AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23996


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23996.t1 MTR_1g069465 100.000 182 0 0 1 182 1 182 1.80e-136 378
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23996.t1 AT4G28660 68.966 145 45 0 37 181 37 181 1.03e-71 215
MS.gene23996.t1 AT4G28660 68.966 145 45 0 37 181 52 196 2.52e-71 214

Find 42 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTGAATGAATTGAATTT+TGG 0.043355 1.3:+48909711 None:intergenic
GGAGAAGTAGGCGACATTAC+TGG 0.365446 1.3:-48909583 MS.gene23996:CDS
CCCAAAATTCAATTCATTCA+AGG 0.375868 1.3:-48909712 MS.gene23996:CDS
AACTTAAATTCCTCATTCAA+TGG 0.412356 1.3:-48909823 MS.gene23996:CDS
TTCCCCGCCATCATGATAAC+CGG 0.429760 1.3:+48909736 None:intergenic
TGGAGTCCGCATTATATACT+TGG 0.434350 1.3:+48909474 None:intergenic
CAAATGGCATGGCTATCTTC+AGG 0.434591 1.3:-48909636 MS.gene23996:CDS
ATTTGCATGTGCCAAGTTTG+AGG 0.436765 1.3:-48909795 MS.gene23996:CDS
TAATGCGGACTCCACGGGAC+TGG 0.442232 1.3:-48909465 MS.gene23996:CDS
AAATCAAAGGATGGAACAAA+TGG 0.447952 1.3:-48909652 MS.gene23996:CDS
TTTGATTCTTCTGGAGAAGT+AGG 0.453284 1.3:-48909595 MS.gene23996:CDS
GGATAGGTTCATGAGATTCA+TGG 0.460311 1.3:-48909443 MS.gene23996:CDS
CATTTACATCAACAGATTGA+AGG 0.465513 1.3:+48909533 None:intergenic
GCCACTTCTTTAATTATCCA+TGG 0.476745 1.3:-48909853 MS.gene23996:CDS
GAGCGATACTCTGATGCTAA+TGG 0.480616 1.3:-48909421 MS.gene23996:CDS
AATTTAAGTTCAAACTTCCA+TGG 0.492114 1.3:+48909836 None:intergenic
CATGATAACCGGCATGTGCA+TGG 0.501061 1.3:+48909747 None:intergenic
ATGCCGGTTATCATGATGGC+GGG 0.504435 1.3:-48909739 MS.gene23996:CDS
CAGAAGCCACGCTCATACTC+AGG 0.512959 1.3:-48910590 MS.gene23996:intron
AATGCGGACTCCACGGGACT+GGG 0.524053 1.3:-48909464 MS.gene23996:CDS
GGACTCCACGGGACTGGGAT+AGG 0.532501 1.3:-48909459 MS.gene23996:CDS
GTATATAATGCGGACTCCAC+GGG 0.541871 1.3:-48909470 MS.gene23996:CDS
GCACATGCCGGTTATCATGA+TGG 0.545209 1.3:-48909743 MS.gene23996:CDS
ATACTTGGCTTCAATTCCTG+AGG 0.546032 1.3:+48909489 None:intergenic
ATCATCGGTAACCTCAAACT+TGG 0.546137 1.3:+48909784 None:intergenic
ATGATAACCGGCATGTGCAT+GGG 0.547496 1.3:+48909748 None:intergenic
AATTGAGTTGTAGAGTGGAG+AGG 0.552148 1.3:+48910613 None:intergenic
TCCATGGATAATTAAAGAAG+TGG 0.555425 1.3:+48909852 None:intergenic
TTTGTCAACGGAAAGCCCTC+AGG 0.559746 1.3:-48909505 MS.gene23996:CDS
AGTATATAATGCGGACTCCA+CGG 0.565595 1.3:-48909471 MS.gene23996:CDS
