AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73381


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73381.t1 MTR_7g084160 96.970 198 6 0 1 198 1 198 1.82e-142 397
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73381.t1 AT3G26710 82.482 137 23 1 63 198 68 204 3.13e-76 230

Find 55 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACGAAGTTTCTTTGGTTT+TGG 0.222781 7.2:-23363101 None:intergenic
TTATCTCTGTTCCTGGTCTT+TGG 0.246420 7.2:+23363255 MS.gene73381:CDS
TGCCGGATGTTGGCAATAAT+TGG 0.247297 7.2:+23364246 MS.gene73381:CDS
TATAGATGAAGGTGGAAATA+AGG 0.285189 7.2:+23363404 MS.gene73381:CDS
AGGGATAAACGAAGTTTCTT+TGG 0.332452 7.2:-23363107 None:intergenic
AGAGACTACTGGTTACTCTT+TGG 0.348247 7.2:+23363202 MS.gene73381:CDS
TGTAAGTAACAAGAGAGAAT+TGG 0.349828 7.2:-23363180 None:intergenic
GCTAGTTATTATACTTCTCT+TGG 0.366409 7.2:+23363224 MS.gene73381:CDS
GCCAACATCCGGCACGGTTA+TGG 0.378017 7.2:-23364237 None:intergenic
ATGTTGGCAATAATTGGTTT+TGG 0.387394 7.2:+23364252 MS.gene73381:CDS
AGAGGGAGATGGTGTGGATG+TGG 0.397897 7.2:-23363017 None:intergenic
GTAAGTAACAAGAGAGAATT+GGG 0.407638 7.2:-23363179 None:intergenic
TTTCTTCTATGAAGGTTTGA+AGG 0.411553 7.2:+23364131 MS.gene73381:intron
TGGGATTTAATAGTTGTTGT+TGG 0.429033 7.2:-23363160 None:intergenic
CTGCCATGAGTGTAGTTTGT+TGG 0.432672 7.2:-23363060 None:intergenic
AACTTTGCAGTGGTTGATAG+AGG 0.442912 7.2:+23363474 MS.gene73381:CDS
AAGGCACCAAGTCAAGTTGC+CGG 0.443037 7.2:+23363423 MS.gene73381:CDS
GAAGAGAACGGAGAGGGAGA+TGG 0.458645 7.2:-23363028 None:intergenic
AGCATCATCTTGGCAGCCAT+TGG 0.466070 7.2:-23362987 None:intergenic
GTTAACCATCTTAAGTCCAT+TGG 0.471533 7.2:+23364274 MS.gene73381:CDS
TTACGCCAATGGACTTAAGA+TGG 0.483906 7.2:-23364279 None:intergenic
GAGGGAGATGGTGTGGATGT+GGG 0.487288 7.2:-23363016 None:intergenic
AAGGAGTGATGGTTCCCAGC+AGG 0.487689 7.2:+23364150 MS.gene73381:CDS
AACCAATTATTGCCAACATC+CGG 0.490534 7.2:-23364248 None:intergenic
ACCTCCCCGGCAACTTGACT+TGG 0.492320 7.2:-23363429 None:intergenic
ACCATAACCGTGCCGGATGT+TGG 0.492740 7.2:+23364236 MS.gene73381:CDS
AGGCACCAAGTCAAGTTGCC+GGG 0.499075 7.2:+23363424 MS.gene73381:CDS
TGGGGACAACAGCATCATCT+TGG 0.515410 7.2:-23362997 None:intergenic
AGGGAGATGGTGTGGATGTG+GGG 0.515801 7.2:-23363015 None:intergenic
TTGTTACTTACAGAGACTAC+TGG 0.521171 7.2:+23363191 MS.gene73381:CDS
