AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94829


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94829.t1 MTR_1g069465 100.000 182 0 0 1 182 1 182 1.80e-136 378
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94829.t1 AT4G28660 68.966 145 45 0 37 181 37 181 1.03e-71 215
MS.gene94829.t1 AT4G28660 68.966 145 45 0 37 181 52 196 2.52e-71 214

Find 42 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTGAATGAATTGAATTT+TGG 0.043355 1.4:+56026979 None:intergenic
GGAGAAGTAGGCGACATTAC+TGG 0.365446 1.4:-56026851 MS.gene94829:CDS
CCCAAAATTCAATTCATTCA+AGG 0.375868 1.4:-56026980 MS.gene94829:CDS
AACTTAAATTCCTCATTCAA+TGG 0.412356 1.4:-56027091 MS.gene94829:CDS
TTCCCCGCCATCATGATAAC+CGG 0.429760 1.4:+56027004 None:intergenic
TGGAGTCCGCATTATATACT+TGG 0.434350 1.4:+56026742 None:intergenic
CAAATGGCATGGCTATCTTC+AGG 0.434591 1.4:-56026904 MS.gene94829:CDS
ATTTGCATGTGCCAAGTTTG+AGG 0.436765 1.4:-56027063 MS.gene94829:CDS
TAATGCGGACTCCACGGGAC+TGG 0.442232 1.4:-56026733 MS.gene94829:CDS
AAATCAAAGGATGGAACAAA+TGG 0.447952 1.4:-56026920 MS.gene94829:CDS
TTTGATTCTTCTGGAGAAGT+AGG 0.453284 1.4:-56026863 MS.gene94829:CDS
GGATAGGTTCATGAGATTCA+TGG 0.460311 1.4:-56026711 MS.gene94829:CDS
CATTTACATCAACAGATTGA+AGG 0.465513 1.4:+56026801 None:intergenic
GCCACTTCTTTAATTATCCA+TGG 0.476745 1.4:-56027121 MS.gene94829:CDS
GAGCGATACTCTGATGCTAA+TGG 0.480616 1.4:-56026689 MS.gene94829:CDS
AATTTAAGTTCAAACTTCCA+TGG 0.492114 1.4:+56027104 None:intergenic
CATGATAACCGGCATGTGCA+TGG 0.501061 1.4:+56027015 None:intergenic
ATGCCGGTTATCATGATGGC+GGG 0.504435 1.4:-56027007 MS.gene94829:CDS
CAGAAGCCACGCTCATACTC+AGG 0.512959 1.4:-56027858 MS.gene94829:intron
AATGCGGACTCCACGGGACT+GGG 0.524053 1.4:-56026732 MS.gene94829:CDS
GGACTCCACGGGACTGGGAT+AGG 0.532501 1.4:-56026727 MS.gene94829:CDS
GTATATAATGCGGACTCCAC+GGG 0.541871 1.4:-56026738 MS.gene94829:CDS
GCACATGCCGGTTATCATGA+TGG 0.545209 1.4:-56027011 MS.gene94829:CDS
ATACTTGGCTTCAATTCCTG+AGG 0.546032 1.4:+56026757 None:intergenic
ATCATCGGTAACCTCAAACT+TGG 0.546137 1.4:+56027052 None:intergenic
ATGATAACCGGCATGTGCAT+GGG 0.547496 1.4:+56027016 None:intergenic
AATTGAGTTGTAGAGTGGAG+AGG 0.552148 1.4:+56027881 None:intergenic
TCCATGGATAATTAAAGAAG+TGG 0.555425 1.4:+56027120 None:intergenic
TTTGTCAACGGAAAGCCCTC+AGG 0.559746 1.4:-56026773 MS.gene94829:CDS
AGTATATAATGCGGACTCCA+CGG 0.565595 1.4:-56026739 MS.gene94829:CDS
