AlfalfaGEDB | MsG0480018580.01

M. sativa cultivar ZhongmuNo.1 / MsG0480018580.01

KEGG: -
GO: -
PFAM: PF05097

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018580.01.T01	XP_003604575.1	96.911	259	7	1	1	259	17	274	0	517

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018580.01.T01	G7JLT9	96.911	259	7	1	1	259	17	274	0.0	517

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048273.01	MsG0480018580.01	0.806216	9.163001e-50	4.945557e-47
MsG0080048528.01	MsG0480018580.01	0.824791	6.665087e-54	5.964678e-51
MsG0080048801.01	MsG0480018580.01	0.800961	1.127159e-48	5.318788e-46
MsG0080048845.01	MsG0480018580.01	0.844004	9.887994e-59	1.574859e-55
MsG0080048969.01	MsG0480018580.01	0.800716	1.264753e-48	5.930568e-46
MsG0080049004.01	MsG0480018580.01	0.812260	4.637280e-51	2.934431e-48
MsG0080049078.01	MsG0480018580.01	0.816441	5.521356e-52	3.912434e-49
MsG0080049129.01	MsG0480018580.01	0.822003	2.989117e-53	2.470940e-50
MsG0080049130.01	MsG0480018580.01	0.844785	6.095466e-59	9.949448e-56
MsG0180000017.01	MsG0480018580.01	0.818150	2.277762e-52	1.691472e-49
MsG0180000638.01	MsG0480018580.01	0.814049	1.877191e-51	1.246361e-48
MsG0180000843.01	MsG0480018580.01	0.857099	2.054968e-62	5.014280e-59
MsG0180001371.01	MsG0480018580.01	0.810473	1.132945e-50	6.835623e-48
MsG0180001685.01	MsG0480018580.01	0.811538	6.658179e-51	4.132424e-48
MsG0180001929.01	MsG0480018580.01	0.816412	5.604453e-52	3.968309e-49
MsG0180002982.01	MsG0480018580.01	0.803367	3.606419e-49	1.808924e-46
MsG0180003316.01	MsG0480018580.01	0.830832	2.352216e-55	2.507171e-52
MsG0180003352.01	MsG0480018580.01	0.807213	5.640133e-50	3.123197e-47
MsG0180003451.01	MsG0480018580.01	0.811510	6.751913e-51	4.187649e-48
MsG0180003453.01	MsG0480018580.01	0.808340	3.250187e-50	1.853684e-47
MsG0180003933.01	MsG0480018580.01	0.835455	1.661747e-56	2.033128e-53
MsG0180004662.01	MsG0480018580.01	0.828133	1.065095e-54	1.049320e-51
MsG0180004934.01	MsG0480018580.01	0.808675	2.756680e-50	1.585930e-47
MsG0180004987.01	MsG0480018580.01	0.822585	2.189753e-53	1.840683e-50
MsG0180005039.01	MsG0480018580.01	0.800187	1.620849e-48	7.499204e-46
MsG0180005119.01	MsG0480018580.01	0.815958	7.080650e-52	4.951678e-49
MsG0180005150.01	MsG0480018580.01	0.805150	1.534114e-49	8.056510e-47
MsG0180005276.01	MsG0480018580.01	0.816397	5.648832e-52	3.998146e-49
MsG0180005283.01	MsG0480018580.01	0.829359	5.379882e-55	5.494054e-52
MsG0180005349.01	MsG0480018580.01	0.810227	1.280131e-50	7.673350e-48
MsG0180005561.01	MsG0480018580.01	0.819463	1.146217e-52	8.826988e-50
MsG0180005803.01	MsG0480018580.01	0.816011	6.889977e-52	4.825524e-49
MsG0180005879.01	MsG0480018580.01	0.817116	3.895973e-52	2.812272e-49