TTTCTGCACTTGCATCAGCC+TGG 0.522655 7.2:+23364193 MS.gene73381:CDS
GAACGGAGAGGGAGATGGTG+TGG 0.523786 7.2:-23363023 None:intergenic
AATGCCTTCACTTACGCCAA+TGG 0.524915 7.2:-23364290 None:intergenic
GAAGTGGAAGAGAACGGAGA+GGG 0.529733 7.2:-23363034 None:intergenic
TGGAGTGAAGTGGAAGAGAA+CGG 0.534314 7.2:-23363040 None:intergenic
ATGAAGGTTTGAAGGAGTGA+TGG 0.536342 7.2:+23364139 MS.gene73381:intron
TGTAGTTTGTTGGAGTGAAG+TGG 0.546339 7.2:-23363050 None:intergenic
AGGAGTGATGGTTCCCAGCA+GGG 0.555627 7.2:+23364151 MS.gene73381:CDS
GCACAAGGGAAACACTTGCC+AGG 0.561065 7.2:-23364211 None:intergenic
TACTCGTAATAACTTTGCAG+TGG 0.569315 7.2:+23363464 MS.gene73381:CDS
GCACGGTTATGGTGAGCACA+AGG 0.570393 7.2:-23364226 None:intergenic
GGAGTGATGGTTCCCAGCAG+GGG 0.585564 7.2:+23364152 MS.gene73381:CDS
TGTGCTCACCATAACCGTGC+CGG 0.593329 7.2:+23364229 MS.gene73381:CDS
GAATTCAGCACTCTCGTGAA+GGG 0.593669 7.2:-23363126 None:intergenic
CAAACGTTCTGTCAAATCCA+AGG 0.596958 7.2:+23363286 MS.gene73381:CDS
ACTCCAACAAACTACACTCA+TGG 0.600149 7.2:+23363057 MS.gene73381:CDS
TGAATTCAGCACTCTCGTGA+AGG 0.606564 7.2:-23363127 None:intergenic
TTGATGAGAGACCAAAGACC+AGG 0.611561 7.2:-23363266 None:intergenic
AAAGAATGATAGTACCTCCC+CGG 0.616255 7.2:-23363442 None:intergenic
TGAAGTGGAAGAGAACGGAG+AGG 0.617407 7.2:-23363035 None:intergenic
ACCAAGTCAAGTTGCCGGGG+AGG 0.629980 7.2:+23363428 MS.gene73381:CDS
ATTATTGCCAACATCCGGCA+CGG 0.632668 7.2:-23364243 None:intergenic
GAGTGATGGTTCCCAGCAGG+GGG 0.640157 7.2:+23364153 MS.gene73381:CDS
GGCACCAAGTCAAGTTGCCG+GGG 0.643673 7.2:+23363425 MS.gene73381:CDS
CACGGTTATGGTGAGCACAA+GGG 0.668400 7.2:-23364225 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGACATTAATAACTAAA+TGG - chr7.2:23364010-23364029 None:intergenic 15.0%
!! ATATGTTAACACCAAATAAT+GGG - chr7.2:23363971-23363990 None:intergenic 20.0%
!! ATTAATTAAGAGGCAAAAAT+AGG - chr7.2:23364069-23364088 None:intergenic 20.0%
!! CAACAAAAAAAAAAACAAAG+TGG - chr7.2:23363339-23363358 None:intergenic 20.0%
!! TCATTAAATTACTAGAATGA+GGG - chr7.2:23363799-23363818 None:intergenic 20.0%
!!! TCTTTTACAACATTAAAGAT+TGG - chr7.2:23363921-23363940 None:intergenic 20.0%
! AATGAATGAATGAATTACCT+TGG - chr7.2:23363306-23363325 None:intergenic 25.0%
! AATGATCAATAGAAGATGTT+TGG + chr7.2:23363558-23363577 MS.gene73381:intron 25.0%