TACTTGGCTTCAATTCCTGA+GGG 0.571164 1.4:+56026758 None:intergenic
AAGCTGACAAAATCAAAGGA+TGG 0.597295 1.4:-56026929 MS.gene94829:CDS
GTAAATGCTAAATTTGTCAA+CGG 0.597700 1.4:-56026785 MS.gene94829:CDS
CAAATGTTGACCATTGAATG+AGG 0.613868 1.4:+56027081 None:intergenic
TGCCGGTTATCATGATGGCG+GGG 0.623175 1.4:-56027006 MS.gene94829:CDS
AAAGGATGGAACAAATGGCA+TGG 0.632758 1.4:-56026915 MS.gene94829:CDS
CATGCCGGTTATCATGATGG+CGG 0.658092 1.4:-56027008 MS.gene94829:CDS
TGTGAAGCTGACAAAATCAA+AGG 0.662632 1.4:-56026933 MS.gene94829:CDS
TTCTGAATTGAGTTGTAGAG+TGG 0.666148 1.4:+56027876 None:intergenic
ATGAACCTATCCCAGTCCCG+TGG 0.667895 1.4:+56026722 None:intergenic
TTTGTACCTGAGTATGAGCG+TGG 0.677678 1.4:+56027852 None:intergenic
TTGAAGCCAAGTATATAATG+CGG 0.690031 1.4:-56026748 MS.gene94829:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAACAAAACAAAAGAT+GGG - chr1.4:56026970-56026989 MS.gene94829:CDS 15.0%
!! CAAATAAACAAAACAAAAGA+TGG - chr1.4:56026969-56026988 MS.gene94829:CDS 20.0%
!! GTAAATTCATAATATGAATC+AGG - chr1.4:56026869-56026888 MS.gene94829:CDS 20.0%
!! TTAATTTACAAGTCAACAAT+TGG + chr1.4:56026932-56026951 None:intergenic 20.0%
!!! CATCTTTTGTTTTGTTTATT+TGG + chr1.4:56026971-56026990 None:intergenic 20.0%
! AACTTAAATTCCTCATTCAA+TGG - chr1.4:56027485-56027504 MS.gene94829:intron 25.0%
! AGAGAATACTTGAAACTAAT+GGG + chr1.4:56027272-56027291 None:intergenic 25.0%
! ATCATATGTTCAAGATTTCA+AGG + chr1.4:56027344-56027363 None:intergenic 25.0%
! GTAAATGCTAAATTTGTCAA+CGG - chr1.4:56027791-56027810 MS.gene94829:intron 25.0%
! TAGAGAATACTTGAAACTAA+TGG + chr1.4:56027273-56027292 None:intergenic 25.0%
! TGTTGTCAAGAAAAAAATGT+TGG + chr1.4:56027026-56027045 None:intergenic 25.0%
! TTATACTTGTACTAGAATTG+TGG - chr1.4:56027165-56027184 MS.gene94829:intron 25.0%
!! AATTTAAGTTCAAACTTCCA+TGG + chr1.4:56027475-56027494 None:intergenic 25.0%
!! ATGAATCAGGTTAACATTTT+TGG - chr1.4:56026882-56026901 MS.gene94829:CDS 25.0%
!! CCTTGAATGAATTGAATTTT+GGG + chr1.4:56027599-56027618 None:intergenic 25.0%
!! TCCTTGAATGAATTGAATTT+TGG + chr1.4:56027600-56027619 None:intergenic 25.0%
!! TGAATCAGGTTAACATTTTT+GGG - chr1.4:56026883-56026902 MS.gene94829:CDS 25.0%
!! TGGTTTTTATATGATTGATG+AGG - chr1.4:56027745-56027764 MS.gene94829:intron 25.0%
!! TTTTATATGATTGATGAGGA+AGG - chr1.4:56027749-56027768 MS.gene94829:intron 25.0%
!!! ATGTTTTGAGAACTTATGTT+TGG - chr1.4:56026801-56026820 MS.gene94829:CDS 25.0%
