTGGC+GGG - chr8.1:20882258-20882277 MS.gene62792:CDS 50.0%
! GATTTTGTCGATGTCAGCGG+TGG + chr8.1:20881908-20881927 None:intergenic 50.0%
! GGTGAGGGTTGTTGTGGTTT+TGG + chr8.1:20881698-20881717 None:intergenic 50.0%
!! AACCGACACAGAGCTTGTTG+TGG - chr8.1:20882137-20882156 MS.gene62792:CDS 50.0%
!! GAGGTTGAGGATGGGTTTTG+GGG + chr8.1:20881719-20881738 None:intergenic 50.0%
!! GCTTGTTGTGGTGTCTTCAG+TGG - chr8.1:20882149-20882168 MS.gene62792:CDS 50.0%
!! GTCGAGGGTGTTGTTGGATT+TGG + chr8.1:20881881-20881900 None:intergenic 50.0%
!!! GGAGAGTTTGAGTTTTGGGG+AGG + chr8.1:20881764-20881783 None:intergenic 50.0%
!!! TTTTGGGGTGAGGGTTGTTG+TGG + chr8.1:20881704-20881723 None:intergenic 50.0%
GGAGAGGATGAGGTTGAGGA+TGG + chr8.1:20881728-20881747 None:intergenic 55.0%
GGCTAAATCGGCTAATGCGG+TGG + chr8.1:20881860-20881879 None:intergenic 55.0%
TCACAGGGGCGAAGAATGTG+AGG - chr8.1:20882223-20882242 MS.gene62792:CDS 55.0%
TTTGTCGATGTCAGCGGTGG+TGG + chr8.1:20881905-20881924 None:intergenic 55.0%
! GCGGGTTTTACCGTCAGGTA+TGG - chr8.1:20882276-20882295 MS.gene62792:CDS 55.0%
!!! GAGGATGGGTTTTGGGGTGA+GGG + chr8.1:20881713-20881732 None:intergenic 55.0%
!!! GAGTTTTGGGGAGGTGAAGG+TGG + chr8.1:20881755-20881774 None:intergenic 55.0%
!!! TGAGGATGGGTTTTGGGGTG+AGG + chr8.1:20881714-20881733 None:intergenic 55.0%
AAAACCAAACCCCGCCGCAC+CGG - chr8.1:20881784-20881803 MS.gene62792:CDS 60.0%
TAATGCGGTGGCGTAGCTGG+CGG + chr8.1:20881848-20881867 None:intergenic 60.0%
TGCGGGAGAGGATGAGGTTG+AGG + chr8.1:20881732-20881751 None:intergenic 60.0%
TTGGGGAGGTGAAGGTGGTG+CGG + chr8.1:20881750-20881769 None:intergenic 60.0%
!!! TGGTGGCGGGTTTTACCGTC+AGG - chr8.1:20882271-20882290 MS.gene62792:CDS 60.0%
AGGTGAAGGTGGTGCGGGAG+AGG + chr8.1:20881744-20881763 None:intergenic 65.0%
AGGTGGTGCGGGAGAGGATG+AGG + chr8.1:20881738-20881757 None:intergenic 65.0%
GGCGGCTGGGGAGTTCATTG+TGG + chr8.1:20881827-20881846 None:intergenic 65.0%
GGCTAATGCGGTGGCGTAGC+TGG + chr8.1:20881851-20881870 None:intergenic 65.0%
TGGGGAGGTGAAGGTGGTGC+GGG + chr8.1:20881749-20881768 None:intergenic 65.0%
!! GGTGCGGCGGGGTTTGGTTT+TGG + chr8.1:20881785-20881804 None:intergenic 65.0%
!! GGTGGCGTCGAGGGTGTTGT+TGG + chr8.1:20881887-20881906 None:intergenic 65.0%
! GTCAGCGGTGGTGGCGTCGA+GGG + chr8.1:20881896-20881915 None:intergenic 70.0%
! TGTCAGCGGTGGTGGCGTCG+AGG + chr8.1:20881897-20881916 None:intergenic 70.0%
ACCAAACCCCGCCGCACCGG+CGG - chr8.1:20881787-20881806 MS.gene62792:CDS 75.0%
CAAGTGCGCCACCGCCGGTG+CGG + chr8.1:20881801-20881820 None:intergenic 75.0%
GGAGCCAAGTGCGCCACCGC+CGG + chr8.1:20881806-20881825 None:intergenic 75.0%
TGCGGTGGCGTAGCTGGCGG+CGG + chr8.1:20881845-20881864 None:intergenic 75.0%
TGGCGTAGCTGGCGGCGGCT+GGG + chr8.1:20881840-20881859 None:intergenic 75.0%
! ACCGCCGGTGCGGCGGGGTT+TGG + chr8.1:20881791-20881810 None:intergenic 80.0%
! GGCGTAGCTGGCGGCGGCTG+GGG + chr8.1:20881839-20881858 None:intergenic 80.0%
! GTGGCGTAGCTGGCGGCGGC+TGG + chr8.1:20881841-20881860 None:intergenic 80.0%
!! AAACCCCGCCGCACCGGCGG+TGG - chr8.1:20881790-20881809 MS.gene62792:CDS 80.0%
!! CGCACCGGCGGTGGCGCACT+TGG - chr8.1:20881799-20881818 MS.gene62792:CDS 80.0%
! GTGCGCCACCGCCGGTGCGG+CGG + chr8.1:20881798-20881817 None:intergenic 85.0%
! TGCGCCACCGCCGGTGCGGC+GGG + chr8.1:20881797-20881816 None:intergenic 85.0%
! GCGCCACCGCCGGTGCGGCG+GGG + chr8.1:20881796-20881815 None:intergenic 90.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 20881646 20882395 20881646 ID=MS.gene62792
chr8.1 mRNA 20881646 20882395 20881646 ID=MS.gene62792.t1;Parent=MS.gene62792
chr8.1 exon 20881646 20882395 20881646 ID=MS.gene62792.t1.exon1;Parent=MS.gene62792.t1
chr8.1 CDS 20881646 20882395 20881646 ID=cds.MS.gene62792.t1;Parent=MS.gene62792.t1
Gene Sequence