TACTTGGCTTCAATTCCTGA+GGG 0.571164 1.3:+48909490 None:intergenic
AAGCTGACAAAATCAAAGGA+TGG 0.597295 1.3:-48909661 MS.gene23996:CDS
GTAAATGCTAAATTTGTCAA+CGG 0.597700 1.3:-48909517 MS.gene23996:CDS
CAAATGTTGACCATTGAATG+AGG 0.613868 1.3:+48909813 None:intergenic
TGCCGGTTATCATGATGGCG+GGG 0.623175 1.3:-48909738 MS.gene23996:CDS
AAAGGATGGAACAAATGGCA+TGG 0.632758 1.3:-48909647 MS.gene23996:CDS
CATGCCGGTTATCATGATGG+CGG 0.658092 1.3:-48909740 MS.gene23996:CDS
TGTGAAGCTGACAAAATCAA+AGG 0.662632 1.3:-48909665 MS.gene23996:CDS
TTCTGAATTGAGTTGTAGAG+TGG 0.666148 1.3:+48910608 None:intergenic
ATGAACCTATCCCAGTCCCG+TGG 0.667895 1.3:+48909454 None:intergenic
TTTGTACCTGAGTATGAGCG+TGG 0.677678 1.3:+48910584 None:intergenic
TTGAAGCCAAGTATATAATG+CGG 0.690031 1.3:-48909480 MS.gene23996:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAACAAAACAAAAGAT+GGG - chr1.3:48909702-48909721 MS.gene23996:CDS 15.0%
!! CAAATAAACAAAACAAAAGA+TGG - chr1.3:48909701-48909720 MS.gene23996:CDS 20.0%
!! GTAAATTCATAATATGAATC+AGG - chr1.3:48909601-48909620 MS.gene23996:CDS 20.0%
!! TTAATTTACAAGTCAACAAT+TGG + chr1.3:48909664-48909683 None:intergenic 20.0%
!!! CATCTTTTGTTTTGTTTATT+TGG + chr1.3:48909703-48909722 None:intergenic 20.0%
! AACTTAAATTCCTCATTCAA+TGG - chr1.3:48910217-48910236 MS.gene23996:intron 25.0%
! AGAGAATACTTGAAACTAAT+GGG + chr1.3:48910004-48910023 None:intergenic 25.0%
! GTAAATGCTAAATTTGTCAA+CGG - chr1.3:48910523-48910542 MS.gene23996:intron 25.0%
! TAGAGAATACTTGAAACTAA+TGG + chr1.3:48910005-48910024 None:intergenic 25.0%
! TGTTGTCAAGAAAAAAATGT+TGG + chr1.3:48909758-48909777 None:intergenic 25.0%
! TTATACTTGTACTAGAATTG+TGG - chr1.3:48909897-48909916 MS.gene23996:intron 25.0%
!! AATTTAAGTTCAAACTTCCA+TGG + chr1.3:48910207-48910226 None:intergenic 25.0%
!! ATGAATCAGGTTAACATTTT+TGG - chr1.3:48909614-48909633 MS.gene23996:CDS 25.0%
!! CCTTGAATGAATTGAATTTT+GGG + chr1.3:48910331-48910350 None:intergenic 25.0%
!! TCCTTGAATGAATTGAATTT+TGG + chr1.3:48910332-48910351 None:intergenic 25.0%
!! TGAATCAGGTTAACATTTTT+GGG - chr1.3:48909615-48909634 MS.gene23996:CDS 25.0%
!! TGGTTTTTATATGATTGATG+AGG - chr1.3:48910477-48910496 MS.gene23996:intron 25.0%
!! TTTTATATGATTGATGAGGA+AGG - chr1.3:48910481-48910500 MS.gene23996:intron 25.0%
!!! ATGTTTTGAGAACTTATGTT+TGG - chr1.3:48909533-48909552 MS.gene23996:CDS 25.0%
!!! CTTTGTTTGTTATTGTTTTG+TGG - chr1.3:48909500-48909519 MS.gene23996:CDS 25.0%