!!! CTTTGTTTGTTATTGTTTTG+TGG - chr1.4:56026768-56026787 MS.gene94829:CDS 25.0%
!!! GCATCACTTTTTATTTTCTA+TGG + chr1.4:56027385-56027404 None:intergenic 25.0%
AAATCAAAGGATGGAACAAA+TGG - chr1.4:56027656-56027675 MS.gene94829:intron 30.0%
AAGTTCTCAAAACATTTCAC+AGG + chr1.4:56026796-56026815 None:intergenic 30.0%
CAATTGGAGCAAACTATATA+AGG + chr1.4:56026916-56026935 None:intergenic 30.0%
CATTTACATCAACAGATTGA+AGG + chr1.4:56027778-56027797 None:intergenic 30.0%
CCCAAAATTCAATTCATTCA+AGG - chr1.4:56027596-56027615 MS.gene94829:intron 30.0%
TCCATGGATAATTAAAGAAG+TGG + chr1.4:56027459-56027478 None:intergenic 30.0%
TTCTTGCATTGTTCAGTATT+TGG - chr1.4:56027050-56027069 MS.gene94829:CDS 30.0%
TTGAAGCCAAGTATATAATG+CGG - chr1.4:56027828-56027847 MS.gene94829:intron 30.0%
! GAATCAGGTTAACATTTTTG+GGG - chr1.4:56026884-56026903 MS.gene94829:CDS 30.0%
!!! GGAGTTTTTTGAGTAATCAT+CGG + chr1.4:56027542-56027561 None:intergenic 30.0%
!!! GTTTTGTTTATTTGGCATTC+TGG + chr1.4:56026963-56026982 None:intergenic 30.0%
AAGCTGACAAAATCAAAGGA+TGG - chr1.4:56027647-56027666 MS.gene94829:intron 35.0%
AGAACATCATACATACCATC+AGG + chr1.4:56027102-56027121 None:intergenic 35.0%
ATGTTCAAGATTTCAAGGTC+AGG + chr1.4:56027339-56027358 None:intergenic 35.0%
CAAATGTTGACCATTGAATG+AGG + chr1.4:56027498-56027517 None:intergenic 35.0%
CCAGAAGAATCAAAAACTGA+GGG + chr1.4:56027707-56027726 None:intergenic 35.0%
GAACATCATACATACCATCA+GGG + chr1.4:56027101-56027120 None:intergenic 35.0%
GATGGTATGTATGATGTTCT+TGG - chr1.4:56027102-56027121 MS.gene94829:CDS 35.0%
GCCACTTCTTTAATTATCCA+TGG - chr1.4:56027455-56027474 MS.gene94829:intron 35.0%
TACTAGAATTGTGGAGTAAC+AGG - chr1.4:56027174-56027193 MS.gene94829:intron 35.0%
TCCAGAAGAATCAAAAACTG+AGG + chr1.4:56027708-56027727 None:intergenic 35.0%
TGTGAAGCTGACAAAATCAA+AGG - chr1.4:56027643-56027662 MS.gene94829:intron 35.0%
TGTTCAAGATTTCAAGGTCA+GGG + chr1.4:56027338-56027357 None:intergenic 35.0%
! TTCTGAATTGAGTTGTAGAG+TGG + chr1.4:56026703-56026722 None:intergenic 35.0%
! TTTGATTCTTCTGGAGAAGT+AGG - chr1.4:56027713-56027732 MS.gene94829:intron 35.0%
!!! GGTTAACATTTTTGGGGTTA+AGG - chr1.4:56026890-56026909 MS.gene94829:CDS 35.0%
AAAGGATGGAACAAATGGCA+TGG - chr1.4:56027661-56027680 MS.gene94829:intron 40.0%
AGTATATAATGCGGACTCCA+CGG - chr1.4:56027837-56027856 MS.gene94829:intron 40.0%
ATACTTGGCTTCAATTCCTG+AGG + chr1.4:56027822-56027841 None:intergenic 40.0%