! CAATTAAAGAGAGAATGTAA+TGG - chr7.2:23363520-23363539 None:intergenic 25.0%
! GATATGTTAACACCAAATAA+TGG - chr7.2:23363972-23363991 None:intergenic 25.0%
! GTCATTAAATTACTAGAATG+AGG - chr7.2:23363800-23363819 None:intergenic 25.0%
! TGACGTAATGATTAATTAAG+AGG - chr7.2:23364079-23364098 None:intergenic 25.0%
!! AAGAAGACTTTTATAGATGA+AGG + chr7.2:23363393-23363412 MS.gene73381:CDS 25.0%
!! AATCGAATTTTTACGTTCTT+TGG + chr7.2:23363835-23363854 MS.gene73381:intron 25.0%
!! ACATAGAGACTTTTGATAAA+TGG - chr7.2:23363945-23363964 None:intergenic 25.0%
!!! ACATTAAAGATTGGCTTAAT+TGG - chr7.2:23363912-23363931 None:intergenic 25.0%
!!! TTTTTCTGTTTCTTCTATGA+AGG + chr7.2:23364123-23364142 MS.gene73381:intron 25.0%
CAAGTTAAATGTTCTAACCA+TGG + chr7.2:23363672-23363691 MS.gene73381:intron 30.0%
GATTTCACCAATGCTTAAAA+TGG - chr7.2:23363649-23363668 None:intergenic 30.0%
GCTAGTTATTATACTTCTCT+TGG + chr7.2:23363224-23363243 MS.gene73381:CDS 30.0%
GTAAGTAACAAGAGAGAATT+GGG - chr7.2:23363182-23363201 None:intergenic 30.0%
TACTAACCAAATCTTTGCAT+AGG + chr7.2:23363868-23363887 MS.gene73381:intron 30.0%
TATAGATGAAGGTGGAAATA+AGG + chr7.2:23363404-23363423 MS.gene73381:CDS 30.0%
TATTGATCATTGCAAAAGCA+AGG - chr7.2:23363549-23363568 None:intergenic 30.0%
TGCTTATAATGAAGTCTAGA+GGG + chr7.2:23363619-23363638 MS.gene73381:intron 30.0%
TGGGATTTAATAGTTGTTGT+TGG - chr7.2:23363163-23363182 None:intergenic 30.0%
TGTAAGTAACAAGAGAGAAT+TGG - chr7.2:23363183-23363202 None:intergenic 30.0%
TTGCTTATAATGAAGTCTAG+AGG + chr7.2:23363618-23363637 MS.gene73381:intron 30.0%
TTTCTTCTATGAAGGTTTGA+AGG + chr7.2:23364131-23364150 MS.gene73381:intron 30.0%
! AAGACTTTTATAGATGAAGG+TGG + chr7.2:23363396-23363415 MS.gene73381:CDS 30.0%
! TTGACAATTTCGTGCTTTTA+TGG + chr7.2:23363356-23363375 MS.gene73381:intron 30.0%
!! AAACGAAGTTTCTTTGGTTT+TGG - chr7.2:23363104-23363123 None:intergenic 30.0%
!! ATGTTGGCAATAATTGGTTT+TGG + chr7.2:23364252-23364271 MS.gene73381:CDS 30.0%
!! TACTCGTTGCATTTAATTTG+TGG - chr7.2:23363587-23363606 None:intergenic 30.0%
!!! GAATGAGGGTTTTTTGAATT+CGG - chr7.2:23363785-23363804 None:intergenic 30.0%
!!! TCTTTGGTTTTGGATTTTGA+AGG - chr7.2:23363094-23363113 None:intergenic 30.0%
AACCAATTATTGCCAACATC+CGG - chr7.2:23364251-23364270 None:intergenic 35.0%
AGGGATAAACGAAGTTTCTT+TGG - chr7.2:23363110-23363129 None:intergenic 35.0%