!!! GCATCACTTTTTATTTTCTA+TGG + chr1.3:48910117-48910136 None:intergenic 25.0%
AAATCAAAGGATGGAACAAA+TGG - chr1.3:48910388-48910407 MS.gene23996:intron 30.0%
AAGTTCTCAAAACATTTCAC+AGG + chr1.3:48909528-48909547 None:intergenic 30.0%
ATCATGTGTTCAAGATTTCA+AGG + chr1.3:48910076-48910095 None:intergenic 30.0%
CAATTGGAGCAAACTATATA+AGG + chr1.3:48909648-48909667 None:intergenic 30.0%
CATTTACATCAACAGATTGA+AGG + chr1.3:48910510-48910529 None:intergenic 30.0%
CCCAAAATTCAATTCATTCA+AGG - chr1.3:48910328-48910347 MS.gene23996:intron 30.0%
TCCATGGATAATTAAAGAAG+TGG + chr1.3:48910191-48910210 None:intergenic 30.0%
TTCTTGCATTGTTCAGTATT+TGG - chr1.3:48909782-48909801 MS.gene23996:CDS 30.0%
TTGAAGCCAAGTATATAATG+CGG - chr1.3:48910560-48910579 MS.gene23996:intron 30.0%
! GAATCAGGTTAACATTTTTG+GGG - chr1.3:48909616-48909635 MS.gene23996:CDS 30.0%
!!! GGAGTTTTTTGAGTAATCAT+CGG + chr1.3:48910274-48910293 None:intergenic 30.0%
!!! GTTTTGTTTATTTGGCATTC+TGG + chr1.3:48909695-48909714 None:intergenic 30.0%
AAGCTGACAAAATCAAAGGA+TGG - chr1.3:48910379-48910398 MS.gene23996:intron 35.0%
AGAACATCATACATACCATC+AGG + chr1.3:48909834-48909853 None:intergenic 35.0%
CAAATGTTGACCATTGAATG+AGG + chr1.3:48910230-48910249 None:intergenic 35.0%
CCAGAAGAATCAAAAACTGA+GGG + chr1.3:48910439-48910458 None:intergenic 35.0%
GAACATCATACATACCATCA+GGG + chr1.3:48909833-48909852 None:intergenic 35.0%
GATGGTATGTATGATGTTCT+TGG - chr1.3:48909834-48909853 MS.gene23996:CDS 35.0%
GCCACTTCTTTAATTATCCA+TGG - chr1.3:48910187-48910206 MS.gene23996:intron 35.0%
TACTAGAATTGTGGAGTAAC+AGG - chr1.3:48909906-48909925 MS.gene23996:intron 35.0%
TCCAGAAGAATCAAAAACTG+AGG + chr1.3:48910440-48910459 None:intergenic 35.0%
TGTGAAGCTGACAAAATCAA+AGG - chr1.3:48910375-48910394 MS.gene23996:intron 35.0%
TGTTCAAGATTTCAAGGTCA+GGG + chr1.3:48910070-48910089 None:intergenic 35.0%
! TTCTGAATTGAGTTGTAGAG+TGG + chr1.3:48909435-48909454 None:intergenic 35.0%
! TTTGATTCTTCTGGAGAAGT+AGG - chr1.3:48910445-48910464 MS.gene23996:intron 35.0%
!!! GGTTAACATTTTTGGGGTTA+AGG - chr1.3:48909622-48909641 MS.gene23996:CDS 35.0%
AAAGGATGGAACAAATGGCA+TGG - chr1.3:48910393-48910412 MS.gene23996:intron 40.0%
AGTATATAATGCGGACTCCA+CGG - chr1.3:48910569-48910588 MS.gene23996:intron 40.0%
ATACTTGGCTTCAATTCCTG+AGG + chr1.3:48910554-48910573 None:intergenic 40.0%
ATCATCGGTAACCTCAAACT+TGG + chr1.3:48910259-48910278 None:intergenic 40.0%