ATCATCGGTAACCTCAAACT+TGG + chr1.4:56027527-56027546 None:intergenic 40.0%
ATGTTCTTGGCAACACTCTA+TGG - chr1.4:56027115-56027134 MS.gene94829:CDS 40.0%
ATTTGCATGTGCCAAGTTTG+AGG - chr1.4:56027513-56027532 MS.gene94829:intron 40.0%
CAACACTCTATGGAACAGAA+TGG - chr1.4:56027125-56027144 MS.gene94829:intron 40.0%
CTATGGAACAGAATGGCATT+GGG - chr1.4:56027132-56027151 MS.gene94829:intron 40.0%
GGATAGGTTCATGAGATTCA+TGG - chr1.4:56027865-56027884 MS.gene94829:CDS 40.0%
GTTCAAGATTTCAAGGTCAG+GGG + chr1.4:56027337-56027356 None:intergenic 40.0%
TAATATAGCTTCCACCCTGA+TGG - chr1.4:56027084-56027103 MS.gene94829:CDS 40.0%
TACTTGGCTTCAATTCCTGA+GGG + chr1.4:56027821-56027840 None:intergenic 40.0%
TCTATGGAACAGAATGGCAT+TGG - chr1.4:56027131-56027150 MS.gene94829:intron 40.0%
TGGAGTCCGCATTATATACT+TGG + chr1.4:56027837-56027856 None:intergenic 40.0%
! AATTGAGTTGTAGAGTGGAG+AGG + chr1.4:56026698-56026717 None:intergenic 40.0%
! GATTTTGTCAGCTTCACATC+AGG + chr1.4:56027641-56027660 None:intergenic 40.0%
!! CCCTCAGTTTTTGATTCTTC+TGG - chr1.4:56027704-56027723 MS.gene94829:intron 40.0%
AAAAACTCCCATGCACATGC+CGG - chr1.4:56027553-56027572 MS.gene94829:intron 45.0%
ATGATAACCGGCATGTGCAT+GGG + chr1.4:56027563-56027582 None:intergenic 45.0%
ATTTCAAGGTCAGGGGTCAA+GGG + chr1.4:56027330-56027349 None:intergenic 45.0%
CAAATGGCATGGCTATCTTC+AGG - chr1.4:56027672-56027691 MS.gene94829:intron 45.0%
CATCATACATACCATCAGGG+TGG + chr1.4:56027098-56027117 None:intergenic 45.0%
GAGCGATACTCTGATGCTAA+TGG - chr1.4:56027887-56027906 MS.gene94829:CDS 45.0%
GTATATAATGCGGACTCCAC+GGG - chr1.4:56027838-56027857 MS.gene94829:intron 45.0%
TTTGTACCTGAGTATGAGCG+TGG + chr1.4:56026727-56026746 None:intergenic 45.0%
CATGATAACCGGCATGTGCA+TGG + chr1.4:56027564-56027583 None:intergenic 50.0%
GATTTCAAGGTCAGGGGTCA+AGG + chr1.4:56027331-56027350 None:intergenic 50.0%
GGAGAAGTAGGCGACATTAC+TGG - chr1.4:56027725-56027744 MS.gene94829:intron 50.0%
TTCCCCGCCATCATGATAAC+CGG + chr1.4:56027575-56027594 None:intergenic 50.0%
TTTGTCAACGGAAAGCCCTC+AGG - chr1.4:56027803-56027822 MS.gene94829:intron 50.0%
! ATGCCGGTTATCATGATGGC+GGG - chr1.4:56027569-56027588 MS.gene94829:intron 50.0%
! CATGCCGGTTATCATGATGG+CGG - chr1.4:56027568-56027587 MS.gene94829:intron 50.0%
! GCACATGCCGGTTATCATGA+TGG - chr1.4:56027565-56027584 MS.gene94829:intron 50.0%
ATGAACCTATCCCAGTCCCG+TGG + chr1.4:56027857-56027876 None:intergenic 55.0%
CAGAAGCCACGCTCATACTC+AGG - chr1.4:56026718-56026737 MS.gene94829:CDS 55.0%
