AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52289


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52289.t1 MTR_7g084800 100.000 161 0 0 1 161 244 404 1.53e-113 328
MS.gene52289.t1 MTR_3g115920 85.714 161 22 1 1 160 128 288 3.44e-79 237
MS.gene52289.t1 MTR_3g115920 85.714 161 22 1 1 160 262 422 5.18e-78 239
MS.gene52289.t1 MTR_3g115920 86.301 146 19 1 1 145 262 407 5.83e-69 214
MS.gene52289.t1 MTR_3g085850 49.020 153 77 1 1 152 135 287 3.36e-46 152
MS.gene52289.t1 MTR_3g085850 49.020 153 77 1 1 152 180 332 1.03e-45 152
MS.gene52289.t1 MTR_2g065470 49.673 153 76 1 1 152 196 348 4.03e-43 146
MS.gene52289.t1 MTR_4g103920 48.366 153 78 1 1 152 179 331 8.47e-43 145
MS.gene52289.t1 MTR_2g065470 49.673 153 76 1 1 152 255 407 1.07e-42 146
MS.gene52289.t1 MTR_1680s0010 54.545 132 60 0 1 132 1 132 6.86e-40 138
MS.gene52289.t1 MTR_2g065470 48.630 146 74 1 1 145 255 400 4.01e-39 137
MS.gene52289.t1 MTR_6g022630 45.890 146 78 1 1 145 177 322 7.38e-39 134
MS.gene52289.t1 MTR_8g046560 42.466 146 82 2 1 145 114 258 6.62e-30 114
MS.gene52289.t1 MTR_8g464820 43.836 146 72 3 1 145 1 137 1.31e-29 111
MS.gene52289.t1 MTR_4g103920 52.809 89 41 1 1 88 179 267 7.38e-23 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52289.t1 AT1G12900 95.031 161 8 0 1 161 157 317 7.85e-93 272
MS.gene52289.t1 AT1G12900 95.031 161 8 0 1 161 190 350 3.03e-91 269
MS.gene52289.t1 AT1G12900 95.031 161 8 0 1 161 190 350 3.03e-91 269
MS.gene52289.t1 AT1G12900 95.031 161 8 0 1 161 239 399 4.56e-91 270
MS.gene52289.t1 AT3G26650 94.410 161 9 0 1 161 236 396 9.96e-91 270
MS.gene52289.t1 AT1G12900 95.031 161 8 0 1 161 281 441 1.04e-90 271
MS.gene52289.t1 AT1G42970 83.851 161 25 1 1 160 258 418 6.07e-84 254
MS.gene52289.t1 AT1G13440 49.020 153 77 1 1 152 151 303 4.40e-46 152
MS.gene52289.t1 AT1G13440 49.020 153 77 1 1 152 179 331 1.42e-45 152
MS.gene52289.t1 AT3G04120 49.020 153 77 1 1 152 179 331 1.86e-45 152
MS.gene52289.t1 AT1G16300 47.468 158 82 1 1 157 257 414 1.95e-42 146
MS.gene52289.t1 AT1G79530 48.101 158 81 1 1 157 259 416 2.07e-42 146