MsG0180005969.01	MsG0480018580.01	0.805729	1.159538e-49	6.181161e-47
MsG0180006223.01	MsG0480018580.01	0.809587	1.757583e-50	1.036089e-47
MsG0280010926.01	MsG0480018580.01	0.820471	6.739551e-53	5.337681e-50
MsG0280010998.01	MsG0480018580.01	0.803848	2.866239e-49	1.455665e-46
MsG0280011270.01	MsG0480018580.01	0.837697	4.460278e-57	5.840866e-54
MsG0280011411.01	MsG0480018580.01	0.840394	8.928008e-58	1.270058e-54
MsG0380011495.01	MsG0480018580.01	0.805037	1.619651e-49	8.480883e-47
MsG0380011632.01	MsG0480018580.01	0.839464	1.560473e-57	2.156694e-54
MsG0380013111.01	MsG0480018580.01	0.808007	3.826703e-50	2.163302e-47
MsG0380013177.01	MsG0480018580.01	0.853427	2.407654e-61	5.200846e-58
MsG0380013472.01	MsG0480018580.01	0.805353	1.390730e-49	7.341650e-47
MsG0380013530.01	MsG0480018580.01	0.809594	1.751937e-50	1.032927e-47
MsG0380013955.01	MsG0480018580.01	0.810292	1.239667e-50	7.443418e-48
MsG0380013970.01	MsG0480018580.01	0.823681	1.214821e-53	1.053346e-50
MsG0380013992.01	MsG0480018580.01	0.801476	8.843904e-49	4.227624e-46
MsG0380014007.01	MsG0480018580.01	0.816498	5.360436e-52	3.804418e-49
MsG0380014022.01	MsG0480018580.01	0.814880	1.229885e-51	8.350961e-49
MsG0380014053.01	MsG0480018580.01	0.817264	3.608040e-52	2.614976e-49
MsG0480018580.01	MsG0480018631.01	0.806681	7.308977e-50	3.991712e-47
MsG0480018580.01	MsG0480019485.01	0.803794	2.940702e-49	1.491360e-46
MsG0480018580.01	MsG0480019573.01	0.802862	4.585921e-49	2.270861e-46
MsG0480018580.01	MsG0480019592.01	0.841508	4.553222e-58	6.705237e-55
MsG0480018580.01	MsG0480019971.01	0.802090	6.616392e-49	3.212425e-46
MsG0480018580.01	MsG0480019974.01	0.850554	1.575802e-60	3.098343e-57
MsG0480018580.01	MsG0480020350.01	0.833546	5.011609e-56	5.790359e-53
MsG0480018580.01	MsG0480021168.01	0.828679	7.861229e-55	7.868631e-52
MsG0480018580.01	MsG0480021207.01	0.808936	2.423683e-50	1.403995e-47
MsG0480018580.01	MsG0480021294.01	0.809604	1.743045e-50	1.027975e-47
MsG0480018580.01	MsG0480021303.01	0.819359	1.210656e-52	9.295171e-50
MsG0480018580.01	MsG0480021377.01	0.800115	1.676921e-48	7.744222e-46
MsG0480018580.01	MsG0480021406.01	0.831902	1.282744e-55	1.411468e-52
MsG0480018580.01	MsG0480021420.01	0.820566	6.409129e-53	5.089109e-50
MsG0480018580.01	MsG0480021777.01	0.800994	1.109792e-48	5.241371e-46
MsG0480018580.01	MsG0480021783.01	0.807081	6.014877e-50	3.319203e-47
MsG0480018580.01	MsG0480021817.01	0.833847	4.216159e-56	4.915775e-53
MsG0480018580.01	MsG0480021875.01	0.805522	1.281960e-49	6.796644e-47
MsG0480018580.01	MsG0480021999.01	0.820775	5.739622e-53	4.584045e-50
MsG0480018580.01	MsG0480022297.01	0.802770	4.792063e-49	2.367399e-46
MsG0480018580.01	MsG0480022617.01	0.810751	9.862411e-51	5.995002e-48
MsG0480018580.01	MsG0480022664.01	0.802698	4.957587e-49	2.444680e-46