ATGTTCTTGGCAACACTCTA+TGG - chr1.3:48909847-48909866 MS.gene23996:CDS 40.0%
ATTTGCATGTGCCAAGTTTG+AGG - chr1.3:48910245-48910264 MS.gene23996:intron 40.0%
CAACACTCTATGGAACAGAA+TGG - chr1.3:48909857-48909876 MS.gene23996:intron 40.0%
CTATGGAACAGAATGGCATT+GGG - chr1.3:48909864-48909883 MS.gene23996:intron 40.0%
GGATAGGTTCATGAGATTCA+TGG - chr1.3:48910597-48910616 MS.gene23996:CDS 40.0%
GTGTTCAAGATTTCAAGGTC+AGG + chr1.3:48910071-48910090 None:intergenic 40.0%
GTTCAAGATTTCAAGGTCAG+GGG + chr1.3:48910069-48910088 None:intergenic 40.0%
TAATATAGCTTCCACCCTGA+TGG - chr1.3:48909816-48909835 MS.gene23996:CDS 40.0%
TACTTGGCTTCAATTCCTGA+GGG + chr1.3:48910553-48910572 None:intergenic 40.0%
TCTATGGAACAGAATGGCAT+TGG - chr1.3:48909863-48909882 MS.gene23996:intron 40.0%
TGGAGTCCGCATTATATACT+TGG + chr1.3:48910569-48910588 None:intergenic 40.0%
! AATTGAGTTGTAGAGTGGAG+AGG + chr1.3:48909430-48909449 None:intergenic 40.0%
! GATTTTGTCAGCTTCACATC+AGG + chr1.3:48910373-48910392 None:intergenic 40.0%
!! CCCTCAGTTTTTGATTCTTC+TGG - chr1.3:48910436-48910455 MS.gene23996:intron 40.0%
AAAAACTCCCATGCACATGC+CGG - chr1.3:48910285-48910304 MS.gene23996:intron 45.0%
ATGATAACCGGCATGTGCAT+GGG + chr1.3:48910295-48910314 None:intergenic 45.0%
ATTTCAAGGTCAGGGGTCAA+GGG + chr1.3:48910062-48910081 None:intergenic 45.0%
CAAATGGCATGGCTATCTTC+AGG - chr1.3:48910404-48910423 MS.gene23996:intron 45.0%
CATCATACATACCATCAGGG+TGG + chr1.3:48909830-48909849 None:intergenic 45.0%
GAGCGATACTCTGATGCTAA+TGG - chr1.3:48910619-48910638 MS.gene23996:CDS 45.0%
GTATATAATGCGGACTCCAC+GGG - chr1.3:48910570-48910589 MS.gene23996:intron 45.0%
TTTGTACCTGAGTATGAGCG+TGG + chr1.3:48909459-48909478 None:intergenic 45.0%
CATGATAACCGGCATGTGCA+TGG + chr1.3:48910296-48910315 None:intergenic 50.0%
GATTTCAAGGTCAGGGGTCA+AGG + chr1.3:48910063-48910082 None:intergenic 50.0%
GGAGAAGTAGGCGACATTAC+TGG - chr1.3:48910457-48910476 MS.gene23996:intron 50.0%
TTCCCCGCCATCATGATAAC+CGG + chr1.3:48910307-48910326 None:intergenic 50.0%
TTTGTCAACGGAAAGCCCTC+AGG - chr1.3:48910535-48910554 MS.gene23996:intron 50.0%
! ATGCCGGTTATCATGATGGC+GGG - chr1.3:48910301-48910320 MS.gene23996:intron 50.0%
! CATGCCGGTTATCATGATGG+CGG - chr1.3:48910300-48910319 MS.gene23996:intron 50.0%
! GCACATGCCGGTTATCATGA+TGG - chr1.3:48910297-48910316 MS.gene23996:intron 50.0%
ATGAACCTATCCCAGTCCCG+TGG + chr1.3:48910589-48910608 None:intergenic 55.0%
CAGAAGCCACGCTCATACTC+AGG - chr1.3:48909450-48909469 MS.gene23996:CDS 55.0%