Find 47 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGCTCTCACTTCCAGTTAT+TGG 0.185348 7.3:+23850023 None:intergenic
CTCACAAAGGGTTGTTGATT+TGG 0.245442 7.3:-23850058 MS.gene52289:CDS
CTGACATTGTTGCCAATAAC+TGG 0.306527 7.3:-23850035 MS.gene52289:CDS
CTTGTCCTCCCATCACTTAA+AGG 0.338998 7.3:-23850363 MS.gene52289:CDS
GGCCACTGCCTTTGCTGCTC+CGG 0.348204 7.3:+23850386 None:intergenic
ATGCTAGCCACCGTGATCTA+AGG 0.366376 7.3:-23850449 MS.gene52289:CDS
AACATTGTGCCAACTTCAAC+CGG 0.389585 7.3:-23850405 MS.gene52289:CDS
TGCTTGGTATGATAATGAAT+GGG 0.460745 7.3:-23850085 MS.gene52289:CDS
AAGCCTTTGGTCACCGGTGT+AGG 0.479065 7.3:+23850476 None:intergenic
GGCTAGCATCAAGAAGCCTT+TGG 0.481799 7.3:+23850463 None:intergenic
AGGTCAACAACCGAGACGTT+TGG 0.488985 7.3:+23850306 None:intergenic
TTTGCTGCTCCGGTTGAAGT+TGG 0.491416 7.3:+23850396 None:intergenic
TTGAGCTTGCCTTTAAGTGA+TGG 0.493574 7.3:+23850354 None:intergenic
TGAGCTTGCCTTTAAGTGAT+GGG 0.495886 7.3:+23850355 None:intergenic
TTTCAGAGACAGTGCAGCCA+AGG 0.512789 7.3:-23850235 MS.gene52289:CDS
TTCGTCATTGACAATGGTCA+TGG 0.513254 7.3:-23850130 MS.gene52289:CDS
ATCAAGAAGCCTTTGGTCAC+CGG 0.515096 7.3:+23850470 None:intergenic
GAGAATACCGCTCAGCTCCT+TGG 0.524534 7.3:+23850218 None:intergenic
CTTTAAGTGATGGGAGGACA+AGG 0.529210 7.3:+23850364 None:intergenic
AACTTCAACCGGAGCAGCAA+AGG 0.533569 7.3:-23850394 MS.gene52289:CDS
TTGCTTGGTATGATAATGAA+TGG 0.536174 7.3:-23850086 MS.gene52289:CDS
AACCGGAGCAGCAAAGGCAG+TGG 0.541553 7.3:-23850388 MS.gene52289:CDS
TGAATGGGGTTACTCACAAA+GGG 0.544864 7.3:-23850070 MS.gene52289:CDS
CTCTTGCACGCCTTAGATCA+CGG 0.552003 7.3:+23850439 None:intergenic
ATTTGCTGAAGAAGTGAATG+AGG 0.560532 7.3:-23850259 MS.gene52289:CDS
CGTTGATTCGTCATTGACAA+TGG 0.579256 7.3:-23850136 MS.gene52289:CDS
ATGAATGGGGTTACTCACAA+AGG 0.581529 7.3:-23850071 MS.gene52289:CDS
CAATGACGAATCAACGGTTG+AGG 0.591375 7.3:+23850143 None:intergenic
ACATGGTGAAGGTGATTGCT+TGG 0.591983 7.3:-23850101 MS.gene52289:CDS
ACTACCACTCACTCCTACAC+CGG 0.595936 7.3:-23850489 MS.gene52289:CDS
ACAACCGAGACGTTTGGTGT+TGG 0.599911 7.3:+23850312 None:intergenic
TCGTCATTGACAATGGTCAT+GGG 0.600931 7.3:-23850129 MS.gene52289:CDS
ACTCCTACACCGGTGACCAA+AGG 0.601118 7.3:-23850479 MS.gene52289:CDS
GTCACCGGTGTAGGAGTGAG+TGG 0.617969 7.3:+23850485 None:intergenic
TGTGCCAACACCAAACGTCT+CGG 0.622747 7.3:-23850316 MS.gene52289:CDS
TCACTTAAAGGCAAGCTCAA+CGG 0.626795 7.3:-23850351 MS.gene52289:CDS
CATTGTCAATGACGAATCAA+CGG 0.634207 7.3:+23850137 None:intergenic
CTAAAATCTACAGACACAAG+TGG 0.644572 7.3:+23850180 None:intergenic
GCTTGGTATGATAATGAATG+GGG 0.645070 7.3:-23850084 MS.gene52289:CDS
GCTTGCCTTTAAGTGATGGG+AGG 0.645422 7.3:+23850358 None:intergenic
GTAGGAGTGAGTGGTAGTCA+TGG 0.651863 7.3:+23850494 None:intergenic
AATGGTCATGGGAGATGACA+TGG 0.657489 7.3:-23850118 MS.gene52289:CDS
CATGGGAGATGACATGGTGA+AGG 0.682296 7.3:-23850112 MS.gene52289:CDS
TTTAAGTGATGGGAGGACAA+GGG 0.684817 7.3:+23850365 None:intergenic
AGTGCAGCCAAGGAGCTGAG+CGG 0.723974 7.3:-23850225 MS.gene52289:CDS
TTGCACGCCTTAGATCACGG+TGG 0.804209 7.3:+23850442 None:intergenic
TCTTTGAGACTTGAACAACG+AGG 0.832544 7.3:+23850286 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGCTTGGTATGATAATGAAT+GGG - chr7.3:23850436-23850455 MS.gene52289:CDS 30.0%
! TTGCTTGGTATGATAATGAA+TGG - chr7.3:23850435-23850454 MS.gene52289:CDS 30.0%
CATTGTCAATGACGAATCAA+CGG + chr7.3:23850387-23850406 None:intergenic 35.0%
CTAAAATCTACAGACACAAG+TGG + chr7.3:23850344-23850363 None:intergenic 35.0%
