AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09720


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09720.t1 MTR_5g013300 75.781 128 23 3 1 125 1 123 9.25e-62 189
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 38 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTCGACCTCAATTTCA+TGG 0.303030 5.3:+6371141 MS.gene09720:CDS
AACTGAAGGAGAACAGGTTT+TGG 0.311686 5.3:+6371286 MS.gene09720:CDS
GGCTTCCCAAGTATTTCTTC+AGG 0.325939 5.3:+6370899 MS.gene09720:CDS
GTAACTTACCCTTCATTTCC+AGG 0.329154 5.3:+6371117 MS.gene09720:CDS
TGTTAAGCTTGGTGATGTTA+TGG 0.331706 5.3:+6371355 MS.gene09720:CDS
ATTTGAAACAGCCTCGGAAT+TGG 0.345574 5.3:-6371216 None:intergenic
GAAGAGAAACCTAGCTTTGT+TGG 0.359069 5.3:-6371065 None:intergenic
GTTAAGCTTGGTGATGTTAT+GGG 0.359187 5.3:+6371356 MS.gene09720:CDS
TCAGCATTATCCGAAGCTTC+AGG 0.367212 5.3:-6371251 None:intergenic
TTTGTTGGAGGACATTGATT+AGG 0.374475 5.3:-6371050 None:intergenic
GGATAATGCTGATGAAGTTT+CGG 0.394551 5.3:+6371262 MS.gene09720:CDS
TTCGGAAACTGAAGGAGAAC+AGG 0.400877 5.3:+6371280 MS.gene09720:CDS
CGAAGAAGCCCTGGAAATGA+AGG 0.478400 5.3:-6371126 None:intergenic
TGATGAAAATCCTGAAGCTT+CGG 0.493188 5.3:+6371241 MS.gene09720:CDS
ACGAGTTTGTCTAGGCGGCT+TGG 0.497233 5.3:-6371333 None:intergenic
CAATGTCCTCCAACAAAGCT+AGG 0.507926 5.3:+6371056 MS.gene09720:CDS
TTTGGATTCTGCATCTGAGG+TGG 0.512535 5.3:+6371304 MS.gene09720:CDS
ATCAACATTTGAAACAGCCT+CGG 0.532181 5.3:-6371222 None:intergenic
GGATTCTGCATCTGAGGTGG+AGG 0.532596 5.3:+6371307 MS.gene09720:CDS
ACGAGAAGCGATGGTGGAAG+CGG 0.542669 5.3:-6371096 None:intergenic
TAACTTACCCTTCATTTCCA+GGG 0.543085 5.3:+6371118 MS.gene09720:CDS
TTAAGCTTGGTGATGTTATG+GGG 0.548890 5.3:+6371357 MS.gene09720:CDS
AATTGAGGTCGAAGAAGCCC+TGG 0.553413 5.3:-6371135 None:intergenic
ATTACAGCGCCATGAAATTG+AGG 0.558367 5.3:-6371150 None:intergenic
GAGAAACCTAGCTTTGTTGG+AGG 0.558664 5.3:-6371062 None:intergenic
TCAGCTCTCACAATAAAACT+AGG 0.565579 5.3:-6371191 None:intergenic
AGACAAACTCGTGTTAAGCT+TGG 0.574922 5.3:+6371344 MS.gene09720:CDS
AGCTTAACACGAGTTTGTCT+AGG 0.586483 5.3:-6371341 None:intergenic
TTAATAACAGTATAGAGTCA+TGG 0.599601 5.3:-6371394 None:intergenic
GGGTAAGTTACGAGAAGCGA+TGG 0.599796 5.3:-6371105 None:intergenic
AAACTAGGTTTAGCAATGAG+AGG 0.610020 5.3:-6371176 None:intergenic
GATGAAGTTTCGGAAACTGA+AGG 0.614399 5.3:+6371272 MS.gene09720:CDS
GAGAGCTGATTCCAATTCCG+AGG 0.627170 5.3:+6371205 MS.gene09720:CDS
TAAGTTACGAGAAGCGATGG+TGG 0.627813 5.3:-6371102 None:intergenic
CTAGGTTTAGCAATGAGAGG+AGG 0.642127 5.3:-6371173 None:intergenic
TTAACACGAGTTTGTCTAGG+CGG 0.648787 5.3:-6371338 None:intergenic
TAAGCTTGGTGATGTTATGG+GGG 0.663709 5.3:+6371358 MS.gene09720:CDS
GAAGAAGCCCTGGAAATGAA+GGG 0.680632 5.3:-6371125 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTATTTGTTACAATGAAT+TGG - chr5.3:6370961-6370980 None:intergenic 20.0%
! AAAAAACCTGAAGAAATACT+TGG - chr5.3:6370908-6370927 None:intergenic 25.0%
! AAAAACCTGAAGAAATACTT+GGG - chr5.3:6370907-6370926 None:intergenic 25.0%
! TTAATAACAGTATAGAGTCA+TGG - chr5.3:6371397-6371416 None:intergenic 25.0%
TGGATAATAATGAGATGAGA+GGG - chr5.3:6370941-6370960 None:intergenic 30.0%
TTGGATAATAATGAGATGAG+AGG - chr5.3:6370942-6370961 None:intergenic 30.0%
AAACTAGGTTTAGCAATGAG+AGG - chr5.3:6371179-6371198 None:intergenic 35.0%