! TGCCGGTTATCATGATGGCG+GGG - chr1.3:48910302-48910321 MS.gene23996:intron 55.0%
AATGCGGACTCCACGGGACT+GGG - chr1.3:48910576-48910595 MS.gene23996:intron 60.0%
TAATGCGGACTCCACGGGAC+TGG - chr1.3:48910575-48910594 MS.gene23996:intron 60.0%
GGACTCCACGGGACTGGGAT+AGG - chr1.3:48910581-48910600 MS.gene23996:intron 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 48909399 48910663 48909399 ID=MS.gene23996
chr1.3 mRNA 48909399 48910663 48909399 ID=MS.gene23996.t1;Parent=MS.gene23996
chr1.3 exon 48910591 48910663 48910591 ID=MS.gene23996.t1.exon1;Parent=MS.gene23996.t1
chr1.3 CDS 48910591 48910663 48910591 ID=cds.MS.gene23996.t1;Parent=MS.gene23996.t1
chr1.3 exon 48909399 48909874 48909399 ID=MS.gene23996.t1.exon2;Parent=MS.gene23996.t1
chr1.3 CDS 48909399 48909874 48909399 ID=cds.MS.gene23996.t1;Parent=MS.gene23996.t1
Gene Sequence

>MS.gene23996

ATGGCAACTATGCACTCTCTTGCATTCTCCTCTCCACTCTACAACTCAATTCAGAAGCCACGCTCATACTCAGGTACAAACACTCTCTTCAACATTACTTTCTTTGTTTGTTATTGTTTTGTGGTTCCTGTGAAATGTTTTGAGAACTTATGTTTGGACTATTCAATTTTATAACAGTTGTCAGATTTTTAAAAAAATAACAGTAAATTCATAATATGAATCAGGTTAACATTTTTGGGGTTAAGGCCTTATATAGTTTGCTCCAATTGTTGACTTGTAAATTAATGTGCTTTCCAGAATGCCAAATAAACAAAACAAAAGATGGGTGACATTATTTTCTTTTGTTTATCTTTTTGCCAACATTTTTTTCTTGACAACATTAATTCTTGCATTGTTCAGTATTTGGAAACAATAATTTAATATAGCTTCCACCCTGATGGTATGTATGATGTTCTTGGCAACACTCTATGGAACAGAATGGCATTGGGAAGTTTTGAGTTATACTTGTACTAGAATTGTGGAGTAACAGGACAATAGAAAATTTGCAATTAATAACAAAATTGTACTTTTGCAATGTATGAACATGAAGTAATCTTAGAATTCCCATTAGTTTCAAGTATTCTCTATACATGTAGAGCAATTTCTAGTATATCATTGCTTCCCTTGACCCCTGACCTTGAAATCTTGAACACATGATTTCTTTGAAGATTTTTAGCCATAGAAAATAAAAAGTGATGCATATGATATATGATATGCATGATGAAAATTATAGACTATTTGTTTATGCAGCCACTTCTTTAATTATCCATGGAAGTTTGAACTTAAATTCCTCATTCAATGGTCAACATTTGCATGTGCCAAGTTTGAGGTTACCGATGATTACTCAAAAAACTCCCATGCACATGCCGGTTATCATGATGGCGGGGAAGCCCAAAATTCAATTCATTCAAGGAACTGATGAACTGACAATACCTGATGTGAAGCTGACAAAATCAAAGGATGGAACAAATGGCATGGCTATCTTCAGGTTTGATCAACCCTCAGTTTTTGATTCTTCTGGAGAAGTAGGCGACATTACTGGTTTTTATATGATTGATGAGGAAGGAGTCCTTCAATCTGTTGATGTAAATGCTAAATTTGTCAACGGAAAGCCCTCAGGAATTGAAGCCAAGTATATAATGCGGACTCCACGGGACTGGGATAGGTTCATGAGATTCATGGAGCGATACTCTGATGCTAATGGTTTGCAATTCATAAAATATTGA

Protein sequence

>MS.gene23996.t1

MATMHSLAFSSPLYNSIQKPRSYSATSLIIHGSLNLNSSFNGQHLHVPSLRLPMITQKTPMHMPVIMMAGKPKIQFIQGTDELTIPDVKLTKSKDGTNGMAIFRFDQPSVFDSSGEVGDITGFYMIDEEGVLQSVDVNAKFVNGKPSGIEAKYIMRTPRDWDRFMRFMERYSDANGLQFIKY