CTCTATGTCTACCCATTATT+TGG + chr7.2:23363957-23363976 MS.gene73381:intron 35.0%
CTTAATTGGTCATAGCAAAC+TGG - chr7.2:23363898-23363917 None:intergenic 35.0%
GTTAACCATCTTAAGTCCAT+TGG + chr7.2:23364274-23364293 MS.gene73381:CDS 35.0%
TAAGAGGCAAAAATAGGAGA+GGG - chr7.2:23364063-23364082 None:intergenic 35.0%
TACTCGTAATAACTTTGCAG+TGG + chr7.2:23363464-23363483 MS.gene73381:CDS 35.0%
TGTAATGGTTACTTACGTGA+TGG - chr7.2:23363505-23363524 None:intergenic 35.0%
TTAAGAGGCAAAAATAGGAG+AGG - chr7.2:23364064-23364083 None:intergenic 35.0%
! GTGATCACAGTTTAAAACCA+TGG - chr7.2:23363692-23363711 None:intergenic 35.0%
! TGGTGTTAACATATCGAGTT+AGG + chr7.2:23363977-23363996 MS.gene73381:intron 35.0%
! TTGTTACTTACAGAGACTAC+TGG + chr7.2:23363191-23363210 MS.gene73381:CDS 35.0%
!! CTTTTTGTTATCTCTGTTCC+TGG + chr7.2:23363248-23363267 MS.gene73381:CDS 35.0%
!!! TTGGTTTTGGATTTTGAAGG+TGG - chr7.2:23363091-23363110 None:intergenic 35.0%
AAAGAATGATAGTACCTCCC+CGG - chr7.2:23363445-23363464 None:intergenic 40.0%
ACTCCAACAAACTACACTCA+TGG + chr7.2:23363057-23363076 MS.gene73381:CDS 40.0%
ATGAAGGTTTGAAGGAGTGA+TGG + chr7.2:23364139-23364158 MS.gene73381:intron 40.0%
CAAACGTTCTGTCAAATCCA+AGG + chr7.2:23363286-23363305 MS.gene73381:CDS 40.0%
GCAAAAATAGGAGAGGGATA+AGG - chr7.2:23364057-23364076 None:intergenic 40.0%
TGTTGCAATTGTTGATACCG+TGG + chr7.2:23363717-23363736 MS.gene73381:intron 40.0%
! AGAGACTACTGGTTACTCTT+TGG + chr7.2:23363202-23363221 MS.gene73381:CDS 40.0%
! TTACGCCAATGGACTTAAGA+TGG - chr7.2:23364282-23364301 None:intergenic 40.0%
! TTATCTCTGTTCCTGGTCTT+TGG + chr7.2:23363255-23363274 MS.gene73381:CDS 40.0%
!! AACTTTGCAGTGGTTGATAG+AGG + chr7.2:23363474-23363493 MS.gene73381:CDS 40.0%
!! GTTTTGGATTTTGAAGGTGG+TGG - chr7.2:23363088-23363107 None:intergenic 40.0%
!! TGTAGTTTGTTGGAGTGAAG+TGG - chr7.2:23363053-23363072 None:intergenic 40.0%
!!! GGGAGTACCATTTTAAGCAT+TGG + chr7.2:23363639-23363658 MS.gene73381:intron 40.0%
ACCGTGGACATAAGCATTTG+TGG + chr7.2:23363733-23363752 MS.gene73381:intron 45.0%
ATTATTGCCAACATCCGGCA+CGG - chr7.2:23364246-23364265 None:intergenic 45.0%
GAATTCAGCACTCTCGTGAA+GGG - chr7.2:23363129-23363148 None:intergenic 45.0%
GCCACAAATGCTTATGTCCA+CGG - chr7.2:23363737-23363756 None:intergenic 45.0%
GGAGCTCCTATGCAAAGATT+TGG - chr7.2:23363877-23363896 None:intergenic 45.0%