GGATAATGCTGATGAAGTTT+CGG + chr5.3:6371262-6371281 MS.gene09720:CDS 35.0%
TAACTTACCCTTCATTTCCA+GGG + chr5.3:6371118-6371137 MS.gene09720:CDS 35.0%
TCAGCTCTCACAATAAAACT+AGG - chr5.3:6371194-6371213 None:intergenic 35.0%
TGATGAAAATCCTGAAGCTT+CGG + chr5.3:6371241-6371260 MS.gene09720:CDS 35.0%
! ATCAACATTTGAAACAGCCT+CGG - chr5.3:6371225-6371244 None:intergenic 35.0%
! TTTGTTGGAGGACATTGATT+AGG - chr5.3:6371053-6371072 None:intergenic 35.0%
!! GTTAAGCTTGGTGATGTTAT+GGG + chr5.3:6371356-6371375 MS.gene09720:CDS 35.0%
!! TGTTAAGCTTGGTGATGTTA+TGG + chr5.3:6371355-6371374 MS.gene09720:CDS 35.0%
!! TTAAGCTTGGTGATGTTATG+GGG + chr5.3:6371357-6371376 MS.gene09720:CDS 35.0%
AGACAAACTCGTGTTAAGCT+TGG + chr5.3:6371344-6371363 MS.gene09720:CDS 40.0%
AGCTTAACACGAGTTTGTCT+AGG - chr5.3:6371344-6371363 None:intergenic 40.0%
ATTACAGCGCCATGAAATTG+AGG - chr5.3:6371153-6371172 None:intergenic 40.0%
CTTCTTCGACCTCAATTTCA+TGG + chr5.3:6371141-6371160 MS.gene09720:CDS 40.0%
GATGAAGTTTCGGAAACTGA+AGG + chr5.3:6371272-6371291 MS.gene09720:CDS 40.0%
GTAACTTACCCTTCATTTCC+AGG + chr5.3:6371117-6371136 MS.gene09720:CDS 40.0%
TTAACACGAGTTTGTCTAGG+CGG - chr5.3:6371341-6371360 None:intergenic 40.0%
! AACTGAAGGAGAACAGGTTT+TGG + chr5.3:6371286-6371305 MS.gene09720:CDS 40.0%
! ATTTGAAACAGCCTCGGAAT+TGG - chr5.3:6371219-6371238 None:intergenic 40.0%
!! GAAGAGAAACCTAGCTTTGT+TGG - chr5.3:6371068-6371087 None:intergenic 40.0%
!! TAAGCTTGGTGATGTTATGG+GGG + chr5.3:6371358-6371377 MS.gene09720:CDS 40.0%
CAATGTCCTCCAACAAAGCT+AGG + chr5.3:6371056-6371075 MS.gene09720:CDS 45.0%
CTAGGTTTAGCAATGAGAGG+AGG - chr5.3:6371176-6371195 None:intergenic 45.0%
GAAGAAGCCCTGGAAATGAA+GGG - chr5.3:6371128-6371147 None:intergenic 45.0%
GGCTTCCCAAGTATTTCTTC+AGG + chr5.3:6370899-6370918 MS.gene09720:CDS 45.0%
TAAGTTACGAGAAGCGATGG+TGG - chr5.3:6371105-6371124 None:intergenic 45.0%
TCAGCATTATCCGAAGCTTC+AGG - chr5.3:6371254-6371273 None:intergenic 45.0%
TTCGGAAACTGAAGGAGAAC+AGG + chr5.3:6371280-6371299 MS.gene09720:CDS 45.0%
TTTGGATTCTGCATCTGAGG+TGG + chr5.3:6371304-6371323 MS.gene09720:CDS 45.0%
!! GAGAAACCTAGCTTTGTTGG+AGG - chr5.3:6371065-6371084 None:intergenic 45.0%
!!! GGTTTTGGATTCTGCATCTG+AGG + chr5.3:6371301-6371320 MS.gene09720:CDS 45.0%
AATTGAGGTCGAAGAAGCCC+TGG - chr5.3:6371138-6371157 None:intergenic 50.0%
CGAAGAAGCCCTGGAAATGA+AGG - chr5.3:6371129-6371148 None:intergenic 50.0%
GGGTAAGTTACGAGAAGCGA+TGG - chr5.3:6371108-6371127 None:intergenic 50.0%
! GAGAGCTGATTCCAATTCCG+AGG + chr5.3:6371205-6371224 MS.gene09720:CDS 50.0%
ACGAGTTTGTCTAGGCGGCT+TGG - chr5.3:6371336-6371355 None:intergenic 55.0%
! GGATTCTGCATCTGAGGTGG+AGG + chr5.3:6371307-6371326 MS.gene09720:CDS 55.0%
!! ACGAGAAGCGATGGTGGAAG+CGG - chr5.3:6371099-6371118 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 6370897 6371439 6370897 ID=MS.gene09720
chr5.3 mRNA 6370897 6371439 6370897 ID=MS.gene09720.t1;Parent=MS.gene09720
chr5.3 exon 6370897 6370920 6370897 ID=MS.gene09720.t1.exon1;Parent=MS.gene09720.t1
chr5.3 CDS 6370897 6370920 6370897 ID=cds.MS.gene09720.t1;Parent=MS.gene09720.t1
chr5.3 exon 6371038 6371439 6371038 ID=MS.gene09720.t1.exon2;Parent=MS.gene09720.t1
chr5.3 CDS 6371038 6371439 6371038 ID=cds.MS.gene09720.t1;Parent=MS.gene09720.t1
Gene Sequence