! ATTTGCTGAAGAAGTGAATG+AGG - chr7.3:23850262-23850281 MS.gene52289:CDS 35.0%
! CACTTGTGTCTGTAGATTTT+AGG - chr7.3:23850342-23850361 MS.gene52289:CDS 35.0%
! GCTTGGTATGATAATGAATG+GGG - chr7.3:23850437-23850456 MS.gene52289:CDS 35.0%
AACATTGTGCCAACTTCAAC+CGG - chr7.3:23850116-23850135 MS.gene52289:CDS 40.0%
ATGAATGGGGTTACTCACAA+AGG - chr7.3:23850450-23850469 MS.gene52289:CDS 40.0%
CTGACATTGTTGCCAATAAC+TGG - chr7.3:23850486-23850505 MS.gene52289:CDS 40.0%
TCACTTAAAGGCAAGCTCAA+CGG - chr7.3:23850170-23850189 MS.gene52289:CDS 40.0%
TCGTCATTGACAATGGTCAT+GGG - chr7.3:23850392-23850411 MS.gene52289:CDS 40.0%
TGAATGGGGTTACTCACAAA+GGG - chr7.3:23850451-23850470 MS.gene52289:CDS 40.0%
TGAGCTTGCCTTTAAGTGAT+GGG + chr7.3:23850169-23850188 None:intergenic 40.0%
TTCGTCATTGACAATGGTCA+TGG - chr7.3:23850391-23850410 MS.gene52289:CDS 40.0%
TTGAGCTTGCCTTTAAGTGA+TGG + chr7.3:23850170-23850189 None:intergenic 40.0%
! CTCACAAAGGGTTGTTGATT+TGG - chr7.3:23850463-23850482 MS.gene52289:CDS 40.0%
! TTTAAGTGATGGGAGGACAA+GGG + chr7.3:23850159-23850178 None:intergenic 40.0%
!! CGTTGATTCGTCATTGACAA+TGG - chr7.3:23850385-23850404 MS.gene52289:CDS 40.0%
!! TCTTTGAGACTTGAACAACG+AGG + chr7.3:23850238-23850257 None:intergenic 40.0%
AATGGTCATGGGAGATGACA+TGG - chr7.3:23850403-23850422 MS.gene52289:CDS 45.0%
ACATGGTGAAGGTGATTGCT+TGG - chr7.3:23850420-23850439 MS.gene52289:CDS 45.0%
ATCAAGAAGCCTTTGGTCAC+CGG + chr7.3:23850054-23850073 None:intergenic 45.0%
CAATGACGAATCAACGGTTG+AGG + chr7.3:23850381-23850400 None:intergenic 45.0%
CTTGTCCTCCCATCACTTAA+AGG - chr7.3:23850158-23850177 MS.gene52289:CDS 45.0%
! CTTTAAGTGATGGGAGGACA+AGG + chr7.3:23850160-23850179 None:intergenic 45.0%
AACTTCAACCGGAGCAGCAA+AGG - chr7.3:23850127-23850146 MS.gene52289:CDS 50.0%
ACTACCACTCACTCCTACAC+CGG - chr7.3:23850032-23850051 MS.gene52289:CDS 50.0%
AGGTCAACAACCGAGACGTT+TGG + chr7.3:23850218-23850237 None:intergenic 50.0%
ATGCTAGCCACCGTGATCTA+AGG - chr7.3:23850072-23850091 MS.gene52289:CDS 50.0%
CATGGGAGATGACATGGTGA+AGG - chr7.3:23850409-23850428 MS.gene52289:CDS 50.0%
CTCTTGCACGCCTTAGATCA+CGG + chr7.3:23850085-23850104 None:intergenic 50.0%
GGCTAGCATCAAGAAGCCTT+TGG + chr7.3:23850061-23850080 None:intergenic 50.0%
TGTGCCAACACCAAACGTCT+CGG - chr7.3:23850205-23850224 MS.gene52289:CDS 50.0%
TTTCAGAGACAGTGCAGCCA+AGG - chr7.3:23850286-23850305 MS.gene52289:CDS 50.0%
TTTGCTGCTCCGGTTGAAGT+TGG + chr7.3:23850128-23850147 None:intergenic 50.0%
! GCTTGCCTTTAAGTGATGGG+AGG + chr7.3:23850166-23850185 None:intergenic 50.0%
!! ACAACCGAGACGTTTGGTGT+TGG + chr7.3:23850212-23850231 None:intergenic 50.0%
AAGCCTTTGGTCACCGGTGT+AGG + chr7.3:23850048-23850067 None:intergenic 55.0%
ACTCCTACACCGGTGACCAA+AGG - chr7.3:23850042-23850061 MS.gene52289:CDS 55.0%
GAGAATACCGCTCAGCTCCT+TGG + chr7.3:23850306-23850325 None:intergenic 55.0%
TTGCACGCCTTAGATCACGG+TGG + chr7.3:23850082-23850101 None:intergenic 55.0%
AACCGGAGCAGCAAAGGCAG+TGG - chr7.3:23850133-23850152 MS.gene52289:CDS 60.0%
AGTGCAGCCAAGGAGCTGAG+CGG - chr7.3:23850296-23850315 MS.gene52289:CDS 60.0%
GTCACCGGTGTAGGAGTGAG+TGG + chr7.3:23850039-23850058 None:intergenic 60.0%
GGCCACTGCCTTTGCTGCTC+CGG + chr7.3:23850138-23850157 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 23850029 23850514 23850029 ID=MS.gene52289
chr7.3 mRNA 23850029 23850514 23850029 ID=MS.gene52289.t1;Parent=MS.gene52289
chr7.3 exon 23850029 23850514 23850029 ID=MS.gene52289.t1.exon1;Parent=MS.gene52289.t1
chr7.3 CDS 23850029 23850514 23850029 ID=cds.MS.gene52289.t1;Parent=MS.gene52289.t1
Gene Sequence