MsG0480018580.01	MsG0480022804.01	0.810892	9.193564e-51	5.609656e-48
MsG0480018580.01	MsG0480022805.01	0.845656	3.544068e-59	5.948540e-56
MsG0480018580.01	MsG0480023166.01	0.836315	1.005758e-56	1.263015e-53
MsG0480018580.01	MsG0480023351.01	0.836674	8.149748e-57	1.034313e-53
MsG0480018580.01	MsG0480023371.01	0.801782	7.654002e-49	3.687149e-46
MsG0480018580.01	MsG0480023395.01	0.819808	9.560962e-53	7.434202e-50
MsG0480018580.01	MsG0480023422.01	0.812768	3.589657e-51	2.302226e-48
MsG0480018580.01	MsG0480023502.01	0.809165	2.164957e-50	1.261914e-47
MsG0480018580.01	MsG0480023505.01	0.823255	1.528223e-53	1.309037e-50
MsG0480018580.01	MsG0480023616.01	0.824365	8.397834e-54	7.422707e-51
MsG0480018580.01	MsG0480023821.01	0.817186	3.758266e-52	2.718036e-49
MsG0480018580.01	MsG0480023845.01	0.800068	1.714067e-48	7.906668e-46
MsG0480018580.01	MsG0580024386.01	0.812498	4.113542e-51	2.619627e-48
MsG0480018580.01	MsG0580024567.01	0.814954	1.184302e-51	8.057695e-49
MsG0480018580.01	MsG0580024679.01	0.839375	1.645430e-57	2.267984e-54
MsG0480018580.01	MsG0580024681.01	0.813062	3.095704e-51	2.000984e-48
MsG0480018580.01	MsG0580024763.01	0.809507	1.828932e-50	1.075777e-47
MsG0480018580.01	MsG0580024764.01	0.834594	2.738230e-56	3.264721e-53
MsG0480018580.01	MsG0580024766.01	0.801928	7.143251e-49	3.453931e-46
MsG0480018580.01	MsG0580024836.01	0.829129	6.118370e-55	6.205483e-52
MsG0480018580.01	MsG0580024873.01	0.805642	1.209818e-49	6.434202e-47
MsG0480018580.01	MsG0580024944.01	0.825103	5.626540e-54	5.079891e-51
MsG0480018580.01	MsG0580024982.01	0.815253	1.016171e-51	6.970200e-49
MsG0480018580.01	MsG0580025589.01	0.830465	2.892507e-55	3.049585e-52
MsG0480018580.01	MsG0580025845.01	0.805430	1.339921e-49	7.087467e-47
MsG0480018580.01	MsG0580027082.01	0.812787	3.556544e-51	2.282050e-48
MsG0480018580.01	MsG0580027098.01	0.820547	6.475584e-53	5.139266e-50
MsG0480018580.01	MsG0580027236.01	0.803195	3.915079e-49	1.955186e-46
MsG0480018580.01	MsG0580027899.01	0.810346	1.206370e-50	7.254129e-48
MsG0480018580.01	MsG0580028024.01	0.826555	2.543826e-54	2.393522e-51
MsG0480018580.01	MsG0580028073.01	0.812299	4.545474e-51	2.879393e-48
MsG0480018580.01	MsG0580028111.01	0.805053	1.607172e-49	8.419186e-47
MsG0480018580.01	MsG0580028306.01	0.817595	3.039166e-52	2.222804e-49
MsG0480018580.01	MsG0580028495.01	0.829189	5.916085e-55	6.011466e-52
MsG0480018580.01	MsG0580028925.01	0.840621	7.786644e-58	1.115449e-54
MsG0480018580.01	MsG0580029605.01	0.801092	1.059894e-48	5.018043e-46
MsG0480018580.01	MsG0580029700.01	0.818413	1.986200e-52	1.485838e-49
MsG0480018580.01	MsG0580029719.01	0.821963	3.052101e-53	2.520153e-50
MsG0480018580.01	MsG0580029732.01	0.803416	3.522489e-49	1.769131e-46