! TGCCGGTTATCATGATGGCG+GGG - chr1.4:56027570-56027589 MS.gene94829:intron 55.0%
AATGCGGACTCCACGGGACT+GGG - chr1.4:56027844-56027863 MS.gene94829:intron 60.0%
TAATGCGGACTCCACGGGAC+TGG - chr1.4:56027843-56027862 MS.gene94829:intron 60.0%
GGACTCCACGGGACTGGGAT+AGG - chr1.4:56027849-56027868 MS.gene94829:intron 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 56026667 56027931 56026667 ID=MS.gene94829
chr1.4 mRNA 56026667 56027931 56026667 ID=MS.gene94829.t1;Parent=MS.gene94829
chr1.4 exon 56027859 56027931 56027859 ID=MS.gene94829.t1.exon1;Parent=MS.gene94829.t1
chr1.4 CDS 56027859 56027931 56027859 ID=cds.MS.gene94829.t1;Parent=MS.gene94829.t1
chr1.4 exon 56026667 56027142 56026667 ID=MS.gene94829.t1.exon2;Parent=MS.gene94829.t1
chr1.4 CDS 56026667 56027142 56026667 ID=cds.MS.gene94829.t1;Parent=MS.gene94829.t1
Gene Sequence

>MS.gene94829

ATGGCAACTATGCACTCTCTTGCATTCTCCTCTCCACTCTACAACTCAATTCAGAAGCCACGCTCATACTCAGGTACAAACACTCTCTTCAACATTACTTTCTTTGTTTGTTATTGTTTTGTGGTTCCTGTGAAATGTTTTGAGAACTTATGTTTGGACTATTCAATTTTATAACAGTTGTCAGATTTTTAAAAAAATAACAGTAAATTCATAATATGAATCAGGTTAACATTTTTGGGGTTAAGGCCTTATATAGTTTGCTCCAATTGTTGACTTGTAAATTAATGTGCTTTCCAGAATGCCAAATAAACAAAACAAAAGATGGGTGACATTATTTTCTTTTGTTTATCTTTTTGCCAACATTTTTTTCTTGACAACATTAATTCTTGCATTGTTCAGTATTTGGAAACAATAATTTAATATAGCTTCCACCCTGATGGTATGTATGATGTTCTTGGCAACACTCTATGGAACAGAATGGCATTGGGAAGTTTTGAGTTATACTTGTACTAGAATTGTGGAGTAACAGGACAATAGAAAATTTGCAATTAATAACAAAATTGTACTTTTGCAATGTATGAACATGAAGTAATCTTAGAATTCCCATTAGTTTCAAGTATTCTCTATACATGTAGAGCAATTTCTAGTATATCATTGCTTCCCTTGACCCCTGACCTTGAAATCTTGAACATATGATTTCTTTGAAGATTTTTAGCCATAGAAAATAAAAAGTGATGCATATGATATATGATATGCATGATGAAAATTATAGACTATTTGTTTATGCAGCCACTTCTTTAATTATCCATGGAAGTTTGAACTTAAATTCCTCATTCAATGGTCAACATTTGCATGTGCCAAGTTTGAGGTTACCGATGATTACTCAAAAAACTCCCATGCACATGCCGGTTATCATGATGGCGGGGAAGCCCAAAATTCAATTCATTCAAGGAACTGATGAACTGACAATACCTGATGTGAAGCTGACAAAATCAAAGGATGGAACAAATGGCATGGCTATCTTCAGGTTTGATCAACCCTCAGTTTTTGATTCTTCTGGAGAAGTAGGCGACATTACTGGTTTTTATATGATTGATGAGGAAGGAGTCCTTCAATCTGTTGATGTAAATGCTAAATTTGTCAACGGAAAGCCCTCAGGAATTGAAGCCAAGTATATAATGCGGACTCCACGGGACTGGGATAGGTTCATGAGATTCATGGAGCGATACTCTGATGCTAATGGTTTGCAATTCATAAAATATTGA

Protein sequence

>MS.gene94829.t1

MATMHSLAFSSPLYNSIQKPRSYSATSLIIHGSLNLNSSFNGQHLHVPSLRLPMITQKTPMHMPVIMMAGKPKIQFIQGTDELTIPDVKLTKSKDGTNGMAIFRFDQPSVFDSSGEVGDITGFYMIDEEGVLQSVDVNAKFVNGKPSGIEAKYIMRTPRDWDRFMRFMERYSDANGLQFIKY