TGAATTCAGCACTCTCGTGA+AGG - chr7.2:23363130-23363149 None:intergenic 45.0%
TGCCGGATGTTGGCAATAAT+TGG + chr7.2:23364246-23364265 MS.gene73381:CDS 45.0%
TGGAGTGAAGTGGAAGAGAA+CGG - chr7.2:23363043-23363062 None:intergenic 45.0%
TTGATGAGAGACCAAAGACC+AGG - chr7.2:23363269-23363288 None:intergenic 45.0%
! CTGCCATGAGTGTAGTTTGT+TGG - chr7.2:23363063-23363082 None:intergenic 45.0%
CACGGTTATGGTGAGCACAA+GGG - chr7.2:23364228-23364247 None:intergenic 50.0%
GAAGTGGAAGAGAACGGAGA+GGG - chr7.2:23363037-23363056 None:intergenic 50.0%
TGAAGTGGAAGAGAACGGAG+AGG - chr7.2:23363038-23363057 None:intergenic 50.0%
TGGGGACAACAGCATCATCT+TGG - chr7.2:23363000-23363019 None:intergenic 50.0%
TTTCTGCACTTGCATCAGCC+TGG + chr7.2:23364193-23364212 MS.gene73381:CDS 50.0%
! AAGGCACCAAGTCAAGTTGC+CGG + chr7.2:23363423-23363442 MS.gene73381:CDS 50.0%
AAGGAGTGATGGTTCCCAGC+AGG + chr7.2:23364150-23364169 MS.gene73381:CDS 55.0%
ACCATAACCGTGCCGGATGT+TGG + chr7.2:23364236-23364255 MS.gene73381:CDS 55.0%
AGAGGGAGATGGTGTGGATG+TGG - chr7.2:23363020-23363039 None:intergenic 55.0%
AGGAGTGATGGTTCCCAGCA+GGG + chr7.2:23364151-23364170 MS.gene73381:CDS 55.0%
AGGGAGATGGTGTGGATGTG+GGG - chr7.2:23363018-23363037 None:intergenic 55.0%
GAAGAGAACGGAGAGGGAGA+TGG - chr7.2:23363031-23363050 None:intergenic 55.0%
GAGGGAGATGGTGTGGATGT+GGG - chr7.2:23363019-23363038 None:intergenic 55.0%
GCACAAGGGAAACACTTGCC+AGG - chr7.2:23364214-23364233 None:intergenic 55.0%
GCACGGTTATGGTGAGCACA+AGG - chr7.2:23364229-23364248 None:intergenic 55.0%
TGTGCTCACCATAACCGTGC+CGG + chr7.2:23364229-23364248 MS.gene73381:CDS 55.0%
! AGGCACCAAGTCAAGTTGCC+GGG + chr7.2:23363424-23363443 MS.gene73381:CDS 55.0%
ACCTCCCCGGCAACTTGACT+TGG - chr7.2:23363432-23363451 None:intergenic 60.0%
GAACGGAGAGGGAGATGGTG+TGG - chr7.2:23363026-23363045 None:intergenic 60.0%
GAGTGATGGTTCCCAGCAGG+GGG + chr7.2:23364153-23364172 MS.gene73381:CDS 60.0%
GCCAACATCCGGCACGGTTA+TGG - chr7.2:23364240-23364259 None:intergenic 60.0%
GGAGTGATGGTTCCCAGCAG+GGG + chr7.2:23364152-23364171 MS.gene73381:CDS 60.0%
! ACCAAGTCAAGTTGCCGGGG+AGG + chr7.2:23363428-23363447 MS.gene73381:CDS 60.0%
! GGCACCAAGTCAAGTTGCCG+GGG + chr7.2:23363425-23363444 MS.gene73381:CDS 60.0%
!! AGTGCTGCTTGCCCCCTGCT+GGG - chr7.2:23364167-23364186 None:intergenic 65.0%