MsG0480018580.01	MsG0580029967.01	0.843290	1.534367e-58	2.389631e-55
MsG0480018580.01	MsG0580030063.01	0.811508	6.758803e-51	4.191655e-48
MsG0480018580.01	MsG0680030464.01	0.809615	1.733716e-50	1.022807e-47
MsG0480018580.01	MsG0680030902.01	0.801569	8.464466e-49	4.055654e-46
MsG0480018580.01	MsG0680032006.01	0.819397	1.186831e-52	9.122234e-50
MsG0480018580.01	MsG0680032057.01	0.807729	4.383450e-50	2.460146e-47
MsG0480018580.01	MsG0680032515.01	0.808468	3.051475e-50	1.746203e-47
MsG0480018580.01	MsG0680033901.01	0.807155	5.801809e-50	3.207835e-47
MsG0480018580.01	MsG0680033902.01	0.808589	2.874910e-50	1.650267e-47
MsG0480018580.01	MsG0680035308.01	0.806572	7.708070e-50	4.198044e-47
MsG0480018580.01	MsG0680035664.01	0.819031	1.437940e-52	1.094201e-49
MsG0480018580.01	MsG0680035731.01	0.812218	4.736438e-51	2.993822e-48
MsG0480018580.01	MsG0780037184.01	0.804832	1.787825e-49	9.312744e-47
MsG0480018580.01	MsG0780037638.01	0.800482	1.412021e-48	6.581901e-46
MsG0480018580.01	MsG0780037944.01	0.810083	1.374882e-50	8.210638e-48
MsG0480018580.01	MsG0780037946.01	0.837308	5.611888e-57	7.260346e-54
MsG0480018580.01	MsG0780038417.01	0.835534	1.586362e-56	1.945415e-53
MsG0480018580.01	MsG0780038571.01	0.825958	3.529304e-54	3.265005e-51
MsG0480018580.01	MsG0780038653.01	0.810778	9.731703e-51	5.919800e-48
MsG0480018580.01	MsG0780038986.01	0.814241	1.702881e-51	1.136553e-48
MsG0480018580.01	MsG0780039795.01	0.802029	6.810636e-49	3.301562e-46
MsG0480018580.01	MsG0780039805.01	0.817202	3.726135e-52	2.696078e-49
MsG0480018580.01	MsG0780039859.01	0.806057	9.897509e-50	5.320253e-47
MsG0480018580.01	MsG0780039986.01	0.815094	1.102509e-51	7.529558e-49
MsG0480018580.01	MsG0780040080.01	0.815867	7.418562e-52	5.175032e-49
MsG0480018580.01	MsG0780040319.01	0.830584	2.705517e-55	2.862495e-52
MsG0480018580.01	MsG0780040343.01	0.817991	2.474498e-52	1.829573e-49
MsG0480018580.01	MsG0780041004.01	0.832932	7.126229e-56	8.083438e-53
MsG0480018580.01	MsG0780041092.01	0.813346	2.681906e-51	1.747045e-48
MsG0480018580.01	MsG0780041272.01	0.818444	1.954084e-52	1.463107e-49
MsG0480018580.01	MsG0780041289.01	0.822222	2.658765e-53	2.211663e-50
MsG0480018580.01	MsG0780041385.01	0.812968	3.245576e-51	2.092565e-48
MsG0480018580.01	MsG0780041706.01	0.826866	2.144072e-54	2.035491e-51
MsG0480018580.01	MsG0780041778.01	0.823586	1.278833e-53	1.105748e-50
MsG0480018580.01	MsG0880042290.01	0.800006	1.764628e-48	8.126899e-46
MsG0480018580.01	MsG0880042343.01	0.837641	4.609929e-57	6.026573e-54
MsG0480018580.01	MsG0880042690.01	0.809109	2.225733e-50	1.295407e-47
MsG0480018580.01	MsG0880043009.01	0.813696	2.245454e-51	1.476707e-48
MsG0480018580.01	MsG0880043235.01	0.817981	2.487733e-52	1.838762e-49