>MS.gene52289

ATGACTACCACTCACTCCTACACCGGTGACCAAAGGCTTCTTGATGCTAGCCACCGTGATCTAAGGCGTGCAAGAGCAGCAGCTCTTAACATTGTGCCAACTTCAACCGGAGCAGCAAAGGCAGTGGCCCTTGTCCTCCCATCACTTAAAGGCAAGCTCAACGGTATTGCACTTCGTGTGCCAACACCAAACGTCTCGGTTGTTGACCTCGTTGTTCAAGTCTCAAAGAAGACATTTGCTGAAGAAGTGAATGAGGCTTTCAGAGACAGTGCAGCCAAGGAGCTGAGCGGTATTCTCTCAGTTTGTGATGAGCCACTTGTGTCTGTAGATTTTAGGTGCACTGATGTGTCCTCAACCGTTGATTCGTCATTGACAATGGTCATGGGAGATGACATGGTGAAGGTGATTGCTTGGTATGATAATGAATGGGGTTACTCACAAAGGGTTGTTGATTTGGCTGACATTGTTGCCAATAACTGGAAGTGA

Protein sequence

>MS.gene52289.t1

MTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLPSLKGKLNGIALRVPTPNVSVVDLVVQVSKKTFAEEVNEAFRDSAAKELSGILSVCDEPLVSVDFRCTDVSSTVDSSLTMVMGDDMVKVIAWYDNEWGYSQRVVDLADIVANNWK