!! CAGTGCTGCTTGCCCCCTGC+TGG - chr7.2:23364168-23364187 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 23362990 23364301 23362990 ID=MS.gene73381
chr7.2 mRNA 23362990 23364301 23362990 ID=MS.gene73381.t1;Parent=MS.gene73381
chr7.2 exon 23362990 23363307 23362990 ID=MS.gene73381.t1.exon1;Parent=MS.gene73381.t1
chr7.2 CDS 23362990 23363307 23362990 ID=cds.MS.gene73381.t1;Parent=MS.gene73381.t1
chr7.2 exon 23363387 23363508 23363387 ID=MS.gene73381.t1.exon2;Parent=MS.gene73381.t1
chr7.2 CDS 23363387 23363508 23363387 ID=cds.MS.gene73381.t1;Parent=MS.gene73381.t1
chr7.2 exon 23364145 23364301 23364145 ID=MS.gene73381.t1.exon3;Parent=MS.gene73381.t1
chr7.2 CDS 23364145 23364301 23364145 ID=cds.MS.gene73381.t1;Parent=MS.gene73381.t1
Gene Sequence

>MS.gene73381

ATGGCTGCCAAGATGATGCTGTTGTCCCCACATCCACACCATCTCCCTCTCCGTTCTCTTCCACTTCACTCCAACAAACTACACTCATGGCAGCACCACCACCTTCAAAATCCAAAACCAAAGAAACTTCGTTTATCCCTTCACGAGAGTGCTGAATTCATCACACAAAACCAACAACAACTATTAAATCCCAATTCTCTCTTGTTACTTACAGAGACTACTGGTTACTCTTTGGCTAGTTATTATACTTCTCTTGGTCTTTTTGTTATCTCTGTTCCTGGTCTTTGGTCTCTCATCAAACGTTCTGTCAAATCCAAGGTAATTCATTCATTCATTCATTTCATTTCCACTTTGTTTTTTTTTTTGTTGACAATTTCGTGCTTTTATGGAAAAATAGATTGTGAAGAAGACTTTTATAGATGAAGGTGGAAATAAGGCACCAAGTCAAGTTGCCGGGGAGGTACTATCATTCTTTACTCGTAATAACTTTGCAGTGGTTGATAGAGGAGAAACCATCACGTAAGTAACCATTACATTCTCTCTTTAATTGCATTCTCCTTGCTTTTGCAATGATCAATAGAAGATGTTTGGAATCCACAAATTAAATGCAACGAGTAAGCAGATAGTTTTGCTTATAATGAAGTCTAGAGGGAGTACCATTTTAAGCATTGGTGAAATCAATCAAGTTAAATGTTCTAACCATGGTTTTAAACTGTGATCACATTCGTGTTGCAATTGTTGATACCGTGGACATAAGCATTTGTGGCGAAGCAATTTCAATAGTGACGATCACCGAATTCAAAAAACCCTCATTCTAGTAATTTAATGACATTTTGAAAATAACTAATCGAATTTTTACGTTCTTTGGTGAAAGATTTTACTAACCAAATCTTTGCATAGGAGCTCCAGTTTGCTATGACCAATTAAGCCAATCTTTAATGTTGTAAAAGAACCATTTATCAAAAGTCTCTATGTCTACCCATTATTTGGTGTTAACATATCGAGTTAGGAATCTCTCCATTTAGTTATTAATGTCATTAAGTTACTGATATATTGTACTTCAACCTTATCCCTCTCCTATTTTTGCCTCTTAATTAATCATTACGTCATATGAAGATAAACATCTAAACAAGTTTTTCTGTTTCTTCTATGAAGGTTTGAAGGAGTGATGGTTCCCAGCAGGGGGCAAGCAGCACTGTTAACTTTCTGCACTTGCATCAGCCTGGCAAGTGTTTCCCTTGTGCTCACCATAACCGTGCCGGATGTTGGCAATAATTGGTTTTGGTTAACCATCTTAAGTCCATTGGCGTAA

Protein sequence

>MS.gene73381.t1

MAAKMMLLSPHPHHLPLRSLPLHSNKLHSWQHHHLQNPKPKKLRLSLHESAEFITQNQQQLLNPNSLLLLTETTGYSLASYYTSLGLFVISVPGLWSLIKRSVKSKIVKKTFIDEGGNKAPSQVAGEVLSFFTRNNFAVVDRGETITFEGVMVPSRGQAALLTFCTCISLASVSLVLTITVPDVGNNWFWLTILSPLA