MsG0480018580.01	MsG0880043250.01	0.825926	3.590915e-54	3.319056e-51
MsG0480018580.01	MsG0880044105.01	0.820678	6.041764e-53	4.812144e-50
MsG0480018580.01	MsG0880044884.01	0.849025	4.212626e-60	7.880734e-57
MsG0480018580.01	MsG0880045073.01	0.802143	6.450899e-49	3.136310e-46
MsG0480018580.01	MsG0880045261.01	0.822511	2.278209e-53	1.910915e-50
MsG0480018580.01	MsG0880045283.01	0.804793	1.821104e-49	9.476560e-47
MsG0480018580.01	MsG0880045412.01	0.815414	9.357054e-52	6.446933e-49
MsG0480018580.01	MsG0880046048.01	0.857840	1.240683e-62	3.103818e-59
MsG0480018580.01	MsG0880046324.01	0.825038	5.828355e-54	5.252688e-51
MsG0480018580.01	MsG0880046735.01	0.822133	2.787665e-53	2.313085e-50
MsG0480018580.01	MsG0880046764.01	0.825974	3.498024e-54	3.237649e-51
MsG0480018580.01	MsG0880046791.01	0.804964	1.677435e-49	8.767471e-47
MsG0480018580.01	MsG0880046866.01	0.801229	9.936678e-49	4.720449e-46
MsG0480018580.01	MsG0880047111.01	0.832707	8.105616e-56	9.133027e-53
MsG0480018580.01	MsG0880047156.01	0.846388	2.240737e-59	3.850711e-56
MsG0480018580.01	MsG0880047157.01	0.829448	5.119658e-55	5.241180e-52
MsG0480018580.01	MsG0880047160.01	0.806983	6.311688e-50	3.474141e-47
MsG0480018580.01	MsG0880047161.01	0.829223	5.803369e-55	5.902845e-52
MsG0480018580.01	MsG0880047451.01	0.829732	4.367113e-55	4.508651e-52
MsG0480018580.01	MsG0880047540.01	0.812557	3.993407e-51	2.547073e-48
MsG0380015103.01	MsG0480018580.01	0.812982	3.222645e-51	2.078600e-48
MsG0380015496.01	MsG0480018580.01	0.810846	9.409383e-51	5.734107e-48
MsG0380015501.01	MsG0480018580.01	0.814020	1.905684e-51	1.264305e-48
MsG0380016058.01	MsG0480018580.01	0.804701	1.904070e-49	9.884179e-47
MsG0380016284.01	MsG0480018580.01	0.807410	5.125389e-50	2.852734e-47
MsG0380016452.01	MsG0480018580.01	0.834596	2.735967e-56	3.262239e-53
MsG0380016459.01	MsG0480018580.01	0.822954	1.796756e-53	1.526071e-50
MsG0380016542.01	MsG0480018580.01	0.808130	3.603327e-50	2.043670e-47
MsG0380016623.01	MsG0480018580.01	0.842884	1.968673e-58	3.026569e-55
MsG0380016751.01	MsG0480018580.01	0.816762	4.679226e-52	3.345160e-49
MsG0380016762.01	MsG0480018580.01	0.852704	3.876377e-61	8.169667e-58
MsG0380017033.01	MsG0480018580.01	0.803718	3.048985e-49	1.543232e-46
MsG0380017532.01	MsG0480018580.01	0.808174	3.524974e-50	2.001684e-47
MsG0380017976.01	MsG0480018580.01	0.827017	1.972905e-54	1.881331e-51
MsG0480018089.01	MsG0480018580.01	0.828733	7.629113e-55	7.647748e-52
MsG0480018323.01	MsG0480018580.01	0.832226	1.066484e-55	1.184529e-52
MsG0480018324.01	MsG0480018580.01	0.838985	2.077108e-57	2.828806e-54
MsG0480018329.01	MsG0480018580.01	0.803661	3.133672e-49	1.583755e-46
MsG0280006848.01	MsG0480018580.01	0.835480	1.637864e-56	2.005343e-53