>MS.gene09720

ATGGCTTCCCAAGTATTTCTTCAGGTTTTTTTTTTTTCTTTCCCTCTCATCTCATTATTATCCAATTCATTGTAACAAATACTTATGATGCATTTTCAATTTCAATTTCACTACTTAACTCAAAAACTTTCATTTTAACAGACACTATTTGCTCCTAATCAATGTCCTCCAACAAAGCTAGGTTTCTCTTCTGCTTGCTCCGCTTCCACCATCGCTTCTCGTAACTTACCCTTCATTTCCAGGGCTTCTTCGACCTCAATTTCATGGCGCTGTAATCCTCCTCTCATTGCTAAACCTAGTTTTATTGTGAGAGCTGATTCCAATTCCGAGGCTGTTTCAAATGTTGATGAAAATCCTGAAGCTTCGGATAATGCTGATGAAGTTTCGGAAACTGAAGGAGAACAGGTTTTGGATTCTGCATCTGAGGTGGAGGAAGCCAAGCCGCCTAGACAAACTCGTGTTAAGCTTGGTGATGTTATGGGGGTAAGTTATTTCTTCCATGACTCTATACTGTTATTAATGATTAAGTTGAAATTTGAGTAA

Protein sequence

>MS.gene09720.t1

MASQVFLQTLFAPNQCPPTKLGFSSACSASTIASRNLPFISRASSTSISWRCNPPLIAKPSFIVRADSNSEAVSNVDENPEASDNADEVSETEGEQVLDSASEVEEAKPPRQTRVKLGDVMGVSYFFHDSILLLMIKLKFE