>MS.gene62792

ATGGCGTCTCTACAACACACCACAGCCTCAATCTACTCCAAACCAATCCCCAAAACCACAACAACCCTCACCCCAAAACCCATCCTCAACCTCATCCTCTCCCGCACCACCTTCACCTCCCCAAAACTCAAACTCTCCAAAACCAAACCCCGCCGCACCGGCGGTGGCGCACTTGGCTCCACAATGAACTCCCCAGCCGCCGCCAGCTACGCCACCGCATTAGCCGATTTAGCCAAATCCAACAACACCCTCGACGCCACCACCGCTGACATCGACAAAATCGAAGAACTCTTCACTGACCCCAAGGTGTTCGACTATTTCTCCAGCCCAATTATCGAAGACTCCACCAAACGCAAACTCATCGCTGAATTCGCTACTTCCTCTAGCTTCCAACCACACACACAAAACTTCCTCAACCTCCTCATCGAAGCAAACAGAATCGACATCATCCTCGACATCGTGAGAGAATTCGAACTTCATTACAATACTCTAACCGACACAGAGCTTGTTGTGGTGTCTTCAGTGGTGAAATTGGAGTCACAGCATTTGGCGCAGATTGCGAAACAGGTTCAAAAACTCACAGGGGCGAAGAATGTGAGGATTAAAACTGCTCTTGATCCAAGTTTGGTGGCGGGTTTTACCGTCAGGTATGGGAATTCTGGGTCTAAGTTTATTGATATGAGTGTTAAGAAGAAACTTGAGGAAATTGCTGCTCAACTTGATTTGGGTGATATCAAACTTGCTGCATGA

Protein sequence

>MS.gene62792.t1

MASLQHTTASIYSKPIPKTTTTLTPKPILNLILSRTTFTSPKLKLSKTKPRRTGGGALGSTMNSPAAASYATALADLAKSNNTLDATTADIDKIEELFTDPKVFDYFSSPIIEDSTKRKLIAEFATSSSFQPHTQNFLNLLIEANRIDIILDIVREFELHYNTLTDTELVVVSSVVKLESQHLAQIAKQVQKLTGAKNVRIKTALDPSLVAGFTVRYGNSGSKFIDMSVKKKLEEIAAQLDLGDIKLAA