MsG0280008227.01	MsG0480018580.01	0.818514	1.883469e-52	1.412862e-49
MsG0280009434.01	MsG0480018580.01	0.804255	2.358141e-49	1.210107e-46
MsG0280009751.01	MsG0480018580.01	0.827031	1.957828e-54	1.867709e-51
MsG0280009863.01	MsG0480018580.01	0.804052	2.599595e-49	1.327162e-46
MsG0280010198.01	MsG0480018580.01	0.805413	1.350854e-49	7.142240e-47
MsG0280010496.01	MsG0480018580.01	0.830670	2.576408e-55	2.733130e-52
MsG0280010523.01	MsG0480018580.01	0.802789	4.749195e-49	2.347358e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018580.01.T01	MTR_4g014670	96.911	259	7	1	1	259	17	274	0.0	517
MsG0480018580.01.T01	MTR_7g069710	46.324	272	89	13	1	249	23	260	2.64e-51	169

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 35 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCTCGAAACTTTCTTCCCT+TGG	0.318412	4:-6892423	MsG0480018580.01.T01:CDS
TCTTTGTCATCAAGTTCATT+AGG	0.362522	4:-6892976	MsG0480018580.01.T01:CDS
GATTCTGATAAATAGCATTC+TGG	0.384127	4:+6892511	None:intergenic
CCATTCCTACCAGTATCTTT+CGG	0.386168	4:+6892895	None:intergenic
TCTTCCGGACGCAACCGCTT+TGG	0.410420	4:-6892602	MsG0480018580.01.T01:CDS
GAATGCTATTTATCAGAATC+AGG	0.425031	4:-6892508	MsG0480018580.01.T01:CDS
GTTTCGAGACCACATCCCTT+TGG	0.425269	4:+6892436	None:intergenic
TCGTGGAAACAAACGATGGC+AGG	0.437134	4:+6892858	None:intergenic
CCAGGAAAACCGAAAGATAC+TGG	0.444180	4:-6892904	MsG0480018580.01.T01:CDS
GCAGCCAAAGCGGTTGCGTC+CGG	0.447146	4:+6892598	None:intergenic
TCTTCTTCTCCAAAGGGATG+TGG	0.458927	4:-6892445	MsG0480018580.01.T01:CDS
GGTTCTTTCTCCCATGAAAA+CGG	0.468783	4:+6892925	None:intergenic
GTCGTGGAGCATGGCAGTAC+AGG	0.469526	4:+6892364	None:intergenic
CAGCGCAACTCGAGAGAGTC+GGG	0.479961	4:-6892319	MsG0480018580.01.T01:CDS
CTTTCTTCACGTTCGTGCAA+GGG	0.499824	4:+6892266	None:intergenic
TGATCATGTAAGTAGGAGAT+TGG	0.506183	4:+6892632	None:intergenic
TCTTTCTTCACGTTCGTGCA+AGG	0.523409	4:+6892265	None:intergenic
CAAAGATGTGTCTGCAACGA+CGG	0.528117	4:+6892993	None:intergenic
CTGCAGATTTGTCGTAGTCG+TGG	0.532103	4:+6892348	None:intergenic
CCGAAAGATACTGGTAGGAA+TGG	0.534229	4:-6892895	MsG0480018580.01.T01:CDS
TGATCTTTACTCAGATGCTA+TGG	0.539239	4:-6892767	MsG0480018580.01.T01:CDS
ATTATGTCAACACGTCGTCT+TGG	0.552256	4:-6893018	MsG0480018580.01.T01:CDS
TTATGCTTCATAGTTCTCCA+AGG	0.554836	4:+6892406	None:intergenic
AAACGGTTGATCATGTAAGT+AGG	0.561511	4:+6892625	None:intergenic
TTTGTTTCCACGAAAAGAAG+CGG	0.584227	4:-6892848	MsG0480018580.01.T01:CDS
CGTGGAAACAAACGATGGCA+GGG	0.601100	4:+6892859	None:intergenic
TATGCTTCATAGTTCTCCAA+GGG	0.609801	4:+6892407	None:intergenic
TTAAAGCTTGCAGAGTGTAG+TGG	0.611037	4:-6892658	MsG0480018580.01.T01:CDS
TCAGCGCAACTCGAGAGAGT+CGG	0.612829	4:-6892320	MsG0480018580.01.T01:CDS
GAAAACCGAAAGATACTGGT+AGG	0.618943	4:-6892900	MsG0480018580.01.T01:CDS
TTTGTCGTAGTCGTGGAGCA+TGG	0.626564	4:+6892355	None:intergenic
CGGTTGCGTCCGGAAGAAAA+CGG	0.640456	4:+6892608	None:intergenic
AGACGATGATGCAGCCAAAG+CGG	0.673928	4:+6892588	None:intergenic
AGTCGGGACAGAAGAAACAA+CGG	0.729959	4:-6892303	MsG0480018580.01.T01:CDS
GGAAACAAACGATGGCAGGG+TGG	0.758125	4:+6892862	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GATGTAAAAAATGATTGTGA+TGG	-	Chr4:6892459-6892478	MsG0480018580.01.T01:CDS	25.0%
	GATTCTGATAAATAGCATTC+TGG	+	Chr4:6892765-6892784	None:intergenic	30.0%
	TCTTTGTCATCAAGTTCATT+AGG	-	Chr4:6892297-6892316	MsG0480018580.01.T01:CDS	30.0%
!	AAAAAATGATTGTGATGGAG+AGG	-	Chr4:6892464-6892483	MsG0480018580.01.T01:CDS	30.0%
!	GAATGCTATTTATCAGAATC+AGG	-	Chr4:6892765-6892784	MsG0480018580.01.T01:CDS	30.0%
	AAACGGTTGATCATGTAAGT+AGG	+	Chr4:6892651-6892670	None:intergenic	35.0%
	TATGCTTCATAGTTCTCCAA+GGG	+	Chr4:6892869-6892888	None:intergenic	35.0%
	TCTTCTTCTTCTTCTCCAAA+GGG	-	Chr4:6892822-6892841	MsG0480018580.01.T01:CDS	35.0%
	TGATCATGTAAGTAGGAGAT+TGG	+	Chr4:6892644-6892663	None:intergenic	35.0%
	TGATCTTTACTCAGATGCTA+TGG	-	Chr4:6892506-6892525	MsG0480018580.01.T01:CDS	35.0%
	TTATGCTTCATAGTTCTCCA+AGG	+	Chr4:6892870-6892889	None:intergenic	35.0%
	TTCTTCTTCTTCTTCTCCAA+AGG	-	Chr4:6892821-6892840	MsG0480018580.01.T01:CDS	35.0%
	TTTGTTTCCACGAAAAGAAG+CGG	-	Chr4:6892425-6892444	MsG0480018580.01.T01:CDS	35.0%
!	TGAAAGAGTTCCGTTTTCAT+GGG	-	Chr4:6892338-6892357	MsG0480018580.01.T01:CDS	35.0%
	CCATTCCTACCAGTATCTTT+CGG	+	Chr4:6892381-6892400	None:intergenic	40.0%
	GAAAACCGAAAGATACTGGT+AGG	-	Chr4:6892373-6892392	MsG0480018580.01.T01:CDS	40.0%
	GGTTCTTTCTCCCATGAAAA+CGG	+	Chr4:6892351-6892370	None:intergenic	40.0%
	TTAAAGCTTGCAGAGTGTAG+TGG	-	Chr4:6892615-6892634	MsG0480018580.01.T01:CDS	40.0%
!	CATGATCAACCGTTTTCTTC+CGG	-	Chr4:6892656-6892675	MsG0480018580.01.T01:CDS	40.0%
!	CTTTTCGTGGAAACAAACGA+TGG	+	Chr4:6892422-6892441	None:intergenic	40.0%
!	GTGAAAGAGTTCCGTTTTCA+TGG	-	Chr4:6892337-6892356	MsG0480018580.01.T01:CDS	40.0%
!	TTTTCATGGGAGAAAGAACC+AGG	-	Chr4:6892351-6892370	MsG0480018580.01.T01:CDS	40.0%
	AGTCGGGACAGAAGAAACAA+CGG	-	Chr4:6892970-6892989	MsG0480018580.01.T01:CDS	45.0%
	CAAAGATGTGTCTGCAACGA+CGG	+	Chr4:6892283-6892302	None:intergenic	45.0%
	CCAGGAAAACCGAAAGATAC+TGG	-	Chr4:6892369-6892388	MsG0480018580.01.T01:CDS	45.0%
	CCGAAAGATACTGGTAGGAA+TGG	-	Chr4:6892378-6892397	MsG0480018580.01.T01:CDS	45.0%
	CTTTCTTCACGTTCGTGCAA+GGG	+	Chr4:6893010-6893029	None:intergenic	45.0%
	GTCTCGAAACTTTCTTCCCT+TGG	-	Chr4:6892850-6892869	MsG0480018580.01.T01:CDS	45.0%
	TCTTCTTCTCCAAAGGGATG+TGG	-	Chr4:6892828-6892847	MsG0480018580.01.T01:CDS	45.0%
	TCTTTCTTCACGTTCGTGCA+AGG	+	Chr4:6893011-6893030	None:intergenic	45.0%
!	CCAGTATCTTTCGGTTTTCC+TGG	+	Chr4:6892372-6892391	None:intergenic	45.0%
	AGACGATGATGCAGCCAAAG+CGG	+	Chr4:6892688-6892707	None:intergenic	50.0%
	CGTGGAAACAAACGATGGCA+GGG	+	Chr4:6892417-6892436	None:intergenic	50.0%
	CTGCAGATTTGTCGTAGTCG+TGG	+	Chr4:6892928-6892947	None:intergenic	50.0%
	GTTTCGAGACCACATCCCTT+TGG	+	Chr4:6892840-6892859	None:intergenic	50.0%
	TCGTGGAAACAAACGATGGC+AGG	+	Chr4:6892418-6892437	None:intergenic	50.0%
	TTTGTCGTAGTCGTGGAGCA+TGG	+	Chr4:6892921-6892940	None:intergenic	50.0%
!	TGATCAGCCGCTTCTTTTCG+TGG	+	Chr4:6892435-6892454	None:intergenic	50.0%
	CGGTTGCGTCCGGAAGAAAA+CGG	+	Chr4:6892668-6892687	None:intergenic	55.0%
	GGAAACAAACGATGGCAGGG+TGG	+	Chr4:6892414-6892433	None:intergenic	55.0%
	TCAGCGCAACTCGAGAGAGT+CGG	-	Chr4:6892953-6892972	MsG0480018580.01.T01:CDS	55.0%
	CAGCGCAACTCGAGAGAGTC+GGG	-	Chr4:6892954-6892973	MsG0480018580.01.T01:CDS	60.0%
	GTCGTGGAGCATGGCAGTAC+AGG	+	Chr4:6892912-6892931	None:intergenic	60.0%
	TCTTCCGGACGCAACCGCTT+TGG	-	Chr4:6892671-6892690	MsG0480018580.01.T01:CDS	60.0%
	GCAGCCAAAGCGGTTGCGTC+CGG	+	Chr4:6892678-6892697	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	6892258	6893037	6892258	ID=MsG0480018580.01;Name=MsG0480018580.01
Chr4	mRNA	6892258	6893037	6892258	ID=MsG0480018580.01.T01;Parent=MsG0480018580.01;Name=MsG0480018580.01.T01;_AED=0.45;_eAED=0.45;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|259
Chr4	exon	6892258	6893037	6892258	ID=MsG0480018580.01.T01:exon:1732;Parent=MsG0480018580.01.T01
Chr4	CDS	6892258	6893037	6892258	ID=MsG0480018580.01.T01:cds;Parent=MsG0480018580.01.T01

Gene Sequence

>MsG0480018580.01.T01

ATGTCAACACGTCGTCTTGGTTCCGTCGTTGCAGACACATCTTTGTCATCAAGTTCATTAGGTACATTTCAAAACACAAGTGAAAGAGTTCCGTTTTCATGGGAGAAAGAACCAGGAAAACCGAAAGATACTGGTAGGAATGGCTCGAAATTACCACCCTGCCATCGTTTGTTTCCACGAAAAGAAGCGGCTGATCAATCTGATGTAAAAAATGATTGTGATGGAGAGGATTATATTGACAATAATGATGATCTTTACTCAGATGCTATGGATGATGTGTTTTCTTTATCAGAAGCATTAGATATTGTTCAAAGAAAATCAGAGCAAGCTCATAGTGATAACAATAATAGTTTGAAGTTAAAGCTTGCAGAGTGTAGTGGCTACCAATCTCCTACTTACATGATCAACCGTTTTCTTCCGGACGCAACCGCTTTGGCTGCATCATCGTCTGCTTTAAATTTTCATAGTGATTTTGAAGAGAATGTTTGTAATACTTGTAGTTATCCAGAATGCTATTTATCAGAATCAGGTAGACATTCACATTCATACGCTTATGCTTCTTGTTCTTCTTCTTCTTCTCCAAAGGGATGTGGTCTCGAAACTTTCTTCCCTTGGAGAACTATGAAGCATAAGTTTTGTGCTATAGAGAGTCCTGTACTGCCATGCTCCACGACTACGACAAATCTGCAGAAACATCAGCGCAACTCGAGAGAGTCGGGACAGAAGAAACAACGGTCATCAGCTTACATCCCTTGCACGAACGTGAAGAAAGATGTTTGA

Protein sequence

>MsG0480018580.01.T01

MSTRRLGSVVADTSLSSSSLGTFQNTSERVPFSWEKEPGKPKDTGRNGSKLPPCHRLFPRKEAADQSDVKNDCDGEDYIDNNDDLYSDAMDDVFSLSEALDIVQRKSEQAHSDNNNSLKLKLAECSGYQSPTYMINRFLPDATALAASSSALNFHSDFEENVCNTCSYPECYLSESGRHSHSYAYASCSSSSSPKGCGLETFFPWRTMKHKFCAIESPVLPCSTTTTNLQKHQRNSRESGQKKQRSSAYIPCTNVKKDV*